WO2021167269A1 - Whitening composition comprising plum seed extract as active ingredient - Google Patents

Whitening composition comprising plum seed extract as active ingredient Download PDF

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Publication number
WO2021167269A1
WO2021167269A1 PCT/KR2021/001326 KR2021001326W WO2021167269A1 WO 2021167269 A1 WO2021167269 A1 WO 2021167269A1 KR 2021001326 W KR2021001326 W KR 2021001326W WO 2021167269 A1 WO2021167269 A1 WO 2021167269A1
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Prior art keywords
seed extract
plum
plum seed
skin
composition
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PCT/KR2021/001326
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French (fr)
Korean (ko)
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이승욱
조혜린
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계명대학교 산학협력단
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Publication of WO2021167269A1 publication Critical patent/WO2021167269A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74—Biological properties of particular ingredients
    • A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782—Enzyme inhibitors; Enzyme antagonists

Definitions

  • the present invention relates to a composition for whitening containing a plum seed extract as an active ingredient.
  • Melanin acts to protect the human body by absorbing more than a certain amount of ultraviolet rays and blocking harmful ultraviolet rays from penetrating into the human body.
  • melanin which is the main cause of abnormal skin pigmentation
  • enzymes such as tyrosinase, TRP-1 (tyrosinase related protein-1), TRP-2 using tyrosine as a substrate in cells
  • tyrosinase enzymes such as tyrosinase, TRP-1 (tyrosinase related protein-1), TRP-2 using tyrosine as a substrate in cells
  • Dopaquinone is produced through dopa (3,4-dihydroxyphenylalanine, DOPA), and melanin, the black pigment of the copolymer, is produced through spontaneous reaction and enzymatic reaction from dopaquinone.
  • DOPA dopa (3,4-dihydroxyphenylalanine
  • a method for reducing the production of melanin by inhibiting some reactions in the melanin synthesis process is generally easily performed, and for this purpose, conventionally ascorbic acid, kojic acid ), arbutin, and various plant extracts such as hydroquinone were often used.
  • kojic acid inhibits enzyme activity by chelating copper ions present at the tyrosinase active site. Although it has good performance, there is a problem with safety when formulated in cosmetics. It is highly irritating to the skin, and ascorbic acid also has a safety problem.
  • plum Prunus salicina L.
  • plum is a fruit cultivated since the Silla period in Korea and belongs to the subgenus Prunus in the family Rosaceae.
  • Most of the plums produced in Korea are oriental plums, and 70% of them are produced in Gyeongbuk, and it is one of the main fruits in Korea.
  • Plums are fruits with an early harvest throughout the year, and although the composition differs depending on the variety and environment, it contains a large amount of natural phenolic compounds as useful ingredients. According to a recent study, phenolic compounds are oxidized instead of free radicals and are being studied to be effective in antioxidants, anticancer, antibacterial, etc.
  • Plums are mainly consumed raw or processed into dried plums, jam, nectar, juice, and wine. These processed foods mainly use the flesh and peel, and after manufacturing the processed foods, seeds, which are by-products, are generated and most of them are discarded. Recently, various studies have been conducted to utilize these by-products, but studies on prune by-products for skin whitening or treatment of pigment diseases are still insufficient.
  • An object of the present invention is to provide a composition for skin whitening using a seed extract of discarded fruit.
  • Another object of the present invention is to provide a composition for preventing or treating skin diseases caused by excessive melanin production using a seed extract of discarded fruits.
  • the present invention provides a cosmetic composition for skin whitening containing a plum seed extract as an active ingredient.
  • the present invention provides a health food composition for skin whitening containing a plum seed extract as an active ingredient.
  • the present invention provides a pharmaceutical composition for preventing or treating skin diseases caused by excessive melanin production, comprising a plum seed extract as an active ingredient.
  • the present invention provides a health food composition for preventing or improving skin diseases caused by excessive production of melanin containing the plum seed extract as an active ingredient.
  • the plum seed extract, particularly the pizza bean seed extract, according to the present invention is stable and exhibits superior tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a cosmetic composition for skin whitening, a health food composition, and the like.
  • the plum seed extract as a composition for preventing or treating skin diseases caused by excessive melanin production, abnormal skin pigmentation diseases such as spots, freckles, and age spots caused by excessive melanin production can be effectively prevented or treated.
  • composition according to the present invention uses the seeds of plums, which are by-products of fruits, as an active ingredient, thereby efficiently utilizing wasted resources, thereby helping to protect the environment and save resources.
  • Prunus salicina Formosa is a cross-sectional image of three varieties of plums used in an embodiment of the present invention, Prunus salicina Formosa , Prunus salicina Lindl. cv. Soldam , and Prunus salicina Dodam.
  • FIG. 2 is a flowchart illustrating a method for preparing a plum seed extract according to an embodiment of the present invention.
  • FIG. 3 is a graph confirming the tyrosinase inhibitory activity of the plum seed extract prepared according to FIG. 2 for each plum variety.
  • FIG. 4 is a graph confirming the tyrosinase inhibitory activity according to the concentration of the pizza bean seed extract prepared according to FIG. 2 .
  • FIG. 5 is a graph showing the change in tyrosinase inhibitory activity according to the storage period at room temperature of the pizza bean seed extract prepared according to FIG. 2 .
  • 6 is a graph showing the change in tyrosinase inhibitory activity according to the storage period of ascorbic acid at room temperature.
  • FIG. 9 is a graph showing the results of confirming the melanin content of the four kinds of plum seed extracts of FIG. 7 .
  • the present inventors prepared seed extracts of plums mainly cultivated in Korea, such as hummusa ( Formosa ), pizza bean ( Soldam ), dodam ( Dodam ), Hongrosen ( Hongrosen ) and Daeseok ( Oishiwase ) plums, and their tyrosinase inhibitory activity By confirming etc., this invention was completed.
  • the present invention provides a cosmetic composition for skin whitening containing a plum seed extract as an active ingredient.
  • the "plum seed” is a part of the seed from which the flesh and skin are removed from the plum (Plum). It may be one or more plum seeds selected from the group consisting of, but is not limited thereto.
  • the plum seeds may be obtained from plums harvested or grown in nature, or purchased commercially.
  • extract refers to a material obtained by extracting a component of a natural product regardless of an extraction method, an extraction solvent, an extracted component, or the form of an extract, and the material obtained by extracting the component of a natural product is extracted It may include any material obtainable by processing or processing by the method.
  • the plum seed extract may be extracted with water, C1 to C4 alcohol, or a mixed solvent thereof, preferably 60 to 80% by weight of an aqueous ethanol solution or an aqueous pretanol solution, more preferably 70% by weight It may be a vegetable ethanol extract of pretanol (prethanol).
  • the plum seed extract may be extracted according to a method commonly used in the art, for example, hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc., which are performed alone or in combination of two or more methods can be performed.
  • whitening refers to an action of improving skin troubles by inhibiting, inhibiting, or alleviating excessive deposition of melanin pigment, etc., to prevent blackening of the skin caused by the pigmentation.
  • cosmetics may include functional cosmetics having an effect of preventing or improving skin pigmentation, etc., and the functional cosmetics have specialized functionalities that emphasize physiologically active efficacy and effects, unlike general cosmetics. As defined by the Cosmetics Act, it refers to cosmetics emphasizing specific efficacy and effects such as skin whitening.
  • the plum seed extract may have excellent tyrosinase inhibitory activity.
  • tyrosinase is an enzyme used in the melanin synthesis process, and by inhibiting its activity, it can inhibit melanin production to impart a whitening effect.
  • the plum seed extract was found to have tyrosinase inhibitory activity, and in particular, it was confirmed that the pizza bean and red rosen seed extracts exhibited excellent tyrosinase inhibitory activity.
  • the plum seed extract showed significantly superior stability to tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a more stable and effective cosmetic composition for skin whitening.
  • the plum seed extract may be included in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total cosmetic, but is not limited thereto.
  • the cosmetic composition according to the present invention may further include one or more of the ingredients listed in the list of cosmetic raw materials registered with the Food and Drug Administration and ICID (International Cosmetic Ingredients).
  • the cosmetic composition may include an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a fragrance, a surfactant, an emulsifier, a filler, a sequestering agent, a preservative, a vitamin, a blocking agent, It may further comprise one or more adjuvants commonly used in the cosmetic field, such as wetting agents, dyes and pigments, hydrophilic or lipophilic actives, or any other ingredients commonly used in cosmetics.
  • the vitamin may include a water-soluble or oil-soluble vitamin, and the water-soluble vitamin is a water-soluble vitamin that can be formulated in cosmetics, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, and nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like, and more preferably, vitamin C that helps collagen synthesis.
  • a water-soluble or oil-soluble vitamin is a water-soluble vitamin that can be formulated in cosmetics, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, and nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like, and more preferably, vitamin C that helps collagen synthesis.
  • salts thereof thiamine hydrochloride, sodium ascorbate salt, etc.
  • derivatives ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.
  • the water-soluble vitamin is a microorganism transformation method , can be obtained by a conventional method such as purification from a culture of microorganisms, enzymatic method or chemical synthesis method.
  • the cosmetic composition according to the present invention may be prepared in any formulation conventionally prepared in the technical field to which the present invention pertains.
  • it may be formulated as a lotion, emulsion, lotion, cream, paste, gel, solution, suspension, oil, wax, pack, powder, foundation, spray, surfactant-containing cleansing, etc., but is not limited thereto. .
  • the cosmetic composition according to the present invention is a cream or gel formulation
  • animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be further included as a carrier component.
  • the cosmetic composition is a solution or emulsion formulation
  • a solvent, solvating agent or emulsifying agent water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid It may further include esters and the like.
  • the cosmetic composition is a suspension formulation
  • a liquid diluent such as water, ethanol or propylene glycol
  • a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be further included.
  • lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be further included as a carrier component, and in particular, in the case of a spray formulation, additional chlorofluorohydrocarbon , may further include a propellant such as propane/butane or dimethyl ether.
  • the cosmetic composition is a surfactant-containing cleansing formulation
  • It may further include ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester.
  • the cosmetic composition according to the present invention may be used alone or by being applied in duplicate, or may be used by overlapping with other cosmetic compositions other than the present invention.
  • the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.
  • the present invention provides a health food composition for skin whitening containing a plum seed extract as an active ingredient.
  • the plum seed extract has an excellent tyrosinase inhibitory activity, thereby inhibiting melanin production to impart a whitening effect. According to an experimental example of the present invention, it was confirmed that the plum seed extract had tyrosinase inhibitory activity, and in particular, excellent tyrosinase inhibitory activity was exhibited in the pizza bean and red rosen seed extracts.
  • the plum seed extract showed significantly superior stability to tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a more stable and effective health food composition for skin whitening.
  • the health food may be prepared in powder, granule, tablet, capsule, syrup or beverage, etc. may include all of them.
  • beverages and various drinks, fruits and their processed foods (canned fruit, jam, etc.), fish, meat and their processed foods (ham, bacon, etc.), breads and noodles, cookies and snacks, dairy products (butter, cheese, etc.) ), etc. are possible, and may include all functional foods in a conventional sense. It may also include food used as feed for animals.
  • the health food composition according to the present invention may be prepared by further including pharmaceutically acceptable food additives and other suitable auxiliary ingredients commonly used in the art.
  • suitable auxiliary ingredients commonly used in the art.
  • flavoring agents natural carbohydrates, sweetening agents, vitamins, electrolytes, coloring agents, pectic acid, alginic acid, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents, etc.
  • suitable auxiliary ingredients commonly used in the art.
