WO2022096917A1 - Dispositif et méthode de fragmentation de graisse - Google Patents

Dispositif et méthode de fragmentation de graisse Download PDF

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Publication number
WO2022096917A1
WO2022096917A1 PCT/IB2020/060324 IB2020060324W WO2022096917A1 WO 2022096917 A1 WO2022096917 A1 WO 2022096917A1 IB 2020060324 W IB2020060324 W IB 2020060324W WO 2022096917 A1 WO2022096917 A1 WO 2022096917A1
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WO
WIPO (PCT)
Prior art keywords
filter
adipose tissue
size
optionally
combination
Prior art date
Application number
PCT/IB2020/060324
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English (en)
Inventor
Marcos SFORZA
Original Assignee
The Duke Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Duke Limited filed Critical The Duke Limited
Priority to CN202080052202.4A priority Critical patent/CN114729296A/zh
Priority to PCT/IB2020/060324 priority patent/WO2022096917A1/fr
Priority to KR1020217043371A priority patent/KR20230103754A/ko
Priority to EP20938505.3A priority patent/EP4021466A4/fr
Publication of WO2022096917A1 publication Critical patent/WO2022096917A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/35—Fat tissue; Adipocytes; Stromal cells; Connective tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/05—Means for pre-treatment of biological substances by centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Definitions

  • the present invention relates to a fat harvesting and processing device and method.
  • the present invention relates to a device and method for adipose tissue harvesting, microfragmentation, facilitation of stem cell extraction and stem cell mechanical separation and nanofragmentation.
  • Adipose tissue is a source of stem cells for various tissue engineering and cell therapies.
  • the stromal vascular fraction (“SVF”) derived from adipose tissue is harvested and processed, and in medical and cosmetic procedures, such SVF is used alone or with another material to generate a graft material for application to a subject.
  • SVF contains adipose-derived stem cells (ADSCs).
  • ADSCs adipose-derived stem cells
  • ADSCs Various techniques are developed, with limited success, aiming to minimize injury to ADSCs and exposure of ADSCs to various risk factors including environmental stress such as mechanical impact, temperature and pressure shocks, and chemical and biochemical exposures (exposure to viral or bacterial pathogens), which often lead to injury or death of ADSCs, which in turn, would illicit various adverse biochemical reactions (e.g., secretion of adverse cytokines or adverse immune reactions), leading to ultimate failure of such biomedical and cosmetic applications or procedures.
  • environmental stress such as mechanical impact, temperature and pressure shocks, and chemical and biochemical exposures (exposure to viral or bacterial pathogens)
  • adverse biochemical reactions e.g., secretion of adverse cytokines or adverse immune reactions
  • a device for adipose tissue processing, microfragmentation and facilitation of mechanical separation of adipose derived stem cells comprising: an upper housing with an inlet, a lower housing with an outlet, a filter stack, and a spiral flow effectuate, wherein the upper housing and the lower housing are configured to join to form an enclosure that encloses the filter stack and the spiral flow effectuater, wherein the spiral effectuate is configured to receive a flower of filtrate from the filter stack and generate a spiral flow of the adipose tissue to minimize a direct shock of the adipose tissue with walls of the filter stacker to promote enhanced mechanical separation with less trauma to cells of the adipose tissue.
  • ADSCs adipose derived stem cells
  • the filter stack comprises at least one filter having multiple holes of a size that is the same or different, the size ranging from about 0.4 mm to about 3 mm.
  • the filter stack comprises at least one filter having multiple holes of alternating sizes.
  • the filter stack comprises a first filter, a second filter, and a third filter, where - the first filter having multiple holes of a size that is the same ranging from about 2.2 mm to about 1.45 mm; the second filter having multiple holes of alternating sizes where one size that is the same ranging from about 1.8 mm to 0.9 mm and another size that is the same ranging from about 1.35 mm to about 0.6 mm; and the third filter having multi holes of a size that is the same ranging from about 1.35 mm to about 0.45 mm.
  • the upper housing and lower housing are joined by a tongue and groove joint joined by ultrasonic welding.
  • the filter stack comprises more than one filters, and the more than one filters are connected via rods or tubes.
  • the inlet and outlet comprise a Luer lock thread.
  • the at least one of the upper housing, lower housing, the filter stack or the spiral flow effectuater is made from polycarbonate or stainless steel.
  • the device is a disposable device.
