WO2022099343A1 - Solid support comprising a set of protein arrays - Google Patents

Solid support comprising a set of protein arrays Download PDF

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Publication number
WO2022099343A1
WO2022099343A1 PCT/AT2021/060428 AT2021060428W WO2022099343A1 WO 2022099343 A1 WO2022099343 A1 WO 2022099343A1 AT 2021060428 W AT2021060428 W AT 2021060428W WO 2022099343 A1 WO2022099343 A1 WO 2022099343A1
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Prior art keywords
tri
der
amb
asp
pru
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PCT/AT2021/060428
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French (fr)
Inventor
Huey-Jy HUANG
Katarzyna Niespodziana
Susanne Vrtala
Rudolf Valenta
Thomas Schlederer
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Hvd Biotech Vertriebsgesellschaft M.B.H.
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Publication of WO2022099343A1 publication Critical patent/WO2022099343A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/24—Immunology or allergic disorders

Definitions

  • the present invention relates to the field of diagnostics, in particular of allergy diagnostics.
  • protein and peptide arrays are typically prepared directly on substrates which are thereafter used to perform specific analytical methods. These substrates have or are usually embedded in a predetermined architecture. A specific architecture is typically required to fit into respective analytical devices. Examples of such disadvantageous chips and devices include ImmunoCAP ISAC from Thermofisher, the MeDALL allergen chip and the macro-array ALEX (Heffler E et al. World Allergy Organ J (2016) 11 (1) :7) , whereby ImmunoCAP ISAC and MeDALL contain several arrays whereas macroarray ALEX contains only one.
  • the present invention relates to a solid support comprising a set of protein and/or nucleic acid peptide arrays, wherein said arrays are surrounded by a circular predetermined breaking point defining a solid support element.
  • the solid support of the present invention comprises one or more, preferably at least two, protein and/or peptide arrays, wherein each of these arrays is separated by one or more breaking points surrounding these arrays.
  • the breaking points circularly surrounding the arrays are required to break out elements, "solid support elements, from the solid support which comprise on their surface the protein and/or peptide arrays.
  • These solid support elements can be positioned on respective carriers which thereafter can be used for analytical purposes.
  • solid support elements are arranged in much shorter distance close to each other for the spotting than preformed on chips, for instance. Accordingly the spotting machine (e.g. a microarray printer) makes much shorter movements when spotting closely assembled solid support elements as compared to premade chips which should speed up the production by shortening the production time.
  • a microarray printer e.g. a microarray printer
  • a solid support of the present invention comprising a set of protein and/or peptide arrays helps to keep loss of material low, since single elements of the solid support, which may be defective, can be removed without the need to discard a whole device, e.g. chip, comprising arrays on their surface.
  • the solid support of the present invention comprising single solid support elements
  • the single elements can be assembled in different formats for different uses. This allows, for instance, producing devices containing only one type of array for fast testing of single samples or devices comprising several different arrays on solid support elements for testing different samples.
  • Typical devices that may comprise solid support elements include plates (e.g., ELISA plate format) for automated processing of samples or chips.
  • arrays printed on solid support elements allow assembling of different devices for testing based on one standardized element.
  • Another aspect of the present invention relates to a method for producing a device comprising a protein and/or peptide array comprising the steps of
  • the solid support of the present invention can be used to produce devices comprising solid support elements broken out of the solid support. These elements can be assembled/mounted to/on devices which are then used to analyse samples.
  • Fig. 1 shows a chip comprising fixed arrays of proteins or peptides. Such chips are known in the art.
  • Fig. 2 shows a solid support element 1 broken out from a solid support 3 of the present invention.
  • the solid support element comprises an array of protein and/or peptide spots 2 immobilized/spotted on its surface .
  • Fig. 3 shows a solid support 3 comprising a set of arrays of protein and peptide spots 2. The arrays are surrounded by a circular predetermined breaking point defining a solid support element as shown in Fig. 2.
  • Fig. 4 shows a solid support (e.g. silicon wafer) comprising a multiplicity of protein and/or peptide arrays on its surface.
  • a solid support e.g. silicon wafer
  • One or more of the arrays can be broken or cut out and assembled on various platforms (e.g. chips or plates) .
  • the solid support of the present invention comprises proteins and/or peptides spotted thereon forming at least two, more preferably at least three, more preferably at least five, more preferably at least ten, arrays.
  • the arrays are separated from each other and surrounded by breaking points. These breaking points define solid support elements which can be broken out from the solid support .
  • a "protein array” and a “peptide array”, as defined herein, is a spatially defined arrangement of protein and peptide moieties, so called protein or peptide spots, in a pattern on a surface of a solid support of the present invention.
  • One type of protein or peptide forms one spot.
  • the protein and peptide moieties are attached to the surface of a solid support either directly or indirectly. The attachment can be non-specific (e.g. by physical absorption onto the surface or by formation of a non-specific covalent interaction) . Methods for immobilizing proteins and peptides on solid supports are well known in the art.
  • the number of protein and peptide spots attached on the solid support forming arrays of the invention will be determined to be sufficient for experimental, commercial or clinical interest and may vary from 1 to 10000, preferably 1 to 1000, more preferably 1 to 500, more preferably 1 to 400, more preferably 1 to 300, more preferably 1 to 200, more preferably 1 to 100, more preferably 1 to 75, more preferably 1 to 50.
  • the solid support of the present invention may comprise one or more protein and/or peptide arrays. It is particularly preferred that one array forms one solid support element which can be broken out of the solid support .
  • a "circular predetermined breaking point”, as defined herein, can be formed by various ways on the solid support provided that the application of a kind of force or stress on the solid support elements or the breaking point (s) results in the break.
  • the breaking point or points may be cut through the solid support leaving contacting points between the single solid support elements and/or the solid support.
  • Breaking points may be formed as a groove, e.g., by material removal by etching, laser, etc. so that the material thickness of the solid support at the breaking points is at least 50%, preferably at least 60%, more preferably at least 70%, more preferably at least 80%, more preferably at least 90%, more preferably at least 95%, reduced compared to the thickness of the solid support.
  • Circular predetermined breaking points may also be perforations surrounding the solid support elements of the solid support. The perforations may have any form (e.g. elongated, puncture) .
  • the circular predetermined breaking point is preferably an encircling weakness of the cross section, a groove, encircling perforations or a combination thereof.
  • the solid support comprises or consists of silicon, a plastic material or a metal.
  • the solid support may comprise or consist of various materials.
  • the solid support consists of silicon, wherein the solid support can be a silicon wafer. Silicon surfaces give 5- 10-fold higher sensitivity as compared to glass, for instance, allowing detecting also low allergen-specific antibody levels, e.g. IgE levels, with high precision and the measurement can be done with very simple and inexpensive detection devices.
  • the solid support may also comprise a metal surface (e.g. gold surface) .
  • the solid support may also consist of a body of one material, e.g. a plastic, comprising a modified surface or a metal (e.g. gold) surface.
  • the protein arrays on the solid support comprise at least two types of immobilized allergens or fragments thereof.
  • the allergens on the solid support may be full length allergens or mature allergens.
