Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
  • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
  • A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
  • A01N43/76—1,3-Oxazoles; Hydrogenated 1,3-oxazoles
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
  • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
  • A01P3/00—Fungicides
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
  • A61K47/22—Heterocyclic compounds, e.g. ascorbic acid, tocopherol or pyrrolidones
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
  • A61K8/04—Dispersions; Emulsions
  • A61K8/06—Emulsions
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/34—Alcohols
  • A61K8/345—Alcohols containing more than one hydroxy group
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/35—Ketones, e.g. benzophenone
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
  • A61K8/368—Carboxylic acids; Salts or anhydrides thereof with carboxyl groups directly bound to carbon atoms of aromatic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q15/00—Anti-perspirants or body deodorants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
  • A61Q17/005—Antimicrobial preparations
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
  • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
  • A61K2800/52—Stabilizers
  • A61K2800/524—Preservatives

Definitions

• the present invention relates to the use of 1 ,4-di(benzoxazol-2’-yl)benzene as antimicrobial agent.
• preservatives To protect cosmetic or pharmaceutical compositions, household products, plastics, paper and/ or paints against mold and bacteria, most products currently on the market contain preservatives. While these preservatives protect against bacteria and fungi, studies have linked daily exposure to many of these substances to an increased risk of skin irritation, cancer and/ or endocrine problems. Thus, many manufactures are searching for alternative antimicrobial actives which allow reducing the amount of preservatives and don’t appear to pose any health risks.
• Antimicrobial active compounds furthermore play a key role for many cosmetic applications. They are, for example, added into deodorants to reduce body odor caused by bacterial growth such as by Staphylococcus or to balance the microbiome by suppressing the growth of unwanted bacteria on skin such as Staphylococcus aureus, Escherichia coli and/ or Pseudomonas aeruginosa.
• 1 ,4-di(benzoxazol-2’-yl)benzene exhibits an antimicrobial activity against a variety of microbes such as in particular Candida albicans (C. albicans), Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa) and/ or Aspergillus brasiliensis (A. brasiliensis).
• the present invention relates to the use of 1 ,4-di(benzoxazol-2’-yl)benzene as antimicrobial agent, i.e. an agent which exhibits an antimicrobial activity.
• the present invention is directed to the use of 1 ,4-di(benzoxazol-2’-yl)benzene as anti-fungal and/ or anti-bacterial agent, more in particular as an agent for killing and/ or inhibiting the growth of fungi and/ or gram-positive bacteria such as in particular C. albicans, E. coli, S. aureus, P. aeruginosa and/ or A. brasiliensis.
• the invention in another embodiment, relates to a method for killing and/ or inhibiting growth of microbial cells, in particular fungal and/ or bacterial cells, said method comprising contacting said microbial cells with 1 ,4-di(benzoxazol-2’-yl)benzene.
• the microbial cells are selected from the group consisting of fungi and/or gram-positive bacteria, more preferably from the group consisting of C. albicans, E. coli, S. aureus, P. aeruginosa and/ or A. brasiliensis as well as mixtures thereof.
• antimicrobial activity means a capability of killing and/or inhibiting the growth of microbial cells such as in particular bacteria and fungi and more in particular C. albicans, E. coli, S. aureus, P. aeruginosa and A. brasiliensis as well as mixtures thereof.
• 1.4-Di(benzoxazol-2’-yl)benzene is a colorless to light yellow, solid, which can be prepared as e.g. outlined in WO200239972 or illustrated in the example.
• 1.4-di(benzoxazol-2’-yl)benzene is preferably used in micronized form, i.e. in the form of solid particles having a mean particle size of less than 1 pm.
• said micronized form exhibits a mean particle size of at most 300 nm. Even more preferably, the micronized
• 1 ,4-di(benzoxazol-2’-yl)benzene exhibits a mean particle size selected in the range from 50 to 300 nm, more preferably in the range from 100 to 300 nm, most preferably in the range from 120 to 280 nm, such as in the range from 140 to 240 nm or in the range from 150 to 220 nm.
• mean particle size refers to the mean number-based particle size distribution D n 50 (also known as D n 0.5) as determined by laser diffraction e.g. with a Malvern Mastersizer 2000 (ISO 13320:2009).
• micronized 1 ,4-di(benzoxazol-2’-yl)benzene according to the present invention can be produced by standard micronization methods in the art as e.g. outlined in W09522959 and WO9703643 which are included herein by reference.
• the micronized 1 ,4-di(benzoxazol-2’-yl)benzene according to the present invention may be used in powder form or as a dispersion thereof.
• the micronized 1 ,4-di(benzoxazol-2’-yl)benzene according to the present invention can furthermore be in a crystalline or in a solid amorphous form. In all embodiments of the present invention it is preferred, that the micronized 1 ,4-di(benzoxazol-2’- yl)benzene is in a solid amorphous form.