  • a method of producing stromal vascular fraction of adipose tissue comprising subjecting a volume of adipose tissue to fragmentation and separation by a device to generate a volume of fragmented adipose tissue; and subject the fragmented adipose tissue to centrifuging to generate a volume of the stromal vascular fraction
  • the device is a device for adipose tissue processing, microfragmentation and facilitation of mechanical separation of adipose derived stem cells (“ADSCs”), the device comprising: an upper housing with an inlet, a lower housing with an outlet, a filter stack, and a spiral flow effectuate, where - the upper housing and the lower housing are configured to join to form an enclosure that encloses the filter stack and the spiral flow effectuater; and the spiral effectuate is configured to receive a flower of filtrate from the filter stack and generate a spiral flow of the adi
  • the filter stack comprises at least one filter having multiple holes of a size that is the same or different, the size ranging from about 0.4 mm to about 3 mm.
  • the filter stack comprises at least one filter having multiple holes of alternating sizes.
  • the filter stack comprises a first filter, a second filter, and a third filter, where - the first filter having multiple holes of a size that is the same ranging from about 2.2 mm to about 1.45 mm; the second filter having multiple holes of alternating sizes where one size that is the same ranging from about 1.8 mm to 0.9 mm and another size that is the same ranging from about 1.35 mm to about 0.6 mm; and the third filter having multi holes of a size that is the same ranging from about 1.35 mm to about 0.45 mm.
  • the upper housing and lower housing are joined by a tongue and groove joint joined by ultrasonic welding.
  • the filter stack comprises more than one filters, and the more than one filters are connected via rods or tubes.
  • the inlet and outlet comprise a Luer lock thread.
  • the at least one of the upper housing, lower housing, the filter stack or the spiral flow effectuater is made from polycarbonate or stainless steel.
  • the device is a disposable device.
  • a graft comprising a volume of the stromal vascular fraction (“SVF”) generated by a method of invention, the method comprising subjecting a volume of adipose tissue to fragmentation and separation by a device to generate a volume of fragmented adipose tissue; and subject the fragmented adipose tissue to centrifuging to generate a volume of the SVF, wherein the device is a device for adipose tissue processing, microfragmentation and facilitation of mechanical separation of adipose derived stem cells (“ADSCs”), the device comprising: an upper housing with an inlet, a lower housing with an outlet, a filter stack, and a spiral flow effectuate, where - the upper housing and the lower housing are configured to join to form an enclosure that encloses the filter stack and the spiral flow effectuater; and the spiral effectuate is configured to receive a flower of filtrate from the filter stack and
  • the device is a device for adipose tissue processing
  • the filter stack comprises at least one filter having multiple holes of a size that is the same or different, the size ranging from about 0.4 mm to about 3 mm.
  • the filter stack comprises at least one filter having multiple holes of alternating sizes.
  • the filter stack comprises a first filter, a second filter, and a third filter, where - the first filter having multiple holes of a size that is the same ranging from about 2.2 mm to about 1.45 mm; the second filter having multiple holes of alternating sizes where one size that is the same ranging from about 1.8 mm to 0.9 mm and another size that is the same ranging from about 1.35 mm to about 0.6 mm; and the third filter having multi holes of a size that is the same ranging from about 1.35 mm to about 0.45 mm.
  • the upper housing and lower housing are joined by a tongue and groove joint joined by ultrasonic welding.
  • the filter stack comprises more than one filters, and the more than one filters are connected via rods or tubes.
  • the inlet and outlet comprise a Luer lock thread.
  • the at least one of the upper housing, lower housing, the filter stack or the spiral flow effectuater is made from polycarbonate or stainless steel.
  • the device is a disposable device.
  • the graft further comprises a pharmaceutically acceptable carrier.
  • the graft further comprises a volume of adipose tissue.
  • a method of treating a condition in a subject comprising administering a site of the subject in need thereof a graft of invention, the graft comprising a volume of the stromal vascular fraction (“SVF”) generated by a method of invention, the method comprising subjecting a volume of adipose tissue to fragmentation and separation by a device to generate a volume of fragmented adipose tissue; and subject the fragmented adipose tissue to centrifuging to generate a volume of the SVF, wherein the device is a device for adipose tissue processing, microfragmentation and facilitation of mechanical separation of adipose derived stem cells (“ADSCs”), the device comprising: an upper housing with an inlet, a lower housing with an outlet, a filter stack, and a spiral flow effectuate, where - the upper housing and the lower housing are configured to join to form an enclosure that encloses the
  • the filter stack comprises at least one filter having multiple holes of a size that is the same or different, the size ranging from about 0.4 mm to about 3 mm.
