WO2021112349A1 - Préparation microbienne pour décomposer des déchets organiques comprenant la nouvelle souche de geobacillus sp. et un excipient - Google Patents

Préparation microbienne pour décomposer des déchets organiques comprenant la nouvelle souche de geobacillus sp. et un excipient Download PDF

Info

Publication number
WO2021112349A1
WO2021112349A1 PCT/KR2020/006370 KR2020006370W WO2021112349A1 WO 2021112349 A1 WO2021112349 A1 WO 2021112349A1 KR 2020006370 W KR2020006370 W KR 2020006370W WO 2021112349 A1 WO2021112349 A1 WO 2021112349A1
Authority
WO
WIPO (PCT)
Prior art keywords
strain
organic waste
geobacillus
present
waste
Prior art date
Application number
PCT/KR2020/006370
Other languages
English (en)
Korean (ko)
Inventor
전숭종
이주희
정유정
Original Assignee
동의대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020190161856A external-priority patent/KR102283665B1/ko
Priority claimed from KR1020190161850A external-priority patent/KR102175728B1/ko
Application filed by 동의대학교 산학협력단 filed Critical 동의대학교 산학협력단
Publication of WO2021112349A1 publication Critical patent/WO2021112349A1/fr

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
    • B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
    • B09B5/00—Operations not covered by a single other subclass or by a single other group in this subclass
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Definitions

