WO2022089618A1 - Multifunctional sperm quality analyzer, sperm quality detection method, and computer-readable storage medium - Google Patents

Abstract

A multifunctional sperm quality analyzer, a sperm quality detection method, and a computer-readable storage medium. In the multifunctional sperm quality analyzer, a liquefaction processing mechanism (15) heats and liquefies a sample in a sample container by means of an incubation heating component (420); a sampling mechanism (11) aspirates the sample in the sample container by means of a sampling needle (230), and performs liquefaction detection; a detection processing mechanism is composed of a reaction processing mechanism (12) and/or a microscope inspection processing mechanism (13); and the reaction processing mechanism (12) is used for taking images of the sample before and after the injection of a reaction reagent in a reaction container (310) by means of first image capturing component (110); the microscope inspection processing mechanism (13) is used for focusing a sample on a detection card by means of a microscope, and capturing images of the sample on the detection card by means of a second image capturing component; and a controller (14) performs sequential control on the liquefaction processing mechanism (15), the sampling mechanism (11), the reaction processing mechanism (12) and/or the microscope inspection processing mechanism (13). The detection efficiency may not only be increased, but actual needs during the detection process may also be met.

Description

Multifunctional sperm quality analyzer, sperm quality detection method, and computer-readable storage medium

The present disclosure is based on the patent documents submitted on October 30, 2020 with the application number of 202011192001.2 and the title of "sperm liquefaction method, quality detection method and quality analyzer, storage medium" and the application number submitted on October 30, 2020. 202011194860.5 and titled "A sperm quality analyzer, detection method, and computer-readable storage medium" are priority documents, the entire contents of which are incorporated in the present disclosure by reference.

technical field

The present disclosure relates to the technical field of medical detection, and in particular, to a multifunctional sperm quality analyzer, a sperm quality detection method based on the multifunctional sperm quality analyzer, and a computer-readable storage medium.

Background technique

Sperm quality analysis is an important means of evaluating male fertility, and it is also the experimental basis for the diagnosis of andrological diseases and the observation of curative effects. Therefore, sperm quality analysis has very important guiding significance for medical clinical and scientific research projects. Among them, the sperm image is identified and tracked by the computer image processing function to accurately calculate and record the number, color, static characteristics and movement trajectory of the sperm, so as to obtain the dynamic and static parameters of the sperm, which is to complete the sperm quality detection and analysis. core link.

At present, the multifunctional sperm quality analyzers on the market generally only include the execution links such as sample injection, sampling, detection, and sample discarding. It has the detection function of a single item such as sperm color, sperm PH value, sperm count, etc.; it can not meet the actual detection needs of simultaneous multi-item detection, and it is easy to increase the cost of instrument purchase and use, and reduce the detection efficiency.

The above information disclosed in this Background section is only for enhancement of understanding of the background of the technology described in this article and therefore it may contain certain information that does not form part of the already known in this country to a person of ordinary skill in the art known prior art.

SUMMARY OF THE INVENTION

The main technical problem solved by the present disclosure is to provide a multifunctional sperm quality analyzer, a sperm quality detection method based on the multifunctional sperm quality analyzer, and a computer-readable storage medium, so as to realize multi-item detection of sperm and improve detection efficiency. Purpose.

According to an aspect of the embodiments of the present disclosure, a multifunctional sperm quality analyzer is provided, including a sampling mechanism, a controller, a liquefaction processing mechanism, and a detection processing mechanism; the liquefaction processing mechanism is provided with an incubation heating component, and the liquefaction processing mechanism The processing mechanism can be controlled by the controller, so that the sample in the sample container is heated and liquefied by the incubation heating part; the sampling mechanism is provided with a sampling needle, and the sampling mechanism can accept the control of the controller. control, aspirating the samples in the sample container through the sampling, and performing liquefaction detection; the detection processing mechanism is composed of a reaction processing mechanism and/or a microscope inspection processing mechanism; the reaction processing mechanism is provided with a reaction container and a first imaging part, the reaction processing mechanism is used to capture images of the samples before and after the reaction reagent is injected into the reaction container through the first imaging part; the microscopic inspection processing mechanism is provided with a microscope and a second Two image capturing components, the microscope inspection processing mechanism is used for focusing the sample on the detection card through the microscope, and capturing images on the sample on the detection card through the second imaging component; the control The controller is signal-connected with the liquefaction processing mechanism, the sampling mechanism, the reaction processing mechanism and/or the microscope inspection processing mechanism, and the controller performs a signal connection to the liquefaction processing mechanism, the sampling mechanism, the reaction processing mechanism The processing mechanism and/or the microscopic inspection processing mechanism performs timing control.

Optionally, the liquefaction processing mechanism further includes a sample carrying platform and a sample injection walking driving member; wherein, the sample carrying platform is used to place the sample container containing the semen sample; the incubation and heating component is provided on the on the sample carrying table, for heating the sample container, so as to keep the temperature of the semen sample in the sample container within a preset range; the sample feeding driving member is used for driving the sample carrying table reciprocating, so as to mix the semen sample in the sample container evenly during the process of transporting the sample container by the sample carrying table.

Optionally, the walking track of the sample injection walking drive assembly has a sampling position, so that when the sample carrying platform transports the mixed semen sample to the sampling position, the sampling mechanism can extract the sample from the sample. The semen sample is drawn into the container.

Optionally, the sampling mechanism further includes a sampling driving member and a sampling walking driving member; wherein, the sampling driving member is connected with the sampling needle through a liquid pipeline, and after the preparation of the semen sample is completed, the sampling needle is driven to suck and suck. The semen sample is discharged to realize the sample collection and sample addition functions of the sampling mechanism.

Optionally, the sampling driver provides a continuously changing positive and negative pressure effect, so that the sampling needle repeatedly sucks and discharges the semen sample, so as to achieve the effect of mixing the semen sample uniformly.

Optionally, the sampling mechanism further includes a viscosity detector for detecting the viscosity of the semen sample.

Optionally, the viscosity detector is a pressure sensor, and the pressure sensor is installed at the end of the sampling needle, so as to finally realize the viscosity judgment of the semen sample by detecting the internal pressure value of the sampling needle.

Optionally, the multifunctional sperm quality analyzer further includes a card loading mechanism, and the controller is signal-connected with the card loading mechanism and used to control the execution action of the card loading mechanism.

Optionally, the upper card mechanism includes a card compartment and an upper card driver; wherein, the card compartment is used for storing and accommodating the unused detection card; the upper card driver is used to remove the detection card The card compartment is in the second sample loading position.

Optionally, a detection card incubator is installed on the card compartment, and the detection card incubator is used to heat the detection card in the card compartment, so that the temperature of the detection card can be maintained at a preset value. within the temperature range.

Optionally, the reaction processing mechanism further includes a camera, a sample adding pipeline and a sample adding driver; wherein, the camera can take pictures of the samples in the reaction container; the reaction container is arranged in the first sample adding position, so that the sampling mechanism can add a semen sample to the reaction container at the first sample adding position; one end of the sample adding pipeline extends into the reaction container, and the sample adding driver is connected to the reaction container. One end of the sample adding pipeline that is far away from the reaction container is connected; the sample adding driving part is used to drive the sample adding pipeline to add a reaction reagent into the reaction container, so that the semen sample can react with the reaction container The reagents are mixed to form a mixed sample.

Optionally, the microscopic examination processing mechanism further includes a detection card conveying mechanism and a microscopic examination incubating assembly, and the second imaging component is a camera; wherein, the detection card conveying mechanism is used to transfer the detection card from the The second sample adding position is transferred to the microscope inspection position; the microscope inspection incubation component is arranged on the test card conveying mechanism, and is used for heating the test card, so as to keep the temperature of the test card within a preset range; The microscope is arranged at the microscope inspection position, and is used for focusing the detection card in the microscope inspection position; the camera is arranged at the eyepiece of the microscope, and is used for the focusing area of the microscope. Shooting of video or image, so as to provide observation conditions for the activity state of sperm in the semen sample on the detection card.

According to another aspect of the embodiments of the present disclosure, a method for detecting sperm quality based on the multifunctional sperm quality analyzer is also provided. The controller uses a preset detection method to detect the liquefaction processing mechanism, the sampling mechanism, and the reaction processing mechanism. and/or the microscopy processing mechanism performs sequence control, and the detection method includes: acquiring a liquefaction list and a detection list, wherein the liquefaction list and the detection list each include one or more sample information; triggering execution of a sample according to the liquefaction list liquefaction processing thread, obtain the first information, and add the sample information after the liquefaction processing to the detection list; trigger and execute the reaction processing thread of the sample according to the detection list to obtain the third information, and/or, according to The detection list triggers execution of the microscopic inspection processing thread of the sample to obtain fourth information; and outputs the first information, the third information and/or the fourth information.

Optionally, the triggering of the liquefaction processing thread that executes the sample according to the liquefaction list, and obtaining the first information includes the following steps: selecting one piece of the sample information from the liquefaction list, and triggering the liquefaction processing thread from an idle state. Entering the liquefaction execution state to start the liquefaction process for the sample corresponding to the selected sample information; the timing step, timing the liquefaction process of the sample to obtain the first time; the liquefaction detection step, detecting whether the first time reaches the second time time, when the first time reaches the second time, the liquefaction degree detection is performed; the classification and disposal step is performed according to the liquefaction degree detection result, if the liquefaction degree detection result is qualified, the liquefaction result is output, if the liquefaction degree detection result is qualified If the degree detection result is unqualified, it is judged whether the actual liquefaction time of the current sample reaches the third time, and if the actual liquefaction time reaches the third time, the liquefaction is stopped and a prompt message indicating the abnormal liquefaction of the current sample is output, If the actual liquefaction time does not reach the third time, continue to perform the timing step and the liquefaction detection step, the third time is the preset maximum time allowed for liquefaction, and the second time is less than the third time; determine whether the actions in the liquefaction execution state are all completed; if so, generate the liquefaction information of the sample; if otherwise, generate the first fault information; obtain the first fault information by using the liquefaction information or the first fault information a message.

