WO2022099504A1 - Procédé d'ajout de liquide pour échantillonnage et prélèvement de réactif, et système de trajet de liquide correspondant - Google Patents

Procédé d'ajout de liquide pour échantillonnage et prélèvement de réactif, et système de trajet de liquide correspondant Download PDF

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WO2022099504A1
WO2022099504A1 PCT/CN2020/128107 CN2020128107W WO2022099504A1 WO 2022099504 A1 WO2022099504 A1 WO 2022099504A1 CN 2020128107 W CN2020128107 W CN 2020128107W WO 2022099504 A1 WO2022099504 A1 WO 2022099504A1
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WIPO (PCT)
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electromagnetic
syringe
way valve
sampling needle
reagent
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PCT/CN2020/128107
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English (en)
Chinese (zh)
Inventor
王兴红
邹海涛
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深圳市科曼医疗设备有限公司
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Priority to PCT/CN2020/128107 priority Critical patent/WO2022099504A1/fr
Publication of WO2022099504A1 publication Critical patent/WO2022099504A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Definitions

  • the invention belongs to the technical field of medical blood detection, and in particular relates to a method for adding liquid for sampling and drawing reagents and a liquid circuit system thereof.
  • CRP blood routine
  • SAA SAA is a sensitive parameter. Rising, through the detection of both can be used as an important indicator to identify bacterial infection and viral infection, which is conducive to early diagnosis, effective treatment, lower mortality, while avoiding the abuse of antibiotics, reducing the use of drug-resistant bacteria, and improving the health of patients. Treatment is very important.
  • the method of adding the reagent to the syringe is used, as shown in Figure 1 of the specification, the syringe is connected to the reagent through the normally open end of the three-way valve, and the liquid is drawn when the syringe is pulled down; after the liquid is drawn, the three-way valve is reversed, and the three-way valve Switch to the normally closed end, connect it to the reaction tank, and add the reagent to the reaction tank under the thrust of the syringe to complete the liquid addition action.
  • This method is simple to operate, but when the reagents are replaced for the first time, all the pipelines need to be replaced, and the old reagents are replaced with new reagents, which is a serious waste.
  • the test results of the first few groups of the test will be unstable.
  • the liquid addition pipeline needs to extend into the reaction tank in this way, so in order to prevent the reagent and the reaction sample from contacting the front end of the reagent pipeline (at O) Usually, a section of isolation bubble is sucked, and the operation is cumbersome.
  • the method of adding reagents by a quantitative pump can save costs without using a syringe.
  • a mid-to-high-end analyzer with a gas circuit system the specific process is as follows: under normal conditions, the negative pressure is connected to the quantitative pump, and is connected to the reagent through the normally open end of the three-way valve, so the reagent pipeline has been filled with the reagent under normal conditions. Then switch the three-way valve to connect the quantitative pump with the positive pressure.
  • the diaphragm of the quantitative pump Under the power of the positive pressure, the diaphragm of the quantitative pump is restored, and the corresponding volume of reagent is pushed out to the reaction tank to complete the liquid addition process; this method is different from the use of a syringe.
  • the method of adding reagents also has the problem of refrigeration, which leads to waste of reagents.
  • the quantitative pump used as the name suggests, the reagents added each time are quantitative, so it is inconvenient to change the dosage of reagents, and it is not very convenient in the early stage of research and development.
  • the present invention provides a method for sampling, drawing, and adding reagents, comprising: running a sampling needle of a liquid circuit system to a designated position of a reagent bottle; obtaining the reagent in the reagent bottle and filling the reagent through a pull-down stroke of a fourth syringe In the sampling needle and the communication pipe connected between the sampling needle and the third syringe; the sampling needle goes up, the first syringe or the second syringe sucks the diluent and then discharges the diluent to the sampler set in the sampling needle.
  • the sampling needle runs into the reaction tank, and the reagent is discharged into the reaction tank through the push-up stroke of the fourth syringe; through the first syringe or the second syringe
  • the syringe or the fourth syringe obtains the diluent, and discharges the diluent through the third syringe into the sampling needle and the communication pipe until it is confluent into the swab for recovery.
