CN110618286A - Sperm liquefaction degree analysis device and analysis method thereof - Google Patents
Sperm liquefaction degree analysis device and analysis method thereof Download PDFInfo
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- CN110618286A CN110618286A CN201910963008.0A CN201910963008A CN110618286A CN 110618286 A CN110618286 A CN 110618286A CN 201910963008 A CN201910963008 A CN 201910963008A CN 110618286 A CN110618286 A CN 110618286A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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Abstract
The invention provides a sperm liquefaction degree analysis device which comprises a sample adding needle, a pump, a controller and an air pressure acquisition device, wherein the controller is electrically connected with the pump and the air pressure acquisition device, the sample adding needle is hermetically connected with an inlet of the pump through a pipeline, and the air pressure acquisition device is arranged between the sample adding needle and the pump. The invention also provides a sperm liquefaction degree analysis method, which comprises the sperm liquefaction degree analysis device and comprises the following steps: the sperm liquefaction degree analysis device is used for carrying out suction and spitting actions on the semen before liquefaction and after liquefaction so as to obtain air pressure change data in the pipeline in the suction and spitting action process. Compared with the prior art, the semen sample is normal or not after the air pressure data of the air pressure change in the pipeline in the sucking and spitting process is obtained by the air pressure collecting device, so that the error of detection and analysis by manual visual inspection can be avoided, and the analysis precision is improved; because detect through the machine, removed artifical manual detection from, use manpower sparingly.
Description
Technical Field
The invention relates to a detection technology in the medical field, in particular to a sperm liquefaction degree analysis device and an analysis method.
Background
The semen becomes a gel after it has been expelled from the body, and at room temperature within minutes, the semen usually begins to liquefy (thin), where uneven clumping can be seen in the liquid. As liquefaction continues over time, the semen becomes a homogeneous watery mass and eventually only very small clots are seen, generally, complete liquefaction usually occurs within 15 minutes at room temperature, rarely exceeding 60 minutes, and unliquefied semen can interfere with subsequent analysis of the semen.
At present, the analysis of the liquefaction degree of the semen is a manual analysis method: namely, a plastic suction pipe with the caliber of about 1.5mm is used for slowly sucking the semen, the length of mucus thread formed by the semen under the action of the gravity center is observed, the semen drops continuously fall normally, and the length of the mucus thread exceeds 2cm abnormally; or inserting the glass rod into semen for observation, and lifting to observe the length of mucus thread formed, wherein the length exceeding 2cm is abnormal. By adopting the method, not only is the operation of great manpower and material resources needed, but also certain influence is caused to the analysis result due to the individual difference of the detection personnel, so that the risk of judgment errors is increased. How to analyze the semen viscosity by technical means is a topic worthy of research.
Disclosure of Invention
The invention aims to provide a sperm liquefaction degree analysis device and an analysis method thereof, and aims to solve the technical problems of replacing manual detection, saving labor and improving analysis precision.
In order to solve the problems, the invention adopts the following technical scheme: the utility model provides a sperm liquefaction degree analytical equipment, includes application of sample needle, pump, controller and atmospheric pressure collection system, the controller is connected with pump and atmospheric pressure collection system electricity, the application of sample needle is through pipeline and the import sealing connection of pump to make the pump absorb to the semen sample through the application of sample, atmospheric pressure collection system sets up between application of sample needle and pump, in order to realize when the pump is inhaled the semen sample and is told, atmospheric pressure collection system acquires the change data of atmospheric pressure in the pipeline, the controller will change data and export, the change data of pipeline internal pressure are judged when the inspector is inhaled and is told through artificial mode, thereby whether it is normal to reachd the semen sample.
And the controller is connected and communicated with the computer through a serial port so as to realize the collection of the change data of the air pressure in the pipeline acquired by the air pressure acquisition device, convert the change data into air pressure value data and send the air pressure value data to the computer for output.
Further, the pump is a plunger pump.
Furthermore, the sample adding needle is detachably connected with one end of the pipeline in a sealing manner, so that the sample adding needle is replaced.
Further, the air pressure acquisition device is an air pressure sensor.