Optionally, the classifying and processing step includes: comparing the liquefaction degree detection result with a preset threshold; if the liquefaction degree detection result is less than or equal to the preset threshold, outputting the liquefaction result; if the liquefaction degree detection result is less than or equal to the preset threshold If the liquefaction degree detection result is greater than the preset threshold, determine whether the actual liquefaction time reaches the third time; if the actual liquefaction time does not reach the third time, extend the second time to obtain a new the second time, and compare the first time with the new second time in continuing the liquefaction detection step; if the actual liquefaction time reaches the third time, output the current sample The prompt information of the liquefaction abnormality.

Optionally, the classifying and processing step includes: comparing the liquefaction degree detection result with a preset threshold; if the liquefaction degree detection result is less than or equal to the preset threshold, outputting the liquefaction result; If the liquefaction degree detection result is greater than the preset threshold, it is determined whether the actual liquefaction time reaches the third time; if the actual liquefaction time does not reach the third time, the first time is cleared to zero , and start timing from zero in the continuous execution of the timing step; if the actual liquefaction time reaches the third time, the prompt information of the abnormal liquefaction of the current sample is output.

Optionally, the liquefaction degree detection result is the viscosity value of the semen sample.

Optionally, the detecting the degree of liquefaction includes driving a sampling needle to be inserted into the sample container corresponding to the sample information to draw the sample, and detecting the viscosity value of the sample by sensing the internal pressure of the sampling needle.

Optionally, the triggering the execution of the reaction processing thread of the sample according to the detection list to obtain the third information includes: selecting one piece of the sample information from the detection list, and triggering the reaction processing thread from an idle state. Enter the reaction execution state; in the reaction execution state, drive a sampling needle to draw the sample from the sample container corresponding to the sample information and add the sample to a reaction container, and take pictures to obtain the sample in the reaction container. The first image of the sample, then inject the reaction reagent into the reaction container and take a second image of the sample in the reaction container; determine whether the actions in the reaction execution state are all completed, and if so, generate all the The reaction information of the sample is generated, if otherwise, second fault information is generated; the third information is obtained by using the reaction information or the second fault information.

Optionally, the triggering the execution of the microscopic inspection processing thread of the sample according to the detection list to obtain the fourth information includes: selecting one piece of the sample information from the detection list, and triggering the microscopic inspection processing thread from The idle state enters the microscopic inspection execution state; in the microscopic inspection execution state, drive a sampling needle to suck the sample from the sample container corresponding to the sample information and add the sample to a detection card, and transfer the detection card to the Microscopically check the position and drive a microscope to focus on the detection card, take a third image and/or a first video of the sample on the detection card; determine whether all the actions in the execution state of the microscopic examination are completed , if yes, generate the microscopic inspection information of the sample, if otherwise, generate third fault information; obtain the fourth information by using the microscopic inspection information or the third fault information.

Optionally, the triggering the execution of the microscopic inspection processing thread of the sample according to the detection list to obtain the fourth information includes: selecting one piece of the sample information from the detection list, and triggering the microscopic inspection processing thread from The idle state enters the microscopic inspection execution state; in the microscopic inspection execution state, the sampling needle is driven to suck the sample from the sample container corresponding to the sample information, add the sample to a detection card, and transfer the sample The detection card is moved to the microscope inspection position, and a microscope is driven to focus on the detection card, and a third image and/or a first video of the sample on the detection card is captured; Whether the actions are all completed, if so, generate the microscopic inspection information of the sample, if otherwise, generate the third fault information; use the microscopic inspection information or the third fault information to obtain the fourth information.

Optionally, when it is necessary to perform the reaction processing and the microscopic examination processing on the sample at the same time, check the status of the reaction processing thread and the microscopic examination processing thread, and determine the reaction processing thread and the microscopic examination processing thread. Whether the processing threads are idle at the same time, if so, trigger the reaction processing thread to enter the reaction execution state from the idle state, and trigger the mirror inspection processing thread to enter the mirror inspection execution state from the idle state, and then execute the The reaction processing thread and the mirror inspection processing thread, if otherwise, interrupt triggering until both the reaction processing thread and the mirror inspection processing thread are idle.

Optionally, after obtaining the first information, the liquefaction processing thread is restored to enter the idle state.

Optionally, after obtaining the third information, the reaction processing thread is restored to enter the idle state.

Optionally, after obtaining the fourth information, the mirror inspection processing thread is restored to enter the idle state.

Optionally, before acquiring the liquefaction list and the detection list, the method further includes: in response to a user's input instruction, classifying the sample information of one or more samples to be detected into the liquefaction list or the detection list list.

According to another aspect of the embodiments of the present disclosure, there is also provided a multifunctional sperm quality analyzer, comprising: a memory for storing a program; and a processor for implementing any one of the methods by executing the program stored in the memory the described detection method.

According to another aspect of the embodiments of the present disclosure, there is also provided a computer-readable storage medium, including a program that can be executed by a processor to implement any one of the detection methods.

The beneficial effects of the present disclosure are:

According to the multifunctional sperm quality analyzer of the above-mentioned embodiment, by integrating the liquefaction function and the detection function into one, multiple operation items such as sample liquefaction processing, liquefaction degree detection, and sample detection processing can be realized, which can not only improve the detection efficiency, and can meet the actual needs in the detection process.

Description of drawings

The accompanying drawings that constitute a part of the present disclosure are used to provide further understanding of the present disclosure, and the exemplary embodiments of the present disclosure and their descriptions are used to explain the present disclosure and do not constitute an improper limitation of the present disclosure. In the attached image:

1 is a schematic diagram of the system principle of a multifunctional sperm quality analyzer provided in Embodiment 1;

2 is a schematic structural reference diagram of a multifunctional sperm quality analyzer provided in Embodiment 1;

Fig. 3 is the flow chart of a kind of sperm quality detection method provided in embodiment 2;

Fig. 4 is the flow chart of the liquefaction processing thread of a kind of sperm quality detection method provided in embodiment 2;

FIG. 5 is a flowchart (1) of the classification and disposal steps in the liquefaction processing thread in FIG. 4;

FIG. 6 is a flowchart (2) of the classification and disposal steps in the liquefaction processing thread in FIG. 4;

7 is a flowchart of a reaction processing thread of a sperm quality detection method provided in Embodiment 2;

8 is a flowchart of a microscopic examination processing thread of a sperm quality detection method provided in Embodiment 2;

9 is a flow chart of simultaneously executing a reaction processing thread and a microscopic examination processing thread in a method for detecting sperm quality provided in Embodiment 2;

10 is a flowchart of a method for detecting sperm quality in Embodiment 4;

11 is a schematic structural diagram of a multifunctional sperm quality analyzer in Embodiment 5;

FIG. 12 and FIG. 13 are respectively partial structural schematic diagrams of the reaction processing mechanism in FIG. 2;

Fig. 14 is the structural assembly schematic diagram of the sampling mechanism in Fig. 2;

Fig. 15 is the partial structure assembly schematic diagram of the sampling needle part in Fig. 14;

Fig. 16 is a schematic diagram (1) of the structural assembly of the liquefaction processing mechanism in Fig. 2;

Fig. 17 is a schematic diagram (2) of the structural assembly of the liquefaction processing mechanism in Fig. 2;

Figure 18 is a schematic diagram of the internal structure of the liquefaction processing mechanism in Figure 2;

FIG. 19 is a schematic structural diagram of the microscope inspection processing structure in FIG. 2;

Fig. 20 is the structural assembly schematic diagram of the detection card conveying mechanism in Fig. 2;

Fig. 21 is the structural representation of the detection card base in Fig. 2;

Fig. 22 is a schematic diagram (1) of the structural assembly of the upper card mechanism in Fig. 2;

Fig. 23 is the structural assembly schematic diagram (2) of the upper card mechanism in Fig. 2;

FIG. 24 is a schematic diagram (3) of the structure and assembly of the upper card mechanism in FIG. 2 .

Wherein, the above-mentioned drawings include the following reference signs:

11. Sampling mechanism; 12. Reaction processing mechanism; 13. Microscopic inspection processing mechanism; 14. Controller; 15. Liquefaction processing mechanism; 16. Card loading mechanism; 41. Memory; 42. Processor; Parts; 210, sampling drive; 220, sampling travel drive; 221, gantry frame; 222, first guide rail; 223, sampling base; 224, second guide rail; 225, first travel drive; 226 230, sampling needle; 240, viscosity detector; 310, reaction vessel; 320, sample adding pipeline; 330, cleaning pipeline; 340, waste liquid collection pipeline; 410, sample carrying platform; 411, carrying base; 412, carrying cover plate; 413, container hole position; 420, incubation heating part; 430, sample injection travel drive part; 431, base; 432, drive motor; 433, guide rail; 434, Transmission assembly; 440, load cell; 500, test card conveying mechanism; 510, test card base; 520, test card driver; 521, test card guide platform; 522, test card driver; 530, microscope incubator ;540, discarding card drive; 541, discarding card motor; 542, discarding card baffle; 543, discarding card guide plate; 610, camera; 650, objective lens; 710, card bin; 720, detection card incubator; 730 731, screw motor; 732, positioning seat; 733, claw; 734, upper stop pin; 735, lower stop pin; 736, upper card bracket; 737, limit guide rail; 738 , shaft.

Detailed ways

The present disclosure will be further described in detail below through specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments have used associated similar element numbers. In the following embodiments, many details are described so that the present disclosure can be better understood. However, those skilled in the art will readily recognize that some of the features may be omitted under different circumstances, or may be replaced by other elements, materials, and methods. In some cases, some operations related to the present disclosure are not shown or described in the specification, in order to avoid the core part of the present disclosure being overwhelmed by excessive description, and for those skilled in the art, these are described in detail. The relevant operations are not necessary, and they can fully understand the relevant operations according to the descriptions in the specification and general technical knowledge in the field.

Additionally, the features, acts, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can also be exchanged or adjusted in order in a manner obvious to those skilled in the art. Therefore, the various sequences in the specification and drawings are only for the purpose of clearly describing a certain embodiment and are not meant to be a necessary order unless otherwise stated, a certain order must be followed.