  • the invention provides a liquid circuit system for sampling and absorbing reagents, comprising: a first syringe, a third syringe, a fourth syringe, a sampling needle, a swab disposed outside the sampling needle, a reagent bottle containing reagents, a Dilution device with diluent, reaction tank, connecting pipe, first electromagnetic three-way valve, second electromagnetic three-way valve, third electromagnetic three-way valve, fourth electromagnetic three-way valve, fifth electromagnetic three-way valve, third electromagnetic three-way valve A joint and a second joint; wherein, the output end of the fourth syringe is connected and communicated with the COM end of the first electromagnetic three-way valve through the communication pipe, and the NO end of the first electromagnetic three-way valve passes through The communication pipe is connected and communicated with the dilution device, the NC end of the first electromagnetic three-way valve is connected to the interior of the third syringe through the communication pipe and the first joint, and the third sy
  • the output ends of the three syringes are connected and communicated with one end of the sampling needle through the communication pipe, and are used for the fourth syringe to obtain the reagent and put the reagent into the reaction tank; wherein, the swab A port and a B port are arranged on one side from top to bottom in sequence, the output end of the first syringe is connected and communicated with the COM end of the second electromagnetic three-way valve through the communication pipe, and the second electromagnetic
  • the NO end of the three-way valve is connected and communicated with the dilution device through the communication pipe and the second joint, and the NC end of the second electromagnetic three-way valve is connected to the third electromagnetic three-way through the communication pipe
  • the COM end of the valve is connected and communicated, the NO end of the third electromagnetic three-way valve is connected and communicated with the NO end of the fourth electromagnetic three-way valve through the communication pipe, and the COM end of the fourth electromagnetic three-way valve is connected and communicated.
  • the terminal is connected to and communicated with the COM end of the fifth electromagnetic three-way valve through the communication pipe, and the NO end of the fifth electromagnetic three-way valve is connected to the B port of the swab through the communication pipe and is connected to the port B of the swab. It is used for the first syringe to suck the diluent and discharge the diluent into the swab set on the outside of the sampling needle to complete the cleaning of the outer wall of the sampling needle and the diluent. recycling.
  • the sampling needle is directly inserted into the reagent bottle to absorb the reagent of the required volume for the reaction, and then quickly added to the reaction tank, there will be no problem of reagent storage or excessive reagent waste caused by pipelines; simplifying the requirements for the reaction tank structure, no problem There is a problem of mutual contamination caused by the contact between the reagent and the reaction sample during the reaction process; it is not necessary to absorb the isolation bubbles to simplify the operation; the existing syringe types are rationally used, and different syringe specifications are used according to the required amount of reagents, so as to improve the utilization rate of the device components.
  • the height of the needle under the sampling needle can be controlled according to the amount of reagent in the reagent bottle through the sequence command, and the contact area between the sampling needle and the reagent can be reduced. Reduce the difficulty of cleaning the outer wall, save cleaning reagents, and improve cleaning efficiency.
  • Fig. 1 is the schematic diagram of the method that the prior art provided by the present embodiment adopts the syringe to add reagents;
  • Fig. 2 is the schematic diagram of the method that the prior art provided by the present embodiment adopts a quantitative pump to add reagents
  • Fig. 3 is the flow chart of a kind of sampling drawing reagent adding liquid method provided by the present embodiment
  • FIG. 4 is a schematic diagram of the sampling needle running steps provided by the present embodiment
  • Fig. 5 is the read-write framework diagram of the configuration file provided by the present embodiment
  • FIG. 6 is a schematic structural diagram of a sampling and drawing reagent liquid circuit system provided in this embodiment.
  • the sampling needle runs to the top of the reagent bottle through a horizontal motor and runs to the designated position of the reagent bottle through a vertical motor; the vertical motor is controlled by a sequence command to drive the sampling needle from the designated position.
  • the step sequence of the liquid level of the reagent in the reagent bottle is S
  • the step sequence of moving the sampling needle from the liquid surface to the bottom of the reagent bottle is set to be H, then: when I ⁇ N When /8, the number of steps that the sampling needle runs downward is S+H/4; when N/8 ⁇ I ⁇ N*3/8, the number of steps that the sampling needle runs downward is S+H/ 4; When N*3/8 ⁇ I ⁇ N*5/8, the number of steps that the sampling needle runs downward is S+H*3/4; when N*5/8 ⁇ I ⁇ N,
  • Segment the running steps of the sampling needle in combination with the suction times of the reagent set a step table, write the step table into a configuration file, and drive the sampling needle by the vertical motor by the specified number of steps. Before the position drops, read the configuration file.
