WO2021235158A1 - Fluorescent fingerprint image acquisition device - Google Patents

Fluorescent fingerprint image acquisition device Download PDF

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WO2021235158A1
WO2021235158A1 PCT/JP2021/016112 JP2021016112W WO2021235158A1 WO 2021235158 A1 WO2021235158 A1 WO 2021235158A1 JP 2021016112 W JP2021016112 W JP 2021016112W WO 2021235158 A1 WO2021235158 A1 WO 2021235158A1
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excitation light
wavelength
fluorescence
light
fingerprint image
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PCT/JP2021/016112
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French (fr)
Japanese (ja)
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雄樹 小野
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コニカミノルタ株式会社
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Priority to CN202180035646.1A priority Critical patent/CN115667888A/en
Priority to JP2022524339A priority patent/JPWO2021235158A1/ja
Publication of WO2021235158A1 publication Critical patent/WO2021235158A1/en

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence

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  • the present invention relates to a fluorescence fingerprint image acquisition device, and in particular, when acquiring a fluorescence fingerprint image based on a fluorescence fingerprint (fluorescence characteristic) of an object, the fluorescence spectrum data can be compressed to increase the speed, and the apparatus can be used.
  • the present invention relates to a fluorescent fingerprint image acquisition device that can be simplified in configuration and can be realized at low cost.
  • the conventional spectroscopic fluorometer uses a xenon lamp as the excitation light source, selects a specific wavelength by spectroscopy, and irradiates the target.
  • a xenon lamp as the excitation light source
  • selects a specific wavelength by spectroscopy and irradiates the target.
  • Increasing the resolution of wavelength selection has a problem that the amount of excitation light that selectively uses only a part of the emitted wavelengths decreases. There is a trade-off between the resolution of wavelength selection and the amount of light.
  • Patent Document 1 when the fluorescence spectrum data of the object is acquired as two-dimensional data by photographing with a camera, the fluorescence spectrum data per measurement becomes enormous, so that the production process or the like There is a problem that quality monitoring cannot be performed in real time. Moreover, it is not suitable as a configuration for continuous measurement at high speed.
  • the present invention has been made in view of the above problems and situations, and the problem is that when acquiring a fluorescence fingerprint image based on the fluorescence fingerprint of an object, the fluorescence spectrum data can be compressed and speeded up. Moreover, it is to provide a fluorescent fingerprint image acquisition device which can simplify the configuration of the device and realize low cost.
  • the present inventor uses a one-dimensional line sensor as a measuring unit in the process of examining the cause of the above problems, thereby compressing the fluorescence spectrum data to enable high speed and speeding up.
  • a fluorescent fingerprint image acquisition device that can reduce the cost with a device having a simple configuration, and have arrived at the present invention. That is, the above-mentioned problem according to the present invention is solved by the following means.
  • a fluorescent fingerprint image acquisition device based on the measurement of the fluorescent fingerprint of an object.
  • the object is irradiated with an excitation light source that emits light having a specific excitation light wavelength.
  • the fluorescence from the object excited by the light of the excitation light wavelength is received by a measuring unit having a one-dimensional line sensor, and the fluorescence is received.
  • a fluorescent fingerprint image acquisition device that continuously acquires a fluorescent fingerprint image by making the relative positions of the measuring unit and the object variable.
  • the excitation light wavelength, the fluorescence wavelength, and the fluorescence intensity are measured in advance as the fluorescence fingerprint of the object, and a plurality of excitation light wavelengths effective for the measurement are selected. From the excitation light source that emits the light of the plurality of selected excitation light wavelengths, the light of the plurality of selected excitation light wavelengths is sequentially emitted for each wavelength, and the object is irradiated with the light.
  • the fluorescent fingerprint image acquisition device according to item 1, wherein the measuring unit receives fluorescence from the object excited by light having each excitation light wavelength.
  • an optical filter that absorbs or reflects light having a wavelength shorter than that of the excitation light and cuts the light is provided.
  • the fluorescent fingerprint image acquisition device according to any one of items 1 to 3, wherein the optical filter is switched in synchronization with the wavelength switching of the excitation light source.
  • a plurality of the one-dimensional line sensors corresponding to the excitation light wavelength of the excitation light source are provided.
  • an optical filter that absorbs or reflects light having a wavelength shorter than the excitation light wavelength and cuts the light is provided.
  • the fluorescent fingerprint image acquisition device according to any one of items 1 to 4, wherein the one-dimensional line sensor receives light in synchronization with the switching of the excitation light source.
  • the fluorescence spectrum data can be compressed to increase the speed, and the configuration of the apparatus can be simplified, resulting in low cost.
  • a possible fluorescent fingerprint image acquisition device can be provided.
  • the present invention is based on the fact that a one-dimensional line sensor receives light emitted by sequentially irradiating an object with light having a specific excitation light wavelength at a relative position, and continuously acquires a fluorescence fingerprint image. It is a characteristic feature. That is, in the present invention, since the one-dimensional line sensor is used instead of acquiring the spectrum data to be measured as two-dimensional data by camera photography or the like, continuous processing with high resolution is easy as compared with the area sensor. .. For example, in the case of sheet-shaped defect inspection, when inspecting continuously flowing objects, it is difficult to synchronize with the area sensor, whereas with the one-dimensional line sensor, it is output for each scan. Therefore, continuous processing becomes easy.
  • the object is sequentially irradiated with light having a specific excitation light wavelength, the fluorescence from the object is received by a measuring unit having a one-dimensional line sensor, and a fluorescent fingerprint image is continuously acquired.
  • a measuring unit having a one-dimensional line sensor it is possible to compress the fluorescence spectrum data to increase the speed, and it is possible to realize cost reduction with a device having a simple configuration.
  • FIG. 1 Flow chart showing the process of selecting multiple excitation light wavelengths that are effective for user measurement
  • Schematic diagram of the fluorescent fingerprint image acquisition device of the present invention A flowchart showing a control procedure by a processing unit executed by a fluorescent fingerprint image acquisition device.
  • the fluorescent fingerprint image acquisition device of the present invention is a fluorescent fingerprint image acquisition device based on the measurement of the fluorescent fingerprint of the object, and is a fluorescent fingerprint image acquisition device based on the measurement of the fluorescent fingerprint of the object.
  • Is irradiated from an excitation light source that emits light having a specific excitation light wavelength, and fluorescence from the object excited by the light having the excitation light wavelength is received by a measuring unit having a one-dimensional line sensor, and the measuring unit receives the fluorescence. It is characterized in that the relative position of the object is variable and fluorescent fingerprint images are continuously acquired. This feature is a technical feature common to or corresponding to each of the following embodiments.
  • the excitation light wavelength, the fluorescence wavelength, and the fluorescence intensity are measured in advance as the fluorescence fingerprint of the object, a plurality of excitation light wavelengths effective for the measurement are selected, and the plurality of selected excitation light wavelengths are selected. From the excitation light source that emits the light of the excitation light wavelength, the light of the plurality of selected excitation light wavelengths is sequentially emitted for each wavelength, the object is irradiated, and the light excited by the light of each of the excitation light wavelengths is used. It is preferable that the fluorescence from the object is received by the measuring unit. Therefore, first, in the measurement for acquiring the target fluorescent fingerprint image, it is not necessary to scan the entire wavelength range for the measurement, so that the fluorescent fingerprint information can be obtained by the measurement in a short time.
  • the excitation light source is a super-continue light source or an LED in terms of increasing the amount of light, eliminating the need for an optical filter switching mechanism in the light source unit, and simplifying and downsizing the device. ..
  • Stray light can be removed by providing an optical filter in front of the one-dimensional line sensor that absorbs or reflects and cuts light having a wavelength shorter than that of the excitation light, and switching the optical filter in synchronization with the wavelength switching of the excitation light source. It is preferable in terms of points.
  • An optic having a plurality of the one-dimensional line sensors corresponding to the excitation light wavelength of the excitation light source and absorbing or reflecting and cutting light having a wavelength shorter than the excitation light wavelength in front of each one-dimensional line sensor. It is preferable to provide a filter and receive light in synchronization with the switching of the excitation light source because the switching of the optical filter becomes unnecessary, the light receiving unit is simplified and downsized, and the measurement time is shortened. ..
  • the fluorescence fingerprint image acquisition device of the present invention is a fluorescence fingerprint image acquisition device based on the measurement of the fluorescence fingerprint of an object, and the object is irradiated with light of a specific excitation light wavelength from an excitation light source to emit the light.
  • the fluorescence from the object excited by the light of the excitation light wavelength is received by the measuring unit having a one-dimensional line sensor, the relative position between the measuring unit and the object is made variable, and the fluorescence fingerprint image is continuously obtained. get.