The serial numbers themselves, such as "first", "second", etc., for the components herein are only used to distinguish the described objects, and do not have any order or technical meaning. The "connection" and "connection" mentioned in the present disclosure, unless otherwise specified, include both direct and indirect connections (connections).

The physical and chemical properties of semen usually include semen color, PH value, viscosity, volume, liquefaction time and other items; among them, normal semen should be gray or milky white viscous liquid, if the color of semen is yellow-green or red, it may be caused by the prostate gland. and seminal vesicle non-specific inflammation, germline tuberculosis, tumor or stone; normal semen pH value is 7.2-8.0, semen too acid (if less than 7.0) may be caused by blockage of ejaculatory duct, semen is too alkaline (if more than 7.0) 8.0) may be caused by inflammation of the seminal vesicles, so the change of PH value has a great influence on sperm motility and metabolism; the detection and analysis of semen color is very important for the differential diagnosis of diseases. At the same time, semen morphological testing usually includes items such as sperm motility and sperm count, among which sperm count is an important indicator to measure the ability of testes to produce sperm and the male reproductive tract. In addition, under normal circumstances, the male semen is in a liquefied state when it is just ejected from the body, and will solidify into a jelly or a clot in a short period of time. After 10-30 minutes, the semen will gradually liquefy into a watery liquid , This process is the liquefaction of semen, which is a normal physiological phenomenon. If the semen is still jelly after being discharged from the body for more than 30 minutes, it is a delayed liquefaction; if it still does not liquefy after more than 60 minutes, it is a pathological condition, usually caused by prostatitis, seminal vesiculitis, pathogenic microorganism infection, lack of trace elements, etc.

The sperm quality detection method and multifunctional sperm quality analyzer provided by the present disclosure include but are not limited to automatic liquefaction of semen, detection of physical and chemical properties such as semen color and pH value, and morphological detection of sperm count. By integrating the liquefaction mode and the detection mode into one, the liquefaction mode, the detection mode and the compatibility of the two modes can be implemented according to the user's needs or the condition of the semen sample, from the realization of sample liquefaction pretreatment, liquefaction degree detection, sampling, and sample addition. It can not only integrate all or most of the project links required to complete the sperm quality analysis to improve the detection efficiency, but also eliminate the influence of human operation differences by reducing manual intervention or operation. The accuracy of the test results creates conditions.

Example 1

Please refer to FIG. 1 , a multifunctional sperm quality analyzer is provided in this embodiment. The multifunctional sperm quality analyzer mainly includes a sampling mechanism 11 , a controller 14 , a liquefaction processing mechanism 15 , and a reaction processing mechanism 12 and/or a mirror The detection processing mechanisms formed by the detection processing mechanism 13 will be described separately below.

In this embodiment, the multifunctional sperm quality analyzer can inject one or more semen samples at a time, and the user can add the sample information of the semen samples to the preset liquefaction list or detection list by judging the state of the semen samples, so that Which samples need to be liquefied and which samples need to be tested directly.

In the present embodiment, the liquefaction processing mechanism 15 is provided with an incubation heating part 420, and the liquefaction processing mechanism 15 can accept the control of the controller 14, so that the incubating heating part 420 can process the sample container corresponding to each sample information in the liquefaction list. The sample inside is heated and liquefied, such as heating and heat preservation at 37°C, so as to simulate the internal environment, so as to speed up the liquefaction speed of the semen sample and realize the pretreatment operation of the sample.

It should be noted that semen liquefaction refers to the jelly-like semen that becomes thinner under the action of fibrinolytic enzymes after 10 to 20 minutes, and then the sperm can move fully; then, the liquefaction of semen samples is usually the same as that of the samples. The viscosity is related to the liquefaction time. The liquefaction time should be less than 30 minutes. If the liquefaction cannot be liquefied or exceeds 1 hour, the motility and survival rate of sperm will be affected, which can also be used as one of the indicators for the detection of sperm quality.

In the present embodiment, the sampling mechanism 11 is provided with a sampling needle 230, and the sampling mechanism 11 can be controlled by the controller 14. First, the sampling needle 230 can be used to perform sampling on the samples in the sample container corresponding to each sample information in the liquefaction list. Aspirate the sample and perform liquefaction detection; secondly, the sample corresponding to each sample information in the detection list can be aspirated, and the aspirated sample can be added to the reaction vessel and/or the detection card.

In this embodiment, the reaction processing mechanism 12 is provided with a reaction container 310 and a first imaging component 110 (such as a camera), then the reaction processing mechanism 12 can use the first imaging component 110 to inject the reaction reagent into the reaction container 310 before and after the reaction reagent. The samples were taken separately. Usually, the reaction treatment is mainly to detect the pH value of the semen sample. Adding a reaction reagent (PH reagent) to the semen sample can make the semen sample show different colors, so that the pH value can be determined by analyzing the color of the sample.

In this embodiment, the microscopic inspection processing mechanism 13 is provided with a microscope and a second imaging component (such as a camera), then the microscopic inspection processing mechanism 13 can focus the sample on the detection card through the microscope, and use the second imaging component to focus Take an image of the sample on the test card. The purpose of focusing microscopy is to magnify the visible area of the sample and perform morphological detection of semen by microscopic methods. The purpose of taking images is to obtain video and/or images of the focused area, which is convenient for analyzing the number or motility of sperm.

In this embodiment, the controller 14 is in signal connection with the liquefaction processing mechanism 15 , the sampling mechanism 11 , the reaction processing mechanism 12 and the microscopy processing mechanism 13 , and may be an integrated control circuit or a central processing unit. Then, the controller 14 performs time sequence control on the liquefaction processing mechanism 15 , the sampling mechanism 11 , the reaction processing mechanism 12 and the microscopic inspection processing mechanism 13 mainly through a preset detection method. For example: in the liquefaction link, the controller 14 controls the sampling mechanism 11 to absorb the sample in the sample container, and liquefies the result by detecting the viscosity of the sample; in the reaction processing link, the controller 14 controls the reaction processing mechanism 12 to perform the filling of the reaction reagent In the microscopic inspection processing link, the controller 14 controls the microscopic inspection processing mechanism 13 to automatically focus on the detection card, and video and/or focus on the focus area. or image capture.

In order to clearly understand the working principle of the multifunctional sperm quality analyzer, the specific structure of the multifunctional sperm quality analyzer will be described below through a specific embodiment.

Referring to FIG. 2, the multifunctional sperm quality analyzer includes a liquefaction processing mechanism 15, a sampling mechanism 11, an upper card mechanism 16, a reaction processing mechanism 12, and a microscopic inspection processing mechanism 13. Of course, the multifunctional sperm quality analyzer also includes a controller 14 (Fig. 2), the controller is in signal connection with these mechanisms and used to control the execution actions of the corresponding mechanisms. In addition, the first direction, the second direction and the third direction involved in FIG. 2 refer to the three directions naturally formed or the space formed by the three directions when the multifunctional sperm quality analyzer is in the environmental space A Cartesian coordinate system, wherein the first direction is the X-axis direction, the second direction is the Y-axis direction, and the third direction is the Z-axis direction.

In FIG. 2 , FIG. 16 , FIG. 17 and FIG. 18 , the liquefaction processing mechanism 15 is mainly composed of a sample carrying platform 410 , an incubating heating part 420 , a sample injection traveling drive part 430 , and the like. The sample holding table 410 is used for placing a sample container (such as reference numeral A) containing a semen sample. The sample holding table 410 is provided with a plurality of container holes 413 , and the sample container A is placed on the sample holding table through the container holes 413 410 on. The incubation heating component 420 (such as a heating plate, a heating film) is disposed on the sample carrying platform 410, and is mainly used for heating the sample container, so as to keep the temperature of the semen sample in the sample container within a preset range (such as 20°C-37°C). ℃), thereby facilitating the transformation of the semen sample from a coagulated state to a liquefied state, and promoting the liquefaction of the semen sample as soon as possible. The sample-injection driving member 430 is used to drive the sample carrying table 410 to reciprocate along the second direction through the guide rail, and the sample carrying table 410 generates a vibration effect by using the speed change effect produced by the sample carrying table 410 in the process of walking and changing of the walking direction. , so that the sample carrier 410 vibrates and mixes the semen samples in the sample container evenly during the process of transporting the sample container; at the same time, a sampling position is defined on the walking track of the sample injection walking drive assembly, so that the sample carrier 410 will mix the samples evenly. When the semen sample is delivered to the sampling position, the sampling mechanism 11 can draw the semen sample from the sample container.

In this embodiment, the sample carrying table 410 is mainly composed of a carrying base 411 and a carrying cover plate 412 . The carrying cover plate 412 is provided with a plurality of container holes 413 , and the sample container is placed on the carrying base through the container holes 413 . on seat 411. The incubation heating component 420 is disposed on the carrying base 411 , and the incubation heating component 420 adopts a heating film, and the heating film is attached to the side of the carrying base 411 close to the sample container A. The sample injection travel drive 430 is mainly composed of a base 431, a drive motor 432, a guide rail 433, and a transmission component 434 such as a belt drive mechanism and a screw drive mechanism. The carrying base 411 is slidably installed on the base 431 through the guide rail 433. The driving motor 432 is installed on the base 431 and is connected to the bearing base 411 through the transmission assembly 434; thus, the sample feeding driving member 430 is used to drive the sample bearing platform 410 to reciprocate in the second direction, and the sample bearing platform 410 is used to travel and The speed change effect generated in the process of changing the walking direction causes the sample carrying table 410 to generate a vibration effect, so that the sample carrying table 410 vibrates and mixes the semen sample in the sample container A during the process of conveying the sample container A. At the same time, a sampling position is defined along the running track of the sample carrying platform 410, and the sampling position is simultaneously located on the running track of the sampling mechanism 11, so that when the sample carrying platform 410 transports the mixed semen sample to be tested to the sampling position, the sampling mechanism 11 The semen sample to be tested can be collected from the sample container A. In one embodiment, a linear power output device such as an air cylinder can also be used to replace the drive motor 432, in which case the configuration of the transmission assembly 434 can be saved.

