WO2022127884A1

WO2022127884A1 - Pipetting apparatus having three degrees of freedom of closed cassette and closed cassette

Info

Publication number: WO2022127884A1
Application number: PCT/CN2021/138990
Authority: WO
Inventors: 袁庆明
Original assignee: 上海思路迪生物医学科技有限公司
Priority date: 2020-12-18
Filing date: 2021-12-17
Publication date: 2022-06-23
Also published as: CN112623470A; CN112623470B

Abstract

A pipetting apparatus having three degrees of freedom of a closed cassette. The closed cassette comprises a reagent tray (10). Multiple reagent holes (11) are provided on the reagent tray (10). The pipetting apparatus comprises a pipetting head (20), a rotating platform (30), a first driving component, and a second driving component. The rotating platform (30) comprises a rotating base plate (31) and a base plate supporting part (32). The rotating base plate (31) is rotatably provided on the base plate supporting part (32). The rotating base plate (31) is provided with a sliding groove (313). The pipetting head (20) is slidably provided in the sliding groove (313). The first driving component is used for driving the pipetting head (20) to slide along the sliding groove (313) and driving the rotating base plate (31) to rotate relative to the base plate supporting part (32) so as to drive the pipetting head (20) to rotate, thus allowing the pipetting head (20) to be aligned with any reagent hole (11) on the reagent tray (10). The second driving component is used for driving the rotating platform (30) to move up and down and driving the pipetting head (20) to move up and down, thus allowing the pipetting head (20) to perform a liquid drawing or liquid injecting operation with respect to the aligned reagent hole (11). The closed cassette comprises the reagent tray and the pipetting apparatus having three degrees of freedom. The pipetting apparatus having three degrees of freedom extends the degrees of freedom of the pipetting apparatus, thus allowing more reagent holes to be used in the limited volume of the closed cassette.

Description

Three degrees of freedom pipetting device for closed cartridges and closed cartridges

technical field

The invention relates to the technical field of gene sequencing, in particular to a three-degree-of-freedom pipetting device of a closed cartridge and a closed cartridge.

Background technique

Nucleic acid is the main carrier of life genetic information. DNA and RNA sequences are called genetic codes, which are the basis for analyzing gene structure, function and their interrelationships, and are also the most accurate basis for molecular diagnosis of clinical diseases. Gene sequencing is a new type of gene detection technology, which is also of great significance and value to clinical medicine. The mechanism research and clinical diagnosis of many diseases gradually rely on gene sequencing technology. Before gene sequencing, the sequencing samples need to be prepared. Initially, preparation was mostly done manually, but as the number of samples increased, so did the need for automation.

There are mainly two types of existing automated sequencing sample preparation devices. One is an open platform, which has a complex structure and a large operational throughput, but is bulky and not suitable for miniaturization, and an open platform can easily lead to cross-contamination. The other is a closed cartridge, which is small in size and is not prone to cross-contamination, and pipetting operations are all inside the cartridge.

In the related closed cartridges, a two-degree-of-freedom pipetting device is usually used, one of which is achieved by the up and down movement of the pipette, and the other is achieved by the rotation of the reagent disc. As a result, in a closed cartridge with limited volume, only one circle of reagent wells can be effectively used, which greatly limits the number of reagent wells, resulting in low preparation efficiency.

SUMMARY OF THE INVENTION

The invention provides a three-degree-of-freedom pipetting device of a closed cartridge and a closed cartridge, which expands the degree of freedom of the pipetting device, so that more reagent holes can be used in the limited volume of the closed cartridge .

A first aspect of the present invention provides a three-degree-of-freedom pipetting device for a closed cartridge, the closed cartridge includes a reagent disk, and a plurality of reagent holes are provided on the reagent disk; the pipetting device is located in Above the reagent tray, the pipetting device includes: a pipetting head, a rotating platform, a first driving component and a second driving component;

The rotating platform includes a rotating chassis and a chassis bearing part, the rotating chassis is rotatably arranged on the chassis bearing part, the rotating chassis is provided with a chute, and the pipetting head is slidably arranged on the chassis bearing part. in the chute;

The first drive assembly and the second drive assembly are arranged on the chassis bearing part; the first drive assembly is used to drive the pipetting head to slide along the chute, and to drive the rotating chassis to be relatively The chassis bearing part rotates to drive the pipetting head to rotate, so that the pipetting head can be aligned with any reagent hole on the reagent tray; the second driving component is used to drive the rotating platform to move up and down and drive all the The pipetting head moves up and down, so that the pipetting head can perform liquid suction or liquid injection operations for the aligned reagent holes.

