WO2021075655A1 - Amorce et sonde pour la détection simultanée de mycoplasma pneumoniae et de mutation de mycoplasma pneumoniae et utilisations associées - Google Patents

Amorce et sonde pour la détection simultanée de mycoplasma pneumoniae et de mutation de mycoplasma pneumoniae et utilisations associées Download PDF

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Publication number
WO2021075655A1
WO2021075655A1 PCT/KR2020/006513 KR2020006513W WO2021075655A1 WO 2021075655 A1 WO2021075655 A1 WO 2021075655A1 KR 2020006513 W KR2020006513 W KR 2020006513W WO 2021075655 A1 WO2021075655 A1 WO 2021075655A1
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mycoplasma
pneumococcus
antibiotic
wild
composition
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PCT/KR2020/006513
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English (en)
Korean (ko)
Inventor
임채승
장웅식
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고려대학교 산학협력단
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Publication of WO2021075655A1 publication Critical patent/WO2021075655A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Definitions

  • the present invention can accurately and quickly detect mycoplasma, which is the causative agent of pneumonia, and can detect antibiotic-sensitive and resistant mycoplasma pneumococcus by discriminating.
  • the composition of the present invention can detect Mycoplasma pneumococcus simply by using PCR, and the composition of the present invention has high sensitivity and specificity for the detection of Mycoplasma pneumococcus. Can be used for diagnosis.
  • FIG. 6 is a diagram showing the mycoplasma detection result of a primer and probe set using a positive clinical sample.
  • Table 8 shows the Ct values of the mycoplasma primer set prepared in Example 1 for two clinical samples
  • Table 9 shows the Ct values of the mycoplasma reference primer
  • Table 10 shows 10 mycoplasma negative It shows the comparison of the Ct values of the mycoplasma primer set and the reference primer designed in Example 1 for the sample.
  • One of the two clinical samples was identified as wild-type and the other was identified as a 2064G mutant. As a result of confirming by sequencing, it was accurately reconfirmed with wild-type and 2063G mutant (FIGS. 6 and 7). Both reference primers showed positive results, but it took significantly more time to present the results compared to the primer set of the present invention.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne une composition qui comprend une amorce et une sonde et par lesquelles une mycoplasma pneumoniae et des mutations de mycoplasma pneumoniae peuvent être simultanément discernées et détectées. Une composition selon la présente invention peut être utilisée pour détecter avec rapidité et précision un mycoplasme, qui est l'agent causal de la pneumonie, et discerner et détecter une pneumonie à mycoplasme sensible aux antibiotiques et résistante aux antibiotiques, et peut ainsi être utilisé pour un diagnostic clinique sur site nécessitant un diagnostic rapide de l'agent causal de la pneumonie.
PCT/KR2020/006513 2019-10-18 2020-05-19 Amorce et sonde pour la détection simultanée de mycoplasma pneumoniae et de mutation de mycoplasma pneumoniae et utilisations associées WO2021075655A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2019-0129648 2019-10-18
KR1020190129648A KR102250376B1 (ko) 2019-10-18 2019-10-18 마이코플라즈마 폐렴균 및 마이코플라즈마 폐렴균 돌연변이 동시 검출용 프라이머와 프로브 및 이의 용도

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WO2021075655A1 true WO2021075655A1 (fr) 2021-04-22

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070069086A (ko) * 2005-12-27 2007-07-02 삼성전자주식회사 9 종의 호흡기 질환을 야기시키는 박테리아의 표적 서열을증폭시킬 수 있는 프라이머 세트, 9종의 박테리아의 표적서열에 특이적으로 혼성화하는 프로브 세트, 상기 프로브세트가 고정화되어 있는 마이크로어레이 및 상기 프로브세트를 이용하여 9종의 박테리아의 존재를 검출하는 방법
CN104630328A (zh) * 2013-11-08 2015-05-20 江苏默乐生物科技有限公司 肺炎支原体23S rRNA 2064位点A:G突变检测特异性引物和探针
CN104630329A (zh) * 2013-11-08 2015-05-20 江苏默乐生物科技有限公司 肺炎支原体23S rRNA 2063位点A:G突变检测特异性引物和探针
JP2017148008A (ja) * 2016-02-26 2017-08-31 東ソー株式会社 プライマー及びマイコプラズマ・ニューモニエの検出方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070069086A (ko) * 2005-12-27 2007-07-02 삼성전자주식회사 9 종의 호흡기 질환을 야기시키는 박테리아의 표적 서열을증폭시킬 수 있는 프라이머 세트, 9종의 박테리아의 표적서열에 특이적으로 혼성화하는 프로브 세트, 상기 프로브세트가 고정화되어 있는 마이크로어레이 및 상기 프로브세트를 이용하여 9종의 박테리아의 존재를 검출하는 방법
CN104630328A (zh) * 2013-11-08 2015-05-20 江苏默乐生物科技有限公司 肺炎支原体23S rRNA 2064位点A:G突变检测特异性引物和探针
CN104630329A (zh) * 2013-11-08 2015-05-20 江苏默乐生物科技有限公司 肺炎支原体23S rRNA 2063位点A:G突变检测特异性引物和探针
JP2017148008A (ja) * 2016-02-26 2017-08-31 東ソー株式会社 プライマー及びマイコプラズマ・ニューモニエの検出方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE Nucleotides GenBank; ANONYMOUS: "Mycoplasma pneumoniae gene for 23S ribosomal RNA gene domain V, partial sequence, isolate: Y935", XP055801966, retrieved from NCBI *

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KR102250376B1 (ko) 2021-05-12
KR102250376B9 (ko) 2022-01-17
KR20210046888A (ko) 2021-04-29

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