In one embodiment, referring to FIG. 18 , the liquefaction processing mechanism 15 further includes a load cell 440 , which is mounted on the bearing base 411 and corresponds to the container hole 413 one-to-one, so that the sample container A can be placed in the sample container A. After the sample carrying platform 410 is placed, the sample container A can be weighed, thereby creating conditions for finally obtaining the weight of the semen sample to be tested.

In FIGS. 2 , 14 and 15 , the sampling mechanism 11 includes a sampling driving member 210 , a sampling traveling driving member 220 and a sampling needle 230 . The sampling driver 210 is connected to the sampling needle 230 through a liquid pipeline, and after the preparation of the semen sample is completed, the sampling needle 230 can be driven to suck and discharge the semen sample, so as to realize the sample collection and sample addition functions of the sampling mechanism 11; The continuously changing positive and negative pressure effect provided by the component 210 enables the sampling needle 230 to repeatedly suck and discharge the semen sample, so as to achieve the effect of mixing the semen sample uniformly, so that the semen sample can meet the standard of subsequent detection. Among them, the sampling travel driver 220 includes a vertical guide rail and a horizontal guide rail, the vertical guide rail is slidably arranged on the horizontal guide rail and moves in the first direction, and the sampling needle 230 is fixed on the vertical guide rail and drives the sampling needle 230 for third-party operation. upward movement; when the vertical guide rail moves to the sampling position in the first direction, the sampling needle 230 is driven to be inserted into the sample container at the sampling position, so that the sampling driver 210 drives the sampling needle 230 to aspirate the sample in the sample container; When the vertical guide rail moves to the sample adding position in the first direction (eg, the first sample adding position of the reaction vessel, the second sample adding position of the test card), the sampling driver 210 drives the sampling needle 230 to discharge the sucked sample, thereby Load sample onto reaction vessel and test card. In addition, in order to facilitate the detection of the viscosity of the sample, the sampling mechanism 11 further includes a viscosity detector 240 to ensure that the sample drawn by the sampling mechanism 11 meets the relevant detection standards; The viscosity judgment of the semen sample is finally realized by detecting the internal pressure value of the sampling needle 230 .

In one embodiment, please refer to FIG. 14 and FIG. 15 , the sampling traveling drive member 220 includes a gantry frame 221, a first guide rail 222, a sampling base 223, a second guide rail 224, a first traveling drive member 225 and a second The traveling drive member 226; wherein, the lateral support arm of the gantry frame 221 is arranged along the first direction, and the liquefaction processing mechanism 15, the reaction processing mechanism 12, etc. are carried out by the structural space formed by the gantry frame 221 in the first direction. The assembly is assembled to form the structural assembly of the sperm quality analyzer. The first guide rail 222 is installed between the two longitudinal support arms of the gantry frame 221 along the first direction, the sampling base 223 is slidably installed on the first guide rail 222, and the main body of the first driving member 225 Then it is installed on the gantry frame 221, and the power output end of the first traveling driving member 225 is connected to the sampling base 223 (for example, a belt drive connection, a screw drive connection or a power component such as a cylinder is used for the first traveling driving member 225). , directly connected with the sampling base 223 ), so that the first guide rail 222 can be driven to reciprocate between the sampling position and the first sampling position. The second guide rail 224 is installed on the sampling base 223 along the third direction, the body of the second driving member 226 is installed on the sampling base 223, and the power output of the second driving member 226 is connected to the sampling needle 230 (eg, A belt drive connection, a screw drive connection, or when the second traveling driving member 226 adopts a power component such as an air cylinder, it is directly connected to the sampling needle 230), so that the sampling needle 230 can be driven in a third direction relative to the sampling base 223. walk.

In FIGS. 2 , 22 , 23 and 24 , the upper card mechanism 16 includes a card chamber 710 and an upper card driving member 730 . Among them, the card compartment 710 is used to store and accommodate unused test cards B; a test card incubator 720 can be installed on the card compartment 710, and the test card incubator 720 can be used to heat the test cards in the card compartment 710, so as to make the detection The temperature of the card can be maintained within a preset temperature range, thereby ensuring that after the semen sample is added to the test card, the temperature difference between the two will not adversely affect the semen sample and detection. Among them, the upper card driver 730 is used to move the detection card out of the card compartment 710 and in the second sample adding position of the detection card. By providing the function of automatically adding the detection card, the manual operation steps are minimized and conditions are created for improving the detection efficiency.

The upper card driving member 730 is mainly composed of a screw motor 731, a positioning seat 732, a shifting claw 733, an upper stop pin 734, a lower stop pin 735, an upper card bracket 736 and a limit guide rail 737; among them, the card chamber 710 is installed On the upper card bracket 736, the bottom of the card chamber 710 is provided with a card outlet channel, the main body of the screw motor 731 is installed on the upper card bracket 736, and the limit guide rail 737 is installed on the upper card bracket 736 along the second direction and is located in the upper card bracket 736. On the bottom end side of the cartridge 710, the positioning seat 732 is threadedly connected to the power shaft of the screw motor 731, and is slidably connected to the limit guide rail 737 at the same time. The center of gravity of the claw 733 is located at the lower end of the claw 733 , the upper stop pin 734 is installed on the positioning seat 732 and is located on the upper end side of the claw 733 in the card-out direction, and the lower stop pin 735 is installed on the positioning seat 732 and located on the claw 733 The lower end side in the opposite direction to the card ejecting direction. In the initial state, the shifting claw 733 rotates around the rotating shaft 738 under the action of gravity, and stops after contacting the lower stop pin 735. At this time, the top of the shifting claw 733 will protrude into the card chamber 710 through the card outlet channel, thereby It is in contact with the side of the detection card B away from the second sample adding position; when the screw motor 731 drives the positioning base 732 to move in the second direction towards the side where the second sample adding position is located, the shifting claw 733 can synchronously drive the detection card B moves, so that the test card B is finally pushed out of the card chamber 710 and enters the test card conveying mechanism 500 at the second sample adding position; when the screw motor 731 drives the positioning seat 732 to reset, the detection card in the card chamber 710 The pressing action will cause the shifting claw 733 to rotate around the rotating shaft 738, so that the top end of the shifting claw 733 is moved out of the cassette 710, and then abuts against the upper stop pin 734 until it returns to the original position, when the shifting claw 733 is released from the detection card After pressing, rotate again under the action of gravity, so that the top end enters the card chamber 710 through the card outlet channel, so as to offset the side of the next detection card away from the second sample adding position; Test card B is added to test card transport mechanism 500 .

Of course, in another case, there may be no card loading mechanism, and the user can manually load the card, and directly place the unused test card on the second sample adding position of the test card.

In FIG. 2 , FIG. 12 and FIG. 13 , the reaction processing mechanism 12 includes a reaction vessel 310 , a camera, a sample adding pipeline 320 and a sample adding driver. The reaction container 310 (such as a reaction cup) is made of a transparent material, so that the camera can take pictures of the sample in the reaction container 310; The sample adding position adds the semen sample into the reaction container 310 , so that the reaction container 310 can hold the semen sample. Wherein, one end of the sample adding pipeline extends into the reaction vessel, and the sample adding driver is connected to the end of the sample adding pipeline far from the reaction vessel; the sample adding driver can drive the sample adding pipeline to add the reaction reagent into the reaction vessel, so that the semen The sample is mixed with the reagents to form a mixed sample.

In one embodiment, as shown in FIG. 2 , FIG. 12 and FIG. 13 , the reaction processing mechanism 12 further includes a cleaning pipeline 330 , a waste liquid collection pipeline 340 , a cleaning driver (not shown in the figures) and a waste liquid driver One end of the cleaning pipeline 330 extends into the reaction vessel 310, and the cleaning driver is connected to the other end of the cleaning pipeline 330, so as to drive the The cleaning pipeline 330 adds cleaning liquid to the reaction container 310, so as to clean the residue in the reaction container 310; at the same time, one end of the waste liquid collection pipeline 340 is connected to the reaction container 310, and the waste liquid driving member is connected to the waste liquid collection The other end of the pipeline 340 is used to drive the waste liquid collection pipeline 340 to collect the cleaning waste liquid in the reaction container 310 after the cleaning of the reaction container 310 is completed, so as to realize the discharge and collection of the cleaning waste liquid, and then for the next sample It creates conditions for detection and avoids cross-contamination between samples or between samples and reaction reagents.

In FIGS. 2 , 19 , 20 and 21 , the microscopy processing mechanism 13 includes a detection card transport mechanism 500 , a microscopy incubator 530 , a microscope and a camera 610 . The test card conveying mechanism is used to transfer the test card B from the second sample adding position to the microscopic inspection position; the microscope incubator 530 is arranged on the test card conveying mechanism and is used to heat the test card B, so that the test card B The temperature, especially the temperature of the semen sample on the detection card B, is kept within a preset range (such as about 37°C), to create conditions for the effect of microscopic scanning. The microscope is set at the microscope inspection position, and is used to focus the detection card in the microscope inspection position through the automatic focusing assembly of the objective lens 650; the camera 610 is set at the eyepiece of the microscope, and is used to shoot video or images of the focus area of the microscope , so as to provide observation conditions for the detection of sperm activity in the semen sample on the card.