  • the present invention relates to a microorganism preparation for decomposing organic waste comprising a novel strain of the genus Geobacillus and an excipient. More specifically, a method of decomposing organic waste using the decomposition activity of organic wastes of a novel Geobacillus sp. strain isolated from soil, etc., or providing a microbial agent capable of composting organic waste, decomposing or composting organic waste is to provide
  • Food waste (hereafter referred to as food waste) generated daily in Korea is 14,000 tons (Ministry of Environment, as of 2010), accounting for 28.7% of the total amount of waste generated every year. There is a trend of increasing by about 3% (Ministry of Environment, 2014). Food waste is causing enormous economic loss. It not only causes 800 billion won in treatment costs every year, 20 trillion won in economic loss (food resource value) per year, but also greenhouse gas emissions (8.85 million tons of CO2e per year), odor, and food. It is causing major environmental pollution such as water pollution due to wastewater (5.8% of the weight of food waste).
  • Microorganisms currently used for the treatment of organic waste such as food waste in Korea include Actinomycetes, Pseudomonas, Penicillium, Rhizopus, and Pichia.
  • the material decomposition ability is not excellent, so there is still a limit that treatment efficiency is reduced and odors may be generated.
  • a method of using mixed microorganisms including various microorganisms has been proposed as a method to complement the disadvantages of each microorganism, but this is because the conditions under which each microorganism is cultured, for example, pH and temperature, are different from each other.
  • the present invention provides a novel microorganism different from those of prior arts 1 and 2, and has a difference in the effect provided and its activity according to the novel microorganism.
  • Another object of the present invention is to provide a method for treating organic waste using the microbial agent.
  • Another object of the present invention is to provide a method for composting organic waste using the microbial agent.
  • the microorganism preparation for decomposing organic waste is to include a Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain (accession number KCTC13853BP) and an excipient.
  • a Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain (accession number KCTC13853BP) and an excipient.
  • the strain may have at least one enzyme activity selected from the group consisting of amylase, protease, and lipase.
  • the strain may be salt-tolerant.
  • the strain may have an optimum growth temperature of 30 to 80° C. and an optimum growth pH of 5 to 9.
  • the organic waste may be food waste or manure.
  • the manure is defined as including human or animal excrement.
  • the microorganism preparation for composting of organic waste is to include a Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain (Accession No. KCTC13853BP), an excipient and a plant mixture.
  • a Geobacillus genus JH-8 Geobacillus sp. JH-8 strain (Accession No. KCTC13853BP)
  • an excipient and a plant mixture.
  • the organic waste may be food waste or manure.
  • the method for composting organic waste according to another embodiment of the present invention may include treating the microbial agent to the organic waste.
  • the method of treating organic waste according to another embodiment of the present invention may include treating the microbial agent to the organic waste.
  • the microorganism preparation for decomposing organic waste is to include a Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain (accession number KCTC13853BP), an excipient and a plant mixture.
  • a Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain (accession number KCTC13853BP)
  • an excipient accession number KCTC13853BP
  • the strain of the present invention is a novel strain of the genus Geobacillus JH-8, accession number KCTC13853BP.
  • the Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain (Accession No.: KCTC13853BP) of the present invention may include 16s rRNA represented by SEQ ID NO: 1.
  • the novel strain of the present invention can grow well even at a high temperature of 50 ° C or higher among samples collected from soil around Gyeongsangnam-do, and has an enzyme capable of decomposing all of proteins, carbohydrates and fats, and at the same time in the process of decomposing organic waste.
  • a Gram-positive bacillus having the characteristic that it does not contain a malodor generating enzyme that may be generated, it was identified as a Geobacillus sp. strain through phylogenetic analysis using the nucleotide sequence of 16s rRNA represented by SEQ ID NO: 1.
  • the decomposition of organic waste of the present invention is a pretreatment that not only completely decomposes organic waste itself, but also facilitates incineration or landfill by hydrolyzing the binding of specific components of organic waste, for example, protein, fat and carbohydrate This means that all processes are included.
  • the organic waste of the present invention is, for example, food waste, sludge from sewage wastewater treatment plant, waste from aquatic products processing plant, waste from agricultural and livestock processing plant, waste from food paper mill, slaughter waste from slaughterhouse, livestock manure and other organic materials If it is a waste that includes, it may be included without being limited thereto.
  • the organic waste of the present invention may be food waste or manure, but is not limited thereto.
