WO2021214360A1 - Uso de ésteres de (z)-3-hexenilo y método para proteger plantas frente a plagas - Google Patents
Uso de ésteres de (z)-3-hexenilo y método para proteger plantas frente a plagas Download PDFInfo
- Publication number
- WO2021214360A1 WO2021214360A1 PCT/ES2021/070259 ES2021070259W WO2021214360A1 WO 2021214360 A1 WO2021214360 A1 WO 2021214360A1 ES 2021070259 W ES2021070259 W ES 2021070259W WO 2021214360 A1 WO2021214360 A1 WO 2021214360A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- spp
- plants
- hymenoptera
- formula
- compound
- Prior art date
Links
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 74
- 238000000034 method Methods 0.000 title claims abstract description 37
- -1 (z)-3-hexenyl esters Chemical class 0.000 title abstract description 10
- 150000001875 compounds Chemical class 0.000 claims abstract description 41
- 239000000203 mixture Substances 0.000 claims abstract description 18
- 230000008260 defense mechanism Effects 0.000 claims abstract description 9
- 230000004936 stimulating effect Effects 0.000 claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims description 166
- LGTLDEUQCOJGFP-WAYWQWQTSA-N cis-3-Hexenyl propanoate Chemical compound CC\C=C/CCOC(=O)CC LGTLDEUQCOJGFP-WAYWQWQTSA-N 0.000 claims description 31
- 241000258937 Hemiptera Species 0.000 claims description 30
- 241000257303 Hymenoptera Species 0.000 claims description 26
- 241001454293 Tetranychus urticae Species 0.000 claims description 19
- 230000007123 defense Effects 0.000 claims description 18
- 241000255777 Lepidoptera Species 0.000 claims description 16
- 241001414989 Thysanoptera Species 0.000 claims description 15
- 235000020971 citrus fruits Nutrition 0.000 claims description 15
- 241000238876 Acari Species 0.000 claims description 13
- 241000207199 Citrus Species 0.000 claims description 13
- 241000254127 Bemisia tabaci Species 0.000 claims description 12
- 241000894007 species Species 0.000 claims description 12
- XBDQKXXYIPTUBI-UHFFFAOYSA-N Propionic acid Chemical compound CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims description 11
- 230000007246 mechanism Effects 0.000 claims description 10
- YYJNOYZRYGDPNH-MFKUBSTISA-N fenpyroximate Chemical compound C=1C=C(C(=O)OC(C)(C)C)C=CC=1CO/N=C/C=1C(C)=NN(C)C=1OC1=CC=CC=C1 YYJNOYZRYGDPNH-MFKUBSTISA-N 0.000 claims description 9
- 241000488583 Panonychus ulmi Species 0.000 claims description 8
- 241001454295 Tetranychidae Species 0.000 claims description 8
- 230000037125 natural defense Effects 0.000 claims description 8
- 241000189579 Thripidae Species 0.000 claims description 7
- 241000424578 Anagyrus Species 0.000 claims description 4
- 241001547764 Aphelinidae Species 0.000 claims description 4
- 241001523597 Aphidius Species 0.000 claims description 4
- 241001454985 Aphytis Species 0.000 claims description 4
- 241000254123 Bemisia Species 0.000 claims description 4
- 241000256816 Braconidae Species 0.000 claims description 4
- 241001546529 Diglyphus Species 0.000 claims description 4
- 241001455007 Encarsia Species 0.000 claims description 4
- 241001147399 Encyrtidae Species 0.000 claims description 4
- 241001300499 Eretmocerus Species 0.000 claims description 4
- 241001541327 Eulophidae Species 0.000 claims description 4
- 241000725028 Kuwayama Species 0.000 claims description 4
- 241001366808 Liviidae Species 0.000 claims description 4
- 241000545572 Lysiphlebus Species 0.000 claims description 4
- 241000422669 Metaphycus Species 0.000 claims description 4
- 241001454656 Scelionidae Species 0.000 claims description 4
- 241000000514 Tamarixia Species 0.000 claims description 4
- 241001519528 Telenomus Species 0.000 claims description 4
- 241000489254 Tetranychus takafujii Species 0.000 claims description 4
- 241001389010 Tuta Species 0.000 claims description 4
- 241000724057 Chaetanaphothrips orchidii Species 0.000 claims description 3
- 241001546544 Cirrospilus Species 0.000 claims description 3
- 241001034432 Eutetranychus banksi Species 0.000 claims description 3
- 241000851181 Eutetranychus orientalis Species 0.000 claims description 3
- 241000925468 Paraleyrodes minei Species 0.000 claims description 3
- 241001414857 Psyllidae Species 0.000 claims description 3
- 241000256247 Spodoptera exigua Species 0.000 claims description 3
- 241001454294 Tetranychus Species 0.000 claims description 3
- 241000256618 Trichogramma Species 0.000 claims description 3
- 241000462092 Trioza erytreae Species 0.000 claims description 3
- 238000009792 diffusion process Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 241000488581 Panonychus citri Species 0.000 claims description 2
- 241000256680 Trichogrammatidae Species 0.000 claims description 2
- 241001454604 Trissolcus Species 0.000 claims description 2
- 239000000443 aerosol Substances 0.000 claims description 2
- 241000422252 Cales Species 0.000 claims 1
- 241000108082 Dialeurodes Species 0.000 claims 1
- 241000282326 Felis catus Species 0.000 claims 1
- 241000410838 Gracillariidae Species 0.000 claims 1
- 241000256259 Noctuidae Species 0.000 claims 1
- 241000488585 Panonychus Species 0.000 claims 1
- 241000910058 Pezothrips Species 0.000 claims 1
- 241000910062 Pezothrips kellyanus Species 0.000 claims 1
- 239000006199 nebulizer Substances 0.000 claims 1
- 150000002148 esters Chemical class 0.000 abstract description 5
- ZCHOPXVYTWUHDS-WAYWQWQTSA-N cis-3-hexenyl butyrate Chemical compound CCCC(=O)OCC\C=C/CC ZCHOPXVYTWUHDS-WAYWQWQTSA-N 0.000 abstract description 4
- ZCHOPXVYTWUHDS-UHFFFAOYSA-N cis-3-hexenyl n-butyrate Natural products CCCC(=O)OCCC=CCC ZCHOPXVYTWUHDS-UHFFFAOYSA-N 0.000 abstract description 3
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 abstract description 2
- 241000227653 Lycopersicon Species 0.000 description 57
- 238000011282 treatment Methods 0.000 description 39
- ZNJFBWYDHIGLCU-HWKXXFMVSA-N jasmonic acid Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(O)=O)CCC1=O ZNJFBWYDHIGLCU-HWKXXFMVSA-N 0.000 description 36
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 26
- 239000003039 volatile agent Substances 0.000 description 25
- 230000004044 response Effects 0.000 description 23
- 108090000623 proteins and genes Proteins 0.000 description 20
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 18
- ZNJFBWYDHIGLCU-UHFFFAOYSA-N jasmonic acid Natural products CCC=CCC1C(CC(O)=O)CCC1=O ZNJFBWYDHIGLCU-UHFFFAOYSA-N 0.000 description 18
- 238000012360 testing method Methods 0.000 description 18
- 230000004913 activation Effects 0.000 description 17
- 238000002474 experimental method Methods 0.000 description 17
- 241001090158 Nesidiocoris tenuis Species 0.000 description 15
- 229920001684 low density polyethylene Polymers 0.000 description 15
- 239000004702 low-density polyethylene Substances 0.000 description 15
- 230000000694 effects Effects 0.000 description 13