  • the filter stack comprises at least one filter having multiple holes of alternating sizes.
  • the filter stack comprises a first filter, a second filter, and a third filter, where - the first filter having multiple holes of a size that is the same ranging from about 2.2 mm to about 1.45 mm; the second filter having multiple holes of alternating sizes where one size that is the same ranging from about 1.8 mm to 0.9 mm and another size that is the same ranging from about 1.35 mm to about 0.6 mm; and the third filter having multi holes of a size that is the same ranging from about
  • the upper housing and lower housing are joined by a tongue and groove joint joined by ultrasonic welding.
  • the filter stack comprises more than one filters, and the more than one filters are connected via rods or tubes.
  • the inlet and outlet comprise a Luer lock thread.
  • the at least one of the upper housing, lower housing, the filter stack or the spiral flow effectuater is made from polycarbonate or stainless steel.
  • the device is a disposable device.
  • the graft further comprises a pharmaceutically acceptable carrier.
  • the graft further comprises a volume of adipose tissue.
  • the subject is a human being.
  • the site is a skeletal site, such as a joint or intervertebral, or a soft tissue site, such as breast, cheek or buttock or a scar or wound.
  • FIG. 1 shows an embodiment of the invention device.
  • FIG. 1A shows the structural components of a device embodiment;
  • FIG. IB shows the outside overall appearance of a device embodiment;
  • FIG. C shows the dimension of a device embodiment;
  • FIG. ID shows a cross-sectional view of a device embodiment.
  • FIG. 2 shows various views of an upper housing of an embodiment of the invention device.
  • FIG. 3 shows various views of an upper housing of an embodiment of the invention device.
  • FIG. 4 shows a top view and side perspective views of filter 2 of an embodiment of invention device.
  • FIG. 5 shows a top view and side perspective views of filter 1 of a filter stack of an embodiment of invention device.
  • FIG. 6 shows a top view and side perspective views of filter 3 of a filter stack of an embodiment of invention device.
  • FIG. 7 shows various views of a spiral of an embodiment of invention device.
  • FIG. 7A side view
  • FIG. IB top view
  • FIG. 1C top-side view.
  • FIG.8 shows the photo pictures of test results of cells separated by an embodiment of invention device and cells by a commercially available device.
  • the term “enhanced mechanical separation” refers to an enhanced degree of separation of stromal vascular fraction from adipose tissue without the aid of chemical or biochemical agents such as an enzyme.
  • the use of enzyme for cell separation is a technique to separate cells from adipose tissue called for by a need to achieve such, which itself indicates that without the using an agent, it would be much harder to separate cells from adipose tissue.
  • the term “less trauma to cells” refers to a lesser degree of trauma to cells relative to the degree of trauma to cells caused by cell separation from adipose tissue using a technology different than the one disclosed in this application, e.g., separation with enzymatic digestion or strong mechanical agitation.
  • condition refers to a medical or cosmetic condition that can be addressed by ADSCs or SVF or a graft containing any of these.
  • a device for adipose tissue processing, microfragmentation and facilitation of mechanical separation of adipose derived stem cells comprising: an upper housing with an inlet, a lower housing with an outlet, a filter stack, and a spiral flow effectuate, wherein the upper housing and the lower housing are configured to join to form an enclosure that encloses the filter stack and the spiral flow effectuater, wherein the spiral effectuate is configured to receive a flower of filtrate from the filter stack and generate a spiral flow of the adipose tissue to minimize a direct shock of the adipose tissue with walls of the filter stacker to promote enhanced mechanical separation with less trauma to cells of the adipose tissue.
  • ADSCs adipose derived stem cells
  • the filter stack comprises at least one filter having multiple holes of a size that is the same or different, the size ranging from about 0.4 mm to about 3 mm.
  • the filter stack comprises at least one filter having multiple holes of alternating sizes.
  • the filter stack comprises a first filter, a second filter, and a third filter, where - the first filter having multiple holes of a size that is the same ranging from about 2.2 mm to about 1.45 mm; the second filter having multiple holes of alternating sizes where one size that is the same ranging from about 1.8 mm to 0.9 mm and another size that is the same ranging from about 1.35 mm to about 0.6 mm; and the third filter having multi holes of a size that is the same ranging from about 1.35 mm to about 0.45 mm.
