CN114441249A

CN114441249A - Sperm liquefying method, sperm quality detecting method, sperm quality analyzer and storage medium

Info

Publication number: CN114441249A
Application number: CN202011192001.2A
Authority: CN
Inventors: 王雪锋; 陈齐文; 许德鹏; 石义康; 周勤宇; 张裕剑
Original assignee: Shenzhen Reetoo Biotechnology Co Ltd
Current assignee: Shenzhen Reetoo Biotechnology Co Ltd
Priority date: 2020-10-30
Filing date: 2020-10-30
Publication date: 2022-05-06

Abstract

A sperm liquefying method, a sperm quality detecting method, a sperm quality analyzer and a storage medium; the quality detection method comprises the following steps: acquiring a liquefaction list and a detection list, wherein the liquefaction list and the detection list both comprise one or more sample information; triggering a liquefaction processing thread for executing the sample according to the liquefaction list to obtain first information, and adding the sample information after the liquefaction processing into the detection list; and starting to execute the detection processing thread of the sample according to the detection list to obtain second information, wherein the second information comprises the detection information of the sample. Through being compatible as an organic whole with liquefaction thread and detection thread, the user can freely select liquefaction thread or detection thread according to the condition of demand or semen sample to realize that multiple operation items such as liquefaction processing, liquefaction degree detection, the detection of sample are handled, both can improve detection efficiency, can satisfy the actual demand in the testing process again.

Description

Sperm liquefying method, sperm quality detecting method, sperm quality analyzer and storage medium

Technical Field

The invention relates to the technical field of medical detection, in particular to a sperm liquefying method, a sperm quality detecting method, a sperm quality analyzer and a storage medium.

Background

The sperm quality analysis is an important means for evaluating the male fertility and is also a test basis for diagnosis of andrological diseases and observation of curative effect, so the sperm quality analysis has very important guiding significance for medical clinical and scientific research projects. The method comprises the steps of identifying and tracking and calculating sperm images through a computer image processing function, accurately calculating and recording the quantity, color, static characteristics and motion tracks of the sperm, and obtaining various parameters of the sperm in a dynamic state and a static state, and is a core link for finishing sperm quality detection and analysis.

At present, a multifunctional sperm quality analyzer in the market generally only comprises execution links such as sample introduction, sampling, detection, sample abandoning and the like, and before the sample introduction link, pretreatment operations such as sperm sample liquefaction and the like are generally required to be manually carried out; moreover, many of them have only a single-item detection function such as sperm color, sperm pH, and sperm count; the method can not only meet the actual detection requirement of synchronously carrying out multi-item detection, but also easily increase the cost for purchasing and using the instrument and reduce the detection efficiency.

Disclosure of Invention

The invention mainly solves the technical problem of providing a sperm liquefying method, a sperm quality detecting method, a multifunctional sperm quality analyzer and a storage medium, so as to realize the purposes of multi-item detection of sperm and improvement of detection efficiency.

According to a first aspect, there is provided in one embodiment a sperm liquefaction process comprising:

a step of obtaining a liquefaction list, wherein the liquefaction list comprises one or more pieces of sample information;

a liquefaction triggering step, namely selecting one piece of sample information from the liquefaction list, and triggering the liquefaction processing thread to enter a liquefaction execution state from an idle state so as to start liquefaction processing on a sample corresponding to the selected sample information;

timing, namely timing the liquefaction process of the sample to obtain first time;

a liquefaction detection step of detecting whether the first time reaches a second time, and detecting a liquefaction degree when the first time reaches the second time;

and performing a classification treatment step according to the liquefaction degree detection result, outputting the liquefaction result if the liquefaction degree detection result is qualified, judging whether the actual liquefaction time of the current sample reaches a third time if the liquefaction degree detection result is unqualified, stopping liquefaction if the actual liquefaction time of the current sample reaches the third time, and continuing performing a timing step and a liquefaction detection step if the actual liquefaction time of the current sample does not reach the third time, wherein the second time is less than the third time, and the third time is the preset maximum time for allowing liquefaction.

According to a second aspect, an embodiment provides a sperm quality detection method comprising:

acquiring a liquefaction list and a detection list, wherein the liquefaction list and the detection list both comprise one or more sample information;

triggering a liquefaction processing thread for executing the sample according to the liquefaction list to obtain first information, and adding the sample information after the liquefaction processing into the detection list;

starting to execute a detection processing thread of the sample according to the detection list to obtain second information, wherein the second information comprises detection information of the sample;

wherein the triggering of a liquefaction processing thread executing a sample according to the liquefaction list comprises:

a liquefaction triggering step, namely selecting one piece of sample information from the liquefaction list, and triggering an idle state in the liquefaction processing thread to enter a liquefaction execution state so as to start liquefaction processing on a sample corresponding to the selected sample information;

performing a classification treatment step according to the liquefaction degree detection result, outputting the liquefaction result if the liquefaction degree detection result is qualified, judging whether the actual liquefaction time of the current sample reaches a third time if the liquefaction degree detection result is unqualified, stopping liquefaction and outputting prompt information indicating that the current sample is abnormal in liquefaction if the actual liquefaction time of the current sample reaches the third time, and continuing to perform a timing step and a liquefaction detection step if the actual liquefaction time of the current sample does not reach the third time, wherein the second time is less than the third time, and the third time is the preset maximum time for allowing liquefaction;

and obtaining first information according to the liquefaction result and/or the prompt message.

In one embodiment, the step of categorizing comprises:

comparing the liquefaction degree detection result with a preset threshold value;

if the liquefaction degree detection result is less than or equal to a preset threshold value, outputting a liquefaction result;

if the liquefaction degree detection result is larger than the preset threshold value, judging whether the actual liquefaction time of the current sample reaches a third time;

and if the actual liquefaction time of the current sample does not reach the third time, prolonging the second time to obtain a new second time, and comparing the first time with the new second time in the step of continuously executing liquefaction detection.

In one embodiment, the step of categorizing comprises:

and if the liquefaction time of the current sample does not reach the third time, clearing the first time, and starting timing from zero in the step of continuously executing timing.