The invention also discloses a sperm liquefaction degree analysis method, which comprises the sperm liquefaction degree analysis device, and the sperm liquefaction degree analysis method comprises the following steps: the semen before liquefaction and after liquefaction is sucked and spitted by the sperm liquefaction degree analysis device to obtain the air pressure change data in the pipeline in the sucking and spitting action process, and the change data of the air pressure in the pipeline is judged by a detection person in a manual mode when the semen is sucked and spitted, so that whether the semen sample is normal or not is obtained.
Furthermore, the sperm liquefaction degree analysis device also comprises a computer, and the controller is connected and communicated with the computer through a serial port; the sperm liquefaction degree analysis device is used for absorbing and spitting the semen before liquefaction and after liquefaction so as to obtain the air pressure change data in the pipeline in the absorbing and spitting action process:
before the semen is liquefied, a controller controls a pump to suck and spit the semen, meanwhile, air pressure change data in a pipeline in the process of the sucking and spitting actions are obtained through an air pressure collecting device, and the controller converts the air pressure change data to obtain first air pressure value data and then sends the first air pressure value data to a computer;
step two, at room temperature, after the semen is liquefied, the controller controls the pump to suck and spit the semen again, meanwhile, the air pressure change data in the pipeline in the sucking and spitting action process is obtained through the air pressure collecting device 4, and the controller converts the air pressure change data to obtain second air pressure value data and then sends the second air pressure value data to the computer;
and step three, outputting the first air pressure value data and the second air pressure value data by the computer.
Further, the step one of acquiring the air pressure change data in the pipeline in the suction and discharge action process through the air pressure acquisition device is to start acquiring the air pressure change data in the pipeline in the suction and discharge action process through the air pressure acquisition device after 2 seconds from the start of the suction action by the controller.
Further, the step two of acquiring the air pressure change data in the pipeline in the suction and discharge action process through the air pressure acquisition device specifically means that the controller starts to acquire the air pressure change data in the pipeline in the suction and discharge action process through the air pressure acquisition device 2 seconds after the suction action starts.
Further, the output in step three is generated into a comparison graph.
Compared with the prior art, the invention is provided with the pump, the air pressure acquisition device and the controller, the pump is used for repeatedly sucking and spitting non-liquefied semen and liquefied semen, the controller utilizes the air pressure acquisition device to obtain air pressure value data of air pressure change in the pipeline in the sucking and spitting process, and a detector judges the air pressure value data in a manual or mechanical mode, so that whether a semen sample is normal or not is obtained, the air pressure acquisition device is used for obtaining the air pressure change in the pipeline, the error of detection and analysis by manual visual inspection can be avoided, and the analysis precision is improved; because detect through the machine, removed artifical manual detection from, use manpower sparingly.
Drawings
FIG. 1 is a schematic view showing the configuration of a sperm liquefaction degree analysis apparatus according to the present invention.
FIG. 2 is a flowchart of a method for analyzing the degree of liquefaction of sperm according to the present invention.
FIG. 3 is a comparative illustration of the degree of liquefaction of semen obtained by the analysis method of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
As shown in fig. 1, the invention discloses a sperm liquefaction degree analysis device, which comprises a sample adding needle 1, a pump 2, a controller 3 and an air pressure acquisition device 4, wherein the controller 3 is electrically connected with the pump 2 and the air pressure acquisition device 4, and the controller 3 controls the pump 2 and the air pressure acquisition device 4; sample adding needle 1 is through the import sealing connection of pipeline 6 and pump 2, so that pump 2 absorbs the semen sample through sample adding needle 1, sample adding needle 1 can adopt prior art's sample adding needle, certainly also can adopt disposable sample adding needle, atmospheric pressure collection device 4 sets up between sample adding needle 1 and pump 2, when realizing that pump 2 inhales the semen sample and spits, atmospheric pressure collection device 4 sends controller 3 after acquireing the change data of 6 internal gas pressures of pipeline, controller 3 exports the change data, the change data of pipeline 6 internal gas pressure when detecting personnel's accessible is artifical or the mode of machine is judged inhaling when spitting, thereby whether reachs the semen sample normal.
The controller 3 may output the change data to an external display for display or print via a printer, using conventional techniques.
By adopting the mode, the problem of low accuracy of results caused by manual visual inspection can be avoided, and the air pressure acquisition device 4 is adopted to acquire the change of the air pressure in the pipeline 6, so that the change of the air pressure in the pipeline 6 during suction and spitting can be judged manually or mechanically.