  • the excitation light wavelength, the fluorescence wavelength and the fluorescence intensity are measured in advance as the fluorescence fingerprint of the object, a plurality of excitation light wavelengths effective for the measurement are selected, and the plurality of selected excitation lights are selected. From an excitation light source that emits light of a wavelength, a plurality of selected lights of the excitation light wavelength are sequentially emitted for each wavelength, the object is irradiated with the light, and the object is excited by the light of each excitation light wavelength. It is preferable that the fluorescence from the above is received by the measuring unit.
  • Applications of the fluorescent fingerprint image acquisition device of the present invention include, for example, quality control of sheet-shaped products, detection of foreign substances, guarantee of producers of agricultural products, and authenticity determination of branded products (printed on tags using special paint). , Environmental inspection (water quality, soil, pesticides), agricultural use (disease judgment), etc.
  • Examples of the object include an object according to the above-mentioned use, and examples thereof include sheet-like products such as paper, film, and printed matter, agricultural products, branded products, and samples for environmental inspection and agriculture.
  • FIG. 1 is a flowchart showing a process of measuring a fluorescent fingerprint of an object in advance and selecting a plurality of excitation light wavelengths effective for the measurement.
  • the wavelength is changed in advance in a wide wavelength range, and the excitation light wavelength, the fluorescence wavelength, and the fluorescence intensity are measured as the fluorescence fingerprint (EEM) of the object (step S1).
  • EEM fluorescence fingerprint
  • a general spectrofluorescence fluorometer can be used, for example, a spectrofluorescence fluorometer FP-8000 (manufactured by Nippon Spectral Co., Ltd.) and a spectrofluorescence fluorometer F-7100 (Hitachi).
  • FIGS. 2A and 2B are graphs before and after heat is applied to a certain resin material, and a difference can be seen in the fluorescent fingerprints due to the heat applied.
  • the fluorescent fingerprint data is analyzed by principal component analysis (step S2).
  • the analysis is a multivariate analysis and is processed by a general personal computer.
  • a plurality of effective excitation light wavelengths used for determination are selected (step S3).
  • the contribution of the fluorescence wavelength can be obtained.
  • the fluorescence of a certain wavelength ⁇ 1 is detected, the substance A is included, and when the fluorescence of a certain wavelength ⁇ 2 is detected, the substance B is included.
  • the fluorescence spectrum becomes characteristic, and the result can be used to determine the inclusion of foreign matter. From the contribution of such fluorescence wavelengths, the user selects a plurality of desired excitation light wavelengths effective for measurement.
  • a fluorescent fingerprint image is acquired using the fluorescent fingerprint image acquisition device shown in FIG. 1
  • FIG. 3 is a schematic diagram of the fluorescent fingerprint image acquisition device of the present invention.
  • the fluorescent fingerprint image acquisition device 100 of the present invention is a device that acquires a fluorescent fingerprint image based on the fluorescent fingerprint of the object 200, and is at least an excitation light source 101, a measuring unit 102, an optical filter 103, and a carrier. It includes a mechanism 104 and a processing unit 105.
  • the excitation light source irradiates the object with light having a plurality of excitation light wavelengths selected in advance in the wavelength selection step to generate fluorescence from the components of the object.
  • it is a super-continue light source (a wideband pulse light source that emits strong light with a uniform phase over a very wide wavelength range by utilizing the non-linear effect of an optical fiber, and is also called an "SC light source") or an LED light source. And so on.
  • SC light source wideband pulse light source that emits strong light with a uniform phase over a very wide wavelength range by utilizing the non-linear effect of an optical fiber, and is also called an "SC light source”
  • LED light source LED light source.
  • the specific range of the excitation light wavelength is preferably within the range of visible light.
  • the wavelength of the excitation light emitted from the excitation light source can be arbitrarily changed by inputting a plurality of excitation light wavelengths effective for the measurement selected in advance by the user using the keyboard, mouse, or the like of the processing unit. It is possible.
  • the transport mechanism may have a mechanism capable of transporting an object, for example, an endless belt may be moved around by a roller, a measured object may be directly moved by a roller, or a roller may be used. May also move the object to be measured along the surface of a large cylindrical rotating drum.
  • the measuring unit receives the fluorescence of the pattern peculiar to the component emitted from the object by being irradiated with the excitation light, measures the excitation light wavelength, the fluorescence wavelength and the fluorescence intensity from the received fluorescence, and measures the fluorescence fingerprint data. Is sent to the processing unit.
  • the measuring unit includes a one-dimensional line sensor and an optical filter provided in front of the one-dimensional line sensor to absorb or reflect and cut light having a wavelength shorter than that of the excitation light.
  • a plurality of image pickup devices are arranged over the width of an object. Then, by moving the object in the transport direction, the one-dimensional line sensor can take a two-dimensional image on the object.
  • the one-dimensional line sensor examples include a CCD sensor (Charge Coupled Device) and a CMOS sensor (Complementary Metal Oxide Semiconductor).
  • the one-dimensional line sensor performs an image pickup operation in each image pickup device, which outputs a charge amount and a voltage according to the amount of fluorescence light input to the light receiving element from the surface of the object via an optical system (lens).
  • the one-dimensional line sensor is capable of imaging in a plurality of wavelength bands, and transmits the captured fluorescent fingerprint data to the processing unit.
  • the optical filter is controlled to switch to a desired optical filter that absorbs or reflects and cuts light having a wavelength shorter than the excitation light wavelength emitted from the excitation light source in synchronization with the wavelength switching of the excitation light source.
  • the optical filter include a cutoff filter, a bandpass filter (BPF, an interference filter), an acoustic optical wavelength variable filter (AOTF), a liquid crystal tunable filter and the like.
  • BPF bandpass filter
  • AOTF acoustic optical wavelength variable filter
  • a plurality of the one-dimensional line sensors may be provided corresponding to the excitation light wavelength of the excitation light source, and an optical filter may also be provided in front of the one-dimensional line sensor.
  • the processing unit is a device that acquires a fluorescent fingerprint image by processing abnormality detection and accompanying warnings and occurrence records from the fluorescent fingerprint data acquired by the measurement unit, and also performs the entire operation of the fluorescent fingerprint image acquisition device. Is centrally controlled.
  • a processing unit for example, a general personal computer or the like is used.
  • a dedicated integrated circuit ASIC: ASIC
  • the processing unit includes a memory, a control unit, a calculation processing unit, and the like.
  • the user can input measurement processing conditions and the like into the processing unit using the keyboard and mouse.
  • FIG. 4 is a flowchart showing a control procedure by the processing unit executed by the fluorescent fingerprint image acquisition device.
  • the excitation light source is switched to irradiate the first excitation light wavelength (step S11).
  • the optical filter of the measuring unit is also switched to an optical filter that cuts light having a wavelength shorter than the wavelength of the excitation light emitted from the excitation light source (step S12).
  • the object is irradiated with the excitation light from the switched excitation light source (step S13).
  • the irradiation time, irradiation intensity, etc. of the excitation light are appropriately set according to the object. Further, at this time, it is preferable that the object is transported so as to be irradiated at a desired position by the transport mechanism.
  • the object is irradiated with excitation light, the fluorescence from the object excited by the light having the excitation light wavelength is received by the measuring unit (step S14), and the wavelength and intensity of the received fluorescence are measured.
  • the fluorescence fingerprint data consisting of the fluorescence wavelength, the fluorescence intensity and the excitation light wavelength is transmitted to the processing unit.
  • step S15 If the irradiation of the set plurality of excitation light wavelengths is not completed (step S15; No), the light source is switched to the excitation light source that emits the next excitation light wavelength among the selected plurality of excitation light wavelengths (step S11). For example, when a plurality of selected excitation light wavelengths are two wavelengths of ⁇ 1 and ⁇ 2, a cycle of first irradiating with an excitation light source having a wavelength of ⁇ 1 and then irradiating with an excitation light source having a wavelength of ⁇ 2 is performed.
  • step S15 when all the irradiation of the set plurality of excitation light wavelengths is completed (step S15; Yes), the following determination process is performed based on the fluorescent fingerprint image created from the fluorescent fingerprint data transmitted to the processing unit, and the determination process is performed. Record the result (step S16).
  • the determination process includes, for example, the patterns of the determination process shown in the following (1) to (3), but the determination process is not limited thereto.
  • (1) It is determined whether or not a specific fluorescence wavelength is detected when a certain excitation light wavelength is irradiated. This is a case where the fluorescence characteristics peculiar to the substance to be detected are known in advance by a pattern simply determined by the presence or absence of a specific fluorescence wavelength.
  • (2) When a certain excitation light wavelength is irradiated, a specific fluorescence wavelength is detected, and it is determined whether or not the amount of light is equal to or greater than the threshold value.
  • step S17; No After performing the determination process as described above, if no abnormality is detected (step S17; No), the process ends.