  • flavoring agents natural carbohydrates, sweetening agents, vitamins, electrolytes, coloring agents, pectic acid, alginic acid, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents, etc.
  • the natural carbohydrate monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol can be used.
  • the sweetener natural sweeteners such as taumatine and stevia extract or synthetic sweeteners such as saccharin and aspartame may be used.
  • the effective dose of the plum seed extract contained in the health food according to the present invention may be appropriately adjusted according to the purpose of skin whitening.
  • the composition has the advantage of not having side effects that may occur during long-term use of general drugs by using natural products and food as raw materials, and has excellent portability and can be taken as an auxiliary for skin whitening.
  • the present invention provides a pharmaceutical composition for preventing or treating skin diseases caused by excessive melanin production, comprising a plum seed extract as an active ingredient.
  • the plum seed extract has excellent tyrosinase inhibitory activity, it is possible to inhibit melanin production, and by using it, it is possible to prevent or treat skin diseases caused by excessive melanin production.
  • the plum seed extract showed significantly superior stability to tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a more stable and effective composition for treating the skin disease.
  • the skin disease caused by the excessive production of melanin may be one or more pigment diseases selected from the group consisting of melasma, freckles, blemishes, age spots and nevus Ota, but is not limited thereto.
  • meltasma is a pigmented disease in which brown spots of irregular shape and various sizes occur on exposed areas, especially on the face, and may be exacerbated by exposure to sunlight or the like. Melanin pigment is deposited mainly on the cheeks, forehead, and under the eyes symmetrically.
  • “freckle” is a small, yellowish-brown, pigmented spot that occurs mainly on the skin in areas exposed to sunlight, the cause of which is unknown, but melanocortin-1-receptor (melanocortin-1-receptor) It is related to the genetic mutation of , and it is known that it can occur because the synthesis of melanin pigment is increased by stimulation of skin melanocytes by ultraviolet rays.
  • live spot, age spot is a stain on the skin formed by aging and exposure to ultraviolet rays of sunlight, and is also referred to as sun melanoma (sunlight spot, solar lentigo) or ganban.
  • nevus of Ota refers to a spot having a brown or blue hue that occurs well on the skin, eyes, and mucous membranes around the eyes.
  • the pharmaceutical composition according to the present invention may be prepared according to a conventional method in the pharmaceutical field.
  • the pharmaceutical composition may be combined with a suitable pharmaceutically acceptable carrier according to the formulation, and if necessary, excipients, diluents, dispersants, emulsifiers, buffers, stabilizers, binders, disintegrants, solvents, etc. may be prepared further comprising have.
  • the appropriate carrier and the like do not inhibit the activity or properties of the plum seed extract according to the present invention, and may be selected differently depending on the dosage form and formulation.
  • the pharmaceutical composition according to the present invention can be applied in any dosage form, and more specifically, it can be used by formulating oral dosage forms, external preparations, suppositories, and parenteral dosage forms of sterile injection solutions according to conventional methods.
  • the solid dosage form among the oral dosage forms is in the form of tablets, pills, powders, granules, capsules, etc., and at least one or more excipients, for example, starch, calcium carbonate, sucrose, lactose, sorbitol, mannitol, cellulose, gelatin, etc.
  • excipients for example, starch, calcium carbonate, sucrose, lactose, sorbitol, mannitol, cellulose, gelatin, etc.
  • lubricants such as magnesium stearate and talc may be included.
  • the capsule formulation may further include a liquid carrier such as fatty oil in addition to the above-mentioned substances.
  • liquid formulations include suspensions, solutions, emulsions, syrups, etc.
  • various excipients for example, wetting agents, sweeteners, fragrances, preservatives, etc. may be included. have.
  • the parenteral formulation may include a sterile aqueous solution, a non-aqueous solution, a suspension, an emulsion, a freeze-dried formulation, and a suppository.
  • Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate.
  • As the base of the suppository witepsol, macrogol, Tween 61, cacao butter, laurin fat, glycerogelatin, and the like can be used. Without being limited thereto, any suitable agent known in the art may be used.
  • composition according to the present invention may further add calcium, vitamin D3, or the like to enhance therapeutic efficacy.
  • the pharmaceutical composition according to the present invention may be administered in a pharmaceutically effective amount.
  • pharmaceutically effective amount means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment and not to cause side effects.
  • the effective dose level of the pharmaceutical composition is determined by the purpose of use, the age, sex, weight and health status of the patient, the type of disease, severity, drug activity, sensitivity to drug, administration method, administration time, administration route and excretion rate, treatment It may be determined differently depending on factors including the duration, formulation or concurrent use of drugs and other factors well known in the medical field. For example, although not constant, generally 0.001 to 100 mg/kg, preferably 0.01 to 10 mg/kg, may be administered once to several times a day. The above dosage does not limit the scope of the present invention in any way.
  • the pharmaceutical composition according to the present invention may be administered by an appropriate administration route according to the form of the formulation, and may be administered through various routes, either oral or parenteral, as long as it can reach the target tissue.
  • the administration method does not need to be particularly limited, and for example, oral, rectal or intravenous, muscle, skin application, subcutaneous, respiratory inhalation, intrauterine dural or intracerebroventricular injection, etc. are administered in a conventional manner. can be
  • the pharmaceutical composition according to the present invention may be used alone for the prevention or treatment of skin diseases caused by excessive melanin production, or may be used in combination with surgery or other drug treatment.
  • the present invention provides a health food composition for preventing or improving skin diseases caused by excessive production of melanin containing the plum seed extract as an active ingredient.
  • the present invention may provide a reagent composition for inhibiting the activity of tyrosinase containing a plum seed extract as an active ingredient.
  • Example 1 Preparation of plum seed (seed) extracts for each variety of Humusa, Pizza Doo, and Dodam.
  • the plums used in this experiment are Prunus salicina Formosa , Prunus salicina Lindl. cv. Soldam , and Prunus salicina Dodam 3 types, respectively, from Gimcheon, Yeongcheon, and Gyeongsan.
  • the seeds were completely isolated from the pulp, purchased online and at a fruit store, and only the seeds were used for the experiment.
  • pretanol A prethanol A, Duksan, Ansan, Gyeonggi-do, Korea
  • v/w ethanol for food addition at 10 times (v/w) of the weight of the powder
  • Extraction was repeated twice for 18 hours each.
  • the extract was filtered through filter paper (Whatman No.3, England), and the filtrate was concentrated with a rotary vacuum concentrator on a 45°C water bath, then freeze-dried, stored at -20°C, and used in the experiment.
  • FIG. 2 A detailed manufacturing process is shown in FIG. 2 .
  • Tyrosinase inhibitory activity was measured in a mixture of 80 ⁇ L of 0.1 M potassium phosphate buffer (pH 7.0), 40 ⁇ L of 0.01 M L-Dopa and 40 ⁇ L of sample solution, and 125 U/mL of mushroom tyrosinase. was added and reacted at 37° C. for 3 minutes, and the dopachrome generated in the reaction solution was measured at 475 nm.
  • the pizza bean seed extract showed the highest tyrosinase inhibitory activity.
  • the concentration-dependent high inhibitory activity was shown in the 0.25-1.5 mg/mL concentration range.
  • ascorbic acid which is known as a representative whitening raw material, decreased about 78% in tyrosinase inhibitory activity compared to the first day at a concentration of 25 ⁇ g/mL on the 8th day, as shown in FIG. 6, and it was confirmed that the inhibitory activity was reduced by nearly 90% at all concentrations on the 20th could
  • a plum seed extract of Daeseok (Oishiwase ) and Hongrosen (Hongrosen) varieties was additionally prepared by the method according to Example 1.
  • B16-F10 cells used in the experiment were purchased from the Korea Cell Line Bank and used. Cells were prepared using Dulbecco's modified Eagle's medium (DMEM) medium supplemented with 10% fetal bovine serum, 100unit/mL penicillin, and 100mg/mL streptomycin at 5% CO 2 and 37°C. It was used while subcultured under the conditions.
  • DMEM Dulbecco's modified Eagle's medium
  • B16-F10 cells were aliquoted into 48-wells so as to become 0.5 ⁇ 10 4 cells/well and cultured for 24 hours. After removing the culture medium and treating the four varieties of plum seed extracts by concentration, incubated for 68 hours in an incubator at 5% CO 2 , 37° C., 20 ⁇ L of MTT solution prepared at a concentration of 2.5 mg/mL was treated with 4 time incubation. After that, the culture medium was removed and dimethyl sulfoxide (dimethyl sulfoxide, DMSO) was added to dissolve the MTT-formazan crystals generated in living cells, and absorbance was measured at 550 nm.
  • DMSO dimethyl sulfoxide
  • B16-F10 cells were first aliquoted into 48-wells at a volume of 0.5 ⁇ 10 4 cells/well, cultured for 24 hours, and then treated with plum seed extracts for each variety. After 1 hour of treatment with the extract, 1 ⁇ M ⁇ -melanocyte stimulating hormone ( ⁇ -MSH) was treated and incubated for 72 hours, the culture medium was removed and washed with PBS (phosphate buffered saline). Add 100 ⁇ L of reporter lysis buffer to lyse the cells, centrifuge at 1,000 rpm, 4° C. for 5 minutes, collect the supernatant, add 100 ⁇ L of 10 mM L-DOPA to 40 ⁇ L of the supernatant, react at 37° C. for 2 hours, and then at 475 nm. Absorbance was measured.
  • ⁇ -MSH ⁇ M ⁇ -melanocyte stimulating hormone
  • B16-F10 cells were aliquoted into 6-wells so that 0.5 ⁇ 10 4 cells/well were cultured for 24 hours, and then the plum seed extracts for each variety were treated. After 1 hour, 1 ⁇ M ⁇ -MSH was treated, cultured for 72 hours, the culture medium was removed and washed with PBS. Then, 100 ⁇ L of PBS was dispensed, centrifuged at 5,000 rpm, 4° C. for 5 minutes, the supernatant was discarded, and 400 ⁇ L of 1N NaOH solution containing 10% DMSO was added to the pellet each, dissolved at 80° C. for 1 hour, and then dissolved at 405 nm. Absorbance was measured.
  • SPSS Statistics 25 (Statistical Package for the Social Science, Version 25.0 SPSS Inc., Chicago, IL, USA) was used. All data were repeated three or more times and expressed as the mean and standard error (mean ⁇ SEM). One-way ANOVA was used to verify the difference in significance between groups, and a post-hoc test using Duncan's multiple range test was performed. In addition, significant differences in each interval were verified at a significance level of 95% (P ⁇ 0.05).
  • MTT assay 3-(4,5-dimethylthiazolyl-2)-2,5- Diphenyl tetrazolium bromide [3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide, MTT] assay (MTT assay) was performed.
  • MTT assay is a test method to measure the amount of purple MTT formazan produced by the reaction of mitochondrial dehydrogenase with yellow MTT tetrazolium, and the absorbance is maximized at a wavelength near 550 nm. This is one of the methods widely used as a cytotoxicity and cell proliferation detection method because many samples can be read simply.
  • each sample was treated at a concentration of 200-500 ⁇ g/mL, and then MTT assay was performed.
  • MTT assay was performed at 400 ⁇ g/mL of plum seed extract, 73% of Daeseok, 79% of Humusa, 70% of red rosen, and 70% of pizza bean showed survival rates. Therefore, subsequent experiments were carried out at a concentration of 400 ⁇ g/mL with a cytotoxicity of 30% or less.
  • ⁇ -MSH is a melanogenesis inducer, secreted from various peripheral tissues including the pituitary gland and skin, and is involved in various physiological functions such as melanin production, epidermal cell growth and proliferation, and local tissue immune regulation.