As shown in fig. 1, the air pressure collecting device 4 further includes a computer 5, the controller 3 is connected to and communicates with the computer 5 through a serial port, so that the controller 3 collects the change data of the air pressure in the pipeline, which is acquired by the air pressure collecting device 4, converts the change data into air pressure value data, and sends the air pressure value data to the computer 5, and the computer 5 outputs and/or stores the air pressure value data in a memory of the computer 5.
The computer 5 may output the air pressure value data by generating a comparison graph, which is a graph, from the air pressure value data and outputting the comparison graph by a printer or a display device.
In the invention, the controller 3 is electrically connected with the air pressure acquisition device 4 through the SPI interface so as to acquire the change data of the air pressure through the air pressure acquisition device 4.
In order to ensure the sucking and spitting effect, the pump 2 can adopt a plunger pump; the air pressure acquisition device 4 adopts an air pressure sensor.
The controller 3 respectively performs suction and spitting actions on the semen before liquefaction and the semen after liquefaction through the pump, and obtains air pressure change data in the pipeline in the suction and spitting processes of the semen before liquefaction and the semen after liquefaction through the air pressure collecting device 4 to obtain first air pressure value data of the semen before liquefaction and second air pressure value data of the semen after liquefaction and send the first air pressure value data and the second air pressure value data to the computer 5.
The computer 5 is a computer provided with a Windows operating system, and data acquisition software (such as a serial assistant) for collecting signal data is also arranged in the computer 5 so as to count the air pressure value data sent by the controller 3 and generate a statistical list file; the statistical list file is a text file in a TXT format; the data acquisition software is the prior art and is not specifically limited herein; the statistical list file comprises time information and a pressure value corresponding to the time information.
The computer 5 generates a comparison graph according to the air pressure value data and outputs the following specific steps: the computer 5 respectively generates corresponding statistical list files according to the first air pressure value data and the second air pressure value data, then the two statistical list files are simultaneously input into Excel software to generate a comparison graph and then output, and the output can be displayed through a display of the computer 5 or printed and output through a printer; the comparison graph may be a graph, preferably a graph.
The Excel software may be Excel software in WPS office software of the seikari kingdom office software ltd or Excel software in Microsoft office software of Microsoft corporation.
The method for generating the comparison graph by inputting the statistical list files into the Excel software specifically comprises the steps of inputting two statistical list files into the Excel software respectively in an input mode, generating the comparison graph by selecting data in two groups of input statistical list files and then inserting the data into the graph, and then storing and outputting the comparison graph.
As shown in fig. 1, the sample adding needle 1 is detachably connected with one end of the pipeline 6 in a sealing way, so that the sample adding needle 1 can be replaced; specifically, a rotatable nut 11 is arranged on the sample adding needle 1, an external thread is arranged on the outer wall of the pipeline at one end of the pipeline 6 opposite to the sample adding needle 1, so that the sample adding needle 1 is in threaded connection with the external thread on the pipeline 6 through the nut 11, and a sealing ring is arranged in the nut 11 to realize sealing connection; of course, luer connectors can be used to connect the sample injection needle 1 with the conduit 6.
As shown in fig. 2, the present invention also discloses a method for analyzing the liquefaction degree of sperm, comprising the above-mentioned apparatus for analyzing the liquefaction degree of sperm, the method comprising: the sperm liquefaction degree analysis device is used for carrying out suction and spitting actions on the semen before liquefaction and after liquefaction so as to obtain air pressure change data in the pipeline in the suction and spitting action process.
Specifically, the sperm liquefaction degree analysis device further comprises a computer 5, and the controller 3 is connected and communicated with the computer 5 through a serial port; the method comprises the following steps:
step one S01, before the semen is liquefied, the controller 3 controls the pump 2 to perform the sucking and spitting actions on the semen, meanwhile, the air pressure change data in the pipeline 6 in the sucking and spitting action process is obtained through the air pressure collecting device 4, and the controller 3 converts the air pressure change data to obtain first air pressure value data and then sends the first air pressure value data to the computer 5; specifically, the controller 3 starts to acquire the change data of the air pressure in the pipe 6 during the suction and discharge actions by the air pressure acquisition device 4 2 seconds after the start of the suction actions. In general, the total time required for suction and discharge is 15 seconds.
