WO2021254289A1

WO2021254289A1 - Extraction device for fat tissue extract

Info

Publication number: WO2021254289A1
Application number: PCT/CN2021/099864
Authority: WO
Inventors: 王斌; 张文杰
Original assignee: 上海萨美细胞技术有限公司
Priority date: 2020-06-19
Filing date: 2021-06-11
Publication date: 2021-12-23
Also published as: CN213760828U

Abstract

An extraction device used for fat tissue extract, the device comprising a fat separation module, a fat emulsification module, an emulsified fat separation module, a filtering module and a collection module. The extraction device used for fat tissue extract is able to easily and conveniently perform extraction on fat tissue to obtain an extract enriched with growth factors.

Description

Extraction device for adipose tissue extracting liquid

Technical field

The invention and the field of biopharmaceutical equipment, in particular, relates to an extraction device for adipose tissue extracting liquid.

Background technique

Nano fat is a product obtained by mechanically cutting adipose tissue and chylolysis, which contains lipid droplets, stromal cells and a variety of growth factors, and is used in soft tissue filling. The study found that centrifugation of nano fat can be separated to obtain an extract rich in growth factors.

In the prior art, there are many devices involved in the process of extracting the growth factors rich in adipose tissue, the operation is cumbersome, and these numerous devices are difficult to obtain in the same laboratory at the same time. In the process of extracting and obtaining an extract containing growth factors, It takes a long time to prepare and reduces work efficiency.

Therefore, there is a need in the art to develop a device that can be simply and conveniently used for extracting and obtaining an extract rich in growth factors from adipose tissue.

Summary of the invention

The purpose of the present invention is to provide a simple and convenient device for extracting and obtaining an extract rich in growth factors from adipose tissue.

In a first aspect of the present invention, there is provided an extraction device for adipose tissue extract, the device includes a fat separation module, a fat emulsification module, an emulsified fat stratification module, a filtration module, and a collection module;

The fat separation module includes a first centrifuge 1 and a first syringe barrel 2, the first centrifuge includes a first centrifuge cavity 3, and the first syringe barrel can be centrifuged in the first centrifuge cavity, so The front end of the first syringe barrel is provided with a first pipe joint 4, and the first pipe joint is detachably connected with the plug 5;

The fat emulsification module includes an emulsifier 6, a second syringe barrel 7 and a three-way connector 8. The emulsifier includes an emulsification cavity, the emulsification cavity is connected to the first inlet pipe 9, and the first The front end of the second syringe barrel is provided with a second pipe joint 10. The three-way connector includes a first port 11, a second port 12, and a third port 13. The first syringe barrel can pass through the A pipe joint can be detachably connected with the first port, the second syringe barrel can be detachably connected with the second port through a second pipe joint, and the emulsification cavity can be detachably connected with the first port. The liquid pipe is connected with the third port;

The emulsified fat stratification module includes a second centrifuge 14, the second centrifuge includes a second centrifuge cavity 15, and the first syringe barrel can be centrifuged in the second centrifuge cavity;

The filter module includes a filter 16, and the liquid in the first syringe barrel can be filtered in the filter;

The collection module includes a collector 17 which collects the adipose tissue extract filtered by the filter.

In another preferred embodiment, the adipose tissue extract contains cytokines.

In another preferred embodiment, the fat passes through the fat separation module, the fat emulsification module, the emulsified fat stratification module, the filtration module and the collection module in sequence to obtain the adipose tissue extract.

In another preferred embodiment, in the fat separation module, the first centrifuge can centrifuge the fat in the first syringe barrel to separate purified fat, and the purified fat is located in the first syringe barrel;

In the fat emulsification module, the first syringe barrel containing purified fat obtained by centrifugation of the fat separation module is connected to the first port through a first pipe joint, and the second syringe barrel is connected to the first port through a second pipe joint. The two ports are connected, the emulsification cavity is connected to the third port through the first liquid inlet pipe, and the first syringe barrel and the second syringe barrel are repeatedly beaten to make the purified fat emulsify in the emulsification cavity. Obtain nano fat, where the nano fat is located in the first syringe barrel;

In the emulsified fat stratification module, the fat emulsification module emulsifies the first syringe barrel containing nano fat to be centrifuged in the second centrifugal cavity of the second centrifuge to separate and obtain the preliminary product of the lower adipose tissue extract. The initial product of adipose tissue extract is located in the first syringe barrel;

In the filtering module, the emulsified fat stratification module obtains a first syringe barrel containing a primary adipose tissue extract and is connected to a filter, and the primary adipose tissue extract in the first syringe barrel is filtered and purified by the filter to obtain adipose tissue Extract

In the collecting module, the collector collects the nano fat obtained by the emulsified fat layering module.