  • the upper housing and lower housing are joined by a tongue and groove joint joined by ultrasonic welding.
  • the filter stack comprises more than one filters, and the more than one filters are connected via rods or tubes.
  • the inlet and outlet comprise a Luer lock thread.
  • the at least one of the upper housing, lower housing, the filter stack or the spiral flow effectuater is made from polycarbonate or stainless steel.
  • the device is a disposable device.
  • a method of producing stromal vascular fraction of adipose tissue comprising subjecting a volume of adipose tissue to fragmentation and separation by a device to generate a volume of fragmented adipose tissue; and subject the fragmented adipose tissue to centrifuging to generate a volume of the stromal vascular fraction
  • the device is a device for adipose tissue processing, microfragmentation and facilitation of mechanical separation of adipose derived stem cells (“ADSCs”), the device comprising: an upper housing with an inlet, a lower housing with an outlet, a filter stack, and a spiral flow effectuate, where - the upper housing and the lower housing are configured to join to form an enclosure that encloses the filter stack and the spiral flow effectuater; and the spiral effectuate is configured to receive a flower of filtrate from the filter stack and generate a spiral flow of the adi
  • the filter stack comprises at least one filter having multiple holes of a size that is the same or different, the size ranging from about 0.4 mm to about 3 mm.
  • the filter stack comprises at least one filter having multiple holes of alternating sizes.
  • the filter stack comprises a first filter, a second filter, and a third filter, where - the first filter having multiple holes of a size that is the same ranging from about 2.2 mm to about 1.45 mm; the second filter having multiple holes of alternating sizes where one size that is the same ranging from about 1.8 mm to 0.9 mm and another size that is the same ranging from about 1.35 mm to about 0.6 mm; and the third filter having multi holes of a size that is the same ranging from about
  • the upper housing and lower housing are joined by a tongue and groove joint joined by ultrasonic welding.
  • the filter stack comprises more than one filters, and the more than one filters are connected via rods or tubes.
  • the inlet and outlet comprise a Luer lock thread.
  • the at least one of the upper housing, lower housing, the filter stack or the spiral flow effectuater is made from polycarbonate or stainless steel.
  • the device is a disposable device.
  • a graft comprising a volume of the stromal vascular fraction (“SVF”) generated by a method of invention, the method comprising subjecting a volume of adipose tissue to fragmentation and separation by a device to generate a volume of fragmented adipose tissue; and subject the fragmented adipose tissue to centrifuging to generate a volume of the SVF, wherein the device is a device for adipose tissue processing, microfragmentation and facilitation of mechanical separation of adipose derived stem cells (“ADSCs”), the device comprising: an upper housing with an inlet, a lower housing with an outlet, a filter stack, and a spiral flow effectuate, where - the upper housing and the lower housing are configured to join to form an enclosure that encloses the filter stack and the spiral flow effectuater; and the spiral effectuate is configured to receive a flower of filtrate from the filter stack and
  • the device is a device for adipose tissue processing
  • the filter stack comprises at least one filter having multiple holes of a size that is the same or different, the size ranging from about 0.4 mm to about 3 mm.
  • the filter stack comprises at least one filter having multiple holes of alternating sizes.
  • the filter stack comprises a first filter, a second filter, and a third filter, where - the first filter having multiple holes of a size that is the same ranging from about 2.2 mm to about 1.45 mm; the second filter having multiple holes of alternating sizes where one size that is the same ranging from about 1.8 mm to 0.9 mm and another size that is the same ranging from about 1.35 mm to about 0.6 mm; and the third filter having multi holes of a size that is the same ranging from about
  • the upper housing and lower housing are joined by a tongue and groove joint joined by ultrasonic welding.
  • the filter stack comprises more than one filters, and the more than one filters are connected via rods or tubes.
  • the inlet and outlet comprise a Luer lock thread.
  • the at least one of the upper housing, lower housing, the filter stack or the spiral flow effectuater is made from polycarbonate or stainless steel.
  • the device is a disposable device.
  • the graft further comprises a pharmaceutically acceptable carrier.
  • the graft further comprises a volume of adipose tissue.
