WO2021190131A1 - 一种有色稻三系杂交组合的选育方法 - Google Patents

一种有色稻三系杂交组合的选育方法 Download PDF

Info

Publication number
WO2021190131A1
WO2021190131A1 PCT/CN2021/074373 CN2021074373W WO2021190131A1 WO 2021190131 A1 WO2021190131 A1 WO 2021190131A1 CN 2021074373 W CN2021074373 W CN 2021074373W WO 2021190131 A1 WO2021190131 A1 WO 2021190131A1
Authority
WO
WIPO (PCT)
Prior art keywords
line
rice
colored
hybrid
colored rice
Prior art date
Application number
PCT/CN2021/074373
Other languages
English (en)
French (fr)
Inventor
吴殿星
陈霄雨
章思妍
Original Assignee
浙江大学
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 浙江大学 filed Critical 浙江大学
Publication of WO2021190131A1 publication Critical patent/WO2021190131A1/zh

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

Definitions

  • the invention belongs to the technical field of rice breeding, and specifically relates to a breeding method for a three-line hybrid combination of colored rice.
  • Colored rice refers to rice with brown rice color, mainly black, purple, red, yellow, green, etc. Colored rice has more complete nutrition and contains physiologically active substances that can prevent the occurrence of various chronic diseases, making it a healthy choice for many consumers.
  • China's colored rice is rich in germplasm resources; there are both colored rice, mainly conventional rice varieties, and a small number of colored hybrid rice varieties, such as Dianza 701, Jinnong 3 You 3, Jin You Hong, and Gaoyou Red 88, Yuyou Red 9, II You 132, Special Red 1256, black rice like Heiyou No. 1.
  • Both conventional and hybrid varieties of colored rice are colored rice with a single color.
  • the colorful rice seen in the market is a mixture of various colored rice, which has the problem of uneven quality and taste, which is not only inconvenient for consumption. The daily consumption of consumers is also not conducive to the processing and application of enterprises.
  • the present invention proposes a breeding method for colored rice three-cross combinations, aiming to provide a natural multicolored rice, so that the nutrition of the rice is more complete and the quality is more uniform.
  • the purpose of the present invention is to provide a breeding method for a three-line hybrid combination of colored rice.
  • the three-line hybrid rice obtained by the breeding method not only has five-color brown rice, but also has the advantages of high yield, high quality and disease resistance. .
  • a method for breeding a three-line hybrid combination of colored rice which includes the following steps:
  • A) to D colored rice male parent is female restorer lines R, hybridization, the obtained backcross progeny with the parent to obtain BC m F '1 substituting, BC m F' after selfing generation, harvested BC m F '2 generation seeds;
  • hybrid F. 1A Three Sterile rice restorer lines obtained by test cross hybrids F 1 HETEROSIS, having selected heterosis hybrid F 1 generation, referred to as a hybrid F. 1A; and hybrids. 1A as F corresponding parent colored rice three Sterile S 'and colored rice restorer line R' 3 retest, until when the plants mature, to obtain hybrid F 1b; brown color was observed and the quality of hybrid F 1b, selecting a brown color and five F 1b hybrid quality restorer lines corresponding to R '4;
  • the "B' 1 ⁇ B' 4 "in the above breeding methods are all maintainer plant populations obtained during the breeding process, and the number of plants are all >1;
  • S' 1 are all sterile line plants obtained during the breeding process Population, the number of plants>1;“R' 1 ⁇ R' 4 ”are the restorer line plant populations obtained during the breeding process, and the number of plants are all>1; and
  • B'”, “S'” and “R'” are all They are 1 maintainer plant, 1 sterile plant and 1 restorer plant obtained after selection and breeding.
  • the colored rice C and colored rice D are any two of black rice rice, red rice rice and purple rice rice.
  • the colored rice C is Zhongze Bankhen; the colored rice D is Zhongze Hongzhen.
  • Zhongze Bankhen and Zhongze Hongzhen black rice and red rice varieties jointly cultivated by Zhejiang University and Zhongze Biotechnology Co., Ltd., have applied for the protection of new plant varieties to the Ministry of Agriculture (application number: 20150962.5), which can be purchased from China Ze Biological Technology Co., Ltd.