The test card conveying mechanism 500 includes a test card base 510 and a test card driver 520 that drives the test card base 510 to travel in the second direction; wherein the test card driver 520 is mainly composed of a test card guide platform 521 and a test card driver 522 , the test card guide platform 521 is arranged along the second direction, the test card base 510 is slidably installed on the test card guide platform 521, the body of the test card driver 522 is installed on the test card guide platform 521, and the power end of the test card driver 522 is installed on the test card guide platform 521. It is connected with the detection card base 510 (such as through a screw drive connection, a belt drive connection, etc.) to drive the detection card base 510 to walk in the second direction, and a second The sample adding position and the microscopic inspection position, the sampling position, the second sample adding position and the first sample adding position are arranged in sequence along the first direction, and the second sample adding position and the microscope inspection position are arranged in sequence along the second direction, so as to detect the card base. When the seat 510 travels to the second sample adding position, add the test card B to the test card base 510, and at the same time, when the sampling mechanism 11 travels to the second sample adding position, a part of the semen sample to be tested can be added to the test card base 510. on the detection card B, and then walk to the first sample adding position and add another part of the semen sample to be tested to the reaction processing mechanism 12 . The microscope incubator 530 is mounted on the detection card base 510 . In this embodiment, the microscope incubator 530 adopts a heating film, which is attached to the side of the detection card base 510 close to the detection card B, thereby helping to reduce the volume size of the detection card base 510 .

In one embodiment, please refer to FIG. 19 and FIG. 20 , the detection card conveying mechanism 500 further includes a card discarding drive member 540, which is mainly composed of a card discarding motor 541, a card discarding baffle plate 542 and a card discarding guide plate 543; A discarding card position is defined on the walking trajectory of the detection card base 510 , and the discarding card position can be located between the microscope inspection position and the second sample adding position, or the second sample adding position can be set in sequence along the walking trajectory of the detection card base 510 . , mirror inspection position, and card discarding position; the card discarding motor 541 is installed on the detection card guiding platform 521 and is adjacent to the discarding card position, and a card discarding baffle plate 542 is provided at the position where the detection card guiding platform 521 is located at the discarding card position. Through the hollow space that moves in the third direction, the discarding motor 541 and the discarding baffle 542 are connected by a gear + rack drive to drive the discarding baffle 542 to move in the third direction, while the discarding guide plate 543 is installed. On the test card guide platform 521 and on one side of the card discarding position. After the microscopic examination processing mechanism completes the microscopic examination and scanning of the semen sample to be tested, the detection card driver 522 drives the detection card base 510 to move to the discarding position, and one end of the detection card B on the detection card base 510 is just aligned with the discarding card guide. The plate 543 is aligned, and the other end is aligned with the card discarding shutter 542; at this time, the discarding motor 541 drives the discarding shutter 542 to protrude to the side where the detection card base 510 is located through the hollow position of the detection card guiding platform 521 , so that the corresponding end of the detection card B on the detection card base 510 is lifted up, and the detection card B is inclined and slid onto the discarding card guide plate 543, thereby completing the discarded card collection of the detection card B; conditions for automatic card registration.

In some other embodiments, the detection processing mechanism can also be only the reaction processing mechanism 12 or the microscopic inspection processing mechanism 13, so as to reduce the functional items of the multifunctional sperm quality analyzer, reduce the structural volume and occupied space, and achieve compactness Create conditions for type and special type sperm quality analyzers.

Embodiment 2

Based on the multifunctional sperm quality analyzer provided in the first embodiment, a sperm quality detection method is provided in this embodiment, and the detection method is mainly applied in the controller shown in FIG. 1 .

In this embodiment, please refer to FIG. 3 , the detection method includes steps S210-S250, which will be described separately below.

Step S210, acquiring a liquefaction list and a detection list. Both the liquefaction list and the detection list here include one or more sample information.

The multifunctional sperm quality analyzer can inject one or more semen samples at one time, some of which are in a jelly state, and these semen samples need to be liquefied first. Then, before the semen sample is injected, the state of the semen sample can be identified manually, the sample information of the semen sample that needs liquefaction is added to the liquefaction list, and the semen sample that does not need liquefaction is added to the detection list, so as to which samples need liquefaction processing, Which samples need to be tested directly provides conditions.

Step S220, triggering and executing the liquefaction processing thread of the sample according to the liquefaction list, obtaining the first information, and adding the sample information after the liquefaction processing ends to the detection list.

Since the semen samples corresponding to each sample information in the liquefaction list need to be liquefied, each sample information can be selected in sequence according to the sample order in the table, thereby triggering the execution of the liquefaction processing thread of the sample corresponding to the sample information; the obtained first information It can include liquefaction results including liquefaction time, viscosity value, or fault information including an error in an action in the liquefaction process. After semen liquefaction, it indicates that the semen sample meets the test standards required for subsequent reaction processing and microscopic examination processing, so the sample information after the liquefaction processing needs to be added to the test list.

Of course, if there is no sample information in the liquefaction list, the liquefaction processing thread cannot be triggered. Therefore, in the case that at least one piece of sample information exists in the liquefaction list in step S210, the controller may directly proceed to step S220.

Step S230, triggering a reaction processing thread for executing the sample according to the detection list to obtain third information.

Usually, the semen samples corresponding to each sample information in the detection list need to undergo reaction processing, so each sample information can be selected in turn according to the sample order in the table, thereby triggering the execution of the reaction processing thread of the sample corresponding to the sample information; the obtained third The information includes reaction information including adding reaction reagents, taking pictures of samples, or failure information including an error in an action in reaction processing.

Of course, if there is no sample information in the detection list, the reaction processing thread cannot be triggered. Therefore, in the case that there is at least one sample information in the detection list in step S210, the controller may directly proceed to step S230.

Step S240, triggering and executing the microscopic inspection processing thread of the sample according to the detection list to obtain fourth information.

Usually, the semen samples corresponding to each sample information in the detection list need to undergo microscopic examination processing, so each sample information can be selected in turn according to the sample order in the table, thereby triggering the execution of the microscopic examination processing thread of the sample corresponding to the sample information; the obtained The fourth information includes microscopic inspection information including adjusting the focus of the objective lens, capturing a sample video or image, or including fault information that an action is wrong in the microscopic inspection process.

Of course, if there is no sample information in the detection list, the microscopy processing thread cannot be triggered. Therefore, in the case that there is at least one sample information in the detection list in step S210, the controller may directly proceed to step S240.

Step S250, output the first information, the third information and the fourth information.

It should be noted that, the first information, the third information and the fourth information are all test information or fault information, then the output of these information can be displayed to the user through display, and can also be subjected to subsequent image analysis processing. In addition, since the liquefaction processing thread, the reaction processing thread and the mirror inspection processing thread are executed synchronously, the corresponding information is output only when any of the threads is triggered. For example, if only the reaction processing thread is triggered, then only the output third information.

In this embodiment, the reaction processing thread that triggers the execution of the sample according to the detection list to obtain the third information, and the microscopy processing thread that triggers the execution of the detection list to obtain the fourth information belongs to the detection processing thread that triggers the execution of the sample according to the detection list. , obtain the second information; that is: the detection processing thread that triggers the execution of the sample in the detection list includes the reaction processing thread and the microscopy processing thread, that is to say, the second information is obtained by using the third information and the fourth information; in some embodiments , according to the detection list triggering the execution of the detection processing of the sample, the formation may also be only a reaction processing thread or a microscopic inspection processing thread, and in this case, the second information is the third information or the fourth information.

In the embodiment, the above-mentioned step S220 mainly involves the process of triggering the execution of the liquefaction processing thread of the sample. Referring to FIG. 4 , this step may specifically include S221-S228, which are respectively described below.

Step S211 , select a sample information from the liquefaction list, and trigger the liquefaction processing thread to enter the liquefaction execution state from the idle state to start the liquefaction process for the sample corresponding to the selected sample information. It can be understood that when there is a plurality of sample information in the liquefaction list, the controller can select one sample information in sequence, or can randomly select one sample information.

Step S222, timing step, timing the liquefaction process of the sample to obtain the first time.

Step S223, the liquefaction detection step, detects whether the first time reaches the second time, and when the first time reaches the second time, performs liquefaction degree detection.

In a specific embodiment, the detection result of the degree of liquefaction is the viscosity value of the semen sample. In the liquefaction execution state, a sampling needle is driven to be inserted into the sample container corresponding to the sample information to draw the sample, and the sample is detected by sensing the internal pressure of the sampling needle. viscosity value.

More specifically, referring to FIG. 2 , the controller controls the sampling mechanism 11 to perform some actions, drives the sampling needle 230 to move in the first direction and the third direction through the sampling travel driver 220, and reaches the sampling position of the sample container A, and drives the sampling needle 230 to move in the first direction and the third direction. The sampling needle is inserted into the sample container A. Then, the sampling needle 230 is driven by the sampling driver 210 to suck the semen sample in the sample container A, and then the pressure value inside the sampling needle 230 is detected by the pressure sensor provided at the tail of the sampling needle 230, and the controller calculates the viscosity value of the sample. Next, the controller can judge the liquefaction time required for the sample according to the viscosity value of the sample.

Of course, when the controller determines that the viscosity value of the sample is greater than the test standard, it can control the sampling driver to drive the sampling to repeatedly aspirate and dispense the sample in the sample container, thereby rapidly liquefying the sample in the sample container.

Step S224: Execute the classification processing step according to the liquefaction degree detection. If the liquefaction degree detection result is qualified, output the liquefaction result. If the liquefaction degree detection result is unqualified, determine whether the actual liquefaction time of the current sample has reached the third time. When the actual liquefaction time reaches the third time, the liquefaction is stopped and a prompt message indicating that the liquefaction of the current sample is abnormal is output. If the actual liquefaction time of the current sample does not reach the third time, step S222 (the timing step) and step S223 (the liquefaction detection step) are continued. step).

It should be noted that the second time can be a preset detection time point or time period (such as 5min, 15min, 30min, 1h and other time nodes from the start of timing; or from the start of timing, a fixed time period of 10min , 30min, etc., and can be repeated one or more times), the third time should be the preset maximum time allowed for liquefaction (such as 1h), and the second time should be less than the third time.

In step S225, it is judged whether all the actions in the liquefaction execution state are completed, and if so, it goes to step S226, otherwise, it goes to step S227.

It should be noted that the actions in the liquefaction execution state at least include the action of driving the sampling needle, sucking the sample, detecting the internal pressure of the sampling needle, and calculating the viscosity value. Step S226 can be entered after all these actions are completed.