In one embodiment, after stopping liquefaction, the method further comprises: and outputting prompt information indicating that the current sample is abnormal in liquefaction.

In one embodiment, the liquefaction degree test result is a viscosity value of the semen sample.

In one embodiment, the performing the liquefaction degree detection includes:

and driving a sampling needle to be inserted into a sample container corresponding to the sample information to suck the sample, and detecting the viscosity value of the sample by sensing the internal pressure of the sampling needle.

In an embodiment, the triggering, according to the detection list, a detection processing thread that executes a sample to obtain the second information includes:

triggering a reaction processing thread for executing the sample according to the detection list to obtain third information; and/or

Triggering a microscopic examination processing thread of the execution sample according to the detection list to obtain fourth information;

and obtaining the second information by using the third information and/or the fourth information.

According to a third aspect, an embodiment provides a multifunctional sperm quality analyzer comprising a liquefaction processing mechanism, a sampling mechanism, a detection processing mechanism, and a controller;

the liquefaction processing mechanism is provided with an incubation heating part and is used for heating and liquefying the samples in the sample containers corresponding to the sample information in the liquefaction list through the incubation heating part;

the sampling mechanism is provided with a sampling needle, is used for sucking samples in the sample container through the sampling and carrying out liquefaction detection, and is used for sucking samples corresponding to each piece of sample information in a detection list and adding the sucked samples into a reaction container and/or a detection card;

the detection processing mechanism is provided with a reaction container and a first image capturing component and is used for respectively capturing images of samples before and after a reaction reagent is injected into the reaction container through the first image capturing component; and/or the detection processing mechanism is provided with a microscope and a second image capturing component, and is used for focusing the sample on the detection card through the microscope and capturing the image of the sample on the detection card through the second image capturing component;

the controller is in signal connection with the liquefaction processing mechanism, the sampling mechanism and the detection processing mechanism, and is used for performing time sequence control on the liquefaction processing mechanism, the sampling mechanism and the detection mechanism by the method of the second aspect.

A computer readable storage medium comprising a program executable by a processor to implement the method of the second aspect.

The beneficial effect of this application is:

a sperm quality detection method according to the above embodiment, comprising: acquiring a liquefaction list and a detection list, wherein the liquefaction list and the detection list both comprise one or more pieces of sample information; triggering a liquefaction processing thread of the sample according to the liquefaction list to obtain first information, and adding the sample information after the liquefaction processing into the detection list; and starting to execute a detection processing thread of the sample according to the detection list to obtain second information, wherein the second information comprises the detection information of the sample. Through being compatible as an organic whole with liquefaction thread and detection thread, the user can freely select liquefaction thread or detection thread according to the condition of demand or semen sample to realize that multiple operation items such as liquefaction processing, liquefaction degree detection, the detection of sample are handled, both can improve detection efficiency, can satisfy the actual demand in the testing process again.

Drawings

FIG. 1 is a schematic block diagram of a multifunctional sperm cell mass analyzer system according to one embodiment;

FIG. 2 is a schematic view of a multifunctional sperm cell mass analyzer according to one embodiment;

FIG. 3 is a flow chart of a method of sperm quality detection as provided in example two;

FIG. 4 is a flowchart of a liquefaction processing thread of a sperm cell quality detection method as provided in example two;

FIG. 5 is a flow chart of the sort handling step in the liquefaction processing thread of FIG. 4;

FIG. 6 is a flowchart of the sort handling step in the liquefaction processing thread of FIG. 4;

FIG. 7 is a flowchart of a reaction processing thread of a sperm cell quality detection method as provided in example two;

FIG. 8 is a flowchart of a microscopic examination processing thread of the sperm quality detection method according to the second embodiment;

FIG. 9 is a flowchart illustrating a process of performing a reaction process and a microscopy process simultaneously according to a sperm cell quality detection method as provided in example two;

FIG. 10 is a flowchart of a sperm cell quality detection process according to the fourth embodiment;

fig. 11 is a schematic structural view of a multifunctional sperm cell mass analyzer of the fifth embodiment.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The semen physicochemical property detection generally comprises the items of semen color, PH value, viscosity, volume, liquefaction time and the like; wherein, the normal semen is gray or milky viscous liquid, if the semen is yellow green or red, the semen may be caused by nonspecific inflammation of prostate and seminal vesicle, tuberculosis of reproductive system, tumor or calculus; the pH value of normal semen is 7.2-8.0, the sperm peracid (for example, less than 7.0) is probably caused by the obstruction of ejaculatory ducts, and the sperm hyperalkali (for example, more than 8.0) is probably caused by inflammation of seminal vesicles, so the change of pH value has great influence on sperm motility and metabolism; the detection and analysis of the semen color have very important significance for the differential diagnosis of diseases. Meanwhile, the semen morphology detection generally includes items such as sperm motility and sperm quantity, wherein the sperm quantity is an important index for measuring the sperm generating capacity of the testis and the male genital tract. In addition, under normal conditions, the semen of the male is liquefied just after being ejected out of the body and can be solidified into jelly or clot in a short time, and after 10-30 minutes, the semen can be gradually liquefied into water sample liquid, and the process is the liquefaction of the semen, and the phenomenon belongs to normal physiological phenomena. If the semen is still in a jelly shape after being discharged out of the body for more than 30 minutes, the liquefaction is delayed; if the fluid is not liquefied after more than 60 minutes, the fluid is pathological, and is usually caused by prostatitis, seminal vesiculitis, pathogenic microorganism infection, lack of trace elements and the like.

The sperm quality detection method and the multifunctional sperm quality analyzer provided by the application have the related functions including but not limited to automatic liquefaction of semen, detection of physicochemical properties such as semen color and PH value, and morphological detection such as sperm quantity. By integrating the liquefaction mode and the detection mode, the liquefaction mode, the detection mode and the compatibility of the two modes can be executed according to the requirements of users or the conditions of semen samples, and from the realization of serial operation links such as liquefaction pretreatment, liquefaction degree detection, sampling, sample adding, detection and the like of the samples, all or most project links required by sperm quality analysis can be integrated, the detection efficiency is improved, the influence of manual operation difference factors can be eliminated by reducing manual intervention or operation, and conditions are created for ensuring the accuracy of detection results.