  • the three-line sterile line S, the three-line maintainer line B, and the three-line restorer line R are conventional high-quality, high-yield three-line hybrid rice combinations.
  • the three-line sterile line S is Tianfeng A; the three-line maintainer line B is Tianfeng B; the three-line restorer line R is Huazhan; and the three-line hybrid rice is Tianyou Huazhan.
  • Tianyou Huazhan is a new high-quality and high-yield rice variety with Grade II quality selected by Zhejiang University, which can be obtained as a gift or purchased from Zhejiang University.
  • the sterile line Tianfeng A, the maintainer line Tianfeng B, and the restorer line R Huazhan were selected by China Rice Research. They can be obtained as a gift or purchased from the China National Rice Research Institute, and can also be obtained from the National Rice Germplasm Bank.
  • the overall evaluation of sterility, combining ability, yield, quality and resistance, etc. was selected to breed colored rice sterile line S', maintainer line B', restorer line R'and three-cross combination S' /B' ⁇ R', the specific requirements include: 1) S'and S have similar sterile characteristics, meet the three-line sterile line identification standards, have high outcrossing seed setting rate and seed production; 2) R'and R The restoration characteristics are similar, and the combining ability, yield, quality and resistance levels are similar to R; 3) The yield, quality and resistance levels of S'/B' ⁇ R are similar to the target hybrid rice S ⁇ R.
  • step a) the n is 3 to 5 times; in step A), m is 3 to 5 times.
  • the characteristics of the sterile line are that the outcrossing seed setting rate is ⁇ 40%; the seed production yield is ⁇ 200kg.
  • the high quality is that the quality of rice reaches the national third-level or above standard; in step C), the high yield is the yield ⁇ 500 kg/mu, and the high resistance is the resistance to rice blast; In II), the yield is ⁇ 500kg/mu, the quality of rice reaches the national third-level and above standards, and the medium resistance to bacterial blight is used as the criterion for heterosis.
  • the present invention has the following beneficial effects:
  • the present invention uses colored rice and conventional high-quality, high-yielding three-line hybrid rice combinations to obtain colored rice three-line sterile lines, colored rice three-line maintainers and colored rice three-line restorers, and finally obtained colored rice three A new combination of hybrid rice, the colored rice three-line hybrid rice not only has five-color brown rice, but also has the advantages of high yield, high quality and disease resistance;
  • the method of the present invention provides a natural five-color colored rice three-line hybrid rice, which is significantly different from the conventional single-color colored rice varieties or hybrids currently produced, and changes the so-called multicolored rice on the market.
  • the present invention specifically selects the target three-line hybrid rice combination, and the selected colored three-line hybrid rice has the characteristics of high-quality, high-yield and adaptability. While the rice can be used for both, it can significantly reduce the risk of popularization, and it has high iron content and high iron content. Low cadmium content is more beneficial to human health.
  • breeding methods provided in the following examples are based on the high-quality, high-yielding target three-line hybrid rice combination A (Tianyou Huazhan, three-line sterile line Tianfeng A and maintainer line Tianfeng B, restorer line R Huazhan) Breeding method of three-line hybrid combination of colored rice.
  • the sterile line Tianfeng A was bred by the Guangdong Academy of Agricultural Sciences. It has excellent performance, good combining ability, moderate resistance to rice blast and moderate bacterial blight; the restorer line R Huazhan was bred by China Rice Research , The performance is excellent, the combining ability is good, high resistance to rice blast, medium resistance to bacterial blight.
  • Tianyou Huazhan is a new high-quality and high-yielding rice variety selected by the China Rice Research Institute with a second-level breeding quality. It can be obtained as a gift or purchased from the China National Rice Research Institute.