  • an abnormality is detected (step S17; Yes)
  • a warning is issued by display or voice (step S18)
  • an abnormality occurrence record such as the place and time when the abnormality occurred and what kind of abnormality is taken is taken (step S18).
  • Step S19 the process is terminated.
  • the abnormality include cases where the fluorescence intensity ratio exceeds the set range, or fluorescence is generated from a place where fluorescence is not originally generated.
  • the fluorescent fingerprint of the object is measured in advance, a plurality of excitation light wavelengths effective for the measurement are selected from the measurement results, and the light is selected from the excitation light sources that emit light of the selected plurality of excitation light wavelengths. It was said that light with multiple excitation light wavelengths would be emitted sequentially for each wavelength and received by a one-dimensional line sensor to continuously acquire fluorescent fingerprint images, but effective excitation by measuring the fluorescent fingerprint of the object in advance. Without performing the step of selecting the light wavelength, the light of a specific excitation light wavelength is simply emitted to the object, the fluorescence from the excited object is received by the one-dimensional line sensor, and the fluorescence fingerprint is continuously fluorescent.
  • a transport mechanism for transporting the object is provided and the object is moved with respect to the measurement unit.
  • a transport mechanism for transporting the measurement unit is provided and the measurement unit is provided. May be configured to move with respect to the object.
  • the fluorescent fingerprint image acquisition device of the present embodiment when acquiring a fluorescent fingerprint image based on the fluorescent fingerprint of the object, a plurality of effective wavelengths related to the quality of the object are selected in advance. Moreover, by using a one-dimensional line sensor as a measuring unit, it is possible to compress spectral data to increase the speed, and it is possible to reduce the cost with a device having a simple configuration.
  • a resin molded product A to which an ultraviolet absorber was added and a resin molded product B to which an ultraviolet absorber was not added were prepared. Then, for each of these resin molded products A and B, the fluorescent fingerprints before and after heating were measured using the fluorescent fingerprint image acquisition device of the present invention, and the results of principal component analysis of the fluorescent fingerprint data are shown in FIG.
  • the excitation light wavelength at the time of measurement was 250 to 700 nm in 10 nm increments. As shown in FIG. 5, it can be seen that the presence / absence of the ultraviolet absorber and the presence / absence of heating can be separated by the axes of the main component 2 and the main component 3.
  • the fluorescence spectrum data when acquiring a fluorescence fingerprint image based on a fluorescence fingerprint (fluorescence characteristic) of an object, the fluorescence spectrum data can be compressed to increase the speed, and the configuration of the apparatus can be simplified, resulting in low cost. It can be used in a feasible fluorescent fingerprint image acquisition device.

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Abstract

A fluorescent fingerprint image acquisition device according to the present invention is based on a measurement of a fluorescent fingerprint of an object, said device being characterized in that the object is irradiated with light of a specific excitation light wavelength emitted by an excitation light source, fluorescent light that is from the object and excited by the light of the excitation light wavelength is received by a measurement unit having a one-dimensional line sensor, the relative positions of the measurement unit and the object are variable, and fluorescent light fingerprint images are continuously acquired.

Description

蛍光指紋画像取得装置Fluorescent fingerprint image acquisition device
 本発明は、蛍光指紋画像取得装置に関し、特に、対象物の蛍光指紋(蛍光特性)に基づく蛍光指紋画像を取得する際に、蛍光スペクトルデータを圧縮して高速化が可能で、かつ、装置の構成も簡略化でき、低コストが実現可能な蛍光指紋画像取得装置に関する。 The present invention relates to a fluorescence fingerprint image acquisition device, and in particular, when acquiring a fluorescence fingerprint image based on a fluorescence fingerprint (fluorescence characteristic) of an object, the fluorescence spectrum data can be compressed to increase the speed, and the apparatus can be used. The present invention relates to a fluorescent fingerprint image acquisition device that can be simplified in configuration and can be realized at low cost.
 従来、光吸収特性や蛍光特性などの測定により、評価対象物の成分及びその状態などを分析又は判別・判定する等の手法が種々開発されている。
 しかし、一般に、蛍光測定は、吸光光度法による測定に比べて検出感度が優れていると考えられる。
 このような蛍光測定を用いて、対象物に照射する励起光波長(励起波長)(nm)と、対象物から発する発光の蛍光波長(nm)と、当該蛍光波長における対象物の蛍光強度を用いて、3軸からなる3次元の蛍光スペクトルデータとし解析する手法が知られている。このような蛍光スペクトルデータを等高線状に表したグラフは、「蛍光指紋」と呼ばれ、「励起蛍光マトリクス(EEM:Excitation-Emission Matrix)」とも呼ばれる。
Conventionally, various methods have been developed for analyzing, discriminating, and determining the components of the evaluation target and their states by measuring the light absorption characteristics and the fluorescence characteristics.
However, in general, it is considered that the fluorescence measurement is superior in detection sensitivity to the measurement by the absorptiometry.
Using such fluorescence measurement, the excitation light wavelength (excitation wavelength) (nm) to irradiate the object, the fluorescence wavelength (nm) of the emission emitted from the object, and the fluorescence intensity of the object at the fluorescence wavelength are used. Therefore, a method of analyzing as three-dimensional fluorescence spectrum data composed of three axes is known. A graph representing such fluorescence spectrum data in a contour line is called a "fluorescence fingerprint" and is also called an "excited fluorescence matrix (EEM)".
 従来の分光蛍光光度計は、励起光源にキセノンランプを用い、分光によって特定の波長を選択し対象に照射している。波長選択の分解能を上げると、発光している一部の波長のみを選択的に利用する励起光の光量が低下してしまうという問題がある。波長選択の分解能と光量はトレードオフの関係になっている。 The conventional spectroscopic fluorometer uses a xenon lamp as the excitation light source, selects a specific wavelength by spectroscopy, and irradiates the target. Increasing the resolution of wavelength selection has a problem that the amount of excitation light that selectively uses only a part of the emitted wavelengths decreases. There is a trade-off between the resolution of wavelength selection and the amount of light.
 特に、広い面積を測定する場合には広範囲を励起光で照射する必要があるため、光量不足の問題が顕著になる。
 また、特許文献1に開示されているように、対象物の蛍光スペクトルデータをカメラ撮影により2次元データとして取得している場合には、1測定当たりの蛍光スペクトルデータが膨大になるため生産工程などでリアルタイムに品質のモニタリングを行えないという問題がある。また、高速に連続測定するための構成としては不向きである。
In particular, when measuring a large area, it is necessary to irradiate a wide area with excitation light, so that the problem of insufficient light amount becomes remarkable.
Further, as disclosed in Patent Document 1, when the fluorescence spectrum data of the object is acquired as two-dimensional data by photographing with a camera, the fluorescence spectrum data per measurement becomes enormous, so that the production process or the like There is a problem that quality monitoring cannot be performed in real time. Moreover, it is not suitable as a configuration for continuous measurement at high speed.
特許第5985709号公報Japanese Patent No. 5985709
 本発明は、上記問題・状況に鑑みてなされたものであり、その解決課題は、対象物の蛍光指紋に基づく蛍光指紋画像を取得する際に、蛍光スペクトルデータを圧縮して高速化が可能で、かつ、装置の構成も簡略化でき、低コストが実現可能な蛍光指紋画像取得装置を提供することである。 The present invention has been made in view of the above problems and situations, and the problem is that when acquiring a fluorescence fingerprint image based on the fluorescence fingerprint of an object, the fluorescence spectrum data can be compressed and speeded up. Moreover, it is to provide a fluorescent fingerprint image acquisition device which can simplify the configuration of the device and realize low cost.
 本発明者は、上記課題を解決すべく、上記問題の原因等について検討する過程において、測定部として一次元ラインセンサーを用いることで、蛍光スペクトルデータを圧縮して高速化を可能とし、かつ、簡易な構成の装置で、低コスト化を図ることができる蛍光指紋画像取得装置を提供できることを見いだし本発明に至った。
 すなわち、本発明に係る上記課題は、以下の手段により解決される。
In order to solve the above problems, the present inventor uses a one-dimensional line sensor as a measuring unit in the process of examining the cause of the above problems, thereby compressing the fluorescence spectrum data to enable high speed and speeding up. We have found that we can provide a fluorescent fingerprint image acquisition device that can reduce the cost with a device having a simple configuration, and have arrived at the present invention.
That is, the above-mentioned problem according to the present invention is solved by the following means.
 1.対象物の蛍光指紋の測定に基づく蛍光指紋画像取得装置であって、
 前記対象物に、特定の励起光波長の光を出射する励起光源より照射し、
 前記励起光波長の光によって励起された前記対象物からの蛍光を、一次元ラインセンサーを有する測定部により受光し、
 前記測定部と前記対象物の相対位置を可変とし、連続的に蛍光指紋画像を取得する蛍光指紋画像取得装置。
1. 1. A fluorescent fingerprint image acquisition device based on the measurement of the fluorescent fingerprint of an object.
