WO2021133368A1 - Procédé d'élevage d'acariens prédateurs neoseiulus reductus - Google Patents

Procédé d'élevage d'acariens prédateurs neoseiulus reductus Download PDF

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Publication number
WO2021133368A1
WO2021133368A1 PCT/UA2020/000105 UA2020000105W WO2021133368A1 WO 2021133368 A1 WO2021133368 A1 WO 2021133368A1 UA 2020000105 W UA2020000105 W UA 2020000105W WO 2021133368 A1 WO2021133368 A1 WO 2021133368A1
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Prior art keywords
predatory
substrate
population
mites
mite
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PCT/UA2020/000105
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English (en)
Russian (ru)
Inventor
Ярослав Олегович ИВАНОВ
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Ярослав Олегович ИВАНОВ
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Publication of WO2021133368A1 publication Critical patent/WO2021133368A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates

Definitions

  • the invention relates to agriculture, in particular to biological protection of plants from pests.
  • Tetranychidae damage leaves which leads to a general weakening of the culture, deformation of vegetative organs and fruits, thereby reducing the value of the gross yield and its quality. (Ivanova G.P. et al, 2002).
  • acariphages will allow to produce clean products and save the environment from pollution.
  • a known method of breeding predatory mites (patent RU2351126, Published: 10.04.2009 bull. N ° 10).
  • the method includes growing a flour mite on sterilized and moistened bran and infecting it with a mite Amblyseius. To do this, use bran with a particle size of 1.0-2.0 mm, moisten them by spraying with sterile water at the rate of 750 ml of water per 10 liters of substrate. Then, the bran placed in cuvettes with a layer of 5-7 cm is infected with a flour mite.
  • Infection with the Amblyseius mite is carried out after 14-15 days at a flour mite density of 9.5-10 thousand individuals / cm 3 and at a ratio of 1: 50-1: 100, while the bran layer in the cuvettes is brought to 7-10 cm, and the collection the Amblyseius tick is carried out in 14-15 days.
  • the disadvantage of this method is the presence of laborious breeding stages, such as placement in glass containers, placement in plastic boxes, decontamination of these containers, as well as collection of predatory mites at the end of each cycle, with a rather small increase in Amblyseius cucumeris and a long period of its accumulation.
  • Neoseiulus californicus Wei-Lan Ma and Laing, J.E., 1973
  • Neoseiulus Reductus Kolodochka L.A., Lysaya E.A., 1976
  • Mass breeding includes production and ends with the collection of predatory mites, after which the most suitable representatives of phytoseiids are selected from the point of view of a specialist technologist, for breeding - launching a new production cycle.
  • the disadvantages of this method include the instability of the amount of produced acariphage (predatory mite), (depending on the season, from 1 m 3 are collected from 13 to 40 thousand individuals of the predator), a long period of its accumulation (35-55 days), as well as the method is energy and resource-intensive
  • the utility model is based on the task of increasing the productivity of the predatory mite production, as well as reducing the maturation of the biomaterial.
  • the problem is solved in a method for breeding predatory mites, in which the population of predators is combined with a population of alternative species of food mites, individuals of predators are given the opportunity to feed on individuals of the population of food ticks, according to the invention, the population of predators Neoseiulus reductus is used as a predatory tick, and ticks are used as a food tick from the Acariformes family, for example, Tyreophagu sentomophagus, wherein the method includes preparing a fodder substrate either on wheat bran or buckwheat or rice husks, or sawdust with a particle size of 2-4 mm, which are pre-disinfected by keeping at a temperature of 90 ° C for 5 hours are then moistened at the rate of 20-40 ml of water per 1000 ml of bran and placed in a closed container with ventilation, and the volume of the substrate is 50% of the volume of the container and placed on the substrate of the female predatory mite Neoseiul
  • Neoseiulus Reductus is carried out in a free-flowing carrier such as sieved wheat bran with a particle size of 2-4 mm, also buckwheat husks, rice husks, millet husks, sawdust, and mixtures thereof.
  • the substrate serves as a cultivation medium and a carrier for the transport and dispersal of predatory mites on plants to combat phytophagous invertebrates.
  • the proposed method for growing predatory mites has high productivity and is economically feasible.
  • the density of the Neoseiulus reductus population increases by 1.3-4 times compared to the density when set.
  • the productivity of the proposed technical solution is 5.5 - 6.5 million OS / m 3 of the production area.
  • the productivity of the proposed technical solution is 5.5 - 6.5 million OS / m2 of production area.
  • the biomaterial accumulation period is 7-14 days.
  • Neoseiulus reductus is grown using Tyreophagus entomophagus as a laboratory host, produced as follows: a feed substrate carrier of wheat bran is prepared, previously disinfected at a temperature of 90 ° C for 5 hours, moistened at the rate of 20-40 ml of water per 1000 ml of bran.
  • the fodder substrate is placed in a sealed container with ventilation, which is a plastic bucket from 1 to 10 liters with a tight-fitting lid.
  • the volume of the substrate in the container is from 1 to 5 liters.
  • Neoseiulus reductus females of the predatory mite Neoseiulus reductus are launched and the feeding mite Tyreophagus Entomophagus is introduced in a predator-prey ratio of 1: 5-50.
  • the population density of Neoseiulus reductus should start from 5 to 50 individuals / cm 3 . Further, this system is placed for building up in a room with a temperature (21-25) 0 ⁇ and air humidity (40-70)%. To prevent caking, it is necessary to stir the substrate daily and monitor the quality of the biomaterial. After 7-14 days, the number of predatory mites increases, while the number of forage mites decreases and the accumulation cycle stops, as the system depletes resources. Next, the population density of the predator is taken into account, the number of individuals per unit volume of the substrate (individuals / cm 3 ) is determined.
  • the population growth rate in the container is calculated by comparing the population density when entering the container and the population density at the end of the culture cycle.
  • This indicator can be from 1.3 to 4 (examples are tables 1.1, 1.3, 1.5), but should average 1.5-3.
  • the material is cultivated by mixing part of the substrate with a predator after cultivation with a new forage substrate, previously disinfected and moistened , at the rate of 20-40 ml of water / 1000 ml of substrate, add Tyreophagus entomophagus, in a ratio of 1: 5-1: 50 individuals / cm 3 , depending on the number of Neoseiulus reductus predatory mites, which is used to launch a new accumulation system for predatory mites.
  • the density of the Neoseiulus reductus population increases by 1.3-4 times compared to the density that is laid. (Examples - Tables 1.1, 1.3, 1.5).
  • the age structure of populations of predatory and forage ticks (Tables 1.2, 1.4, and 1.6) is characterized by an inverse correlation between the number of eggs and the youngest stages (larvae and nymphs) in the number of adults. These accumulation characteristics make it possible to use Tyreophagus entomophagus as a laboratory host for the accumulation of Neoseiulus reductus in mass quantities for use in plant protection.
  • Neoseiulus reductus during the expansion of the N ° lc production line using Tyreophagus entomophagus
  • Neoseiulus reductusB during the expansion of the Ne 2 production line using Tyreophagus entomophagus
  • the most successfully proposed method can be used for mass breeding of a predatory mite of the Phytoseidae family, the genus Neoseiulus, namely the Neoseiulus reductus species, which is used as biological protection of agricultural crops.
  • the proposed breeding method has high productivity and economic feasibility.
  • the density of the Neoseiulus reductus population increases by 1.3-4 times compared to the density at setting.
  • the productivity of the proposed invention is 5.5 - 6.5 million individuals / m2 of production area. Yu