  • the solid support may also comprise fragments of allergens, wherein these fragments comprise preferably regions of allergens which comprise IgE binding motifs.
  • the allergens are selected from the group consisting of the at least two allergens are selected from the group consisting of Alpha Gal, Act d 1,
  • Act d 2 Act d 3, Act d 4, Act d 5, Act d 6, Act d 7, Act d 8, Act d 9, Act d 10, Act d 11, Act d 12, Act d 13, Aed a 1, Aed a 2, Aed a 3, Aed a 4, Aed a 5, Aed a 6, Aed a
  • Cyn d 4 Cyn d 5
  • Cyn d 6 Cyn d 7
  • Cyn d 11 Cyn d 12
  • Equ c 2 Equ c 3, Equ c 4, Equ c 6, Equ c 8, Equ c 9, Equ c 10, Equ c 11, Fag e 1, Fag e 2, Fag e 3, Fag e 4, Fag e
  • Pen c 1 Pen c 2
  • Pen c 3 Pen c 6
  • Pen c 13 Pen c 18,
  • Pen c 19 Pen c 22, Pen c 24, Pen c 30, Pen c 32, Pen m
  • Phi p Per a 2, Per a 3, Per a 4, Per a 5, Per a 6, Per a 7, Per a 8, Per a 9, Per a 10, Per a 11, Per a 12, Phi p 1, Phi p 2, Phi p 3, Phi p 4, Phi p 5, Phi p 5b, Phi p 6, Phi p
  • Pru p 3 Pru p 4, Pru p 7, Que a 1, Que a 2, Que a 4, Rat n 1, Rat n 4, Rat n 7, Rat n 8, Sal k 1, Sal k 2, Sal k
  • Tri a 30 Tri a 31, Tri a 32, Tri a 33, Tri a 34, Tri a 35, Tri a 36, Tri a 37, Tri a 39, Tri a 40, Tri a 41,
  • the solid support elements of the present invention can be assembled or mounted on devices which can be used for analytical methods thereafter.
  • the solid support elements are used to determine the presence or quantity of antibodies, in particular of IgA, IgE, IgG, IgGl, IgG2, IgG3, IgG4 and IgM immunoglobulins, binding to a an allergen or fragment thereof present in a sample (e.g. blood, plasma, serum, sputum) .
  • a sample e.g. blood, plasma, serum, sputum
  • the determination of the presence of these antibodies is useful in allergy diagnosis to determine whether an individual suffers from an allergy.
  • Another aspect of the present invention relates to a method for producing a device comprising at least one protein and/or peptide array comprising the steps of
  • One or more solid support elements can be broken or cut out of the solid support of the present invention comprising one or more, preferably more than two, protein and/or peptide arrays by applying force or using means for cutting (e.g. blade) . Thereafter, these solid support elements are mounted reversible or irreversible on devices which are used in analytical methods (e.g. chips, microarray ELISA plates or ELISA plate-like carriers) . Reversible mounting of the solid support elements is particularly advantageous because it allows to reuse the device on which the solid support elements have been mounted .
  • the solid support elements are mounted to the aforementioned device by using a glue, magnetic means or any other mechanical means to fix the solid support elements on the device.
  • Such fixing means are well known in the art .
  • the slides were incubated overnight in the dark in a 75 % humidity chamber to immobilize the allergens. After overnight incubation, the chips were sprayed with blocking buffer (30 mmol/L ethanolamine in PBS containing 0.1 % Tween-20) and incubated for 30 min at room temperature. After 30 min incubation, the slides were immersed in liquid plate sealer (CANDOR Bioscience GmbH, Germany) for 15 min. Slides are dried by centrifugation (200 g, 2 min) and stored under vacuum at 4 °C until use.
  • Fluorescence signals were detected by a TECAN Power Scanner (Austria) at 25% laser power and 50% photomultiplier (PMT) gain for IgE measurement and at 10% laser power and 10% PMT gain for IgG measurement. Fluorescence intensities are for example analyzed using Luxscan software (China) .
  • the buffer control i.e., result obtained only with buffer and detection antibodies
  • FI median fluorescence intensity
  • the cut-off values for IgE and IgG detection corresponding to 0.1 lU/mL were 100 and 10 fluorescence intensities (FIs) for IgE and IgG, respectively .

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • General Physics & Mathematics (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a solid support comprising a set of protein and/or peptide arrays for determining allergen-specific antibodies, wherein said arrays are surrounded by a circular predetermined breaking point defining a solid support element.

Description

SOLID SUPPORT COMPRISING A SET OF PROTEIN ARRAYS
TECHNICAL FIELD
[0001] The present invention relates to the field of diagnostics, in particular of allergy diagnostics.
BACKGROUND ART
[0002] Currently available protein and peptide arrays are manufactured in one predetermined format which is typically a chip containing one or several identically prepared protein and peptide arrays (see Fig. 1) which then need to be processed in the laboratory by hand pipetting. Disadvantages of known chips containing more than one array are that the spotting of the microarrays is performed directly on the chips which are relatively large. Examples of such disadvantageous chips and devices include ImmunoCAP ISAC from Thermofisher (Jakob T et al. Allergo J (2015) 24 (8) : 42-56) and the MeDALL allergen chip (Lupinek C et al. Methods (2014) 66(1) :106-119) .
[0003] Furthermore, protein and peptide arrays are typically prepared directly on substrates which are thereafter used to perform specific analytical methods. These substrates have or are usually embedded in a predetermined architecture. A specific architecture is typically required to fit into respective analytical devices. Examples of such disadvantageous chips and devices include ImmunoCAP ISAC from Thermofisher, the MeDALL allergen chip and the macro-array ALEX (Heffler E et al. World Allergy Organ J (2018) 11 (1) :7) , whereby ImmunoCAP ISAC and MeDALL contain several arrays whereas macroarray ALEX contains only one.
[0004] Consequently, depending on the equipment used, different substrates carrying protein and peptide arrays have to be used.
[0005] Another disadvantage of substrates containing several protein and peptide arrays is that one array of poor quality will lead to the discarding of a complete substrate (e.g. chip) although other arrays present on the substrate may meet the quality criteria.
[0006] It is an object of the present invention to provide a flexible system allowing to mount protein and peptide arrays on various substrates.
SUMMARY OF THE INVENTION
[0007] Hence, the present invention relates to a solid support comprising a set of protein and/or nucleic acid peptide arrays, wherein said arrays are surrounded by a circular predetermined breaking point defining a solid support element.
[0008] The solid support of the present invention comprises one or more, preferably at least two, protein and/or peptide arrays, wherein each of these arrays is separated by one or more breaking points surrounding these arrays. The breaking points circularly surrounding the arrays are required to break out elements, "solid support elements, from the solid support which comprise on their surface the protein and/or peptide arrays. These solid support elements can be positioned on respective carriers which thereafter can be used for analytical purposes.
[0009] One major advantage of solid support elements is that they can be arranged in much shorter distance close to each other for the spotting than preformed on chips, for instance. Accordingly the spotting machine (e.g. a microarray printer) makes much shorter movements when spotting closely assembled solid support elements as compared to premade chips which should speed up the production by shortening the production time.