  • B16-F10 cells were treated with 400 ⁇ g/mL of plum seed extract and 1 ⁇ M of ⁇ -MSH, and for comparison, untreated group (control), ⁇ -MSH alone treatment
  • group 100 ⁇ g/mL arbutin, as shown in FIG. 8
  • Musa and pizzadu showed 98%, 77%, and 50% tyrosinase inhibitory activity, respectively, in that order.
  • Daeseok it did not show significant inhibitory activity.
  • red rosen was superior, which means that the tyrosinase inhibitory activity of red rosen itself, not the activity caused by cytotoxicity, may be relatively superior.
  • B16-F10 cells were treated with the plum seed extracts of each variety and ⁇ -MSH, and for comparison, the untreated group (control), ⁇ -MSH alone treatment group, 100 ⁇ g / mL arbutin treatment group and the experiment was conducted to investigate the melanin content, as shown in FIG.
  • arbutin a positive control, showed 46% inhibitory activity, and there was no significant difference compared with red rosen and pizza bean, and the effects on melanin synthesis and tyrosinase activity all showed similar trends in the four extracts.

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Abstract

The present invention relates to a whitening composition comprising a plum seed extract as an active ingredient, and more specifically, provides a skin-whitening cosmetic composition, a health food composition, and a composition for preventing or treating skin conditions caused by overproduction of melanin, each of which comprises a plum seed extract as an active ingredient. The plum seed extract according to the present invention has more stable and superior tyrosinase inhibitory activity compared to the ascorbic acid conventionally used as a whitening material, and thus can be used as a functional cosmetic material for skin whitening and can effectively prevent or treat abnormal skin pigmentation disorders such as liver spots, freckles, and sunspots.

Description

자두 씨 추출물을 유효성분으로 함유하는 미백용 조성물Whitening composition containing plum seed extract as an active ingredient
본 발명은 자두 씨 추출물을 유효성분으로 함유하는 미백용 조성물에 관한 것이다.The present invention relates to a composition for whitening containing a plum seed extract as an active ingredient.
멜라닌(melanin)은 일정량 이상의 자외선을 흡수하여 유해한 자외선이 인체 내로 침투하는 것을 차단하여 인체를 보호하는 역할을 하나, 이에 의해 멜라닌 생성이 증가되어 피부의 색소 침착 문제가 발생하게 된다. Melanin (melanin) acts to protect the human body by absorbing more than a certain amount of ultraviolet rays and blocking harmful ultraviolet rays from penetrating into the human body.
비정상적인 피부 색소 침착 문제를 야기하는 주요 원인인 멜라닌의 합성 과정을 살펴보면, 세포 내의 티로신(tyrosine)을 기질로 하여 티로시나제(tyrosinase), TRP-1(tyrosinase related protein-1), TRP-2 등의 효소에 의해 도파(3,4-dihydroxyphenylalanine, DOPA)를 거쳐 도파퀴논(dopaquinone)을 생성시키며, 도파퀴논으로부터 자발적인 반응과 효소 반응을 거쳐 공중합체 흑색 색소인 멜라닌이 생성된다.Looking at the synthesis process of melanin, which is the main cause of abnormal skin pigmentation, enzymes such as tyrosinase, TRP-1 (tyrosinase related protein-1), TRP-2 using tyrosine as a substrate in cells Dopaquinone is produced through dopa (3,4-dihydroxyphenylalanine, DOPA), and melanin, the black pigment of the copolymer, is produced through spontaneous reaction and enzymatic reaction from dopaquinone.
비정상적인 색소 침착을 막기 위한 방법으로 멜라닌 합성 과정 중의 일부 반응을 저해함으로써 상기 멜라닌의 생성을 감소시키는 방법이 일반적으로 용이하게 행해지고 있으며, 이러한 용도로서 종래는 아스코르브산(ascorbic acid), 코지산(kojic acid), 알부틴(arbutin), 하이드로퀴논(hydroquinone) 등과 같은 각종 식물 추출물이 사용되는 경우가 많았다. As a method to prevent abnormal pigmentation, a method for reducing the production of melanin by inhibiting some reactions in the melanin synthesis process is generally easily performed, and for this purpose, conventionally ascorbic acid, kojic acid ), arbutin, and various plant extracts such as hydroquinone were often used.
하지만, 이들 중 코지산은 티로시나제 활성 부위에 존재하는 구리 이온을 킬레이트 시켜 효소 활동을 저해하는 작용을 하는 것으로, 성능이 좋으나 화장품에 배합시 안전성에 문제가 있어 사용에 제한이 가해지고 있고, 하이드로퀴논은 피부에 대한 자극성이 높으며, 아스코르브산 역시 안전성 문제를 가지고 있는 실정이다.However, among them, kojic acid inhibits enzyme activity by chelating copper ions present at the tyrosinase active site. Although it has good performance, there is a problem with safety when formulated in cosmetics. It is highly irritating to the skin, and ascorbic acid also has a safety problem.
한편, 자두(Prunus salicina L.)는 우리나라 신라 시대 때부터 재배된 과실로 장미과 벚나무 속 자두 아속에 속한다. 우리나라에서 생산되는 자두는 대부분 동양계 자두로서, 70%가 경북에서 생산되는 국내의 주요 과실 중 하나이다. 자두는 연중 수확 시기가 이른 과일로 품종과 환경에 따라 조성에 차이가 있으나 유용 성분으로 천연 페놀 화합물을 다량 함유하고 있다. 최근 연구에 따르면 페놀 화합물이 활성산소 대신 산화하여 항산화, 항암, 항균 등에 효과가 있는 것으로 연구되고 있다. On the other hand, plum ( Prunus salicina L. ) is a fruit cultivated since the Silla period in Korea and belongs to the subgenus Prunus in the family Rosaceae. Most of the plums produced in Korea are oriental plums, and 70% of them are produced in Gyeongbuk, and it is one of the main fruits in Korea. Plums are fruits with an early harvest throughout the year, and although the composition differs depending on the variety and environment, it contains a large amount of natural phenolic compounds as useful ingredients. According to a recent study, phenolic compounds are oxidized instead of free radicals and are being studied to be effective in antioxidants, anticancer, antibacterial, etc.
자두는 주로 생식으로 섭취하거나 건자두, 잼, 넥타, 주스, 와인 등의 형태로 가공된다. 이러한 가공식품들은 주로 과육과 과피를 이용한 것으로 가공식품 제조 후 부산물인 씨가 발생하게 되며 대부분 폐기되고 있다. 최근 이러한 부산물을 활용하기 위한 여러 가지 연구가 진행되고 있으나, 피부 미백 또는 색소 질환 치료를 위한 자두 부산물에 대한 연구는 아직 미비한 실정이다.Plums are mainly consumed raw or processed into dried plums, jam, nectar, juice, and wine. These processed foods mainly use the flesh and peel, and after manufacturing the processed foods, seeds, which are by-products, are generated and most of them are discarded. Recently, various studies have been conducted to utilize these by-products, but studies on prune by-products for skin whitening or treatment of pigment diseases are still insufficient.
본 발명의 목적은 폐기되는 과실의 씨 추출물을 이용한 피부 미백용 조성물을 제공하는 데에 있다. An object of the present invention is to provide a composition for skin whitening using a seed extract of discarded fruit.
본 발명의 다른 목적은 폐기되는 과실의 씨 추출물을 이용한 멜라닌 과다 생성에 따른 피부 질환 예방 또는 치료용 조성물을 제공하는 데에 있다.Another object of the present invention is to provide a composition for preventing or treating skin diseases caused by excessive melanin production using a seed extract of discarded fruits.
상기의 목적을 달성하기 위하여, 본 발명은 자두 씨 추출물을 유효성분으로 함유하는 피부 미백용 화장료 조성물을 제공한다.In order to achieve the above object, the present invention provides a cosmetic composition for skin whitening containing a plum seed extract as an active ingredient.
본 발명은 자두 씨 추출물을 유효성분으로 함유하는 피부 미백용 건강식품 조성물을 제공한다.The present invention provides a health food composition for skin whitening containing a plum seed extract as an active ingredient.
본 발명은 자두 씨 추출물을 유효성분으로 함유하는 멜라닌 과다 생성에 따른 피부 질환 예방 또는 치료용 약학 조성물을 제공한다.The present invention provides a pharmaceutical composition for preventing or treating skin diseases caused by excessive melanin production, comprising a plum seed extract as an active ingredient.
또한, 본 발명은 자두 씨 추출물을 유효성분으로 함유하는 멜라닌 과다 생성에 따른 피부 질환 예방 또는 개선용 건강식품 조성물을 제공한다.In addition, the present invention provides a health food composition for preventing or improving skin diseases caused by excessive production of melanin containing the plum seed extract as an active ingredient.
본 발명에 따른 자두 씨 추출물, 특히 피자두 씨 추출물은 기존 미백 원료로 사용되는 아스코르브산과 비교하여 안정되고 우수한 티로시나제 저해 활성을 나타내므로, 피부 미백을 위한 화장료 조성물, 건강식품 조성물 등으로 활용할 수 있다.The plum seed extract, particularly the pizza bean seed extract, according to the present invention is stable and exhibits superior tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a cosmetic composition for skin whitening, a health food composition, and the like.
또한, 상기 자두 씨 추출물을 멜라닌 과다 생성에 따른 피부 질환 예방 또는 치료용 조성물로 이용함으로써, 과도한 멜라닌 생성으로 발생되는 기미, 주근깨, 검버섯 등의 비정상적 피부 색소 침착 질환들을 효과적으로 예방 또는 치료할 수 있다.In addition, by using the plum seed extract as a composition for preventing or treating skin diseases caused by excessive melanin production, abnormal skin pigmentation diseases such as spots, freckles, and age spots caused by excessive melanin production can be effectively prevented or treated.
본 발명에 따른 조성물은 과실의 부산물인 자두의 씨를 유효성분으로 이용함으로써, 폐기되는 자원을 효율적으로 활용하여 환경 보호 및 자원 절약에 도움을 줄 수 있다.The composition according to the present invention uses the seeds of plums, which are by-products of fruits, as an active ingredient, thereby efficiently utilizing wasted resources, thereby helping to protect the environment and save resources.
도 1은 본 발명의 일 실시예에 사용된 자두의 3가지 품종, 후무사(Prunus salicina Formosa), 피자두(Prunus salicina Lindl. cv. Soldam) 및 도담(Prunus salicina Dodam)의 단면 이미지이다.1 is a cross-sectional image of three varieties of plums used in an embodiment of the present invention, Prunus salicina Formosa , Prunus salicina Lindl. cv. Soldam , and Prunus salicina Dodam.
도 2는 본 발명의 일 실시예에 따른 자두 씨 추출물 제조방법을 나타낸 순서도이다.2 is a flowchart illustrating a method for preparing a plum seed extract according to an embodiment of the present invention.
도 3은 도 2에 따라 제조된 자두 씨 추출물의 자두 품종별 티로시나제 저해 활성을 확인한 그래프이다.3 is a graph confirming the tyrosinase inhibitory activity of the plum seed extract prepared according to FIG. 2 for each plum variety.
도 4는 도 2에 따라 제조된 피자두 씨 추출물의 농도에 따른 티로시나제 저해 활성을 확인한 그래프이다.4 is a graph confirming the tyrosinase inhibitory activity according to the concentration of the pizza bean seed extract prepared according to FIG. 2 .
