WO2021167269A1 - Composition de blanchiment contenant un extrait de noyau de prune en tant que principe actif - Google Patents

Composition de blanchiment contenant un extrait de noyau de prune en tant que principe actif Download PDF

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WO2021167269A1
WO2021167269A1 PCT/KR2021/001326 KR2021001326W WO2021167269A1 WO 2021167269 A1 WO2021167269 A1 WO 2021167269A1 KR 2021001326 W KR2021001326 W KR 2021001326W WO 2021167269 A1 WO2021167269 A1 WO 2021167269A1
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seed extract
plum
plum seed
skin
composition
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PCT/KR2021/001326
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English (en)
Korean (ko)
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이승욱
조혜린
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계명대학교 산학협력단
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74—Biological properties of particular ingredients
    • A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782—Enzyme inhibitors; Enzyme antagonists

Definitions

  • the present invention relates to a composition for whitening containing a plum seed extract as an active ingredient.
  • Melanin acts to protect the human body by absorbing more than a certain amount of ultraviolet rays and blocking harmful ultraviolet rays from penetrating into the human body.
  • melanin which is the main cause of abnormal skin pigmentation
  • enzymes such as tyrosinase, TRP-1 (tyrosinase related protein-1), TRP-2 using tyrosine as a substrate in cells
  • tyrosinase enzymes such as tyrosinase, TRP-1 (tyrosinase related protein-1), TRP-2 using tyrosine as a substrate in cells
  • Dopaquinone is produced through dopa (3,4-dihydroxyphenylalanine, DOPA), and melanin, the black pigment of the copolymer, is produced through spontaneous reaction and enzymatic reaction from dopaquinone.
  • DOPA dopa (3,4-dihydroxyphenylalanine
  • a method for reducing the production of melanin by inhibiting some reactions in the melanin synthesis process is generally easily performed, and for this purpose, conventionally ascorbic acid, kojic acid ), arbutin, and various plant extracts such as hydroquinone were often used.
  • kojic acid inhibits enzyme activity by chelating copper ions present at the tyrosinase active site. Although it has good performance, there is a problem with safety when formulated in cosmetics. It is highly irritating to the skin, and ascorbic acid also has a safety problem.
  • plum Prunus salicina L.
  • plum is a fruit cultivated since the Silla period in Korea and belongs to the subgenus Prunus in the family Rosaceae.
  • Most of the plums produced in Korea are oriental plums, and 70% of them are produced in Gyeongbuk, and it is one of the main fruits in Korea.
  • Plums are fruits with an early harvest throughout the year, and although the composition differs depending on the variety and environment, it contains a large amount of natural phenolic compounds as useful ingredients. According to a recent study, phenolic compounds are oxidized instead of free radicals and are being studied to be effective in antioxidants, anticancer, antibacterial, etc.
  • Plums are mainly consumed raw or processed into dried plums, jam, nectar, juice, and wine. These processed foods mainly use the flesh and peel, and after manufacturing the processed foods, seeds, which are by-products, are generated and most of them are discarded. Recently, various studies have been conducted to utilize these by-products, but studies on prune by-products for skin whitening or treatment of pigment diseases are still insufficient.
  • An object of the present invention is to provide a composition for skin whitening using a seed extract of discarded fruit.
  • Another object of the present invention is to provide a composition for preventing or treating skin diseases caused by excessive melanin production using a seed extract of discarded fruits.
  • the present invention provides a cosmetic composition for skin whitening containing a plum seed extract as an active ingredient.
  • the present invention provides a health food composition for skin whitening containing a plum seed extract as an active ingredient.
  • the present invention provides a pharmaceutical composition for preventing or treating skin diseases caused by excessive melanin production, comprising a plum seed extract as an active ingredient.
  • the present invention provides a health food composition for preventing or improving skin diseases caused by excessive production of melanin containing the plum seed extract as an active ingredient.
  • the plum seed extract, particularly the pizza bean seed extract, according to the present invention is stable and exhibits superior tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a cosmetic composition for skin whitening, a health food composition, and the like.
  • the plum seed extract as a composition for preventing or treating skin diseases caused by excessive melanin production, abnormal skin pigmentation diseases such as spots, freckles, and age spots caused by excessive melanin production can be effectively prevented or treated.
  • composition according to the present invention uses the seeds of plums, which are by-products of fruits, as an active ingredient, thereby efficiently utilizing wasted resources, thereby helping to protect the environment and save resources.