According to an embodiment of the present invention,

The rotating chassis includes a first chassis part and a second chassis part, and the first chassis part and the second chassis part are located on both sides of the chute;

the first drive assembly includes a first drive shaft, a first rope, a second drive shaft and a second rope; the first drive shaft and the second drive shaft are rotatably disposed on the chassis bearing part;

The first rope is wound around the first drive shaft and the first chassis part, and is connected with the pipetting head, and can slide relative to the first chassis part and drive the first drive shaft when the first drive shaft rotates The pipette head slides along the chute;

The second rope is wound around the second drive shaft and the second chassis portion, and is connected to the pipetting head, and can drive the rotating chassis to rotate and drive the pipetting when the second drive shaft rotates The head slides along the chute.

According to an embodiment of the present invention,

The arc surface of the first chassis part is provided with a plurality of rollers, and the first rope slides relative to the first chassis part through the rollers;

The arc surface of the second chassis part is in direct contact with the second rope, and the second rope drives the second chassis part to rotate through the friction force between the second rope and the second chassis part.

According to an embodiment of the present invention,

The first drive assembly further includes a first roller combination and a second roller combination;

The first roller combination includes at least two rollers; the first roller combination is arranged on the chassis bearing part and located between the first drive shaft and the first chassis part, and is used for positioning the first rope on the The part between the first drive shaft and the first chassis part is limited between the two rollers of the first roller combination;

The second roller combination includes at least two rollers; the second roller combination is arranged on the chassis bearing part and located between the second drive shaft and the second chassis part, and is used for placing the second rope on the chassis The portion between the second drive shaft and the second chassis portion is limited between the two rollers of the second roller assembly.

According to an embodiment of the present invention,

The distance between the two rollers of the first roller combination is less than or equal to the diameter of the first drive shaft;

The distance between the two rollers of the second roller combination is less than or equal to the diameter of the second drive shaft.

According to an embodiment of the present invention,

The chassis bearing part has N lead screw nuts arranged at equal intervals, and the N is greater than or equal to 3;

The second drive assembly includes N lead screws, which are respectively inserted into the N lead screw nuts, and drive the rotating platform to move up and down through relative rotation with the N lead screw nuts.

According to an embodiment of the present invention,

One end of the N lead screws is rotatably connected to the fixed position of the reagent tray; the other end of the N lead screws is connected to an external drive device, and the external drive device is used to drive the N lead screws Synchronous and co-rotating.

According to an embodiment of the present invention, the pipetting device further includes a pump assembly, and the vent hole of the pump assembly is connected to the vent hole of the pipetting head through a hose.

According to an embodiment of the present invention,

The pump assembly includes a housing and a piston, and the piston can move up and down within the housing to pump air through the vent hole of the pump assembly, the hose, and the vent hole of the pipetting tip for the pipetting head or the pipetting tip. Inflating to make the pipette head perform liquid suction or liquid injection operation;

The end of the piston is provided with a sealing ring, and the sealing ring is in the shape of a dumbbell.

According to an embodiment of the present invention,

The vent hole of the pump assembly is bent.

A second aspect of the present invention provides a closed cartridge, comprising a reagent tray and the three-degree-of-freedom pipetting device of the closed cartridge as described in the foregoing embodiments.

The present invention has the following beneficial effects:

In the embodiment of the present invention, the horizontal movement and rotation of the pipetting head can be realized through the cooperation of the first drive assembly and the rotating platform, and the vertical movement of the pipetting head can be realized through the cooperation of the second drive assembly and the rotating platform. The whole pipetting device can realize the three-degree-of-freedom movement of the pipetting head, which expands the degree of freedom of the pipetting device. Moreover, the pipetting head is set on the chute of the rotating chassis included in the rotating platform, and the three-degree-of-freedom movements are all It can be completed around the rotating platform, and the structure is relatively compact, so that the pipette head can have more freedom of movement within the limited volume of the closed cartridge, so that more reagent holes can be used, which is conducive to improving the sample Preparation efficiency.