도 5는 도 2에 따라 제조된 피자두 씨 추출물의 상온 보관 기간에 따른 티로시나제 저해 활성의 변화를 나타낸 그래프이다.5 is a graph showing the change in tyrosinase inhibitory activity according to the storage period at room temperature of the pizza bean seed extract prepared according to FIG. 2 .
도 6은 아스코르브산의 상온 보관 기간에 따른 티로시나제 저해 활성의 변화를 나타낸 그래프이다.6 is a graph showing the change in tyrosinase inhibitory activity according to the storage period of ascorbic acid at room temperature.
도 7은 대석, 후무사, 홍로센, 피자두 4종의 자두 씨 추출물의 세포 생존율을 확인한 결과 그래프이다 (각 데이터는 평균±SEM (n=3), *P<0.05, **P<0.01 and #P<0.001 vs. control).7 is a graph showing the cell viability of four types of plum seed extracts: Daeseok, Humusa, Hongrosen, and Pizzadu (each data is mean±SEM (n=3), *P<0.05, **P<0.01) and #P<0.001 vs. control).
도 8은 도 7의 4종 자두 씨 추출물의 세포 티로시나제 활성을 확인한 결과 그래프이다 (각 데이터는 평균±SEM (n=3), 위첨자는 Duncan’s multiple range test에 의해 P<0.05에서 유의적인 차이를 가지며, 이는 이하 도면에서도 동일하다).8 is a graph showing the results of confirming the cell tyrosinase activity of the four kinds of plum seed extracts of FIG. 7 (each data is mean±SEM (n=3), superscripts have a significant difference at P<0.05 by Duncan's multiple range test. , which is the same in the drawings below).
도 9는 도 7의 4종 자두 씨 추출물의 멜라닌 함량을 확인한 결과 그래프이다.9 is a graph showing the results of confirming the melanin content of the four kinds of plum seed extracts of FIG. 7 .
이하, 본 발명을 상세하게 설명하기로 한다.Hereinafter, the present invention will be described in detail.
본 발명자는 우리나라에서 주로 재배되는 자두인 후무사(Formosa), 피자두(Soldam), 도담(Dodam), 홍로센(Hongrosen) 및 대석(Oishiwase) 자두의 씨 추출물을 제조하여, 이들의 티로시나제 저해 활성 등을 확인함으로써, 본 발명을 완성하였다.The present inventors prepared seed extracts of plums mainly cultivated in Korea, such as hummusa ( Formosa ), pizza bean ( Soldam ), dodam ( Dodam ), Hongrosen ( Hongrosen ) and Daeseok ( Oishiwase ) plums, and their tyrosinase inhibitory activity By confirming etc., this invention was completed.
본 발명은 자두 씨 추출물을 유효성분으로 함유하는 피부 미백용 화장료 조성물을 제공한다.The present invention provides a cosmetic composition for skin whitening containing a plum seed extract as an active ingredient.
본 명세서에서, 상기 "자두 씨"는 자두(Plum)에서 과육 및 과피를 제거한 종자 부분으로, 피자두, 후무사, 도담, 대석, 홍로센, 홍자두, 대왕자두, 자봉자두, 게루시 및 추희로 이루어진 군에서 선택된 하나 이상의 자두의 씨일 수 있으나, 이에 제한되는 것은 아니다.In the present specification, the "plum seed" is a part of the seed from which the flesh and skin are removed from the plum (Plum). It may be one or more plum seeds selected from the group consisting of, but is not limited thereto.
바람직하게는 피자두(Prunus salicina Lindl. cv. Soldam), 후무사(Prunus salicina Formosa), 도담(Prunus salicina Dodam), 홍로센(Hongrosen) 및 대석(Oishiwase)으로 이루어진 군에서 선택된 하나 이상의 자두의 씨일 수 있고, 보다 바람직하게는 피자두(Prunus salicina Lindl. cv. Soldam) 또는 홍로센(Hongrosen)의 씨일 수 있다.Preferably, the seal of at least one plum selected from the group consisting of pizza beans (Prunus salicina Lindl. cv. Soldam ), Prunus salicina Formosa , Prunus salicina Dodam, Hongrosen and Oishiwase. It may be, and more preferably, the seeds of pizza bean (Prunus salicina Lindl. cv. Soldam ) or Hongrosen (Hongrosen).
상기 자두 씨는 자연에서 채취 또는 재배된 자두에서 수득한 것일 수 있고, 상업적으로 판매되는 것을 구입한 것일 수 있다.The plum seeds may be obtained from plums harvested or grown in nature, or purchased commercially.
본 명세서에서, "추출물"이란, 추출 방법, 추출 용매, 추출된 성분 또는 추출물의 형태를 불문하고 천연물의 성분을 뽑아냄으로써 얻어진 물질을 의미하는 것으로, 천연물의 성분을 뽑아내어 얻어진 물질을 추출 후 다른 방법으로 가공 또는 처리하여 얻어질 수 있는 물질을 모두 포함할 수 있다.As used herein, the term "extract" refers to a material obtained by extracting a component of a natural product regardless of an extraction method, an extraction solvent, an extracted component, or the form of an extract, and the material obtained by extracting the component of a natural product is extracted It may include any material obtainable by processing or processing by the method.
상기 자두 씨 추출물은 물, C1 내지 C4의 알코올 또는 이들의 혼합 용매로 추출된 것일 수 있고, 바람직하게는 60 내지 80중량% 에탄올 수용액 또는 프레타놀 수용액 추출물일 수 있으며, 보다 바람직하게는 70중량%의 식물성 에탄올인 프레타놀(prethanol) 추출물일 수 있다.The plum seed extract may be extracted with water, C1 to C4 alcohol, or a mixed solvent thereof, preferably 60 to 80% by weight of an aqueous ethanol solution or an aqueous pretanol solution, more preferably 70% by weight It may be a vegetable ethanol extract of pretanol (prethanol).
상기 자두 씨 추출물은 당해 기술 분야에서 통상적으로 사용하는 방법에 따라 추출될 수 있고, 예를 들어, 열수 추출, 초음파 추출법, 여과법, 환류 추출법 등이 있으며, 이들은 단독으로 수행되거나 2종 이상의 방법을 병용하여 수행될 수 있다.The plum seed extract may be extracted according to a method commonly used in the art, for example, hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc., which are performed alone or in combination of two or more methods can be performed.
본 명세서에서, "미백"은 멜라닌 색소 등의 과다 침착을 억제, 저해, 또는 완화시켜, 상기 색소 침착으로 야기되는 피부의 흑화를 방지하여 피부 트러블을 개선하는 작용을 의미한다. As used herein, "whitening" refers to an action of improving skin troubles by inhibiting, inhibiting, or alleviating excessive deposition of melanin pigment, etc., to prevent blackening of the skin caused by the pigmentation.
본 명세서에서, "화장료"란, 피부 색소 침착 등의 예방 또는 개선의 효과를 가지는 기능성 화장료를 포함할 수 있고, 상기 기능성 화장료는 일반 화장료와 달리 생리활성적인 효능, 효과가 강조된 전문적인 기능성을 갖는 것으로, 화장품법에 정의된 바에 따라 피부 미백 등 특정 효능과 효과가 강조된 화장료를 의미한다.In the present specification, the term "cosmetics" may include functional cosmetics having an effect of preventing or improving skin pigmentation, etc., and the functional cosmetics have specialized functionalities that emphasize physiologically active efficacy and effects, unlike general cosmetics. As defined by the Cosmetics Act, it refers to cosmetics emphasizing specific efficacy and effects such as skin whitening.
본 발명에 따른 피부 미백용 화장료 조성물에 있어서, 상기 자두 씨 추출물은 우수한 티로시나제(tyrosinase) 저해 활성을 가질 수 있다. 상술한 바와 같이, 티로시나제는 멜라닌 합성 과정에 이용되는 효소로, 이의 활성을 저해함으로써 멜라닌 생성을 억제하여 미백 효과를 부여할 수 있다.In the cosmetic composition for skin whitening according to the present invention, the plum seed extract may have excellent tyrosinase inhibitory activity. As described above, tyrosinase is an enzyme used in the melanin synthesis process, and by inhibiting its activity, it can inhibit melanin production to impart a whitening effect.
본 발명의 일 실험예에 따르면, 상기 자두 씨 추출물은 티로시나제 저해 활성을 가지는 것으로 나타났고, 특히, 피자두, 홍로센 씨 추출물에서 우수한 티로시나제 저해 활성이 나타남을 확인할 수 있었다.According to an experimental example of the present invention, the plum seed extract was found to have tyrosinase inhibitory activity, and in particular, it was confirmed that the pizza bean and red rosen seed extracts exhibited excellent tyrosinase inhibitory activity.
또한, 상기 자두 씨 추출물은 기존 미백 원료로 사용되는 아스코르브산에 비해 티로시나제 저해 활성에 대한 안정성이 현저히 우수한 것으로 나타나, 보다 안정적이고 효과적인 피부 미백용 화장료 조성물로 사용될 수 있다.In addition, the plum seed extract showed significantly superior stability to tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a more stable and effective cosmetic composition for skin whitening.
본 발명에 따른 피부 미백용 화장료 조성물에 있어서, 상기 자두 씨 추출물은 전체 화장료 100 중량부에 대하여 0.001 내지 50 중량부로 포함될 수 있으나, 이에 제한되는 것은 아니다.In the cosmetic composition for skin whitening according to the present invention, the plum seed extract may be included in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total cosmetic, but is not limited thereto.
본 발명에 따른 화장료 조성물은 식품 의약품 안전청에 등록된 화장품 원료 리스트 및 ICID(국제화장품 원료집)에 등재되어 있는 성분 중에서 1종 이상을 더 포함할 수 있다. 예를 들어, 상기 화장료 조성물은 유기 용매, 용해제, 농축제, 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 방향제, 계면활성제, 유화제, 충전제, 금속이온 봉쇄제, 방부제, 비타민, 차단제, 습윤화제, 염료 및 안료, 친수성 또는 친유성 활성제, 또는 화장품에 통상적으로 사용되는 임의의 다른 성분과 같은 화장품 분야에서 통상적으로 사용되는 보조제를 하나 이상 더 포함할 수 있다.The cosmetic composition according to the present invention may further include one or more of the ingredients listed in the list of cosmetic raw materials registered with the Food and Drug Administration and ICID (International Cosmetic Ingredients). For example, the cosmetic composition may include an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a fragrance, a surfactant, an emulsifier, a filler, a sequestering agent, a preservative, a vitamin, a blocking agent, It may further comprise one or more adjuvants commonly used in the cosmetic field, such as wetting agents, dyes and pigments, hydrophilic or lipophilic actives, or any other ingredients commonly used in cosmetics.
상기 비타민은 수용성 또는 유용성 비타민을 포함할 수 있고, 상기 수용성 비타민은 수용성 비타민으로서 화장품에 배합 가능한 것으로, 바람직하게는 비타민 B1, 비타민 B2, 비타민 B6, 피리독신, 염산피리독신, 비타민 B12, 판토텐산, 니코틴산, 니코틴산아미드, 엽산, 비타민C, 비타민 H 등을 들 수 있으며, 보다 바람직하게는 콜라겐 합성이 도움을 주는 비타민 C를 포함할 수 있다. 또한, 그들의 염 (티아민염산염, 아스코르빈산나트륨염 등)이나 유도체(아스코르빈산-2-인산나트륨염, 아스코르빈산-2-인산마그네슘염 등)도 포함될 수 있고, 상기 수용성 비타민은 미생물 변환법, 미생물의 배양물로부터의 정제법, 효소법 또는 화학 합성법 등의 통상의 방법에 의해 수득될 수 있다.The vitamin may include a water-soluble or oil-soluble vitamin, and the water-soluble vitamin is a water-soluble vitamin that can be formulated in cosmetics, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, and nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like, and more preferably, vitamin C that helps collagen synthesis. In addition, salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) or derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) may be included, and the water-soluble vitamin is a microorganism transformation method , can be obtained by a conventional method such as purification from a culture of microorganisms, enzymatic method or chemical synthesis method.