- 230000014509 gene expression Effects 0.000 description 12
- 244000062645 predators Species 0.000 description 12
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 12
- 230000002441 reversible effect Effects 0.000 description 11
- 235000002566 Capsicum Nutrition 0.000 description 9
- 235000013601 eggs Nutrition 0.000 description 9
- 230000007613 environmental effect Effects 0.000 description 9
- 239000003550 marker Substances 0.000 description 9
- 240000007533 Cratoxylum formosum Species 0.000 description 8
- 241000526125 Diaphorina citri Species 0.000 description 8
- 241000927584 Frankliniella occidentalis Species 0.000 description 8
- 240000003889 Piper guineense Species 0.000 description 8
- 229920001903 high density polyethylene Polymers 0.000 description 8
- 239000004700 high-density polyethylene Substances 0.000 description 8
- 229920003023 plastic Polymers 0.000 description 8
- 239000004033 plastic Substances 0.000 description 8
- 230000019491 signal transduction Effects 0.000 description 8
- 230000037361 pathway Effects 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 description 6
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 6
- 239000000575 pesticide Substances 0.000 description 6
- 238000011002 quantification Methods 0.000 description 6
- 229960004889 salicylic acid Drugs 0.000 description 6
- 241000101065 Macrolophus pygmaeus Species 0.000 description 5
- 239000006002 Pepper Substances 0.000 description 5
- 235000016761 Piper aduncum Nutrition 0.000 description 5
- 235000017804 Piper guineense Nutrition 0.000 description 5
- 235000008184 Piper nigrum Nutrition 0.000 description 5
- 244000038280 herbivores Species 0.000 description 5
- 238000007726 management method Methods 0.000 description 5
- 230000002103 transcriptional effect Effects 0.000 description 5
- 230000003442 weekly effect Effects 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 240000004160 Capsicum annuum Species 0.000 description 4
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 4
- 241001454772 Encarsia formosa Species 0.000 description 4
- 108700039887 Essential Genes Proteins 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 4
- 241000758706 Piperaceae Species 0.000 description 4
- 241001466030 Psylloidea Species 0.000 description 4
- 240000006909 Tilia x europaea Species 0.000 description 4
- 235000011941 Tilia x europaea Nutrition 0.000 description 4
- 238000000540 analysis of variance Methods 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 239000004571 lime Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000002243 precursor Substances 0.000 description 4
- 239000005871 repellent Substances 0.000 description 4
- 230000002940 repellent Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 3
- 241000254124 Aleyrodidae Species 0.000 description 3
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 3
- 241001672694 Citrus reticulata Species 0.000 description 3
- 241001512104 Citrus sinensis x Citrus trifoliata Species 0.000 description 3
- 241001454728 Gelechiidae Species 0.000 description 3
- 101100128612 Glycine max LOX1.2 gene Proteins 0.000 description 3
- 101150046318 LOX2 gene Proteins 0.000 description 3
- 241001414825 Miridae Species 0.000 description 3
- 101100343701 Mus musculus Loxl1 gene Proteins 0.000 description 3
- 101150091997 PIN2 gene Proteins 0.000 description 3
- 108010033711 Telomeric Repeat Binding Protein 1 Proteins 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- RRGOKSYVAZDNKR-ARJAWSKDSA-M cis-3-hexenylacetate Chemical compound CC\C=C/CCCC([O-])=O RRGOKSYVAZDNKR-ARJAWSKDSA-M 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 238000003973 irrigation Methods 0.000 description 3
- 230000002262 irrigation Effects 0.000 description 3
- 235000017932 juta Nutrition 0.000 description 3
- 235000021374 legumes Nutrition 0.000 description 3
- 230000037353 metabolic pathway Effects 0.000 description 3
- 229960001047 methyl salicylate Drugs 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- NPFVOOAXDOBMCE-UHFFFAOYSA-N trans-3-hexenyl acetate Natural products CCC=CCCOC(C)=O NPFVOOAXDOBMCE-UHFFFAOYSA-N 0.000 description 3
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 2
- 235000005254 Allium ampeloprasum Nutrition 0.000 description 2
- 240000006108 Allium ampeloprasum Species 0.000 description 2
- 244000291564 Allium cepa Species 0.000 description 2
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 2
- 241000353216 Aphelinus Species 0.000 description 2
- 241000239290 Araneae Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102100039339 Atrial natriuretic peptide receptor 1 Human genes 0.000 description 2
- 235000002567 Capsicum annuum Nutrition 0.000 description 2
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 2
- 235000021538 Chard Nutrition 0.000 description 2
- 235000005979 Citrus limon Nutrition 0.000 description 2
- 244000131522 Citrus pyriformis Species 0.000 description 2
- 240000000560 Citrus x paradisi Species 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000009849 Cucumis sativus Nutrition 0.000 description 2
- 235000009854 Cucurbita moschata Nutrition 0.000 description 2
- 240000001980 Cucurbita pepo Species 0.000 description 2
- 235000009852 Cucurbita pepo Nutrition 0.000 description 2
- 241000219130 Cucurbita pepo subsp. pepo Species 0.000 description 2
- 235000003954 Cucurbita pepo var melopepo Nutrition 0.000 description 2
- 241000526124 Diaphorina Species 0.000 description 2
- 241000285023 Formosa Species 0.000 description 2
- 241000189565 Frankliniella Species 0.000 description 2
- 101000961044 Homo sapiens Atrial natriuretic peptide receptor 1 Proteins 0.000 description 2
- 241001325860 Lacanobia oleracea Species 0.000 description 2
- 240000008415 Lactuca sativa Species 0.000 description 2
- 235000003228 Lactuca sativa Nutrition 0.000 description 2
- 241000158764 Murraya Species 0.000 description 2
- 206010035148 Plague Diseases 0.000 description 2
- 244000061458 Solanum melongena Species 0.000 description 2
- 238000000692 Student's t-test Methods 0.000 description 2
- 241000845423 Tamarixia radiata Species 0.000 description 2
- 102100036497 Telomeric repeat-binding factor 1 Human genes 0.000 description 2
- 241000339374 Thrips tabaci Species 0.000 description 2
- 230000006399 behavior Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- DSSYKIVIOFKYAU-UHFFFAOYSA-N camphor Chemical compound C1CC2(C)C(=O)CC1C2(C)C DSSYKIVIOFKYAU-UHFFFAOYSA-N 0.000 description 2
- 239000001511 capsicum annuum Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- GXANMBISFKBPEX-ARJAWSKDSA-N cis-3-hexenal Chemical compound CC\C=C/CC=O GXANMBISFKBPEX-ARJAWSKDSA-N 0.000 description 2