Description of drawings

In order to illustrate the embodiments of the present invention or the technical solutions in the prior art more clearly, the following briefly introduces the accompanying drawings that are used in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only These are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without any creative effort.

1 is a schematic perspective view of a closed cartridge according to an embodiment of the present invention;

2 is a schematic structural diagram of a closed cartridge according to an embodiment of the present invention after the outer casing is removed;

3 is a schematic structural diagram of a pipetting device according to an embodiment of the present invention;

4 is a schematic diagram of the working principle of a pipetting device according to an embodiment of the present invention;

5 is a schematic structural diagram of a reagent disk according to an embodiment of the present invention;

6 is a schematic diagram of the connection structure of the pump assembly and the pipetting head according to an embodiment of the present invention;

7 is a schematic cross-sectional structural diagram of a pump assembly according to an embodiment of the present invention.

Description of reference numbers:

Reagent plate 10; reagent hole 11; pipetting head 20; rotating platform 30; rotating chassis 31; first chassis part 311; first roller 3111; second chassis part 312; screw nut 321; second screw nut 322; third screw nut 323; first drive assembly 40; first drive shaft 41; first rope 42; second drive shaft 43; second rope 44; second roller 45 The third roller 46; the second drive assembly 50; the first screw 51; the second screw 52; the third screw 53; the pump assembly 60; 70; hot cover 80; hose 90.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

The terms "first", "second", "third", "fourth", etc. (if present) in the description and claims of the present invention and the above-mentioned drawings are used to distinguish similar objects and are not necessarily used to Describe a particular order or sequence. It is to be understood that the data so used may be interchanged under appropriate circumstances such that the embodiments of the invention described herein can be practiced in sequences other than those illustrated or described herein. Furthermore, the terms "comprising" and "having" and any variations thereof, are intended to cover non-exclusive inclusion, for example, a process, method, system, product or device comprising a series of steps or units is not necessarily limited to those expressly listed Rather, those steps or units may include other steps or units not expressly listed or inherent to these processes, methods, products or devices.

The technical solutions of the present invention will be described in detail below with specific examples. The following specific embodiments may be combined with each other, and the same or similar concepts or processes may not be repeated in some embodiments.

1 and 2 , in one embodiment, the closed cartridge includes a reagent tray 10 and a three-degree-of-freedom pipetting device, and the pipetting device is located above the reagent tray 10 . The three-DOF pipetting device is shown in Figures 3 and 4. The reagent disk 10 is shown in FIG. 5 , and the reagent disk 10 is provided with a plurality of reagent wells 11 .

Of course, the closed cartridge may also have other components, for example, it may also include the housing 70 shown in FIG. The heating cover 80 and the like for heating and keeping a part of the reagent wells 11 are not specifically limited.

Continuing to refer to FIGS. 1-4 , the pipetting device includes: a pipetting head 20 , a rotating platform 30 , a first driving assembly 40 and a second driving assembly 50 . The pipetting head 20 can be arranged on the rotating platform 30, and the pipetting head 20 can be moved with three degrees of freedom through the rotating platform 30, the first driving assembly 40 and the second driving assembly 50, including horizontal movement, rotation and vertical movement. Movement in a straight direction.

The rotating platform 30 includes a rotating chassis 31 and a chassis bearing portion 32 . The rotating chassis 31 is rotatably arranged on the chassis bearing portion 32 , and the rotating chassis 31 is provided with a chute 313 , and the pipetting head 20 is slidably arranged on the chute. 313.

The first drive assembly 40 and the second drive assembly 50 are arranged on the chassis carrying part 32; the first drive assembly 40 is used to drive the pipetting head 20 to slide along the chute 313, and to drive the rotating chassis 31 to rotate relative to the chassis carrying part 32 to The pipette head 20 is driven to rotate, so that the pipette head 20 can be aligned with any reagent hole 11 on the reagent tray 10; the second drive assembly 50 is used to drive the rotating platform 30 to move up and down and drive the pipette head 20 to move up and down, so that the pipette head 20 is capable of liquid aspiration or liquid injection operation for the aligned reagent wells 11 .