  • the manure of the present invention may be human or livestock manure, but is not limited thereto.
  • the microorganism preparation of the present invention may be in the form of a solid or liquid.
  • the moisture content is dried to 0.1 to 10% by weight and commercialized in the form of beads, or it can be pulverized and commercialized in powder form.
  • the Geobacillus genus JH-8 strain (accession number KCTC13853BP) is inoculated into the medium and cultured, and the obtained culture and the carrier are mixed and then dried through the process of drying the obtained microorganism fixation and microorganism It can be manufactured through the process of mixing nutrients, which are food ingredients of
  • any medium can be used as long as it is a medium capable of growth of the strain, and microbial nutrients commonly included in water, for example, yeast extract, glucose, sodium chloride, bovine extract, etc. may be used. .
  • the culture time of the strain of the present invention may be 12 hours to 48 hours, but is not limited thereto.
  • any known carrier for preparing a microbial agent may be used, for example, a vegetable carrier such as sawdust, rice husk, bran or powdery clay, activated carbon, coke, volcanic ash and combustion ash. At least one selected from the group may be used, and as the clay, at least one selected from the group consisting of zeolite, vermiculite, diatomaceous earth, kaolin, porcelain, feldspar, chard and talc may be used, but is not limited thereto. .
  • the adsorption property of the microbial agent can be increased, and it has the advantage that it can have a buffering ability for pH and can exhibit an adsorption effect on odor substances.
  • Various nutrients such as glucose, molasses, soy flour, sesame cake, fish meal, etc. may be used as the food component nutrients used in the microbial preparation of the present invention.
  • the nutrients may be mixed with the microbial fixation in a volume ratio of 0.2 to 1:10, but the present invention is not limited thereto.
  • the Geobacillus genus JH-8 strain (Accession No. KCTC13853BP) of the present invention has the advantage that it is not limited in nutrients because it can grow by decomposing various nutrients.
  • the microbial preparation of the present invention may further include an excipient.
  • the excipient of the present invention may be at least one selected from the group consisting of illite, activated carbon and elvan, but is not limited thereto. More preferably, the excipient may be included in a weight ratio of 0.1 to 5 times with respect to the microbial fixation, but is not limited thereto.
  • the microbial preparation of the present invention may further include a cryoprotectant.
  • the cryoprotectant may be glycerol, skim milk, honey, or the like, but is not limited thereto.
  • the microorganism preparation of the present invention is liquid, it may be used by mixing the culture of the microorganism and mixing glucose or glycerin to stabilize the microorganism, but is not limited thereto.
  • the microbial preparation of the present invention further comprises a plant mixture preparation, and the plant mixture preparation is Schisandra extract; calamus extract; eggplant leaf extract; dandelion extract; It may include any one selected from the group consisting of juniper extract and mixtures thereof.
  • the activity of the microorganisms is enhanced, so that the decomposition activity for organic wastes such as food waste and manure including human and animal excrement is increased, and the effect of removing odors can be increased.
  • the plant mixture includes Schisandra extract, 1 to 10 parts by weight of iris extract, 1 to 10 parts by weight of eggplant leaf extract, 0.5 to 5 parts by weight of dandelion extract, and 0.1 parts by weight of juniper extract based on 100 parts by weight of Schisandra extract. to 2 parts by weight.
  • the effect of promoting the activity of the microorganism is the most excellent, and thus the decomposition activity of organic waste and the effect of rapidly removing the odor may be the best.
  • the strain may have at least one enzymatic activity selected from the group consisting of amylase, protease and lipase, preferably amylase, protease and lipase. , but is not limited thereto.
  • the amylase of the present invention is a generic term for enzymes that hydrolyze starch, and is widely distributed in nature from higher plants to microorganisms, and hydrolyzes ⁇ -1,4-linkage or ⁇ -1,6-linkage of starch. has the function to Specifically, the amylase of the present invention is ⁇ -amylase ( ⁇ -1,4-glucan-4-glucano hydrolase), ⁇ -amylase ( ⁇ -1,4-glucan maltohydrolase) and ⁇ -amylase (amyllo glucosidase) may be at least one selected from the group consisting of, but is not limited thereto.
  • protease of the present invention is a generic term for enzymes that hydrolyze peptide bonds of proteins or peptides, and is also referred to as proteolytic enzymes.
  • the lipase of the present invention hydrolyzes triglycerides in a suspension of lipids and water to form monoglycerides, glycerol, and free fatty acids, as well as ester synthesis or exchange reaction from alcohols and fatty acids also catalyzes Since the lipase was first discovered in animal pancreas, it was found to be present in animal organs and plant seeds, and it was also found to be derived from microorganisms such as fungi and bacteria, confirming that it is widely distributed throughout the biological world. Of these, exocrine lipases produced by microorganisms have more heat stability than lipases derived from animals and plants, and thus can be applied for industrial purposes.