  • the sterile line Tianfeng A, the maintainer line Tianfeng B and the restorer line R Huazhan can be obtained as gifts or purchased from the Guangdong Academy of Agricultural Sciences or the Chinese Rice Research Institute respectively, or can be obtained from the National Rice Germplasm Bank.
  • Colored rice C Zhongze Bankhen and red rice Colored rice D Zhongze Hongzhen is a black rice and red rice variety jointly cultivated by Zhejiang University and Zhongze Biotechnology Co., Ltd., and has applied for the protection of new plant varieties to the Ministry of Agriculture (application number: 20150962.5), which can be purchased from Zhongze Biological Technology Co., Ltd.
  • a method for breeding a three-line hybrid combination of colored rice the specific steps are as follows:
  • sterile lines S '1 + black, dark brown, white, etc. indoor sterility identification, selected from the left sterile line characteristics meet sterility (CMS line characteristics outcrossing rate ⁇ 40%; seed production yield ⁇ 200kg) of a colored rice sterile line S '+ black, dark brown, white, etc., there is obtained a colored rice sterile lines S' + black, dark brown, white, etc., and a The corresponding colored rice maintainer line B' + black, dark brown, white, etc.
  • A) is colored red rice Nakazawa Zhen D male parent, R restorer lines as female Chinese accounted for, hybridization, and the obtained progeny backcross parent China accounted for 4 times to obtain BC 4 F '1 on behalf of, the BC 4 F 'after selfing generation, harvesting BC 4 F' 2 generation seeds;
  • seed 136 is selected from colored planting, exhibits brown colored white progeny isolated restorer trait when R is selected from harvested left' 1 plant 72;
  • restorer R 'agronomic traits of a plant investigated restorer R 'agronomic traits of a plant, plant height and select are consistent growth of restorer lines 25 R' plant 1, for yield, rice quality and disease resistance evaluation, selection brown Characters appear separate colored and white high-quality (up to national levels of quality and above standard quality of rice), high yield (high yield for the yield ⁇ 500kg / acre) and high resistance (high resistance to blast resistance) restorers R '2 + 16 in red, light red, white, etc.
  • a step e) is obtained in a colored rice sterile line S '+ black, dark brown, and white, respectively restorer lines 16 R' 2 + red, light red, white paired test cross hybrids obtained F 1 generation;
  • colored rice three-line hybrid rice X + black, dark brown, red, light red, white, etc. namely: S' + black, dark brown, white , etc./B' + black, dark brown, white, etc. ⁇ R' + Red, light red, white, etc. , Tianfeng A/Tianfeng B + black, dark brown, white, etc. ⁇ Hua Zhan + red, light red, white, etc .; colored rice three-line sterile line S' + black, dark brown, White, etc. , namely: Tianfeng A + black, dark brown, white, etc .; colored rice maintainer line B' + black, dark brown, white, etc. , namely: Tianfeng B + black, dark brown, white, etc .; restoration line R' + red, light red, white and so on, namely: China accounted for + red, light red, white and so on.
  • the grade comparison test showed that the color rice three-cross hybrid combination was bred, and the brown rice has a rich and colorful appearance, including black, dark brown, red, light red, various intermediate colors, and white, etc., and its population yield, quality and resistance levels are the same.
  • Tianyou Huazhan is comparable, and the yield is significantly higher than Zhongze Bankhen and Zhongze Hongzhen, the resistance is significantly improved, the quality is similar, and the quality, resistance and yield are significantly better than the hybrid combination Tianfeng A ⁇ Zhongze Bankhen You, Tianfeng A ⁇ Zhongze Hongzhen.
  • the colored rice three-cross hybrid combination Tianfeng A + black rice, dark brown, white, etc. ⁇ Hua Zhan + red rice, light red, white, etc. micronutrient iron and heavy metal cadmium
  • the iron content is the highest, and the cadmium content is the lowest.
  • Iron is one of the most important micronutrients, which has important functions of replenishing blood and preventing anemia; and cadmium is the most typical harmful heavy metal, and it is also the most concerned public nutrition.
  • the high iron content and low cadmium content indicate that the selected varieties of the present invention have health care and are safer to eat, and are more beneficial to human health.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