In another preferred embodiment, the fat is fat obtained by sucking from fat tissues of animals (including humans).

In another preferred embodiment, the device further includes a fat cleaning module, and the fat cleaning module is used to clean fat.

In another preferred embodiment, the fat cleaning module includes a first syringe barrel, the fat and the cleaning fluid are mixed in the first syringe barrel and then statically layered, the bottom water phase is removed, and then the cleaning fluid is added to the first syringe barrel. After mixing, it is statically stratified and washed repeatedly to obtain washed fat, and the washed fat is located in the first syringe barrel.

In another preferred embodiment, the fat enters the fat separation module after being cleaned by the fat cleaning module.

In another preferred embodiment, the cleaning solution is physiological saline.

In another preferred embodiment, the washing solution is PBS buffer.

In another preferred embodiment, the device further includes a storage module, and the storage module is used to store the adipose tissue extract collected in the collector.

In another preferred embodiment, the storage module is a refrigerator.

In another preferred embodiment, the plug can block the first pipe joint.

In another preferred embodiment, the plug can block the second pipe joint.

In another preferred example, the first syringe barrel and the first syringe barrel include a push rod 18 and a piston 19, the piston is tightly connected with the inner wall of the syringe barrel, and the push rod is connected to the piston, The push rod can push the piston to freely slide back and forth along the inner wall of the syringe barrel.

In another preferred embodiment, the first syringe barrel and the second syringe barrel are the same.

In another preferred embodiment, the filter is a microporous membrane filter.

In another preferred example, the microporous membrane filter includes a liquid inlet 20, a liquid outlet 21, and a microporous membrane. The liquid flows in from the liquid inlet, and is filtered through the microporous membrane from the liquid outlet. Outflow.

In another preferred embodiment, the first syringe barrel is detachably connected to the liquid inlet of the microporous membrane filter through a first pipe joint.

In another preferred example, the pore size of the microporous filter membrane is 0.1-0.8 μm.

In another preferred example, the pore size of the microporous filter membrane is 0.15-0.3 μm, preferably 0.22 μm.

In another preferred embodiment, the emulsifier is a homogeneous emulsifier.

In another preferred embodiment, the homogenizing emulsifier is a mechanical homogenizing emulsifier.

In another preferred embodiment, the liquid is emulsified in the emulsification cavity.

In another preferred embodiment, the inner walls of the first port, the second port and the third port are provided with spiral rings, and the first pipe joint, the second pipe joint and the first liquid inlet pipe are connected with each other. The connection of the first port, the second port and the third port is provided with threads, and the threads can be rotated and fixed on the spiral ring.

In another preferred embodiment, the collector is connected to the filtrate outlet of the filter through a conveying pipe.

In another preferred embodiment, the delivery pipeline is provided with a valve.

In another preferred embodiment, the collector is a collecting tube.

In another preferred embodiment, the connection is a fixed connection or a detachable connection.

In another preferred embodiment, the connection is communication.

It should be understood that within the scope of the present invention, the above-mentioned technical features of the present invention and the technical features specifically described in the following (such as the embodiments) can be combined with each other to form a new or preferred technical solution. Due to space limitations, I will not repeat them one by one here.

Description of the drawings

The attached drawings are only for illustrative purposes and cannot be understood as a limitation of this patent; in order to better illustrate this embodiment, some parts of the attached drawings may be omitted, enlarged or reduced, and do not represent the size of the actual product; For the personnel, it is understandable that some well-known structures and their descriptions in the drawings may be omitted; the same or similar reference numerals correspond to the same or similar components; the terms describing the positional relationship in the drawings are only for exemplary description and cannot be understood. It is a limitation of this patent.

Fig. 1 is a schematic structural diagram of an extraction device for adipose tissue extract in a preferred embodiment of the present invention.