The object is irradiated with an excitation light source that emits light having a specific excitation light wavelength.
The fluorescence from the object excited by the light of the excitation light wavelength is received by a measuring unit having a one-dimensional line sensor, and the fluorescence is received.
A fluorescent fingerprint image acquisition device that continuously acquires a fluorescent fingerprint image by making the relative positions of the measuring unit and the object variable.
 2.あらかじめ前記対象物の前記蛍光指紋として、励起光波長、蛍光波長及び蛍光強度を測定しておき、測定に有効な励起光波長を複数選択し、
 選択した複数の前記励起光波長の光を出射する励起光源より、前記選択した複数の前記励起光波長の光を波長ごとに順次発光させ、前記対象物に照射し、
 各前記励起光波長の光によって励起された前記対象物からの蛍光を、前記測定部により受光する第1項に記載の蛍光指紋画像取得装置。
2. 2. The excitation light wavelength, the fluorescence wavelength, and the fluorescence intensity are measured in advance as the fluorescence fingerprint of the object, and a plurality of excitation light wavelengths effective for the measurement are selected.
From the excitation light source that emits the light of the plurality of selected excitation light wavelengths, the light of the plurality of selected excitation light wavelengths is sequentially emitted for each wavelength, and the object is irradiated with the light.
The fluorescent fingerprint image acquisition device according to item 1, wherein the measuring unit receives fluorescence from the object excited by light having each excitation light wavelength.
 3.前記励起光源が、スーパーコンティニューム光源又はLEDである第1項又は第2項に記載の蛍光指紋画像取得装置。 3. The fluorescent fingerprint image acquisition device according to item 1 or 2, wherein the excitation light source is a super-continue light source or an LED.
 4.前記一次元ラインセンサーの前に、励起光より短い波長の光を吸収又は反射しカットする光学フィルターを備え、
 前記励起光源の波長切り替えに同期して前記光学フィルターを切り替える第1項から第3項までのいずれか一項に記載の蛍光指紋画像取得装置。
4. In front of the one-dimensional line sensor, an optical filter that absorbs or reflects light having a wavelength shorter than that of the excitation light and cuts the light is provided.
The fluorescent fingerprint image acquisition device according to any one of items 1 to 3, wherein the optical filter is switched in synchronization with the wavelength switching of the excitation light source.
 5.前記励起光源の前記励起光波長に対応して複数の前記一次元ラインセンサーを備え、
 各当該一次元ラインセンサーの前に、それぞれ前記励起光波長より短い波長の光を吸収又は反射しカットする光学フィルターを備え、
 当該一次元ラインセンサーを前記励起光源の切り替えに同期させて受光する第1項から第4項までのいずれか一項に記載の蛍光指紋画像取得装置。
5. A plurality of the one-dimensional line sensors corresponding to the excitation light wavelength of the excitation light source are provided.
In front of each one-dimensional line sensor, an optical filter that absorbs or reflects light having a wavelength shorter than the excitation light wavelength and cuts the light is provided.
The fluorescent fingerprint image acquisition device according to any one of items 1 to 4, wherein the one-dimensional line sensor receives light in synchronization with the switching of the excitation light source.
 本発明の上記手段により、対象物の蛍光指紋に基づく蛍光指紋画像を取得する際に、蛍光スペクトルデータを圧縮して高速化が可能で、かつ、装置の構成も簡略化でき、低コストが実現可能な蛍光指紋画像取得装置を提供することができる。 By the above means of the present invention, when acquiring a fluorescence fingerprint image based on a fluorescence fingerprint of an object, the fluorescence spectrum data can be compressed to increase the speed, and the configuration of the apparatus can be simplified, resulting in low cost. A possible fluorescent fingerprint image acquisition device can be provided.
 本発明の効果の発現機構又は作用機構については、明確にはなっていないが、以下のように推察している。
 本発明は、特定の励起光波長の光を順次対象物に相対的位置を変えて照射し発せられた蛍光を、一次元ラインセンサーにより受光し、連続的に蛍光指紋画像を取得することを基本的特徴としている。
 すなわち、本発明では、測定対象のスペクトルデータをカメラ撮影等により二次元データとして取得するのではなく、一次元ラインセンサーを用いるので、エリアセンサーに比較して、高分解能で連続処理が容易である。例えば、シート状の欠陥検査の場合には、連続して流れている対象物を検査するとき、エリアセンサーでは同期がとりにくいのに対して、一次元ラインセンサーでは、1スキャン毎に出力されるため、連続的な処理が容易となる。
Although the mechanism of expression or mechanism of action of the effect of the present invention has not been clarified, it is inferred as follows.
The present invention is based on the fact that a one-dimensional line sensor receives light emitted by sequentially irradiating an object with light having a specific excitation light wavelength at a relative position, and continuously acquires a fluorescence fingerprint image. It is a characteristic feature.
That is, in the present invention, since the one-dimensional line sensor is used instead of acquiring the spectrum data to be measured as two-dimensional data by camera photography or the like, continuous processing with high resolution is easy as compared with the area sensor. .. For example, in the case of sheet-shaped defect inspection, when inspecting continuously flowing objects, it is difficult to synchronize with the area sensor, whereas with the one-dimensional line sensor, it is output for each scan. Therefore, continuous processing becomes easy.
 したがって、本発明のように、特定の励起光波長の光を順次対象物に照射し、対象物からの蛍光を一次元ラインセンサーを有する測定部により受光し、連続的に蛍光指紋画像を取得することにより、蛍光スペクトルデータを圧縮して高速化を可能とし、かつ、簡易な構成の装置で、低コスト化を実現することができる。 Therefore, as in the present invention, the object is sequentially irradiated with light having a specific excitation light wavelength, the fluorescence from the object is received by a measuring unit having a one-dimensional line sensor, and a fluorescent fingerprint image is continuously acquired. As a result, it is possible to compress the fluorescence spectrum data to increase the speed, and it is possible to realize cost reduction with a device having a simple configuration.
ユーザーによる測定に有効な励起光波長を複数選択する工程を示すフローチャートFlow chart showing the process of selecting multiple excitation light wavelengths that are effective for user measurement 励起光波長、蛍光波長及び蛍光強度からなる蛍光指紋の一例を示す図The figure which shows an example of the fluorescence fingerprint which consists of an excitation light wavelength, a fluorescence wavelength and a fluorescence intensity. 励起光波長、蛍光波長及び蛍光強度からなる蛍光指紋の一例を示す図The figure which shows an example of the fluorescence fingerprint which consists of an excitation light wavelength, a fluorescence wavelength and a fluorescence intensity. 本発明の蛍光指紋画像取得装置の模式図Schematic diagram of the fluorescent fingerprint image acquisition device of the present invention 蛍光指紋画像取得装置で実行される処理部による制御手順を示すフローチャートA flowchart showing a control procedure by a processing unit executed by a fluorescent fingerprint image acquisition device. 本発明の蛍光指紋画像取得装置を用いて、加熱前後の蛍光指紋を測定し、蛍光指紋データを主成分分析した結果を示す図The figure which shows the result of having measured the fluorescent fingerprint before and after heating using the fluorescent fingerprint image acquisition apparatus of this invention, and performing the principal component analysis of the fluorescent fingerprint data.
 本発明の蛍光指紋画像取得装置は、対象物の蛍光指紋の測定に基づく蛍光指紋画像取得装置であって、対象物の蛍光指紋の測定に基づく蛍光指紋画像取得装置であって、前記対象物に、特定の励起光波長の光を出射する励起光源より照射し、前記励起光波長の光によって励起された前記対象物からの蛍光を、一次元ラインセンサーを有する測定部により受光し、前記測定部と前記対象物の相対位置を可変とし、連続的に蛍光指紋画像を取得することを特徴とする。
 この特徴は、下記各実施形態に共通又は対応する技術的特徴である。
The fluorescent fingerprint image acquisition device of the present invention is a fluorescent fingerprint image acquisition device based on the measurement of the fluorescent fingerprint of the object, and is a fluorescent fingerprint image acquisition device based on the measurement of the fluorescent fingerprint of the object. Is irradiated from an excitation light source that emits light having a specific excitation light wavelength, and fluorescence from the object excited by the light having the excitation light wavelength is received by a measuring unit having a one-dimensional line sensor, and the measuring unit receives the fluorescence. It is characterized in that the relative position of the object is variable and fluorescent fingerprint images are continuously acquired.
This feature is a technical feature common to or corresponding to each of the following embodiments.