In step S226, since all the actions in the liquefaction execution state are completed, it indicates that the liquefaction process is completed, and liquefaction information of the sample can be generated at this time. The liquefaction information may include the viscosity value of the sample and the liquefaction time of the sample.

In step S227, since the actions in the liquefaction execution state are not all completed, it indicates that some links are faulty, and at this time, the first fault information can be generated. For example, if the action of detecting the internal pressure of the sampling needle cannot be performed, the first fault information that can be generated includes an error in detecting the pressure; if the semen sample cannot be liquefied, a prompt message of abnormal liquefaction is generated.

Step S228, obtaining the first information by using the liquefaction information or the first fault information.

In one embodiment, the above-mentioned step S224 mainly involves performing the classification and treatment process according to the detection of the liquefaction degree. Referring to FIG. 5 , this step specifically includes S2241-S2245, which are described as follows.

Step S2241, compare the liquefaction degree detection result with the preset threshold; if the liquefaction degree detection result is less than or equal to the preset threshold, execute step S2242; if the liquefaction degree detection result is greater than the preset threshold, execute step S2243.

Step S2242, output the liquefaction result.

Step S2243, determine whether the actual liquefaction time of the current sample reaches the third time, if the actual liquefaction time of the current sample does not reach the third time, then execute step S2244; if the actual liquefaction time of the current sample reaches the third time, execute step S2245 .

Step 2244: Extend the second time to obtain a new second time, and compare the first time with the new second time in the continuing step S223 (liquefaction detection step).

Step S2245, outputting prompt information of abnormal liquefaction of the current sample.

In this way, through the control of the second time, the second time is set as multiple time nodes from the timing step, so that the semen samples that have not been liquefied can be tested for the degree of liquefaction multiple times within the third time. The time is set to 5min. When the first time reaches 5min, the liquefaction degree detection is carried out. If the liquefaction degree detection result is unqualified, the second time is extended by 10min, so that the node to obtain a new second time is the 15th minute from the timing step. , when the first time reaches 15min, carry out the liquefaction degree detection; if the liquefaction degree detection result is still unqualified, continue to extend the second time and obtain a new second time, until the second time reaches the third time, carry out the final One-time liquefaction detection.

In another embodiment, in one embodiment, the above-mentioned step S224 mainly involves performing a classification treatment process according to the detection of the liquefaction degree, then referring to FIG.

Step S2246, compare the liquefaction degree detection result with the preset threshold; if the liquefaction degree detection result is less than or equal to the preset threshold, execute step S2247; if the liquefaction degree detection result is greater than the preset threshold, execute step S2248.

Step S2247, output the liquefaction result.

Step S2248, determine whether the actual liquefaction time of the current sample reaches the third time, if the actual liquefaction time of the current sample does not reach the third time, then execute step S2249; if the actual liquefaction time of the current sample reaches the third time, execute step S22410 .

Step 2249, clear the first time to zero, and start timing from zero in the execution of step S222 (timing step).

Step S22410, outputting prompt information of abnormal liquefaction of the current sample.

In this way, by controlling the second time, the second time is a fixed time period from the timing step, so that the liquefaction degree detection of the semen samples that have not yet been liquefied can be performed multiple times within the third time, which is equivalent to every other The second time (such as 5min, 10min, etc.) is to perform a liquefaction degree detection, and the actual liquefaction time is equivalent to the superposition of multiple second times. When the superimposed second time reaches the third time, the last time Liquefaction degree detection.

In this embodiment, the above-mentioned step S230 mainly involves the process of triggering the reaction processing thread for executing the sample. Referring to FIG. 7 , this step may specifically include S231-S236, which are described as follows.

Step S231, select a sample information from the detection list, and trigger the reaction processing thread to enter the reaction execution state from the idle state. It can be understood that when there are multiple pieces of sample information in the detection list, the controller can select one piece of sample information in sequence, or randomly select one piece of sample information.

Step S232, in the reaction execution state, drive a sampling needle to take samples from the sample container corresponding to the sample information and add the sample to a reaction container, take a first image of the sample in the reaction container, and then inject the sample into the reaction container. React reagents and take a second image of the sample in the reaction vessel.

In a specific embodiment, referring to FIG. 2 , the controller controls the sampling mechanism 11 and the reaction processing mechanism 12 to perform some actions, driving the sampling needle to suck the semen sample in the sample container A through the sampling driving member, and driving the sampling needle through the sampling driving member. It moves in the second direction and the third direction and reaches the first sample adding position of the reaction container, so as to add the drawn semen sample to the reaction container. Then, the controller drives the camera to take a picture of the sample in the reaction container to obtain the first image, and then drives the sample adding driver to inject the reaction reagent (such as pH reagent) into the reaction container, and the semen sample and reaction reagent in the reaction container are to be reacted. After the sufficient reaction, the camera is driven to take pictures of the samples in the reaction vessel again to capture and obtain a second image.

It can be understood that after capturing the first image and the second image, the controller can analyze the pH value of the sample according to these images.

In step S233, it is judged whether all the actions in the reaction execution state are completed.

It should be noted that the actions in the reaction execution state include at least the action of driving the sampling needle, the action of adding the sample, the action of taking pictures for the first time, the action of adding the reaction reagent, and the action of taking pictures for the second time. After all these actions are completed, step S234 can be entered. .

In step S234, since all the actions in the reaction execution state are completed, it indicates that the reaction processing is completed, and reaction information of the sample can be generated at this time. The reaction information may include the first image and the second image, and may also include the pH value of the sample.

In step S235, since the actions in the reaction execution state are not all completed, it indicates that some links are faulty, and at this time, second fault information can be generated. For example, if the action of adding the reaction reagent cannot be performed, the second fault information that may be generated includes an error in adding the reaction reagent.

In step S236, the third information is obtained by using the response information or the second fault information.

In the embodiment, the above-mentioned step S240 mainly involves the process of triggering the execution of the microscopic inspection processing thread of the sample. Referring to FIG. 8 , this step may specifically include S241-S246, which are described as follows.

Step S241, select a sample information from the detection list, and trigger the microscopy processing thread to enter the microscopy execution state from the idle state.

It can be understood that when there are multiple pieces of sample information in the microscopic examination processing list, the controller may select one piece of sample information sequentially, or may randomly select one piece of sample information.

Step S242, in the microscopic inspection execution state, drive a sampling needle to suck the sample from the sample container corresponding to the sample information and add the sample to a detection card, transfer the detection card to the microscopic inspection position, and drive a microscope to focus on the detection card, Shoot the third image and/or the first video of the sample on the test card.

In a specific embodiment, referring to FIG. 2 , the controller controls the sampling mechanism 11 and the microscopic inspection processing mechanism to perform some actions, driving the sampling needle to suck the semen sample in the sample container A through the sampling driving member, and driving the sampling needle through the sampling driving member It moves in the second direction and the third direction and reaches the second sample loading position of the test card (such as a glass slide), so as to add the sucked semen sample to the test card. Then, the detection card is transferred to the microscope inspection position through the inspection card conveying mechanism, and the microscope is driven to adjust the lens so as to focus the inspection card in the microscope inspection position. Next, the camera is driven to capture video and/or images of the focus area on the detection card from the eyepiece of the microscope, so as to capture a third image and a first video.

It can be understood that, after capturing the third image and the first video, the controller can analyze these videos or images to obtain the activity state and quantity of sperm in the semen sample on the detection card.

In step S243, it is judged whether all the actions in the microscopic inspection execution state are completed, and if so, it goes to step S244, otherwise, it goes to step S245.

It should be noted that the actions in the microscopic inspection execution state include at least the action of driving the sampling needle, the action of adding the sample, the action of transferring the detection card, the action of focusing the microscope, and the action of photographing. After all these actions are completed, step S244 can be entered.

In step S244, since all the actions in the microscopic examination execution state are completed, it indicates that the microscopic examination processing is successful, and the microscopic examination information of the sample can be generated at this time. The microscopic examination information may include the third image and the first video, and may also include sperm in the sample. activity status and quantity.

In step S245, since the actions in the microscopic inspection execution state are not all completed, it indicates that some links are faulty, and at this time, third fault information can be generated. For example, if the focusing action of the microscope cannot be performed, the third fault information that can be generated includes a focusing error of the microscope.

In step S246, the fourth information is obtained by using the microscopic inspection information or the third fault information.

In one embodiment, after any task thread is executed, the corresponding task thread is restored from the execution state to the idle state, so as to facilitate restarting the thread. Specifically, the controller restores the liquefaction processing thread to enter the idle state after obtaining the first information, and restores the detection processing thread to enter the idle state after obtaining the second information (for example, after obtaining the third information, the controller restores the reaction processing thread to enter the idle state, and after obtaining the third information, the controller restores the reaction processing thread to enter the idle state, After the fourth message, the mirror inspection processing thread is resumed and enters an idle state).

Of course, when fault information is included in the first information and the second information (ie, the third information and/or the fourth information), the controller may not restore the corresponding task thread immediately, but restore the corresponding task thread after the fault is cleared. The task thread continues to enter the execution state, thereby continuing to perform the action at the point where the failure occurred. Currently, in some cases, the corresponding task thread can be exited after the fault is cleared, so that the task thread is in an idle state.

In one embodiment, for the liquefaction processing thread, the reaction processing thread or the mirroring processing thread, before triggering the execution of any one of the task threads, the idle state/execution state of the task thread is checked, and only the task thread is idle state can only be triggered.

In a specific embodiment, when it is necessary to perform reaction processing and microscopic examination processing on the semen sample at the same time, the execution of the reaction processing thread and the microscopic examination processing thread can be triggered at the same time. For this process, please refer to steps S310-S340 in FIG. 9 , which are described respectively. as follows.

Step S310, check the status of the reaction processing thread and the mirror inspection processing thread.

In step S320, it is judged whether the reaction processing thread and the microscopy processing thread are idle at the same time, and if so, it goes to step S330, otherwise, it goes to step S340.

Step S330, triggering the reaction processing thread to enter the mirror inspection execution state from the idle state, and triggering the mirror inspection processing thread to enter the mirror inspection execution state from the idle state, and then executing the two threads respectively. For the execution process, reference may be made to steps S231-S236 in FIG. 7 and steps S241-S246 in FIG. 8 , which will not be repeated here.