• solid amorphous form refers to solid particles which are formed by fast formation/separation of a solid phase from a liquid phase in a solution or a mixture, so that the solid has no time to selectively form a crystal network and thus the obtained solid is in a predominantly disordered form (also referred to herein as ‘solid amorphous 1 ,4-di(benzoxazol-2'-yl)benzene’), which form can be unambiguously identified by XRPD analysis by not exhibiting a base line separation of the peaks at 25-28 °2Theta (Cu K-alpha Radiation) measured using a Bruker D8 Advance powder X-ray diffractometer in reflection (Bragg-Brentano) geometry with a LynxEye detector and Cu Ka radiation.
• the solid amorphous form of 1 ,4-di(benzoxazol-2’-yl)benzene is preferably prepared by a process encompassing the steps of (1) reacting terephthalic acid and 2-aminophenol to form 1 ,4-di(benzoxazol-2'- yl)benzene and (2) precipitating the thus obtained 1 ,4-di(benzoxazol-2'-yl)benzene, preferably in the presence of (ice) water to obtain solid amorphous 1 ,4-di(benzoxazol-2’-yl)benzene, which, in a consecutive step, is micronized according to standard methods in the art.
• the process can contain intermediate purification steps.
• the solid amorphous 1 ,4-di(benzoxazol- 2’-yl)benzene can be further purified by washing with a solvent, in which the 1 ,4- di(benzoxazol-2’-yl)benzene is only hardly or not soluble such as e.g. toluene and/ or an alcohol such as 1 -butanol without being limited thereto.
• the micronized 1 ,4-di(benzoxazol-2’-yl)benzene is used in form of an aqueous dispersion thereof as this eases the incorporation into the desired product form such as into cosmetic or pharmaceutical compositions.
• the amount of the 1 ,4-di(benzoxazol-2’-yl)benzene in such aqueous dispersions is preferably selected in the range from 10 to 90 wt.-%, 20 to 80 wt.-% or 30 to 70 wt-%, more preferably in the range from 25 to 60 wt.-%, most preferably in the range from 25 to 55 wt.-%, such as in the range from 25 to 50 wt.-%, in the range from 25 to 40 wt.-% or in the range from 25 to 35 wt.-%, based on the total weight of the aqueous dispersion.
• the aqueous dispersion may furthermore contain one or more additives e.g. to facilitate micronization and/ or to stabilize the aqueous dispersion e.g. against sedimentation.
• the amount (total) of the one or more additives in the aqueous dispersion is preferably selected in the range from 0.01 to 25 wt.-%, more preferably in the range from 0.1 to 20 wt.-%, most preferably in the range from 0.5 to 15 wt.-%, such as in the range from 1 to 10 wt.-%, based on the total weight of the aqueous dispersion.
• the at least one additive is preferably selected from the group of surface-active ingredients such as in particular anionic, non-ionic, amphoteric and cationic surfactants, anti-foam agents, salts, wetting agents and thickeners as well as mixtures thereof.
• alkyl polyglucosides having the generic formula C n H +n 0(C 6 HI O 05) X H, in which n is an integer selected in the range from 2 to 22 and x refers to the mean polymerization level of the glucoside moiety (mono-, di-, tri-, oligo-, and poly- glucoside).
• alkyl polyglucosides are e.g.
• the surface-active ingredient(s) are preferably used in an amount (total) selected in the range from 1 to 20 wt.-%, more preferably from 5 to 15 wt.-%, most preferably from 7 to 12.5 wt.-%, based on the total weight of the aqueous dispersion.
• anti-foam agents to be used in the aqueous dispersions are silicone oils such as in particular polydimethylsiloxanes and/ or silicon anti-foam agents such as in particular anhydrous dispersions of pyrogenic or hydrophobized silica in silicone oils.
• the anti-foam agent is simethicone. If present, such anti-foam agent(s) are preferably used in an amount (total) selected in the range from 0 to 1 wt.-%, more preferably in an amount of 0.01 to 0.2 wt.-%, based on the total weight of the aqueous dispersion.
• Suitable salts to be used in the aqueous dispersions include alkali and earth alkaline salts of phosphate, hydroxide such as e.g. disodium hydrogen phosphate and/ or sodium hydroxide. If present, the salt or mixtures thereof (total amount) are preferably used in amounts from 0.01 to 5 wt.-, more preferably from 0.1 to 4 wt.-%, most preferably from 0.5 to 2.5 wt.-%, based on the total weight of the aqueous dispersion.