Step two S02, after the semen is liquefied, the controller 3 controls the pump 2 to suck and spit the semen again, meanwhile, the air pressure change data in the pipeline 6 in the sucking and spitting action process is obtained through the air pressure collecting device 4, and the controller 3 converts the air pressure change data to obtain second air pressure value data and then sends the second air pressure value data to the computer 5; specifically, the controller 3 starts to acquire the air pressure change data in the pipe 6 during the suction and discharge actions through the air pressure acquisition device 4 2 seconds after the start of the suction actions. Generally, the time required for the whole sucking and spitting is 15 seconds; the semen liquefaction time is 15-60 minutes.
Step three S03, the computer 5 outputs the first air pressure value data and the second air pressure value data, and the output is output after generating the comparison graph; specifically, the computer 5 firstly carries out statistics on first air pressure value data through data acquisition software to generate a corresponding first statistic list file; then after receiving the second air pressure value data, counting the second air pressure value data through data acquisition software to generate a corresponding second statistical list file; then inputting the first statistical list file and the second statistical list file into Excel software to generate a comparison graph and then outputting the comparison graph; the comparison is graphically represented as a graph (as shown in fig. 3).
Fig. 3 shows that the same semen sample is sampled twice before and after liquefaction to obtain four sets of air pressure value data, which can further improve the sample accuracy.
As shown in figure 3, when a comparative illustration is obtained, it can be seen that before the semen 1 and the semen 2 are liquefied, when the pump 2 is performing the action of sucking the semen, the trough of the pressure curve is at a position of-4 or below, and when the pump 2 is performing the action of spitting the semen, the peak of the pressure curve is at a position of more than 4 and less than 6; after the semen 1 and the semen 2 are liquefied, when the pump 2 performs an action of sucking the semen, the pressure curve is a position where negative pressure with small fluctuation is generated, the trough is close to zero, and when the pump 2 spits the semen, the peak of the pressure curve tends to 0; the peak and the trough before and after liquefaction in this comparison graphic representation of testing personnel accessible contrast to judge whether the semen liquefies completely, thereby distinguish whether the semen liquefies, realize the analysis of semen liquefaction degree.
Compared with the manual analysis of the semen liquefaction degree, the method avoids manual intervention. The semen liquefaction degree is quantified, direct contact of a doctor on a semen sample can be reduced under the condition that the analysis accuracy is not reduced, and the conventional work environment of the semen is optimized.
Claims (10)
1. A sperm liquefaction degree analysis device, characterized in that: including application of sample needle (1), pump (2), controller (3) and atmospheric pressure collection system (4), controller (3) are connected with pump (2) and atmospheric pressure collection system (4) electricity, application of sample needle (1) is through the import sealing connection of pipeline (6) and pump (2) to make pump (2) absorb the semen sample through application of sample needle (1), atmospheric pressure collection system (4) set up between application of sample needle (1) and pump (2), when realizing inhaling the semen sample in pump (2), atmospheric pressure collection system (4) acquire the change data of pipeline (6) internal gas pressure, controller (3) will change data and export, the change data of pipeline 6 internal gas pressure when testing personnel are inhaling and spitting through artificial mode is judged, thereby whether reacing the semen sample is normal.
2. The sperm liquefaction degree analysis device according to claim 1, wherein: the pipeline air pressure monitoring device is characterized by further comprising a computer (5), the controller (3) is connected with the computer (5) through a serial port for communication, so that the change data of the air pressure in the pipeline acquired by the air pressure acquisition device (4) can be acquired and converted into air pressure value data, and then the air pressure value data is sent to the computer (5) for output.
3. The sperm liquefaction degree analysis device according to claim 1, wherein: the pump (2) is a plunger pump.
4. The sperm liquefaction degree analysis device according to claim 1, wherein: sample adding needle (1) detachably and pipeline (6) one end sealing connection to the change to realize sample adding needle (1).
5. The sperm liquefaction degree analysis device according to claim 2, wherein: the air pressure acquisition device (4) is an air pressure sensor.