본 발명에 따른 화장료 조성물은 본 발명이 속하는 기술 분야에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있다. 예를 들어, 화장수, 유액, 로션, 크림, 페이스트, 젤, 용액, 현탁액, 오일, 왁스, 팩, 파우더, 파운데이션, 스프레이, 계면활성제-함유 클렌징 등으로 제형화 될 수 있으나, 이에 제한되는 것은 아니다. The cosmetic composition according to the present invention may be prepared in any formulation conventionally prepared in the technical field to which the present invention pertains. For example, it may be formulated as a lotion, emulsion, lotion, cream, paste, gel, solution, suspension, oil, wax, pack, powder, foundation, spray, surfactant-containing cleansing, etc., but is not limited thereto. .
더욱 상세하게는, 유연 화장수, 영양 화장수, 수렴 화장수, 영양 크림, 마사지 크림, 밀크 로션, 파우더, 에센스, 아이 크림, 선로션, 선크림, 메이크업 프라이머, 메이크업 베이스, 비비크림, 분말 파운데이션, 유탁액 파운데이션, 클렌징크림, 클렌징폼, 클렌징 워터, 비누, 팩, 스틱상 제품, 밤(Balm) 타입 제품, 스프레이 또는 파우더의 제형으로 제조될 수 있다.More specifically, flexible lotion, nourishing lotion, astringent lotion, nourishing cream, massage cream, milk lotion, powder, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, powder foundation, emulsion foundation , cleansing cream, cleansing foam, cleansing water, soap, pack, stick product, balm type product, spray or powder formulation.
본 발명에 따른 화장료 조성물이 크림 또는 젤 제형인 경우에는 담체 성분으로 동물성유, 식물성유, 왁스, 파라핀, 전분, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등을 더 포함할 수 있다.When the cosmetic composition according to the present invention is a cream or gel formulation, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be further included as a carrier component. can
상기 화장료 조성물이 용액 또는 유탁액 제형인 경우에는 용매, 용매화제 또는 유탁화제로, 물, 에탄올, 이소프로판올, 에틸카보네이트, 에틸아세테이트, 벤질알코올, 프로필렌글리콜, 글리세롤 지방족 에스테르, 폴리에틸렌글리콜 또는 소르비탄의 지방산 에스테르 등을 더 포함할 수 있다.When the cosmetic composition is a solution or emulsion formulation, a solvent, solvating agent or emulsifying agent, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid It may further include esters and the like.
상기 화장료 조성물이 현탁액 제형인 경우에는 담체 성분으로 물, 에탄올 또는 프로필렌 글리콜과 같은 액상 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등을 더 포함할 수 있다.When the cosmetic composition is a suspension formulation, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be further included.
상기 화장료 조성물이 파우더 또는 스프레이 제형인 경우에는 담체 성분으로 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더를 더 포함할 수 있고, 특히 스프레이 제형인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸에테르와 같은 추진체를 더 포함할 수 있다.When the cosmetic composition is in a powder or spray formulation, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be further included as a carrier component, and in particular, in the case of a spray formulation, additional chlorofluorohydrocarbon , may further include a propellant such as propane/butane or dimethyl ether.
상기 화장료 조성물이 계면활성제-함유 클렌징 제형인 경우에는 담체 성분으로 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성유, 리놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등을 더 포함할 수 있다.When the cosmetic composition is a surfactant-containing cleansing formulation, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components It may further include ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester.
본 발명에 따른 화장료 조성물은 단독 또는 중복으로 도포하여 사용하거나, 본 발명 이외의 다른 화장료 조성물과 중복 도포하여 사용할 수 있다. 또한, 본 발명에 따른 화장료 조성물은 통상적인 사용방법에 따라 사용될 수 있으며, 사용자의 피부 상태 또는 취향에 따라 그 사용횟수를 달리할 수 있다.The cosmetic composition according to the present invention may be used alone or by being applied in duplicate, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.
본 발명은 자두 씨 추출물을 유효성분으로 함유하는 피부 미백용 건강식품 조성물을 제공한다.The present invention provides a health food composition for skin whitening containing a plum seed extract as an active ingredient.
상기 자두 씨 추출물은 우수한 티로시나제 저해 활성을 가짐으로써, 멜라닌 생성을 억제하여 미백 효과를 부여할 수 있다. 본 발명의 일 실험예에 따르면, 상기 자두 씨 추출물은 티로시나제 저해 활성을 가지며, 특히, 피자두, 홍로센 씨 추출물에서 우수한 티로시나제 저해 활성이 나타남을 확인할 수 있었다. The plum seed extract has an excellent tyrosinase inhibitory activity, thereby inhibiting melanin production to impart a whitening effect. According to an experimental example of the present invention, it was confirmed that the plum seed extract had tyrosinase inhibitory activity, and in particular, excellent tyrosinase inhibitory activity was exhibited in the pizza bean and red rosen seed extracts.
또한, 상기 자두 씨 추출물은 기존 미백 원료로 사용되는 아스코르브산에 비해 티로시나제 저해 활성에 대한 안정성이 현저히 우수한 것으로 나타나, 보다 안정적이고 효과적인 피부 미백용 건강식품 조성물로 사용될 수 있다.In addition, the plum seed extract showed significantly superior stability to tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a more stable and effective health food composition for skin whitening.
이에 상응하는 특징들은 상술된 부분에서 대신할 수 있다.Corresponding features may be substituted for the above-mentioned parts.
본 발명에 따른 건강식품 조성물에 있어서, 상기 건강식품은 분말, 과립, 정제, 캡슐, 시럽 또는 음료 등으로 제조될 수 있고, 상기 건강식품이 취할 수 있는 형태에는 제한이 없으며, 통상적인 의미의 식품을 모두 포함할 수 있다. 예를 들어, 음료 및 각종 드링크, 과실 및 그의 가공식품(과일통조림, 잼 등), 어류, 육류 및 그 가공식품(햄, 베이컨 등), 빵류 및 면류, 쿠키 및 스낵류, 유제품(버터, 치즈 등) 등이 가능하며, 통상적인 의미에서의 기능성 식품을 모두 포함할 수 있다. 또한, 동물을 위한 사료로 이용되는 식품도 포함할 수 있다.In the health food composition according to the present invention, the health food may be prepared in powder, granule, tablet, capsule, syrup or beverage, etc. may include all of them. For example, beverages and various drinks, fruits and their processed foods (canned fruit, jam, etc.), fish, meat and their processed foods (ham, bacon, etc.), breads and noodles, cookies and snacks, dairy products (butter, cheese, etc.) ), etc. are possible, and may include all functional foods in a conventional sense. It may also include food used as feed for animals.
본 발명에 따른 건강식품 조성물은 당업계에서 통상적으로 사용되는 식품학적으로 허용 가능한 식품 첨가제 및 적절한 기타 보조 성분을 더 포함하여 제조될 수 있다. 예를 들어, 향미제, 천연 탄수화물, 감미제, 비타민, 전해질, 착색제, 펙트산, 알긴산, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산화제 등을 추가로 함유할 수 있다. The health food composition according to the present invention may be prepared by further including pharmaceutically acceptable food additives and other suitable auxiliary ingredients commonly used in the art. For example, flavoring agents, natural carbohydrates, sweetening agents, vitamins, electrolytes, coloring agents, pectic acid, alginic acid, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents, etc. can
특히, 상기 천연 탄수화물로는 포도당, 과당과 같은 모노사카라이드, 말토스, 수크로오스와 같은 디사카라이드, 및 덱스트린, 사이클로덱스트린과 같은 폴리사카라이드, 자일리톨, 소르비톨, 에리트리톨 등의 당알콜을 사용할 수 있으며, 감미제로서는 타우마틴, 스테비아 추출물과 같은 천연 감미제나 사카린, 아스파르탐과 같은 합성 감미제 등을 사용할 수 있다.In particular, as the natural carbohydrate, monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol can be used. , as the sweetener, natural sweeteners such as taumatine and stevia extract or synthetic sweeteners such as saccharin and aspartame may be used.
본 발명에 따른 건강식품에 함유된 자두 씨 추출물의 유효 용량은 피부 미백 목적에 따라 적절하게 조절될 수 있다. 상기 조성물은 천연물 및 식품을 원료로 하여 일반 약품의 장기 복용 시 발생할 수 있는 부작용 등이 없는 장점이 있고, 휴대성이 뛰어나, 피부 미백을 위한 보조제로 섭취될 수 있다.The effective dose of the plum seed extract contained in the health food according to the present invention may be appropriately adjusted according to the purpose of skin whitening. The composition has the advantage of not having side effects that may occur during long-term use of general drugs by using natural products and food as raw materials, and has excellent portability and can be taken as an auxiliary for skin whitening.
본 발명은 자두 씨 추출물을 유효성분으로 함유하는 멜라닌 과다 생성에 따른 피부 질환 예방 또는 치료용 약학 조성물을 제공한다.The present invention provides a pharmaceutical composition for preventing or treating skin diseases caused by excessive melanin production, comprising a plum seed extract as an active ingredient.
상기 자두 씨 추출물은 우수한 티로시나제 저해 활성을 가짐으로써, 멜라닌 생성을 억제할 수 있고, 이를 이용하여 멜라닌 과다 생성에 따른 피부 질환을 예방 또는 치료할 수 있다. Since the plum seed extract has excellent tyrosinase inhibitory activity, it is possible to inhibit melanin production, and by using it, it is possible to prevent or treat skin diseases caused by excessive melanin production.
또한, 상기 자두 씨 추출물은 기존 미백 원료로 사용되는 아스코르브산에 비해 티로시나제 저해 활성에 대한 안정성이 현저히 우수한 것으로 나타나, 보다 안정적이고 효과적인 상기 피부 질환의 치료 조성물로 사용될 수 있다.In addition, the plum seed extract showed significantly superior stability to tyrosinase inhibitory activity compared to ascorbic acid used as a conventional whitening raw material, and thus can be used as a more stable and effective composition for treating the skin disease.
이에 상응하는 특징들은 상술된 부분에서 대신할 수 있다.Corresponding features may be substituted for the above-mentioned parts.
본 발명에 따른 약학 조성물에 있어서, 상기 멜라닌 과다 생성에 따른 피부 질환은 기미, 주근깨, 잡티, 검버섯 및 오타모반으로 이루어진 군에서 선택되는 하나 이상의 색소 질환일 수 있으나, 이에 제한되는 것은 아니다. In the pharmaceutical composition according to the present invention, the skin disease caused by the excessive production of melanin may be one or more pigment diseases selected from the group consisting of melasma, freckles, blemishes, age spots and nevus Ota, but is not limited thereto.
본 명세서에서, "기미(melasma)"는 불규칙한 모양, 다양한 크기의 갈색 점이 노출부위, 특히 얼굴에 발생하는 색소성 질환으로, 태양 광선에 대한 노출 등에 의해 악화될 수 있다. 주로 좌우 대칭적으로 뺨과 이마, 눈 밑에 멜라닌 색소가 침착된다.As used herein, "melasma" is a pigmented disease in which brown spots of irregular shape and various sizes occur on exposed areas, especially on the face, and may be exacerbated by exposure to sunlight or the like. Melanin pigment is deposited mainly on the cheeks, forehead, and under the eyes symmetrically.
