WO2021073381A1 - 微流控基板及其流体驱动方法、微流控装置 - Google Patents

微流控基板及其流体驱动方法、微流控装置 Download PDF

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Publication number
WO2021073381A1
WO2021073381A1 PCT/CN2020/117024 CN2020117024W WO2021073381A1 WO 2021073381 A1 WO2021073381 A1 WO 2021073381A1 CN 2020117024 W CN2020117024 W CN 2020117024W WO 2021073381 A1 WO2021073381 A1 WO 2021073381A1
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fluid
volume
port
quantitative
microfluidic substrate
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PCT/CN2020/117024
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English (en)
French (fr)
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胡立教
袁春根
崔皓辰
胡涛
申晓贺
李婧
甘伟琼
Original Assignee
京东方科技集团股份有限公司
北京京东方健康科技有限公司
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Publication of WO2021073381A1 publication Critical patent/WO2021073381A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution

Definitions

  • the present disclosure relates to the field of biological detection, and more particularly to a microfluidic substrate and a fluid driving method thereof, and a microfluidic device.
  • the microfluidic device is also called Lab-on-a-chip, which refers to the integration of basic operation units such as sample preparation, reaction, separation, and detection involved in the fields of biology, chemistry, and medicine. The whole process of reaction and analysis is automatically completed on the micrometer-scale microchannel chip.
  • the advantages of the analysis and detection device based on the microfluidic device are: less sample volume, fast analysis speed, easy to make a portable instrument, and very suitable for real-time, on-site analysis.
  • it is necessary to integrate as many functions as possible, such as reaction and analysis, into the device, so as to reduce the dependence on operations outside the device.
  • the microfluidic device can be designed as a one-time use product, which can save complicated cleaning and waste liquid treatment liquid path systems.
  • an embodiment of the present disclosure provides a microfluidic substrate, including: a first quantitative portion having a first volume and configured to provide a first volume equal to the first volume The first fluid.
  • the microfluidic substrate further includes a first feeding part, a second feeding part, and a first discharging part, wherein the first dosing part includes a first port and a second port.
  • a quantitative portion is in fluid communication with the first feeding portion through the first port and is in fluid communication with the second feeding portion and the first discharge portion through the second port, respectively.
  • the first feeding part is configured to provide a first fluid larger than the first volume to the first dosing part through the first port, and the second feeding part is configured To provide a second fluid.
  • the microfluidic substrate further includes a second quantitative portion for quantitatively controlling the volume of the mixture of the first fluid and the second fluid, and the second quantitative portion includes a membrane pump cavity having a second volume.
  • the membrane pump cavity includes an elastic membrane and is configured to quantify the second volume by controlling the amount of deformation of the elastic membrane.
  • the second dosing part includes a third port and a fourth port, and the third port is in fluid communication with the first port.
  • the microfluidic substrate further includes a third quantitative part having a third volume and configured to quantitatively control the volume of the fluid in the third quantitative part.
  • the third quantitative portion includes a detection site and the third quantitative portion includes a fifth port and a sixth port, and the fifth port is in fluid communication with the fourth port.
  • the microfluidic substrate further includes a second discharge part, the second discharge part is in fluid communication with the sixth port and is configured to contain fluid flowing out of the third dosing part.
  • the microfluidic substrate further includes a main body and a first covering member, wherein the main body and the first covering member are attached to form the first quantitative portion, the first feeding portion, and the first covering member.
  • the first covering member includes a composite structure of a lyophilic layer and an elastic layer.
  • the microfluidic substrate further includes a second covering member located on a side of the main body part away from the first covering member, and the second covering member is used to protect the first feeding part and the second One or more of the feeding part, the first discharging part, and the second discharging part are sealed.
  • an embodiment of the present disclosure also provides a microfluidic device, including the above-mentioned microfluidic substrate.
  • an embodiment of the present disclosure also provides a fluid driving method for a microfluidic substrate, including: driving a first fluid to flow through a first port to fill a first quantitative portion, and during the filling, the excess second A fluid flows into the first discharge portion; wherein the first quantitative portion has a first volume and is configured to provide a first fluid of a first volume equal to the first volume.
  • the fluid driving method further includes, after driving the first fluid to flow through the first port to fill the first dosing part: driving the second fluid to flow through the second port to enter the first dosing part, At the same time, the first fluid inside the first dosing part then flows through the first port to the second dosing part.
  • the fluid driving method further includes, after driving the second fluid to flow through the second port to enter the first dosing part: driving the first fluid and the second fluid into the second dosing part until The mixture of the first fluid and the second fluid reaches a predefined volume in the second dosing part, wherein the second dosing part includes a membrane pump cavity with a second volume, and the membrane pump cavity includes an elastic membrane and is configured To quantify the second volume by controlling the amount of deformation of the elastic membrane.
  • the fluid driving method further includes, after the mixture of the first fluid and the second fluid reaches a predefined volume in the second quantitative part: driving the elastic membrane by changing the amount of deformation of the elastic membrane.
  • the mixture passes through the fourth port to fill the third dosing part, and the fluid flowing out of the third dosing part flows into the second discharge part during the filling; and detecting the mixture in the third dosing part, wherein the The third quantitative part has a third volume and is configured to quantitatively control the volume of the mixture in the third quantitative part.
  • the first quantitative part can be used to accurately control the first fluid (for example, the sample to be detected) without a flow sensor.
  • the volume of the first fluid is reduced, and the volume of the first fluid is reduced.
  • the structure is simple and the operation is convenient.
  • the second quantitative part including the elastic membrane can be used to accurately control the volume of the mixture (for example, the diluted sample) of the first fluid and the second fluid (for example, the diluent), and the structure Simple and easy to operate.
  • the embodiments of the present disclosure can realize operations such as quantitative transportation, dilution, mixing, and reaction of fluids, without complicated external sensors and fluid drive control devices, improving the integration of the system, and reducing the process complexity and cost of the system.
  • the second cover is used as a consumable component, which reduces the risk of sample contamination and biological exposure, and eliminates complicated cleaning steps.