Example one

Referring to fig. 1, the present embodiment provides a multifunctional sperm quality analyzer, which mainly comprises a sampling mechanism 11, a controller 14, a liquefaction processing mechanism 15, and a detection processing mechanism composed of a reaction processing mechanism 12 and/or a microscopy processing mechanism 13, which are respectively described below.

In this embodiment, the multifunctional sperm quality analyzer can sample one or more semen samples at a time, and a user can add sample information of the semen samples to a preset liquefaction list or detection list by judging the state of the semen samples, thereby providing conditions for which samples need to be liquefied and which samples need to be directly detected.

In this embodiment, the liquefaction processing mechanism 15 is provided with an incubation heating component, and the liquefaction processing mechanism 15 can be controlled by the controller 14, so that the sample in the sample container corresponding to each sample information in the liquefaction list is heated and liquefied by the incubation heating component, for example, the heating and heat preservation at 37 ℃ are performed, so as to simulate the in vivo environment, accelerate the liquefaction speed of the semen sample, and realize the pretreatment operation of the sample.

The semen liquefaction means that the semen in the jelly form becomes thinner under the action of fibrinolytic enzyme after 10-20 minutes, and then the semen can move fully; the liquefaction of semen samples is usually related to the viscosity of the sample and the liquefaction time, the liquefaction time is less than 30 minutes, and if the semen samples cannot be liquefied or are liquefied for more than 1 hour, the sperm motility and the survival rate are influenced, so that the semen samples can also be used as one of indexes for realizing the sperm quality detection.

In this embodiment, the sampling mechanism 11 is provided with a sampling needle, and the sampling mechanism 11 can be controlled by the controller 14, and first, the sampling can be performed on the sample in the sample container corresponding to each sample information in the liquefaction list through sampling, and the liquefaction detection is performed; secondly, the sample corresponding to each sample information in the detection list can be sucked, and the sucked sample is added into the reaction container and/or the detection card.

In the present embodiment, the reaction processing mechanism 12 is provided with a reaction container and a first image capturing component (e.g., a camera), so that the reaction processing mechanism 12 can capture images of samples before and after injecting the reaction reagent into the reaction container through the first image capturing component. Generally, the reaction process is mainly to detect the PH of the semen sample, and a reagent (PH reagent) different from the reaction reagent is added to the semen sample to make the semen sample appear different colors, so as to determine the PH by analyzing the color of the sample.

In the present embodiment, the microscopy processing mechanism 13 is provided with a microscope and a second image capturing component (e.g., a camera), so that the microscopy processing mechanism 12 can focus the sample on the test card through the microscope and capture the sample on the test card through the second image capturing component. The objective of the focusing lens examination is to enlarge the visible area of the sample, and the morphological detection of the semen is carried out in a microscopic mode, and the purpose of shooting and image taking is to obtain a video and/or an image of the focused area, so that the quantity or the vitality state of the sperms can be analyzed conveniently.

In the present embodiment, the controller 14 is in signal connection with the liquefaction processing mechanism 15, the sampling mechanism 11, the reaction processing mechanism 12 and the microscopy processing mechanism 13, and may be an integrated control circuit or a central processing unit. Then, the controller 14 performs timing control of the liquefaction processing mechanism 15, the sampling mechanism 11, the reaction processing mechanism 12, and the microscopic processing mechanism 13 mainly by a preset detection method. Such as: in the liquefaction link, the controller 14 controls the sampling mechanism 11 to suck the sample in the sample container, and liquefies the result by detecting the viscosity of the sample; in the reaction processing link, the controller 14 controls the reaction processing mechanism 12 to perform the filling action of the reaction reagent, and photographs the sample in the reaction container before and after the filling of the reaction reagent; in the microscopy processing link, the controller 14 controls the microscopy processing mechanism 13 to automatically focus the detection card and take video and/or image shooting of the focused area.

In order to clearly understand the working principle of the multifunctional sperm quality analyzer, the following description will be made on the specific structure of the multifunctional sperm quality analyzer by a specific embodiment.

Referring to fig. 2, the multifunctional sperm quality analyzer comprises a liquefaction processing mechanism 15, a sampling mechanism 11, a card loading mechanism 16, a reaction processing mechanism 12 and a microscopy processing mechanism 13, and of course, the multifunctional sperm quality analyzer further comprises a controller 14 (not shown in fig. 2), which is in signal connection with the mechanisms and is used for controlling the execution action of the corresponding mechanisms. In addition, the first direction, the second direction and the third direction referred to in fig. 2 refer to three directions or a spatial rectangular coordinate system formed by three directions naturally formed when the multifunctional sperm mass analyzer is in an environmental space, wherein the first direction is an X-axis direction, the second direction is a Y-axis direction, and the third direction is a Z-axis direction.

In fig. 2, the liquefaction processing mechanism 15 is mainly composed of a sample holder, an incubation heating part, a sample injection traveling driving part, and the like. The sample bearing platform is used for placing a sample container (such as a reference sign A) containing a semen sample, the sample bearing platform is provided with a plurality of container hole sites, and the sample container is placed on the sample bearing platform through the container hole sites. The incubation heating part (such as a heating sheet and a heating film) is arranged on the sample bearing platform and is mainly used for heating the sample container so as to keep the temperature of the semen sample in the sample container within a preset range (such as 20-37 ℃), so that the semen sample can be conveniently converted into a liquefied state from a solidified state, and the semen sample is promoted to be liquefied as soon as possible. The sample introduction walking driving part is used for driving the sample bearing platform to reciprocate along a first direction through the guide rail, and the sample bearing platform generates a vibration effect by utilizing a speed change effect generated in the walking and walking direction changing processes of the sample bearing platform so as to uniformly vibrate the semen sample in the sample container in the process of conveying the sample container by the sample bearing platform; meanwhile, a sampling position is defined on the walking track of the sample introduction walking driving assembly, so that when the sample bearing platform conveys the uniformly mixed semen sample to the sampling position, the sampling mechanism 11 can suck the semen sample from the sample container.