  • It includes a second syringe, a third joint and a sixth electromagnetic three-way valve, the second syringe is connected and communicated with the COM end of the sixth electromagnetic three-way valve through the communication pipe and the third joint, and the The NO end of the sixth electromagnetic three-way valve is connected and communicated with the dilution device through the communication pipe and the second joint, and the NC end of the fourth electromagnetic three-way valve is connected to the third electromagnetic three-way valve through the communication pipe.
  • the joint is connected and communicated, and is used for cleaning the outer wall or the inner wall of the sampling needle after the second syringe absorbs the diluent.
  • the NC end of the fifth electromagnetic three-way valve is connected to the inside of the third syringe through the communication pipe and the first joint, and is used for cleaning the sampling needle after the first syringe absorbs the diluent. the inner wall.
  • It also includes a waste liquid device for recovering the diluent, the A port is connected and communicated with the waste liquid device through the communication pipe, and the waste liquid device is in a negative pressure environment.
  • It also includes a seventh electromagnetic three-way valve, the seventh electromagnetic three-way valve is arranged between the fourth syringe and the first electromagnetic three-way valve, and the output end of the fourth syringe is connected to the The COM end of the seventh electromagnetic three-way valve is connected and communicated, the NO end of the seventh electromagnetic three-way valve is connected and communicated with the COM end of the first electromagnetic three-way valve, and the seventh electromagnetic three-way valve is connected and communicated.
  • the NC end is connected and communicated with the bottom of the reaction tank through the communication pipe, and is used for forming a vortex flow between the sample in the reaction tank and the reagent or sucking the diluent to flush and clean the reaction tank.
  • the volume of the first syringe and the second syringe is 10 mL
  • the volume of the third syringe is 100 uL
  • the volume of the fourth syringe is 2.5 mL.
  • the NC end of the third electromagnetic three-way valve is connected with the impedance module through the communication pipe, and the NC end of the sixth electromagnetic three-way valve is connected with the sheath flow module through the communication pipe.
  • Step S101 the sampling needle of the liquid circuit system runs to the designated position of the reagent bottle.
  • the sampling needle is one of the components of the sampling assembly, and the sampling assembly also includes a horizontal motor and a vertical motor, which are used to rapidly move the sampling needle to a commanded position in a step-by-step manner.
  • sampling needle runs to the proper position of the reagent bottle, it also includes: the sampling needle runs to the top of the reagent bottle through the horizontal motor and runs to the designated position of the reagent bottle through the vertical motor. It should be noted that , the designated position refers to the position where the sampling needle is directly above the reagent bottle and not in contact with the reagent;
  • the number of steps in the whole process of the downward running of the sampling needle is a dynamic quantity.
  • the setting is represented by 999 characters.
  • the host reads 999 characters, it is considered that It is a dynamic variable, so as to query the configuration file and read the correct number of steps, please refer to Table 2.
  • Table 2 indicates the timing parameter command: the start time is the time when the action starts to execute, which is determined by the actual action, here is the first 1.38s ,;
  • the motor is a vertical motor, code MOT_02; the speed is 1084 steps/s; the number of steps takes into account the dynamic parameters, and the specific number of steps varies with the amount of reagent in the reagent bottle, so the time sequence is 999; time represents this action
  • the time required for running, in order to simplify the timing variables, the time here is the time from the sampling component to the longest distance from the highest height to the bottom of the bottle, the specific time is 1s, to ensure that the sampling component runs to any position of the reagent bottle The time is sufficient of.
  • Step S102 through the pull-down stroke of the fourth syringe, the reagent in the reagent bottle is obtained and the reagent is filled in the sampling needle and the communication tube connected between the sampling needle and the third syringe.
  • step S103 the sampling needle moves upward, and the first syringe or the second syringe sucks the diluent and discharges the diluent into a swab disposed outside the sampling needle for recovery.
  • the cleaning of the outer wall of the sampling needle is completed through the discharge of the diluent by the first syringe or the second syringe.
  • step S104 the sampling needle runs into the reaction tank, and the reagent is discharged into the reaction tank through the upward stroke of the fourth syringe.