  • Prunus salicina Formosa is a cross-sectional image of three varieties of plums used in an embodiment of the present invention, Prunus salicina Formosa , Prunus salicina Lindl. cv. Soldam , and Prunus salicina Dodam.
  • FIG. 2 is a flowchart illustrating a method for preparing a plum seed extract according to an embodiment of the present invention.
  • FIG. 3 is a graph confirming the tyrosinase inhibitory activity of the plum seed extract prepared according to FIG. 2 for each plum variety.
  • FIG. 4 is a graph confirming the tyrosinase inhibitory activity according to the concentration of the pizza bean seed extract prepared according to FIG. 2 .
  • FIG. 5 is a graph showing the change in tyrosinase inhibitory activity according to the storage period at room temperature of the pizza bean seed extract prepared according to FIG. 2 .
  • 6 is a graph showing the change in tyrosinase inhibitory activity according to the storage period of ascorbic acid at room temperature.
  • FIG. 9 is a graph showing the results of confirming the melanin content of the four kinds of plum seed extracts of FIG. 7 .
  • the present inventors prepared seed extracts of plums mainly cultivated in Korea, such as hummusa ( Formosa ), pizza bean ( Soldam ), dodam ( Dodam ), Hongrosen ( Hongrosen ) and Daeseok ( Oishiwase ) plums, and their tyrosinase inhibitory activity By confirming etc., this invention was completed.
  • the present invention provides a cosmetic composition for skin whitening containing a plum seed extract as an active ingredient.
  • the "plum seed” is a part of the seed from which the flesh and skin are removed from the plum (Plum). It may be one or more plum seeds selected from the group consisting of, but is not limited thereto.
  • the plum seeds may be obtained from plums harvested or grown in nature, or purchased commercially.
  • extract refers to a material obtained by extracting a component of a natural product regardless of an extraction method, an extraction solvent, an extracted component, or the form of an extract, and the material obtained by extracting the component of a natural product is extracted It may include any material obtainable by processing or processing by the method.
  • the plum seed extract may be extracted with water, C1 to C4 alcohol, or a mixed solvent thereof, preferably 60 to 80% by weight of an aqueous ethanol solution or an aqueous pretanol solution, more preferably 70% by weight It may be a vegetable ethanol extract of pretanol (prethanol).
  • the plum seed extract may be extracted according to a method commonly used in the art, for example, hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc., which are performed alone or in combination of two or more methods can be performed.
  • whitening refers to an action of improving skin troubles by inhibiting, inhibiting, or alleviating excessive deposition of melanin pigment, etc., to prevent blackening of the skin caused by the pigmentation.
  • cosmetics may include functional cosmetics having an effect of preventing or improving skin pigmentation, etc., and the functional cosmetics have specialized functionalities that emphasize physiologically active efficacy and effects, unlike general cosmetics. As defined by the Cosmetics Act, it refers to cosmetics emphasizing specific efficacy and effects such as skin whitening.
  • the plum seed extract may have excellent tyrosinase inhibitory activity.
  • tyrosinase is an enzyme used in the melanin synthesis process, and by inhibiting its activity, it can inhibit melanin production to impart a whitening effect.
  • the plum seed extract was found to have tyrosinase inhibitory activity, and in particular, it was confirmed that the pizza bean and red rosen seed extracts exhibited excellent tyrosinase inhibitory activity.
  • the plum seed extract showed significantly superior stability to tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a more stable and effective cosmetic composition for skin whitening.
  • the plum seed extract may be included in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total cosmetic, but is not limited thereto.
  • the cosmetic composition according to the present invention may further include one or more of the ingredients listed in the list of cosmetic raw materials registered with the Food and Drug Administration and ICID (International Cosmetic Ingredients).
  • the cosmetic composition may include an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a fragrance, a surfactant, an emulsifier, a filler, a sequestering agent, a preservative, a vitamin, a blocking agent, It may further comprise one or more adjuvants commonly used in the cosmetic field, such as wetting agents, dyes and pigments, hydrophilic or lipophilic actives, or any other ingredients commonly used in cosmetics.
  • the vitamin may include a water-soluble or oil-soluble vitamin, and the water-soluble vitamin is a water-soluble vitamin that can be formulated in cosmetics, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, and nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like, and more preferably, vitamin C that helps collagen synthesis.
  • a water-soluble or oil-soluble vitamin is a water-soluble vitamin that can be formulated in cosmetics, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, and nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like, and more preferably, vitamin C that helps collagen synthesis.