Step S340, if otherwise, interrupt triggering until both threads are idle.

It should be noted that executing the reaction processing thread and the microscopy processing thread at the same time can reduce the waiting time in some cases, because at this time, the sampling needle can add samples to the reaction container and the detection card at the same time, so as to avoid the sampling needle performing suction multiple times. Sample action and sample addition action.

In one embodiment, before acquiring the liquefaction list and the detection list (that is, before step S210 in FIG. 3 ), a response step is further included. The sample information of the sample is classified into the liquefaction list or the detection list. That is to say, when a certain semen sample is injected, the liquefaction state of the semen sample is manually identified and judged. For the semen sample that does not meet the test standard, the corresponding sample information is directly added to the liquefaction list, so that the semen sample is liquefied first. Handling operations.

In one embodiment, after outputting the first information and the second information (ie, the third information and/or the fourth information) (ie, after the step S250 in FIG. 3 ), it further includes a display step and a fault clearing step: (1) in In the display step, the controller displays the first information and the second information (ie, the third information and/or the fourth information) for the user to view; (2) in the fault clearing step, the controller displays the first information, the second information When the information includes corresponding fault information, the fault is cleared according to a preset fault handling mechanism, and the fault handling mechanism may be a preset processing mechanism in the controller, such as program self-check or fault alarm. It can be understood that after the fault is cleared, it is possible to return to the faulty action to continue execution, or to completely exit the corresponding task thread and wait for the next trigger to execute the thread.

Embodiment 3

Based on the multifunctional sperm quality analyzer provided in the first embodiment, this embodiment provides a sperm liquefaction method. The sperm liquefaction detection method is mainly applied in the controller shown in FIG. 1 . In this way, only the The liquefaction processing mechanism 15 and the sampling mechanism 11 are retained to form a method dedicated to liquefying the semen sample; the specific steps can refer to steps 210 and 220 in the second embodiment; the details are as follows:

Step 410, the step of obtaining a liquefaction list, where the liquefaction list includes one or more sample information;

Step 420, triggering the liquefaction step, selecting one of the sample information from the liquefaction list, and triggering the liquefaction processing thread to enter the liquefaction execution state from the idle state to start the liquefaction process for the sample corresponding to the selected sample information;

Step 430, timing step, timing the liquefaction process of the sample to obtain the first time;

Step 440, a liquefaction detection step, to detect whether the first time reaches the second time, and when the first time reaches the second time, perform liquefaction degree detection;

Step 450: Perform the classification and processing step according to the liquefaction degree detection result. If the liquefaction degree detection result is qualified, output the liquefaction result. If the liquefaction degree detection result is unqualified, determine whether the actual liquefaction time of the current sample reaches the third time. If the actual liquefaction time of the current sample reaches the third time, the liquefaction is stopped. If the actual liquefaction time of the current sample does not reach the third time, continue to perform the timing step and the liquefaction detection step. The second time is less than the third time, and the third The time is the preset maximum time allowed for liquefaction.

Embodiment 4

Based on the multifunctional sperm quality analyzer provided in the first embodiment, a sperm quality detection method is provided in this embodiment, and the sperm quality detection method is mainly applied in the controller shown in FIG. 1 .

Referring to FIG. 10 , the detection method in this embodiment includes two parts, one is a UI control part (ie, human-computer interaction control) and a thread control part, the former part is formed with display content on the user interface.

Referring to FIG. 10 , the controller forms some trigger buttons on the user interface, and the user uses the trigger buttons to start the sample testing work of the multifunctional sperm quality analyzer. For each injected sample, the user interface prompts whether the liquefaction operation is required. If the user selects yes, the sample information of the sample is added to the liquefaction list, and if no, the sample information of the sample is added to the detection list.

10 , the controller obtains the liquefaction list and the detection list, triggers the liquefaction processing thread for executing the sample according to the liquefaction list, triggers the reaction processing thread for executing the sample according to the detection list, and triggers the microscopy processing thread for executing the sample according to the detection list. The three task threads can be executed in no particular order and can be triggered synchronously.

In case 1, when selecting a sample information from the liquefaction list and triggering the liquefaction processing thread of the sample, check whether the thread is in an idle state, if so, execute the liquefaction processing thread, otherwise, wait until the thread is idle. For the process of executing the liquefaction processing thread, reference may be made to steps S221-S228 in the second embodiment.

In case 2, when selecting a sample information from the detection list and triggering the reaction processing thread of the sample, check whether the thread is in an idle state, if so, execute the reaction processing thread, otherwise wait until the thread is idle. For the process of executing the liquefaction processing thread, reference may be made to steps S231-S236 in the second embodiment.

In case 3, when selecting a sample information from the detection list and triggering the microscopic inspection processing thread of the sample, check whether the thread is in an idle state, if so, execute the microscopic inspection processing thread, otherwise, wait until the thread is idle. For the process of executing the microscopic inspection processing thread, reference may be made to steps S241-S246 in the second embodiment.

Referring to Figure 10, after each task thread ends, the test information and fault information of the task thread will be obtained. For example, the first information will be obtained after triggering the liquefaction processing thread that executes the sample, and the second information will be obtained after triggering the reaction processing thread that executes the sample. information, the third information will be obtained after triggering the microscopic inspection processing thread that executes the sample, and then the controller can output the information.

Referring to FIG. 10 , when the test information and fault information are output to the user interface, these information are displayed on the user interface, so that the user can check them in time.

Embodiment 5

Based on the detection method provided in Embodiment 2 or Embodiment 3, this embodiment provides a multifunctional sperm quality analyzer.

Please refer to FIG. 11 , the multifunctional sperm quality analyzer includes a memory 41 and a processor 42 . The memory 41 is used for storing a program, and the program may be the program code corresponding to the detection method in the second embodiment, or the program code corresponding to the detection method in the third embodiment.

The processor 42 is connected to the memory 41 for executing the program stored in the memory 41 to implement the detection method. For the functions implemented by the processor 42, reference may be made to the controller in the second embodiment or the third embodiment, which will not be described in detail here.

Those skilled in the art can understand that all or part of the functions of the various methods in the foregoing embodiments may be implemented by means of hardware or by means of computer programs. When all or part of the functions in the above embodiments are implemented by means of a computer program, the program may be stored in a computer-readable storage medium, and the storage medium may include: read-only memory, random access memory, magnetic disk, optical disk, hard disk, etc. The computer executes the program to realize the above-mentioned functions. For example, the program is stored in the memory of the device, and when the program in the memory is executed by the processor, all or part of the above functions can be realized.

In addition, when all or part of the functions in the above-mentioned embodiments are realized by means of a computer program, the program can also be stored in a server, another computer, a magnetic disk, an optical disk, a flash disk or a mobile hard disk and other storage media, and saved by downloading or copying All or part of the functions in the above embodiments can be implemented when the program in the memory is executed by the processor.

The above uses specific examples to illustrate the present disclosure, which is only used to help the understanding of the present disclosure, and is not intended to limit the present disclosure. For those skilled in the art to which the present disclosure pertains, based on the idea of the present disclosure, several simple deductions, modifications or substitutions can also be made.

Claims (28)

1. A multifunctional sperm quality analyzer is characterized in that it includes a sampling mechanism, a controller, a liquefaction processing mechanism and a detection processing mechanism;

   The liquefaction processing mechanism is provided with an incubation heating component, and the liquefaction processing mechanism can be controlled by the controller, so that the sample in the sample container is heated and liquefied by the incubation heating component;

   The sampling mechanism is provided with a sampling needle, and the sampling mechanism can be controlled by the controller to aspirate the sample in the sample container through the sampling, and perform liquefaction detection;

   The detection processing mechanism is composed of a reaction processing mechanism and/or a microscopic inspection processing mechanism; the reaction processing mechanism is provided with a reaction container and a first imaging part, and the reaction processing mechanism is used to pass the first imaging part. The samples before and after the reaction reagent is injected into the reaction container are imaged respectively; the microscopic inspection processing mechanism is provided with a microscope and a second imaging component, and the microscopic inspection processing mechanism is used for the sample on the detection card through the microscope. performing focusing, and imaging the sample on the detection card by the second imaging component;