 本発明の実施態様としては、あらかじめ前記対象物の前記蛍光指紋として、励起光波長、蛍光波長及び蛍光強度を測定しておき、測定に有効な励起光波長を複数選択し、選択した複数の前記励起光波長の光を出射する励起光源より、前記選択した複数の前記励起光波長の光を波長ごとに順次発光させ、前記対象物に照射し、各前記励起光波長の光によって励起された前記対象物からの蛍光を、前記測定部により受光することが好ましい。したがって、まず、目的とする蛍光指紋画像を取得するための測定に際し、全波長範囲を走査して測定する必要がないので、短時間の測定で、蛍光指紋情報が得られる。 In an embodiment of the present invention, the excitation light wavelength, the fluorescence wavelength, and the fluorescence intensity are measured in advance as the fluorescence fingerprint of the object, a plurality of excitation light wavelengths effective for the measurement are selected, and the plurality of selected excitation light wavelengths are selected. From the excitation light source that emits the light of the excitation light wavelength, the light of the plurality of selected excitation light wavelengths is sequentially emitted for each wavelength, the object is irradiated, and the light excited by the light of each of the excitation light wavelengths is used. It is preferable that the fluorescence from the object is received by the measuring unit. Therefore, first, in the measurement for acquiring the target fluorescent fingerprint image, it is not necessary to scan the entire wavelength range for the measurement, so that the fluorescent fingerprint information can be obtained by the measurement in a short time.
 また、前記励起光源が、スーパーコンティニューム光源又はLEDであることが、光量を大きくすることができ、また、光源部において光学フィルターの切り替え機構が不要となり装置の簡易化や小型化の点で好ましい。 Further, it is preferable that the excitation light source is a super-continue light source or an LED in terms of increasing the amount of light, eliminating the need for an optical filter switching mechanism in the light source unit, and simplifying and downsizing the device. ..
 前記一次元ラインセンサーの前に、励起光より短い波長の光を吸収又は反射しカットする光学フィルターを備え、前記励起光源の波長切り替えに同期して前記光学フィルターを切り替えることが、迷光を除去できる点で好ましい。 Stray light can be removed by providing an optical filter in front of the one-dimensional line sensor that absorbs or reflects and cuts light having a wavelength shorter than that of the excitation light, and switching the optical filter in synchronization with the wavelength switching of the excitation light source. It is preferable in terms of points.
 前記励起光源の前記励起光波長に対応して複数の前記一次元ラインセンサーを備え、各当該一次元ラインセンサーの前に、それぞれ前記励起光波長より短い波長の光を吸収又は反射しカットする光学フィルターを備え、当該一次元ラインセンサーを前記励起光源の切り替えに同期させて受光することが、光学フィルターの切り替えが不要となり、受光部の簡素化や小型化、さらに測定時間の短縮の点で好ましい。 An optic having a plurality of the one-dimensional line sensors corresponding to the excitation light wavelength of the excitation light source and absorbing or reflecting and cutting light having a wavelength shorter than the excitation light wavelength in front of each one-dimensional line sensor. It is preferable to provide a filter and receive light in synchronization with the switching of the excitation light source because the switching of the optical filter becomes unnecessary, the light receiving unit is simplified and downsized, and the measurement time is shortened. ..
 以下、本発明とその構成要素及び本発明を実施するための形態・態様について説明をする。なお、本願において、「~」は、その前後に記載される数値を下限値及び上限値として含む意味で使用する。 Hereinafter, the present invention, its constituent elements, and modes and embodiments for carrying out the present invention will be described. In addition, in this application, "-" is used in the sense that the numerical values described before and after it are included as the lower limit value and the upper limit value.
[本発明の蛍光指紋画像取得装置の概要]
 本発明の蛍光指紋画像取得装置は、対象物の蛍光指紋の測定に基づく蛍光指紋画像取得装置であって、前記対象物に、特定の励起光波長の光を出射する励起光源より照射し、前記励起光波長の光によって励起された前記対象物からの蛍光を、一次元ラインセンサーを有する測定部により受光し、前記測定部と前記対象物の相対位置を可変とし、連続的に蛍光指紋画像を取得する。
 特に、本発明では、あらかじめ前記対象物の前記蛍光指紋として、励起光波長、蛍光波長及び蛍光強度を測定しておき、測定に有効な励起光波長を複数選択し、選択した複数の前記励起光波長の光を出射する励起光源より、前記選択した複数の前記励起光波長の光を波長ごとに順次発光させ、前記対象物に照射し、各前記励起光波長の光によって励起された前記対象物からの蛍光を、前記測定部により受光することが好ましい。
[Overview of Fluorescent Fingerprint Image Acquisition Device of the Present Invention]
The fluorescence fingerprint image acquisition device of the present invention is a fluorescence fingerprint image acquisition device based on the measurement of the fluorescence fingerprint of an object, and the object is irradiated with light of a specific excitation light wavelength from an excitation light source to emit the light. The fluorescence from the object excited by the light of the excitation light wavelength is received by the measuring unit having a one-dimensional line sensor, the relative position between the measuring unit and the object is made variable, and the fluorescence fingerprint image is continuously obtained. get.
In particular, in the present invention, the excitation light wavelength, the fluorescence wavelength and the fluorescence intensity are measured in advance as the fluorescence fingerprint of the object, a plurality of excitation light wavelengths effective for the measurement are selected, and the plurality of selected excitation lights are selected. From an excitation light source that emits light of a wavelength, a plurality of selected lights of the excitation light wavelength are sequentially emitted for each wavelength, the object is irradiated with the light, and the object is excited by the light of each excitation light wavelength. It is preferable that the fluorescence from the above is received by the measuring unit.
 本発明の蛍光指紋画像取得装置の用途としては、例えば、シート状製品等の品質管理や異物検出、農作物の生産者保証、ブランド品の真贋判定(タグに特殊塗料を使って印字しておく)、環境検査(水質、土壌、農薬)、農業用途(病気判定)等が挙げられる。
 前記対象物としては、前記した用途に応じた対象物が挙げられ、例えば、紙、フィルム、印刷物等のシート状製品、農作物、ブランド品、環境検査や農業用の試料等が挙げられる。
Applications of the fluorescent fingerprint image acquisition device of the present invention include, for example, quality control of sheet-shaped products, detection of foreign substances, guarantee of producers of agricultural products, and authenticity determination of branded products (printed on tags using special paint). , Environmental inspection (water quality, soil, pesticides), agricultural use (disease judgment), etc.
Examples of the object include an object according to the above-mentioned use, and examples thereof include sheet-like products such as paper, film, and printed matter, agricultural products, branded products, and samples for environmental inspection and agriculture.
 図1は、あらかじめ対象物の蛍光指紋を測定しておき、測定に有効な励起光波長を複数選択する工程を示すフローチャートである。
 まず、あらかじめ広域波長範囲において波長を変化させて、対象物の蛍光指紋(EEM)として、励起光波長、蛍光波長及び蛍光強度を測定する(ステップS1)。ここで、蛍光指紋を測定するためには、一般的な分光蛍光光度計を用いることができ、例えば、分光蛍光光度計FP-8000(日本分光社製)、分光蛍光光度計F-7100(日立ハイテクサイエンス社製)等が挙げられる。これらの分光蛍光光度計を用いて、例えば、図2A及び図2Bに示すような励起光波長、蛍光波長及び蛍光強度からなる蛍光指紋を得る。図2A及び図2Bに示す蛍光指紋は、ある樹脂材料に熱を加えた前後のグラフであり、熱を加えたことによって蛍光指紋に違いが見られる。
FIG. 1 is a flowchart showing a process of measuring a fluorescent fingerprint of an object in advance and selecting a plurality of excitation light wavelengths effective for the measurement.
First, the wavelength is changed in advance in a wide wavelength range, and the excitation light wavelength, the fluorescence wavelength, and the fluorescence intensity are measured as the fluorescence fingerprint (EEM) of the object (step S1). Here, in order to measure the fluorescence fingerprint, a general spectrofluorescence fluorometer can be used, for example, a spectrofluorescence fluorometer FP-8000 (manufactured by Nippon Spectral Co., Ltd.) and a spectrofluorescence fluorometer F-7100 (Hitachi). (Made by High-Tech Science), etc. These spectral fluorometers are used to, for example, obtain fluorescent fingerprints consisting of excitation light wavelengths, fluorescence wavelengths and fluorescence intensities as shown in FIGS. 2A and 2B. The fluorescent fingerprints shown in FIGS. 2A and 2B are graphs before and after heat is applied to a certain resin material, and a difference can be seen in the fluorescent fingerprints due to the heat applied.
 次に、主成分分析による蛍光指紋のデータの解析を行う(ステップS2)。当該解析は、多変量解析であり、一般的なパソコンで処理を行う。 Next, the fluorescent fingerprint data is analyzed by principal component analysis (step S2). The analysis is a multivariate analysis and is processed by a general personal computer.