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Catching Or Destruction (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne un procédé d'élevage d'acariens prédateurs qui consiste à réunir une population d'acariens prédateurs Neoseiulus reductus et une population d'acariens alimentaires Tyreophagus entomophagus, et on donne aux espèces prédatrices la possibilité de se nourrir des espèces alimentaires. On prépare un substrat alimentaire à base de son de blé ou d'écales de sarrasin ou de riz ou de sciure avec une taille des particules de 2-4 mm, qui sont préalablement décontaminées puis disposées dans un conteneur clos avec ventilation; le volume du substrat représente 50% du volume du conteneur; on introduit ensuite dans le conteneur des acariens selon un rapport entre les acariens prédateurs et les acariens alimentaires de 1:5-1:50 des espèces/cm3. Le conteneur est stocké dans un bâtiment à une température de 21-25°C et une humidité de l'air de 40-70% pendant 7-14 jours, et le substrat est mélangé tous les jours. A la fin de la période de culture (7-14 jours), on effectue un comptage de la densité de population des acariens et, en cas de non conformité avec les exigences requises de densité de population, on mélange une partie du substrat avec un nouveau substrat alimentaire et on ajoute des Tyreophagus entomophagus selon un rapport entre les acariens prédateurs et les acariens alimentaires de 1:5-1:50 des espèces/cm3 en fonction du nombre d'acariens prédateurs Neoseiulus reductus que l'on utilise pour lancer un nouveau système d'accumulation d'acariens prédateurs.
PCT/UA2020/000105 2019-12-23 2020-12-09 Procédé d'élevage d'acariens prédateurs neoseiulus reductus WO2021133368A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
UAU201912188 2019-12-23
UAU201912188 2019-12-23