[0010] Furthermore, a solid support of the present invention comprising a set of protein and/or peptide arrays helps to keep loss of material low, since single elements of the solid support, which may be defective, can be removed without the need to discard a whole device, e.g. chip, comprising arrays on their surface.
[0011] One of the most important advantages of the solid support of the present invention comprising single solid support elements is that after spotting of the arrays, the single elements can be assembled in different formats for different uses. This allows, for instance, producing devices containing only one type of array for fast testing of single samples or devices comprising several different arrays on solid support elements for testing different samples. Typical devices that may comprise solid support elements include plates (e.g., ELISA plate format) for automated processing of samples or chips. Thus, arrays printed on solid support elements allow assembling of different devices for testing based on one standardized element.
[0012] Another aspect of the present invention relates to a method for producing a device comprising a protein and/or peptide array comprising the steps of
- providing a solid support according to the present invention,
- breaking a circular predetermined breaking point of the solid support to remove a solid support element comprising a protein and/or peptide array and
- mounting said solid support element on a chip, microarray ELISA plate or ELISA plate-like carrier.
[0013] The solid support of the present invention can be used to produce devices comprising solid support elements broken out of the solid support. These elements can be assembled/mounted to/on devices which are then used to analyse samples.
BRIEF DESCRIPTION OF THE FIGURES
[0014] Fig. 1 shows a chip comprising fixed arrays of proteins or peptides. Such chips are known in the art.
[0015] Fig. 2 shows a solid support element 1 broken out from a solid support 3 of the present invention. The solid support element comprises an array of protein and/or peptide spots 2 immobilized/spotted on its surface .
[0016] Fig. 3 shows a solid support 3 comprising a set of arrays of protein and peptide spots 2. The arrays are surrounded by a circular predetermined breaking point defining a solid support element as shown in Fig. 2.
[0017] Fig. 4 shows a solid support (e.g. silicon wafer) comprising a multiplicity of protein and/or peptide arrays on its surface. One or more of the arrays can be broken or cut out and assembled on various platforms (e.g. chips or plates) .
DESCRIPTION OF EMBODIMENTS
[0018] The solid support of the present invention comprises proteins and/or peptides spotted thereon forming at least two, more preferably at least three, more preferably at least five, more preferably at least ten, arrays. The arrays are separated from each other and surrounded by breaking points. These breaking points define solid support elements which can be broken out from the solid support .
[0019] A "protein array" and a "peptide array", as defined herein, is a spatially defined arrangement of protein and peptide moieties, so called protein or peptide spots, in a pattern on a surface of a solid support of the present invention. One type of protein or peptide forms one spot. Preferably the protein and peptide moieties are attached to the surface of a solid support either directly or indirectly. The attachment can be non-specific (e.g. by physical absorption onto the surface or by formation of a non-specific covalent interaction) . Methods for immobilizing proteins and peptides on solid supports are well known in the art.
[0020] The number of protein and peptide spots attached on the solid support forming arrays of the invention will be determined to be sufficient for experimental, commercial or clinical interest and may vary from 1 to 10000, preferably 1 to 1000, more preferably 1 to 500, more preferably 1 to 400, more preferably 1 to 300, more preferably 1 to 200, more preferably 1 to 100, more preferably 1 to 75, more preferably 1 to 50. [0021] The solid support of the present invention may comprise one or more protein and/or peptide arrays. It is particularly preferred that one array forms one solid support element which can be broken out of the solid support .
[0022] A "circular predetermined breaking point", as defined herein, can be formed by various ways on the solid support provided that the application of a kind of force or stress on the solid support elements or the breaking point (s) results in the break. For instance, the breaking point or points may be cut through the solid support leaving contacting points between the single solid support elements and/or the solid support. Breaking points may be formed as a groove, e.g., by material removal by etching, laser, etc. so that the material thickness of the solid support at the breaking points is at least 50%, preferably at least 60%, more preferably at least 70%, more preferably at least 80%, more preferably at least 90%, more preferably at least 95%, reduced compared to the thickness of the solid support. Circular predetermined breaking points may also be perforations surrounding the solid support elements of the solid support. The perforations may have any form (e.g. elongated, puncture) .
[0023] Hence, the circular predetermined breaking point is preferably an encircling weakness of the cross section, a groove, encircling perforations or a combination thereof.
[0024] According to a preferred embodiment of the present invention the solid support comprises or consists of silicon, a plastic material or a metal.
[0025] The solid support may comprise or consist of various materials. In a particularly preferred embodiment the solid support consists of silicon, wherein the solid support can be a silicon wafer. Silicon surfaces give 5- 10-fold higher sensitivity as compared to glass, for instance, allowing detecting also low allergen-specific antibody levels, e.g. IgE levels, with high precision and the measurement can be done with very simple and inexpensive detection devices.
[0026] Depending on the use the solid support may also comprise a metal surface (e.g. gold surface) . The solid support may also consist of a body of one material, e.g. a plastic, comprising a modified surface or a metal (e.g. gold) surface.
[0027] According to preferred embodiment of the present invention the protein arrays on the solid support comprise at least two types of immobilized allergens or fragments thereof.
[0028] The allergens on the solid support may be full length allergens or mature allergens. The solid support may also comprise fragments of allergens, wherein these fragments comprise preferably regions of allergens which comprise IgE binding motifs.