 次に、判定に用いる有効な励起光波長を複数選択する(ステップS3)。前記主成分分析の結果、蛍光波長の寄与度が得られる。具体的には、ある波長λ1の蛍光が検出される場合は物質Aを含み、ある波長λ2の蛍光が検出される場合は物Bを含む等の情報が得られる。また、応用例として、樹脂サンプルに他の樹脂が混入していたり、特定の添加物が入っていると蛍光スペクトルに特徴が出るので、その結果をもって異物混入の判定等を行うことができる。このような蛍光波長の寄与度から、ユーザーが測定に有効な所望の励起光波長を複数選択する。 Next, a plurality of effective excitation light wavelengths used for determination are selected (step S3). As a result of the principal component analysis, the contribution of the fluorescence wavelength can be obtained. Specifically, when the fluorescence of a certain wavelength λ1 is detected, the substance A is included, and when the fluorescence of a certain wavelength λ2 is detected, the substance B is included. Further, as an application example, if another resin is mixed in the resin sample or a specific additive is contained, the fluorescence spectrum becomes characteristic, and the result can be used to determine the inclusion of foreign matter. From the contribution of such fluorescence wavelengths, the user selects a plurality of desired excitation light wavelengths effective for measurement.
 図1に示すフローチャートによる工程をあらかじめ行ったのち、図3に示す蛍光指紋画像取得装置を用いて蛍光指紋画像を取得する。 After performing the process according to the flowchart shown in FIG. 1 in advance, a fluorescent fingerprint image is acquired using the fluorescent fingerprint image acquisition device shown in FIG.
<蛍光指紋画像取得装置の構成>
 図3は、本発明の蛍光指紋画像取得装置の模式図である。
 図3に示すように、本発明の蛍光指紋画像取得装置100は、対象物200の蛍光指紋に基づく蛍光指紋画像を取得する装置であり、少なくとも励起光源101、測定部102、光学フィルター103、搬送機構104及び処理部105を備えている。
<Configuration of fluorescent fingerprint image acquisition device>
FIG. 3 is a schematic diagram of the fluorescent fingerprint image acquisition device of the present invention.
As shown in FIG. 3, the fluorescent fingerprint image acquisition device 100 of the present invention is a device that acquires a fluorescent fingerprint image based on the fluorescent fingerprint of the object 200, and is at least an excitation light source 101, a measuring unit 102, an optical filter 103, and a carrier. It includes a mechanism 104 and a processing unit 105.
 前記励起光源は、前記したように波長選択工程において、あらかじめ選択された複数の励起光波長の光を、対象物に照射して対象物の成分から蛍光を生じさせる。具体的には、スーパーコンティニューム光源(光ファイバーの非線形効果を利用して非常に広い波長範囲にわたって、位相の揃った強い光を出す広帯域パルス光源であり、「SC光源」とも呼ばれる。)又はLED光源等が挙げられる。これらの光源は、単独で使用してもよいし、併用してもよい。
 また、具体的な励起光波長の範囲としては、可視光の範囲内であることが好ましい。
 ここで、励起光源から照射される励起光の波長は、ユーザーが、あらかじめ選択した測定に有効な複数の励起光波長を、処理部のキーボード・マウス等を用いて入力することで、任意に変えることが可能となっている。
As described above, the excitation light source irradiates the object with light having a plurality of excitation light wavelengths selected in advance in the wavelength selection step to generate fluorescence from the components of the object. Specifically, it is a super-continue light source (a wideband pulse light source that emits strong light with a uniform phase over a very wide wavelength range by utilizing the non-linear effect of an optical fiber, and is also called an "SC light source") or an LED light source. And so on. These light sources may be used alone or in combination.
Further, the specific range of the excitation light wavelength is preferably within the range of visible light.
Here, the wavelength of the excitation light emitted from the excitation light source can be arbitrarily changed by inputting a plurality of excitation light wavelengths effective for the measurement selected in advance by the user using the keyboard, mouse, or the like of the processing unit. It is possible.
 前記搬送機構は、対象物を搬送できる機構を有していればよく、例えば、無端状のベルトをローラーで周回移動させるものや、ローラーで直接、測定物を移動させてもよいし、ローラーよりも大きな円筒状の回転ドラムの表面に沿って測定物を移動させるものであってもよい。 The transport mechanism may have a mechanism capable of transporting an object, for example, an endless belt may be moved around by a roller, a measured object may be directly moved by a roller, or a roller may be used. May also move the object to be measured along the surface of a large cylindrical rotating drum.
 前記測定部は、励起光が照射されて対象物から発光される成分特有のパターンの蛍光を受光し、受光した蛍光から励起光波長、蛍光波長及び蛍光強度を測定し、測定した蛍光指紋のデータを処理部に送信する。
 前記測定部は、一次元ラインセンサーと、当該一次元ラインセンサーの前方に設けられて、励起光より短い波長の光を吸収又は反射しカットする光学フィルターを備えている。
 一次元ラインセンサーは、例えば、複数の撮像素子が対象物の幅にわたって配置されている。そして、対象物が搬送方向に移動することで、一次元ラインセンサーは、対象物上を二次元撮像することができる。
 一次元ラインセンサーとしては、CCDセンサー(Charge Coupled Device)又はCMOSセンサー(Complementary Metal Oxide Semiconductor)などが挙げられる。一次元ラインセンサーは、各撮像素子において、光学系(レンズ)を介して対象物の表面から受光素子に入力された蛍光の光量に応じた電荷量や電圧を出力する撮像動作を行う。
 ここでは、一次元ラインセンサーは、複数の波長帯での撮像が可能であり、撮像した蛍光指紋のデータを処理部に送信する。
The measuring unit receives the fluorescence of the pattern peculiar to the component emitted from the object by being irradiated with the excitation light, measures the excitation light wavelength, the fluorescence wavelength and the fluorescence intensity from the received fluorescence, and measures the fluorescence fingerprint data. Is sent to the processing unit.
The measuring unit includes a one-dimensional line sensor and an optical filter provided in front of the one-dimensional line sensor to absorb or reflect and cut light having a wavelength shorter than that of the excitation light.
In the one-dimensional line sensor, for example, a plurality of image pickup devices are arranged over the width of an object. Then, by moving the object in the transport direction, the one-dimensional line sensor can take a two-dimensional image on the object.
Examples of the one-dimensional line sensor include a CCD sensor (Charge Coupled Device) and a CMOS sensor (Complementary Metal Oxide Semiconductor). The one-dimensional line sensor performs an image pickup operation in each image pickup device, which outputs a charge amount and a voltage according to the amount of fluorescence light input to the light receiving element from the surface of the object via an optical system (lens).
Here, the one-dimensional line sensor is capable of imaging in a plurality of wavelength bands, and transmits the captured fluorescent fingerprint data to the processing unit.
 前記光学フィルターは、励起光源の波長切り替えに同期して、当該励起光源から照射される励起光波長よりも短い波長の光を吸収又は反射しカットする所望の光学フィルターに切り替わるように制御されている。光学フィルターとしては、例えば、カットオフフィルター、バンドパスフィルター(BPF,干渉フィルター)、音響光学波長可変フィルター(AOTF)、液晶チューナブルフィルター等が挙げられる。
 なお、前記一次元ラインセンサーは、励起光源の励起光波長に対応して複数設けられていてもよく、また、光学フィルターも当該一次元ラインセンサーの前にそれぞれ設けられていてもよい。
The optical filter is controlled to switch to a desired optical filter that absorbs or reflects and cuts light having a wavelength shorter than the excitation light wavelength emitted from the excitation light source in synchronization with the wavelength switching of the excitation light source. .. Examples of the optical filter include a cutoff filter, a bandpass filter (BPF, an interference filter), an acoustic optical wavelength variable filter (AOTF), a liquid crystal tunable filter and the like.
A plurality of the one-dimensional line sensors may be provided corresponding to the excitation light wavelength of the excitation light source, and an optical filter may also be provided in front of the one-dimensional line sensor.
 前記処理部は、測定部で取得した蛍光指紋のデータから、異常検出やそれに伴う警告及び発生記録の処理を行い、蛍光指紋画像を取得する装置であり、また、蛍光指紋画像取得装置の動作全体を統括制御する。このような処理部として、例えば、一般的なパソコンなどが用いられる。なお、非常に膨大なデータ量を扱う場合や、超高速処理が必要な用途においては、専用の集積回路(ASIC:エーシック)を設けてもよい。
 処理部は、メモリ、制御部、計算処理部等を備えている。また、ユーザーがキーボード・マウスにより、処理部に測定処理条件等を入力可能となっている。
The processing unit is a device that acquires a fluorescent fingerprint image by processing abnormality detection and accompanying warnings and occurrence records from the fluorescent fingerprint data acquired by the measurement unit, and also performs the entire operation of the fluorescent fingerprint image acquisition device. Is centrally controlled. As such a processing unit, for example, a general personal computer or the like is used. A dedicated integrated circuit (ASIC: ASIC) may be provided when handling a very large amount of data or in an application requiring ultra-high-speed processing.