  • the strain may be salt-tolerant.
  • the strain of the present invention has the advantage of being able to decompose all proteins, carbohydrates, and fats while at the same time being resistant to salt, so that it can grow very effectively even in an environment in which food waste and manure containing a large amount of salt exist. .
  • the strain may have an optimum growth temperature of 30 to 80° C. and an optimum growth pH of 5 to 9.
  • the optimal growth temperature is 50 to 75 °C
  • the optimal growth pH may be 5.5 to 7
  • more preferably the optimal growth temperature is 70 °C
  • the optimal growth pH may be 6, but is not limited thereto. If the optimum growth temperature is less than the minimum value, the strain may not grow sufficiently, and if the optimum growth temperature exceeds the maximum value, the strain may be destroyed at high temperature. In addition, when it exceeds the minimum and maximum values of the optimal growth pH, the strain may be destroyed.
  • the strain of the present invention is composed of many organic substances such as carbohydrates, proteins, and fats, and the food waste, which accounts for most of the organic waste in Korea, has a pH of 4.0 to pH 6.5, so it very effectively decomposes the organic matter in the food waste. It can be applied very efficiently to the industrial fields of fermentation and extinction.
  • the present invention provides a culture of Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain (Accession No. KCTC13853BP).
  • culture used in the present invention means a product obtained by culturing a microorganism in a known liquid medium or solid medium, and is a concept including microorganisms.
  • the culture is a product obtained after culturing the strain of the present invention in a medium
  • the medium may be selected from a known liquid medium or solid medium used for culturing the strain of the genus Geobacillus.
  • the present invention provides a culture solution from which the strain is removed from the culture of the genus Geobacillus JH-8 (Geobacillus sp. JH-8) (Accession No. KCTC13853BP).
  • culture solution refers to a product obtained by culturing microorganisms in a known liquid medium or solid medium, and is a concept that does not include microorganisms.
  • the culture is a product obtained after culturing the strain of the present invention in a medium
  • the medium may be selected from a known liquid medium or solid medium used for culturing the strain of the genus Geobacillus.
  • the organic waste may be food waste or manure.
  • the microorganism preparation for composting of organic waste is to include a Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain (Accession No. KCTC13853BP), an excipient and a plant mixture.
  • a Geobacillus genus JH-8 Geobacillus sp. JH-8 strain (Accession No. KCTC13853BP)
  • an excipient and a plant mixture.
  • Composting of organic waste means composting which can fertilize the soil by hydrolyzing the bonds of certain components of the organic waste, such as proteins, fats and carbohydrates.
  • the organic waste may be food waste or manure.
  • the method for composting organic waste according to another embodiment of the present invention may include treating the microbial agent to the organic waste.
  • the information regarding the Geobacillus sp. JH-8 strain is the same as described in the strain and microbial preparation, so excessive complexity of the specification is reduced omitted to avoid
  • the method of treating organic waste according to another embodiment of the present invention may include treating the microbial agent to the organic waste.
  • the content regarding the Geobacillus sp. JH-8 strain (Accession No. KCTC13853BP) is the same as described in the strain and microbial preparation, and excessive complexity of the specification is reduced omitted to avoid
  • the novel Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain of the present invention (accession number: KCTC13853BP) is capable of decomposing all proteins, carbohydrates and fats while at the same time has resistance to salt, so it contains a large amount of salt. It can grow very effectively even in food waste and manure that contain Furthermore, such a strain can reduce costs because the culture process is relatively simple, and there is an advantage of being environmentally friendly compared to the conventional chemical decomposition method.
  • 1 is a graph showing the optimum growth pH range of a novel strain according to an embodiment of the present invention.
  • Figure 2 is a graph showing the optimal growth temperature range of the novel strain according to an embodiment of the present invention.
  • FIG. 3 shows the results of genotyping using 16s rRNA of a novel strain according to an embodiment of the present invention.
  • the present invention relates to a microbial preparation for decomposing organic waste, including a Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain (Accession No. KCTC13853BP), an excipient and a plant mixture.
  • a Geobacillus genus JH-8 Geobacillus sp. JH-8 strain (Accession No. KCTC13853BP)
  • an excipient and a plant mixture.
  • Strains that do not lose their activity even at 50° C. or higher were selected through the following procedure. Specifically, 1 g of various fungal sources samples collected from common soil, humus, and compost around Gyeongsangnam-do were suspended in 10 ml of 0.85% NaCl solution. Then, the suspension was diluted 10-fold, 100-fold, and 1000-fold to contain a nutrient medium (beef extract 3%, yeast extract 5%, agar 1.8%, pH 7.0). Plates were plated and incubated at 50 °C for 24 hours.