  • the first syringe or the second syringe can continue to repeat the step of drawing the diluent and discharging the diluent to the swab set outside the sampling needle for recovery. Clean the outer wall of the sampling needle.
  • step S105 the diluent is obtained through the first syringe, the second syringe or the fourth syringe, and the diluent is discharged into the sampling needle and the communication tube through the interior of the third syringe until it converges into the swab for recovery.
  • the cleaning of the inner wall of the sampling needle is completed by discharging the diluent into the sampling needle and the communication pipe through the interior of the third syringe.
  • the fourth syringe acts on the reaction cell through its push-up stroke or pull-down stroke, so as to make the samples and reagents in the reaction cell form a vortex flow or absorb diluent to flush and clean the reaction cell.
  • an embodiment of the present invention provides a system for sampling and absorbing reagents and adding liquid, as shown in FIG. 6 , including: a first syringe 1 , a third syringe 2 , a fourth syringe 3 , a sampling needle 4 , a 4 The swab on the outside 5, the reagent bottle 6 containing the reagent, the dilution device 7 containing the diluent, the reaction tank 8, the communication pipe 9, the first electromagnetic three-way valve 10, the second electromagnetic three-way valve 11, The third electromagnetic three-way valve 12 , the fourth electromagnetic three-way valve 13 , the fifth electromagnetic three-way valve 14 , the first joint 15 and the second joint 16 ; wherein, the output end of the fourth syringe 3 is connected to the first The COM end of the electromagnetic three-way valve 10 is connected and communicated, the NO end of the first electromagnetic three-way valve 10 is connected and communicated with the dilution device 7 through the communication pipe 9, and the
  • a joint 15 is connected and communicated with the interior of the third syringe 2, and the output end of the third syringe 2 is connected and communicated with one end of the sampling needle 4 through the communication pipe 9, for the fourth syringe 3 to obtain the reagent and put the reagent into the reaction tank 8; wherein, one side of the swab 5 is sequentially provided with an A port 501 and a B port 502 from top to bottom, and the output end of the first syringe 1 is connected with the COM end of the second electromagnetic three-way valve 11 through the communication pipe 9 and The NO end of the second electromagnetic three-way valve 11 is connected and communicated with the dilution device 7 through the communication pipe 9 and the second joint 16, and the NC end of the second electromagnetic three-way valve 11 is connected with the third electromagnetic three-way valve through the communication pipe 9.
  • the COM end of 12 is connected and communicated, the NO end of the third electromagnetic three-way valve 12 is connected and communicated with the NO end of the fourth electromagnetic three-way valve 13 through the communication pipe 9, and the COM end of the fourth electromagnetic three-way valve 13 is connected and communicated through the communication pipe.
  • the diluent is discharged into the swab 5 provided on the outside of the sampling needle 4 to complete the cleaning of the outer wall of the sampling needle 4 and the recovery of the diluent.
  • the process includes a second syringe 17 , a third joint 18 and a sixth electromagnetic three-way valve 19 .
  • the second syringe 17 is connected to the COM end of the sixth electromagnetic three-way valve 19 through the communication pipe 9 and the third joint 18
  • the NO end of the sixth electromagnetic three-way valve 19 is connected and communicated with the dilution device 7 through the communication pipe 9 and the second joint 16, and the NC end of the fourth electromagnetic three-way valve 13 is connected with the third joint 18 through the communication pipe 9. and communicate with each other for cleaning the outer wall or inner wall of the sampling needle after the second syringe 17 sucks the diluent.
  • the NC end of the fifth electromagnetic three-way valve 14 is connected to the inside of the third syringe 2 through the communication pipe 9 and the first joint 15, and is used for cleaning the inner wall of the sampling needle after the first syringe 1 absorbs the diluent.
  • the waste liquid device 20 for recovering the diluent is also included.
  • the A port 501 is connected and communicated with the waste liquid device 20 through the communication pipe 9, and the waste liquid device 20 is in a negative pressure environment.
  • a seventh electromagnetic three-way valve 21 is also included.