In other words, the first drive assembly 40 cooperates with the rotating chassis 31 to enable the pipetting head 20 to move and rotate relative to the reagent tray 10 in the horizontal direction, and the second drive assembly 50 cooperates with the rotating chassis 31 to enable the pipetting head 20 to move relative to the reagent tray. 10 moves in the vertical direction.

First, the cooperation between the first drive assembly 40 and the rotating chassis 31 is described, so that the pipetting head 20 moves and rotates in the horizontal direction relative to the reagent tray 10 .

Optionally, the rotating chassis 31 includes a first chassis portion 311 and a second chassis portion 312 , and the first chassis portion 311 and the second chassis portion 312 are located on both sides of the chute 313 . The rotating chassis 31 may have a circular shape, and is divided into two parts with the chute 313 as the boundary, which are the first chassis part 311 and the second chassis part 312 respectively.

Of course, there may still be a connection relationship between the first chassis portion 311 and the second chassis portion 312 , for example, the chute 313 does not directly divide the rotating chassis 31 into two halves. The chassis carrying portion 32 can also be circular, and of course it can also have an edge extending upward in the vertical direction (here, the upward extension is preferred, but it is not limited, for example, it can also extend downward), so as to rotate The chassis 31 is limited therein.

The first chassis portion 311 and the second chassis portion 312 can rotate relative to the chassis carrying portion 32, but they need to be driven by external force. Under the driving of external force, if the second chassis portion 312 rotates, it will inevitably drive the first chassis portion 311 to rotate. , that is, the entire rotating chassis 31 is driven to rotate, and the pipetting head 20 is also rotated.

The first drive assembly 40 may include a first drive shaft 41 , a first cable 42 , and a second drive shaft 43 and a second cable 44 . The first drive shaft 41 and the second drive shaft 43 are rotatably disposed on the chassis bearing portion 32 . At least one end of the first drive shaft 41 and the second drive shaft 43 can be limited on the housing 70 or the reagent disc 10, so that they can rotate relative to the housing 70 and the reagent disc 10, but cannot move up and down, so as to ensure that the pipetting device is in place. Stablize.

The first rope 42 is wound around the first drive shaft 41 and the first chassis part 311 and is connected to the pipetting head 20 , and can slide relative to the first chassis part 311 and drive the pipetting head 20 when the first drive shaft 41 rotates Slide along the chute 313 .

In other words, when the first drive shaft 41 rotates and pulls the first rope 42, the first rope 42 is not enough to drive the first chassis portion 311 to rotate, that is, it is not enough to drive the rotating chassis 31 to rotate. At this time, the rotating chassis 31 remains stationary, but the first A rope 42 can pull the pipetting head 20 to slide along the chute 313 , that is, the pipetting head 20 can move linearly in the horizontal direction.

Optionally, as shown in FIG. 4 , the arc surface of the first chassis portion 311 is provided with a plurality of first rollers 3111 , and the first rope 42 slides relative to the first chassis portion 311 through the first rollers 3111 (for ease of understanding, FIG. 4, the distances between the first drive shaft 41, the second drive shaft 43 and the chassis bearing part 32 have been adjusted to increase the distance between the first drive shaft 41, the second drive shaft 43 and the chassis bearing part 32 , resulting in the visual appearance that the first drive shaft 41 and the second drive shaft 43 are detached from the chassis bearing portion 32, but actually the first drive shaft 41 and the second drive shaft 43 can be close to and connected to the chassis bearing portion 32).

When the first drive shaft 41 rotates, the first rope 42 will be pulled. Due to the existence of the first roller 3111 on the arc surface of the first chassis portion 311, the contact area between the first rope 42 and the arc surface of the first chassis portion 311 is small. Even without contact, the first rope 42 cannot drive the first chassis portion 311 to rotate, but only slides relative to the first chassis portion 311 . Since the first rope 42 is connected with the pipetting head 20 , when the first rope 42 slides, the pipetting head 20 will be pulled to slide along the chute 313 .