[0029] According to another preferred embodiment of the present invention the allergens are selected from the group consisting of the at least two allergens are selected from the group consisting of Alpha Gal, Act d 1,
Act d 2, Act d 3, Act d 4, Act d 5, Act d 6, Act d 7, Act d 8, Act d 9, Act d 10, Act d 11, Act d 12, Act d 13, Aed a 1, Aed a 2, Aed a 3, Aed a 4, Aed a 5, Aed a 6, Aed a
7, Aed a 8, Aed a 10, Aed a 11, Ain g 1, Ain g 2, Ain g 4,
Alt a 1, Alt a 2, Alt a 3, Alt a 4, Alt a 5, Alt a 6, Alt a 7, Alt a 8, Alt a 9, Alt a 10, Alt a 12, Alt a 13, Alt a 14, Al t a 15, Amb a 1 , Amb a 1.0101, Amb a 1.0201, Amb a 1.0202, Amb a 1.0301, Amb a 1.0302, Amb a 1.0303, Amb a 1.0304, Amb a 1.0305, Amb a 1.0401, Amb a 1.0402, Amb a 1.0501, Amb a 1.0502, Amb a 3 , Amb a 4 , Amb a 5 , Amb a 6 , Amb a 7 , Amb a 8 , Amb a 9 , Amb a 10, Amb a 11, Amb a 12, Ana o 1, Ana o 2, Ana o 3, Ani s 1, Ani s 2, Ani s 3, Ani s 4, Ani s 5, Ani s 6, Ani s 7, Ani s 8, Ani s 9, Ani s 10, Ani s 11, Ani s 12, Ani s 13, Ani s 14, Api g 1, Api g 2, Api g 3, Api g 4, Api g 5, Api g 6, Api m 1, Api m
2, Api m 3, Api m 4, Api m 5, Api m 6, Api m 7, Api m 8,
Api m 9, Api m 10, Api m 11, Api m 12, Api m 13kD, Ara h
1, Ara h 2, Ara h 3, Ara h 5, Ara h 6, Ara h 7, Ara h 8, Ara h 9, Ara h 10, Ara h 11, Ara h 12, Ara h 13, Ara h 14, Ara h 15, Ara h 16, Ara h 17, Art v 1, Art v 2, Art v 3, Art v 4, Art v 5, Art v 6, Asp f 1, Asp f 2, Asp f 3,
Asp f 4, Asp f 5, Asp f 6, Asp f 7, Asp f 8, Asp f 9, Asp f 10, Asp f 11, Asp f 12, Asp f 13, Asp f 14, Asp f 15,
Asp f 16, Asp f 17, Asp f 18, Asp f 22, Asp f 23, Asp f
26, Asp f 27, Asp f 28, Asp f 29, Asp f 34, Ber e 1, Bet v 1, Bet v 2, Bet v 3, Bet v 4, Bet v 6, Bet v 7, Bet v
8, Bia g 1, Bia g 2, Bia g 3, Bia g 4, Bia g 5, Bia g 6,
Bia g 7, Bia g 8, Bia g 9, Bia g 10, Bia g 11, Bio t 1,
Bio t 2, Bio t 3, Bio t 4, Bio t 5, Bio t 6, Bio t 7, Bio t 8, Bio t 9, Bio t 10, Bio t 11, Bio t 12, Bio t 13, Bio t 14, Bio t 15, Bio t 18, Bio t 19, Bio t 20, Bio t 21,
Bos d 1, Bos d 2, Bos d 3, Bos d 4, Bos d 5, Bos d 6, Bos d 7, Bos d Lf, Bos d 8, Bos d 9, Bos d 11, Bos d 12, aSl, aS2, b-casein, k-casein, Transferrin, BSA, Bos d 6, Bra n 1, Bra n 4, Bra n 7, Bra n 8, Can f 1, Can f 2, Can f 3,
Can f 4, Can f 5, Can f 6, Can f 7, Can f 8, Cas s 1, Cas s 2, Cas s 5, Cas s 8, Cas s 9, Cha o 1, Cha o 2, Cha o
3, Che a 1, Cla h 1, Cla h 2, Cla h 5, Cla h 6, Cla h 7,
Cla h 8, Cla h 9, Cla h 10, Cla h 12, Cla h 13, Cor a 1,
Cor a 1.0401, Cor a 2, Cor a 6, Cor a 8, Cor a 9, Cor a
10, Cor a 11, Cor a 12, Cor a 13, Cor a 14, Cry j 1, Cry j 2, Cry j 3, Cry j 4, Cyn d 1, Cup a 1, Cup a 2, Cup a
3, Cup a 4, Cup s 1, Cup s 2, Cup s 3, Cyn d 1, Cyn d 2,
Cyn d 4, Cyn d 5, Cyn d 6, Cyn d 7, Cyn d 11, Cyn d 12,
Cyn d 13, Cyn d 15, Cyn d 22, Cyn d 23, Cyn d 24, Cyp c 1, Dau c 1, Dau c 3, Dau c 4, Dau c 5, Der p 1, Der f 1,
Der p 2, Der f 2, Der p 3, Der p 4, Der p 5, Der p 6, Der p 7, Der p 8, Der p 9, Der p 10, Der p 11, Der p 13, Der p 14, Der p 15, Der p 18, Der p 20, Der p 21, Der p 23,
Clone 16, Der p 24, Der p 36, Der p 37, Lep d 2, Equ c 1,
Equ c 2, Equ c 3, Equ c 4, Equ c 6, Equ c 8, Equ c 9, Equ c 10, Equ c 11, Fag e 1, Fag e 2, Fag e 3, Fag e 4, Fag e
5, Fag s 1, Fag s 2, Fag s 4, Eel d 1, Eel d 2, Eel d 3,
Eel d 4, Eel d 5, Eel d 6, Eel d 7, Eel d 8, Fra a 1, Fra a 3, Fra a 4, Fra e 1, Fra e 2, Fra e 3, Fra e 6, Fra e
7, Fra e 9, Fra e 10, Fra e 11, Fra e 12, Gad c 1, Gad m 1, Gad m 2, Gad m 3, Gad m 4, Gal d 1, Gal d 2, Gal d 3, Gal d 4, Gal d 5, Gal d 6 f Gal d 7, Gal d 8, Gal d 9, Gal d 10, Gly m 1, Gly m 2, Gly m 3, Gly m 4, Gly m 5, Gly m
6, Gly m 7, Gly m 8, Hel a 1, Hel a 2, Hel a 3, Hel a 4,
Hel a 6, Hev b 1, Hev b 2, Hev b 3, Hev b 4, Hev b 5, Hev b 6, Hev b 6.01, Hev b 7, Hev b 8, Hev b 9, Hev b 10, Hev b 11, Hev b 12, Hev b 13, Hev b 14, Hev b 15, Hom a 1, Hom a 3, Hom a 4, Hom a 6, Hum j 1, Hum j 2, Hum j 3, Jug n 1, Jug n 2, Jug n 4, Jug r 1, Jug r 2, Jug r 3, Jug r
4, Jug r 5, Jug r 6, Jug r 7, Jug r 8, Jun a 1, Jun a 2,
Jun a 3, Len c 1, Len c 2, Len c 3, Lep d 2, Lep d 3, Lep d 5, Lep d 7, Lep d 8, Lep d 10, Lep d 12, Lep d 13, Lep d 33, Mai d 1, Mai d 2, Mai d 3, Mai d 4, Mala s 1, Mala s 4, Mala s 5, Mala s 6, Mala s 7, Mala s 8, Mala s 9,
Mala s 10, Mala s 11, Mala s 12, Mala s 13, Mer a 1, Mus m 1, Mus m 2, Mus m 4, Mus m 7, MUXF3, Ole e 1, Ole e 2, Ole e 3, Ole e 4, Ole e 5, Ole e 6, Ole e 7, Ole e 8, Ole e 9, Ole e 10, Ole e 11, Ole e 12, Ole e 13, Ole e 14,
Ole e 15, Ory c 1, Ory c 3, Ory c 4, Ory c 6, Ory c 8, Ory s 1, Ory s 2, Ory s 3, Ory s 7, Ory s 11, Ory s 12,
Ory s 13, Ory s 14, Par j 1, Par j 2, Par j 3, Par j 4,
Pen c 1, Pen c 2, Pen c 3, Pen c 6, Pen c 13, Pen c 18,
Pen c 19, Pen c 22, Pen c 24, Pen c 30, Pen c 32, Pen m
1, Pen m 2, Pen m 3, Pen m 4, Pen m 6, Pen m 8, Per a 1,
Per a 2, Per a 3, Per a 4, Per a 5, Per a 6, Per a 7, Per a 8, Per a 9, Per a 10, Per a 11, Per a 12, Phi p 1, Phi p 2, Phi p 3, Phi p 4, Phi p 5, Phi p 5b, Phi p 6, Phi p
7, Phi p 11, Phi p 12, Phi p 13, Pis s 1, Pis s 2, Pis s