提供了一种有色稻三系杂交组合的选育方法，包括：以有色稻C为父本，三系保持系B为母本，进行杂交和回交，并进行有色稻三系保持系和三系不育系的选育；以有色稻D为父本，恢复系R为母本，进行杂交和回交，并选育有色稻恢复系；再通过杂种优势的筛选、稻米品质、产量和抗性的筛选，获得有色稻三系杂交品种新组合，构建了有色稻三系杂交体系。

Description

一种有色稻三系杂交组合的选育方法
本申请要求于2020年03月23日提交中国专利局、申请号为202010206146.7、发明名称为“一种有色稻三系杂交组合的选育方法”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
技术领域
本发明属于水稻育种技术领域，具体涉及一种有色稻三系杂交组合的选育方法。
背景技术
有色稻米是指糙米具有色泽的稻米，主要有黑、紫、红、黄、绿色等。有色稻米因营养更齐全，并含有可以预防各种慢性疾病的发生的生理活性物质，成为众多消费者的健康选择。
中国有色稻种质资源丰富；既有以常规水稻品种为主的有色稻，又有少量的有色稻杂交稻品种，红米如滇杂701、金农3优3号、金优红、高优红88、渝优红9、Ⅱ优132、特优红1256，黑米如黑优1号。无论是有色稻的常规品种还是杂交品种，都是单种颜色的有色米，市场所见的五彩米均是由各种有色米混合而成，存在品质与口感不均一的问题，不但不方便消费者的日常食用，也不利于企业的加工应用。
发明内容
为此，本发明提出一种有色稻三交组合的选育方法，旨在提供一种天然五彩米，使稻米的营养更齐全，品质更均一。
有鉴于此，本发明的目的在于提供一种有色稻三系杂交组合的选育方法，该选育方法获得的三系杂交水稻不仅具有五彩色的糙米，而且具有高产、优质、抗病的优点。
为了实现本发明的这些目的和其它优点，提供了一种有色稻三系杂交组合的选育方法，包括以下步骤：
(1)有色稻三系保持系与三系不育系的选育：
a)以有色稻C为父本，保持系B为母本，进行杂交，获得的子代与母本进行回交，得到BC nF 1代，BC nF 1代自交后，收获BC nF 2代的种子；
b)观察BC nF 2代种子的糙米外观，选有色的种子进行种植，选留收获时子代糙米呈现有色与白色性状分离的保持系B' 1单株；
c)考察保持系B' 1单株的农艺性状，选择其中株高和生育期均一致的保持系B' 2单株，检测保持系B' 2单株的稻米品质，选留糙米呈现有色与白色性状分离的优质的保持系B' 3；
d)将三系不育系S与保持系B' 3成对测交和复测，获得子代植株S/B' 3，查看并选留完全不育的子代植株所对应的保持系B' 4，生产保持系B' 4相对应的不育系S' 1；
e)对不育系S' 1进行室内外育性鉴定，选留不育性符合不育系特性的有色稻不育系S'，获得有有色稻三系不育系S'及其相对应的有色稻保持系B'；
(2)有色稻恢复系的选育：
A)以有色稻D为父本，恢复系R为母本，进行杂交，获得的子代与母本进行回交，得到BC mF' 1代，BC mF' 1代自交后，收获BC mF' 2代的种子；
B)观察BC mF' 2代种子的糙米外观，选有色的种子进行种植，选留收获时子代糙米呈现有色与白色性状分离的恢复系R' 1单株；
C)考察恢复系R' 1单株的农艺性状，选择其中株高和生育期均一致的恢复系R' 1单株，进行产量、稻米品质及病害抗性的评价，选择糙米呈现有色与白色性状分离的优质、高产和高抗的恢复系R' 2；
(3)有色稻三系杂交品种新组合的获得：
I)将步骤e)中获得的有色稻不育系S'分别与恢复系R' 2成对测交，获得杂种F 1代；
II)鉴定有色稻三系不育系和有色稻恢复系测交后获得的杂种F 1代的杂种优势，选择具有杂种优势的杂种F 1代，记为杂种F 1a；并将杂种F 1a所对应的亲本有色稻三系不育系S'和有色稻恢复系R' 3进行复测，待植株成熟时，获得杂种F 1b；观察杂种F 1b的糙米颜色和品质，选择具有五彩 色糙米且优质的杂种F 1b所对应恢复系R' 4；
(4)繁制种生产：将步骤II)获得的有色稻不育系S'与恢复系R' 4制种，终选繁制种1个产量高的恢复系R'；
(5)建立有色稻三系杂交稻S'/B'×R'、有色稻三系不育系S'、有色稻保持系B'和恢复系R'构成的有色稻三系杂交体系。
上述选育方法中涉及的“B' 1～B' 4”均为选育过程中获得保持系植株群体，植株数量均＞1；“S' 1”均为选育过程中获得不育系植株群体，植株数量＞1；“R' 1～R' 4”均为选育过程中获得恢复系植株群体，植株数量均＞1；而“B'”、“S'”和“R'”均为选育完成后获得的1个保持系植株、1个不育系植株和1个恢复系植株。