A plurality of first rollers 3111 can be arranged on the arc surface of the first chassis portion 311 at intervals, and the number of the first rollers 3111 is not limited, as shown in FIG. The entire arc surface of the first chassis portion 311 is covered. Of course, there is no specific limitation.

It can be understood that it is preferable to set the first roller 3111 on the arc surface of the first chassis part 311 to realize the sliding of the first rope 42 relative to the first chassis part 311, but it is not a limitation. Others can realize the first rope The manner in which 42 slides relative to the first chassis portion 311 is applicable.

The second rope 44 is wound around the second drive shaft 43 and the second chassis portion 312 , and can drive the rotating chassis 31 to rotate when the second drive shaft 43 rotates, and drive the rotating chassis 31 when the second drive shaft 43 rotates counterclockwise. (ie, the first chassis portion 311 and the second chassis portion 312, both of which are integrated) rotate counterclockwise. At this time, if the first drive shaft 41 does not rotate, since the rotating chassis 31 is moving, there will be relative sliding between the first rope 42 and the rotating chassis 31, and the pipetting head 20 and the first rope 42 are fixedly connected, so The pipetting head 20 also slides relative to the rotating chassis 31 , specifically, slides down along the chute 313 , where the down direction is the opposite direction of the straight arrow in FIG. 4 . If the first drive shaft 41 also rotates counterclockwise, the pipette head 20 will be pulled upward (ie, in the direction of the straight arrow in FIG. 4 ). By controlling the rotational speeds of the first drive shaft 41 and the second drive shaft 43 , the The pipetting head 20 is controlled to be stationary relative to the rotating chassis 31, or to slide up or down, depending on the rotational speed.

In other words, when the second drive shaft 43 rotates and pulls the second rope 44, the second rope 44 can drive the second chassis portion 312 to rotate, that is, drive the entire rotating chassis 31 to rotate. At the same time, through the first drive shaft 41, the first rope 42, the pipette head 20 can be controlled to slide along the chute 313, that is, the first drive assembly 40 realizes the rotation of the pipette head 20 in the horizontal direction on the one hand, and realizes the rotation of the pipette head 20 in the horizontal direction on the other hand. The linear movement of two degrees of freedom is realized.

Optionally, as shown in FIG. 4 , the arc surface of the second chassis part 312 is in direct contact with the second rope 44 , and the second rope 44 drives the second chassis part 312 to rotate through the frictional force between the second chassis part 312 and the second chassis part 312 . .

When the second driving shaft 43 rotates, it will pull the second rope 44. Since the arc surface of the second chassis portion 312 is in direct contact with the second rope 44, when the second rope 44 is pulled, the arc-shaped surface of the second chassis portion 312 is in direct contact with the second rope 44. The surface and the second rope 44 will generate a large frictional force, so that the second rope 44 can pull the second chassis portion 312 to rotate, and also pull the entire rotating chassis 31 to rotate.

The first drive shaft 41 and the second drive shaft 43 can be connected to an external drive device, and are driven to rotate by the external drive device, which is not specifically limited.

Optionally, the first driving assembly 40 further includes a first roller combination and a second roller combination.

Continuing to refer to FIG. 4 , the first roller combination includes two second rollers 45 , of course, this is only preferred, and the actual first roller combination may also include more rollers. The two second rollers 45 are disposed on the chassis carrying portion 32 and located between the first drive shaft 41 and the first chassis portion 311 . Specifically, the shafts of the two second rollers 45 are fixed on the chassis carrying portion 32 , and the two second The opposite side of the roller 45 goes around the first rope 42 . The two second rollers 45 are used to limit the part of the first rope 42 between the first drive shaft 41 and the first chassis part 311 between the two second rollers 45, so as to ensure that the first rope 42 can be connected with more contact with the first roller 3111.