In fig. 2, the sampling mechanism 11 includes a sampling driving member, a sampling travel driving member, and a sampling needle. The sampling driving part is connected with the sampling needle through a liquid pipeline, and after the semen sample is prepared, the sampling driving part can drive the sampling needle to suck and discharge the semen sample, so that the sample collection and sample adding functions of the sampling mechanism 11 are realized; and the continuous positive and negative pressure effect provided by the sampling driving part can be utilized to enable the sampling needle to repeatedly suck and discharge the semen sample so as to achieve the effect of uniformly mixing the semen sample, thereby enabling the semen sample to meet the standard of subsequent detection. The sampling walking driving part comprises a vertical guide rail and a transverse guide rail, the vertical guide rail is arranged on the transverse guide rail in a sliding mode and moves in a second direction, and a sampling needle is fixed on the vertical guide rail and drives the sampling needle to move in a third direction; when the vertical guide rail moves to the sampling position in the second direction, the sampling needle is driven to be inserted into the sample container at the sampling position, so that the sampling driving part drives the sampling needle to suck samples in the sample container; when the vertical guide rail moves to the sample adding position (such as the first sample adding position of the reaction container and the second sample adding position of the detection card) in the second direction, the sampling driving member drives the sampling needle to discharge the absorbed sample, so that the sample is added to the reaction container and the detection card. In addition, in order to facilitate the detection of the viscosity of the sample, the sampling mechanism 11 further comprises a viscosity detector, which ensures that the sample sucked by the sampling mechanism 11 meets the relevant detection standard; for example, the viscosity detector may employ a pressure sensor, which is installed at the end of the sampling needle, and finally realizes the viscosity judgment of the semen sample by detecting the internal pressure value of the sampling needle.

In fig. 2, the upper card mechanism 16 includes a card hopper and an upper card driver. The card bin is used for storing and accommodating unused detection cards; can install detection card incubation spare on the card storehouse, utilize detection card incubation spare to heat the detection card in the card storehouse, make the temperature that detects the card keep presetting the temperature range, and then guarantee that semen sample can not produce harmful effects to semen sample and detection because of the temperature difference between the two after the application of sample to detecting the card. The upper card driving part is used for moving the detection card out of the card bin and is positioned at the second sample adding position of the detection card, manual operation steps are reduced to the greatest extent by providing a function of automatically adding the detection card, and conditions are created for improving the detection efficiency.

In another case, the card loading mechanism may be omitted, and the user manually loads the card and directly places the unused test card on the second loading position of the test card.

In fig. 2, the reaction processing mechanism 12 includes a reaction container, a camera, a sample application pipeline, and a sample application driving member. Wherein, the reaction container (such as a reaction cup) is made of transparent material, so that a camera can photograph the sample in the reaction container; the reaction container is arranged at the first sample adding position, so that the sampling mechanism 11 can add the semen sample to the reaction container at the first sample adding position, and the effect of the reaction container for containing the semen sample is realized. One end of the sample adding pipeline extends into the reaction container, and the sample adding driving part is connected with one end of the sample adding pipeline far away from the reaction container; the application of sample driving piece can drive application of sample pipeline to adding reaction reagent in the reaction vessel to make semen sample and reaction reagent mix, thereby form mixed sample.

In FIG. 2, the microscopy processing mechanism 13 includes a detection card transport mechanism, a microscopy incubation assembly, a microscope and a camera. The detection card conveying mechanism is used for transferring the detection card from the second sample adding position to the microscopic detection position; the microscopic examination incubation component is arranged on the detection card conveying mechanism and used for heating the detection card so as to keep the temperature of the detection card, particularly the temperature of the semen sample on the detection card within a preset range (such as about 37 ℃), and create conditions for the microscopic examination scanning effect. The microscope is arranged at the microscopic examination position and used for focusing the detection card at the microscopic examination position through the automatic focusing assembly of the objective lens; the camera is arranged at the ocular lens of the microscope and is used for shooting videos or images of the focusing area of the microscope, so that observation conditions are provided for detecting the moving state of sperms in the semen sample on the card.

In other embodiments, the detection processing mechanism may also be only the reaction processing mechanism 12 or the microscopic processing mechanism 13, so as to reduce the functional items of the multifunctional sperm quality analyzer, reduce the volume and the occupied space of the structure, and create conditions for realizing a compact and dedicated sperm quality analyzer.

Example two

On the basis of the multifunctional sperm quality analyzer provided in the first embodiment, a sperm quality detection method is provided in this embodiment, and the detection method is mainly applied to the controller shown in fig. 1.

In the present embodiment, referring to fig. 3, the detection method includes steps S210-S250, which are described below respectively.

Step S210, a liquefaction list and a detection list are acquired. The liquefaction list and the detection list herein each include one or more pieces of sample information.

The multifunctional sperm quality analyzer can sample one or more semen samples at one time, wherein some semen samples are in a jelly state, and the semen samples need to be liquefied firstly at the moment. Therefore, before the semen sample is injected, the state of the semen sample can be artificially identified, the sample information of the semen sample needing to be liquefied is added into the liquefaction list, the semen sample not needing to be liquefied is added into the detection list, and conditions are provided for which samples need to be liquefied and which samples need to be directly detected.

Step S220, triggering a liquefaction processing thread for executing the sample according to the liquefaction list to obtain the first information, and adding the sample information after the liquefaction processing to the detection list.

Because the semen samples corresponding to the sample information in the liquefaction list need to be liquefied, each sample information can be sequentially selected according to the sample sequence in the list, and the liquefaction processing thread of the sample corresponding to the sample information is triggered and executed; the obtained first information may include liquefaction time, liquefaction results including viscosity values, or fault information including an error in some action in the liquefaction process. After the semen is liquefied, the semen sample is shown to reach the test standard required by the subsequent reaction treatment and microscopic examination treatment, so that the sample information after the liquefaction treatment needs to be added into the detection list.

Of course, if there is no sample information in the liquefaction list, the liquefaction processing thread cannot be triggered. Therefore, in the case that at least one sample information exists in the liquefaction list in step S210, the controller may directly proceed to step S220.