进一步地，所述有色稻C和有色稻D为黑米水稻、红米水稻和紫米水稻中的任意两种。
进一步地，所述有色稻C为中泽黑珍；所述有色稻D为中泽红珍。
中泽黑珍和中泽红珍，是浙江大学与中泽生物科技有限公司联合培育的黑米与红米品种，已向农业部申请植物新品种保护(申请号：20150962.5)，可购于中泽生物科技有限公司。
进一步地，所述三系不育系S、三系保持系B和三系恢复系R为常规优质、高产的三系杂交稻组合。
进一步地，所述三系不育系S为天丰A；所述三系保持系B为天丰B；所述三系恢复系R为华占；三系杂交稻为天优华占。
其中，天优华占是浙江大学选育米质二级的优质高产水稻新品种，可从浙江大学惠赠得到或购买得到。
不育系天丰A、保持系天丰B和恢复系R华占，为中国水稻研究所选育，可从中国水稻研究所惠赠得到或购买得到，也可向国家水稻种质资源库索取。
按照水稻育种技术和流程，对不育性、配合力、产量、品质与抗性等总体评价，选育有色稻不育系S'，保持系B'、恢复系R'及三交组合S'/B'×R'，具体要求包括：l)S'与S的不育特性类似，符合三系不育系鉴定标准，具有高异交结实率和制种产量；2)R'与R的恢复特性类似，配合力、产量、 品质和抗性等水平与R相仿；3)S'/B'×R的产量、品质和抗性等水平与目标杂交水稻S×R相仿。
进一步地，步骤a)中，所述n为3～5次；步骤A)中，m为3～5次。
进一步地，步骤e)中，所述不育系特性为异交结实率≥40％；繁制种产量≥200kg。
进一步地，步骤C)、II)中，所述优质为稻米品质达国家三级及以上标准；步骤C)中，所述高产为产量≥500kg/亩，所述高抗为抗稻瘟病；步骤II)中，以产量≥500kg/亩，稻米品质达国家三级及以上标准，中抗白叶枯病作为具有杂种优势的评判标准。
与现有技术相比，本发明具有以下有益效果：
(1)本发明采用有色稻与常规优质、高产的三系杂交稻组合进行杂交分别获得有色稻三系不育系、有色稻三系保持系和有色稻三系恢复系，最终获得有色稻三系杂交水稻新组合，该有色稻三系杂交稻不仅具有五彩色的糙米，而且具有高产、优质、抗病的优点；
(2)本发明方法提供了一种天然五彩色的有色稻三系杂交稻，显著不同于目前生产的单种颜色的有色稻常规品种或杂交品种，改变了市场上所谓的五彩米均由各种有色米混合而成的现状；其营养更齐全，品质更均一，有利于蒸煮食用及深加工应用；
(3)本发明针对性选择了目标三系杂交水稻组合，选育的有色稻三系杂交稻具有优质、高产及适应性的特点，在稻米可兼用显著降低推广风险的同时，铁含量高、镉含量低更有益于人类的健康。
具体实施方式
下面结合具体实施例对本发明作进一步描述，以下列举的仅是本发明的具体实施例，但本发明的保护范围不仅限于此。
下列实施例中提供的育种方法是以优质、高产的目标三系杂交水稻组合A(天优华占，三系不育系天丰A与保持系天丰B，恢复系R华占)为基础的有色稻三系杂交组合的选育方法。
其中，不育系天丰A，为广东省农业科学院选育，表现品质优，配合力好，中抗稻瘟病，中抗白叶枯病；恢复系R华占，为中国水稻研究所 选育，表现品质优，配合力好，高抗稻瘟病，中抗白叶枯病。天优华占是中国水稻研究所选育米质二级的优质高产水稻新品种，可从中国水稻研究所惠赠得到或购买得到。
不育系天丰A、保持系天丰B和恢复系R华占，可分别从广东省农业科学院或中国水稻研究所惠赠得到或购买得到，也可向国家水稻种质资源库索取。
有色稻C中泽黑珍和红米有色稻D中泽红珍是浙江大学与中泽生物科技有限公司联合培育的黑米与红米品种，已向农业部申请植物新品种保护(申请号：20150962.5)，可购于中泽生物科技有限公司。
实施例1
一种有色稻三系杂交组合的选育方法，具体步骤如下：
(1)有色稻三系保持系与不育系的选育：
a)以有色稻C中泽黑珍为父本，保持系B天丰B为母本，进行杂交，获得的子代与母本天丰B回交4次，得到BC 4F 1代，BC 4F 1代自交后，收获BC 4F 2代的种子；
b)观察342粒BC 4F 2代种子的糙米外观，选有色的123粒种子进行种植，选留收获时子代糙米呈现有色与白色性状分离的保持系B' 1单株49株；
c)考察49株保持系B' 1单株的农艺性状，选择其中株高和生育期均一致的23个保持系B' 2单株，检测保持系B' 2单株的稻米品质，选留糙米呈现有色与白色性状分离的优质保持系B' 3 +黑色、黑褐色、白色等17个(上角标为稻米颜色，即获得的有色稻保持系上的籽粒带有黑色、黑褐色、白色等)；
d)将三系不育系S天丰A与保持系B' 3 +黑色、黑褐色、白色等成对测交和复测，获得子代植株S/B' 3 +黑色、黑褐色、白色等，查看并选留完全不育的子代植株所对应的保持系B' 4 +黑色、黑褐色、白色等11个，生产保持系B' 4 +黑色、黑褐色、白色等相对应的不育系S' 1 +黑色、黑褐色、白色等；