  • Fig. 1 is a schematic structural diagram of a microfluidic substrate according to an embodiment of the present disclosure
  • FIG. 2 is a schematic cross-sectional view of the second quantitative part taken along the line A-B in FIG. 1;
  • FIG. 3 is a schematic diagram of the structure of the main body of the microfluidic substrate according to an embodiment of the present disclosure
  • FIG. 4 is a schematic structural diagram of a second covering member of a microfluidic substrate according to an embodiment of the present disclosure
  • Fig. 5 is a schematic structural diagram of a fastener of a microfluidic substrate according to an embodiment of the present disclosure
  • Fig. 6 is a schematic structural diagram of a first cover of a microfluidic substrate according to an embodiment of the present disclosure
  • FIG. 7 is a schematic diagram of the structure of the bonding portion of the microfluidic substrate according to an embodiment of the present disclosure.
  • FIG. 8 is a schematic perspective view of a microfluidic substrate according to an embodiment of the present disclosure.
  • FIG. 9 is a flowchart of a fluid driving method according to an embodiment of the present disclosure.
  • An object of the present disclosure is to develop a microfluidic substrate, a microfluidic device, and a fluid driving method for the microfluidic substrate that perform fluid quantification without using a fluid flow sensor.
  • Figure 1 depicts a schematic structural diagram of a microfluidic substrate based on an embodiment of the present disclosure.
  • the fluid space 100 of the microfluidic substrate includes a first quantitative portion 110.
  • the first dosing part 110 has a first volume and is configured to provide a first volume of a first fluid equal to the first volume.
  • the first volume (that is, the first volume) V1 is calculated according to formula (1):
  • V1 L1*W1*D1 (1)
  • L1 is the length of the cuboid
  • W1 is the width of the cuboid
  • D1 is the height or depth of the cuboid.
  • the fluid space 100 of the microfluidic substrate further includes a first feeding part 120 for adding a first fluid to the fluid space, a second feeding part 130 for adding a second fluid to the fluid space, and a certain amount of The first discharge part 140 of the fluid.
  • the first quantitative portion 110 includes a first port 112 and a second port 114.
  • the first dosing part 110 is in fluid communication with the first feeding part 120 through the first port 112 and is in fluid communication with the second feeding part 130 and the first discharging part 140 through the second port 114, respectively.
  • the first feeding part 120 is configured to provide the first dosing part 110 with a first fluid having a volume greater than the first volume V1 through the first port 112, and the second feeding part 130 is configured to provide a second fluid.
  • the present disclosure does not limit the shape of the cross section of the first dosing part 110, the first feeding part 120, the second feeding part 130, and the first discharging part 140 along the fluid flow direction.
  • the shape of the cross-section can be square, rectangular, circular, elliptical, irregular, and the like.
  • the fluid space 100 of the microfluidic substrate is formed by bonding the main body and the first cover.
  • an open channel with a certain depth can be formed on one side surface of the main body, and then the first covering member and the side surface of the main body can be combined and sealed, and the two can surround and form the fluid space.
  • the fluid space 100 of the microfluidic substrate further includes a second quantitative part 150 for quantitatively controlling the volume of the mixture of the first fluid and the second fluid.
  • the second dosing part 150 includes a third port 152 and a fourth port 154, and the third port 152 is in fluid communication with the first port 112.
  • the second quantitative part 150 is configured to quantitatively control the volume of the fluid contained therein by changing its own volume.
  • the second dosing part 150 may include a membrane pump cavity that includes an elastic membrane to control the change of the internal volume of the cavity and drive the flow of fluid.
  • Fig. 2 is a schematic cross-sectional view of a second quantitative part taken along the line A-B in Fig. 1.
  • the second quantitative part 150 includes the main body part 210 of the microfluidic substrate and the elastic membrane 230, and the second quantitative part 150 is configured to change the volume of the membrane cavity 220 by changing the deformation amount of the elastic membrane 230, thereby
  • the driving fluid enters and exits the membrane cavity 220 through the third port 152 and the fourth port 154.
  • the elastic membrane 230 When the elastic membrane 230 is pulled down, for example, by applying negative pressure to the elastic membrane 230 to pull it down, if the fourth port 154 is closed, fluid can enter the membrane cavity 220 through the third port 152. At this time, the fluid containing space enclosed by the elastic membrane 230 and the main body 210 has a second volume, so as to provide a second volume of fluid equal to the second volume.
  • the shape of the second volume is a part of a sphere (ball gap)
  • the second volume (that is, the second volume) V2 can be quantitatively calculated according to the following calculation formula (2) for the ball gap:
  • V2 ⁇ H(3D 2 +4H 2 )/24 (2)
  • D is the diameter of the cross section where the fluid contacts the main body 210
  • H is the distance between the elastic membrane 230 and the cross section along the center normal direction of the cross section where the fluid contacts the main body 210.
  • the first cover may include an elastic film 230.
  • the elastic film 230 may be a part of the first cover.
  • the fluid space 100 of the microfluidic substrate may further include a third quantitative portion 160 having a third volume V3 and configured to quantitatively control the volume of the fluid in the third quantitative portion.
  • the third volume V3 is calculated according to formula (3):
  • V3 L3*W3*D3 (3)
  • L3 is the length of the cuboid
  • W3 is the width of the cuboid
  • D3 is the height or depth of the cuboid.
  • the third quantitative portion 160 includes a fifth port 162 and a sixth port 164, and the fifth port 162 and the fourth port 154 are in fluid communication.
  • the third quantitative part 160 includes a detection site 166 to detect a mixture of the first fluid and the second fluid.
  • the detection site 166 may include antibodies or antigens, etc., so as to perform biological detection on the mixture of the first fluid and the second fluid.
  • the fluid space 100 of the microfluidic substrate further includes a filter membrane 180 located between the first feeding part 120 and the first port 112 of the first dosing part 110 for filtering the first fluid.
  • the filter membrane 180 may be a conventional blood filter membrane for filtering cells and other macromolecules in the blood.
  • the fluid space 100 of the microfluidic substrate further includes a second discharge part 170 which is in fluid communication with the sixth port 164 and is configured to contain the fluid flowing out of the third dosing part 160.