Step S230, triggering a reaction processing thread for executing the sample according to the detection list to obtain third information.

Generally, semen samples corresponding to each sample information in the detection list need to be subjected to reaction processing, so that each sample information can be sequentially selected according to the sample sequence in the table, and a reaction processing thread of the sample corresponding to the sample information is triggered to be executed; the obtained third information includes reaction information including the filling of a reaction reagent and the taking of a sample picture, or includes failure information that a certain action in the reaction process is wrong.

Of course, if there is no sample information in the test list, the reactive processing thread cannot be triggered. Therefore, in the case that it is detected in step S210 that at least one sample information exists in the list, the controller may directly proceed to step S230.

Step S240, triggering a microscopy processing thread for executing the sample according to the detection list to obtain fourth information.

Generally, semen samples corresponding to each sample information in the detection list need to be subjected to microscopic examination, so that each sample information can be sequentially selected according to the sample sequence in the table, and a microscopic examination processing thread for executing the samples corresponding to the sample information is triggered; the obtained fourth information comprises the microscopic examination information including adjusting the focus of the objective lens and shooting a sample video or image, or comprises fault information of a certain action error in the microscopic examination processing.

Of course, if there is no sample information in the detection list, the microscopy processing thread cannot be triggered. Therefore, in the case that it is detected in step S210 that at least one sample information exists in the list, the controller may directly proceed to step S240.

And step S250, outputting the first information, the third information and the fourth information.

It should be noted that the first information, the third information, and the fourth information are all test information or failure information, and then the output information may be displayed to a user in a display manner, or may be subjected to subsequent image analysis processing. In addition, since the liquefaction processing thread, the reaction processing thread and the microscopy processing thread are synchronously executed, corresponding information is output only when any one of the liquefaction processing thread, the reaction processing thread and the microscopy processing thread is triggered, for example, if only the reaction processing thread is triggered to be executed, only the third information can be output.

In the embodiment, a reaction processing thread for executing a sample is triggered according to a detection list to obtain third information, and a microscopic examination processing thread for executing the sample is triggered according to the detection list to obtain fourth information, which belong to the detection processing thread for executing the sample according to the detection list to obtain second information; namely: the detection processing threads of the detection list triggering execution samples comprise a reaction processing thread and a microscopic examination processing thread, namely the second information is obtained by using the third information and the fourth information; in some embodiments, the detection processing of the sample according to the trigger of the detection list may also be only a reaction processing thread or a microscopy processing thread, and in this case, the second information is the third information or the fourth information.

In an embodiment, the step S220 is mainly related to a process of triggering a liquefaction processing thread for executing a sample, and then, referring to fig. 4, the step may specifically include steps S221 to S228, which are respectively described below.

Step S211, selecting one piece of sample information from the liquefaction list, and triggering the liquefaction processing thread to enter a liquefaction execution state from the idle state, so as to start liquefaction processing on the sample corresponding to the selected sample information. It is to be understood that when there are a plurality of sample information in the liquefaction list, the controller may select one sample information sequentially or may select one sample information randomly.

Step S222, timing, namely timing the liquefaction process of the sample to obtain first time.

And step S223, a liquefaction detection step, namely detecting whether the first time reaches the second time, and detecting the liquefaction degree when the first time reaches the second time.

In one embodiment, the result of the detection of the liquefaction degree is the viscosity value of the semen sample, and in the liquefaction execution state, a sampling needle is driven to be inserted into the sample container corresponding to the sample information to suck the sample, and the viscosity value of the sample is detected by sensing the internal pressure of the sampling needle.

More specifically, referring to fig. 2, the controller controls the sampling mechanism 11 to perform actions of driving the sampling needle to move in the second direction and the third direction through the sampling travel driving member and reach the sampling position of the sample container a, and driving the sampling needle to be inserted into the sample container a. Then, the sampling driving piece drives the sampling needle to absorb the semen sample in the sample container A, then the pressure sensor arranged at the tail of the sampling needle detects the pressure value in the sampling needle, and the controller calculates the viscosity value of the sample. Then, the controller can judge the liquefaction time needed by the sample according to the viscosity value of the sample.

Of course, if the controller determines that the viscosity value of the sample is greater than the test standard, the sampling driving member may be controlled to drive the sampling member to repeatedly suck and discharge the sample in the sample container, so as to rapidly liquefy the sample in the sample container.

And step S224, performing a classification processing step according to the liquefaction degree detection, outputting a liquefaction result if the liquefaction degree detection result is qualified, determining whether the actual liquefaction time of the current sample reaches the third time if the liquefaction degree detection result is not qualified, stopping liquefaction and outputting prompt information indicating that the current sample is abnormally liquefied if the actual liquefaction time of the current sample reaches the third time, and continuing to perform step S222 (timing step) and step S223 (liquefaction detection step) if the actual liquefaction time of the current sample does not reach the third time.

It should be noted that the second time may be a preset detection time point or a preset detection time period (e.g., a plurality of time nodes such as 5min, 15min, 30min, 1h, etc. from the start of the timing, or a fixed time period such as 10min, 30min, etc. from the start of the timing, and may be repeated one or more times), the third time should be a preset maximum time (e.g., 1h) for allowing liquefaction, and the second time should be less than the third time.

In step S225, it is determined whether or not all operations in the liquefaction execution state are completed, and if so, the process proceeds to step S226, otherwise, the process proceeds to step S227.

The operation in the liquefaction execution state includes at least a sampling needle driving operation, a sample sucking operation, a sampling needle internal pressure detecting operation, and a viscosity value calculating operation, and the process may proceed to step S226 after all of these operations are completed.

In step S226, since all actions in the liquefaction execution state are completed, it indicates that the liquefaction process is completed, and at this time, liquefaction information of the sample may be generated, where the liquefaction information may include a viscosity value of the sample and may also include a liquefaction time of the sample.

In step S227, since the actions in the liquefaction execution state are not all completed, it indicates that a fault occurs in a part of the links, and at this time, first fault information may be generated. For example, if the operation of detecting the internal pressure of the sampling needle cannot be performed, the first fault information that may be generated includes a detection pressure error; and if the semen sample cannot be liquefied, generating prompt information of abnormal liquefaction.