- 238000009109 curative therapy Methods 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000005712 elicitor Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000002480 mineral oil Substances 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- YLYBTZIQSIBWLI-UHFFFAOYSA-N octyl acetate Chemical compound CCCCCCCCOC(C)=O YLYBTZIQSIBWLI-UHFFFAOYSA-N 0.000 description 2
- 238000009329 organic farming Methods 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 235000020354 squash Nutrition 0.000 description 2
- 238000012353 t test Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 230000001960 triggered effect Effects 0.000 description 2
- UFLHIIWVXFIJGU-ARJAWSKDSA-N (Z)-hex-3-en-1-ol Chemical compound CC\C=C/CCO UFLHIIWVXFIJGU-ARJAWSKDSA-N 0.000 description 1
- 239000000267 (Z)-hex-3-en-1-ol Substances 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- FVFVNNKYKYZTJU-UHFFFAOYSA-N 6-chloro-1,3,5-triazine-2,4-diamine Chemical compound NC1=NC(N)=NC(Cl)=N1 FVFVNNKYKYZTJU-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 206010001488 Aggression Diseases 0.000 description 1
- 241001664794 Amblyseius swirskii Species 0.000 description 1
- 241001635552 Anthocoridae Species 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 241000132092 Aster Species 0.000 description 1
- 235000005976 Citrus sinensis Nutrition 0.000 description 1
- 240000002319 Citrus sinensis Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 241000409837 Dentex maroccanus Species 0.000 description 1
- 241001205778 Dialeurodes citri Species 0.000 description 1
- 241000678169 Dicyphus Species 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- 229920002907 Guar gum Polymers 0.000 description 1
- 101000651211 Homo sapiens Transcription factor PU.1 Proteins 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 241000500891 Insecta Species 0.000 description 1
- 241001313288 Labia Species 0.000 description 1
- 229920000161 Locust bean gum Polymers 0.000 description 1
- 235000002262 Lycopersicon Nutrition 0.000 description 1
- VKEQBMCRQDSRET-UHFFFAOYSA-N Methylone Chemical compound CNC(C)C(=O)C1=CC=C2OCOC2=C1 VKEQBMCRQDSRET-UHFFFAOYSA-N 0.000 description 1
- 241001421711 Mithras Species 0.000 description 1
- 101150072410 NPR5 gene Proteins 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241001635530 Orius laevigatus Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 241001525654 Phyllocnistis citrella Species 0.000 description 1
- 241001481699 Phytoseiidae Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 244000202052 Poncirus trifoliata Species 0.000 description 1
- 235000000404 Poncirus trifoliata Nutrition 0.000 description 1
- 241000384969 Priva Species 0.000 description 1
- 241001646398 Pseudomonas chlororaphis Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 101000836070 Rattus norvegicus Serine protease inhibitor A3L Proteins 0.000 description 1
- 101710142113 Serine protease inhibitor A3K Proteins 0.000 description 1
- 235000002634 Solanum Nutrition 0.000 description 1
- 241000207763 Solanum Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 235000002560 Solanum lycopersicum Nutrition 0.000 description 1
- 244000208778 Thrinax radiata Species 0.000 description 1
- 241000016010 Tomato spotted wilt orthotospovirus Species 0.000 description 1
- 102100027654 Transcription factor PU.1 Human genes 0.000 description 1
- 241001389006 Tuta absoluta Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 230000016571 aggressive behavior Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000001887 anti-feedant effect Effects 0.000 description 1
- 229940019748 antifibrinolytic proteinase inhibitors Drugs 0.000 description 1
- 239000005667 attractant Substances 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 230000004790 biotic stress Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011449 brick Substances 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 230000031902 chemoattractant activity Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 229960005188 collagen Drugs 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000011217 control strategy Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 229940099112 cornstarch Drugs 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 239000000295 fuel oil Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 229940014259 gelatin Drugs 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000000665 guar gum Substances 0.000 description 1
- 235000010417 guar gum Nutrition 0.000 description 1
- 229960002154 guar gum Drugs 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- UFLHIIWVXFIJGU-UHFFFAOYSA-N hex-3-en-1-ol Natural products CCC=CCCO UFLHIIWVXFIJGU-UHFFFAOYSA-N 0.000 description 1
- GXANMBISFKBPEX-UHFFFAOYSA-N hex-3c-enal Natural products CCC=CCC=O GXANMBISFKBPEX-UHFFFAOYSA-N 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 230000009001 hormonal pathway Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004920 integrated pest control Methods 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 235000010420 locust bean gum Nutrition 0.000 description 1
- 239000000711 locust bean gum Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 201000002266 mite infestation Diseases 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000010690 paraffinic oil Substances 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 230000003032 phytopathogenic effect Effects 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 238000013138 pruning Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 239000006273 synthetic pesticide Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
- A01N25/06—Aerosols
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P15/00—Biocides for specific purposes not provided for in groups A01P1/00 - A01P13/00
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C69/00—Esters of carboxylic acids; Esters of carbonic or haloformic acids
- C07C69/003—Esters of saturated alcohols having the esterified hydroxy group bound to an acyclic carbon atom
Definitions
- the present invention relates to the use of (Z) -3-bexenyl esters (acetate, propanoate or butanoate), or of a composition comprising said compounds, to protect plants, in particular agricultural crops, against pests, by means of the stimulation of the defense mechanisms that the plants themselves have.
- the invention also relates to a method for protecting plants against pests by applying said esters to plants.
- the present invention falls within the technical field of plant pest control.
- the first line of crop protection should be the implementation of preventive control methods such as cultural methods, the use of resistant / tolerant plants, the application of strict quarantine regulations, and the promotion of natural pest control. It is in this foregoing context that the use of the present invention would be framed.
- biological pest control should be the first option to use, if necessary in combination with other control tactics. GIF (where pesticides would be last).