Publications (1)

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WO2021133368A1 true WO2021133368A1 (fr) 2021-07-01

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1830631B1 (fr) * 2004-12-31 2010-05-05 Koppert B.V. Composition d'acariens, son utilisation, procede pour developper un acarien predateur des phytoseiides, systeme de developpement pour developper ledit acarien predateur des phytoseiides et procedes de controle biologique des ravageurs sur une recolte
US20100119645A1 (en) * 2006-08-02 2010-05-13 Syngenta Crop Protection, Inc. Method for rearing predatory mites
ES2533918A1 (es) * 2013-10-11 2015-04-15 Entomotech, S.L. Procedimiento de producción y suelta de ácaros depredadores, composición útil para ello y uso en el control biológico de plagas
RU2617961C2 (ru) * 2012-01-04 2017-04-28 КОППЕРТ Би. Ви. Композиция клещей, содержащая хищного клеща и иммобилизованную жертву, контактирующая с агентом, уничтожающим грибок, и способы и применения, связанные с использованием данной композиции
CN108812553A (zh) * 2018-05-29 2018-11-16 四川农业大学 一种提高加州新小绥螨后代雌性比例的繁育方法
RU2675521C2 (ru) * 2012-01-04 2018-12-19 КОППЕРТ Би. Ви. Композиция клещей, носитель, способ разведения клещей и их применения

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1830631B1 (fr) * 2004-12-31 2010-05-05 Koppert B.V. Composition d'acariens, son utilisation, procede pour developper un acarien predateur des phytoseiides, systeme de developpement pour developper ledit acarien predateur des phytoseiides et procedes de controle biologique des ravageurs sur une recolte
US20100119645A1 (en) * 2006-08-02 2010-05-13 Syngenta Crop Protection, Inc. Method for rearing predatory mites
RU2617961C2 (ru) * 2012-01-04 2017-04-28 КОППЕРТ Би. Ви. Композиция клещей, содержащая хищного клеща и иммобилизованную жертву, контактирующая с агентом, уничтожающим грибок, и способы и применения, связанные с использованием данной композиции
RU2675521C2 (ru) * 2012-01-04 2018-12-19 КОППЕРТ Би. Ви. Композиция клещей, носитель, способ разведения клещей и их применения
ES2533918A1 (es) * 2013-10-11 2015-04-15 Entomotech, S.L. Procedimiento de producción y suelta de ácaros depredadores, composición útil para ello y uso en el control biológico de plagas
CN108812553A (zh) * 2018-05-29 2018-11-16 四川农业大学 一种提高加州新小绥螨后代雌性比例的繁育方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DOKHTORUK ANDREY: "Vspomnim nemnogo o khischnom klesche Neoseiuljuse kukumerise [Let's remember a little about the predatory mite Neoseyulus cuumeris]", ZAKRYTY GRUNT. VSE O TEPLICHNYKH TEKHNOLOGIIAKH I OBORUDOVANII, INTEGRIROVANNOI ZASCHITE RASTENZ, VYRASHCHIVANII OVOSCHNYKH I TSVETOCHNYKH KULTUR [INDOOR GROUND. ALL ABOUT GREENHOUSE TECHNOLOGIES AND EQUIPMENT, INTEGRATED PLANT PROTECTION, GROWING VE, 13 October 2018 (2018-10-13), Russia, pages 1 - 4, XP009529724, Retrieved from the Internet <URL:https://web.archive.org/web/20181022202646/http://info.bio-group.net/zakrytyj-grunt/101-vspomnim-nemnogo-o-khishchnom-kleshche-neosejulyuse-kukumerise> [retrieved on 20210208] *
LEE MING HUI: "Habitat structure and its influence on populations of Amblydromalus limonicus (Acari: Phytoseiidae", SYSTEMATIC AND APPLIED ACAROLOGY, vol. 21, no. 10, 1 January 2016 (2016-01-01), pages 1361 - 1378, XP055834938 *

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