  • a nutrient medium beef extract 3%, yeast extract 5%, agar 1.8%, pH 7.0
  • Example 1 In order to confirm the growth of the strain, the strain of Example 1 was smeared on a plate containing a nutrient broth, incubated at 50 ° C. for 12 hours, and the growth rate according to the degree of colony formation was confirmed. The results are shown in Table 1 below.
  • the strain selected in Example 1 was inoculated on a plate containing a nutrient plate medium and cultured overnight at 50 °C. Then, iodine was treated on the plate in which the cultured strain is present, and the generation of a clear zone was checked, and the results are shown in Table 1 below.
  • 1% soluble starch in the case of amylase, 1% skim milk in the case of protease, and 1% tributyrin in the case of lipase were added to the nutritional plate medium.
  • Example 2 API kit (Biomerieux, USA) and morphology analysis were performed on the form of A-3 having all three enzyme activities, and the results are shown in Table 2 below.
  • the pH in the medium of Example 1 was adjusted to a range of 5.0 to 9.0, and the temperature was adjusted to a range of 30 to 80 ° C., and A-3 was cultured. Subsequently, the cell mass was measured, and the results are shown in FIGS. 2 and 3 .
  • A-3 was aerobic, and it was confirmed that the colony shape on the solid medium was round, the color was white, and the surface was in contact and smooth.
  • the A-3 is a gram-positive bacillus, has no flagella, formed endospores, did not produce H2S, urease, tryptophane deaminase, etc., and glucose, mannitol ( Various sugars such as mannitol) and sucrose were available.
  • the viable pH of A-3 was 5.0 to 9.0, and the temperature was 30 to 75 °C.
  • A-3 according to the present invention does not have an enzyme that can cause odor when decomposing organic matter, and can maintain growth by various nutrient sources, as well as organic waste, especially food waste. It can be seen that the organic waste treatment efficiency can be remarkably increased because it can be effectively propagated even when it contains a very high level of salt.
  • Chromosomes were extracted from the A-3 strain using the GENExTM genomic kit (General Biosystem, Korea). After mixing the extracted chromosome with Taq DNA polymerase (Takara shuzo, Japan) and the primers of SEQ ID NOs: 2 and 3, and performing Polymerase Chain Reaction to amplify 16s rRNA of strain A-3, , The nucleotide sequence was analyzed using an ABI PRISM 310 genetic analyzer (Perkin-Elmer Applied Biosystems, USA). In addition, in the case of homology analysis, the NCBI BLAST program was used, and the results are shown in FIG. 1 .
  • strain A-3 has the 16s rRNA sequence of SEQ ID NO: 1, and exhibits the highest 99% sequence homology with Geobacillus zalihae NBRC 101842.
  • A-3 was identified as a novel Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain belonging to the genus Geobacillus. and deposited it with the Korea Research Institute of Bioscience and Biotechnology (Accession No.: KCTC13853BP).
  • a microbial preparation (JH8) for decomposing organic waste was prepared using the Geobacillus genus JH-8 (Geobacillus sp. JH-8) strain.
  • the time it takes for the microbial preparation to be treated with the following livestock manure (A) and food waste (B) and the odor is removed (decomposition rate 1, E1) and the time it takes for the weight to be reduced by half ( The degradation rate 2, E2) was evaluated.
  • Plant extracts as shown in [Table 4] below were prepared in order to evaluate the degree of enhancement of decomposition activity for organic wastes by mixing plant mixtures with respect to the microbial preparations.
  • Schisandra extract (S), iris extract (T), eggplant leaf extract (U), dandelion extract (X), and juniper extract (Z) were prepared by hot water extraction and filtration of each plant material.
  • the plant extract was mixed as shown in Table 4 below and mixed with the microbial preparation to prepare a microbial preparation for decomposing organic waste.
  • M1 M2 M3 M4 M5 M6 M7 M8 M9 S 100 100 100 100 100 100 T 100 0.1 One 5 10 15 U 100 0.1 One 5 10 15 X 100 0.1 0.5 3 5 7 Z 0.01 0.1 One 2 3
  • the prepared microbial preparation was treated in the manure (A), and the degradation rate 1 (E1) and degradation rate 2 (E2) were evaluated in the same manner as in the above-described method.
  • the index was fixed to 10 as a control when only a microbial preparation was used without using a plant mixture preparation, Comparative evaluation of examples in which the plant mixture preparation was mixed was evaluated as an index The results are shown. In the index, the lower the number, the better the effect.
  • the decomposition activity of microorganisms can be increased when the microorganisms are mixed with a plant extract of a certain range with respect to the preparation and used as a plant mixture preparation.
  • the present invention relates to a microorganism preparation for decomposing organic waste comprising a novel strain of the genus Geobacillus and an excipient. More specifically, a method of decomposing organic waste using the decomposition activity of organic wastes of a novel Geobacillus sp. strain isolated from soil, etc., or providing a microbial agent capable of composting organic waste, decomposing or composting organic waste is to provide