• thickeners to be used in the aqueous dispersions encompass xanthan gum, gellan gum and/ or carboxymethylcellulose. Most preferably the thickener is xanthan gum or gellan gum. If present, such thickener(s) are preferably used in an amount (total) selected in the range from 0.1 to 1 wt.-%, more preferably in the range from 0.1 to 0.5 wt.-%, based on the total weight of the aqueous dispersion.
• Particularly suitable wetting agents to be used in the aqueous dispersions are (poly)propyleneglycol such as most preferably propyleneglycol. If present, the amount of such wetting agent(s) is preferably selected in the range from 0.1 to 1 wt.-%, more preferably in an amount of 0.2 to 0.6 wt.-%, based on the total weight of the aqueous dispersion.
• an aqueous dispersion consisting essentially of the micronized 1 ,4-di(benzoxazol-2’-yl)benzene, water and optionally at least one additive with all the definitions and preferences as given herein is used.
• the aqueous dispersions consist essentially of
• the term ‘consists essentially of as used according to the present invention means that the amounts of the ingredients (i) to (iv) sum up to 100 wt.-%, which is adjusted with the amount of water (iv). It is, however, not excluded that small amount of impurities or additives may be present which are, for example, introduced via the respective raw materials of the ingredients.
• the aqueous dispersions contain as additives (iii) propyleneglycol, one thickener selected from xanthan gum or gellan gum and optionally simethicone.
• 1 ,4-di(benzoxazol-2’-yl)benzene is also suitable to maintain skin homeostasis and/ or balance the skin microbiome by treating overpopulation of microorganisms on the skin such as e.g. E. coli, S. aureus and/ or P. aerugionosa.
• the amount of 1 ,4-di(benzoxazol-2’-yl)benzene employed according to the present invention is preferably selected in the range from 0.1 to 20 wt.-%, more preferably in the range from about 0.25 to 15 wt.-%, most preferably in the range from about 0.5 to 10 wt.-%, such as in the range from 0.3 to 7.5 wt.-% (always based on the active). Further suitable ranges encompass 0.25 to 10 wt.-%, 0.25 to 7.5 wt.-%, 0.25 to 5 wt.-%, 1 to 7.5 wt.-% as well as 1 to 5 wt.-%.
• the present invention relates also to the use of 1 ,4-di(benzoxazol-2’-yl)benzene for improving preservation, in particular of a product selected from the group of cosmetic and/ or pharmaceutical compositions, household products, plastics, paper and/ or paints, preferably in view of Aspergillus brasiliensis.
• the use optionally encompasses the step of appreciating the effect compared to a respective product not containing1 ,4-di(benzoxazol-2’-yl)benzene.
• the invention relates to a method of preventing microbial decay and breakdown of a product selected from the group of cosmetic and/ or pharmaceutical compositions, household products, plastics, paper and/ or paints, wherein said method comprises adding to the product 1 ,4-di(benzoxazol-2’- yl)benzene in an amount of 0.1 to 20 wt.-%, more preferably in an amount of 0.25 to 15 wt.-%, most preferably in an amount of 0.3 to 10 wt.-%, such as in an amount of 0.3 to 7.5 wt.-%, based on the total weight of the product.
• the method also encompasses the step of appreciating the result, in particular in comparison to the respective product not containing 1 ,4-di(benzoxazol-2’-yl)benzene.
• Further suitable ranges encompass 0.25 to 10 wt.-%, 0.25 to 7.5 wt.-%, 0.25 to 5 wt.- %, 1 to 7.5 wt.-% as well as 1 to 5 wt.-%.
• the invention relates to a method of preserving a product selected from the group of cosmetic and/ or pharmaceutical compositions, household products, plastics, paper and/ or paints against microbiological contamination or growth, wherein said method comprises adding to the product 1 ,4-di(benzoxazol-2’- yl)benzene in an amount of 0.1 to 20 wt.-%, more preferably in an amount of 0.25 to 15 wt.-%, most preferably in an amount of 0.3 to 10 wt.-%, such as in an amount of 0.3 to 7.5 wt.-%. Further suitable ranges encompass 0.25 to 10 wt.-%, 0.25 to 7.5 wt.-%, 0.25 to 5 wt.-%, 1 to 7.5 wt.-% as well as 1 to 5 wt.-%.
• the invention relates to a method of preventing microbial decay and breakdown of cosmetic or pharmaceutical compositions furthermore comprising water and at least one further agent selected from the group consisting of surfactants, emulsifiers, thickeners, and oils as such compositions are particular sensitive to microbial growth.
• the present invention furthermore relates to the use of 1 ,4-di(benzoxazol-2’- yl)benzene as deodorant compound as 1 ,4-di(benzoxazol-2’-yl)benzene has an antimicrobial action against the bacteria which are responsible for the decomposition of sweat and thus for the formation of the odor.