The second roller combination includes two third rollers 46, of course, this is only preferred, and the actual second roller combination may also include more rollers. The two third rollers 46 are disposed on the chassis carrying portion 32 and located between the second drive shaft 43 and the second chassis portion 312 . Specifically, the shafts of the two third rollers 46 are fixed on the chassis carrying portion 32 , and the two third The opposite side of the roller 46 goes around the second rope 44 . The two third rollers 46 are used to limit the part of the second rope 44 between the second drive shaft 43 and the second chassis part 312 between the two rollers of the second roller combination, so as to ensure that the second rope 44 can be connected with the second roller. There is more contact with the arcuate surface of the second chassis portion 312 .

Optionally, the distance between the two second rollers 45 is less than or equal to the diameter of the first drive shaft 41 ; the distance between the two third rollers 46 is less than or equal to the diameter of the second drive shaft 43 . Here, the distance between the two second rollers 45 refers to the distance between the edges of the two second rollers 45, more specifically the shortest distance between the edges of the two second rollers 45; The distance between the three rollers 46 refers to the distance between the edges of the two third rollers 46 , and more specifically, the shortest distance between the edges of the two third rollers 46 .

In this way, the contact range between the first rope 42 and the first roller 3111 and the second rope 44 and the second chassis part 312 can be made larger, so as to control the rotation of the first rope 42 and the second rope 44 more effectively, and also Avoid rope entanglement. Of course, this is only a preference, not a limitation.

Next, it will be explained that the second driving assembly 50 cooperates with the rotating chassis 31 to make the pipetting head 20 move in the vertical direction relative to the reagent tray 10 .

Optionally, the chassis bearing portion 32 has N lead screw nuts arranged at equal intervals, and N is greater than or equal to 3. The second driving assembly 50 includes N lead screws, respectively passing through the N lead screw nuts, and drives the rotating platform 30 to move up and down through relative rotation with the N lead screw nuts.

Optionally, one end of the N lead screws is rotatably connected to the fixed position of the reagent tray 10; the other end of the N lead screws is connected to an external drive device, and the external drive device is used to drive the N lead screws to rotate synchronously and in the same direction. .

Referring to FIG. 3 , the chassis bearing portion 32 has three lead screw nuts, namely the first lead screw 51 nut 321, the second lead screw 52 nut 322, the third lead screw 53 nut 323, the first lead screw 51 nut 321, the first lead screw 51 nut 321, the third lead screw 53 nut 323, The intervals between the nuts 322 of the second lead screw 52 and the nuts 323 of the third lead screw 53 are equal. The first lead screw 51 nut 321, the second lead screw 52 nut 322, and the third lead screw 53 nut 323 are respectively provided with a first lead screw 51, a second lead screw 52, a third lead screw 53, and the first lead screw 51 The lower ends of the second lead screw 52 and the third lead screw 53 are rotatably connected to the fixed position of the reagent tray 10, and the positional relationship with the reagent tray 10 remains unchanged during rotation. The first lead screw 51 and the second lead screw 52. The upper end of the third lead screw 53 can extend out of the casing 70 to be connected with an external driving device.

The first lead screw 51, the second lead screw 52, and the third lead screw 53 have external threads, and the first lead screw 51 nut 321, the second lead screw 52 nut 322, and the third lead screw 53 nut 323 have internal threads. The existence of the first lead screw 51, the second lead screw 52, and the third lead screw 53 rotate relative to the first lead screw 51 nut 321, the second lead screw 52 nut 322, and the third lead screw 53 nut 323, respectively, the rotating platform 30 will move up and down, so as to realize the up and down movement of the pipetting head 20 in the vertical direction.

In order to ensure that the rotating platform 30 is always level during the movement, the first lead screw 51 , the second lead screw 52 and the third lead screw 53 need to rotate synchronously and in the same direction. For example, when synchronously rotating counterclockwise, the rotating platform 30 moves downward; when synchronously rotating clockwise, the rotating platform 30 moves upward. For example, the synchronization here refers to the synchronization of rotation speed, start rotation, and end rotation.