  • FIG. 3-7 are structural schematic diagrams of the main body 210, the second covering member 300, the fastener 400, the first covering member 500, and the bonding portion 600 of the microfluidic substrate according to an embodiment of the present disclosure.
  • the first feeding part 120, the second feeding part 130, the first discharging part 140 and the second discharging part 170 may penetrate through the upper and lower surfaces of the main body part 210.
  • the main body part 210 may include a channel with a certain depth formed on one side surface for forming the fluid space 100 in cooperation with the first covering member 500, that is, various functional parts (for example, the first quantitative part, the first entering part).
  • the fluid space 100 can be used for sample injection, mixing, dilution, antigen-antibody reaction, and the like.
  • the main body 210 may further include a mounting opening 310 for fixing, such as a threaded hole.
  • the main body 210 is made of plastic such as polystyrene (PS), and may be made through an injection molding process.
  • the microfluidic substrate may further include a second cover 300 on the side of the main body 210 away from the first cover 500 for sealing the fluid space. As shown in FIG.
  • the second covering member 300 may include a first sealing member 320, a first sealing member 320, a second sealing member 320, a second sealing member 320, a first sealing member 320, a second sealing member, and a first sealing member 320 respectively.
  • the second cover 300 can be used as a consumable component, which reduces the risk of sample contamination and biological exposure, and eliminates complicated cleaning steps.
  • the first sealing member 320, the second sealing member 330, the third sealing member 340, and the fourth sealing member 370 can be used to make the first feeding part 120, the second feeding part 130, and the second feeding part 120, respectively, as required.
  • the first discharge portion 140 and the second discharge portion 170 are sealed or opened to the outside air.
  • the second cover 300 may be made of silica gel, for example.
  • the microfluidic substrate may further include a fastener 400 for fastening the second cover 300 to the main body 210.
  • the fastener 400 may include a mounting member 410 for mating with the mounting opening 310 to perform a fixing function.
  • the mounting member 410 may be a screw, a buckle, or the like.
  • the fastener 400 may be made of ABS plastic.
  • FIG. 6 shows a schematic diagram of the first covering member 500, where the first covering member 500 may be a multilayer composite material.
  • the first cover 500 may include a lyophilic layer and an elastic layer.
  • the lyophilic layer provides good fluid wetting effect with fluid, while the elastic layer has good elasticity and toughness.
  • the elastic layer After being given positive and negative pressure, the elastic layer is pushed up and down and pulled up and down.
  • a single movement of the elastic layer up and down at a low frequency can play a role in pumping fluid quantitatively, and a high frequency up and down movement can play a role in mixing the fluid in the membrane cavity.
  • the elastic layer may provide a good elastic effect.
  • the lyophilic layer is made of PS, for example, and the elastic layer is made of polyethylene terephthalate (PET), for example.
  • the microfluidic substrate may further include a bonding part 600.
  • the coupling part 600 is used to couple the first cover 500 to the main body part 210.
  • the joint 600 may be a double-sided adhesive tape, on which a corresponding space is reserved for the fluid space.
  • Fig. 8 shows a schematic perspective view of the microfluidic substrate after assembly according to an embodiment of the present disclosure.
  • the microfluidic substrate includes a main body 210, a second cover 300, a fastener 400, a first cover 500, and a bonding part 600.
  • the embodiment of the present disclosure also discloses a microfluidic device, including: the above-mentioned microfluidic substrate.
  • the microfluidic device may further include a control for controlling the second cover 300 so that the first feeding part 120, the second feeding part 130, the first discharging part 140, and the second discharging part 170 are sealed or opened to the outside air.
  • an injection device for adding fluid to the first feeding part 120 and the second feeding part 130, and applying positive and negative pressure to the elastic membrane 230 of the second quantitative part 150 to deform the elastic membrane 230 so that the fluid enters and exits the second quantitative The pressure control device of the section 150 and so on.
  • an embodiment of the present disclosure also discloses a fluid driving method 900 for a microfluidic substrate, including:
  • S701 Drive the first fluid to flow through the first port to fill the first quantitative portion, and the excess first fluid flows into the first discharge portion during the filling; wherein the first quantitative portion has a first volume and is configured to provide A volume of the first volume of the first fluid.
  • the fluid driving method further includes, after driving the first fluid to flow through the first port to fill the first quantitative portion:
  • S702 Drive the second fluid to flow through the second port and enter the first dosing part, while the first fluid inside the first dosing part then flows through the first port to the second dosing part.
  • the fluid driving method further includes, after driving the second fluid to flow through the second port and into the first dosing part:
  • the second quantitative part includes a membrane pump cavity having a second volume, and the membrane pump cavity includes an elastic membrane and is configured to quantify the second volume by controlling the amount of deformation of the elastic membrane.
  • the fluid driving method further includes, after the mixture of the first fluid and the second fluid reaches a predefined volume in the second dosing part:
  • S704 Fill the third dosing part by changing the deformation of the elastic membrane to drive the mixture through the fourth port, and the fluid flowing out of the third dosing part flows into the second discharge part during the filling;
  • the third quantitative part has a third volume and is configured to quantitatively control the volume of the mixture in the third quantitative part.
  • the first fluid is driven from the first port 112 into the first dosing part 110 with a first volume under the action of an external motor, so as to excess the first fluid.
  • the first quantitative portion 110 is filled and overflows from the second port 114 to the first discharge portion 140.
  • the first discharge part 140 is open to the outside air, and the second feeding part 130 and the second dosing part 150 are sealed, so the first fluid will not flow to the channel of the second feeding part 130.
  • the sealing method of the first feeding part 120, the second feeding part 130 and the first discharging part 140 can be, for example, by means of a control device of a microfluidic device to brake the driving motor of the liquid injection piston push rod, or by means of The second cover 300 performs sealing.
  • the sealing of the second quantitative portion 150 may be, for example, by means of a control device to fix the elastic membrane 230 or maintain a certain pressure.