• 1 ,4-di(benzoxazol-2’-yl)benzene can also be used in admixture with traditional preservatives to improve the preservative activity thereof.
• a particular suitable mixture is the combination of 1 ,4-di(benzoxazol-2’-yl)benzene with at least one compound selected from the group of hydroxyacetophenone, an alkanediol and benzoic acid or a salt thereof, as 1,4-di(benzoxazol-2’-yl)benzene synergistically enhances the antimicrobial activity of said compounds.
• Said mixtures are still novel.
• the present invention also relates to a mixture comprising 1 ,4- di(benzoxazol-2’-yl)benzene and at least one compound selected from the group of hydroxyacetophenone, an alkanediol and benzoic acid or a salt thereof.
• said mixture is used as an antimicrobial agent mixture, which can be added to any product as defined herein to improve its resistance against microorganisms. It is well understood, that the mixture can be added as a pre-mix to the respective product, alternatively, however, the 1 ,4-di(benzoxazol-2’-yl)benzene and at least one compound selected from the group of hydroxyacetophenone, an alkanediol and benzoic acid or a salt thereof can also be added separately to the respective product.
• said mixture or a product comprising said mixture does not comprise a hyperbranched copolymer, such as in particular a hyperbranched of the monomers dodecenyl succinic acid anhydride, diisopropanol amine, bis- dimethylaminopropyl amine having terminal groups of formula
• the weight ratio of 1 ,4-di(benzoxazol-2’-yl)benzene to the at least one compound selected from the group of hydroxyacetophenone, an alkanediol and benzoic acid or a salt thereof is preferably selected in the range of between 40:1 to 1 :40, more preferred between 30:1 to 1 :30, even more preferred between 20:1 and 1 :20 such as in the range of 15:1 to 1 :15 or 15:1 to 1 :10.
• the weight-ratio is selected in the range of between 30:1 to 1 :2, such as between 25:1 to 1 :1.
• the weight-ratio is selected in the range of between 15 to 1 to 1 to 5, such as between 10:1 to 1 to 1 :3.
• the weight-ratio is selected in the range of between 30:1 to 1 :1 , more preferably between 25:1 to 1 :1.
• the weight-ratio of 1,4-di(benzoxazol-2’-yl)benzene to the at least one compound selected from the group of hydroxyacetophenone, an alkanediol and benzoic acid or a salt thereof is > 0.5, more preferably > 0.75, most preferably > 1 .
• hydroxyacetophenone refers to o-, m- or p-hydroxyacetophenone. Particularly preferred in all embodiments of the present invention is p-hydroxyacetophenone [CAS 99-93-4] which is also called 1-(4-hydroxyphenyl)- ethanone and which is e.g. commercially available at Symrise as SymSave® H.
• the amount of hydroxyacetophenone used is selected in the range of 0.001 to 5 wt.-%, more preferably in the range of 0.01 to 4 wt-%, most preferably in the range of 0.1 to 3 wt-%, based on the total weight of the product. Further preferred ranges are 0.005 to 4.5 wt-%, 0.05 to 4 wt-%, and 0.25 to 3 wt-%, based on the total weight of the product.
• Alkane diols such as in particular straight chain and branched Ci-i 0 alkane diols are well known preservation boosters for cosmetic applications.
• Particularly suitable alkane diols according to the present invention encompass straight chain 1 ,2-, I rrespectively 1 ,4-alkane diols which comprise from about 2 to about 10 carbon atoms, e.g., from about 3 to about 8 carbon atoms, or from about 3 to about 7 carbon atoms, such as in particular 1 ,2-propane diol, 1 ,3-propanediol, 1 ,4-butanediol, 1 ,2-pentanediol, 1 ,2-hexanediol, 1 ,2-octanediol and 1,2-decanediol.
• alkane diol in all embodiments of the present invention is 1 ,3-propandiol [CAS 504-63-2] which is e.g. commercially available under the tradename ZemeaTM Propandiol at DuPont Tate & Lyle Bioproducts.
• the amount of the alkane diol used is preferably selected in the range of 0.01 to 6 wt.-%, more preferably in the range of 0.1 to 5 wt-%, most preferably in the range of 0.5 to 4 wt-%, based on the total weight of the product. Further preferred ranges are 0.5 to 3.5 wt-% and 0.5 to 3 wt.-%, based on the total weight of the product.
• Benzoic acid as well as salts thereof are well known preservatives commonly used in foods, pharmaceuticals and cosmetics.
• Preferred salts are the potassium or sodium salts, most preferably the sodium salt.
• benzoic acid [CAS 65-85-0] and/ or sodium benzoate [CAS 532-32-1], most preferably of sodium benzoate which is e.g. commercially available at Emerald Kalama Chemicals under the trade name Purox® S Grains.