   The controller is signal-connected with the liquefaction processing mechanism, the sampling mechanism, the reaction processing mechanism and/or the microscopic inspection processing mechanism, and the controller is connected to the liquefaction processing mechanism, the sampling mechanism, The reaction processing mechanism and/or the microscopic inspection processing mechanism perform time sequence control.
2. The multifunctional sperm quality analyzer according to claim 1, wherein the liquefaction processing mechanism further comprises a sample carrying platform and a sample injection walking driving member; wherein, the sample carrying platform is used for placing the sample containing the semen sample. the sample container; the incubating heating component is disposed on the sample carrying table, and is used for heating the sample container, so as to keep the temperature of the semen sample in the sample container within a preset range; The sample-injection driving member is used to drive the sample carrying table to reciprocate, so as to mix the semen samples in the sample container evenly during the process of transporting the sample container by the sample carrying table.
3. The multifunctional sperm quality analyzer according to claim 2, wherein the walking track of the sample injection walking drive assembly has a sampling position, so that the sample carrying platform transports the mixed semen sample to the In the sampling position, the sampling mechanism can draw the semen sample from the sample container.
4. The multifunctional sperm quality analyzer according to claim 1, wherein the sampling mechanism further comprises a sampling driving member and a sampling walking driving member; wherein, the sampling driving member is connected with the sampling needle through a liquid pipeline, After the preparation of the semen sample is completed, the sampling needle is driven to suck and discharge the semen sample, so as to realize the sample collection and sample addition functions of the sampling mechanism.
5. The multifunctional sperm quality analyzer according to claim 4, wherein the sampling driver provides a continuously changing positive and negative pressure effect, so that the sampling needle repeatedly sucks and discharges the semen sample, so as to achieve the accurate detection of all the semen samples. Describe the effect of mixing a semen sample.
6. The multifunctional sperm quality analyzer according to claim 1, wherein the sampling mechanism further comprises a viscosity detector, and the viscosity detector is used to detect the viscosity of the semen sample.
7. The multifunctional sperm quality analyzer of claim 6, wherein the viscosity detector is a pressure sensor, and the pressure sensor is installed at the end of the sampling needle to detect the internal pressure of the sampling needle value to finally realize the viscosity judgment of the semen sample.
8. The multifunctional sperm quality analyzer according to claim 1, wherein the multifunctional sperm quality analyzer further comprises an upper card mechanism, and the controller is signal-connected with the upper card mechanism and used to control the The execution action of the above-mentioned card mechanism.
9. The multifunctional sperm quality analyzer according to claim 8, wherein the upper card mechanism comprises a card chamber and an upper card driving member; wherein, the card chamber is used for storing and accommodating the unused detection card; The upper card driving part is used to move the detection card out of the card compartment and place it in the second sample adding position.
10. The multifunctional sperm quality analyzer according to claim 9, wherein a detection card incubator is installed on the card chamber, and the detection card incubator is used to heat the detection in the card chamber card, so that the temperature of the detection card can be maintained within a preset temperature range.
11. The multifunctional sperm quality analyzer according to claim 1, wherein the reaction processing mechanism further comprises a camera, a sample adding pipeline and a sample adding driver; wherein, the camera is capable of monitoring the samples in the reaction vessel. The sample is photographed; the reaction container is arranged at the first sample addition position, so that the sampling mechanism can add a semen sample to the reaction container at the first sample addition position; one end of the sample addition pipeline extends into the reaction container, and the sample addition driver is connected to the end of the sample addition pipeline that is far away from the reaction container; the sample addition driver is used to drive the sample addition pipeline into the reaction container Reactive reagents are added such that the semen sample is mixed with the reactive reagents to form a mixed sample.
12. The multifunctional sperm quality analyzer according to claim 1, wherein the microscopic examination processing mechanism further comprises a detection card conveying mechanism and a microscopic examination incubating assembly, and the second imaging component is a camera; wherein, the The test card conveying mechanism is used for transferring the test card from the second sample adding position to the microscope inspection position; the microscope inspection incubating assembly is arranged on the test card conveying mechanism and is used for heating the test card to The temperature of the detection card is kept within a preset range; the microscope is arranged in the microscope inspection position, and is used for focusing the detection card in the microscope inspection position; the camera is arranged in the microscope At the eyepiece of the microscope, it is used to shoot video or images in the focus area of the microscope, so as to provide observation conditions for the activity state of sperm in the semen sample on the detection card.
13. A method for detecting sperm quality based on the multifunctional sperm quality analyzer according to any one of claims 1 to 12, characterized in that the controller controls the liquefaction processing mechanism, the sampling mechanism and the reaction processing mechanism through a preset detection method. And/or the microscopic inspection processing mechanism performs time sequence control, and the detection method includes:

    obtaining a liquefaction list and a detection list, wherein the liquefaction list and the detection list each include one or more sample information;

    Trigger the liquefaction processing thread for executing the sample according to the liquefaction list, obtain the first information, and add the sample information after the liquefaction process to the detection list;

    Triggering and executing the reaction processing thread of the sample according to the detection list to obtain third information, and/or triggering and executing the microscopic inspection processing thread of the sample according to the detection list to obtain fourth information;

    The first information, the third information and/or the fourth information are output.
14. The detection method according to claim 13, wherein the triggering and executing the liquefaction processing thread of the sample according to the liquefaction list, and obtaining the first information comprises the following steps;

    Selecting one piece of the sample information from the liquefaction list, and triggering the liquefaction processing thread to enter the liquefaction execution state from the idle state to start the liquefaction process for the sample corresponding to the selected sample information;

    The timing step is to time the liquefaction process of the sample to obtain the first time;

    The liquefaction detection step is to detect whether the first time reaches the second time, and when the first time reaches the second time, perform liquefaction degree detection;

    The classification and disposal step is performed according to the detection result of the degree of liquefaction. If the detection result of the degree of liquefaction is qualified, the liquefaction result is output. If the detection result of the degree of liquefaction is unqualified, it is judged whether the actual liquefaction time of the current sample has reached the third time, If the actual liquefaction time reaches the third time, stop liquefaction and output a prompt message indicating that the current sample is liquefied abnormally; if the actual liquefaction time does not reach the third time, continue to execute the timing step and the liquefaction detection step, the third time is a preset maximum time allowed for liquefaction, and the second time is less than the third time;

    Determine whether the actions in the liquefaction execution state are all completed;

    If so, generate the liquefaction information of the sample; if otherwise, generate the first fault information;

    The first information is obtained by using the liquefaction information or the first fault information.
15. The detection method according to claim 14, wherein the classification and treatment step comprises:

    comparing the detection result of the degree of liquefaction with a preset threshold;

    If the liquefaction degree detection result is less than or equal to the preset threshold, output the liquefaction result;

    If the liquefaction degree detection result is greater than the preset threshold, determine whether the actual liquefaction time reaches the third time;

    If the actual liquefaction time does not reach the third time, extend the second time to obtain a new second time, and compare the first time with the new second time in continuing to perform the liquefaction detection step two time comparisons;

    If the actual liquefaction time reaches the third time, the prompt information of the abnormal liquefaction of the current sample is output.
16. The detection method according to claim 14, wherein the classification and treatment step comprises:

    comparing the detection result of the degree of liquefaction with a preset threshold;

    If the liquefaction degree detection result is less than or equal to the preset threshold, output the liquefaction result;

    If the liquefaction degree detection result is greater than the preset threshold, determining whether the actual liquefaction time reaches the third time;

    If the actual liquefaction time does not reach the third time, the first time is reset to zero, and the time is started from zero in the continuous execution of the timing step;

    If the actual liquefaction time reaches the third time, the prompt information of the abnormal liquefaction of the current sample is output.
17. The detection method of claim 14, wherein the liquefaction degree detection result is the viscosity value of the semen sample.
18. The detection method according to claim 17, wherein the detecting the degree of liquefaction comprises driving a sampling needle to be inserted into the sample container corresponding to the sample information to draw the sample, and sensing the internal pressure of the sampling needle by sensing the pressure inside the sampling needle. The viscosity value of the sample is detected.
19. The detection method according to claim 13, wherein the triggering and executing the reaction processing thread of the sample according to the detection list to obtain the third information comprises:

    Select one of the sample information from the detection list, and trigger the reaction processing thread to enter the reaction execution state from the idle state;

    In the reaction execution state, drive a sampling needle to suck the sample from the sample container corresponding to the sample information and add the sample to a reaction container, and take a first image of the sample in the reaction container. , and then inject a reaction reagent into the reaction container and take a second image of the sample in the reaction container;

    Determine whether the actions in the reaction execution state are all completed, and if so, generate the reaction information of the sample, and if otherwise, generate the second fault information;

    The third information is obtained by using the reaction information or the second fault information.
20. The detection method according to claim 13, wherein the triggering and executing the microscopic inspection processing thread of the sample according to the detection list to obtain the fourth information comprises:

    Select one of the sample information from the detection list, and trigger the microscopy processing thread to enter the microscopy execution state from the idle state;

    In the microscopic inspection execution state, drive a sampling needle to suck the sample from the sample container corresponding to the sample information and add the sample to a detection card, transfer the detection card to the microscopic inspection position, and drive a microscope pair The detection card is focused, and a third image and/or a first video of the sample on the detection card is obtained by shooting;

    Determine whether the actions in the microscopic inspection execution state are all completed, and if so, generate the microscopic inspection information of the sample, and if otherwise, generate the third fault information;

    The fourth information is obtained by using the microscopic inspection information or the third fault information.
21. The detection method according to claim 19, wherein the triggering and executing the microscopic inspection processing thread of the sample according to the detection list to obtain the fourth information comprises:

    Select one of the sample information from the detection list, and trigger the microscopy processing thread to enter the microscopy execution state from the idle state;

    In the microscopic inspection execution state, drive the sampling needle to suck the sample from the sample container corresponding to the sample information and add the sample to a detection card, transfer the detection card to the microscopic inspection position and drive A microscope focuses the detection card, and captures a third image and/or a first video of the sample on the detection card;

    Determine whether the actions in the microscopic inspection execution state are all completed, and if so, generate the microscopic inspection information of the sample, and if otherwise, generate the third fault information;

    The fourth information is obtained by using the microscopic inspection information or the third fault information.
22. The detection method according to claim 21, wherein when it is necessary to perform the reaction processing and the microscopic examination processing on the sample at the same time, check the status of the reaction processing thread and the microscopic examination processing thread, and determine Whether the reaction processing thread and the mirror inspection processing thread are idle at the same time, if so, trigger the reaction processing thread to enter the reaction execution state from the idle state, and trigger the mirror inspection processing thread to enter the idle state at the same time The mirror inspection execution state is then executed, respectively, the reaction processing thread and the mirror inspection processing thread, if otherwise, the trigger is interrupted until both the reaction processing thread and the mirror inspection processing thread are idle.
23. The detection method according to claim 14, wherein the liquefaction processing thread is restored to enter the idle state after the first information is obtained.
24. The detection method according to claim 19, wherein the reaction processing thread is restored to enter the idle state after the third information is obtained.
25. The detection method according to claim 20, wherein after obtaining the fourth information, the mirror inspection processing thread is restored to enter the idle state.
26. The detection method according to claim 13, characterized in that before acquiring the liquefaction list and the detection list, the method further comprises: in response to a user's input instruction, classifying the sample information of one or more samples to be detected into a group Similar to the liquefaction list or the detection list.
27. A multifunctional sperm quality analyzer, characterized in that it includes:

    memory, for storing programs; and

    A processor, configured to implement the detection method according to any one of claims 13 to 26 by executing a program stored in the memory.
28. A computer-readable storage medium, characterized by comprising a program, which can be executed by a processor to implement the detection method according to any one of claims 13 to 26.

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