  • the seventh electromagnetic three-way valve 21 is arranged between the fourth syringe 3 and the first electromagnetic three-way valve 10 , and the output end of the fourth syringe 3 is connected to the The COM end of the seventh electromagnetic three-way valve 21 is connected and communicated, the NO end of the seventh electromagnetic three-way valve 21 is connected and communicated with the COM end of the first electromagnetic three-way valve 10, and the NC end of the seventh electromagnetic three-way valve 21 passes through
  • the communication pipe 9 is connected and communicated with the bottom of the reaction cell 8 , and is used for forming a vortex flow between the sample and the reagent in the reaction cell 8 or drawing a diluent to wash and clean the reaction cell 8 .
  • the volumes of the first syringe 1 and the second syringe 17 are 10 mL, the volume of the third syringe 2 is 100 uL, and the volume of the fourth syringe 3 is 2.5 mL.
  • the NC end of the third electromagnetic three-way valve 12 is connected through the communication pipe 9 and communicated with the impedance module 22
  • the NC end of the sixth electromagnetic three-way valve 19 is connected through the communication pipe 9 and communicated with the sheath flow module 23 .
  • the first joint 15 , the second joint 16 and the third joint 18 are all three-way joints.
  • the sampling needle 4 of the sampling assembly runs to the top of the reagent bottle 6 through the horizontal motor and runs downward into the reagent bottle 6 through the vertical motor.
  • the COM end is connected with its NC end, and the fourth syringe 3 is communicated with the third syringe 2 and the sampling needle 4 through the communication pipe 9, the seventh electromagnetic three-way valve 21, the first electromagnetic three-way valve 10 and the first joint 15.
  • the four syringes 3 pull down the stroke to extract the required amount of reagent, thereby sucking the reagent of the corresponding volume from the reagent bottle 6, and the sucked reagent is stored in the sampling needle 4 and the communication pipe 9 connected between the sampling needle 4 and the third syringe 2;
  • the sampling needle 4 After sucking the reagent, the sampling needle 4 goes up to the top of the reagent bottle 6. Since the outer wall of the sampling needle 4 is in contact with the reagent during the sampling process, in order to avoid the influence on the action of the next round, it is necessary to clean the outer wall of the sampling needle 4, that is, the first syringe 1 is connected by The pipe 9, the second electromagnetic three-way valve 11 and the second joint 16 extract the diluent from the diluting device 7, the second electromagnetic three-way valve 11 is energized and reversed, and the COM end of the second electromagnetic three-way valve 11 is connected to its NC end.
  • the first syringe 1 passes through the communication pipe 9, the second electromagnetic three-way valve 11, the third electromagnetic three-way valve 12, the fourth electromagnetic three-way valve 13 and the fifth electromagnetic three-way valve 14 to the B port 502 of the swab 5
  • the diluent is discharged inside. Under the action of the inner cavity and liquid flow of the swab 5, the outer wall of the sampling needle 4 is cleaned. in the waste liquid device 20 to complete the cleaning and recycling operations;
  • the second syringe 17 can also complete the process of cleaning the outer wall of the sampling needle 4. Specifically, the second syringe 17 passes through the communication pipe 9, the third joint 18, and the sixth electromagnetic three-way valve.
  • the sixth electromagnetic three-way valve 19 and the fourth electromagnetic three-way valve 13 extract the diluent from the dilution device 7, the sixth electromagnetic three-way valve 19 and the fourth electromagnetic three-way valve 13 are powered on and reversed, and the COM end of the sixth electromagnetic three-way valve 19 is disconnected from its NO end , the NC end of the fourth electromagnetic three-way valve 13 is connected to its COM end, and the second syringe 17 sends the swab 5 to the swab 5 through the communication pipe 9, the third joint 18, the fourth electromagnetic three-way valve 13 and the fifth electromagnetic three-way valve 14.
  • the diluent is discharged in the B port 502 of the swab 5.
  • the sampling needle 4 runs into the reaction cell 8 through the horizontal motor and the vertical motor, the first electromagnetic three-way valve 10 is powered on and reversed, and the fourth syringe 3 pushes up the stroke, under the action of the thrust of the syringe. , the reagents stored in the sampling needle 4 and the communication pipe 9 connected between the sampling needle 4 and the third syringe 2 are discharged into the reaction tank 8, and the process of adding liquid to the reaction tank 8 is completed;
  • the sampling needle 4 goes up, and the above-mentioned steps of cleaning the outer wall of the sampling needle 4 are repeated;
  • the communication pipe 9 between the The valve 10 is powered on and reversed, the COM end of the first electromagnetic three-way valve 10 is connected to its NC end, and the fourth syringe 3 passes through the communication pipe 9, the seventh electromagnetic three-way valve 21, the first electromagnetic three-way valve 10 and the first electromagnetic three-way valve 10.