6. A method for analyzing the liquefaction degree of sperm, which is characterized in that: a sperm liquefaction degree analysis apparatus comprising the sperm liquefaction degree analysis apparatus according to claim 1, the sperm liquefaction degree analysis method comprising: the semen before liquefaction and after liquefaction is sucked and spitted by the sperm liquefaction degree analysis device to obtain the air pressure change data in the pipeline in the sucking and spitting action process, and the change data of the air pressure in the pipeline is judged by a detection person in a manual mode when the semen is sucked and spitted, so that whether the semen sample is normal or not is obtained.
7. The method for analyzing the degree of liquefaction of sperm according to claim 6, wherein: the sperm liquefaction degree analysis device also comprises a computer (5), and the controller (3) is connected and communicated with the computer (5) through a serial port; the sperm liquefaction degree analysis device is used for absorbing and spitting the semen before liquefaction and after liquefaction so as to obtain the air pressure change data in the pipeline in the absorbing and spitting action process:
before the semen is liquefied, a controller (3) controls a pump (2) to suck and spit the semen, meanwhile, air pressure change data in a pipeline (6) in the sucking and spitting action process are obtained through an air pressure collecting device (4), and the controller (3) converts the air pressure change data to obtain first air pressure value data and then sends the first air pressure value data to a computer (5);
step two, after the semen is liquefied at room temperature, the controller (3) controls the pump (2) to suck and spit the semen again, meanwhile, the air pressure change data in the pipeline (6) in the sucking and spitting action process are obtained through the air pressure collecting device (4), and the controller (3) converts the air pressure change data to obtain second air pressure value data and sends the second air pressure value data to the computer (5);
and step three, outputting the first air pressure value data and the second air pressure value data by the computer (5).
8. The method for analyzing the degree of liquefaction of sperm according to claim 7, wherein: in the first step, the air pressure change data in the pipeline (6) in the suction and discharge action process is acquired through the air pressure acquisition device (4), specifically, the controller (3) starts to acquire the air pressure change data in the pipeline (6) in the suction and discharge action process through the air pressure acquisition device (4) 2 seconds after the suction action starts.
9. The method for analyzing the degree of liquefaction of sperm according to claim 7, wherein: and in the second step, the air pressure change data in the pipeline (6) in the suction and discharge action process is acquired through the air pressure acquisition device (4), specifically, the controller (3) starts to acquire the air pressure change data in the pipeline (6) in the suction and discharge action process through the air pressure acquisition device (4) 2 seconds after the suction action starts.
10. The method for analyzing the degree of liquefaction of sperm according to claim 7, wherein: the output in step three is a comparative graphical representation.
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| CN201910963008.0A CN110618286A (en) | 2019-10-11 | 2019-10-11 | Sperm liquefaction degree analysis device and analysis method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113984585A (en) * | 2021-11-02 | 2022-01-28 | 吉林市国科医工科技发展有限公司 | Sperm liquefaction degree analysis method based on image |
| CN114002103A (en) * | 2021-11-04 | 2022-02-01 | 吉林市国科医工科技发展有限公司 | Method for analyzing liquefaction degree of semen |
| CN114441249A (en) * | 2020-10-30 | 2022-05-06 | 深圳市瑞图生物技术有限公司 | Sperm liquefying method, sperm quality detecting method, sperm quality analyzer and storage medium |
| CN114441390A (en) * | 2020-10-30 | 2022-05-06 | 深圳市瑞图生物技术有限公司 | Concurrent time sequence control method, sperm quality analyzer and storage medium |
-
2019
- 2019-10-11 CN CN201910963008.0A patent/CN110618286A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114441249A (en) * | 2020-10-30 | 2022-05-06 | 深圳市瑞图生物技术有限公司 | Sperm liquefying method, sperm quality detecting method, sperm quality analyzer and storage medium |
| CN114441390A (en) * | 2020-10-30 | 2022-05-06 | 深圳市瑞图生物技术有限公司 | Concurrent time sequence control method, sperm quality analyzer and storage medium |
| CN113984585A (en) * | 2021-11-02 | 2022-01-28 | 吉林市国科医工科技发展有限公司 | Sperm liquefaction degree analysis method based on image |
| CN114002103A (en) * | 2021-11-04 | 2022-02-01 | 吉林市国科医工科技发展有限公司 | Method for analyzing liquefaction degree of semen |
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