  • the first quantification part 110 realizes the first quantification by arranging a microchannel for bidirectional fluid flow on the microfluidic substrate and intercepting the fluid in the microchannel with a certain length.
  • the fluid in the channel with a fixed length in the middle is taken to precisely control the volume of the fluid participating in the reaction.
  • the second fluid is added to the second feeding part 130, and the elastic membrane 230 of the second dosing part 150 is deformed to drive the second fluid to flow to the first dosing part 110 through the second port 114.
  • the second feeding part 130 applies pressure to the second fluid to drive the second fluid to flow to the first dosing part 110 through the second port 114.
  • the first feeding part 120 and the first discharging part 140 are sealed. Therefore, the second fluid will drive the first fluid in the first dosing part 110 to enter the second dosing part 150.
  • the second discharge portion 170 is sealed, and the sealing method may be the same as that of the first discharge portion 140, for example.
  • the mixture of the first fluid and the second fluid will accumulate in the second dosing part 150. Since the second volume of the second quantitative portion 150 can be accurately controlled by controlling the deformation amount of the elastic membrane 230, the volume of the mixture of the first fluid and the second fluid can be correspondingly controlled to be equal to the second volume V2 of the second volume. , Since the volume of the first fluid is controlled to the first volume V1 in the previous step, the volume of the second fluid is V2-V1. Correspondingly, the ratio of the first fluid and the second fluid can be controlled.
  • the first fluid may be the fluid to be detected, and the second fluid may be a diluent.
  • the second quantitative part 150 determines the second volume contained in the elastic membrane 230 of the second quantitative part 150 by the deformation of the second quantitative part 150 based on the cross-section and the pull-down depth, thereby realizing the second quantitative.
  • the role of the second quantification is to determine the volume of the mixture of the first fluid and the second fluid.
  • the second quantification can be precisely controlled by the pulling depth or the number of pulling down of the elastic membrane 230. When the volume of the mixture of the first fluid and the second fluid is less than the maximum second volume of the second quantitative part 150, the pulling down of the elastic membrane 230 can be controlled. Depth is used to control the quantification, and vice versa, it is quantified by multiple full stroke pulldowns.
  • the second quantification part 150 is used for the second quantification, which has a simple structure and convenient operation.
  • the elastic membrane 230 of the second quantitative portion 150 can also function as a mixed fluid.
  • the first fluid and the second fluid can be diluted and mixed by the high frequency (for example, 0.5 times/second) movement of the elastic membrane 230 up and down.
  • the quantitative first fluid can be diluted by the quantitative second fluid.
  • the second discharge part 170 is opened to the outside air, the first feeding part 120, the second feeding part 130 and the first discharge part 140 are sealed, the elastic membrane 230 is pushed upward or the elastic membrane 230 is restored to the shape before deformation, Then the mixed first fluid and second fluid can be driven through the fifth port 162 into the third quantitative portion 160 having a third volume V3.
  • the third quantitative part 160 may be embedded with detection sites, such as labeled antibodies, where the antigen-antibody reaction is performed, that is, the quantitative mixture of the first fluid and the second fluid is detected.
  • the elastic membrane 230 can be pulled up and down to drive the third volume of fluid to flow back and forth in the third quantitative portion 160, so as to fully perform the mixing reaction.
  • the detection site after the reaction can be subjected to subsequent further detection, such as optical detection.
  • the third quantification unit 160 can quantitatively determine the volume of the fluid participating in the detection reaction, that is, the third quantification. By combining the third quantification part 160 with a fixed volume and a predetermined amount of detection sites, the quantification of the reactants in the detection process can be achieved. Three quantifications realize the precise quantification of the whole detection process.
  • the first quantitative part can be used to accurately control the first fluid (for example, the sample to be detected) without a flow sensor.
  • the volume of the first fluid is reduced, and the volume of the first fluid is reduced.
  • the structure is simple and the operation is convenient.
  • the second quantitative part including the elastic membrane can be used to accurately control the volume of the mixture (for example, the diluted sample) of the first fluid and the second fluid (for example, the diluent), and the structure Simple and easy to operate.
  • the embodiments of the present disclosure can realize operations such as quantitative transportation, dilution, mixing, and reaction of fluids, without complicated external sensors and fluid drive control devices, improving the integration of the system, and reducing the process complexity and cost of the system.
  • the second cover can be used as a consumable part, which reduces the risk of sample contamination and biological exposure, and saves complicated cleaning steps.