In step S228, first information is obtained using the liquefaction information or the first failure information.

In one embodiment, the above step S224 mainly relates to performing the classification handling process according to the liquefaction degree detection, and then referring to fig. 5, the step specifically includes S2241-S2245, which are respectively described as follows.

Step S2241, comparing the liquefaction degree detection result with a preset threshold value; if the liquefaction degree detection result is less than or equal to the preset threshold value, executing step S2242; if the liquefaction degree detection result is greater than the preset threshold, step S2243 is performed.

Step S2242, a liquefaction result is output.

Step S2243, judging whether the actual liquefaction time of the current sample reaches the third time, if not, executing step S2244; if the actual liquefaction time of the current sample reaches the third time, step S2245 is performed.

Step 2244, extending the second time to obtain a new second time, and comparing the first time with the new second time in the step S223 (liquefaction detection step) to be continuously performed.

And step S2245, outputting prompt information of the current sample liquefaction abnormality.

Therefore, the second time is controlled to be a plurality of time nodes from the timing step, so that the liquefaction degree of the semen sample which is not liquefied can be detected for a plurality of times in the third time, if the second time is set to be 5min, the liquefaction degree is detected when the first time reaches 5min, if the liquefaction degree detection result is unqualified, the second time is prolonged by 10min, so that a new node of the second time is the 15 th min from the timing step, and when the first time reaches 15min, the liquefaction degree is detected; and if the liquefaction degree detection result is still unqualified, continuously prolonging the second time and obtaining new second time until the second time reaches the third time, and performing the last liquefaction degree detection.

In another embodiment, in an embodiment, the step S224 described above mainly involves performing a classification handling process according to the liquefaction degree detection, and then referring to fig. 6, the step specifically includes S2246-S22410, which are respectively described below.

Step S2246, comparing the liquefaction degree detection result with a preset threshold value; if the liquefaction degree detection result is less than or equal to the preset threshold value, executing step S2247; if the liquefaction degree detection result is greater than the preset threshold, step S2248 is performed.

Step S2247, outputs the liquefaction result.

Step 2248, judging whether the actual liquefaction time of the current sample reaches the third time, if not, executing step 2249; if the actual liquefaction time of the current sample reaches the third time, step S22410 is performed.

In step 2249, the first time is cleared, and the timer is started from zero in step S222 (timer step).

And step S22410, outputting prompt information of the current sample liquefaction abnormality.

Therefore, by controlling the second time, the second time is a fixed time period from the timing step, so that the liquefaction degree detection can be performed on the semen sample which is not liquefied for a plurality of times in the third time, namely, the liquefaction degree detection is performed every other second time (such as 5min, 10min and the like), the actual liquefaction time is equivalent to the superposition of a plurality of second times, and the last liquefaction degree detection is performed when the superposed second time reaches the third time.

In this embodiment, the step S230 mainly relates to a process of triggering a reaction processing thread for executing a sample, and then, referring to fig. 7, the step may specifically include steps S231 to S236, which are respectively described as follows.

Step S231 selects a piece of sample information from the detection list, and triggers the reaction processing thread to enter the reaction execution state from the idle state. It is to be understood that when there are a plurality of sample information in the detection list, the controller may select one sample information sequentially or may select one sample information randomly.

Step S232, in the reaction execution state, driving a sampling needle to suck the sample from the sample container corresponding to the sample information and add the sample to a reaction container, capturing a first image of the sample in the reaction container, then injecting a reaction reagent into the reaction container, and capturing a second image of the sample in the reaction container.

In a specific embodiment, referring to fig. 2, the controller controls the sampling mechanism 11 and the reaction processing mechanism 12 to perform some actions, the sampling driving member drives the sampling needle to suck the semen sample in the sample container a, and the sampling walking driving member drives the sampling needle to move in the second direction and the third direction and reach the first sample application position of the reaction container, so as to apply the sucked semen sample to the reaction container. Then, the controller drives the camera to photograph the sample in the reaction container to obtain a first image, then drives the sample adding driving part to inject a reaction reagent (such as a PH reagent) into the reaction container, and drives the camera to photograph the sample in the reaction container again to obtain a second image after the semen sample in the reaction container and the reaction reagent fully react.

It is understood that after the first image and the second image are captured, the controller can analyze the PH of the sample according to the images.

In step S233, it is determined whether all the operations in the reaction execution state are completed, and if so, the process proceeds to step S234, otherwise, the process proceeds to step S235.

It should be noted that the actions in the reaction execution state at least include a sampling needle driving action, a sample adding action, a first photographing action, a reaction reagent adding action, and a second photographing action, and after all the actions are completed, the process may proceed to step S234.

In step S234, since all actions in the reaction execution state are completed, it indicates that the reaction process is completed, and at this time, the reaction information of the sample may be generated, where the reaction information may include the first image and the second image, and may also include the PH value of the sample.

In step S235, since the actions in the reaction execution state are not completed completely, it indicates that a part of the links have a fault, and at this time, second fault information may be generated. For example, if the reagent filling operation cannot be performed, the second fault information that may be generated includes a reagent filling error.

In step S236, third information is obtained by using the response information or the second failure information.

In an embodiment, the step S240 described above mainly relates to a process of triggering a microscopy processing thread for executing a sample, and then, referring to fig. 8, the step may specifically include S241-S246, which are respectively described as follows.

Step S241 selects a piece of sample information from the detection list, and triggers the microscopy processing thread to enter the microscopy execution state from the idle state.

It is to be understood that when there are a plurality of sample information in the microscopy processing list, the controller may select one sample information sequentially or may select one sample information randomly.

Step S242, in the microscopic examination execution state, driving a sampling needle to suck a sample from the sample container corresponding to the sample information and add the sample to a detection card, transferring the detection card to the microscopic examination position and driving a microscope to focus the detection card, and capturing a third image and/or a first video of the sample on the detection card.