  • the joint 15 is communicated with the third syringe 2 and the sampling needle 4, the fourth syringe 3 removes the diluent, and the diluent flows through the communication pipe 9 and the sampling needle 4 connected between the sampling needle 4 and the third syringe 2, and the internal cleaning is completed.
  • the cleaning strength can be controlled by the gear position of the syringe, and the cleaning degree can be controlled by controlling the diluent discharged from the syringe.
  • the cleaned diluent is recovered in the swab 5 and discharged to the waste liquid device 20 through the A port 501 in order to complete the cleaning and recycling operations;
  • first syringe 1 can also complete the cleaning of the inner wall of the sampling needle 4 and the communication tube 9 connected between the sampling needle 4 and the third syringe 2. Specifically, the first syringe 1 can pass the communication tube 9, the second electromagnetic The three-way valve 11 and the second joint 16 extract the diluent from the dilution device 7 .
  • the second electromagnetic three-way valve 11 and the fifth electromagnetic three-way valve 14 are powered on and reversed, and the COM of the second electromagnetic three-way valve 11
  • the terminal is connected to its NC end
  • the COM end of the fifth electromagnetic three-way valve 14 is connected to its NC end
  • the first syringe 1 passes through the communication pipe 9, the second electromagnetic three-way valve 11, the third electromagnetic three-way valve 12, the fourth electromagnetic three-way valve 12, and the fourth electromagnetic three-way valve.
  • the electromagnetic three-way valve 13 , the fifth electromagnetic three-way valve 14 and the first joint 15 communicate with the interior of the third syringe 2 , the first syringe 1 discharges the diluent, and the diluent is connected to the sampling needle 4 and the sampling needle 4 through the interior of the third syringe 2 .
  • the communication tube 9 between the third syringes 2 and the inner wall of the sampling needle 4 are cleaned, and the diluted solution after cleaning is recovered in the swab 5 and discharged into the waste liquid device 20 through the A port 501 to complete the cleaning and recovery operations. ;
  • the second syringe 17 can also complete the cleaning of the inner wall of the sampling needle 4 and the communication pipe 9 connected between the sampling needle 4 and the third syringe 2.
  • the sixth electromagnetic The through valve 19 , the fourth electromagnetic three-way valve 13 and the fifth electromagnetic three-way valve 14 are powered on and reversed, that is, the second syringe 17 passes through the communication pipe 9 , the third joint 18 , the fourth electromagnetic three-way valve 13 , and the fifth electromagnetic three-way valve 13 .
  • the three-way valve 14 and the first joint 15 communicate with the interior of the third syringe 2 , and the diluent cleans the communication tube 9 connected between the sampling needle 4 and the third syringe 2 and the inner wall of the sampling needle 4 through the interior of the third syringe 2 , the diluted solution after cleaning is recovered in the swab 5, and discharged into the waste liquid device 20 through the A port 501, so as to complete the cleaning and recovery operations.
  • the fourth syringe 3 is connected to the bottom of the reaction tank 8 through the communication pipe 9 and the seventh electromagnetic three-way valve 21.
  • the samples and reagents in the reaction tank 8 form a vortex flow, and the mixing of the samples and reagents in the reaction tank 8 is completed.
  • the cleaning of the reaction tank 8 is also completed by the fourth syringe 3.
  • the fourth syringe 3 absorbs and dilutes After the liquid is discharged, the seventh electromagnetic three-way valve 21 is powered on and reversed, the COM terminal of the seventh electromagnetic three-way valve 21 is connected to its NC terminal, and the fourth syringe 3 discharges the diluent into the reaction tank 8 for flushing and cleaning.
  • SAA and CRP latex reagents are used as examples, which generally require 200-300ul in the detection items, so a 2.5ml fourth syringe 3 is used to absorb and discharge the reagents. If it is the fluorescence of RET For reagents, which only need 20ul for one measurement, the third syringe 2 of 100ul can be used. For some projects that require more than 1ml of reagent, the first syringe 1 or the second syringe 17 of 10mL can be used as the power to suck the reagent, so This system is suitable for a variety of reagent specifications, effectively ensuring the precision of the aspirating reagents.