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Processing Of Solid Wastes (AREA)
  • Treatment Of Sludge (AREA)

Abstract

La présente invention concerne une préparation microbienne pour décomposer des déchets organiques, comprenant une nouvelle souche de Geobacillus sp. et un excipient et, plus spécifiquement, une préparation microbienne pour décomposer des déchets organiques, comprenant une nouvelle souche de Geobacillus sp. ayant une très forte activité de décomposition de déchets organiques, un procédé de décomposition de déchets organiques l'utilisant, ainsi qu'un procédé de compostage de déchets organiques. La souche peut décomposer tous les protéines, glucides et graisses et est, en même temps, résistante au sel, peut donc être très efficacement cultivée même dans les excréments et les déchets alimentaires contenant une grande quantité de sel. De plus, la souche ne comprend pas d'enzyme qui génère de mauvaises odeurs lors de la décomposition de matière organique et peut ainsi être très appropriée pour une utilisation dans la décomposition de déchets organiques.
PCT/KR2020/006370 2019-12-06 2020-05-14 Préparation microbienne pour décomposer des déchets organiques comprenant la nouvelle souche de geobacillus sp. et un excipient WO2021112349A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2019-0161850 2019-12-06
KR10-2019-0161856 2019-12-06
KR1020190161856A KR102283665B1 (ko) 2019-12-06 2019-12-06 신규한 균주인 지오바실러스속 균주 및 식물 혼합제제를 포함하는 유기성 폐기물 분해용 미생물 제제
KR1020190161850A KR102175728B1 (ko) 2019-12-06 2019-12-06 신규한 지오바실러스속 균주 및 부형제를 포함하는 유기성 폐기물 분해용 미생물 제제

Publications (1)

Publication Number Publication Date
WO2021112349A1 true WO2021112349A1 (fr) 2021-06-10

Family

ID=76222105

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2020/006370 WO2021112349A1 (fr) 2019-12-06 2020-05-14 Préparation microbienne pour décomposer des déchets organiques comprenant la nouvelle souche de geobacillus sp. et un excipient

Country Status (1)

Country Link
WO (1) WO2021112349A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050086014A (ko) * 2003-08-27 2005-08-30 가부시키가이샤 신꼬간꾜우솔루션 신규 미생물 및 그 미생물을 사용한 유기성 고형물의 처리방법
JP2006238794A (ja) * 2005-03-03 2006-09-14 Tokyo Gas Co Ltd 新規微生物、当該新規微生物を用いた廃水処理方法及び廃水処理装置
KR100807275B1 (ko) * 2006-12-22 2008-02-28 목원대학교 산학협력단 초고온성 단백질분해효소를 생산하는 지오바실러스카우스토필러스 ｃ-2 균주 및 그의 이용방법
KR20090126917A (ko) * 2008-06-05 2009-12-09 한경대학교 산학협력단 질산염제거 기능을 가지는 새로운 지오바실러스디모디니트리피컨스 ｓｇ-01 균주 및 그의 이용방법
KR20110002512A (ko) * 2009-07-02 2011-01-10 학교법인 동의학원 고온성 리파제를 생산하는 지오바실러스 속 ｈｗ1 균주 및 이의 용도
KR102055544B1 (ko) * 2019-06-11 2019-12-13 동의대학교 산학협력단 신규한 지오바실러스속 균주 및 이의 용도