본 명세서에서, "주근깨(freckle)"는 햇빛에 노출된 부위의 피부에 주로 생기는 황갈색의 작은 색소성 반점으로, 원인은 정확하게 밝혀져 있지 않으나, 멜라노코르틴-1-수용체(melanocortin-1-receptor)의 유전자 변이와 관련이 있으며, 자외선에 의해 피부 멜라닌 세포가 자극을 받아 멜라닌 색소의 합성이 증가하여 발생할 수 있는 것으로 알려져 있다. As used herein, "freckle" is a small, yellowish-brown, pigmented spot that occurs mainly on the skin in areas exposed to sunlight, the cause of which is unknown, but melanocortin-1-receptor (melanocortin-1-receptor) It is related to the genetic mutation of , and it is known that it can occur because the synthesis of melanin pigment is increased by stimulation of skin melanocytes by ultraviolet rays.
본 명세서에서, "검버섯(liver spot, age spot)"은 노화 및 햇빛의 자외선 노출에 의해 형성된 피부 상의 얼룩으로, 일광흑색점(햇빛흑색점, 일광흑자, solar lentigo) 또는 간반이라고도 한다. As used herein, "liver spot, age spot" is a stain on the skin formed by aging and exposure to ultraviolet rays of sunlight, and is also referred to as sun melanoma (sunlight spot, solar lentigo) or ganban.
본 명세서에서, "오타모반(nevus of Ota)"은 눈 주위의 피부와 눈, 점막 등에 잘 발생하는 갈색 내지 푸른 색조를 띠는 반점을 말한다.As used herein, the term "nevus of Ota" refers to a spot having a brown or blue hue that occurs well on the skin, eyes, and mucous membranes around the eyes.
본 발명에 따른 약학 조성물은 약학적 분야의 통상적인 방법에 따라 제조될 수 있다. 상기 약학 조성물은 제형에 따라 약학적으로 허용가능한 적절한 담체와 배합될 수 있고, 필요에 따라, 부형제, 희석제, 분산제, 유화제, 완충제, 안정제, 결합제, 붕해제, 용제 등을 더 포함하여 제조될 수 있다. 상기 적절한 담체 등은 본 발명에 따른 자두 씨 추출물의 활성 또는 특성을 저해하지 않는 것으로, 투여 형태 및 제형에 따라 달리 선택될 수 있다.The pharmaceutical composition according to the present invention may be prepared according to a conventional method in the pharmaceutical field. The pharmaceutical composition may be combined with a suitable pharmaceutically acceptable carrier according to the formulation, and if necessary, excipients, diluents, dispersants, emulsifiers, buffers, stabilizers, binders, disintegrants, solvents, etc. may be prepared further comprising have. The appropriate carrier and the like do not inhibit the activity or properties of the plum seed extract according to the present invention, and may be selected differently depending on the dosage form and formulation.
본 발명에 따른 약학 조성물은 어떠한 제형으로도 적용될 수 있고, 보다 상세하게는 통상의 방법에 따라 경구형 제형, 외용제, 좌제 및 멸균 주사용액의 비경구형 제형으로 제형화하여 사용될 수 있다.The pharmaceutical composition according to the present invention can be applied in any dosage form, and more specifically, it can be used by formulating oral dosage forms, external preparations, suppositories, and parenteral dosage forms of sterile injection solutions according to conventional methods.
상기 경구형 제형 중 고형 제형은 정제, 환제, 산제, 과립제, 캡슐제 등의 형태로, 적어도 하나 이상의 부형제, 예를 들면, 전분, 칼슘카보네이트, 수크로스, 락토오스, 솔비톨, 만니톨, 셀룰로오스, 젤라틴 등을 섞어 조제할 수 있고, 단순한 부형제 이외에 마그네슘 스테아레이트, 탈크 같은 윤활제들도 포함될 수 있다. 또한, 캡술제형의 경우 상기 언급한 물질 외에도 지방유와 같은 액체 담체를 더 포함할 수 있다.The solid dosage form among the oral dosage forms is in the form of tablets, pills, powders, granules, capsules, etc., and at least one or more excipients, for example, starch, calcium carbonate, sucrose, lactose, sorbitol, mannitol, cellulose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc may be included. In addition, the capsule formulation may further include a liquid carrier such as fatty oil in addition to the above-mentioned substances.
상기 경구형 제형 중 액상 제형은 현탁제, 내용액제, 유제, 시럽제 등이 해당되는데 흔히 사용되는 단순 희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다.Among the oral dosage forms, liquid formulations include suspensions, solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients, for example, wetting agents, sweeteners, fragrances, preservatives, etc. may be included. have.
상기 비경구 제형은 멸균된 수용액, 비수성용제, 현탁제, 유제, 동결건조 제제, 좌제가 포함될 수 있다. 비수성용제, 현탁제로는 프로필렌글리콜, 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다. 좌제의 기제로는 위텝솔(witepsol), 마크로골, 트윈 61, 카카오지, 라우린지, 글리세로제라틴 등이 사용될 수 있다. 이에 제한되지 않고, 당해 기술 분야에 알려진 적합한 제제를 모두 사용 가능하다.The parenteral formulation may include a sterile aqueous solution, a non-aqueous solution, a suspension, an emulsion, a freeze-dried formulation, and a suppository. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, Tween 61, cacao butter, laurin fat, glycerogelatin, and the like can be used. Without being limited thereto, any suitable agent known in the art may be used.
또한, 본 발명에 따른 약학 조성물은 치료 효능의 증진을 위해 칼슘이나 비타민 D3 등을 더 첨가할 수 있다.In addition, the pharmaceutical composition according to the present invention may further add calcium, vitamin D3, or the like to enhance therapeutic efficacy.
본 발명에 따른 약학 조성물은 약학적으로 유효한 양으로 투여될 수 있다. The pharmaceutical composition according to the present invention may be administered in a pharmaceutically effective amount.
본 명세서에서, "약학적으로 유효한 양"이란, 의학적 치료에 적용 가능한 합리적인 수혜/위험 비율로 질환을 치료하기에 충분하며 부작용을 일으키지 않을 정도의 양을 의미한다.As used herein, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment and not to cause side effects.
상기 약학 조성물의 유효 용량 수준은 사용 목적, 환자의 연령, 성별, 체중 및 건강 상태, 질환의 종류, 중증도, 약물의 활성, 약물에 대한 민감도, 투여 방법, 투여 시간, 투여 경로 및 배출 비율, 치료기간, 배합 또는 동시 사용되는 약물을 포함한 요소 및 기타 의학 분야에 잘 알려진 요소에 따라 달리 결정될 수 있다. 예를 들어, 일정하지는 않지만 일반적으로 0.001 내지 100mg/kg으로, 바람직하게는 0.01 내지 10mg/kg을 일일 1회 내지 수회 투여될 수 있다. 상기 투여량은 어떠한 면으로든 본 발명의 범위를 한정하는 것은 아니다.The effective dose level of the pharmaceutical composition is determined by the purpose of use, the age, sex, weight and health status of the patient, the type of disease, severity, drug activity, sensitivity to drug, administration method, administration time, administration route and excretion rate, treatment It may be determined differently depending on factors including the duration, formulation or concurrent use of drugs and other factors well known in the medical field. For example, although not constant, generally 0.001 to 100 mg/kg, preferably 0.01 to 10 mg/kg, may be administered once to several times a day. The above dosage does not limit the scope of the present invention in any way.
본 발명에 따른 약학 조성물은 제제 형태에 따른 적당한 투여 경로로 투여될 수 있고, 목적 조직에 도달할 수 있는 한 경구 또는 비경구의 다양한 경로를 통하여 투여될 수 있다. 투여 방법은 특히 한정할 필요 없이, 예를 들면, 경구, 직장 또는 정맥, 근육, 피부 도포, 피하, 호흡기내 흡입, 자궁내 경막 또는 뇌혈관내(intracere-broventricular) 주사 등의 통상적인 방법으로 투여될 수 있다.The pharmaceutical composition according to the present invention may be administered by an appropriate administration route according to the form of the formulation, and may be administered through various routes, either oral or parenteral, as long as it can reach the target tissue. The administration method does not need to be particularly limited, and for example, oral, rectal or intravenous, muscle, skin application, subcutaneous, respiratory inhalation, intrauterine dural or intracerebroventricular injection, etc. are administered in a conventional manner. can be
본 발명에 따른 약학 조성물은 멜라닌 과다 생성에 따른 피부 질환의 예방 또는 치료를 위하여 단독으로 사용될 수 있고, 수술 또는 다른 약물 치료 등과 병용하여 사용될 수 있다.The pharmaceutical composition according to the present invention may be used alone for the prevention or treatment of skin diseases caused by excessive melanin production, or may be used in combination with surgery or other drug treatment.
또한, 본 발명은 자두 씨 추출물을 유효성분으로 함유하는 멜라닌 과다 생성에 따른 피부 질환 예방 또는 개선용 건강식품 조성물을 제공한다.In addition, the present invention provides a health food composition for preventing or improving skin diseases caused by excessive production of melanin containing the plum seed extract as an active ingredient.
이에 상응하는 특징들은 상술된 부분에서 대신할 수 있다.Corresponding features may be substituted for the above-mentioned parts.
또한, 본 발명은 자두 씨 추출물을 유효성분으로 함유하는 티로시나제의 활성 저해용 시약 조성물을 제공할 수 있다.In addition, the present invention may provide a reagent composition for inhibiting the activity of tyrosinase containing a plum seed extract as an active ingredient.
이하, 본 발명의 이해를 돕기 위하여 실시예를 들어 상세하게 설명하기로 한다. 다만 하기의 실시예는 본 발명의 내용을 예시하는 것일 뿐 본 발명의 범위가 하기 실시예에 한정되는 것은 아니다. 본 발명의 실시예는 당업계에서 평균적인 지식을 가진 자에게 본 발명을 보다 완전하게 설명하기 위해 제공되는 것이다.Hereinafter, to help the understanding of the present invention, examples will be described in detail. However, the following examples are merely illustrative of the contents of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.
<실시예 1> 후무사, 피자두, 도담 3종 품종별 자두 씨(종자) 추출물 제조.<Example 1> Preparation of plum seed (seed) extracts for each variety of Humusa, Pizza Doo, and Dodam.
본 실험에 사용된 자두는 도 1과 같이 후무사(Prunus salicina Formosa), 피자두(Prunus salicina Lindl. cv. Soldam) 및 도담(Prunus salicina Dodam) 3종으로, 각각 김천, 영천, 경산이 원산지로 온라인 및 과일가게에서 구입하여 과육으로부터 씨를 완전히 분리하여 씨만 실험에 사용하였다. As shown in FIG. 1, the plums used in this experiment are Prunus salicina Formosa , Prunus salicina Lindl. cv. Soldam , and Prunus salicina Dodam 3 types, respectively, from Gimcheon, Yeongcheon, and Gyeongsan. The seeds were completely isolated from the pulp, purchased online and at a fruit store, and only the seeds were used for the experiment.