In the above-mentioned embodiment, the horizontal movement and rotation of the pipetting head 20 can be realized through the cooperation of the first driving assembly 40 and the rotating platform 30 , and the pipetting head can be realized through the cooperation of the second driving assembly 50 and the rotating platform 30 . 20 vertical movement, whereby the entire pipetting device can realize the three-degree-of-freedom movement of the pipetting head 20, which expands the degree of freedom of the pipetting device, and the pipetting head 20 is arranged on the rotating chassis 31 included in the rotating platform 30. On the chute 313, the movement of three degrees of freedom can be completed around the rotating platform 30, which is relatively compact in structure, so that the pipetting head 20 can have more moving degrees of freedom in the limited volume of the closed cartridge, thereby More reagent wells 11 can be used, which is beneficial to improve the efficiency of sample preparation.

In addition, during the movement of the pipetting head 20, the reagent tray 10 can always remain stationary, which can also ensure the stability of the reagents in the reagent wells 11 and avoid problems such as reagent shaking.

It can be understood that the specific structures of the above-mentioned rotating platform 30 , the first driving assembly 40 and the second driving assembly 50 are only preferred modes, and are not actually limited thereto.

In one embodiment, referring to FIGS. 1 to 2 and FIGS. 6 to 7 , the pipetting device further includes a pump assembly 60 , and the vent hole 611 of the pump assembly 60 is connected to the vent hole 611 of the pipetting head 20 through a hose 90 .

When the pump assembly 60 inflates the pipette head 20 through the hose 90, the pipette head 20 can realize the liquid injection operation. When the pump assembly 60 sucks the gas of the pipetting head 20 through the hose 90 , the pipetting head 20 can realize the liquid suction operation. That is, through the pump assembly 60 and the hose 90 , a desired liquid suction or liquid injection operation can be achieved while the pipetting head 20 is aligned with a certain reagent well 11 .

As shown in Figures 1 and 2, the pump assembly 60 may be mounted on the housing 70, outside the rotating platform 30, since the pump assembly 60 and the pipetting head 20 are connected by the hose 90, the movement of the pipetting head 20 is less Constrained by pump assembly 60 .

In this embodiment, the pipetting head 20 is no longer directly mounted on the pump assembly 60, but is connected through a hose 90, which can make the movement of the pipetting head 20 more flexible.

Optionally, referring to FIG. 6 and FIG. 7 , the pump assembly 60 includes a housing 61 and a piston 62 . The piston 62 can move up and down in the housing 61 to pass through the vent hole 611 of the pump assembly 60 , the hose 90 , and pipette liquid. The vent hole 611 of the head 20 is used to pump or inflate the pipetting head 20, so as to prompt the pipetting head 20 to perform a liquid suction or liquid injection operation.

The end of the piston 62 is provided with a sealing ring 621, and the sealing ring 621 is in the shape of a dumbbell. The dumbbell-shaped sealing ring 621 is equivalent to two conjoined sealing rings 621. Compared with a single sealing ring 621, it not only has good sealing performance, but also is not easily deformed. Ring 621 can also seal normally.

Optionally, the ventilation hole 611 of the pump assembly 60 is bent. As shown in FIG. 6 and FIG. 7 , the ventilation hole 611 extends from the lower end of the housing 61 , and is first bent 90 degrees horizontally, and then bent upwards 90 degrees, so that the final orientation of the ventilation hole 611 is the same as that of the housing 61 . The lower end faces in the opposite direction. Since the vent hole 611 of the pump housing 61 is bent, the piston 62 can be lowered and the pipette head 20 can be inflated, and the air flow into the hose 90 can be buffered and redirected by the vent hole 611, which can avoid the hose 90 Problems such as kinks occur.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: The technical solutions described in the foregoing embodiments can still be modified, or some or all of the technical features thereof can be equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the embodiments of the present invention. scope.