e)对不育系S' 1 +黑色、黑褐色、白色等进行室内外育性鉴定，选留不育性符合不育系特性(不育系特性为异交结实率≥40％；繁制种产量≥200kg)的1个有色稻不育系S' +黑色、黑褐色、白色等，获得有1个有色稻三系不育系S' +黑色、黑褐色、白 色等及其1个相对应的有色稻保持系B' +黑色、黑褐色、白色等。
(2)有色稻恢复系的选育：
A)以有色稻D中泽红珍为父本，恢复系R华占为母本，进行杂交，获得的子代与母本华占回交4次，得到BC 4F' 1代，BC 4F' 1代自交后，收获BC 4F' 2代的种子；
B)观察303粒BC 4F' 2代种子的糙米外观，选有色的136粒种子进行种植，选留收获时子代糙米呈现有色与白色性状分离的恢复系R' 1单株72株；
C)考察72株恢复系R' 1单株的农艺性状，选择其中株高和生育期均一致的25个恢复系R' 1单株，进行产量、稻米品质及病害抗性的评价，选择糙米呈现有色与白色性状分离的优质(优质为稻米品质达国家三级及以上标准)、高产(高产为产量≥500kg/亩)和高抗(高抗为抗稻瘟病)的恢复系R' 2 +红色、浅红色、白色等16个。
(3)有色稻三系杂交品种新组合的获得：
I)将步骤e)中获得的1个有色稻不育系S' +黑色、黑褐色、白色等分别与16个恢复系R' 2 +红色、浅红色、白色等成对测交，获得杂种F 1代；
II)鉴定有色稻三系不育系和有色稻恢复系测交后获得的杂种F 1代的杂种优势(以产量≥500kg/亩，稻米品质达国家三级及以上标准，中抗白叶枯病作为具有杂种优势的评判标准)，选择具有杂种优势的9个杂种F 1代，记为杂种F 1a；并将杂种F 1a所对应的亲本有色稻三系不育系S' +黑色、黑 褐色、白色等和有色稻恢复系R' 3 +红色、浅红色、白色等进行复测，待植株成熟时，获得杂种F 1b；观察杂种F 1b的糙米颜色和品质，选择具有五彩色糙米且优质(优质为稻米品质达国家三级及以上标准)的杂种F 1b所对应恢复系R'4 +红色、浅红 色、白色等5个；
(4)繁制种生产：将步骤II)获得的1个有色稻不育系S' +黑色、黑褐色、白色等与5个恢复系R' 4 +红色、浅红色、白色等制种，终选繁制种产量高的1个恢复系R' + 红色、浅红色、白色等。
(5)建立有色稻三系杂交稻X +黑色、黑褐色、红色、浅红色、白色等、有色稻三系不育系S' +黑色、黑褐色、白色等、有色稻保持系B' +黑色、黑褐色、白色等和恢复系R' +红色、浅红色、白色等 构成的有色稻三系杂交体系。
其中，有色稻三系杂交稻X +黑色、黑褐色、红色、浅红色、白色等，即：S' +黑色、黑褐色、白色 等/B' +黑色、黑褐色、白色等×R' +红色、浅红色、白色等，天丰A/天丰B +黑色、黑褐色、白色等×华占 +红色、 浅红色、白色等；有色稻三系不育系S' +黑色、黑褐色、白色等，即：天丰A +黑色、黑褐色、白色等；有色稻保持系B' +黑色、黑褐色、白色等，即：天丰B +黑色、黑褐色、白色等；恢复系R' +红色、浅红 色、白色等，即：华占 +红色、浅红色、白色等。
经品比试验，表明选育的有色稻三交杂交组合，糙米外观丰富多彩，有黑色、黑褐色、红色、浅红、各种中间色以及白色等，其群体产量、品质和抗性水平同天优华占相当，比中泽黑珍和中泽红珍的产量显著提高，抗性显著提升，品质相仿，品质、抗性与产量显著优于杂交组合天丰A×中泽黑珍优、天丰A×中泽红珍。
与原来的品种及配制的杂交稻组合相比，有色稻三交杂交组合天丰A +黑米、黑褐色、白色等×华占 +红米、浅红、白色等的微营养铁与重金属镉的含量上，凸显出超亲本及组合的优势，即与其他应用的品种相比，铁含量最高，而镉含量最低。铁是最重要微营养之一，具有重要的补血及预防贫血的功能；而镉是最典型有害重金属，也是目前公众营养最为担心的。铁含量高、镉含量低，说明本发明选育的品种具有保健的同时食用更为安全性，更有益于人的健康。
各个品种糙米颜色、产量、品质和抗性的具体比较见表1。
表1天优华占目标改良多彩有色稻三交杂交组合的指标性状比较
Figure PCTCN2021074373-appb-000001
Figure PCTCN2021074373-appb-000002
尽管本发明的实施方案已公开如上，但其并不仅仅限于说明书和实施方式中所列运用，它完全可以被适用于各种适合本发明的领域，对于熟悉本领域的人员而言，可容易地实现另外的修改，因此在不背离权利要求及等同范围所限定的一般概念下，本发明并不限于特定的细节和这里示出与描述的实例。