3, Pis s 5, Pis s 6, Pis v 3, Pla a 1, Pla a 2, Pla a 3,
Pla a 8, Pla 1 1, Pol d 5, Pru av 1, Pru av 2, Pru av 3, Pru av 4, Pru du 1, Pru du 2, Pru du 3, Pru du 4, Pru du
5, Pru du 6, Pru du 6.01, Pru du 6.02, Pru p 1, Pru p 2,
Pru p 3, Pru p 4, Pru p 7, Que a 1, Que a 2, Que a 4, Rat n 1, Rat n 4, Rat n 7, Rat n 8, Sal k 1, Sal k 2, Sal k
3, Sal k 4, Sal k 5, Sal k 6, Sal k 7, Ses i 1, Ses i 2,
Ses i 3, Ses i 4, Ses i 5, Ses i 6, Ses i 7, Ses i 8, Sin a 1, Sin a 2, Sin a 3, Sin a 4, Sol i 1, Sol i 2, Sol i
3, Sol i 4, Sola 1 1, Sola 1 2, Sola 1 3, Sola 1 4, Sola 1 5, Sola 1 6, Sola 1 7, Sola t 1, Sola t 2, Sola t 3, Sola t 4, Sola t 8, Tri a 1, Tri a 2, Tri a 3, Tri a 4, Tri a 5, Tri a 7, Tri a 12, Tri a 13, Tri a 14, Tri a 15, Tri a 17, Tri a 18, Tri a 19, Tri a 19.0101, Tri a 20, Tri a 21, Tri a 25, Tri a 26, Tri a 27, Tri a 28, Tri a
29, Tri a 30, Tri a 31, Tri a 32, Tri a 33, Tri a 34, Tri a 35, Tri a 36, Tri a 37, Tri a 39, Tri a 40, Tri a 41,
Tri a 42, Tri a 43, Tri a 44, Tri a 45, Tri a Gliadin,
Tri a aA_TI, Tri a 191_369, Tri a 36, m43, m82, 10/Serine, 37/Thiore, 38/GTT, 112/1-Cys, 126/Dehy, Purothionin, Ves v 1, Ves v 2, Ves v 3, Ves v 5, Ves v 6, Zea m 1, Zea m 2, Zea m 3, Zea m 4, Zea m 5, Zea m 6, Zea m 7, Zea m 8, Zea m 11, Zea m 12, Zea m 13, Zea m 14, Zea m 22, Zea m 25, Zea m 32, Beta amylase, Avenin and GG1, preferably from the group consisting of Alpha Gal, Act d 1, Act d 2, Act d 5, Act d 8, Ain g 1, Alt a 1, Alt a 6,
Amb a 1 , Amb a 4 , Amb a 5 , Amb a 6 , Amb a 9 , Amb a 10,
Ana o 1, Ana o 2, Ana o 3, Ani s 1, Ani s 3, Api g 1, Api m 1, Api m 2, Api m 4, Ara h 1, Ara h 2, Ara h 3, Ara h 6, Ara h 8, Ara h 9, Art v 1, Art v 3, Asp f 1, Asp f 3,
Asp f 6, Ber e 1, Bet v 1, Bet v 2, Bet v 4, Bia g 1, Bia g 2, Bia g 5, Bia g 7, Bio t 5, Bos d 4, Bos d 5, Bos d Lf, Bos d 8, aSl, aS2, b-casein, k-casein, Transferrin, BSA, Bos d 6, Can f 1, Can f 2, Can f 3, Can f 4, Can f
5, Can f 6, Che a 1, Cla h 8, Cor a 1.0401, Cor a 8, Cor a 9, Cor a 14, Cry j 1, Cyn d 1, Cup a 1, Der p 1, Der f
1, Der p 2, Der f 2, Der p 4, Der p 5, Der p 7, Der p 10,
Der p 11, Der p 14, Der p 15, Der p 18, Der p 21, Der p
23, Der p 37, Lep d 2, Equ c 1, Equ c 3, Fag e 2, Eel d
1, Eel d 2, Eel d 4, Gad c 1, Gal d 1, Gal d 2, Gal d 3,
Gal d 5, Gly m 4, Gly m 5, Gly m 6, Hev b 1, Hev b 3, Hev b 5, Hev b 6.01, Jug r 1, Jug r 2, Jug r 3, Mai d 1, Mer a 1, Mus m 1, MUXF3, Ole e 1, Ole e 5, Ole e 6, Ole e 7,
Ole e 8, Ole e 9, Ole e 10, Par j 2, Pen m 1, Pen m 2,
Pen m 4, Phi p 1, Phi p 2, Phi p 4, Phi p 5b, Phi p 6,
Phi p 7, Phi p 11, Phi p 12, Pis v 3, Pla a 1, Pla a 2, Pla a 3, Pla 1 1, Pol d 5, Pru du 3, Pru du 4, Pru du 6, Pru du 6.01, Pru du 6.02, Pru p 1, Pru p 3, Sal k 1, Ses i 1, Tri a 14, Tri a 19.0101, Tri a aA_TI, Tri a 191_369, Tri a 36, m43, m82, 10/Serine, 37/Thiore, 38/GTT, 112/1- Cys, 126/Dehy, Purothionin, Ves v 1, Ves v 5, Beta amylase, Avenin and GG1.
[0030] The solid support elements of the present invention can be assembled or mounted on devices which can be used for analytical methods thereafter. In a preferred embodiment of the present invention the solid support elements are used to determine the presence or quantity of antibodies, in particular of IgA, IgE, IgG, IgGl, IgG2, IgG3, IgG4 and IgM immunoglobulins, binding to a an allergen or fragment thereof present in a sample (e.g. blood, plasma, serum, sputum) . The determination of the presence of these antibodies is useful in allergy diagnosis to determine whether an individual suffers from an allergy.
[0031] Another aspect of the present invention relates to a method for producing a device comprising at least one protein and/or peptide array comprising the steps of
- providing a solid support according to the present invention,
- breaking a circular predetermined breaking point of the solid support to remove a solid support element comprising a protein and/or nucleic acid peptide array and
- mounting said solid support element on a chip, microarray ELISA plate or ELISA plate-like carrier .
[0032] One or more solid support elements can be broken or cut out of the solid support of the present invention comprising one or more, preferably more than two, protein and/or peptide arrays by applying force or using means for cutting (e.g. blade) . Thereafter, these solid support elements are mounted reversible or irreversible on devices which are used in analytical methods (e.g. chips, microarray ELISA plates or ELISA plate-like carriers) . Reversible mounting of the solid support elements is particularly advantageous because it allows to reuse the device on which the solid support elements have been mounted .
[0033] The solid support elements are mounted to the aforementioned device by using a glue, magnetic means or any other mechanical means to fix the solid support elements on the device. Such fixing means are well known in the art .
[0034] The present invention is further illustrated in the following examples, without being restricted thereto.