In one embodiment, referring to fig. 2, the controller controls the sampling mechanism 11 and the microscopy processing mechanism to perform actions of driving the sampling needle to draw the semen sample in the sample container a by the sampling driving member, and driving the sampling needle to move in the second direction and the third direction to the second loading position of the detection card (e.g., slide) by the sampling traveling driving member, so as to load the drawn semen sample to the detection card. And then, transferring the detection card to a microscopic examination position through a detection card conveying mechanism, and driving a microscope to adjust an eyepiece so as to focus the detection card at the microscopic examination position. And then driving the camera to carry out video shooting and/or image shooting on a focusing area on the detection card from an ocular lens of the microscope, so as to obtain a third image and a first video.

It will be appreciated that after the third image and the first video are captured, the controller can analyze the videos or images to determine the motility status and number of sperm in the semen sample on the test card.

In step S243, it is determined whether all the operations in the microscopic examination execution state are completed, and if so, the process proceeds to step S244, otherwise, the process proceeds to step S245.

It should be noted that the actions in the microscopic examination execution state at least include a sampling needle driving action, a sample adding action, a detection card transferring action, a microscope focusing action, and a photographing action, and the process may proceed to step S244 after all the actions are completed.

In step S244, since all actions in the microscopic examination execution state are completed, it indicates that the microscopic examination process is smooth, and at this time, microscopic examination information of the sample can be generated, where the microscopic examination information may include the third image and the first video, and may also include the moving state and number of sperm in the sample.

In step S245, since the actions in the microscopic examination execution state are not all completed, it indicates that a part of links have a fault, and at this time, third fault information may be generated. For example, if the microscope focusing operation cannot be performed, the third failure information that can be generated includes a microscope focusing error.

In step S246, fourth information is obtained by using the microscopic examination information or the third failure information.

In one embodiment, after any task thread is executed, the corresponding task thread is restored from the execution state to the idle state, thereby facilitating restarting the thread. Specifically, the controller resumes the liquefaction processing thread to enter an idle state after obtaining the first information, and resumes the detection processing thread to enter the idle state after obtaining the second information (for example, resumes the reaction processing thread to enter the idle state after obtaining the third information, and resumes the microscopy processing thread to enter the idle state after obtaining the fourth information).

Of course, when the first information and the second information (i.e., the third information and/or the fourth information) include the failure information, the controller may not immediately restore the corresponding task thread, but may restore the corresponding task thread after the failure is cleared, and continue to enter the execution state, thereby continuing to execute the action where the failure occurs. Currently, in some cases, the corresponding task thread may also be exited after the fault is cleared, leaving the task thread in an idle state.

In one embodiment, for a liquefaction processing thread, a reaction processing thread, or a microscopic processing thread, before triggering execution of any one of the task threads, the idle/execution state of the task thread is checked, and the triggering can be performed only when the task thread is in the idle state.

In one embodiment, when the semen sample needs to be subjected to the reaction processing and the microscopy processing simultaneously, the reaction processing thread and the microscopy processing thread can be triggered to be executed simultaneously, and the process can be described as follows with reference to steps S310-S340 in fig. 9.

In step S310, the states of the reaction processing thread and the specular processing thread are checked.

Step S320, determining whether the reaction processing thread and the microscopy processing thread are idle at the same time, if yes, going to step S330, otherwise, going to step S340.

Step S330, the reaction processing thread is triggered to enter the microscopic examination execution state from the idle state, and simultaneously the microscopic examination processing thread is triggered to enter the microscopic examination execution state from the idle state, and then the two threads are executed respectively. The execution process can refer to steps S231-S236 in fig. 7 and steps S241-S246 in fig. 8, which are not described herein again.

Step S340, if not, the triggering is interrupted until the two threads are idle.

It should be noted that, executing the reaction processing thread and the microscopic examination processing thread simultaneously can reduce the waiting time under some circumstances, because the sampling needle can be used for loading the sample for the reaction container and the detection card at the same time, thereby avoiding the sampling needle from executing the sample sucking action and the sample loading action for many times.

In one embodiment, before acquiring the liquefaction list and the detection list (i.e. before step S210 in fig. 3), a response step is further included, where the response step specifically includes: the controller is used for classifying the sample information of one or more samples to be detected into a liquefaction list or a detection list in response to an input instruction of a user. That is to say, when a certain semen sample is injected, the liquefaction state of the semen sample is artificially identified and judged, and corresponding sample information is directly added into a liquefaction list for the semen sample which does not reach the test standard, so that the semen sample is liquefied firstly.

In one embodiment, after the first information and the second information (i.e. the third information and/or the fourth information) are output (i.e. after step S250 in fig. 3), the method further comprises a displaying step and a fault clearing step: (1) in the display step, the controller displays the first information, the second information (namely, the third information and/or the fourth information) for the user to view; (2) in the fault clearing step, the controller performs fault clearing according to a preset fault handling mechanism, which may be a processing mechanism preset in the controller, such as program self-checking or fault alarm, when the first information and the second information include corresponding fault information. It is understood that after the fault is cleared, the fault can be returned to the error action to continue executing, and the corresponding task thread can be completely exited and wait for the next time to trigger the execution of the thread.

EXAMPLE III

On the basis of the multifunctional sperm quality analyzer provided in the first embodiment, the first embodiment provides a sperm liquefaction method, and the sperm liquefaction detection method is mainly applied to the controller shown in fig. 1, so that only the liquefaction processing mechanism 15 and the sampling mechanism 11 need to be reserved, thereby forming a method specially used for liquefying a semen sample; the specific steps can refer to step 210 and step 220 in example two; the method comprises the following specific steps:

step 410, a step of obtaining a liquefaction list, wherein the liquefaction list comprises one or more pieces of sample information;

step 420, triggering a liquefaction step, namely selecting one piece of sample information from a liquefaction list, and triggering a liquefaction processing thread to enter a liquefaction execution state from an idle state so as to start liquefaction processing on a sample corresponding to the selected sample information;

step 430, timing, namely timing the liquefaction process of the sample to obtain first time;

step 440, a liquefaction detection step, detecting whether the first time reaches a second time, and detecting the liquefaction degree when the first time reaches the second time;

and 450, performing a classification treatment step according to the liquefaction degree detection result, outputting the liquefaction result if the liquefaction degree detection result is qualified, judging whether the actual liquefaction time of the current sample reaches a third time if the liquefaction degree detection result is unqualified, stopping liquefaction if the actual liquefaction time of the current sample reaches the third time, and continuing performing a timing step and a liquefaction detection step if the actual liquefaction time of the current sample does not reach the third time, wherein the second time is less than the third time, and the third time is the preset maximum time for allowing liquefaction.