  • salts thereof thiamine hydrochloride, sodium ascorbate salt, etc.
  • derivatives ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.
  • the water-soluble vitamin is a microorganism transformation method , can be obtained by a conventional method such as purification from a culture of microorganisms, enzymatic method or chemical synthesis method.
  • the cosmetic composition according to the present invention may be prepared in any formulation conventionally prepared in the technical field to which the present invention pertains.
  • it may be formulated as a lotion, emulsion, lotion, cream, paste, gel, solution, suspension, oil, wax, pack, powder, foundation, spray, surfactant-containing cleansing, etc., but is not limited thereto. .
  • the cosmetic composition according to the present invention is a cream or gel formulation
  • animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be further included as a carrier component.
  • the cosmetic composition is a solution or emulsion formulation
  • a solvent, solvating agent or emulsifying agent water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid It may further include esters and the like.
  • the cosmetic composition is a suspension formulation
  • a liquid diluent such as water, ethanol or propylene glycol
  • a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be further included.
  • lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be further included as a carrier component, and in particular, in the case of a spray formulation, additional chlorofluorohydrocarbon , may further include a propellant such as propane/butane or dimethyl ether.
  • the cosmetic composition is a surfactant-containing cleansing formulation
  • It may further include ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester.
  • the cosmetic composition according to the present invention may be used alone or by being applied in duplicate, or may be used by overlapping with other cosmetic compositions other than the present invention.
  • the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.
  • the present invention provides a health food composition for skin whitening containing a plum seed extract as an active ingredient.
  • the plum seed extract has an excellent tyrosinase inhibitory activity, thereby inhibiting melanin production to impart a whitening effect. According to an experimental example of the present invention, it was confirmed that the plum seed extract had tyrosinase inhibitory activity, and in particular, excellent tyrosinase inhibitory activity was exhibited in the pizza bean and red rosen seed extracts.
  • the plum seed extract showed significantly superior stability to tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a more stable and effective health food composition for skin whitening.
  • the health food may be prepared in powder, granule, tablet, capsule, syrup or beverage, etc. may include all of them.
  • beverages and various drinks, fruits and their processed foods (canned fruit, jam, etc.), fish, meat and their processed foods (ham, bacon, etc.), breads and noodles, cookies and snacks, dairy products (butter, cheese, etc.) ), etc. are possible, and may include all functional foods in a conventional sense. It may also include food used as feed for animals.
  • the health food composition according to the present invention may be prepared by further including pharmaceutically acceptable food additives and other suitable auxiliary ingredients commonly used in the art.
  • suitable auxiliary ingredients commonly used in the art.
  • flavoring agents natural carbohydrates, sweetening agents, vitamins, electrolytes, coloring agents, pectic acid, alginic acid, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents, etc.
  • suitable auxiliary ingredients commonly used in the art.
  • flavoring agents natural carbohydrates, sweetening agents, vitamins, electrolytes, coloring agents, pectic acid, alginic acid, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents, etc.
  • the natural carbohydrate monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol can be used.
  • the sweetener natural sweeteners such as taumatine and stevia extract or synthetic sweeteners such as saccharin and aspartame may be used.
  • the effective dose of the plum seed extract contained in the health food according to the present invention may be appropriately adjusted according to the purpose of skin whitening.
  • the composition has the advantage of not having side effects that may occur during long-term use of general drugs by using natural products and food as raw materials, and has excellent portability and can be taken as an auxiliary for skin whitening.
  • the present invention provides a pharmaceutical composition for preventing or treating skin diseases caused by excessive melanin production, comprising a plum seed extract as an active ingredient.
  • the plum seed extract has excellent tyrosinase inhibitory activity, it is possible to inhibit melanin production, and by using it, it is possible to prevent or treat skin diseases caused by excessive melanin production.
  • the plum seed extract showed significantly superior stability to tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a more stable and effective composition for treating the skin disease.
  • the skin disease caused by the excessive production of melanin may be one or more pigment diseases selected from the group consisting of melasma, freckles, blemishes, age spots and nevus Ota, but is not limited thereto.
  • meltasma is a pigmented disease in which brown spots of irregular shape and various sizes occur on exposed areas, especially on the face, and may be exacerbated by exposure to sunlight or the like. Melanin pigment is deposited mainly on the cheeks, forehead, and under the eyes symmetrically.