The processing unit includes a memory, a control unit, a calculation processing unit, and the like. In addition, the user can input measurement processing conditions and the like into the processing unit using the keyboard and mouse.
 次に、本実施形態の蛍光指紋画像取得装置における蛍光指紋画像を取得する動作について説明する。
 図4は、蛍光指紋画像取得装置で実行される処理部による制御手順を示すフローチャートである。
 図4に示すように、まず、ユーザーによって選択された測定に有効な複数の励起光波長のうち、一つ目の励起光波長を照射する励起光源に切り替える(ステップS11)。
 この励起光源の切り替えに同期して、測定部の光学フィルターも当該励起光源から照射される励起光の波長よりも短い波長の光をカットする光学フィルターに切り替える(ステップS12)。
Next, the operation of acquiring the fluorescent fingerprint image in the fluorescent fingerprint image acquisition device of the present embodiment will be described.
FIG. 4 is a flowchart showing a control procedure by the processing unit executed by the fluorescent fingerprint image acquisition device.
As shown in FIG. 4, first, among a plurality of excitation light wavelengths effective for the measurement selected by the user, the excitation light source is switched to irradiate the first excitation light wavelength (step S11).
In synchronization with the switching of the excitation light source, the optical filter of the measuring unit is also switched to an optical filter that cuts light having a wavelength shorter than the wavelength of the excitation light emitted from the excitation light source (step S12).
 次に、切り替えた励起光源から対象物に励起光を照射する(ステップS13)。励起光の照射時間や照射強度等は対象物に対応して適宜設定する。また、このとき対象物は搬送機構によって、所望の位置で照射されるように搬送されることが好ましい。
 次に、対象物に励起光を照射し、励起光波長の光によって励起された対象物からの蛍光を、測定部により受光し(ステップS14)、受光した蛍光の波長や強度を測定して、当該蛍光波長、蛍光強度及び励起光波長からなる蛍光指紋のデータを処理部に送信する。
 設定した複数の励起光波長の照射が終了していなければ(ステップS15;No)、選択した複数の励起光波長のうち、次の励起光波長を出射する励起光源に切り替える(ステップS11)。例えば、選択した複数の励起光波長がλ1とλ2の2波長である場合には、はじめに波長λ1の励起光源で照射した後、波長λ2の励起光源で照射するというサイクルを行う。
 そして、設定した複数の励起光波長の照射が全て終了したら(ステップS15;Yes)、処理部に送信された蛍光指紋のデータにより作成した蛍光指紋画像に基づき下記のような判定処理を行い、その結果を記録する(ステップS16)。
Next, the object is irradiated with the excitation light from the switched excitation light source (step S13). The irradiation time, irradiation intensity, etc. of the excitation light are appropriately set according to the object. Further, at this time, it is preferable that the object is transported so as to be irradiated at a desired position by the transport mechanism.
Next, the object is irradiated with excitation light, the fluorescence from the object excited by the light having the excitation light wavelength is received by the measuring unit (step S14), and the wavelength and intensity of the received fluorescence are measured. The fluorescence fingerprint data consisting of the fluorescence wavelength, the fluorescence intensity and the excitation light wavelength is transmitted to the processing unit.
If the irradiation of the set plurality of excitation light wavelengths is not completed (step S15; No), the light source is switched to the excitation light source that emits the next excitation light wavelength among the selected plurality of excitation light wavelengths (step S11). For example, when a plurality of selected excitation light wavelengths are two wavelengths of λ1 and λ2, a cycle of first irradiating with an excitation light source having a wavelength of λ1 and then irradiating with an excitation light source having a wavelength of λ2 is performed.
Then, when all the irradiation of the set plurality of excitation light wavelengths is completed (step S15; Yes), the following determination process is performed based on the fluorescent fingerprint image created from the fluorescent fingerprint data transmitted to the processing unit, and the determination process is performed. Record the result (step S16).
 前記判定処理は、例えば、以下の(1)~(3)に示す判定処理のパターンが挙げられるが、これらに限定されるものではない。
 (1)ある励起光波長を照射した際に、特定の蛍光波長を検出したかどうかを判定する。単純に特定の蛍光波長の有無によって判定するパターンで、あらかじめ検出したい物質固有の蛍光特性が分かっているような場合である。
 (2)ある励起光波長を照射した際に、特定の蛍光波長を検出し、かつ閾値以上の光量かどうかを判定する。例えば、特定の閾値まではOK、特定の閾値を超えたらNGとし、物質の変化(特性の変化)を判定するような場合である。このタイプの判定においては、光源や受光センサーの校正が必要となる。
 (3)励起光λ1で照射したときの蛍光λ11と、励起光λ2で照射したときのλ22の強度比率を計算し、特定の強度比率であるか否かを判定する。このタイプの判定においても、光源や受光センサーの校正が必要となる。
 上記のような各判定処理パターンは、それぞれの判定処理パターンに応じた判定テーブルを設けておき、当該判定パターンと照合することによって判定を行う。
The determination process includes, for example, the patterns of the determination process shown in the following (1) to (3), but the determination process is not limited thereto.
(1) It is determined whether or not a specific fluorescence wavelength is detected when a certain excitation light wavelength is irradiated. This is a case where the fluorescence characteristics peculiar to the substance to be detected are known in advance by a pattern simply determined by the presence or absence of a specific fluorescence wavelength.
(2) When a certain excitation light wavelength is irradiated, a specific fluorescence wavelength is detected, and it is determined whether or not the amount of light is equal to or greater than the threshold value. For example, it is OK up to a specific threshold value and NG when it exceeds a specific threshold value, and a change in a substance (change in characteristics) is determined. This type of determination requires calibration of the light source and light receiving sensor.
(3) The intensity ratio of the fluorescence λ11 when irradiated with the excitation light λ1 and the intensity ratio of λ22 when irradiated with the excitation light λ2 is calculated, and it is determined whether or not the intensity ratio is specific. This type of determination also requires calibration of the light source and light receiving sensor.
Each determination processing pattern as described above is determined by providing a determination table corresponding to each determination processing pattern and collating with the determination pattern.
 前記のような判定処理を行ったのち、異常を検出しなければ(ステップS17;No)、終了する。異常を検出した場合には(ステップS17;Yes)、表示や音声により警告を出し(ステップS18)、異常発生があった場所、時間及びどのような異常であるかなどの異常発生記録を取り(ステップS19)、処理を終了する。
 前記異常とは、例えば、蛍光の強度比率が設定範囲を超えていたり、本来蛍光を生じない箇所から蛍光が生じていた場合などが挙げられる。
After performing the determination process as described above, if no abnormality is detected (step S17; No), the process ends. When an abnormality is detected (step S17; Yes), a warning is issued by display or voice (step S18), and an abnormality occurrence record such as the place and time when the abnormality occurred and what kind of abnormality is taken is taken (step S18). Step S19), the process is terminated.
Examples of the abnormality include cases where the fluorescence intensity ratio exceeds the set range, or fluorescence is generated from a place where fluorescence is not originally generated.
 なお、本発明は上記実施形態に限定されるものではなく、様々な変更が可能である。
 上記実施形態では、あらかじめ対象物の蛍光指紋を測定しておき、測定結果から測定に有効な励起光波長を複数選択し、選択した複数の励起光波長の光を出射する励起光源より、選択した複数の励起光波長の光を波長ごとに順次発光させて、一次元ラインセンサーにより受光して、連続的に蛍光指紋画像を取得するとしたが、あらかじめ対象物の蛍光指紋を測定して有効な励起光波長を選択する工程を行わずに、単に、特定の励起光波長の光を対象物に出射し、励起された対象物からの蛍光を一次元ラインセンサーにより受光して、連続的に蛍光指紋画像を取得する構成としてもよい。
 また、上記実施形態における蛍光指紋画像取得装置では、対象物を搬送する搬送機構を設け、対象物を測定部に対して移動させる構成としたが、測定部を搬送する搬送機構を設け、測定部を対象物に対して移動させる構成としてもよい。
The present invention is not limited to the above embodiment, and various modifications can be made.
In the above embodiment, the fluorescent fingerprint of the object is measured in advance, a plurality of excitation light wavelengths effective for the measurement are selected from the measurement results, and the light is selected from the excitation light sources that emit light of the selected plurality of excitation light wavelengths. It was said that light with multiple excitation light wavelengths would be emitted sequentially for each wavelength and received by a one-dimensional line sensor to continuously acquire fluorescent fingerprint images, but effective excitation by measuring the fluorescent fingerprint of the object in advance. Without performing the step of selecting the light wavelength, the light of a specific excitation light wavelength is simply emitted to the object, the fluorescence from the excited object is received by the one-dimensional line sensor, and the fluorescence fingerprint is continuously fluorescent. It may be configured to acquire an image.
