WO2022114767A1 - Aptamère d'arn se liant spécifiquement à l'arn-polymérase arn dépendante du coronavirus et utilisation associée - Google Patents

Aptamère d'arn se liant spécifiquement à l'arn-polymérase arn dépendante du coronavirus et utilisation associée Download PDF

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Publication number
WO2022114767A1
WO2022114767A1 PCT/KR2021/017375 KR2021017375W WO2022114767A1 WO 2022114767 A1 WO2022114767 A1 WO 2022114767A1 KR 2021017375 W KR2021017375 W KR 2021017375W WO 2022114767 A1 WO2022114767 A1 WO 2022114767A1
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WO
WIPO (PCT)
Prior art keywords
rna
coronavirus
rdrp
rna aptamer
aptamer
Prior art date
Application number
PCT/KR2021/017375
Other languages
English (en)
Korean (ko)
Inventor
이승재
안지영
이상희
박가영
조희영
Original Assignee
주식회사 에이치피바이오
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from KR1020210162637A external-priority patent/KR20220073672A/ko
Application filed by 주식회사 에이치피바이오 filed Critical 주식회사 에이치피바이오
Publication of WO2022114767A1 publication Critical patent/WO2022114767A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/548—Carbohydrates, e.g. dextran
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Definitions

  • the present invention provides a coronavirus RNA-dependent RNA polymerase detection method comprising the step of reacting the RNA aptamer with a sample.
  • the present invention provides a health functional food for preventing or improving coronavirus infection containing the RNA aptamer as an active ingredient.
  • RNA aptamer obtained was 924.6 ng/ ⁇ l, respectively, and most specifically bound to RdRp.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La présente invention concerne : un aptamère d'ARN se liant spécifiquement à une ARN-polymérase ARN dépendante d'un coronavirus et une utilisation associée. En particulier, l'aptamère d'ARN selon la présente invention se lie spécifiquement à une ARN polymérase ARN-dépendante du coronavirus, en particulier au nouveau coronavirus provoquant la nouvelle maladie infectieuse du coronavirus (COVID-19) et, en tant que tel, peut être avantageusement utilisé pour détecter et diagnostiquer un coronavirus et traiter les maladies provoquées par celui-ci.
PCT/KR2021/017375 2020-11-25 2021-11-24 Aptamère d'arn se liant spécifiquement à l'arn-polymérase arn dépendante du coronavirus et utilisation associée WO2022114767A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20200160109 2020-11-25
KR10-2020-0160109 2020-11-25
KR10-2021-0162637 2021-11-23
KR1020210162637A KR20220073672A (ko) 2020-11-25 2021-11-23 코로나바이러스의 rna 의존성 rna 중합효소에 특이적으로 결합하는 rna 앱타머 및 이의 용도

Publications (1)

Publication Number Publication Date
WO2022114767A1 true WO2022114767A1 (fr) 2022-06-02

Family

ID=81754618

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2021/017375 WO2022114767A1 (fr) 2020-11-25 2021-11-24 Aptamère d'arn se liant spécifiquement à l'arn-polymérase arn dépendante du coronavirus et utilisation associée

Country Status (1)

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WO (1) WO2022114767A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116144665A (zh) * 2022-12-30 2023-05-23 中国人民解放军军事科学院军事医学研究院 核酸适配体在特异性识别冠状病毒中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180007919A (ko) * 2016-07-14 2018-01-24 충북대학교 산학협력단 혈소판 유래 생장 인자 수용체 베타 단백질에 특이적으로 결합하는 dna 앱타머 및 이의 용도
KR20200123753A (ko) * 2019-04-22 2020-10-30 포항공과대학교 산학협력단 신규한 등온 단일 반응용 프로브 세트 및 이의 용도

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180007919A (ko) * 2016-07-14 2018-01-24 충북대학교 산학협력단 혈소판 유래 생장 인자 수용체 베타 단백질에 특이적으로 결합하는 dna 앱타머 및 이의 용도
KR20200123753A (ko) * 2019-04-22 2020-10-30 포항공과대학교 산학협력단 신규한 등온 단일 반응용 프로브 세트 및 이의 용도

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ACQUAH CALEB; JEEVANANDAM JAISON; TAN KEI XIAN; DANQUAH MICHAEL K.: "Engineered Aptamers for Enhanced COVID-19 Theranostics", CELLULAR AND MOLECULAR BIOENGINEERING, vol. 14, no. 3, 15 January 2021 (2021-01-15), Cham, pages 209 - 221, XP037472156, ISSN: 1865-5025, DOI: 10.1007/s12195-020-00664-7 *
CHEN ZHIQIANG; WU QIHAN; CHEN JING; NI XIAOHUA; DAI JIANFENG: "A DNA Aptamer Based Method for Detection of SARS-CoV-2 Nucleocapsid Protein", VIROLOGICA SINICA, vol. 35, no. 3, 25 May 2020 (2020-05-25), DE , pages 351 - 354, XP037184841, ISSN: 1674-0769, DOI: 10.1007/s12250-020-00236-z *
DATABASE PROTEIN 18 July 2020 (2020-07-18), ANONYMOUS: "RNA-dependent RNA polymerase [Severe acute respiratory syndrome coronavirus 2]", XP055934032, retrieved from GENBANK Database accession no. YP_009725307 *
JAIN SANYA, SINGH SOMESH PRATAP, MAYYA CHAITHRA, MAJUMDAR SHARMISTHA, BHATIA DHIRAJ: "DNA Aptamers in COVID-19 Research", CURRENT SCIENCE, vol. 119, no. 9, 10 November 2020 (2020-11-10), IN , pages 1489 - 1498, XP055934014, ISSN: 0011-3891, DOI: 10.18520/cs/v119/i9/1489-1498 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116144665A (zh) * 2022-12-30 2023-05-23 中国人民解放军军事科学院军事医学研究院 核酸适配体在特异性识别冠状病毒中的应用

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