  • “freckle” is a small, yellowish-brown, pigmented spot that occurs mainly on the skin in areas exposed to sunlight, the cause of which is unknown, but melanocortin-1-receptor (melanocortin-1-receptor) It is related to the genetic mutation of , and it is known that it can occur because the synthesis of melanin pigment is increased by stimulation of skin melanocytes by ultraviolet rays.
  • live spot, age spot is a stain on the skin formed by aging and exposure to ultraviolet rays of sunlight, and is also referred to as sun melanoma (sunlight spot, solar lentigo) or ganban.
  • nevus of Ota refers to a spot having a brown or blue hue that occurs well on the skin, eyes, and mucous membranes around the eyes.
  • the pharmaceutical composition according to the present invention may be prepared according to a conventional method in the pharmaceutical field.
  • the pharmaceutical composition may be combined with a suitable pharmaceutically acceptable carrier according to the formulation, and if necessary, excipients, diluents, dispersants, emulsifiers, buffers, stabilizers, binders, disintegrants, solvents, etc. may be prepared further comprising have.
  • the appropriate carrier and the like do not inhibit the activity or properties of the plum seed extract according to the present invention, and may be selected differently depending on the dosage form and formulation.
  • the pharmaceutical composition according to the present invention can be applied in any dosage form, and more specifically, it can be used by formulating oral dosage forms, external preparations, suppositories, and parenteral dosage forms of sterile injection solutions according to conventional methods.
  • the solid dosage form among the oral dosage forms is in the form of tablets, pills, powders, granules, capsules, etc., and at least one or more excipients, for example, starch, calcium carbonate, sucrose, lactose, sorbitol, mannitol, cellulose, gelatin, etc.
  • excipients for example, starch, calcium carbonate, sucrose, lactose, sorbitol, mannitol, cellulose, gelatin, etc.
  • lubricants such as magnesium stearate and talc may be included.
  • the capsule formulation may further include a liquid carrier such as fatty oil in addition to the above-mentioned substances.
  • liquid formulations include suspensions, solutions, emulsions, syrups, etc.
  • various excipients for example, wetting agents, sweeteners, fragrances, preservatives, etc. may be included. have.
  • the parenteral formulation may include a sterile aqueous solution, a non-aqueous solution, a suspension, an emulsion, a freeze-dried formulation, and a suppository.
  • Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate.
  • As the base of the suppository witepsol, macrogol, Tween 61, cacao butter, laurin fat, glycerogelatin, and the like can be used. Without being limited thereto, any suitable agent known in the art may be used.
  • composition according to the present invention may further add calcium, vitamin D3, or the like to enhance therapeutic efficacy.
  • the pharmaceutical composition according to the present invention may be administered in a pharmaceutically effective amount.
  • pharmaceutically effective amount means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment and not to cause side effects.
  • the effective dose level of the pharmaceutical composition is determined by the purpose of use, the age, sex, weight and health status of the patient, the type of disease, severity, drug activity, sensitivity to drug, administration method, administration time, administration route and excretion rate, treatment It may be determined differently depending on factors including the duration, formulation or concurrent use of drugs and other factors well known in the medical field. For example, although not constant, generally 0.001 to 100 mg/kg, preferably 0.01 to 10 mg/kg, may be administered once to several times a day. The above dosage does not limit the scope of the present invention in any way.
  • the pharmaceutical composition according to the present invention may be administered by an appropriate administration route according to the form of the formulation, and may be administered through various routes, either oral or parenteral, as long as it can reach the target tissue.
  • the administration method does not need to be particularly limited, and for example, oral, rectal or intravenous, muscle, skin application, subcutaneous, respiratory inhalation, intrauterine dural or intracerebroventricular injection, etc. are administered in a conventional manner. can be
  • the pharmaceutical composition according to the present invention may be used alone for the prevention or treatment of skin diseases caused by excessive melanin production, or may be used in combination with surgery or other drug treatment.
  • the present invention provides a health food composition for preventing or improving skin diseases caused by excessive production of melanin containing the plum seed extract as an active ingredient.
  • the present invention may provide a reagent composition for inhibiting the activity of tyrosinase containing a plum seed extract as an active ingredient.
  • Example 1 Preparation of plum seed (seed) extracts for each variety of Humusa, Pizza Doo, and Dodam.
  • the plums used in this experiment are Prunus salicina Formosa , Prunus salicina Lindl. cv. Soldam , and Prunus salicina Dodam 3 types, respectively, from Gimcheon, Yeongcheon, and Gyeongsan.
  • the seeds were completely isolated from the pulp, purchased online and at a fruit store, and only the seeds were used for the experiment.