1 is the first centrifuge, 2 is the first syringe barrel, 3 is the first centrifugal chamber, 4 is the first pipe joint, 5 is the plug, 6 is the emulsifier, 7 is the second syringe barrel, and 8 is the three-way connection Device, 9 is the first inlet pipe, 10 is the second pipe joint, 11 is the first port, 12 is the second port, 13 is the third port, 14 is the second centrifuge, 15 is the second centrifuge The cavity, 16 is a filter, 17 is a collector, 18 is a push rod, 19 is a piston, 20 is a liquid inlet and 21 is a liquid outlet.

detailed description

After extensive and in-depth research, the inventor has developed an extraction device for adipose tissue extract. In the extraction device for adipose tissue extract of the present invention, the fat can pass through the fat separation module, the fat emulsification module, the emulsified fat stratification module, the filtration module and the collection module in order to obtain the adipose tissue extraction rich in growth factors Liquid, the device of the present invention is simple, convenient and easy to industrialize production. On this basis, the inventor completed the present invention.

the term

Unless otherwise defined, all technical and scientific terms used herein have the same meanings as commonly understood by those of ordinary skill in the art to which the present invention belongs.

As used herein, the terms "including," "including," and "containing" are used interchangeably, and include not only open-ended definitions, but also semi-closed and closed-ended definitions. In other words, the term includes "consisting of" and "consisting essentially of".

As used herein, the terms "first", "second", "third", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Thus, the features defined as “first”, “second”, “third”, etc. may explicitly or implicitly include one or more of these features.

As used herein, the terms "connected" or "connected" can be used interchangeably, and should be understood in a broad sense. It can be a fixed connection or connection, a detachable connection or connection, or an integral connection or connection.

For ease of description, the present invention will be further described below in conjunction with FIG. 1. It should be understood in the present invention that the accompanying drawings do not limit the scope of the present invention.

Typically, the present invention provides an extraction device for adipose tissue extract, the device includes a fat separation module, a fat emulsification module, an emulsified fat stratification module, a filtration module and a collection module;

The collection module includes a collector 17, and the collector collects the fat tissue extract filtered by the filter.

The extraction device for adipose tissue extract of the present invention can be simple and convenient to extract from adipose tissue to obtain an extract rich in growth factors. Typically, its main working principle includes the steps:

(1) Purified fat is obtained after the sucked fat is separated by the fat separation module. The specific steps are: add the sucked fat to the first syringe barrel, block the first pipe joint with a plug, and then plug the first syringe barrel Put it into the first centrifugal cavity of the first centrifuge for centrifugation. The fat in the first syringe barrel is separated, the lower water phase (including blood water), and the upper layer is purified fat. After centrifugation, open the plug and put it in the first syringe barrel. After the lower water phase (including blood water) flows out through the first pipe joint and is removed, the first pipe joint is blocked again with a plug to obtain a first syringe barrel containing purified fat;

(2) The purified fat is emulsified in the fat emulsification module to obtain nanofat. The specific steps are: open the plug on the first pipe joint of the first syringe barrel containing purified fat, and connect the first pipe joint of the first syringe barrel The first port of the three-way connector connects the second syringe barrel with the second port through the second pipe joint, and connects the emulsification cavity to the third port through the first inlet pipe, and connects the emulsification cavity to the third port through the first syringe The barrel and the second syringe barrel repeatedly fight (for example, the plunger is pushed by a push rod to slide freely back and forth along the inner wall of the syringe barrel, and the purified fat will continue to enter and exit the emulsification cavity), so that the purified fat is emulsified in the emulsification cavity , To obtain nano fat, and suck the nano fat into the first syringe barrel through suction of the first syringe barrel;

(3) The nano fat is stratified in the emulsified fat stratification module to separate the primary adipose tissue extract. The specific steps are: the first syringe barrel containing the nano fat is carried out in the second centrifugal cavity of the second centrifuge Centrifugation, stratification, the upper layer is oil droplets, the lower layer is the primary adipose tissue extract (aqueous phase), the primary adipose tissue extract of the lower layer is separated, and the primary adipose tissue extract is located in the first syringe barrel ；

(4) The filtering module filters the preliminary adipose tissue extract. The specific steps are: connect the first syringe barrel containing the preliminary adipose tissue extract to the filter, and the preliminary adipose tissue extract in the first syringe barrel is passed through the filter. Filter and purify to obtain adipose tissue extract (rich in growth factors).

(5) The collection module collects the adipose tissue extract, and the specific steps are: the adipose tissue extract flows into the collector for collection.