씨를 분쇄기로 분쇄한 후, 분말 중량의 10배(v/w)의 식품 첨가용 에탄올인 70% 프레타놀 A(prethanol A, Duksan, Ansan, Gyeonggi-do, Korea)를 첨가하여 25℃, 50rpm에서 18시간씩 2회 반복 추출하였다. 추출물은 여과지(Whatman No.3, England)로 여과하여 여액은 45℃ 수욕 상에서 회전식 감압 농축기로 농축한 후 동결 건조하여 -20℃에 보관하며 실험에 사용하였다. 자세한 제조과정은 도 2와 같다.After pulverizing the seeds with a grinder, 70% pretanol A (prethanol A, Duksan, Ansan, Gyeonggi-do, Korea), which is ethanol for food addition at 10 times (v/w) of the weight of the powder, was added at 25℃, 50rpm. Extraction was repeated twice for 18 hours each. The extract was filtered through filter paper (Whatman No.3, England), and the filtrate was concentrated with a rotary vacuum concentrator on a 45°C water bath, then freeze-dried, stored at -20°C, and used in the experiment. A detailed manufacturing process is shown in FIG. 2 .
<실험예 1> 자두 씨 추출물의 티로시나제(tyrosinase) 저해 활성 확인<Experimental Example 1> Confirmation of tyrosinase inhibitory activity of plum seed extract
티로시나제(tyrosinase) 저해 활성 측정은 0.1M 인산 칼륨 완충액(potassium phosphate buffer, pH 7.0) 80μL, 0.01 M L-도파(L-Dopa) 40μL와 시료 용액 40μL의 혼합액에 버섯류 티로시나제(mushroom tyrosinase) 125U/mL를 첨가하여 37℃ 에서 3분간 반응시켜 반응액 중에 생성되는 도파크롬(dopachrome)을 475nm에서 측정하였다. Tyrosinase inhibitory activity was measured in a mixture of 80 μL of 0.1 M potassium phosphate buffer (pH 7.0), 40 μL of 0.01 M L-Dopa and 40 μL of sample solution, and 125 U/mL of mushroom tyrosinase. was added and reacted at 37° C. for 3 minutes, and the dopachrome generated in the reaction solution was measured at 475 nm.
그 결과, 실시예 1에 따라 제조한 품종별 자두 씨 추출물은 도 3과 같이, 피자두 씨 추출물이 가장 높은 티로시나제 저해 활성을 나타내는 것으로 나타났다.As a result, as shown in FIG. 3, in the plum seed extracts prepared according to Example 1, the pizza bean seed extract showed the highest tyrosinase inhibitory activity.
이에, 티로시나제 저해 활성이 가장 우수한 피자두 씨 추출물의 농도별 저해 활성을 비교한 결과, 도 4와 같이, 0.25~1.5 mg/mL 농도 구간에서 농도에 의존적으로 높은 저해활성을 보였다.Accordingly, as a result of comparing the inhibitory activity by concentration of the pizza bean seed extract having the best tyrosinase inhibitory activity, as shown in FIG. 4 , the concentration-dependent high inhibitory activity was shown in the 0.25-1.5 mg/mL concentration range.
<실험예 2> 상온(RT)에서 보관 기간에 따른 티로사나제 저해 활성 변화 비교<Experimental Example 2> Comparison of changes in tyrosanases inhibitory activity according to storage period at room temperature (RT)
피자두 씨 추출물 및 아스코르브산(ascorbic acid, Junsei Chemical Co., Ltd. Tokyo, Japan)을 농도별로 제조한 후, 상온에 방치하며, 방치 후 4일, 8일, 12일, 20일째 각각의 티로시나제 저해 활성을 측정하였다.After preparing pizza bean seed extract and ascorbic acid (ascorbic acid, Junsei Chemical Co., Ltd. Tokyo, Japan) by concentration, left at room temperature, 4 days, 8 days, 12 days, and 20 days after leaving each tyrosinase Inhibitory activity was measured.
그 결과, 도 5와 같이, 피자두 씨 추출물의 경우 8일째까지 티로시나제 저해 활성의 유의적인 변화가 없었고, 12일째 미미한 티로시나제 저해 활성 변화가 나타났으며, 20일째는 제조 첫날 시료의 티로시나제 저해 활성 대비 약 40~50%정도 상기 저해 활성이 감소된 것을 확인할 수 있었다. As a result, as shown in FIG. 5 , in the case of the Pizza bean seed extract, there was no significant change in tyrosinase inhibitory activity until the 8th day, and a slight change in tyrosinase inhibitory activity on the 12th day was observed. It was confirmed that the inhibitory activity was reduced by about 40-50%.
이에 반해, 대표적인 미백 원료로 알려진 아스코르브산은 도 6과 같이, 8일째 25μg/mL 농도에서 첫날 대비 티로시나제 저해 활성이 78%정도 감소하였으며, 20일째는 모든 농도에서 90% 가까이 저해 활성이 감소된 것을 확인할 수 있었다. On the other hand, ascorbic acid, which is known as a representative whitening raw material, decreased about 78% in tyrosinase inhibitory activity compared to the first day at a concentration of 25 μg/mL on the 8th day, as shown in FIG. 6, and it was confirmed that the inhibitory activity was reduced by nearly 90% at all concentrations on the 20th could
결과적으로 티로시나제 저해 활성에 대한 안정성의 경우, 피자두 씨 추출물이 아스코르브산보다 월등히 우수한 것으로 확인되었다.As a result, in terms of stability against tyrosinase inhibitory activity, it was confirmed that the pizza bean seed extract was significantly superior to ascorbic acid.
<실시예 2> 대석, 홍로센 품종의 자두 씨 추출물 제조<Example 2> Preparation of Plum Seed Extract of Daeseok, Red Rosen Varieties
상기 실시예 1에 따른 방법으로 대석(Oishiwase), 홍로센(Hongrosen) 품종의 자두 씨 추출물을 추가로 제조하였다.A plum seed extract of Daeseok (Oishiwase ) and Hongrosen (Hongrosen) varieties was additionally prepared by the method according to Example 1.
<실험예 3> 대석, 후무사, 홍로센, 피자두 4종의 자두 씨 추출물의 생리활성 분석<Experimental Example 3> Analysis of physiological activity of plum seed extracts of 4 kinds of Daeseok, Humusa, Hongrosen, and Pizza bean
1. 실험방법1. Experimental method
1) 세포배양1) Cell culture
실험에 사용된 B16-F10 세포는 한국세포주은행에서 분양받아 사용하였다. 세포는 10% 소태아혈청(fetal bovine serum)과 100unit/mL 페니실린(penicillin) 및 100mg/mL 스트렙토마이신(streptomycin)을 첨가한 Dulbecco’s modified Eagle’s medium (DMEM) 배지를 이용하여 5% CO2와 37℃ 조건에서 계대 배양하며 사용하였다.B16-F10 cells used in the experiment were purchased from the Korea Cell Line Bank and used. Cells were prepared using Dulbecco's modified Eagle's medium (DMEM) medium supplemented with 10% fetal bovine serum, 100unit/mL penicillin, and 100mg/mL streptomycin at 5% CO 2 and 37°C. It was used while subcultured under the conditions.
2) 세포 생존율 측정2) Cell viability measurement
B16-F10 세포를 48-well에 0.5×104 cells/well이 되도록 분주하여 24시간 동안 배양하였다. 배양액을 제거하고 4종의 품종별 자두 씨 추출물을 농도별로 처리하여 5% CO2, 37℃조건의 인큐베이터에서 68시간 동안 배양한 후, 2.5mg/mL 농도로 제조한 MTT 용액을 20μL 처리하여 4시간 배양하였다. 그 후 배양액을 제거하고 dimethyl sulfoxide(디메틸 설폭사이드, DMSO)를 첨가하여 살아있는 세포에서 생성된 MTT-formazan 결정체를 용해시켜 550nm에서 흡광도를 측정하였다. B16-F10 cells were aliquoted into 48-wells so as to become 0.5×10 4 cells/well and cultured for 24 hours. After removing the culture medium and treating the four varieties of plum seed extracts by concentration, incubated for 68 hours in an incubator at 5% CO 2 , 37° C., 20 μL of MTT solution prepared at a concentration of 2.5 mg/mL was treated with 4 time incubation. After that, the culture medium was removed and dimethyl sulfoxide (dimethyl sulfoxide, DMSO) was added to dissolve the MTT-formazan crystals generated in living cells, and absorbance was measured at 550 nm.
3) 세포 티로시나제(cellular tyrosinase) 저해 활성 측정3) Measurement of cellular tyrosinase inhibitory activity
Cellular tyrosinase 저해 활성 측정을 위해서는 우선 B16-F10 세포를 48-well에 0.5×104 cells/well이 되게 분주하고 24시간 동안 배양한 후 품종별 자두 씨 추출물을 처리하였다. 추출물을 처리하고 1시간 후 1μM α-멜라닌세포 자극 호르몬(α-melanocyte stimulating hormone, α-MSH)을 처리하고 72시간 배양한 후 배양액을 제거하고 PBS(phosphate buffered saline)로 세척하였다. 100μL의 reporter lysis buffer를 첨가하여 세포를 용해시켜 1,000rpm, 4℃에서 5분간 원심분리하고, 상등액을 수집한 후 상등액 40μL에 10mM L-DOPA 100μL를 첨가하여 37℃에서 2시간 반응시킨 후 475nm에서 흡광도를 측정하였다.In order to measure the cellular tyrosinase inhibitory activity, B16-F10 cells were first aliquoted into 48-wells at a volume of 0.5×10 4 cells/well, cultured for 24 hours, and then treated with plum seed extracts for each variety. After 1 hour of treatment with the extract, 1 μM α-melanocyte stimulating hormone (α-MSH) was treated and incubated for 72 hours, the culture medium was removed and washed with PBS (phosphate buffered saline). Add 100 μL of reporter lysis buffer to lyse the cells, centrifuge at 1,000 rpm, 4° C. for 5 minutes, collect the supernatant, add 100 μL of 10 mM L-DOPA to 40 μL of the supernatant, react at 37° C. for 2 hours, and then at 475 nm. Absorbance was measured.
4) 멜라닌(Melanin) 함량 측정4) Melanin content measurement
Melanin 함량 측정을 위해서는 우선 B16-F10 세포를 6-well에 0.5×104 cells/well이 되게 분주하고 24시간 동안 배양한 후 품종별 자두 씨 추출물을 처리하였다. 1시간 후 1μM α-MSH를 처리하고 72시간 배양한 후 배양액을 제거하고 PBS로 세척하였다. 그 후 PBS 100μL를 분주하여 5,000 rpm, 4℃에서 5분간 원심분리하여 상등액을 버리고 펠렛(pellet)에 10% DMSO가 함유된 1N NaOH 용액을 400μL씩 첨가하여 80℃에서 1시간 동안 용해한 후 405nm에서 흡광도를 측정하였다.To measure the melanin content, first, B16-F10 cells were aliquoted into 6-wells so that 0.5×10 4 cells/well were cultured for 24 hours, and then the plum seed extracts for each variety were treated. After 1 hour, 1 μM α-MSH was treated, cultured for 72 hours, the culture medium was removed and washed with PBS. Then, 100 μL of PBS was dispensed, centrifuged at 5,000 rpm, 4° C. for 5 minutes, the supernatant was discarded, and 400 μL of 1N NaOH solution containing 10% DMSO was added to the pellet each, dissolved at 80° C. for 1 hour, and then dissolved at 405 nm. Absorbance was measured.
5) 통계분석5) Statistical analysis
실험결과에 대한 통계분석은 SPSS Statistics 25 (Statistical Package for the Social Science, Version 25.0 SPSS Inc., Chicago, IL, USA)를 사용하였다. 모든 데이터는 3회 이상 반복한 결과를 평균과 표준오차(mean±SEM)로 나타내었다. 그룹 간의 유의성 차이 검증에는 일원배치 분산분석(one-way ANOVA)을 사용하였고, Duncan's multiple range test를 이용한 사후 검증을 시행하였다. 또한, 95% (P < 0.05)의 유의 수준에서 각 구간의 유의적 차이를 검증하였다.For statistical analysis of the experimental results, SPSS Statistics 25 (Statistical Package for the Social Science, Version 25.0 SPSS Inc., Chicago, IL, USA) was used. All data were repeated three or more times and expressed as the mean and standard error (mean±SEM). One-way ANOVA was used to verify the difference in significance between groups, and a post-hoc test using Duncan's multiple range test was performed. In addition, significant differences in each interval were verified at a significance level of 95% (P < 0.05).