Further, in the fluorescent fingerprint image acquisition device in the above embodiment, a transport mechanism for transporting the object is provided and the object is moved with respect to the measurement unit. However, a transport mechanism for transporting the measurement unit is provided and the measurement unit is provided. May be configured to move with respect to the object.
 以上のように、本実施形態の蛍光指紋画像取得装置は、対象物の蛍光指紋に基づく蛍光指紋画像を取得する際に、あらかじめ対象物の品質に関係する有効な波長を複数選択しておき、かつ、測定部として一次元ラインセンサーを用いることで、スペクトルデータを圧縮して高速化を可能とし、かつ、簡易な構成の装置で、低コスト化を図ることができる。 As described above, in the fluorescent fingerprint image acquisition device of the present embodiment, when acquiring a fluorescent fingerprint image based on the fluorescent fingerprint of the object, a plurality of effective wavelengths related to the quality of the object are selected in advance. Moreover, by using a one-dimensional line sensor as a measuring unit, it is possible to compress spectral data to increase the speed, and it is possible to reduce the cost with a device having a simple configuration.
 不良品や端材を再利用して成型した粒状の樹脂成型品について、紫外線吸収剤を添加した樹脂成型品Aと、紫外線吸収剤を添加していない樹脂成型品Bとを準備した。
 そして、これら樹脂成型品A及びBについてそれぞれ、本発明の蛍光指紋画像取得装置を用いて、加熱前後の蛍光指紋を測定し、蛍光指紋データを主成分分析した結果を図5に示した。なお、測定時の励起光波長は250~700nmまでを10nm刻みで照射した。
 図5に示すように、主成分2と主成分3という軸によって紫外線吸収剤の有無と加熱の有無が分離できることが分かる。
 したがって、樹脂成型品の成型時において熱を何度も加えられた樹脂材料は、品質変化が生じ再生材料として利用できず、その品質変化を捉えるための指標の一つとして蛍光指紋が使えることができる。
 なお、図5に示す主成分分析の結果はデータの分布方向を係数として数値で示しているため単位は無しである。
As a granular resin molded product molded by reusing defective products and scraps, a resin molded product A to which an ultraviolet absorber was added and a resin molded product B to which an ultraviolet absorber was not added were prepared.
Then, for each of these resin molded products A and B, the fluorescent fingerprints before and after heating were measured using the fluorescent fingerprint image acquisition device of the present invention, and the results of principal component analysis of the fluorescent fingerprint data are shown in FIG. The excitation light wavelength at the time of measurement was 250 to 700 nm in 10 nm increments.
As shown in FIG. 5, it can be seen that the presence / absence of the ultraviolet absorber and the presence / absence of heating can be separated by the axes of the main component 2 and the main component 3.
Therefore, a resin material that has been repeatedly heated during molding of a resin molded product cannot be used as a recycled material due to quality changes, and fluorescent fingerprints can be used as one of the indicators for capturing the quality changes. can.
 The result of the principal component analysis shown in FIG. 5 has no unit because the distribution direction of the data is shown numerically as a coefficient.
 本発明は、対象物の蛍光指紋(蛍光特性)に基づく蛍光指紋画像を取得する際に、蛍光スペクトルデータを圧縮して高速化が可能で、かつ、装置の構成も簡略化でき、低コストが実現可能な蛍光指紋画像取得装置に利用することができる。 INDUSTRIAL APPLICABILITY According to the present invention, when acquiring a fluorescence fingerprint image based on a fluorescence fingerprint (fluorescence characteristic) of an object, the fluorescence spectrum data can be compressed to increase the speed, and the configuration of the apparatus can be simplified, resulting in low cost. It can be used in a feasible fluorescent fingerprint image acquisition device.
100 蛍光指紋画像取得装置
101 励起光源
102 測定部
103 光学フィルター
104 搬送機構
105 処理部
200 対象物
100 Fluorescent fingerprint image acquisition device 101 Excitation light source 102 Measuring unit 103 Optical filter 104 Conveying mechanism 105 Processing unit 200 Object

Claims (5)

  1.  対象物の蛍光指紋の測定に基づく蛍光指紋画像取得装置であって、
     前記対象物に、特定の励起光波長の光を出射する励起光源より照射し、
     前記励起光波長の光によって励起された前記対象物からの蛍光を、一次元ラインセンサーを有する測定部により受光し、
     前記測定部と前記対象物の相対位置を可変とし、連続的に蛍光指紋画像を取得する蛍光指紋画像取得装置。
    A fluorescent fingerprint image acquisition device based on the measurement of the fluorescent fingerprint of an object.
    The object is irradiated with an excitation light source that emits light having a specific excitation light wavelength.
    The fluorescence from the object excited by the light of the excitation light wavelength is received by a measuring unit having a one-dimensional line sensor, and the fluorescence is received.
    A fluorescent fingerprint image acquisition device that continuously acquires a fluorescent fingerprint image by making the relative positions of the measuring unit and the object variable.
  2.  あらかじめ前記対象物の前記蛍光指紋として、励起光波長、蛍光波長及び蛍光強度を測定しておき、測定に有効な励起光波長を複数選択し、
     選択した複数の前記励起光波長の光を出射する励起光源より、前記選択した複数の前記励起光波長の光を波長ごとに順次発光させ、前記対象物に照射し、
     各前記励起光波長の光によって励起された前記対象物からの蛍光を、前記測定部により受光する請求項1に記載の蛍光指紋画像取得装置。
    The excitation light wavelength, the fluorescence wavelength, and the fluorescence intensity are measured in advance as the fluorescence fingerprint of the object, and a plurality of excitation light wavelengths effective for the measurement are selected.
    From the excitation light source that emits the light of the plurality of selected excitation light wavelengths, the light of the plurality of selected excitation light wavelengths is sequentially emitted for each wavelength, and the object is irradiated with the light.
    The fluorescent fingerprint image acquisition device according to claim 1, wherein the measuring unit receives fluorescence from the object excited by light having each excitation light wavelength.
  3.  前記励起光源が、スーパーコンティニューム光源又はLEDである請求項1又は請求項2に記載の蛍光指紋画像取得装置。 The fluorescent fingerprint image acquisition device according to claim 1 or 2, wherein the excitation light source is a super-continue light source or an LED.
  4.  前記一次元ラインセンサーの前に、励起光より短い波長の光を吸収又は反射しカットする光学フィルターを備え、
     前記励起光源の波長切り替えに同期して前記光学フィルターを切り替える請求項1から請求項3までのいずれか一項に記載の蛍光指紋画像取得装置。
    In front of the one-dimensional line sensor, an optical filter that absorbs or reflects light having a wavelength shorter than that of the excitation light and cuts the light is provided.
    The fluorescent fingerprint image acquisition device according to any one of claims 1 to 3, wherein the optical filter is switched in synchronization with the wavelength switching of the excitation light source.
  5.  前記励起光源の前記励起光波長に対応して複数の前記一次元ラインセンサーを備え、
     各当該一次元ラインセンサーの前に、それぞれ前記励起光波長より短い波長の光を吸収又は反射しカットする光学フィルターを備え、
     当該一次元ラインセンサーを前記励起光源の切り替えに同期させて受光する請求項1から請求項4までのいずれか一項に記載の蛍光指紋画像取得装置。
    A plurality of the one-dimensional line sensors corresponding to the excitation light wavelength of the excitation light source are provided.
    In front of each one-dimensional line sensor, an optical filter that absorbs or reflects light having a wavelength shorter than the excitation light wavelength and cuts the light is provided.
    The fluorescent fingerprint image acquisition device according to any one of claims 1 to 4, wherein the one-dimensional line sensor receives light in synchronization with the switching of the excitation light source.
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JP2013108855A (en) * 2011-11-21 2013-06-06 National Agriculture & Food Research Organization Discrimination filter design method, discrimination method, discrimination filter set, discrimination device and program
JP2015130864A (en) * 2009-05-15 2015-07-23 ビオメリュー・インコーポレイテッド System and methods for rapid identification and/or characterization of microbial agent in sample
JP2018136151A (en) * 2017-02-20 2018-08-30 日清オイリオグループ株式会社 Determination method and production method for oil and fat composition

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05215688A (en) * 1991-07-10 1993-08-24 Asahi Glass Co Ltd Method and apparatus for detecting scatterer in light pervious body
JP2015130864A (en) * 2009-05-15 2015-07-23 ビオメリュー・インコーポレイテッド System and methods for rapid identification and/or characterization of microbial agent in sample
JP2013108855A (en) * 2011-11-21 2013-06-06 National Agriculture & Food Research Organization Discrimination filter design method, discrimination method, discrimination filter set, discrimination device and program
JP2018136151A (en) * 2017-02-20 2018-08-30 日清オイリオグループ株式会社 Determination method and production method for oil and fat composition

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