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  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
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  • General Health & Medical Sciences (AREA)
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Abstract

微流控基板及流体驱动方法和微流控装置。微流控基板包括第一定量部（110），第一定量部（110）具有第一容积并且配置成提供等于第一容积的第一体积的第一流体。

Description

微流控基板及其流体驱动方法、微流控装置
相关申请
本申请要求2019年10月17日提交的申请号为201910988391.5的中国专利申请的优先权，该专利申请的所有内容通过引用合并于此。
技术领域
本公开涉及生物检测领域，并且更特别地涉及微流控基板及其流体驱动方法、微流控装置。
背景技术
微流控装置又称为芯片实验室(Lab-on-a-chip)，是指把生物、化学和医学等领域中所涉及的样本制备、反应、分离、检测等基本操作单元集成到一块具有微米尺度微通道的芯片上，自动完成反应和分析的全过程。基于微流控装置的分析检测装置的优点是：样本用量少、分析速度快、便于制成便携式仪器以及非常适用于即时、现场分析。要实现基于微流控装置的分析检测装置的自动化和集成化，需要尽可能多地将反应、分析等的各项功能集成到装置上，而减少对装置外操作的依赖。而且，微流控装置可以设计为一次性使用产品，这样可省去复杂的清洗和废液处理等液路系统。
发明内容
在一方面，本公开实施例提供了一种微流控基板，包括：第一定量部，所述第一定量部具有第一容积并且配置成提供等于所述第一容积的第一体积的第一流体。
在一些实施例中，微流控基板还包括第一进料部、第二进料部和第一排出部，其中，所述第一定量部包括第一端口和第二端口，所述第一定量部通过所述第一端口与所述第一进料部流体连通并且通过所述第二端口分别与所述第二进料部和所述第一排出部流体连通。
在一些实施例中，所述第一进料部配置成通过所述第一端口向所述第一定量部提供大于所述第一容积的第一流体，并且所述第二进料部配置成提供第二流体。
在一些实施例中，微流控基板还包括用于定量控制第一流体与第二流体两者的混合物的体积的第二定量部，所述第二定量部包括具有第二容积的膜泵腔，所述膜泵腔包括弹性膜并且配置成通过控制所述弹性膜的形变量来对所述第二容积进行定量。
在一些实施例中，所述第二定量部包括第三端口和第四端口，所述第三端口与所述第一端口流体连通。
在一些实施例中，微流控基板还包括第三定量部，所述第三定量部具有第三容积并且配置成定量控制在所述第三定量部中的流体的体积。
在一些实施例中，所述第三定量部包括检测位点并且所述第三定量部包括第五端口与第六端口，所述第五端口与所述第四端口流体连通。
在一些实施例中，微流控基板还包括第二排出部，所述第二排出部与所述第六端口流体连通，并且配置成容纳从所述第三定量部流出的流体。
在一些实施例中，微流控基板还包括主体部与第一覆盖件，其中所述主体部与所述第一覆盖件贴合形成所述第一定量部、第一进料部、第二进料部、第一排出部、第二定量部、第三定量部以及第二排出部。
在一些实施例中，所述第一覆盖件包括亲液层与弹性层的复合结构。
在一些实施例中，微流控基板还包括位于所述主体部远离第一覆盖件的一侧的第二覆盖件，所述第二覆盖件用于对所述第一进料部、第二进料部、第一排出部以及第二排出部中的一个或多个进行密封。
在另一方面，本公开实施例还提供了一种微流控装置，包括上述的微流控基板。
在又一方面，本公开实施例还提供了一种用于微流控基板的流体驱动方法，包括：驱动第一流体流动经过第一端口而填充第一定量部，在填充期间多余的第一流体流入第一排出部；其中所述第一定量部具有第一容积并且配置成提供等于所述第一容积的第一体积的第一流体。
在一些实施例中，流体驱动方法还包括，在驱动第一流体流动经过第一端口而填充第一定量部之后：驱动第二流体流动经过第二端口而进入所述第一定量部，同时所述第一定量部内部的第一流体随之经 过所述第一端口流向第二定量部。
在一些实施例中，流体驱动方法还包括，在驱动第二流体流动经过第二端口而进入所述第一定量部之后：驱动第一流体和第二流体进入所述第二定量部，直至第一流体和第二流体的混合物在所述第二定量部中达到预定义的容量，其中所述第二定量部包括具有第二容积的膜泵腔，所述膜泵腔包括弹性膜并且配置成通过控制所述弹性膜的形变量来对所述第二容积进行定量。
在一些实施例中，流体驱动方法还包括，在第一流体和第二流体的混合物在所述第二定量部中达到预定义的容量之后：通过改变所述弹性膜的形变量来驱动所述混合物经过第四端口而填充第三定量部，在填充期间从所述第三定量部流出的流体流入第二排出部；以及对所述第三定量部中的所述混合物进行检测，其中所述第三定量部具有第三容积，并且配置成定量控制在所述第三定量部中的所述混合物的体积。
根据本公开实施例提供的微流控基板、微流控装置和流体驱动方法，在无需流量传感器的情况下，利用第一定量部，可以准确控制第一流体(例如，待检测的样本)的体积，同时减少第一流体使用体积，结构简单，操作方便。同时，在无需流量传感器的情况下，利用包括弹性膜的第二定量部，可以准确控制第一流体与第二流体(例如，稀释液)的混合物(例如，经稀释的样本)的体积，结构简单，操作方便。本公开的实施例可以实现流体的定量运输、稀释混合、反应等操作，无需复杂的外接传感器和流体驱动控制装置，提高了系统的集成度，降低了系统的工艺复杂程度和成本。另外，在本公开的实施例中，将第二覆盖件作为一个耗材部件，减少了样本污染与生物暴露的风险，省去了复杂的清洗步骤。
附图说明
为了更清楚地说明本公开实施例中的技术方案，下面将对实施例描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本公开的一些实施例。
图1为根据本公开的实施例的微流控基板的结构示意图；
图2为沿图1中的A-B线截取的第二定量部的截面示意图；
图3为根据本公开实施例的微流控基板的主体部的结构示意图；
图4为根据本公开实施例的微流控基板的第二覆盖件的结构示意图；