• the amount of the benzoic acid or a salt thereof used is selected in the range of 0.001 to 6 wt.-%, more preferably in the range of 0.01 to 4 wt-%, most preferably in the range of 0.1 to 3 wt-%, based on the total weight of the product. Further preferred ranges encompass 0.1 to 1 wt-% and 0.1 to 0.8 wt.-%, based on the total weight of the product.
• the use according to the invention of 1 ,4-di(benzoxazol-2’-yl)benzene, optionally in combination with at least one compound selected from the group of hydroxyacetophenone, an alkane diol and benzoic acid or a salt thereof can take place both in the cosmetic sense and in the pharmaceutical sense.
• the use is however preferably cosmetic (non- therapeutic).
• compositions according to the present invention are in particular compositions intended to be topically applied to mammalian keratinous tissue such as in particular to human skin or the human scalp and hair.
• cosmetic composition refers to cosmetic compositions as defined under the heading "Kosmetika” in Rompp Lexikon Chemie, 10th edition 1997, Georg Thieme Verlag Stuttgart, New York as well as to cosmetic compositions as disclosed in A. Domsch, "Cosmetic Compositions", Verlag fiir chemische Industrie (ed. H. Ziolkowsky), 4 th edition, 1992.
• Suitable surfactants, emulsifiers, thickeners, and oils for the purpose of the present inventions are all surfactants, emulsifiers, thickeners, and oils commonly used in cosmetic applications and which are e.g. listed in the International Cosmetic Ingredient Dictionary & Handbook by Personal Care Product Council (http://www.personalcarecouncil.org/), accessible by the online INFO BASE (http://online.personalcarecouncil.org/jsp/Home.jsp), without being limited thereto.
• compositions according to the present invention are generally prepared by admixing 1 ,4-di(benzoxazol-2’-yl)benzene, preferably in the form of an aqueous dispersion as defined herein in an amount (based on 1 ,4-di(benzoxazol-2’- yl)benzene ) selected in the range 0.1 to 20 wt.-%, more preferably in the range from about 0.25 to 15 wt.-%, most preferably in the range from about 0.3 to 10 wt.-% such as in the range from 0.3 to 7.5 wt.-%, based on the total weight of the composition with a suitable carrier. Further suitable ranges encompass 0.25 to 10 wt.-%, 0.25 to 7.5 wt.-%, 0.25 to 5 wt.-%, 1 to 7.5 wt.-% as well as 1 to 5 wt.-%.
• the cosmetic or pharmaceutical compositions according to the present invention preferably further comprise a physiologically acceptable medium, that is to say a medium compatible with keratinous substances, such as the skin, mucosa, and keratinous fibres.
• a physiologically acceptable medium is a cosmetically or pharmaceutically acceptable carrier.
• cosmetically or pharmaceutically acceptable carrier refers to all carriers and/or excipients and/ or diluents conventionally used in cosmetic compositions or pharmaceutical compositions.
• the cosmetic or pharmaceutical compositions according to the invention are in the form of a suspension or dispersion in solvents or fatty substances, or alternatively in the form of an emulsion or micro emulsion (in particular of O/W- or W/O-type), PIT-emulsion, nano emulsion, multiple emulsion (e. g. O/W/O- or W/O/W- type), pickering emulsion, hydrogel, lipogel, one- or multiphase solution or vesicular dispersion.
• an emulsion or micro emulsion in particular of O/W- or W/O-type
• PIT-emulsion nano emulsion
• multiple emulsion e. g. O/W/O- or W/O/W- type
• pickering emulsion hydrogel, lipogel, one- or multiphase solution or vesicular dispersion.
• the cosmetic or pharmaceutical compositions in accordance with the invention can be in the form of a liquid, lotion, a thickened lotion, a gel, a cream, a milk, an ointment or a paste.
• the cosmetic or pharmaceutical compositions according to the invention generally have a pH in the range from 3-10, preferably in the range from pH of 3-8, most preferred in the range from pH 3.5-7.5.
• the pH is adjusted by methods known to a person skilled in the art, e.g. by using an acid such as a hydroxy acid including glycolic acid, lactic acid, malic acid, citric acid and tartaric acid or a base such as e.g. sodium or potassium hydroxide or ammonium hydroxide as well as mixtures thereof.
• the acid or the base if present, is used in an amount of at least 0.0001 wt.-%, such as e.g. in an amount of 0.01-1 wt.-%, in particular in an amount of 0.01 to 0.5 wt.-%.
• the cosmetic compositions according to the present invention are in particular skin care preparations, functional preparations and/ or hair care preparations such as most in particularly skin or hair care preparations.