Claims

A three-degree-of-freedom pipetting device for a closed cartridge, characterized in that the closed cartridge includes a reagent disk, and the reagent disk is provided with a plurality of reagent holes; the pipetting device is located in the reagent disk Above, the pipetting device includes: a pipetting head, a rotating platform, a first driving component and a second driving component;

The rotating platform includes a rotating chassis and a chassis bearing part, the rotating chassis is rotatably arranged on the chassis bearing part, the rotating chassis is provided with a chute, and the pipetting head is slidably arranged on the chassis bearing part. in the chute;

The first drive assembly and the second drive assembly are arranged on the chassis bearing part; the first drive assembly is used to drive the pipetting head to slide along the chute, and to drive the rotating chassis to be relatively The chassis bearing part rotates to drive the pipetting head to rotate, so that the pipetting head can be aligned with any reagent hole on the reagent tray; the second driving component is used to drive the rotating platform to move up and down and drive all the The pipetting head moves up and down, so that the pipetting head can perform liquid suction or liquid injection operations for the aligned reagent holes.
The three-degree-of-freedom pipetting device for a closed cartridge according to claim 1, wherein:

The rotating chassis includes a first chassis part and a second chassis part, and the first chassis part and the second chassis part are located on both sides of the chute;

the first drive assembly includes a first drive shaft, a first rope, a second drive shaft and a second rope; the first drive shaft and the second drive shaft are rotatably disposed on the chassis bearing part;

The first rope is wound around the first drive shaft and the first chassis part, and is connected with the pipetting head, and can slide relative to the first chassis part and drive the first drive shaft when the first drive shaft rotates The pipette head slides along the chute;

The second rope is wound around the second drive shaft and the second chassis portion, and can drive the rotating chassis to rotate when the second drive shaft rotates.
The three-degree-of-freedom pipetting device for a closed cartridge according to claim 2, wherein:

The arc surface of the first chassis part is provided with a plurality of rollers, and the first rope slides relative to the first chassis part through the rollers;

The arc surface of the second chassis part is in direct contact with the second rope, and the second rope drives the second chassis part to rotate through the frictional force between the second rope and the second chassis part.
The three-degree-of-freedom pipetting device for a closed cartridge according to claim 2, wherein:

The first drive assembly further includes a first roller combination and a second roller combination;

The first roller combination includes at least two rollers; the first roller combination is arranged on the chassis bearing part and located between the first drive shaft and the first chassis part, and is used for positioning the first rope on the The part between the first drive shaft and the first chassis part is limited between the two rollers of the first roller combination;

The second roller combination includes at least two rollers; the second roller combination is arranged on the chassis bearing part and located between the second drive shaft and the second chassis part, and is used for placing the second rope on the chassis The portion between the second drive shaft and the second chassis portion is limited between the two rollers of the second roller assembly.
The three-degree-of-freedom pipetting device for a closed cartridge according to claim 4, characterized in that:

The distance between the two rollers of the first roller combination is less than or equal to the diameter of the first drive shaft;

The distance between the two rollers of the second roller combination is less than or equal to the diameter of the second drive shaft.
The three-degree-of-freedom pipetting device for a closed cartridge according to claim 1, wherein:

The chassis bearing part has N lead screw nuts arranged at equal intervals, and the N is greater than or equal to 3;

The second drive assembly includes N lead screws, which are respectively inserted into the N lead screw nuts, and drive the rotating platform to move up and down through relative rotation with the N lead screw nuts.
The three-degree-of-freedom pipetting device for a closed cartridge according to claim 6, wherein:

One end of the N lead screws is rotatably connected to the fixed position of the reagent tray; the other end of the N lead screws is connected to an external drive device, and the external drive device is used to drive the N lead screws Synchronous and co-rotating.
The three-degree-of-freedom pipetting device of a closed cartridge according to claim 1, characterized in that, the pipetting device further comprises a pump assembly, and the ventilation hole of the pump assembly is connected to the pipetting head through a hose. Air vent.
The three-degree-of-freedom pipetting device for a closed cartridge according to claim 8, wherein:

The pump assembly includes a housing and a piston, and the piston can move up and down within the housing to pump air through the vent hole of the pump assembly, the hose, and the vent hole of the pipetting head for the pipetting head or the pipetting head. Inflating to make the pipetting head perform liquid suction or liquid injection operation;

The end of the piston is provided with a sealing ring, and the sealing ring is in the shape of a dumbbell.
The three-degree-of-freedom pipetting device for a closed cartridge according to claim 8, wherein:

The vent hole of the pump assembly is bent.
A closed cartridge is characterized by comprising a reagent disk and the three-degree-of-freedom pipetting device of the closed cartridge according to any one of claims 1-10.