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050086014A (ko) * 2003-08-27 2005-08-30 가부시키가이샤 신꼬간꾜우솔루션 신규 미생물 및 그 미생물을 사용한 유기성 고형물의 처리방법
JP2006238794A (ja) * 2005-03-03 2006-09-14 Tokyo Gas Co Ltd 新規微生物、当該新規微生物を用いた廃水処理方法及び廃水処理装置
KR100807275B1 (ko) * 2006-12-22 2008-02-28 목원대학교 산학협력단 초고온성 단백질분해효소를 생산하는 지오바실러스카우스토필러스 ｃ-2 균주 및 그의 이용방법
KR20090126917A (ko) * 2008-06-05 2009-12-09 한경대학교 산학협력단 질산염제거 기능을 가지는 새로운 지오바실러스디모디니트리피컨스 ｓｇ-01 균주 및 그의 이용방법
KR20110002512A (ko) * 2009-07-02 2011-01-10 학교법인 동의학원 고온성 리파제를 생산하는 지오바실러스 속 ｈｗ1 균주 및 이의 용도
KR102055544B1 (ko) * 2019-06-11 2019-12-13 동의대학교 산학협력단 신규한 지오바실러스속 균주 및 이의 용도

Similar Documents

Publication Publication Date Title
KR102004089B1 (ko) 음식물쓰레기 분해 혼합균주 및 이를 이용한 음식물쓰레기 분해 방법
AU2021103470A4 (en) Bacillus subtilis bs40-4 strain and method for composting organic wastes by using the same
WO2011078601A9 (fr) Culture de souches mélangées pour élimination de déchets de cuisine, et procédé d'élimination de déchets de cuisine utilisant cette culture de souches mélangées
KR100664730B1 (ko) 토양개량 및 환경 개선용 미생물제제의 제조방법 및 상기방법에 의해 제조한 미생물제제
CN103667155B (zh) 一株枯草芽孢杆菌Bacillus subtilis 3-2及其应用
CN103695354B (zh) 一株蜡状芽孢杆菌Bacillus cereus J2及其应用
KR20140119850A (ko) 바이오 플락 양식시스템의 수질 정화를 위한 염분 내성을 갖는 바실러스 속 신규 균주를 이용한 미생물 제재
CN114574383B (zh) 高效厨余垃圾降解复合微生物菌剂及其制备方法与应用
CN114540252B (zh) 一种转化畜禽养殖废弃物的微小杆菌p6及应用
WO2020251113A1 (fr) Nouveau geobacillus sp. souche et utilisation associée
CN113481130A (zh) 一种耐高温餐厨垃圾腐熟复合菌剂及其制备方法和应用
Bhimani et al. Cultivation methods, characterization, and biocatalytic potential of organic solid waste degrading bacteria isolated from sugarcane rhizospheric soil and compost
KR102175728B1 (ko) 신규한 지오바실러스속 균주 및 부형제를 포함하는 유기성 폐기물 분해용 미생물 제제
Ramanathan et al. Isolation, characterization and role of gut bacteria of three different millipede species
WO2021112349A1 (fr) Préparation microbienne pour décomposer des déchets organiques comprenant la nouvelle souche de geobacillus sp. et un excipient
CN107384799A (zh) 降解羽毛菌株的筛选方法及其在降解羽毛方面的应用方法
KR101455925B1 (ko) 양식장 폐수 처리에 적합한 갯지렁이 유래 바실러스 속 신규 균주 및 미생물 제재
RU2291900C2 (ru) Гипертермофильный штамм caldothrix satsumae, способный ферментировать органические отходы при высокой температуре
KR101476031B1 (ko) 복수 종의 바실러스 혼합균에 의한 염분을 함유한 유기폐수 정화방법 및 미생물제재
CN114921356A (zh) 家用型厨余垃圾好氧堆肥复合微生物菌剂及其制备方法
KR102283665B1 (ko) 신규한 균주인 지오바실러스속 균주 및 식물 혼합제제를 포함하는 유기성 폐기물 분해용 미생물 제제
KR20140119847A (ko) 갯지렁이 유래 바실러스 속 신규 균주를 이용한 바이오 플락 양식시스템의 수질 정화용 미생물 제재
KR20110040229A (ko) 바실러스 서브틸리스 ｃｂｓ 및 이를 포함하는 생균제
KR102581363B1 (ko) 신규 바실러스 스미시 ep184 균주 및 그의 용도
Kantha et al. Synergistic growth of lactic acid bacteria and photosynthetic bacteria for possible use as a bio-fertilizer

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20895417

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 20895417

Country of ref document: EP

Kind code of ref document: A1