图5为根据本公开实施例的微流控基板的紧固件的结构示意图；
图6为根据本公开实施例的微流控基板的第一覆盖件的结构示意图；
图7为根据本公开实施例的微流控基板的结合部的结构示意图；。
图8为根据本公开实施例的微流控基板的透视示意图；以及
图9为根据本公开实施例的流体驱动方法的流程图。
具体实施方式
为使本公开实施例的目的、技术方案和优点更加清楚，下面将结合附图对本公开实施例的技术方案作进一步地详细描述。
目前在使用微流控装置的过程中，当样本注入到微流控装置的流体空间后，参与反应的体积用量无法通过简单的没有传感器的方式来精确定量。而增加流体流量传感器会增加装置作为耗材的成本和设备的复杂度，显然不利于基于微流控装置的分析检测装置的集成化、小型化与产品化。
因此，如何在不借助于流体流量传感器的情况下对待测流体进行定量，从而便于进行混合、检测等后续步骤是需要解决的技术问题。本公开的一目的是开发一种不借助于流体流量传感器而进行流体定量的微流控基板、微流控装置，以及用于微流控基板的流体驱动方法。
图1描述了一种基于本公开的实施例的微流控基板的结构示意图。微流控基板的流体空间100包括第一定量部110。第一定量部110具有第一容积并且配置成提供与该第一容积相等的第一体积的第一流体。例如，当第一定量部110为长方体时，第一体积(即第一容积)V1根据公式(1)进行计算：
V1＝L1*W1*D1      (1)，
其中L1为长方体的长度，W1为长方体的宽度，D1为长方体的高度或深度。
微流控基板的流体空间100还包括用于向流体空间中加入第一流体的第一进料部120、用于向流体空间中加入第二流体的第二进料部 130和可以容纳一定量的流体的第一排出部140。第一定量部110包括第一端口112和第二端口114。第一定量部110通过第一端口112与第一进料部120流体连通并且通过第二端口114分别与第二进料部130和第一排出部140流体连通。第一进料部120配置成通过第一端口112向第一定量部110提供体积大于第一体积V1的第一流体，并且第二进料部130配置成提供第二流体。应当理解，本公开对第一定量部110、第一进料部120、第二进料部130以及第一排出部140沿流体流动方向上的截面的形状不进行限定。该截面的形状可以为正方形、矩形、圆形、椭圆形、不规则形状等。
在一些实施例中，微流控基板的流体空间100由主体部与第一覆盖件贴合形成。换言之，可以在主体部的一侧表面上形成一定深度的开放的沟道，然后将第一覆盖件与主体部的该侧表面结合并密封，则两者可以包围形成该流体空间。
在一些实施例中，如图1所示，微流控基板的流体空间100还包括用于定量控制第一流体与第二流体的混合物的体积的第二定量部150。第二定量部150包括第三端口152和第四端口154，第三端口152与第一端口112流体连通。
第二定量部150配置成通过改变自身的容积来定量控制其中所容纳的流体的体积。例如，第二定量部150可以包括膜泵腔，该膜泵腔包括弹性膜来控制腔内容积变化以及驱动流体的流动。图2为沿图1中的A-B线截取的第二定量部的截面示意图。如图2所示，第二定量部150包括微流控基板的主体部210和弹性膜230，并且第二定量部150配置成通过改变弹性膜230的形变量来改变膜腔220的容积，从而驱动流体通过第三端口152和第四端口154进出膜腔220。当弹性膜230被下拉时，例如通过对弹性膜230施加负压强而使其下拉时，如果封闭第四端口154，则流体可以通过第三端口152进入膜腔220。此时，弹性膜230与主体部210围成的流体容纳空间具有第二容积，从而提供等于该第二容积的第二体积的流体。在一些实施例中，当第二容积的形状为球体的一部分(球缺)时，第二体积(即第二容积)V2可以依照下列球缺体积的计算公式(2)来进行定量计算：
V2＝πH(3D 2+4H 2)/24      (2)
其中D为流体与主体部210接触的截面的直径，H为沿着流体与 主体部210接触的截面的中心法线方向上弹性膜230与该截面的距离。
在一些实施例中，第一覆盖件可以包括弹性膜230。换言之，弹性膜230可以为第一覆盖件的一部分。
如图1所示，微流控基板的流体空间100还可以包括第三定量部160，第三定量部160具有第三容积V3并且配置成定量控制在第三定量部中的流体的体积。例如，当第三定量部160为长方体时，第三容积V3根据公式(3)进行计算：
V3＝L3*W3*D3       (3)
其中L3为长方体的长度，W3为长方体的宽度，D3为长方体的高度或深度。
第三定量部160包括第五端口162与第六端口164，第五端口162与第四端口154流体连通。在一些实施例中，第三定量部160包括检测位点166，从而对第一流体与第二流体两者的混合物进行检测。例如，检测位点166可以包括抗体或抗原等，从而对第一流体与第二流体两者的混合物进行生物检测。
在一些实施例中，微流控基板的流体空间100还包括滤膜180，其位于第一进料部120与第一定量部110的第一端口112之间，用于过滤第一流体。例如，当第一流体为血液时，滤膜180可以为常规滤血膜，用于过滤血液中细胞及其他大分子。
在一些实施例中，微流控基板的流体空间100还包括第二排出部170，第二排出部170与第六端口164流体连通，并且配置成容纳从第三定量部160流出的流体。
图3-7为根据本公开的实施例的微流控基板的主体部210、第二覆盖件300、紧固件400、第一覆盖件500以及结合部600的结构示意图。如图3所示，第一进料部120、第二进料部130、第一排出部140以及第二排出部170可以贯穿主体部210的上下侧表面。主体部210可以包括在一侧表面上形成的具有一定深度的沟道，以用于与第一覆盖件500配合形成流体空间100，即各个功能部(例如，第一定量部、第一进料部、第二进料部、第一排出部、第二定量部、第三定量部以及第二排出部等)以及连接不同功能部的通道等。流体空间100可以用于进样、混合、稀释、抗原抗体反应等。主体部210还可以包括用于固定的安装口310，例如螺纹孔。主体部210由诸如聚苯乙烯(PS)的塑料 制成，并且可以通过注塑工艺制成。微流控基板还可以包括在主体部210远离第一覆盖件500的一侧的第二覆盖件300，其用于对流体空间进行密封。如图4所示，第二覆盖件300可以包括分别与第一进料部120、第二进料部130、第一排出部140以及第二排出部170相配合的第一密封件320、第二密封件330、第三密封件340以及第四密封件370。在一些实施例中，第二覆盖件300可以作为一个耗材部件，减少了样本污染与生物暴露的风险，省去了复杂的清洗步骤。在一些实施例中，第一密封件320、第二密封件330、第三密封件340以及第四密封件370可以根据需要而分别使第一进料部120、第二进料部130、第一排出部140以及第二排出部170密封或开放于外界空气。第二覆盖件300可以例如由硅胶制成。微流控基板还可以包括紧固件400，紧固件400用于将第二覆盖件300紧固至主体部210。例如，紧固件400可以包括安装件410，用于与安装口310配合而发挥固定作用。例如，安装件410可以为螺钉、卡扣等。紧固件400可以由ABS塑料制成。通过第二覆盖件300以及紧固件400的使用，可以对微流控基板的流体空间进行可控地封闭和开放，便于驱动流体空间中的流体进行定向的流动。