- Tomato plants can have different degrees of attraction for pests and natural enemies depending on whether the phytophagy is produced, for example, by N. tenuis, Macrolophus pygmaeus (Rambur) or Dicyphus maroccanus Wagner (Hemiptera: Miridae).
- N. tenuis Macrolophus pygmaeus
- Dicyphus maroccanus Wagner Hemiptera: Miridae
- Iaevigatus triggers the release of a mixture of volatiles (5 terpenes, 2 GLVs, methyl salicylate and one to be identified) and the activation of the metabolic pathways of jasmonic acid and salicylic acid.
- the phytophagy of the myrids N. tenuis and M. pygmaeus in pepper also caused repellency for B. tabaci and F. occidentalis and attraction for E, formosa and triggered volatiles of a nature similar to those triggered by O. / aevigatus,
- (Z) -3-hexenyl esters are elicitors of the induction of direct and indirect defenses in crops, such as tomato cultivation. Specifically, it has been possible to activate plants defensively simply by exposing them to these synthetic volatiles.
- the exposure of the esters of (Z) -3-hexenyl have been described as repellants of phytophagous plagues the whitefly, Bemisia tabaci and attractant of natural enemies such as the whitefly parasitoid, Encarsia formosa (Pérez-Hedo, M. et ai. Biocontroi, 63: 203-213 (2016)). Attraction to parasitoids is an indirect effect that can benefit the biological control of a pest.
- the present invention is a sustainable and biorational pest control tool based on communication between plants for crop protection.
- the plants use the emission of these volatiles to communicate certain aggressions to their “neighbors”.
- the plants receiving these signals enter a state of alert in which they prepare for a future attack. This is known as priming.
- the plant is defensively activated through the hormonal pathways of jasmonic acid and salicylic acid, both phytohormones involved in defense mechanisms in the plant.
- the present invention refers to the use of at least one compound of formula I defined above, or of a composition comprising it, for the protection of plants against pests by stimulating the natural defense mechanisms of these plants.
- the compounds used in the present invention are (Z) -3-hexenyl acetate, propanoate or butanoate.
- the invention relates to the use of (Z) -3-hexenyl propanoate, or of a composition comprising it, for the protection of plants against pests by stimulating the natural defense mechanisms of said plants. .
- the defense mechanisms of plants cause the repellency of at least one pest selected from the list comprising: Bemisia tabaci (Gennadius) and Triaieurodes vaporariorum (Westwood), Aieurothrixus floccosus (Maskell), Dialeurodes citri (Ashmead) and Paraleyrodes minei laccarino (Hemiptera: Aieyroididae), Frankliniella ocddentalis Pergande thrips, Pezothr ⁇ ps kellyanus Bagnall, Chaetanaphothrips orchidii (Moulton) and Thrips tabaci Lindeman (Thysanoptera: Thripidae), eastern red spiders, Tetraisutechus urtchus (Kleeutinchus Eutetranychus banksi (McGregor), T.
- Bemisia tabaci Genenadius
- the pest is selected from the list comprising: the whitefly Bemisia tabad (Gennadius) (Hemiptera: Aleyrodidae), the trips Frankliniella occidentalis Pergande (Thysanoptera: Thripidae), the red spider Tetranychus urticae Koch (Acari : Tetranychidae), the lepidopteran Tula absolute (Meyrick) (Lepidoptera: Gelechiidae) and the psilid Diaphorina citri.
- the whitefly Bemisia tabad (Gennadius) (Hemiptera: Aleyrodidae)
- the trips Frankliniella occidentalis Pergande Thysanoptera: Thripidae
- the red spider Tetranychus urticae Koch Acari : Tetranychidae
- the lepidopteran Tula absolute Meyrick
- Lepidoptera Gelechiidae
- the defense mechanisms of plants cause the attraction of at least one parasitoid selected from the list comprising: species of the genera Encarsia spp., Aphytis spp. Limes spp., Eretmocerus spp., Aphelinus spp., (Hymenoptera: Aphelinidae), Aphidius spp. Lysiphlebus spp. (Hymenoptera: Braconidae) Metaphycus spp., Anagyrus spp. (Hymenoptera: Encyrtidae), Tamarixia spp. Citrostichus spp., Cirrospilus spp., Diglyphus spp.
- Hymenoptera Eulophidae
- Trissolcus spp. Telenomus spp.
- Telenomus spp. Hymenoptera: Scelionidae
- Trichogramma spp Hymenoptera. Trichogrammatidae
- the aforementioned compound has been shown to repel pests of horticultural crops of different orders of Insecta (Hemiptera, Thysanoptera and Lepidoptera), which was not to be expected precisely because they are very phylogenetically separated species. In the case of citrus fruits, it has been observed how activation with this same volatile reduces the spawning of the psyid Diaphor ⁇ na citri.
- the protected plants are horticultural plants or citrus plants.
- Horticultural plants are preferably selected from the list comprising: tomatoes, peppers, aubergines, onions, leeks, squash, zucchini, cucumbers, chard, lettuce and legumes.
- the horticultural plants are tomato (Solanum iycopersicum) or bell pepper (Capsicum annuum) plants.
- Citrus fruits are preferably selected from the list comprising lemons, limes, oranges, grapefruits and mandarins.
- the plant is a tomato plant and the pest is the whitefly B, tabaci, the trips Frankliniella occidentalis or the lepidopteran Juta Absolute.
- the plant is a citrus fruit and the pest is the psilid Diaphorina citri.
- the use comprises the application of the compounds according to Formula I by means of diffusers, which makes it possible to efficiently use said compounds via exposure under field conditions as an inducer / elicitor of plant defenses.
- This system of emission through diffusers achieves that, in the atmosphere that surrounds the plants to be protected, a sustained and sufficient concentration of the inducer is obtained, which would not be obtained with a specific application of the same.
- “diffuser” also called “emitter” is understood to be a device or vehicle (formulation) that distributes at least one of the compounds of the invention (Formula i) via exposure thereof at a controlled rate. to plants under field conditions.
- the devices are containers for the compounds according to Formula I that allow said substances to be released into the atmosphere in a controlled manner through a membrane, a valve or even through the wall of the container itself.
- Vehicles are formulations of liquid, gelled or pasty substances that contain the compounds according to Formula I and that are applied in the form of drops or granules so that the volatiles are emitted as the vehicle degrades or dehydrates.
- formulations or vehicles can be applied on some part of the plant, on the substrate, on structures in the crop or anywhere from which it can be emitted close to the plants.
- substances such as paraffins, silicones, pectins, heavy oils, polysaccharides, proteins, etc.
- the following can be named by way of example: agar-agar, alginine, carrageenan, collagen, cornstarch, gelatin, guar gum, locust bean gum, pectin, xanthan gum, mineral oil, silicone oil, paraffins, definas or mineral or vegetable oils.