• Examples of skin care preparations are, in particular, light protective preparations (sun care preparations), anti-ageing preparations, preparations for the treatment of photo-ageing, body oils, body lotions, body gels, treatment creams, skin protection ointments, moisturizing preparations such as moisturizing gels or moisturizing sprays, face and/or body moisturizers, as well as skin lightening preparations.
• light protective preparations unsun care preparations
• anti-ageing preparations preparations for the treatment of photo-ageing
• body oils body lotions, body gels, treatment creams, skin protection ointments
• moisturizing preparations such as moisturizing gels or moisturizing sprays
• face and/or body moisturizers as well as skin lightening preparations.
• Examples of functional preparations are cosmetic compositions containing active ingredients such as hormone preparations, vitamin preparations, vegetable extract preparations, anti-ageing preparations, and/or antimicrobial (antibacterial or antifungal) preparations without being limited thereto.
• hair care preparations which are suitable according to the invention and which may be mentioned are shampoos, hair conditioners (also referred to as hair rinses), hairdressing compositions, hair tonics, hair regenerating compositions, hair lotions, water wave lotions, hair sprays, hair creams, hair gels, hair oils, hair pomades or hair brilliantines. Accordingly, these are always preparations which are applied to the hair and the scalp for a shorter or longer time depending on the actual purpose for which they are used.
• the skin care preparation is a sun care preparation.
• said sun care preparation comprises at least one UV filter.
• the UV filter may be a liquid or solid UV filter.
• the UV filter may be a UV-A or UV-B filters. Suitable liquid UV-filter absorb light in the UVB (280 - 315 nm) and/ or UVA (315 - 400 nm) range and are liquid at ambient temperature (i.e. 25°C).
• Such liquid UV-filter are well known to a person in the art and encompass in particular cinnamates such as e.g. octyl methoxycinnamate (PARSOL® MCX) and isoamyl methoxycinnamate (Neo Heliopan® E 1000), salicylates such as e.g.
• ethylhexyl salicylate also known as ethylhexyl salicylate, 2 ethylhexyl 2-hydroxybenzoate, PARSOL® EHS
• acrylates such as e.g. octocrylene (2 ethylhexyl 2-cyano-3,3-diphenylacrylate, PARSOL® 340) and ethyl 2-cyano-3,3 diphenylacrylate
• esters of benzalmalonic acid such as in particular dialkyl benzalmalonates such as e.g.
• di (2-ethylhexyl) 4- methoxybenz-'ahmalonate and polysilicone 15 PARSOL® SLX
• dialkylester of naphthalates such as e.g. diethylhexyl 2,6-naphthalate (Corapan® TQ)
• syringylidene maknna-'tes such as e.g. diethylhexyl syringylidene malonate (Oxynex® ST liquid) as well as benzotriazolyl dodecyl p-cresol (Tinoguard® TL) as well as benzophenone-3 and drometrizole trisiloxane.
• Particular advantageous liquid UV-filter are octyl methoxycinnamate, homosalate, ethylhexyl salicylate, octocrylene, diethylhexyl 2,6-naphthalate, diethylhexyl syringylidene malonate, benzotriazolyl dodecyl p-cresol, benzo-phenone-3, drometrizole trisiloxane, Polysilicone-15 as well as mixtures thereof.
• Suitable solid UV-filter absorb light in the UVB and/ or UVA range and are solid at ambient temperature (i.e. 25°C). They are particularly solid organic UV filters. Particularly suited solid UV-filters are of the group consisting of bis-ethyl- hexyhoxyphenol methoxyphenyl triazine, butyl methoxydibenzoyl methane, methylene bis-benzotriazolyl tetramethylbutylphenol, diethylamino hydroxybenzoyl hexyl benzoate, ethylhexyl triazone, diethylhexyl butamido triazone, and 4-methyhbenzylhdene camphor.
• the amount of an individual organic UV filter is preferably in the range of 0.1 to about 10 % by weight, preferable in the range of 0.5 to 7.55 % by weight, most preferably in the range of 1 to 5 % by weight, based on the total weight of the sun care preparation.
• the total amount of organic UV filter (s) depends strongly on the targeted UV protection of said composition and is typically in the range of between 1 to 50% by weight, preferably between 5 to 40% by weight, based on the total weight of said sun care preparation.
• a sun care preparation with an SPF 50 comprises preferably a total amount of organic UV filter (s) of between 15 to 50% by weight, more preferably between 20 and 40 % by weight, based on the total weight of said sun care preparation.
• the cosmetic compositions according to the present invention are emulsions and/ or gels. Even more preferably, the cosmetic compositions are emulsions which contain an oily phase and an aqueous phase such as in particular O/W, W/O, Si/W, W/Si, O/W/O, W/O/W multiple or a pickering emulsions.