  • pretanol A prethanol A, Duksan, Ansan, Gyeonggi-do, Korea
  • v/w ethanol for food addition at 10 times (v/w) of the weight of the powder
  • Extraction was repeated twice for 18 hours each.
  • the extract was filtered through filter paper (Whatman No.3, England), and the filtrate was concentrated with a rotary vacuum concentrator on a 45°C water bath, then freeze-dried, stored at -20°C, and used in the experiment.
  • FIG. 2 A detailed manufacturing process is shown in FIG. 2 .
  • Tyrosinase inhibitory activity was measured in a mixture of 80 ⁇ L of 0.1 M potassium phosphate buffer (pH 7.0), 40 ⁇ L of 0.01 M L-Dopa and 40 ⁇ L of sample solution, and 125 U/mL of mushroom tyrosinase. was added and reacted at 37° C. for 3 minutes, and the dopachrome generated in the reaction solution was measured at 475 nm.
  • the pizza bean seed extract showed the highest tyrosinase inhibitory activity.
  • the concentration-dependent high inhibitory activity was shown in the 0.25-1.5 mg/mL concentration range.
  • ascorbic acid which is known as a representative whitening raw material, decreased about 78% in tyrosinase inhibitory activity compared to the first day at a concentration of 25 ⁇ g/mL on the 8th day, as shown in FIG. 6, and it was confirmed that the inhibitory activity was reduced by nearly 90% at all concentrations on the 20th could
  • a plum seed extract of Daeseok (Oishiwase ) and Hongrosen (Hongrosen) varieties was additionally prepared by the method according to Example 1.
  • B16-F10 cells used in the experiment were purchased from the Korea Cell Line Bank and used. Cells were prepared using Dulbecco's modified Eagle's medium (DMEM) medium supplemented with 10% fetal bovine serum, 100unit/mL penicillin, and 100mg/mL streptomycin at 5% CO 2 and 37°C. It was used while subcultured under the conditions.
  • DMEM Dulbecco's modified Eagle's medium
  • B16-F10 cells were aliquoted into 48-wells so as to become 0.5 ⁇ 10 4 cells/well and cultured for 24 hours. After removing the culture medium and treating the four varieties of plum seed extracts by concentration, incubated for 68 hours in an incubator at 5% CO 2 , 37° C., 20 ⁇ L of MTT solution prepared at a concentration of 2.5 mg/mL was treated with 4 time incubation. After that, the culture medium was removed and dimethyl sulfoxide (dimethyl sulfoxide, DMSO) was added to dissolve the MTT-formazan crystals generated in living cells, and absorbance was measured at 550 nm.
  • DMSO dimethyl sulfoxide
  • B16-F10 cells were first aliquoted into 48-wells at a volume of 0.5 ⁇ 10 4 cells/well, cultured for 24 hours, and then treated with plum seed extracts for each variety. After 1 hour of treatment with the extract, 1 ⁇ M ⁇ -melanocyte stimulating hormone ( ⁇ -MSH) was treated and incubated for 72 hours, the culture medium was removed and washed with PBS (phosphate buffered saline). Add 100 ⁇ L of reporter lysis buffer to lyse the cells, centrifuge at 1,000 rpm, 4° C. for 5 minutes, collect the supernatant, add 100 ⁇ L of 10 mM L-DOPA to 40 ⁇ L of the supernatant, react at 37° C. for 2 hours, and then at 475 nm. Absorbance was measured.
  • ⁇ -MSH ⁇ M ⁇ -melanocyte stimulating hormone
  • B16-F10 cells were aliquoted into 6-wells so that 0.5 ⁇ 10 4 cells/well were cultured for 24 hours, and then the plum seed extracts for each variety were treated. After 1 hour, 1 ⁇ M ⁇ -MSH was treated, cultured for 72 hours, the culture medium was removed and washed with PBS. Then, 100 ⁇ L of PBS was dispensed, centrifuged at 5,000 rpm, 4° C. for 5 minutes, the supernatant was discarded, and 400 ⁇ L of 1N NaOH solution containing 10% DMSO was added to the pellet each, dissolved at 80° C. for 1 hour, and then dissolved at 405 nm. Absorbance was measured.
  • SPSS Statistics 25 (Statistical Package for the Social Science, Version 25.0 SPSS Inc., Chicago, IL, USA) was used. All data were repeated three or more times and expressed as the mean and standard error (mean ⁇ SEM). One-way ANOVA was used to verify the difference in significance between groups, and a post-hoc test using Duncan's multiple range test was performed. In addition, significant differences in each interval were verified at a significance level of 95% (P ⁇ 0.05).