Example four

On the basis of the multifunctional sperm quality analyzer provided in the first embodiment, a sperm quality detection method is provided in this embodiment, and the sperm quality detection method is mainly applied to the controller shown in fig. 1.

Referring to fig. 10, the detection method in this embodiment includes two parts, one is a UI control part (i.e., human-computer interaction control) and a thread control part, and the former part is formed with display contents on the user interface.

Referring to fig. 10, the controller forms trigger buttons on the user interface that the user utilizes to initiate a sample testing session of the multifunctional sperm quality analyzer. And prompting whether the liquefaction operation is required or not on a user interface for each sample to be injected, if so, adding the sample information of the sample into a liquefaction list, and if not, adding the sample information of the sample into a detection list.

Referring to fig. 10, the controller acquires a liquefaction list and a detection list, triggers a liquefaction processing thread for executing a sample according to the liquefaction list, triggers a reaction processing thread for executing the sample according to the detection list, and triggers a microscopic examination processing thread for executing the sample according to the detection list. The three task threads can be triggered and executed synchronously without the sequence.

In the first case, when one piece of sample information is selected from the liquefaction list and a liquefaction processing thread of a sample is triggered, whether the thread is in an idle state is checked, if so, the liquefaction processing thread is executed, otherwise, a waiting state is carried out until the thread is idle. The process of executing the liquefaction processing thread may refer to steps S221 to S228 in embodiment two.

And in the second case, when one piece of sample information is selected from the detection list and the reaction processing thread of the sample is triggered, whether the thread is in an idle state is checked, if so, the reaction processing thread is executed, otherwise, a waiting state is carried out until the thread is idle. The process of executing the liquefaction processing thread may refer to steps S231 to S236 in embodiment two.

And in the third case, when one piece of sample information is selected from the detection list and the microscopic examination processing thread of the sample is triggered, whether the thread is in an idle state is checked, if so, the microscopic examination processing thread is executed, otherwise, a waiting state is carried out until the thread is idle. The process of executing the mirroring processing thread may refer to steps S241 to S246 in embodiment two.

Referring to fig. 10, after each task thread is finished, test information and fault information of the task thread are obtained, for example, after a liquefaction processing thread for executing a sample is triggered, first information is obtained, after a reaction processing thread for executing the sample is triggered, second information is obtained, after a microscopic examination processing thread for executing the sample is triggered, third information is obtained, and then the controller may output the information.

Referring to fig. 10, when the test information and the failure information are output to the user interface, the information is displayed on the user interface for the user to view in time.

EXAMPLE five

On the basis of the detection method provided in the second embodiment or the third embodiment, the present embodiment provides a multifunctional sperm quality analyzer.

Referring to fig. 11, the multifunctional sperm cell mass analyzer 4 includes a memory 41 and a processor 42. The memory 41 is used to store a program, and the program may be a program code corresponding to the detection method in the second embodiment, or a program code corresponding to the detection method in the third embodiment.

The processor 42 is connected to the memory 41 for executing the program stored in the memory 41 to implement the detection method. The functions implemented by the processor 42 can refer to the controller in the second embodiment or the third embodiment, and will not be described in detail here.

Those skilled in the art will appreciate that all or part of the functions of the various methods in the above embodiments may be implemented by hardware, or may be implemented by computer programs. When all or part of the functions of the above embodiments are implemented by a computer program, the program may be stored in a computer-readable storage medium, and the storage medium may include: a read only memory, a random access memory, a magnetic disk, an optical disk, a hard disk, etc., and the program is executed by a computer to realize the above functions. For example, the program may be stored in a memory of the device, and when the program in the memory is executed by the processor, all or part of the functions described above can be implemented. In addition, when all or part of the functions in the above embodiments are implemented by a computer program, the program may be stored in a storage medium such as a server, another computer, a magnetic disk, an optical disk, a flash disk, or a removable hard disk, and may be downloaded or copied to a memory of a local device, or may be version-updated in a system of the local device, and when the program in the memory is executed by a processor, all or part of the functions in the above embodiments may be implemented.

The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. Numerous simple deductions, modifications or substitutions may also be made by those skilled in the art in light of the present teachings.

Claims

1. A method of liquefying sperm, comprising:

a step of triggering liquefaction, namely selecting one piece of sample information from the liquefaction list, and triggering the liquefaction processing thread to enter a liquefaction execution state from an idle state so as to start liquefaction processing on a sample corresponding to the selected sample information;

2. A method of sperm quality detection comprising:

a step of triggering liquefaction, namely selecting one piece of sample information from the liquefaction list, and triggering an idle state in the liquefaction processing thread to enter a liquefaction execution state so as to start liquefaction processing on a sample corresponding to the selected sample information;

3. The method of claim 1 or 2, wherein the step of categorizing comprises:

4. The method of claim 1 or 2, wherein the step of categorizing comprises:

5. The method of claim 1 or 2, further comprising, after stopping liquefaction: and outputting prompt information indicating that the current sample is abnormal in liquefaction.

6. The method of claim 1 or 2, wherein the detection of the degree of liquefaction is a viscosity value of the semen sample.

7. The method of claim 6, wherein performing liquefaction level detection comprises:

8. The method of claim 2, wherein triggering a detection processing thread to execute samples according to the detection list to obtain second information comprises:

9. A multifunctional sperm quality analyzer is characterized by comprising a liquefaction processing mechanism, a sampling mechanism, a detection processing mechanism and a controller;

the controller is in signal connection with the liquefaction processing mechanism, the sampling mechanism and the detection processing mechanism for sequential control of the liquefaction processing mechanism, the sampling mechanism and the detection mechanism by the method of any one of claims 1-8.

10. A computer-readable storage medium, characterized by comprising a program executable by a processor to implement the method of any one of claims 1-8.