EXAMPLES [0035] Exampl e 1 : Preparation of slide elements, slide element coating and allergen spotting [0036] Silicon chips of 8x17 mm with 90 nm silicon dioxide (SiCh) can be purchased from Silicon Valley Microelectronics, Inc. (USA) and mounted in frames (Ing. Pragler Ges. m.b.H., Austria) . Silicon chips and glass slides (Paul Marienfeld GmbH & Co. KG, Germany) are coated with MCP-2 (Lucidant Polymers, USA) diluted 1:100 in 0.9 M (NH4)2SO4 water solution. After incubation of slides with MCP-2 in the dark for 15 min at room temperature, the slides were rinsed with distilled H2O and dried using a bench centrifuge (200 g, 2 min) . Coated slides are stored under vacuum at 4 °C. Allergens are for example spotted in triplicate on MCP-2-coated silicon and glass slides using a SciFlex array spotter (Scienion, Germany) . Three hundred pL of each allergen (c=l mg/ml) are spotted at a distance of 500 pm from each other. The pH of allergen samples is adjusted with 750 mM Na2HPO4 buffer to pH 8.4. After spotting, the slides were incubated overnight in the dark in a 75 % humidity chamber to immobilize the allergens. After overnight incubation, the chips were sprayed with blocking buffer (30 mmol/L ethanolamine in PBS containing 0.1 % Tween-20) and incubated for 30 min at room temperature. After 30 min incubation, the slides were immersed in liquid plate sealer (CANDOR Bioscience GmbH, Germany) for 15 min. Slides are dried by centrifugation (200 g, 2 min) and stored under vacuum at 4 °C until use.
[0037] Exampl e 2 : Assays
[0038] Slides containing spotted allergens are immersed with wash buffer (PBS containing 1% Tween-20) and dried by centrifugation (200 g, 1 min) before serum application. Thirty microliter of undiluted (IgE detection) and diluted samples (serum and monoclonal IgE antibody) were incubated on microarray chips for 2 hours. For control purposes slides were incubated with sample diluent alone (Thermo Fisher Scientif ic/Phadia, Sweden) . After incubation, the slides are washed with wash buffer and dried by centrigfugation (200 g, 1 min) . Thirty microliter of a mouse monoclonal anti-human IgE antibody (Roche, Basel, Switzerland) and goat anti-human IgG antibody (Fab") 2 (Jackson ImmunoResearch, USA) (c= 1 mg/ml) conjugated with DyLight™ 550-2xPEG NHS Ester (Thermo Fisher Scientific, USA) were added and incubated in the dark for 30 min at room temperature. The conjugation of the detection antibodies to DyLight™ 550- 2xPEG NHS Ester was performed according to the manufacturer' s instructions. Unbound antibodies were washed away with wash buffer and then with distilled H2O. Slides were dried by centrifugation (200 g, 2 min) . Fluorescence signals were detected by a TECAN Power Scanner (Austria) at 25% laser power and 50% photomultiplier (PMT) gain for IgE measurement and at 10% laser power and 10% PMT gain for IgG measurement. Fluorescence intensities are for example analyzed using Luxscan software (China) . The buffer control (i.e., result obtained only with buffer and detection antibodies) was subtracted from each of the result and median fluorescence intensity (FI) from triplicate data were analyzed. The cut-off values for IgE and IgG detection corresponding to 0.1 lU/mL were 100 and 10 fluorescence intensities (FIs) for IgE and IgG, respectively .

Claims

CLAIMS :
1. A solid support comprising a set of protein and/or peptide arrays for determining allergen-specific antibodies, wherein said arrays are surrounded by a circular predetermined breaking point defining a solid support element.
2. Solid support according to claim 1, wherein the protein arrays comprise at least two immobilized allergens or fragments thereof.
3. Solid support according to claim 1 or 2, wherein the allergens are selected from the group consisting of the at least two allergens are selected from the group consisting of Alpha Gal, Act d 1, Act d 2, Act d 3, Act d 4, Act d 5, Act d 6, Act d 7, Act d 8, Act d 9, Act d 10, Act d 11, Act d 12, Act d 13, Aed a 1, Aed a 2, Aed a 3, Aed a 4, Aed a 5, Aed a
6, Aed a 7, Aed a 8, Aed a 10, Aed a 11, Ain g 1, Ain g 2, Ain g 4, Alt a 1, Alt a 2, Alt a 3, Alt a 4, Alt a 5, Alt a 6, Alt a 7, Alt a 8, Alt a 9, Alt a 10, Alt a 12, Alt a 13, Alt a 14, Al t a 15, Amb a 1 , Amb a 1.0101, Amb a 1.0201, Amb a 1.0202, Amb a 1.0301, Amb a 1.0302, Amb a 1.0303, Amb a 1.0304, Amb a 1.0305, Amb a 1.0401, Amb a 1.0402, Amb a 1.0501, Amb a 1.0502, Amb a 3 , Amb a 4 , Amb a 5 , Amb a 6 , Amb a 7 , Amb a 8 , Amb a 9, Amb a 10, Amb a 11, Amb a 12, Ana o 1, Ana o 2, Ana o
3, Ani s 1, Ani s 2, Ani s 3, Ani s 4, Ani s 5, Ani s 6, Ani s
7, Ani s 8, Ani s 9, Ani s 10, Ani s 11, Ani s 12, Ani s 13,
Ani s 14, Api g 1, Api g 2, Api g 3, Api g 4, Api g 5, Api g
6, Api m 1, Api m 2, Api m 3, Api m 4, Api m 5, Api m 6, Api m
7, Api m 8, Api m 9, Api m 10, Api m 11, Api m 12, Api m 13kD,
Ara h 1, Ara h 2, Ara h 3, Ara h 5, Ara h 6, Ara h 7, Ara h 8, Ara h 9, Ara h 10, Ara h 11, Ara h 12, Ara h 13, Ara h 14, Ara h 15, Ara h 16, Ara h 17, Art v 1, Art v 2, Art v 3, Art v 4, Art v 5, Art v 6, Asp f 1, Asp f 2, Asp f 3, Asp f 4, Asp f 5,