- Diffusers are preferably devices of two types:
- passive emitters that consist of a container of the compounds according to Formula I configured to allow the diffusion of said substances, so the container must be permeable to the compounds of Formula I, either through their own enclosure of a polymeric material or through a membrane placed in part of its enclosure to regulate the emission;
- the compounds according to Formula i are applied by means of diffusers consisting of low density polyethylene (LDPE) vials.
- LDPE low density polyethylene
- the present invention refers to a method for the protection of plants against pests by stimulating the natural defense mechanisms of said plants, said method is characterized in that it comprises contacting at least one compound of Formula i , or a composition comprising it, with plants.
- the contact can be direct, through the direct application of the compound to the plant or indirect, through the exposure of the plant to an environment that contains said compound.
- the pest is selected from the list comprising: Bemisia tabaci (Gennadius) and Tnaleurodes vaporariorum (Westwood), Aieurothr ⁇ xus f ⁇ occosus (Maskell), Diaieurodes citri (Ashmead) and Parateyrodes minei laccarino (Hemiptera: Aleyroididae) the trips Frankliniella occidenialis Pergande, Pezothr ⁇ ps kellyanus Bagnall, Chaetanaphothrips orchidii (Moulton) and Thrips tabaci Lindeman (Thysanoptera: Thripidae), the red spiders Tetranychus urticae, Eutetranychus orientalis (Klein), Eutetranyorchus, Panregony 7 ) Koch and Tetranychus evansi Baker & Pritchard (Acari: Tetranychida
- the pest is selected from the list comprising: the whitefly Bemisia ⁇ abaci (Gennadius) (Hemiptera: Aleyrodidae), the thrips Frankiiniella occidentalis Pergande (Thysanoptera: Thripidae), the red spider Tetranychus ur ⁇ icae Koch (Acari : Tetranychidae), the lepidoptera Juta absolu (Meyrick) (Lepidoptera: Gelechiidae) and the psyllid Diaphor ⁇ na citri.
- plant protection occurs by attracting parasitoids selected from the list comprising: species of the genera Encarsia spp, Aphytis spp. Limes spp ,, Eretmocerus spp., Aphelinus spp., (Hymenoptera: Aphelinidae), Aphidius spp. Lysiphlebus spp. (Hymenoptera: Braconidae) Metaphycus spp., Anagyrus spp. (Hymenoptera: Encyrtidae), Tamarixia spp. Citrostichus spp., Cirrospilus spp., Diglyphus spp.
- Tr ⁇ ssolcus spp. Telenomus spp.
- Tr ⁇ chogramma spp. Tr ⁇ chogramma spp.
- the protected plants are horticultural plants or citrus plants.
- Horticultural plants are preferably selected from the list comprising: tomatoes, peppers, aubergines, onions, leeks, squash, zucchini, cucumbers, chard, lettuce and legumes.
- the horticultural plants are tomato (Solanum lycopersicum) or bell pepper (Capsicum annuum) plants.
- Citrus fruits are preferably selected from the list comprising lemons, limes, oranges, grapefruits and mandarins.
- the plant is a tomato plant and the pest is the whitefly B. tabaci, the thrips Frankiiniella occidentalis or the lepidopteran Juta absolu.
- the plant is a citrus fruit and the pest is the psyllid Diaphor ⁇ na citr ⁇ .
- the compounds according to Formula I, or of a composition comprising them are applied through the use of diffusers.
- Diffusers and preferred embodiments thereof according to the present invention have been defined in the first aspect of the invention.
- the compounds according to Formula i are applied by means of consistent diffusers in low-density polyethylene vials.
- the application is carried out at the rate of one diffuser for every 20 m 2 of planted area.
- the diffuser distributes the compound to the environment that surrounds the plants and they detect it.
- the compounds according to Formula I are applied to the culture environment at a dose between 25 mg / ha / day and 25 g / ha / day, and more preferably between 200 mg / ha / day and 10 g / ha / day.
- the present invention is a new pest management method not used to date in any crop.
- Fig, 1 Franscriptional response of the defensive genes PR1 (a marker gene for the salicylic acid signaling pathway, SA) (Fig. 1A) and SI-PI-I and Sl-PI-ll (two markers for inhibitors of plant proteinases) (fig, 1B and 1C respectively) in tomato plants exposed to (Z) -3-hexenyl acetate (HA), (Z) -3-hexenyl butanoate (HB) and (Z) propanoate -3-bexenyl (HP) and the control.
- Fig. 2 Transcriptional response of the PIN2 gene (a marker gene for the jasmonic acid (JA) signaling pathway in tomato plants exposed under semi-field conditions to (Z) -3-hexenyl propanoate [(Z) -3-HP] with different volatile emission levels: 5 mg / day / diffuser in the low-density polyethylene (LD) emitter and 0.01 mg / day / diffuser in the high-density polyethylene (HD) emitter.
- the control treatment consists of in plants not exposed to the volatile Significant differences based on the ANOVA test are marked with different letters (P ⁇ 0.05).
- Fig. 3 Transcriptional response of the defense genes PR1 (a marker gene for the SA signaling pathway) (Fig. 3A) and PIN2 (a marker gene for the jasmonic acid signaling pathway) (Fig. 3B) in plants tomatoes exposed under field conditions to (Z) -3-hexeni! or [(Z) -3-HP] propanoate released in a polymeric (LD) dispenser 4 ml low-density polyethylene vial (KarieM) one day before, 4 and 8 weeks after the placement of the diffuser. The significant differences between treatments for each of the dates are marked with an asterisk (f-test; P ⁇ 0.05). Number of Nesid ⁇ coris tenuis per plant (mean ⁇ standard error) (fig.
- Fig. 3C and number of infested leaflets per plant (mean ⁇ standard error) (fig. 3D) in the commercial greenhouse (GLMM, P ⁇ 0.05).
- Fig. 5 Number of receptive shoots (mean ⁇ SE) and clutch (mean ⁇ SE) of Diaphorina streak the Murraya panicuiata seedlings used as sentinel plants with and without a diffuser loaded with HP. Different letters on the bars show significant differences (t-test P ⁇ 0.05).
- Example 1 Defensive activation tests in tomato via exposure under laboratory conditions
- the nucleotides of the EF1 housekeeping gene were forward 5'-GATTGGT GGT ATT GGAACT GT C-3 '(SEQ ID NO: 7) and reverse: 50-AGCTT CGT GGTGCATCT C-3Q (SEQ ID NO: 8).
- (Z) -3-hexenyl propanoate was selected from among the three volatiles as a model for (Z) -3-hexenyl asters.