2. 실험결과2. Experimental results
1) 자두 씨 추출물의 세포 생존율 확인1) Confirmation of cell viability of plum seed extract
대석, 후무사, 홍로센, 피자두 4종 품종별 자두 씨 추출물이 B16-F10 세포에 독성을 나타내는지의 여부를 알아보기 위해 3-(4,5-디메틸치아졸일-2)-2,5-디페닐 테트라졸리움 브로미드[3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide, MTT] 분석(MTT assay)를 실시하였다. MTT assay는 미토콘드리아의 탈수소효소와 노란색의 MTT tetrazolium이 반응하여 형성된 자주색의 MTT formazan의 생성량을 측정하는 검사법이며, 흡광도는 550nm 근처 파장에서 최대가 된다. 이는 많은 시료를 간단하게 판독할 수 있어 세포독성 및 세포증식 검색법으로 널리 사용되고 있는 방법 중 하나이다.3-(4,5-dimethylthiazolyl-2)-2,5- Diphenyl tetrazolium bromide [3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide, MTT] assay (MTT assay) was performed. MTT assay is a test method to measure the amount of purple MTT formazan produced by the reaction of mitochondrial dehydrogenase with yellow MTT tetrazolium, and the absorbance is maximized at a wavelength near 550 nm. This is one of the methods widely used as a cytotoxicity and cell proliferation detection method because many samples can be read simply.
품종별 자두 씨 추출물의 B16-F10의 세포 생존율을 측정하기 위해 200-500μg/mL의 농도로 각 시료를 처리한 후, MTT assay를 실시한 결과, 도 7에 나타난 바와 같이, 시료 처리 결과 모든 품종의 자두 씨 추출물 400μg/mL에서 대석 73%, 후무사 79%, 홍로센 70%, 피자두 70%의 생존율을 나타내었다. 따라서 추후 실험은 세포독성이 30% 이하인 400μg/mL 농도에서 시행하였다.To measure the cell viability of B16-F10 in the plum seed extract for each variety, each sample was treated at a concentration of 200-500 μg/mL, and then MTT assay was performed. At 400 μg/mL of plum seed extract, 73% of Daeseok, 79% of Humusa, 70% of red rosen, and 70% of pizza bean showed survival rates. Therefore, subsequent experiments were carried out at a concentration of 400 μg/mL with a cytotoxicity of 30% or less.
2) 세포 티로시나제 저해 활성 및 멜라닌 함량 확인2) Confirmation of cell tyrosinase inhibitory activity and melanin content
α-MSH는 멜라닌 생성 유도물질로, 뇌하수체 및 피부를 포함한 여러 가지 말초조직에서 분비되며, 멜라닌 색소 생성, 표피세포의 성장과 증식, 국소조직의 면역조절 등 다양한 생리적 기능에 관여한다. α-MSH is a melanogenesis inducer, secreted from various peripheral tissues including the pituitary gland and skin, and is involved in various physiological functions such as melanin production, epidermal cell growth and proliferation, and local tissue immune regulation.
품종별 자두 씨의 티로시나제 저해 활성을 측정하기 위해 B16-F10 세포에 400μg/mL의 자두 씨 추출물과 1μM의 α-MSH을 함께 처리하고, 비교를 위하여 무처리군(control), α-MSH 단독 처리군, 100μg/mL 알부틴(arbutin)과 함께 실험한 결과, 도 8에 나타난 바와 같이, α-MSH 단독 처리군의 경우에는 티로시나제 활성이 무처리군에 비해 확연히 증가한 것을 확인할 수 있었으며, 홍로센, 후무사, 피자두 순으로 각각 98%, 77%, 50%의 티로시나제 저해 활성을 보였다. 반면, 대석의 경우 유의적인 저해활성을 나타내지 않았다. To measure the tyrosinase inhibitory activity of plum seeds by variety, B16-F10 cells were treated with 400 μg/mL of plum seed extract and 1 μM of α-MSH, and for comparison, untreated group (control), α-MSH alone treatment As a result of testing with the group, 100 μg/mL arbutin, as shown in FIG. 8, in the case of the α-MSH alone treatment group, it was confirmed that the tyrosinase activity was significantly increased compared to the untreated group, and after Musa and pizzadu showed 98%, 77%, and 50% tyrosinase inhibitory activity, respectively, in that order. On the other hand, in the case of Daeseok, it did not show significant inhibitory activity.
한편, 후무사, 홍로센, 피자두의 씨 추출물의 경우 세포 독성에 의한 효과도 일부 나타날 수 있을 것으로 예상되나, 홍로센과 피자두의 경우 400μg/mL의 농도에서 세포 독성은 비슷하지만, 티로시나제 저해 활성 결과는 홍로센이 더 우수한 것으로 나타났으며 이는 세포 독성에 의한 활성이 아닌 홍로센 자체의 티로시나제 저해 활성이 상대적으로 우수할 수 있음을 의미한다.On the other hand, in the case of the seed extracts of Humusa, Red Rosen, and Pizza bean, it is expected that some effects due to cytotoxicity may appear. The result showed that red rosen was superior, which means that the tyrosinase inhibitory activity of red rosen itself, not the activity caused by cytotoxicity, may be relatively superior.
α-MSH에 의해 유도된 멜라닌 합성 조건에서 품종별 자두 씨 추출물의 멜라닌 합성 억제효과를 확인하기 위하여 B16-F10 세포에 품종별 자두 씨 추출물과 α-MSH를 함께 처리하고, 비교를 위하여 무처리군(control), α-MSH 단독 처리군, 100μg/mL 알부틴 처리군과 함께 실험을 진행하여 멜라닌 함량을 조사한 결과, 도 9에 나타난 바와 같이, α-MSH 단독 처리군의 경우에는 멜라닌 함량이 무처리군에 비해 확연히 증가하였으나 추출물을 처리한 군들에서는 홍로센, 피자두, 후무사, 대석(92%, 87%, 57%, 42%) 순으로 높은 멜라닌 합성 저해활성이 관찰되었다. In order to check the melanin synthesis inhibitory effect of the plum seed extracts by variety under the α-MSH-induced melanin synthesis conditions, B16-F10 cells were treated with the plum seed extracts of each variety and α-MSH, and for comparison, the untreated group (control), α-MSH alone treatment group, 100μg / mL arbutin treatment group and the experiment was conducted to investigate the melanin content, as shown in FIG. 9, in the case of the α-MSH alone treatment group, the melanin content was not Although it was significantly increased compared to the group, high melanin synthesis inhibitory activity was observed in the extract-treated groups in the order of red rosen, pizzadoo, humusa, and daeseok (92%, 87%, 57%, 42%).
한편 양성대조군인 알부틴은 46%의 저해 활성을 나타내 홍로센 및 피자두와 비교해 유의적인 차이가 없었으며, 멜라닌 합성 및 티로시나제 활성에 미치는 영향 모두 네 가지 추출물에서 유사한 경향으로 나타났다.On the other hand, arbutin, a positive control, showed 46% inhibitory activity, and there was no significant difference compared with red rosen and pizza bean, and the effects on melanin synthesis and tyrosinase activity all showed similar trends in the four extracts.
이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다. 즉, 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다.As described above in detail a specific part of the content of the present invention, for those of ordinary skill in the art, it is clear that this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby. do. That is, the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (10)

  1. 자두 씨 추출물을 유효성분으로 함유하는 피부 미백용 화장료 조성물.A cosmetic composition for skin whitening containing plum seed extract as an active ingredient.
  2. 제 1 항에 있어서,The method of claim 1,
    상기 자두 씨는,The plum seed,
    피자두, 후무사, 도담, 대석, 홍로센, 홍자두, 대왕자두, 자봉자두, 게루시 및 추희로 이루어진 군에서 선택된 하나 이상의 자두의 씨인 것을 특징으로 하는 피부 미백용 화장료 조성물.A cosmetic composition for skin whitening, characterized in that it is the seed of one or more plums selected from the group consisting of pizza bean, hummusa, dodam, daeseok, red rosen, hong plum, giant plum, jabon plum, gerushi, and chuhee.
  3. 제 1 항에 있어서,The method of claim 1,
    상기 자두 씨 추출물은,The plum seed extract is
    물, C1 내지 C4의 알코올 또는 이들의 혼합 용매로 추출된 것을 특징으로 하는 피부 미백용 화장료 조성물.A cosmetic composition for skin whitening, characterized in that it is extracted with water, C1 to C4 alcohol, or a mixed solvent thereof.
  4. 제 1 항에 있어서, The method of claim 1,
    상기 자두 씨 추출물은,The plum seed extract is
    티로시나제(tyrosinase) 저해 활성을 가지는 것을 특징으로 하는 피부 미백용 화장료 조성물.A cosmetic composition for skin whitening, characterized in that it has tyrosinase inhibitory activity.
  5. 제 1 항에 있어서,The method of claim 1,
    상기 자두 씨 추출물은,The plum seed extract is
    전체 화장료 100 중량부에 대하여 0.001 내지 50 중량부로 포함되는 것을 특징으로 하는 피부 미백용 화장료 조성물.A cosmetic composition for skin whitening, characterized in that it is included in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total cosmetic.
  6. 제 1 항에 있어서,The method of claim 1,
    상기 조성물은,The composition is
    유연화장수, 영양화장수, 영양크림, 에센스 또는 팩의 제형을 갖는 것을 특징으로 하는 피부 미백용 화장료 조성물.A cosmetic composition for skin whitening, characterized in that it has the formulation of a softening lotion, nutrient lotion, nutrient cream, essence or pack.
  7. 자두 씨 추출물을 유효성분으로 함유하는 피부 미백용 건강식품 조성물.A health food composition for skin whitening containing plum seed extract as an active ingredient.
  8. 자두 씨 추출물을 유효성분으로 함유하는 멜라닌 과다 생성에 따른 피부 질환 예방 또는 치료용 약학 조성물.A pharmaceutical composition for preventing or treating skin diseases caused by excessive melanin production, comprising plum seed extract as an active ingredient.
  9. 제 8 항에 있어서,9. The method of claim 8,
    상기 피부 질환은,The skin disease is
    기미, 주근깨, 잡티, 검버섯 및 오타모반으로 이루어진 군에서 선택되는 하나 이상의 색소 질환인 것을 특징으로 하는 피부 질환 예방 또는 치료용 약학 조성물.A pharmaceutical composition for preventing or treating skin diseases, characterized in that it is one or more pigment diseases selected from the group consisting of spots, freckles, blemishes, age spots and nevus Ota.
  10. 자두 씨 추출물을 유효성분으로 함유하는 멜라닌 과다 생성에 따른 피부 질환 예방 또는 개선용 건강식품 조성물.A health food composition for preventing or improving skin diseases caused by excessive melanin production, comprising plum seed extract as an active ingredient.
PCT/KR2021/001326 2020-02-19 2021-02-02 Whitening composition comprising plum seed extract as active ingredient WO2021167269A1 (en)

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KR20180093560A (en) * 2017-02-14 2018-08-22 (주)튜링겐코리아 A composition for skin whitening, anti-oxidating, astringent or anti-wrinkle comprising extracts of unripe plum

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