It should be understood that, in the device of the present invention, the first syringe barrel is centrifuged, transferred to the next module, and other operations, the plug is to block the first pipe joint, and the first pipe joint and the first pipe In operations such as port connection and filtration, the plug is opened to facilitate the extraction of adipose tissue extract.

In a preferred embodiment, the adipose tissue is adipose tissue sucked by a fat extractor.

In a preferred embodiment of the present invention, the fat passes through the fat separation module, the fat emulsification module, the emulsified fat stratification module, the filtration module and the collection module in sequence to obtain the adipose tissue extract.

In another preferred embodiment, in the fat separation module, the first centrifuge can centrifuge the fat in the first syringe barrel to separate purified fat, and the purified fat is located in the first syringe barrel.

In another preferred embodiment, in the fat emulsification module, the first syringe barrel containing purified fat obtained by centrifugation of the fat separation module is connected to the first port through the first pipe joint, and the second syringe barrel passes through the second The pipe joint is connected to the second port, the emulsification cavity is connected to the third port through the first inlet pipe, and the first syringe barrel and the second syringe barrel are repeatedly beaten to purify the fat. Emulsification is carried out in the emulsification cavity to obtain nano fat, and the nano fat is located in the first syringe barrel.

In another preferred embodiment, in the emulsified fat stratification module, the first syringe barrel containing nano fat is obtained by emulsifying the fat emulsification module and centrifuged in the second centrifugal cavity of the second centrifuge to obtain the lower fat tissue. Preliminary extract of the adipose tissue, and the primary adipose tissue extract is located in the first syringe barrel.

In another preferred embodiment, in the filtering module, the emulsified fat layering module obtains a first syringe barrel containing a preliminary product of adipose tissue extract, and the first syringe barrel is connected to the filter, and the primary adipose tissue extract in the first syringe barrel passes through The filter is filtered and purified to obtain adipose tissue extract.

In another preferred embodiment, in the collection module, the collector collects the nano fat obtained by the emulsified fat layering module.

In another preferred embodiment, the fat is fat drawn from adipose tissue of animals (including humans)

In another preferred embodiment, the cleaning fluid is physiological saline

In another preferred embodiment, the storage module is a refrigerator.

In another preferred embodiment, the plug can block the first pipe joint.

In another preferred embodiment, the plug can block the second pipe joint.

In another preferred embodiment, the filter is a microporous membrane filter.

In another preferred embodiment, the microporous membrane filter includes a liquid inlet 20, a liquid outlet 21, and a microporous membrane. The liquid flows in from the liquid inlet, and is filtered through the microporous membrane from the liquid outlet. Outflow.

In another preferred embodiment, the emulsifier is a homogeneous emulsifier.

In another preferred embodiment, the collector is a collecting tube.

In another preferred embodiment, the connection is communication.

The main advantages of the present invention include:

The device of the present invention can be used simply and conveniently to extract an extract rich in growth factors from adipose tissue.

The present invention will be further explained below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. The experimental methods that do not indicate specific conditions in the following examples are usually in accordance with conventional conditions or in accordance with the conditions recommended by the manufacturer. Unless otherwise stated, percentages and parts are calculated by weight.

Example 1

Embodiment 1 provides an extraction device for adipose tissue extract, the device includes a fat separation module, a fat emulsification module, an emulsified fat stratification module, a filtration module, and a collection module;

The fat emulsification module includes an emulsifier 6, a second syringe barrel 7 and a three-way connector 8. The emulsifier includes an emulsification cavity, the emulsification cavity is connected to the first inlet pipe 9, and the second The front end of the second syringe barrel is provided with a second pipe joint 10. The three-way connector includes a first port 11, a second port 12, and a third port 13. The first syringe barrel can pass through the A pipe joint can be detachably connected with the first port, the second syringe barrel can be detachably connected with the second port through a second pipe joint, and the emulsification cavity can be detachably connected with the first port. The liquid pipe is connected with the third port;

In the adipose tissue extracting liquid extraction device of this embodiment 1, the fat passes through the fat separation module, the fat emulsification module, the emulsified fat stratification module, the filtering module and the collection module in order to obtain the adipose tissue extract.

All documents mentioned in the present invention are cited as references in this application, as if each document was individually cited as a reference. In addition, it should be understood that after reading the above teaching content of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of this application.