  • a method of treating a condition in a subject comprising administering a site of the subject in need thereof a graft of invention, the graft comprising a volume of the stromal vascular fraction (“SVF”) generated by a method of invention, the method comprising subjecting a volume of adipose tissue to fragmentation and separation by a device to generate a volume of fragmented adipose tissue; and subject the fragmented adipose tissue to centrifuging to generate a volume of the SVF, wherein the device is a device for adipose tissue processing, microfragmentation and facilitation of mechanical separation of adipose derived stem cells (“ADSCs”), the device comprising: an upper housing with an inlet, a lower housing with an outlet, a filter stack, and a spiral flow effectuate, where - the upper housing and the lower housing are configured to join to form an enclosure that encloses the
  • the filter stack comprises at least one filter having multiple holes of a size that is the same or different, the size ranging from about
  • the filter stack comprises at least one filter having multiple holes of alternating sizes.
  • the filter stack comprises a first filter, a second filter, and a third filter, where - the first filter having multiple holes of a size that is the same ranging from about 2.2 mm to about 1.45 mm; the second filter having multiple holes of alternating sizes where one size that is the same ranging from about 1.8 mm to 0.9 mm and another size that is the same ranging from about 1.35 mm to about 0.6 mm; and the third filter having multi holes of a size that is the same ranging from about 1.35 mm to about 0.45 mm.
  • the upper housing and lower housing are joined by a tongue and groove joint joined by ultrasonic welding.
  • the filter stack comprises more than one filters, and the more than one filters are connected via rods or tubes.
  • the inlet and outlet comprise a Luer lock thread.
  • the at least one of the upper housing, lower housing, the filter stack or the spiral flow effectuater is made from polycarbonate or stainless steel.
  • the device is a disposable device.
  • the graft further comprises a pharmaceutically acceptable carrier.
  • the graft further comprises a volume of adipose tissue.
  • the subject is a human being.
  • the site is a skeletal site, such as a joint or intervertebral, or a soft tissue site, such as breast, cheek or buttock or a scar or wound.
  • Centrifuge fat from 2.5 to 3.5 min at 3000 rpm until one obtains about 20ml of centrifuged fat.
  • the SVF can be used to be directly injected on scars, wounds or joints.
  • the SVF can be mixed with fat for autologous injection.
  • Example 1 Construction of a device and method for adipose tissue harvesting, microfragmentation, facilitation of stem cell extraction and stem cell mechanical separation and nanofragmentation (“SPING”)
  • FIGs. 1-7 show the SPING device made from polycarbonate.
  • FIG. 1 A shows the structural components of the SPING device, having a filter stack having Filter 1, Filter 2, Filter 3, a spiral 4, an upper housing 5, and a lower housing 6;
  • FIG. IB shows the outside overall appearance of the SPING device;
  • FIG. C shows the dimension of the SPING device;
  • FIG. ID shows a cross-sectional view of the SPING device.
  • FIG. 2 shows various views of an upper housing 5 of the SPING device: FIG. 2A, a perspective, side view; FIG. 2B, cross-sectional view; FIG. 2C, bottom view, which faces and joins a lower housing 6 (FIG. 3); and FIG. 2D, overall appearance.
  • 501 in FIGs. 2A, B and D is a Luer Lock thread.
  • FIG. 3 shows various views of an upper housing of the SPING device: FIG. 3A, a perspective, side view; FIG. 3B, cross-sectional view; FIG. 3C, top view, which faces and joins an upper housing 5 (FIG. 2); and FIG. 3D, overall appearance, showing the internal housing space of the lower housing 5.
  • 601 in FIGs. 3 A, B and D is a Luer Lock thread.
  • FIG. 4 shows a top view and side perspective views of Filter 2 of the SPING device: FIG. 4A, top view, showing pores of a diameter in mm 201 ; FIG. 4B, side view, showing pore channels 202.
  • FIG. 5 shows a top view and side perspective views of Filter 1 of a filter stack of the SPING device: FIG. 5 A, top view, showing pores 101 of two different diameters in mm (larger diameter) and 101’ (smaller diameter); FIG. 5B, side view, showing pore channels 102.
  • FIG. 6 shows a top view and side perspective views of Filter 3 of a filter stack of the SPING device: FIG. 6A, top view, showing pores 301 of a diameter in mm; FIG. 6A, top view, showing pores 301 of a diameter in mm; FIG. 6A, top view, showing pores 301 of a diameter in mm; FIG. 6A, top view, showing pores 301 of a diameter in mm; FIG. 6A, top view, showing pores 301 of a diameter in mm; FIG.