Asp f 6, Asp f 7, Asp f 8, Asp f 9, Asp f 10, Asp f 11, Asp f
12, Asp f 13, Asp f 14, Asp f 15, Asp f 16, Asp f 17, Asp f
18, Asp f 22, Asp f 23, Asp f 26, Asp f 27, Asp f 28, Asp f
29, Asp f 34, Ber e 1, Bet v 1, Bet v 2, Bet v 3, Bet v 4, Bet v 6, Bet v 7, Bet v 8, Bia g 1, Bia g 2, Bia g 3, Bia g 4, Bia g 5, Bia g 6, Bia g 7, Bia g 8, Bia g 9, Bia g 10, Bia g 11,
Bio t 1, Bio t 2, Bio t 3, Bio t 4, Bio t 5, Bio t 6, Bio t 7,
Bio t 8, Bio t 9, Bio t 10, Bio t 11, Bio t 12, Bio t 13, Bio t 14, Bio t 15, Bio t 18, Bio t 19, Bio t 20, Bio t 21, Bos d
1, Bos d 2, Bos d 3, Bos d 4, Bos d 5, Bos d 6, Bos d 7, Bos d
Lf, Bos d 8, Bos d 9, Bos d 11, Bos d 12, aSl, aS2, b-casein, k-casein, Transferrin, BSA, Bos d 6, Bra n 1, Bra n 4, Bra n
7, Bra n 8, Can f 1, Can f 2, Can f 3, Can f 4, Can f 5, Can f
6, Can f 7, Can f 8, Cas s 1, Cas s 2, Cas s 5, Cas s 8, Cas s
9, Cha o 1, Cha o 2, Cha o 3, Che a 1, Cla h 1, Cla h 2, Cla h
5, Cla h 6, Cla h 7, Cla h 8, Cla h 9, Cla h 10, Cla h 12, Cla h 13, Cor a 1, Cor a 1.0401, Cor a 2, Cor a 6, Cor a 8, Cor a
9, Cor a 10, Cor a 11, Cor a 12, Cor a 13, Cor a 14, Cry j 1,
Cry j 2, Cry j 3, Cry j 4, Cyn d 1, Cup a 1, Cup a 2, Cup a 3,
Cup a 4, Cup s 1, Cup s 2, Cup s 3, Cyn d 1, Cyn d 2, Cyn d 4,
Cyn d 5, Cyn d 6, Cyn d 7, Cyn d 11, Cyn d 12, Cyn d 13, Cyn d
15, Cyn d 22, Cyn d 23, Cyn d 24, Cyp c 1, Dau c 1, Dau c 3, Dau c 4, Dau c 5, Der p 1, Der f 1, Der p 2, Der f 2, Der p 3,
Der p 4, Der p 5, Der p 6, Der p 7, Der p 8, Der p 9, Der p
10, Der p 11, Der p 13, Der p 14, Der p 15, Der p 18, Der p
20, Der p 21, Der p 23, Clone 16, Der p 24, Der p 36, Der p
37, Lep d 2, Equ c 1, Equ c 2, Equ c 3, Equ c 4, Equ c 6, Equ c 8, Equ c 9, Equ c 10, Equ c 11, Fag e 1, Fag e 2, Fag e 3, Fag e 4, Fag e 5, Fag s 1, Fag s 2, Fag s 4, Eel d 1, Eel d 2,
Eel d 3, Eel d 4, Eel d 5, Eel d 6, Eel d 7, Eel d 8, Fra a 1,
Fra a 3, Fra a 4, Fra e 1, Fra e 2, Fra e 3, Fra e 6, Fra e 7,
Fra e 9, Fra e 10, Fra e 11, Fra e 12, Gad c 1, Gad m 1, Gad m
2, Gad m 3, Gad m 4, Gal d 1, Gal d 2, Gal d 3, Gal d 4, Gal d
5, Gal d 6, Gal d 7, Gal d 8, Gal d 9, Gal d 10, Gly m 1, Gly m 2, Gly m 3, Gly m 4, Gly m 5, Gly m 6, Gly m 7, Gly m 8, Hel a 1, Hel a 2, Hel a 3, Hel a 4, Hel a 6, Hev b 1, Hev b 2, Hev b 3, Hev b 4, Hev b 5, Hev b 6, Hev b 6.01, Hev b 7, Hev b 8,
Hev b 9, Hev b 10, Hev b 11, Hev b 12, Hev b 13, Hev b 14, Hev b 15, Hom a 1, Hom a 3, Hom a 4, Hom a 6, Hum j 1, Hum j 2,
Hum j 3, Jug n 1, Jug n 2, Jug n 4, Jug r 1, Jug r 2, Jug r 3,
Jug r 4, Jug r 5, Jug r 6, Jug r 7, Jug r 8, Jun a 1, Jun a 2,
Jun a 3, Len c 1, Len c 2, Len c 3, Lep d 2, Lep d 3, Lep d 5, 15
Lep d 7, Lep d 8, Lep d 10, Lep d 12, Lep d 13, Lep d 33, Mai d 1, Mai d 2, Mai d 3, Mai d 4, Mala s 1, Mala s 4, Mala s 5, Mala s 6, Mala s 7, Mala s 8, Mala s 9, Mala s 10, Mala s 11, Mala s 12, Mala s 13, Mer a 1, Mus m 1, Mus m 2, Mus m 4, Mus m 7, MUXF3, Ole e 1, Ole e 2, Ole e 3, Ole e 4, Ole e 5, Ole e
6, Ole e 7, Ole e 8, Ole e 9, Ole e 10, Ole e 11, Ole e 12,
Ole e 13, Ole e 14, Ole e 15, Ory c 1, Ory c 3, Ory c 4, Ory c
6, Ory c 8, Ory s 1, Ory s 2, Ory s 3, Ory s 7, Ory s 11, Ory s 12, Ory s 13, Ory s 14, Par j 1, Par j 2, Par j 3, Par j 4, Pen c 1, Pen c 2, Pen c 3, Pen c 6, Pen c 13, Pen c 18, Pen c
19, Pen c 22, Pen c 24, Pen c 30, Pen c 32, Pen m 1, Pen m 2,
Pen m 3, Pen m 4, Pen m 6, Pen m 8, Per a 1, Per a 2, Per a 3,
Per a 4, Per a 5, Per a 6, Per a 7, Per a 8, Per a 9, Per a
10, Per a 11, Per a 12, Phi p 1, Phi p 2, Phi p 3, Phi p 4,
Phi p 5, Phi p 5b, Phi p 6, Phi p 7, Phi p 11, Phi p 12, Phi p 13, Pis s 1, Pis s 2, Pis s 3, Pis s 5, Pis s 6, Pis v 3, Pla a 1, Pla a 2, Pla a 3, Pla a 8, Pla 1 1, Pol d 5, Pru av 1, Pru av 2, Pru av 3, Pru av 4, Pru du 1, Pru du 2, Pru du 3,
Pru du 4, Pru du 5, Pru du 6, Pru du 6.01, Pru du 6.02, Pru p
1, Pru p 2, Pru p 3, Pru p 4, Pru p 7, Que a 1, Que a 2, Que a
4, Rat n 1, Rat n 4, Rat n 7, Rat n 8, Sal k 1, Sal k 2, Sal k
3, Sal k 4, Sal k 5, Sal k 6, Sal k 7, Ses i 1, Ses i 2, Ses i
3, Ses i 4, Ses i 5, Ses i 6, Ses i 7, Ses i 8, Sin a 1, Sin a
2, Sin a 3, Sin a 4, Sol i 1, Sol i 2, Sol i 3, Sol i 4, Sola
1 1, Sola 1 2, Sola 1 3, Sola 1 4, Sola 1 5, Sola 1 6, Sola 1
7, Sola t 1, Sola t 2, Sola t 3, Sola t 4, Sola t 8, Tri a 1,
Tri a 2, Tri a 3, Tri a 4, Tri a 5, Tri a 7, Tri a 12, Tri a 13, Tri a 14, Tri a 15, Tri a 17, Tri a 18, Tri a 19, Tri a 19.0101, Tri a 20, Tri a 21, Tri a 25, Tri a 26, Tri a 27, Tri a 28, Tri a 29, Tri a 30, Tri a 31, Tri a 32, Tri a 33, Tri a
34, Tri a 35, Tri a 36, Tri a 37, Tri a 39, Tri a 40, Tri a
41, Tri a 42, Tri a 43, Tri a 44, Tri a 45, Tri a Gliadin, Tri a aA_TI, Tri a 191_369, Tri a 36, m43, m82, 10/Serine, 37/Thiore, 38/GTT, 112/1-Cys, 126/Dehy, Purothionin, Ves v 1, Ves v 2, Ves v 3, Ves v 5, Ves v 6, Zea m 1, Zea m 2, Zea m 3, Zea m 4, Zea m 5, Zea m 6, Zea m 7, Zea m 8, Zea m 11, Zea m 12, Zea m 13, Zea m 14, Zea m 22, Zea m 25, Zea m 32, Beta amylase, Avenin and GG1, preferably from the group consisting 16 of Alpha Gal, Act d 1, Act d 2, Act d 5, Act d 8, Ain g 1, Alt a 1 , Al t a 6 , Amb a 1 , Amb a 4 , Amb a 5 , Amb a 6 , Arnb a 9 , Amb a 10, Ana o 1, Ana o 2, Ana o 3, Ani s 1, Ani s 3, Api g 1,