- urticae were obtained from a colony established in the IVIA in 2011 whose original origin was clementines located in La Plana (Castelló, Spain). The mites were kept on tomato plants in a climatic chamber under the same conditions described above.
- Females of T. absolut were obtained from colonies on tomato kept in a greenhouse at the IVIA at 25 ⁇ 4 ° C, 60 ⁇ 15% RH and under natural photoperiod. Newly emerged adult females less than 5 days old were used in all trials.
- Both side arms were connected through high-density polyethylene (HDPE) tubes to two identical glass jars (5 liter volume), each of which was connected to an air pump that produced a unidirectional bumidified air flow at 150 ml / min (Pérez-Hedo et al. Journal or Pest Science 3: 543 -554 (2015)).
- HDPE high-density polyethylene
- a female was introduced individually into the inlet tube of the olfactometer and observed until she had walked at least 3 cm on one of the arms of choice or until 15 minutes had elapsed. A total of 40 valid replicates were recorded for each species for each pair of odor sources. Each individual was tested only once. Females who did not choose a lateral arm within 15 minutes were recorded as "no response" and were excluded from data analysis. After recording five responses, tube Y was rinsed with soapy water and then cleaned with acetone and allowed to dry for 5 minutes. The odor sources were subsequently switched between the left and right side arms to minimize any spatial effect on the choice. The two types of plants (intact and induced) were used only once to assess the response of 10 females and then replaced with new plants. The Y-tube experiment was performed under the following environmental conditions: 23 ⁇ 2 ° C and 60 ⁇ 10% RH.
- the cages were evenly distributed at a distance of one and a half meters between them. Each cage represented a replica. Two portions of impregnated filter paper were placed on the bottom of each cage as explained above for both the (Z) -3-hexenyl propanoate treatment and the control. In the treatment where volatiles were exposed throughout the experiment, the impregnated filter paper portions were replaced every other day.
- T. absolute To evaluate the effect of exposure to both volatiles on T, absolute, two consecutive experiments were carried out. In the first, the effect on the laying of T. absolute was studied. The selected eggs laid in the first experiment were used later in the second to study the mortality of the immature T. absolute developed in plants exposed to (Z) -3-hexenyl propanoate, The same three treatments described above for T. urticae they were also tested for T. absolute. The laying of T. absolute was evaluated in 8 tomato plants (cv. Moneymaker) per treatment.
- Each of the plants was isolated inside a plastic cage (60 x 60 x 60 cm) (BugDorm-2) and kept in a climatic chamber at 25 ⁇ 2 ° C, 65 ⁇ 5% RH, 14:10 ( L: D) h photoperiod following the same test distribution described for T. urticae.
- a plastic cage 60 x 60 x 60 cm
- BugDorm-2 a plastic cage
- climatic chamber 25 ⁇ 2 ° C, 65 ⁇ 5% RH, 14:10 ( L: D)
- T. absolute To study the mortality of T. absolute in the plants exposed to the 3 treatments described above, 6 eggs of T. absolute per plant were distributed equally in all the leaves with the help of a fine brush. The eggs used in each treatment came from the corresponding treatment of the first experiment.
- the mortality of T. absolute was evaluated in 8 tomato plants (cv. Moneymaker) per treatment. Each of the plants was isolated inside a plastic cage (60 x 60 x 60 cm) (BugDorm-2 insect tents) kept in a climatic chamber at 25 ⁇ 2 ° C, 65 ⁇ 5% RH, 14:10 (L: D) h photoperiod following the same treatment distribution described above. The plants were left intact until the adults of T, absolute emerged. As soon as the adults began to emerge, they were counted and removed from the cages daily.
- the recorded weights (y) are related to the aging time (x) by means of a multiple regression analysis.
- the emitters were kept inside the corresponding booths throughout the test and samples were taken from the plants of each booth 4 weeks after the installation of the treatment. From these samples, the transcriptional response of the defensive PIN2 gene (a marker gene for the jasmonic acid signaling pathway, JA) was studied. Five repetitions per treatment were carried out. For the extraction and quantification of gene expression, the methodology described by Pérez-Hedo et al. Journal of Pest Science 3: 543-554 (2015).
- sequences of the primer used for the quantification of the PIN2 gene were direct (forward): 5'- GA AA AT CGTTAATTT AT CCC AC-3 '(SEG ID NO: 9) and inverse (reverse): 5'- ACATACAAACTTTCCATCTTTA-3' (SEQ ID NO: 10), while the EF1 constitutive gene sequences were forward: 5'-GATTGGTGGTATTGGAACTGTC-3 '(SEQ ID NO: 7) and reverse: 5G-AGCTT CGTGGTGCATCT C-30 (SEQ ID NO: 8) .
- Example 3 Defensive activation tests in tomato via exposure under field conditions
- Example 4 Defensive activation tests in citrus fruits via exposure under laboratory conditions
- Volatile (Z) -3-hexenyl propanoate was available from Sigma-Aidrich (St. Louis, MO, USA). The volatile was exposed by using a 2 x 2 cm portion of filter paper on which 10 ⁇ l of a solution containing the volatile corresponding to a concentration of 1: 10,000 [(Z) -3- propanoate was impregnated. bexenyl: methanol] as an emitting source of the corresponding volatile (Pérez-Hedo, M. et al. Biocontrol, 63: 203-213 (2016)).
- the nucleotides of the housekeeping gene gene glyceraldehyde 3-phosphate dehydrogenase were forward: 5'-GGAAGGTCAAGATCGGAATCAA-3 '(SEQ ID NO: 17) and reverse: 5'-CGTCCCTCTGCAAGATGACTCT-3 : (SEQ ID NO: 18).
- citriy females of the T. radiata parasitoid to Carrizo cltrange plants that were previously exposed for 24 ha (Z) -3-HP in relation to intact plants, a Y-shaped olfactometer of the same form that described in Example 1 and the plants were exposed to both volatiles as described above with the use of the filter paper portions.
- a total of 40 valid replicates were recorded for each species for each pair of odor sources. Each individual was tested only once.
- Females who did not choose a lateral arm within 15 minutes were recorded as "no response" and were excluded from data analysis. After recording five responses, tube Y was rinsed with soapy water and then cleaned with acetone and allowed to dry for 5 minutes.
- the odor sources were subsequently switched between the left and right side arms to minimize any spatial effect on the choice.
- the two types of plants (intact and induced) were used only once to assess the response of 10 females and then replaced with new plants.
- the Y-tube experiment was performed under the following environmental conditions: 25 ⁇ 1 ° C and 60 ⁇ 10% RH.