图6示出了第一覆盖件500的示意图，其中，第一覆盖件500可以为多层复合材料。例如，第一覆盖件500可以包括亲液层与弹性层。亲液层提供与流体的良好流体润湿效果，而弹性层具有良好的弹性和韧性。在外界给予正负压后被上下推挤和抽拉，弹性层以低频率上下单次运动可以起到定量泵送流体作用，高频率上下运动可以起到混合膜腔内流体的作用。例如，在第一覆盖件500充当第二定量部150的弹性膜230的情况下，弹性层可以提供良好的弹性效果。在示例性实施例中，亲液层例如由PS制成，并且弹性层例如由聚对苯二甲酸乙二醇酯(PET)制成。微流控基板还可以包括结合部600。结合部600用于将第一覆盖件500结合至主体部210。例如，结合部600可以为双面胶，其上为流体空间预留相应的让位空间。
图8示出了根据本公开实施例的微流控基板在组装完成后的透视示意图。微流控基板包括主体部210、第二覆盖件300、紧固件400、第一覆盖件500以及结合部600。
本公开的实施例还公开了一种微流控装置，包括：上述的微流控基板。微流控装置还可以包括用于控制第二覆盖件300而使得第一进 料部120、第二进料部130、第一排出部140以及第二排出部170密封或开放于外界空气的控制装置，向第一进料部120和第二进料部130加入流体的注入装置，以及对第二定量部150的弹性膜230施加正负压强而使弹性膜230变形从而使得流体进出第二定量部150的压强控制装置等。这些装置为本领域技术人员所已知，不再赘述。
如图9所示，本公开的实施例还公开了一种用于微流控基板的流体驱动方法900，包括：
S701：驱动第一流体流动经过第一端口而填充第一定量部，在填充期间多余的第一流体流入第一排出部；其中第一定量部具有第一容积并且配置成提供等于该第一容积的第一体积的第一流体。
该流体驱动方法还包括，在驱动第一流体流动经过第一端口而填充第一定量部之后：
S702：驱动第二流体流动经过第二端口而进入第一定量部，同时第一定量部内部的第一流体随之经过第一端口流向第二定量部。
该流体驱动方法还包括，在驱动第二流体流动经过第二端口而进入第一定量部之后：
S703：驱动第一流体和第二流体进入第二定量部，直至第一流体和第二流体的混合物在第二定量部中达到预定义的容量；
其中第二定量部包括具有第二容积的膜泵腔，膜泵腔包括弹性膜并且配置成通过控制弹性膜的形变量来对第二容积进行定量。
该流体驱动方法还包括，在第一流体和第二流体的混合物在第二定量部中达到预定义的容量之后：
S704：通过改变弹性膜的形变量来驱动混合物经过第四端口而填充第三定量部，在填充期间从第三定量部流出的流体流入第二排出部；以及
S705：对第三定量部中的混合物进行检测；
其中第三定量部具有第三容积，并且配置成定量控制在第三定量部中的混合物的体积。
具体地，下面结合图1-8中示出的结构，描述根据本公开的实施例的流体驱动方法。
从第一进料部120加入的第一流体经过过滤后，在外界电机作用下将第一流体从第一端口112驱动进入具有第一容积的第一定量部110， 从而将第一流体过量充满第一定量部110并从第二端口114溢出到第一排出部140。此时第一排出部140是开放于外界空气中的，并且第二进料部130和第二定量部150是被密封的，因此第一流体不会流向第二进料部130的通道。第一进料部120、第二进料部130以及第一排出部140的密封方法可以例如为借助于微流控装置的控制装置来对注液活塞推杆的驱动电机抱闸，或者借助于第二覆盖件300进行密封。第二定量部150的密封可以例如为借助于控制装置对弹性膜230进行固定或者保持一定的压强。
第一定量部110通过在微流控基板上设置一个流体双向流动的微通道并且截取确定长度的微通道中的流体来实现第一次定量。通过将通道中第一定量部110之外的部分的流体排除在外，取中间固定长度的通道中的流体从而精确控制参与反应的流体体积。通过微流控基板的通道设计来精确定量，减少了流体的使用体积，同时精确控制了流体参与反应的量，减少流体采样量少而造成的检测失败，检测结果更加准确。
然后，在第二进料部130加入第二流体，并借助于使第二定量部150的弹性膜230发生变形而驱动第二流体通过第二端口114向第一定量部110流动。或者，通过第二加料部130对第二流体施加压强而驱动第二流体通过第二端口114向第一定量部110流动。此时，第一进料部120以及第一排出部140是密封的。因此，第二流体会驱动第一定量部110中的第一流体进入第二定量部150。此时第二排出部170被密封，密封方法例如可以与第一排出部140的密封方法相同。因此，第一流体与第二流体的混合物将在第二定量部150中累积。由于第二定量部150的第二容积可以通过控制弹性膜230的形变量而进行精确控制，则第一流体与第二流体的混合物的体积可以相应控制为等于该第二容积的第二体积V2，由于第一流体的体积在前一步骤中控制为第一体积V1，因此第二流体的体积为V2-V1。相应地，第一流体和第二流体的配比可以控制。例如，第一流体可以为待检测的流体，而第二流体可以为稀释液。
第二定量部150通过弹性膜230的变形，基于第二定量部150的截面和下拉深度确定了第二定量部150的弹性膜230一次变形所容纳的第二体积，从而实现第二次定量。第二次定量的作用是确定第一流 体和第二流体的混合物的体积。第二次定量可以通过弹性膜230的下拉深度或者下拉次数来精确控制，第一流体和第二流体的混合物的体积比第二定量部150的最大的第二容积少时通过控制弹性膜230的下拉深度来控制定量，反之则通过多次完整行程下拉来定量。利用第二定量部150进行第二次定量，结构简单，操作方便。