Claims (8)

  1. 一种有色稻三系杂交组合的选育方法，其特征在于，包括以下步骤：
    (1)有色稻三系保持系与三系不育系的选育：
    a)以有色稻C为父本，保持系B为母本，进行杂交，获得的子代与母本进行回交，得到BC nF 1代，BC nF 1代自交后，收获BC nF 2代的种子；
    b)观察BC nF 2代种子的糙米外观，选有色的种子进行种植，选留收获时子代糙米呈现有色与白色性状分离的保持系B' 1单株；
    c)考察保持系B' 1单株的农艺性状，选择其中株高和生育期均一致的保持系B' 2单株，检测保持系B' 2单株的稻米品质，选留糙米呈现有色与白色性状分离的优质的保持系B' 3；
    d)将三系不育系S与保持系B' 3成对测交和复测，获得子代植株S/B' 3，查看并选留完全不育的子代植株所对应的保持系B' 4，生产保持系B' 4相对应的不育系S' 1；
    e)对不育系S' 1进行室内外育性鉴定，选留不育性符合不育系特性的有色稻不育系S'，获得有色稻三系不育系S'及其相对应的有色稻保持系B'；
    (2)有色稻恢复系的选育：
    A)以有色稻D为父本，恢复系R为母本，进行杂交，获得的子代与母本进行回交，得到BC mF' 1代，BC mF' 1代自交后，收获BC mF' 2代的种子；
    B)观察BC mF' 2代种子的糙米外观，选有色的种子进行种植，选留收获时子代糙米呈现有色与白色性状分离的恢复系R' 1单株；
    C)考察恢复系R' 1单株的农艺性状，选择其中株高和生育期均一致的恢复系R' 1单株，进行产量、稻米品质及病害抗性的评价，选择糙米呈现有色与白色性状分离的优质、高产和高抗的恢复系R' 2；
    (3)有色稻三系杂交品种新组合的获得：
    I)将步骤e)中获得的有色稻三系不育系S'分别与恢复系R' 2成对测交，获得杂种F 1代；
    II)鉴定所述杂种F 1代的杂种优势，选择具有杂种优势的杂种F 1代， 记为杂种F 1a；并将杂种F 1a所对应的亲本有色稻三系不育系S'和有色稻恢复系R' 3进行复测，待植株成熟时，获得杂种F 1b；观察杂种F 1b的糙米颜色和品质，选择具有五彩色糙米且优质的杂种F 1b所对应恢复系R' 4；
    (4)繁制种生产：将步骤II)获得的有色稻三系不育系S'与恢复系R' 4制种，终选繁制种产量高的恢复系R'；
    (5)建立有色稻三系杂交稻S'/B'×R'、有色稻三系不育系S'、有色稻保持系B'和恢复系R'构成的有色稻三系杂交体系。
  2. 如权利要求1所述的有色稻三系杂交组合的选育方法，其特征在于，所述有色稻C和有色稻D为黑米水稻、红米水稻和紫米水稻中的任意两种。
  3. 如权利要求2所述的有色稻三系杂交组合的选育方法，其特征在于，所述有色稻C为中泽黑珍；所述有色稻D为中泽红珍。
  4. 如权利要求1所述的有色稻三系杂交组合的选育方法，其特征在于，所述三系不育系S、三系保持系B和三系恢复系R为常规优质、高产的三系杂交稻组合。
  5. 如权利要求4所述的有色稻三系杂交组合的选育方法，其特征在于，所述三系不育系S为天丰A；所述三系保持系B为天丰B；所述三系恢复系R为华占；三系杂交稻为天优华占。
  6. 如权利要求1所述的有色稻三系杂交组合的选育方法，其特征在于，步骤a)中，所述n为3～5次；步骤A)中，m为3～5次。
  7. 如权利要求1所述的有色稻三系杂交组合的选育方法，其特征在于，步骤e)中，所述不育系特性为异交结实率≥40％；繁制种产量≥200kg。
  8. 如权利要求1所述的有色稻三系杂交组合的选育方法，其特征在于，步骤C)、II)中，所述优质为稻米品质达国家三级及以上标准；步骤C)中，所述高产为产量≥500kg/亩，所述高抗为抗稻瘟病；步骤II)中，以产量≥500kg/亩，稻米品质达国家三级及以上标准，中抗白叶枯病作为具有杂种优势的评判标准。
PCT/CN2021/074373 2020-03-23 2021-01-29 一种有色稻三系杂交组合的选育方法 WO2021190131A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202010206146.7A CN111264381B (zh) 2020-03-23 2020-03-23 一种有色稻三系杂交组合的选育方法
CN202010206146.7 2020-03-23

Publications (1)

Publication Number Publication Date
WO2021190131A1 true WO2021190131A1 (zh) 2021-09-30

Family

ID=71000752

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2021/074373 WO2021190131A1 (zh) 2020-03-23 2021-01-29 一种有色稻三系杂交组合的选育方法

Country Status (2)