• the amount of the oily phase (i.e. the phase containing all oils and fats including the polar oils) present in such emulsions is preferably at least 10 wt.-%, such as in the range from 10 to 60 wt.-%, preferably in the range from 15 to 50 wt.-%, most preferably in the range from 15 to 40 wt.-%, based on the total weight of the topical composition.
• the amount of the aqueous phase present in such emulsions is preferably at least 20 wt.-%, such as in the range from 20 to 90 wt.-%, preferably in the range from 30 to
• 80 wt.-% most preferably in the range from 30 to 70 wt.-%, based on the total weight of the topical composition.
• the O/W emulsions according to the present invention advantageously comprise (i) 1 ,4-di(benzoxazol-2’-yl)benzene in an amount selected in the range from 0.1 to
• wt. % 20 wt. %, more preferably in the range from about 0.25 to 15 wt. %, most preferably in the range from about 0.3 to 10 wt.-% such as in the range from 0.3 to 7.5 wt. %, based on the total weight of the composition, (ii) water and (iii) at least one O/W- or Si/W-emulsifier selected from the list of glycerylstearatcitrate, glycerylstearate (self- emulsifying), stearic acid, salts of stearic acid, polyglyceryl-3- methylglycosedistearate, ceteareth-20, steareth-2, steareth-12, PEG-40 stearate, phosphate esters and the salts thereof such as cetyl phosphate (Amphisol® A), diethanolamine cetyl phosphate (Amphisol® DEA), potassium cetyl phosphate (Amphisol® K),
• one or more synthetic polymers may be used as an emulsifier such as for example, PVP eicosene copolymer, acrylates/C 10-3o alkyl acrylate crosspolymer, acrylates/steareth-20 methacrylate copolymer, PEG-22/dodecyl glycol copolymer, PEG-45/dodecyl glycol copolymer, and mixtures thereof.
• PVP eicosene copolymer acrylates/C 10-3o alkyl acrylate crosspolymer
• acrylates/steareth-20 methacrylate copolymer acrylates/steareth-20 methacrylate copolymer
• PEG-22/dodecyl glycol copolymer PEG-45/dodecyl glycol copolymer, and mixtures thereof.
• the O/W-emulsifier is selected from the group of cetyl phosphates such as in particular potassium cetyl phosphate (commercially available as Amphisol® K), glyceryl stearate (and) PEG- 100 stearate (commercially available as Arlacel® 165), Polyacrylamide, Sodium Polyacrylate, Sodium Polyacryloyldimethyl Taurate, Decyl Glucoside, Ceteral Glucoside, Caprylyl/Capryl Glucoside, Sorbitan Olivate, Polygelyceryl-4 Olivate, Polyglyceryl-3 Methylglucose Distearate, Polysorbate 20, Polysorbate 60 and/ or polyalkylenglycolether such as in particular laureth-35 (lauryl alcohol with 35 EO units; commercially available as Brij® 35).
• cetyl phosphates such as in particular potassium cetyl phosphate (commercially available as Amphisol® K), glyceryl stearate (and
• the at least one O/W emulsifier is preferably used in an amount of about 0.001 to 10 wt.-%, more preferably in an amount of 0.1 to 7 wt.-% with respect to the total weigh of the composition.
• the cosmetic composition in the form of a O/W emulsion contains advantageously at least one co-emulsifier selected from the list of alkyl alcohols such as Cetyl Alcohol (Lorol C16, Lanette 16) Cetearyl Alcohol (Lanette® O), Stearyl Alcohol (Lanette® 18), Behenyl Alcohol (Lanette® 22), Glyceryl Monostearate, Glyceryl Myristate (Estol® 3650), Hydrogenated Coco- Glycerides (Lipocire Na10) without being limited to this and mixtures thereof.
• alkyl alcohols such as Cetyl Alcohol (Lorol C16, Lanette 16) Cetearyl Alcohol (Lanette® O), Stearyl Alcohol (Lanette® 18), Behenyl
• the W/O emulsions according to the present invention advantageously comprise (i) 1 ,4-di(benzoxazol-2’-yl)benzene in an amount selected in the range from 0.1 to 20 wt. %, more preferably in the range from about 0.25 to 15 wt. %, most preferably in the range from about 0.3 to 10 wt.-% such as in the range from 0.3 to 7.5 wt.
• % based on the total weight of the composition, (ii) water and (iii) at least one W/O- or W/Si-emulsifier selected from the list of polyglyceryl-2-dipolyhydroxystearat, PEG-30 dipolyhydroxystearat, cetyl dimethicone copolyol, polyglyceryl-3 diisostearate polyglycerol esters of oleic/isostearic acid, polyglyceryl-6 hexaricinolate, polyglyceryl- 4-oleate, polygylceryl-4 oleate/PEG-8 propylene glycol cocoate, magnesium stearate, sodium stearate, potassium laurate, potassium ricinoleate, sodium cocoate, sodium tallowate, potassium castorate, sodium oleate, and mixtures thereof.
