WO2022125700A1 - Gènes ayant des rôles dans le mécanisme de concentration de co2 d'algues, et autres - Google Patents

Gènes ayant des rôles dans le mécanisme de concentration de co2 d'algues, et autres Download PDF

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Publication number
WO2022125700A1
WO2022125700A1 PCT/US2021/062469 US2021062469W WO2022125700A1 WO 2022125700 A1 WO2022125700 A1 WO 2022125700A1 US 2021062469 W US2021062469 W US 2021062469W WO 2022125700 A1 WO2022125700 A1 WO 2022125700A1
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WO
WIPO (PCT)
Prior art keywords
seq
phenotype
plant
nos
polypeptide
Prior art date
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PCT/US2021/062469
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English (en)
Inventor
Martin C. JONIKAS
Friedrich Alexander FAUSER
Robert Edward JINKERSON
Josep Vilarrasa BLASI
Original Assignee
The Trustees Of Princeton University
The Regents Of The University Of California
The Board Of Trustees Of The Leland Stanford Junior University
Carnegie Institution Of Washington
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Application filed by The Trustees Of Princeton University, The Regents Of The University Of California, The Board Of Trustees Of The Leland Stanford Junior University, Carnegie Institution Of Washington filed Critical The Trustees Of Princeton University
Publication of WO2022125700A1 publication Critical patent/WO2022125700A1/fr

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1079Screening libraries by altering the phenotype or phenotypic trait of the host
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12Leaves
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/02Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Environmental Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne un criblage du génome de Chlamydomonas qui fournit plus de 700 relations gène/phénotype à haut niveau de confiance. L'identification de ces relations permet l'utilisation des gènes pour influencer le phénotype correspondant dans un organisme hétérologue, tel qu'une plante ou un autre organisme photosynthétique. Des aspects supplémentaires de la présente divulgation concernent des plantes génétiquement modifiées ayant un polypeptide hétérologue auquel un phénotype est maintenant lié, ou ayant un homologue natif modifié de ce polypeptide (par exemple, une inactivation ou une autre modification qui réduit ou augmente l'expression). D'autres aspects de la présente divulgation concernent des procédés de fabrication de telles plantes ainsi que la culture de ces plantes génétiquement modifiées.
PCT/US2021/062469 2020-12-09 2021-12-08 Gènes ayant des rôles dans le mécanisme de concentration de co2 d'algues, et autres WO2022125700A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063123422P 2020-12-09 2020-12-09
US63/123,422 2020-12-09

Publications (1)

Publication Number Publication Date
WO2022125700A1 true WO2022125700A1 (fr) 2022-06-16

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ID=81973976

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/062469 WO2022125700A1 (fr) 2020-12-09 2021-12-08 Gènes ayant des rôles dans le mécanisme de concentration de co2 d'algues, et autres

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WO (1) WO2022125700A1 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030233670A1 (en) * 2001-12-04 2003-12-18 Edgerton Michael D. Gene sequences and uses thereof in plants
WO2011034968A1 (fr) * 2009-09-15 2011-03-24 Sapphire Energy, Inc. Organismes tolérants au sel

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030233670A1 (en) * 2001-12-04 2003-12-18 Edgerton Michael D. Gene sequences and uses thereof in plants
WO2011034968A1 (fr) * 2009-09-15 2011-03-24 Sapphire Energy, Inc. Organismes tolérants au sel

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProtKB [online] 28 March 2018 (2018-03-28), "NAM-associated domain-containing protein {ECO:0000259|Pfam:PF14303}", XP055949591, retrieved from UniProt Database accession no. A0A2K3DMK6 *

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