- the adults of D. citri were obtained from a colony established at SWFREC (University of Florida) whose original provenance was citrus fruits located in their experimental plots. The parasitodes were collected in these experimental fields. Newly emerged adult females less than 5 days old were used in all trials.
- Each seedling was distributed in the field following a random block design (using row of trees as a block) and in each block a seedling with a diffuser and another without was placed. The seedlings were located at a minimum distance of 50 meters between them to avoid any edge effect.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Dispersion Chemistry (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MX2022013232A MX2022013232A (es) | 2020-04-21 | 2021-04-20 | Uso de esteres de (z)-3-hexenilo y metodo para proteger plantas frente a plagas. |
EP21792498.4A EP4140302A4 (en) | 2020-04-21 | 2021-04-20 | USE OF (Z)-3-HEXENYL ESTERS AND METHOD FOR PROTECTING PLANTS AGAINST PARASITES |
US17/920,656 US20230240290A1 (en) | 2020-04-21 | 2021-04-20 | Use of (z)-3-hexenyl esters and method for protecting plants against pests |
CA3178669A CA3178669A1 (en) | 2020-04-21 | 2021-04-20 | Use of (z)-3-hexenyl esters and method for protecting plants against pests |
IL297478A IL297478A (en) | 2020-04-21 | 2021-04-20 | Use of esters of (g)-3-hexenyl and a method for protecting plants from pests |
BR112022021347A BR112022021347A2 (pt) | 2020-04-21 | 2021-04-20 | Uso de ésteres (z)-3-hexenila e método para proteger plantas contra pragas |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES202030330A ES2763224B2 (es) | 2020-04-21 | 2020-04-21 | Uso de ésteres de (Z)-3-hexenilo y método para proteger plantas frente a plagas |
ESP202030330 | 2020-04-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2021214360A1 true WO2021214360A1 (es) | 2021-10-28 |
Family
ID=70776988
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/ES2021/070259 WO2021214360A1 (es) | 2020-04-21 | 2021-04-20 | Uso de ésteres de (z)-3-hexenilo y método para proteger plantas frente a plagas |
Country Status (8)
Country | Link |
---|---|
US (1) | US20230240290A1 (es) |
EP (1) | EP4140302A4 (es) |
BR (1) | BR112022021347A2 (es) |
CA (1) | CA3178669A1 (es) |
ES (1) | ES2763224B2 (es) |
IL (1) | IL297478A (es) |
MX (1) | MX2022013232A (es) |
WO (1) | WO2021214360A1 (es) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106614583A (zh) * | 2016-12-12 | 2017-05-10 | 江苏省农业科学院 | 一种植物源引诱剂及其应用 |
CN110074143A (zh) * | 2019-05-08 | 2019-08-02 | 金华市农业科学研究院 | 一种引诱茶籽象甲成虫的组合物及其制备方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EA201390851A1 (ru) * | 2010-12-10 | 2014-01-30 | Оберн Юниверсити | Инокуляты, включающие бактерии bacillus для индукции продукции летучих органических соединений в растениях |
ES2623386B2 (es) * | 2017-05-11 | 2018-03-02 | Universitat Politècnica De València | Uso de un compuesto para la protección de plantas mediante cierre estomático y método de protección de plantas mediante cierre estomático que comprende aplicar a las plantas dicho compuesto |
-
2020
- 2020-04-21 ES ES202030330A patent/ES2763224B2/es active Active
-
2021
- 2021-04-20 CA CA3178669A patent/CA3178669A1/en active Pending
- 2021-04-20 WO PCT/ES2021/070259 patent/WO2021214360A1/es unknown
- 2021-04-20 EP EP21792498.4A patent/EP4140302A4/en active Pending
- 2021-04-20 BR BR112022021347A patent/BR112022021347A2/pt unknown
- 2021-04-20 US US17/920,656 patent/US20230240290A1/en active Pending
- 2021-04-20 IL IL297478A patent/IL297478A/en unknown
- 2021-04-20 MX MX2022013232A patent/MX2022013232A/es unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106614583A (zh) * | 2016-12-12 | 2017-05-10 | 江苏省农业科学院 | 一种植物源引诱剂及其应用 |
CN110074143A (zh) * | 2019-05-08 | 2019-08-02 | 金华市农业科学研究院 | 一种引诱茶籽象甲成虫的组合物及其制备方法 |
Non-Patent Citations (12)
Title |
---|
BOUAGGA ET AL., JOURNAL OF PEST SCIENCE, vol. 91, 2018, pages 55 - 64 |
BOUAGGA ET AL., PEST MANAGEMENT SCIENCE, vol. 76, 2020, pages 561 - 567 |
ENGELBERTH, J.H. T. ALBORN, PROC. NATL. ACAD. SCI., vol. 101, 2004, pages 1781 - 1785 |
GASMI LAILA, MARTÍNEZ-SOLÍS MARÍA, FRATTINI ADA, YE MENG, COLLADO MARÍA CARMEN, TURLINGS TED C. J., ERB MATTHIAS, HERRERO SALVADOR: "Can Herbivore-Induced Volatiles Protect Plants by Increasing the Herbivores’ Susceptibility to Natural Pathogens?", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 85, no. 1, 1 January 2019 (2019-01-01), US , pages e01468-18, XP055868247, ISSN: 0099-2240, DOI: 10.1128/AEM.01468-18 * |
GEORG VON MREY; NATHALIE VEYRAT; GEORGE MAHUKU; RAYMUNDO LOPEZ VALDEZ; TED C.J. TURLINGS; MARCO DALESSANDRO;: "Dispensing synthetic green leaf volatiles in maize fields increases the release of sesquiterpenes by the plants, but has little effect on the attraction of pest and beneficial insects", PHYTOCHEMISTRY, ELSEVIER, AMSTERDAM , NL, vol. 72, no. 14, Amsterdam , NL , pages 1838 - 1847, XP028256720, ISSN: 0031-9422, DOI: 10.1016/j.phytochem.2011.04.022 * |
GIRON-CALVA PATRICIA SARAI; LI TAO; BLANDE JAMES D.: "Volatile-Mediated Interactions between Cabbage Plants in the Field and the Impact of Ozone Pollution", JOURNAL OF CHEMICAL ECOLOGY, SPRINGER, NL, vol. 43, no. 4, 29 March 2017 (2017-03-29), NL , pages 339 - 350, XP036216348, ISSN: 0098-0331, DOI: 10.1007/s10886-017-0836-x * |
PEREZ-HEDO ET AL., JOURNAL OF PEST SCIENCE, vol. 3, 2015, pages 543 - 554 |