• W/O- or W/Si-emulsifier selected from the list of polyglyceryl-2-dipolyhydroxystearat, PEG-30 dip
• W/Si-emulsifiers are Lauryl Polyglyceryl-3 Polydimethylsiloxyethyl Dimethicone and/or PEG-9 Polydimethylsiloxyethyl Dimethicone and/or Cetyl PEG/PPG-10/1 Dimethicone and/or PEG-12 Dimethicone Crosspolymer and/or PEG/PPG-18/18 Dimethicone.
• the at least one W/O emulsifier is preferably used in an amount of about 0.001 to 10 wt.-%, more preferably in an amount of 0.2 to 7 wt.-% with respect to the total weigh of the composition.
• the gel preparations according to the present invention advantageously comprise (i) 1 ,4-di(benzoxazol-2’-yl)benzene in an amount selected in the range from 0.1 to 20 wt. %, more preferably in the range from about 0.25 to 15 wt. %, most preferably in the range from about 0.3 to 10 wt.-% such as in the range from 0.3 to 7.5 wt. %, based on the total weight of the composition, (ii) water and (iii) at least one water soluble thickener.
• water-soluble thickeners are well known to a person skilled in the art and are e.g. listed in the "Handbook of Water soluble gums and resins" by Robert L. Davidson (Me Graw Hill Book Company (1980)).
• Particularly suitable water soluble thickeners are selected from the group consisting of polyacrylic acids (e.g. commercially available under the tradename Carbomer or Carbopol ® ), homopolymers of 2-Acrylamido-2-methylpropansulfonic acid (e.g. commercially available as Rheothik ® 11-80), acrylate copolymers (e.g. commercially available under the tradename Pemulen ® or Aculyn ® 33), branched
• Poly(methacryloyloxyethyltrimethylammoniumchlorid) (INCI-name Polyquaternium- 37), non-modified guar gums (e.g. commercially available under the tradename Jaguar), starch or derivatives thereof and/ or hydroxyalkylcelluloses.
• the water-soluble thickener is used in an amount of about 0.001 to 10 wt.-%, more preferably in an amount of 0.1 wt.-% to 7 wt.-%, based on the total weigh of the composition.
• a mixture of 702 g polyphosphoric acid and 4.28 ml methanesulfonic acid was heated to 90°C. 65 g terephthalic acid and 107 g 2-aminophenol were added. The mixture was stirred under inert atmosphere at 180°C for 8 hours and then transferred to ice water. The precipitated product was filtered and washed with water and acetic acid. The precipitate was dispersed in water and the pH adjusted to 8.0 with sodium hydroxide, filtered and washed with water.
• DBO aq. a 30% aqueous dispersion of micronized 1 ,4-di(benzoxazol-2'-yl)benzene (in the following referred to as DBO aq.) was obtained which was used in the antimicrobial tests and formulations as outlined below.
• Antimicrobial activity was assessed in analogy to the regulatory challenge test method (NF EN ISO11930).
• mixtures of the respective active(s) with physiological serum with 0.85 wt.-% NaCI in the concentrations as outlined below are prepared under sterile conditions.
• Controls were also prepared under sterile conditions.
• the control as well as the mixtures were then deposed in 96-deep well plates (1.6 ml/well).
• the wells were contaminated with the respective bacterial or the fungal strains at 2.5*10 5 to 5.6*10 5 cfu/ml for the bacteria and 1 *10 4 to 2.5*10 4 cfu/ml for the fungi to obtain the initial contamination as outlined below.
• each well was thoroughly mixed to ensure a homogeneous distribution of microbes. Then each plate was incubated at 22°C for 24h. The counting of the (remaining) population is carried out 24h after contamination. Then, the average log reduction is calculated [-Iog10 ⁇ (value inoculum)/ (value sample) ⁇ ] which is represented in tables 1-5.
• 1 ,4-di(benzoxazol-2’-yl)benzene has an antimicrobial effect over a broad range of microbes and can thus be used as preservation booster. Since it is known that typically the water phase of a product is most susceptible to microbial growth, 1 ,4-di(benzoxazol-2’-yl)benzene is thus particularly suitable to protect products comprising a water phase such as e.g. cosmetic compositions in the form of an O/W or W/O emulsion against microbial decay and breakdown and is thus suitable for improving its preservation.
• Table 3 Synergistic effect with p-Hydroxyacetophenone (HAP)
• DBO synergistically enhances the antimicrobial activity of p-Hydroxyacetophenone.

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