  • MTT assay 3-(4,5-dimethylthiazolyl-2)-2,5- Diphenyl tetrazolium bromide [3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide, MTT] assay (MTT assay) was performed.
  • MTT assay is a test method to measure the amount of purple MTT formazan produced by the reaction of mitochondrial dehydrogenase with yellow MTT tetrazolium, and the absorbance is maximized at a wavelength near 550 nm. This is one of the methods widely used as a cytotoxicity and cell proliferation detection method because many samples can be read simply.
  • each sample was treated at a concentration of 200-500 ⁇ g/mL, and then MTT assay was performed.
  • MTT assay was performed at 400 ⁇ g/mL of plum seed extract, 73% of Daeseok, 79% of Humusa, 70% of red rosen, and 70% of pizza bean showed survival rates. Therefore, subsequent experiments were carried out at a concentration of 400 ⁇ g/mL with a cytotoxicity of 30% or less.
  • ⁇ -MSH is a melanogenesis inducer, secreted from various peripheral tissues including the pituitary gland and skin, and is involved in various physiological functions such as melanin production, epidermal cell growth and proliferation, and local tissue immune regulation.
  • B16-F10 cells were treated with 400 ⁇ g/mL of plum seed extract and 1 ⁇ M of ⁇ -MSH, and for comparison, untreated group (control), ⁇ -MSH alone treatment
  • group 100 ⁇ g/mL arbutin, as shown in FIG. 8
  • Musa and pizzadu showed 98%, 77%, and 50% tyrosinase inhibitory activity, respectively, in that order.
  • Daeseok it did not show significant inhibitory activity.
  • red rosen was superior, which means that the tyrosinase inhibitory activity of red rosen itself, not the activity caused by cytotoxicity, may be relatively superior.
  • B16-F10 cells were treated with the plum seed extracts of each variety and ⁇ -MSH, and for comparison, the untreated group (control), ⁇ -MSH alone treatment group, 100 ⁇ g / mL arbutin treatment group and the experiment was conducted to investigate the melanin content, as shown in FIG.
  • arbutin a positive control, showed 46% inhibitory activity, and there was no significant difference compared with red rosen and pizza bean, and the effects on melanin synthesis and tyrosinase activity all showed similar trends in the four extracts.

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Abstract

La présente invention concerne une composition de blanchiment comprenant un extrait de noyau de prune en tant que principe actif, et plus spécifiquement, fournit une composition cosmétique de blanchiment de la peau, une composition d'aliment diététique, et une composition pour prévenir ou traiter des affections cutanées provoquées par une surproduction de mélanine, chacune d'entre elles comprenant un extrait de noyau de prune en tant que principe actif. L'extrait de noyau de prune selon la présente invention présente une activité d'inhibition de la tyrosinase plus stable et supérieure par rapport à l'acide ascorbique classiquement utilisé comme matériau de blanchiment, et peut ainsi être utilisé en tant que matière cosmétique fonctionnelle pour le blanchiment de la peau et peut efficacement prévenir ou traiter des troubles anormaux de pigmentation de la peau tels que les taches brunes, les taches de rousseur et les taches solaires.
PCT/KR2021/001326 2020-02-19 2021-02-02 Composition de blanchiment contenant un extrait de noyau de prune en tant que principe actif WO2021167269A1 (fr)

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JPH10236969A (ja) * 1997-02-24 1998-09-08 Nonogawa Shoji Kk 抗ストレス及び紫外線障害防御剤
KR20180093560A (ko) * 2017-02-14 2018-08-22 (주)튜링겐코리아 적과자두 추출물을 포함하는 항산화, 피부미백, 수렴 또는 주름 개선을 위한 조성물

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KR20120036516A (ko) 2010-10-08 2012-04-18 주식회사 바이오랜드 브로콜리 종자 추출물을 함유하는 피부미백용 화장료 조성물
KR102015176B1 (ko) * 2017-09-27 2019-08-27 대구한의대학교산학협력단 황매실 종자 에센셜 오일 및 이를 포함하는 미백 화장료 조성물

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KR20180093560A (ko) * 2017-02-14 2018-08-22 (주)튜링겐코리아 적과자두 추출물을 포함하는 항산화, 피부미백, 수렴 또는 주름 개선을 위한 조성물

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