  • FIG. 7 shows various views of a spiral of an embodiment of invention device: FIG. 7A, side view; FIG. 7B, top view; FIG. 7C, top-side view; where 401 is the spiral component, 402 is opening for filtrate fluid.
  • SPING devices constructed in Example 1 were used for cell separation and cell viability studies. Cell separation and cell viability studies were also performed on a device by TONNARD Technique (“Tonnard Technique device”). Adipose tissue was harvested from 5 female at the abdomen, which was stored at 4 °C for 24 hrs before use. SPRING prototype plastic: mechanical dissociation 20 passes (MS 20);
  • Tonnard Technique device Luer to Luer, 30 passes.
  • Table 1 shows the SPING device (metal: stainless steel) provides the best cell separation result and SVF yield. Compared to Tonnard devices, SPING devices produced SVF cells almost twice as many, which is significant.
  • Example 2 The results shown in Example 2 are even more convincing and demonstrate that SPING devices generate SVF cells almost twice as efficient as compared with the Tonnard devices.
  • the culture cell viability studies clearly demonstrate that due to reduced impact on cells by SPING device and that the SVF are convincingly far more viable as compared with SVF cells obtained by Tonnard devices, which is very significant in cell regeneration and cell therapy.

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Abstract

La présente invention concerne un dispositif pour le traitement de tissus adipeux, leur microfragmentation et la facilitation de la séparation mécanique de cellules souches dérivées de tissus adipeux ("ADSC"), des méthodes d'utilisation du dispositif pour générer une fraction vasculaire stromale ("FVS") et des méthodes associées à la FVS.
PCT/IB2020/060324 2020-11-03 2020-11-03 Dispositif et méthode de fragmentation de graisse WO2022096917A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN202080052202.4A CN114729296A (zh) 2020-11-03 2020-11-03 一种脂肪碎解器械及方法
PCT/IB2020/060324 WO2022096917A1 (fr) 2020-11-03 2020-11-03 Dispositif et méthode de fragmentation de graisse
KR1020217043371A KR20230103754A (ko) 2020-11-03 2020-11-03 지방 파편화 장치 및 방법
EP20938505.3A EP4021466A4 (fr) 2020-11-03 2020-11-03 Dispositif et méthode de fragmentation de graisse

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PCT/IB2020/060324 WO2022096917A1 (fr) 2020-11-03 2020-11-03 Dispositif et méthode de fragmentation de graisse

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WO2022096917A1 true WO2022096917A1 (fr) 2022-05-12

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EP (1) EP4021466A4 (fr)
KR (1) KR20230103754A (fr)
CN (1) CN114729296A (fr)
WO (1) WO2022096917A1 (fr)

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WO2019145276A1 (fr) 2018-01-25 2019-08-01 Bh Swiss Sa Dispositif de production de globules de tissu adipeux
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WO2020008805A1 (fr) * 2018-07-05 2020-01-09 富士フイルム株式会社 Dispositif de séparation cellulaire
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US20190232017A1 (en) * 2010-05-20 2019-08-01 Lipogems International S.P.A. Method for preparing tissue, particularly adipose tissue, for transplantation from lobular fat extracted by liposuction
US20130344589A1 (en) * 2012-06-22 2013-12-26 Human Med Ag Device for separating adult stem cell
US20150374888A1 (en) 2014-05-15 2015-12-31 Ronald D. Shippert Tissue parcelization method and apparatus
US20200131478A1 (en) 2015-02-19 2020-04-30 Lifecell Corporation Tissue processing device and associated systems and methods
WO2019012231A1 (fr) * 2017-07-12 2019-01-17 Stemcis Dispositif de fragmentation mécanique de tissus destine a la preparation d'une composition de cellules isolees, procede correspondant
WO2019145276A1 (fr) 2018-01-25 2019-08-01 Bh Swiss Sa Dispositif de production de globules de tissu adipeux
WO2020008805A1 (fr) * 2018-07-05 2020-01-09 富士フイルム株式会社 Dispositif de séparation cellulaire
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EP4021466A4 (fr) 2023-07-19
CN114729296A (zh) 2022-07-08
KR20230103754A (ko) 2023-07-07

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