Country Link
CN (1) CN111264381B (zh)
WO (1) WO2021190131A1 (zh)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111264381B (zh) * 2020-03-23 2021-12-17 浙江大学 一种有色稻三系杂交组合的选育方法
CN111264382B (zh) * 2020-03-23 2021-12-17 浙江大学 一种五彩米两系杂交水稻的选育方法
CN113994887B (zh) * 2021-11-19 2022-11-15 浙江大学 一种高观赏性高营养杂交水稻的选育方法
CN113994886B (zh) * 2021-11-19 2022-08-19 浙江大学 一种赏食两用杂交水稻不育系的选育方法
CN115005093A (zh) * 2022-06-14 2022-09-06 防城港市中圆农牧特产种养专业合作社 一种三系杂交水稻组合的培育方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102524052A (zh) * 2012-01-11 2012-07-04 福建农林大学 一种具有产彩色米功能稻的选育方法
CN102715076A (zh) * 2012-07-04 2012-10-10 云南省农业科学院粮食作物研究所 一种五彩稻米及其选育方法
CN102742498A (zh) * 2012-06-18 2012-10-24 芦溪县一村食品有限责任公司 杂交优质紫红米种子培育方法
CN111264382A (zh) * 2020-03-23 2020-06-12 浙江大学 一种五彩米两系杂交水稻的选育方法
CN111264380A (zh) * 2020-03-23 2020-06-12 浙江大学 一种有色稻两系杂交水稻的选育方法
CN111264381A (zh) * 2020-03-23 2020-06-12 浙江大学 一种有色稻三系杂交组合的选育方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103858750B (zh) * 2014-03-24 2016-05-25 浙江绿巨人生物技术有限公司 一种高抗性淀粉杂交粳稻三交组合快速育种的方法
CN103891608B (zh) * 2014-04-15 2016-05-18 浙江杰农生物科技有限公司 一种高锌杂交水稻三交组合快速育种方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102524052A (zh) * 2012-01-11 2012-07-04 福建农林大学 一种具有产彩色米功能稻的选育方法
CN102742498A (zh) * 2012-06-18 2012-10-24 芦溪县一村食品有限责任公司 杂交优质紫红米种子培育方法
CN102715076A (zh) * 2012-07-04 2012-10-10 云南省农业科学院粮食作物研究所 一种五彩稻米及其选育方法
CN111264382A (zh) * 2020-03-23 2020-06-12 浙江大学 一种五彩米两系杂交水稻的选育方法
CN111264380A (zh) * 2020-03-23 2020-06-12 浙江大学 一种有色稻两系杂交水稻的选育方法
CN111264381A (zh) * 2020-03-23 2020-06-12 浙江大学 一种有色稻三系杂交组合的选育方法

Also Published As

Publication number Publication date
CN111264381A (zh) 2020-06-12
CN111264381B (zh) 2021-12-17

Similar Documents

Publication Publication Date Title
WO2021190131A1 (zh) 一种有色稻三系杂交组合的选育方法
CN111264380B (zh) 一种有色稻两系杂交水稻的选育方法
US6414226B1 (en) Inbred tomato line FDR 16-2045
CN101480164B (zh) 一种紫心大白菜新种质的选育方法
CN110089423B (zh) 一种黄叶红米水稻的选育方法
KR20070011238A (ko) 자가수분과 정상 타가수분의 농작물 종군, 품종의 선택육종 방법
WO2021190130A1 (zh) 一种五彩米两系杂交水稻的选育方法
CN112913679B (zh) 一种针对玉米隐性抗病基因的转育方法
CN107581059B (zh) 一种紫色大白菜育种方法
CN103270935A (zh) 一种培育蓝色矮败小麦的方法
CN111328699B (zh) 一种黄色颖壳紫黑色种皮的水稻品种选育方法
CN115968771B (zh) 一种紫甘蓝雄性不育系的选育方法
CN103704125A (zh) 一种利用矮败小麦种质资源培育紫色小麦的方法
CN109618917B (zh) 一种长粒香型抗病优质黑米三系不育系的选育方法
CN104488702B (zh) 紫叶直立穗长穗颈糯两系不育系的选育方法
CN107896976B (zh) 一种特种水稻无融合生殖固定杂种优势的育种方法
CN1726767A (zh) 一种橙色花椰菜的选育方法
CN1954666B (zh) 水稻广保型细胞质雄性不育系及恢复系定向育种方法
CN106035069A (zh) 一种穗部颖壳白化的杂交水稻两系不育系的选育方法
US6284955B1 (en) Corn hybrid P724
CN103766210A (zh) 一种利用太谷核不育小麦种质资源培育紫色小麦的方法
CN114431134B (zh) 一种杂交彩色水稻的选育方法
CN113994887B (zh) 一种高观赏性高营养杂交水稻的选育方法
CN104067929B (zh) 一种三系复粒稻恢复系选育及其复粒杂交稻的创制方法
CN114402993B (zh) 一种彩色优质杂交水稻的选育方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 21776423

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 21776423

Country of ref document: EP

Kind code of ref document: A1