PéREZ-HEDO MERITXELL; RAMBLA JOSE LUIS; GRANELL ANTONIO; URBANEJA ALBERTO: "Biological activity and specificity of Miridae-induced plant volatiles", BIOCONTROL, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, NL, vol. 63, no. 2, 23 November 2017 (2017-11-23), NL , pages 203 - 213, XP036456224, ISSN: 1386-6141, DOI: 10.1007/s10526-017-9854-4 * |
PEREZ-HEDO, M. ET AL., BIOCONTROL, vol. 63, 2018, pages 203 - 213 |
See also references of EP4140302A4 |
TURLINGS, T.C.J.ERB, M., ANNU. REV. ENTOMOL., vol. 63, 2018, pages 433 - 452 |
URBANEJA-BERNAT ET AL., JOURNAL OF APPLIED ENTOMOLOGY, vol. 139, 2015, pages 61 - 167 |
Also Published As
Publication number | Publication date |
---|---|
MX2022013232A (es) | 2023-01-24 |
EP4140302A1 (en) | 2023-03-01 |
CA3178669A1 (en) | 2021-10-28 |
IL297478A (en) | 2022-12-01 |
ES2763224A1 (es) | 2020-05-27 |
ES2763224B2 (es) | 2021-02-23 |
US20230240290A1 (en) | 2023-08-03 |
EP4140302A4 (en) | 2024-05-22 |
BR112022021347A2 (pt) | 2023-01-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Smart et al. | Role of semiochemicals in integrated pest management | |
Navarro-Llopis et al. | Mass trapping for fruit fly control | |
Tremblay et al. | Effects of bird predation on some pest insect populations in corn | |
JP6467177B2 (ja) | 花の香り由来の天敵昆虫誘引成分およびその利用 | |
Raja et al. | Efficacy of the entomopathogenic nematode Heterorhabditis bacteriophora using different application methods in the presence or absence of a natural enemy | |
Sanda et al. | Biological invasion of tomato leaf miner, Tuta absoluta (Meyrick) in Nigeria: problems and management strategies optimization: a review | |
Sampson | Management of the western flower thrips on strawberry | |
ES2351449A1 (es) | Procedimiento para la producción de una especie de parasitoide autóctono y utilización del mismo para el control de lepidópteros plaga de cultivos. | |
Leon et al. | Seed production and control of sicklepod (Senna obtusifolia) and pitted morningglory (Ipomoea lacunosa) with 2, 4-D, dicamba, and glyphosate combinations | |
ES2763224B2 (es) | Uso de ésteres de (Z)-3-hexenilo y método para proteger plantas frente a plagas | |
Hashemi | Influence of Pheromone Trap Color and Placement on Catch of Male Potato Tuber Moth, Phthorimaea operculella (Zeller, 1873). | |
KR100421779B1 (ko) | 한국산 곤충병원성 선충 및 그에 의한 방제법 | |
Huiting et al. | Biology, control and luring of the cockchafer, Melolontha melolontha: literature report on biology, life cycle and pest incidence, current control possibilities and pheromones | |
Otieno | Integration of soil-applied azadirachtin with predators, entomopathogens and optical/chemical traps for the management of western flower thrips, Frankliniella occidentalis Pergande (Thysanoptera: Thripidae) | |
Montemayor et al. | Is UV-C light compatible with biological control of twospotted spider mite? | |
KR101102113B1 (ko) | 친환경적인 녹지공간 병·해충관리 시스템 및 방법 | |
Cutler | Integrated pest management (IPM): from theory to application | |
Marsh | Use of Curry Leaf (Murraya koenigii) and Volkamer Lemon (Citrus volkameriana) as Potential Trap Crops for the Asian Citrus Psyllid (Diaphorina citri) in a Commercial Citrus Grove | |
Kwon et al. | An Evidence to Use Cotes/a plutellae (Kurdjumov)(Hymenoptera: Braconidae) as a Field Control Agent against Diamondback Moth, Plutella xylostella L (Lepidoptera: Plutellidae) | |
Skënderasi et al. | MONITORING THE BIOLOGY OF THE CORN ROOTWORM (DIABROTICA VIRGIFERA) FOR THE REGION OF KORÇA | |
Puppato | Development of new tools for an agroecological management of spotted wing drosophila, Drosophila suzukii | |
Nishantha et al. | Moth Repellent Yellow-Specific Bulbs for the Management of Lepidopteran Pod Borers and Leaf Eating Caterpillars on Vegetable Pest Management Programs in Sri Lanka | |
Baruah et al. | Chapter-6 Insect Pest Management in Organic Farming: An Indian Perspective | |
UA134096U (uk) | Інтегрований метод біозахисту рослин з використанням комплексу ентомофагів (chrysopa cornea steph, trichogramma, habrobracon hebertor say) | |
Hazir et al. | Efficacy of the entomopathogenic nematode Heterorhabditis bacteriophora using different application... |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 21792498 Country of ref document: EP Kind code of ref document: A1 |
|
REG | Reference to national code |
Ref country code: BR Ref legal event code: B01A Ref document number: 112022021347 Country of ref document: BR |
|
ENP | Entry into the national phase |
Ref document number: 3178669 Country of ref document: CA |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
ENP | Entry into the national phase |
Ref document number: 2021792498 Country of ref document: EP Effective date: 20221121 |
|
REG | Reference to national code |
Ref country code: BR Ref legal event code: B01E Ref document number: 112022021347 Country of ref document: BR Free format text: 1) FAVOR EFETUAR, EM ATE 60 (SESSENTA) DIAS, O PAGAMENTO DE GRU CODIGO DE SERVICO 260 PARAA REGULARIZACAO DO PEDIDO, CONFORME ART 2O 1O DA RESOLUCAO 189/2017 E NOTA DEESCLARECIMENTO PUBLICADA NA RPI 2421 DE 30/05/2017, UMA VEZ QUE A PETICAO NO 870220108774DE 23/11/2022 APRESENTA DOCUMENTOS REFERENTES A 3 SERVICOS DIVERSOS (COMPLEMENTACAO DATRADUCAO, MODIFICACAO DE PAGINAS JA ENVIADAS NO PEDIDO E APRESENTACAO DE PROCURACOES) TENDOSIDO PAGA SOMENTE 2 RETRIBUICAO. DEVERA SER PAGA MAIS 1 (UMA) GRU CODIGO DE SERVICO 260ALEM DO PAGAMENTE DA GRU 207 DE RESPOSTA A ESSA EXIGENCIA. 2) APRESENTE NOVO CONTEUDOELETRONICO DE LISTAGEM DE SEQUENCIAS BIOLOGICAS, UMA VEZ QUE O APRESENTADO NA PETICAO NO870220 |
|
ENP | Entry into the national phase |
Ref document number: 112022021347 Country of ref document: BR Kind code of ref document: A2 Effective date: 20221020 |