Claims

An extraction device for adipose tissue extraction liquid, characterized in that the device includes a fat separation module, a fat emulsification module, an emulsified fat stratification module, a filtration module and a collection module;

The fat separation module includes a first centrifuge (1) and a first syringe barrel (2). The first centrifuge includes a first centrifuge chamber (3). The first syringe barrel can be centrifuged in the first centrifuge. Centrifugation is performed in the cavity, the front end of the first syringe barrel is provided with a first pipe joint (4), and the first pipe joint and the plug (5) are detachably connected;

The fat emulsification module includes an emulsifier (6), a second syringe barrel (7) and a three-way connector (8), the emulsifier includes an emulsification cavity, the emulsification cavity and the first liquid inlet pipe ( 9) Connection, the front end of the second syringe barrel is provided with a second pipe joint (10), and the three-way connector includes a first port (11), a second port (12) and a third Through port (13), the first syringe barrel can be detachably connected to the first port through a first pipe joint, and the second syringe barrel can be connected to the second pipe through a second pipe joint. The port can be detachably connected, and the emulsification cavity can be connected to the third port through the first liquid inlet pipe;

The emulsified fat stratification module includes a second centrifuge (14), the second centrifuge includes a second centrifuge chamber (15), and the first syringe barrel can be centrifuged in the second centrifuge chamber;

The filter module includes a filter (16), and the liquid in the first syringe barrel can be filtered in the filter;

The collection module includes a collector (17), and the collector collects the adipose tissue extract filtered by the filter.
The device of claim 1, wherein the fat passes through the fat separation module, the fat emulsification module, the emulsified fat stratification module, the filtration module and the collection module in sequence to obtain the adipose tissue extract.
The device according to claim 1, wherein in the fat separation module, the first centrifuge can centrifuge the fat in the first syringe barrel to separate purified fat, and the purified fat is located in the first syringe barrel. In a syringe barrel;

In the fat emulsification module, the first syringe barrel containing purified fat obtained by centrifugation of the fat separation module is connected to the first port through a first pipe joint, and the second syringe barrel is connected to the first port through a second pipe joint. The two ports are connected, the emulsification cavity is connected to the third port through the first liquid inlet pipe, and the first syringe barrel and the second syringe barrel are repeatedly beaten to make the purified fat emulsify in the emulsification cavity. Obtain nano fat, where the nano fat is located in the first syringe barrel;

In the emulsified fat stratification module, the fat emulsification module emulsifies and obtains a first syringe barrel containing nano fat, which is centrifuged in the second centrifugal cavity of the second centrifuge to separate and obtain a preliminary product of the lower adipose tissue extract. The initial product of adipose tissue extract is located in the first syringe barrel;

In the filtering module, the emulsified fat stratification module obtains a first syringe barrel containing a primary adipose tissue extract and is connected to a filter, and the primary adipose tissue extract in the first syringe barrel is filtered and purified by the filter to obtain adipose tissue Extract

In the collecting module, the collector collects the nano fat obtained by the emulsified fat layering module.
The device according to claim 1, wherein the device further comprises a storage module, and the storage module is used to store the adipose tissue extract collected in the collector.
The device according to claim 1, wherein the first syringe barrel and the first syringe barrel comprise a push rod (18) and a piston (19), and the piston is tightly connected to the inner wall of the syringe barrel. Connected, the push rod is connected to the piston, and the push rod can push the piston to freely slide back and forth along the inner wall of the syringe barrel.
The device of claim 1, wherein the filter is a microporous membrane filter.
The device according to claim 6, characterized in that the microporous membrane filter comprises a liquid inlet (20), a liquid outlet (21) and a microporous membrane, and the liquid flows in from the liquid inlet and passes through The microporous membrane filters and flows out from the liquid outlet.
The device of claim 1, wherein the emulsifier is a homogeneous emulsifier.
The device of claim 1, wherein the inner walls of the first port, the second port and the third port are provided with coils, and the first pipe joint, the second pipe joint and The connection between the first inlet pipe and the first port, the second port and the third port is provided with threads, and the threads can be rotated and fixed on the spiral ring.
8. The device according to claim 7, wherein the collector is connected to the filtrate outlet of the filter through a conveying pipe.
The device of claim 6, wherein the pore size of the microporous filter membrane is 0.1-0.8 μm.