Api m 1, Api m 2, Api m 4, Ara h 1, Ara h 2, Ara h 3, Ara h 6,
Ara h 8, Ara h 9, Art v 1, Art v 3, Asp f 1, Asp f 3, Asp f 6,
Ber e 1, Bet v 1, Bet v 2, Bet v 4, Bia g 1, Bia g 2, Bia g 5,
Bia g 7, Bio t 5, Bos d 4, Bos d 5, Bos d Lf, Bos d 8, aSl, aS2, b-casein, k-casein, Transferrin, BSA, Bos d 6, Can f 1, Can f 2, Can f 3, Can f 4, Can f 5, Can f 6, Che a 1, Cla h 8,
Cor a 1.0401, Cor a 8, Cor a 9, Cor a 14, Cry j 1, Cyn d 1,
Cup a 1, Der p 1, Der f 1, Der p 2, Der f 2, Der p 4, Der p 5,
Der p 7, Der p 10, Der p 11, Der p 14, Der p 15, Der p 18, Der p 21, Der p 23, Der p 37, Lep d 2, Equ c 1, Equ c 3, Fag e 2, Eel d 1, Eel d 2, Eel d 4, Gad c 1, Gal d 1, Gal d 2, Gal d 3,
Gal d 5, Gly m 4, Gly m 5, Gly m 6, Hev b 1, Hev b 3, Hev b 5,
Hev b 6.01, Jug r 1, Jug r 2, Jug r 3, Mai d 1, Mer a 1, Mus m
1, MUXF3, Ole e 1, Ole e 5, Ole e 6, Ole e 7, Ole e 8, Ole e 9, Ole e 10, Par j 2, Pen m 1, Pen m 2, Pen m 4, Phi p 1, Phi p 2, Phi p 4, Phi p 5b, Phi p 6, Phi p 7, Phi p 11, Phi p 12, Pis v 3, Pla a 1, Pla a 2, Pla a 3, Pla 1 1, Pol d 5, Pru du
3, Pru du 4, Pru du 6, Pru du 6.01, Pru du 6.02, Pru p 1, Pru p 3, Sal k 1, Ses i 1, Tri a 14, Tri a 19.0101, Tri a aA_TI, Tri a 191_369, Tri a 36, m43, m82, 10/Serine, 37/Thiore, 38/GTT, 112/1-Cys, 126/Dehy, Purothionin, Ves v 1, Ves v 5, Beta amylase, Avenin and GG1.
4. Solid support according to any one of claims 1 to 3, wherein the solid support comprises or consists of silicon, a plastic material or a metal.
5. Solid support according to any one of claims 1 to 4, wherein the solid support is a silicon wafer.
6. Solid support according to any one of claims 1 to 6, wherein the circular predetermined breaking point is an encircling weakness of the cross section, a groove, encircling perforations or a combination thereof. 17
7 . Method for producing a device comprising a protein and/or peptide array comprising the steps of
- providing a solid support according to any one of claims 1 to 6 ,
- breaking a circular predetermined breaking point of the solid support to remove a solid support element comprising a protein and/or peptide array and
- mounting said sol id support element on a chip, microarray ELISA plate or ELISA plate-like carrier .
PCT/AT2021/060428 2020-11-11 2021-11-11 Solid support comprising a set of protein arrays WO2022099343A1 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3045199A1 (en) * 1980-12-01 1982-07-01 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Solid-phase immunoassay method - using reagent plate with perforated wells fitting into wells in sample plate
DE19609315A1 (en) * 1996-03-09 1997-09-18 Univ Dresden Tech Nasal applicator
DE29923907U1 (en) * 1999-12-01 2001-07-26 Quantifoil Micro Tools Gmbh Miniaturized slide for carrying out a large number of test series in the submicroliter range
EP1415788A1 (en) * 2002-10-31 2004-05-06 Agilent Technologies, Inc. Integrated microfluidic array device

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4322180A1 (en) * 1993-07-03 1995-01-12 Merck Patent Gmbh Perforated films for thin-layer chromatography
DE10242066B4 (en) * 2002-09-11 2005-03-17 Cytopharm Gmbh Process for the treatment of cell cultures

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3045199A1 (en) * 1980-12-01 1982-07-01 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Solid-phase immunoassay method - using reagent plate with perforated wells fitting into wells in sample plate
DE19609315A1 (en) * 1996-03-09 1997-09-18 Univ Dresden Tech Nasal applicator
DE29923907U1 (en) * 1999-12-01 2001-07-26 Quantifoil Micro Tools Gmbh Miniaturized slide for carrying out a large number of test series in the submicroliter range
EP1415788A1 (en) * 2002-10-31 2004-05-06 Agilent Technologies, Inc. Integrated microfluidic array device

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "SeraSpot", 1 February 2020 (2020-02-01), pages 1 - 4, XP055876976, Retrieved from the Internet <URL:https://clinical.r-biopharm.com/wp-content/uploads/2020/04/seraspot_4p_en_2020-02.pdf> [retrieved on 20220110] *
DI FRAIA MARCO ET AL: "A new molecular multiplex IgE assay for the diagnosis of pollen allergy in Mediterranean countries: A validation study", CLINICAL & EXPERIMENTAL ALLERGY, vol. 49, no. 3, 1 March 2019 (2019-03-01), UK, pages 341 - 349, XP055877245, ISSN: 0954-7894, Retrieved from the Internet <URL:https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fcea.13264> DOI: 10.1111/cea.13264 *
JAKOB T ET AL., ALLERGO J, vol. 24, no. 8, 2015, pages 42 - 56
JAYARAMAN VASANTH ET AL: "An ultra-high-density protein microarray for high throughput single-tier serological detection of Lyme disease", SCIENTIFIC REPORTS, vol. 10, no. 1, 22 October 2020 (2020-10-22), XP055877061, Retrieved from the Internet <URL:https://www.nature.com/articles/s41598-020-75036-2.pdf> DOI: 10.1038/s41598-020-75036-2 *
LUPINEK C ET AL., METHODS, vol. 66, no. 1, 2014, pages 106 - 119

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