第二定量部150的弹性膜230还可以发挥混合流体的作用。例如，通过弹性膜230的高频率(例如0.5次/秒)上下运动可以稀释混合第一流体和第二流体，混合完成后定量的第一流体可以被定量的第二流体稀释。
最后，使第二排出部170开放于外界空气，密封第一进料部120、第二进料部130和第一排出部140，向上推弹性膜230或者使弹性膜230恢复至变形前形状，则混合的第一流体和第二流体可以被驱动通过第五端口162进入具有第三容积V3的第三定量部160。第三定量部160内可以预埋检测位点，例如标记抗体，在此处进行抗原抗体反应，即对定量的第一流体和第二流体的混合物进行检测。可选地，可以通过对弹性膜230的上下抽拉，驱动第三容积的流体在第三定量部160来回流动，从而充分地进行混合反应。可选地，还可以对发生反应后的检测位点进行后续进一步的检测，例如光学检测等。
第三定量部160可以定量确定参与检测反应的流体的体积，即第三次定量。通过结合固定容积的第三定量部160以及预定量的检测位点，可以实现检测过程的反应物的定量。三次定量实现整个检测过程的精确定量。
根据本公开实施例提供的微流控基板、微流控装置和流体驱动方法，在无需流量传感器的情况下，利用第一定量部，可以准确控制第一流体(例如，待检测的样本)的体积，同时减少第一流体使用体积，结构简单，操作方便。同时，在无需流量传感器的情况下，利用包括弹性膜的第二定量部，可以准确控制第一流体与第二流体(例如，稀释液)的混合物(例如，经稀释的样本)的体积，结构简单，操作方便。本公开的实施例可以实现流体的定量运输、稀释混合、反应等操作，无需复杂的外接传感器和流体驱动控制装置，提高了系统的集成度，降低了系统的工艺复杂程度和成本。另外，在本公开的实施例中，将第二覆盖件可以作为一个耗材部件，减少了样本污染与生物暴露的风险，省 去了复杂的清洗步骤。
如本领域技术人员将显而易见的，执行这些本公开实施例的方法的许多不同的方式是可能的。例如，可以改变步骤的顺序，或者可以并行执行一些步骤。此外，在步骤之间可以插入其他方法步骤。插入的步骤可以表示诸如本文所描述的方法的改进，或者可以与该方法无关。此外，在下一步骤开始之前，给定步骤可能尚未完全完成。
本领域的技术人员可以对本公开进行各种改动和变型而不脱离本公开的精神和范围。这样，倘若本公开的这些修改和变型属于本公开权利要求及其等同技术的范围之内，则本公开也意图包含这些改动和变型在内。

Claims (16)

  1. 一种微流控基板，包括：第一定量部，所述第一定量部具有第一容积并且配置成提供等于所述第一容积的第一体积的第一流体。
  2. 根据权利要求1所述的微流控基板，还包括第一进料部、第二进料部和第一排出部，
    其中，所述第一定量部包括第一端口和第二端口，
    所述第一定量部通过所述第一端口与所述第一进料部流体连通并且通过所述第二端口分别与所述第二进料部和所述第一排出部流体连通。
  3. 根据权利要求2所述的微流控基板，其中所述第一进料部配置成通过所述第一端口向所述第一定量部提供大于所述第一容积的第一流体，并且所述第二进料部配置成提供第二流体。
  4. 根据权利要求3所述的微流控基板，还包括用于定量控制第一流体与第二流体两者的混合物的体积的第二定量部，所述第二定量部包括具有第二容积的膜泵腔，所述膜泵腔包括弹性膜并且配置成通过控制所述弹性膜的形变量来对所述第二容积进行定量。
  5. 根据权利要求4所述的微流控基板，其中所述第二定量部包括第三端口和第四端口，所述第三端口与所述第一端口流体连通。
  6. 根据权利要求5所述的微流控基板，还包括第三定量部，所述第三定量部具有第三容积并且配置成定量控制在所述第三定量部中的流体的体积。
  7. 根据权利要求6所述的微流控基板，其中所述第三定量部包括检测位点并且所述第三定量部包括第五端口与第六端口，所述第五端口与所述第四端口流体连通。
  8. 根据权利要求7所述的微流控基板，还包括第二排出部，所述第二排出部与所述第六端口流体连通，并且配置成容纳从所述第三定量部流出的流体。
  9. 根据权利要求8所述的微流控基板，还包括主体部与第一覆盖件，其中所述主体部与所述第一覆盖件贴合形成所述第一定量部、第一进料部、第二进料部、第一排出部、第二定量部、第三定量部以及第二排出部。
  10. 根据权利要求9所述的微流控基板，其中所述第一覆盖件包括亲液层与弹性层的复合结构。
  11. 根据权利要求10所述的微流控基板，还包括位于所述主体部远离第一覆盖件的一侧的第二覆盖件，所述第二覆盖件用于对所述第一进料部、第二进料部、第一排出部以及第二排出部中的一个或多个进行密封。
  12. 一种微流控装置，包括：
    根据权利要求1-11中任一项所述的微流控基板。
  13. 一种用于微流控基板的流体驱动方法，包括：
    驱动第一流体流动经过第一端口而填充第一定量部，在填充期间多余的第一流体流入第一排出部；其中所述第一定量部具有第一容积并且配置成提供等于所述第一容积的第一体积的第一流体。
  14. 根据权利要求13所述的流体驱动方法，还包括，在驱动第一流体流动经过第一端口而填充第一定量部之后：
    驱动第二流体流动经过第二端口而进入所述第一定量部，同时所述第一定量部内部的第一流体随之经过所述第一端口流向第二定量部。
  15. 根据权利要求14所述的流体驱动方法，还包括，在驱动第二流体流动经过第二端口而进入所述第一定量部之后：
    驱动第一流体和第二流体进入所述第二定量部，直至第一流体和第二流体的混合物在所述第二定量部中达到预定义的容量，
    其中所述第二定量部包括具有第二容积的膜泵腔，所述膜泵腔包括弹性膜并且配置成通过控制所述弹性膜的形变量来对所述第二容积进行定量。
  16. 根据权利要求15所述的流体驱动方法，还包括，在第一流体和第二流体的混合物在所述第二定量部中达到预定义的容量之后：
    通过改变所述弹性膜的形变量来驱动所述混合物经过第四端口而填充第三定量部，在填充期间从所述第三定量部流出的流体流入第二排出部；以及
    对所述第三定量部中的所述混合物进行检测，
    其中所述第三定量部具有第三容积，并且配置成定量控制在所述第三定量部中的所述混合物的体积。
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