  • the diluent in addition to the above-mentioned cleaning of the inner and outer walls of the sampling needle 4, the diluent also needs to be provided for the impedance module 22 (for detecting red blood cells, packet cells, hgb, and platelet parameters);
  • the impedance module 22 for detecting red blood cells, packet cells, hgb, and platelet parameters
  • sheath fluid to the sheath flow module 23 (used to classify leukocytes into five categories); therefore, the liquid addition system can reasonably arrange the use of the device parts according to the actual use of the device parts, reducing the waiting time and avoiding Action conflict, improve measurement speed.
  • the present sampling and drawing reagent adding system has the following beneficial effects: the sampling needle 4 directly extends into the reagent bottle 6 to absorb the reagent of the required volume for the reaction, and then quickly adds it to the reaction tank 8, so that there will be no problem of reagent storage or reagent storage.
  • Excessive pipelines cause waste; simplify the requirements for the structure of the reaction tank 8, and there will be no mutual contamination caused by the contact between reagents and reaction samples during the reaction process; it is not necessary to absorb isolation bubbles, simplifying the operation; rational use of existing Different types of syringes are used according to the required amount of reagents, which improves the utilization rate of parts of the device and reduces the waiting time for action.
  • the sequence command can be used, according to the 6 in the reagent bottle.
  • the amount of reagent already used, the height of the lower needle of the sampling needle 4 is controlled, the contact area between the sampling needle 4 and the reagent is reduced, the difficulty of cleaning the outer wall is reduced, the cleaning reagent is saved, and the cleaning efficiency is improved.
  • the present application has industrial applicability.

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Abstract

L'invention concerne un procédé d'ajout de liquide pour échantillonnage et prélèvement d'un réactif, et un système de trajet de liquide pour celui-ci. Le procédé comprend : le déplacement d'une aiguille d'échantillonnage d'un système de trajet de liquide vers une position spécifiée d'une bouteille de réactif; au moyen d'une course d'abaissement d'un quatrième injecteur, l'acquisition d'un réactif dans la bouteille de réactif, et le fait de permettre le remplissage par le réactif de l'aiguille d'échantillonnage et d'un tuyau de communication raccordés entre l'aiguille d'échantillonnage et un troisième injecteur; l'ascension de l'aiguille d'échantillonnage, et le prélèvement par un premier injecteur ou un second injecteur d'un diluant, puis la décharge du diluant dans un tampon disposé sur le côté extérieur de l'aiguille d'échantillonnage pour le recyclage; le déplacement de l'aiguille d'échantillonnage dans un réservoir de réaction, et la décharge du réactif dans le réservoir de réaction au moyen d'une course de poussée du quatrième injecteur; et l'acquisition du diluant par l'intermédiaire du premier injecteur ou du deuxième injecteur ou du quatrième injecteur, et la décharge du diluant dans l'aiguille d'échantillonnage et le tuyau de communication au moyen de l'intérieur du troisième injecteur jusqu'à ce que le diluant converge dans le tampon pour le recyclage. Le système de trajet de liquide comprend un premier injecteur, un troisième injecteur, un quatrième injecteur, une aiguille d'échantillonnage, un tampon disposé sur le côté extérieur de l'aiguille d'échantillonnage, une bouteille de réactif qui contient un réactif, un dispositif de dilution qui contient un diluant, un réservoir de réaction, un tuyau de raccordement, une première vanne électromagnétique à trois voies, une deuxième vanne électromagnétique à trois voies, une troisième vanne électromagnétique à trois voies, une quatrième vanne électromagnétique à trois voies, une cinquième vanne électromagnétique à trois voies, un premier joint et un deuxième joint. L'utilisation du procédé dans la présente invention permet de garantir l'absence de gaspillage de réactif, l'absence de contamination des liquides ajoutés, la rapidité, l'efficacité et la précision de l'ajout de liquide, une uniformité de mélange suffisante, une amélioration de l'efficacité, et l'amélioration de la compétitivité sur le marché.
PCT/CN2020/128107 2020-11-11 2020-11-11 Procédé d'ajout de liquide pour échantillonnage et prélèvement de réactif, et système de trajet de liquide correspondant WO2022099504A1 (fr)

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