WO2021042319A1 - 血液细胞分析仪的分析方法和血液细胞分析仪 - Google Patents

血液细胞分析仪的分析方法和血液细胞分析仪 Download PDF

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Publication number
WO2021042319A1
WO2021042319A1 PCT/CN2019/104497 CN2019104497W WO2021042319A1 WO 2021042319 A1 WO2021042319 A1 WO 2021042319A1 CN 2019104497 W CN2019104497 W CN 2019104497W WO 2021042319 A1 WO2021042319 A1 WO 2021042319A1
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WIPO (PCT)
Prior art keywords
preheating
pool
blood cell
cell analyzer
diluent
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PCT/CN2019/104497
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English (en)
French (fr)
Inventor
程蛟
滕锦
Original Assignee
深圳迈瑞生物医疗电子股份有限公司
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Application filed by 深圳迈瑞生物医疗电子股份有限公司 filed Critical 深圳迈瑞生物医疗电子股份有限公司
Priority to CN201980098902.4A priority Critical patent/CN114174833A/zh
Priority to PCT/CN2019/104497 priority patent/WO2021042319A1/zh
Publication of WO2021042319A1 publication Critical patent/WO2021042319A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Definitions

  • This application relates to the technical field of blood cell analyzers, and more specifically to an analysis method of a blood cell analyzer and a blood cell analyzer.
  • the diluent During the blood sample analysis process of the blood cell analyzer, if four or five classifications of the impedance method are required, it is usually necessary to heat the diluent to reach the reaction temperature required for the four or five classifications. After the diluent changes from low temperature to high temperature, the gas dissolved in the diluent will be precipitated due to the temperature difference, and it will exist in the preheating tank in the form of bubbles, and the amount of gas will be precipitated with the prolongation of the sleep time of the blood cell analyzer And increase.
  • This application is made in order to solve the above-mentioned problems.
  • This application provides an analysis solution for a hematology analyzer, which exhausts bubbles in the preheating pool after the hematology analyzer exits dormancy, so that the hematology analyzer performs white blood cell counting and classification based on the dilution of the bubbles that have been removed. Therefore, the white blood cell count and classification results will not be affected due to the air bubble problem, which improves the reliability of the blood analysis results.
  • an analysis method of a blood cell analyzer comprising: after the blood cell analyzer exits dormancy, expelling air bubbles in the preheating pool of the blood cell analyzer; And sending the diluent in the preheating tank from which the air bubbles are discharged to the counting tank of the blood cell analyzer for white blood cell counting and classification.
  • the process of discharging bubbles in the preheating pool includes: sucking out part of the diluent in the preheating pool so that the bubbles stay on the top of the preheating pool where the preheating pool is emptied; And adding new diluent to the preheating pool to refill the preheating pool to discharge the gas on the top of the preheating pool.
  • the process of discharging air bubbles in the preheating pool further includes: the syringe of the blood cell analyzer is in communication with the preheating pool, and the syringe is used to remove the bubbles from the preheating pool.
  • the syringe discharges the diluent sucked from the preheating tank through the first pipeline; the syringe is connected to the diluent barrel, and The syringe sucks out new diluent from the diluent barrel for replenishing new diluent into the preheating pool; and the syringe is in communication with the preheating pool, and the syringe will The new diluent sucked by the diluent barrel is added to the preheating tank to refill the preheating tank.
  • the analysis method further includes: lowering the temperature of the preheating pool after the blood cell analyzer enters dormancy again.
  • the analysis method further includes: after the blood cell analyzer enters dormancy again, timing the time when the blood cell analyzer enters dormancy; and enters the dormancy based on the blood cell analyzer
  • the length of the dormant time is used to adjust the temperature of the preheating pool to reduce the amount of gas precipitated by the diluent in the preheating pool during the dormant period of the blood cell analyzer.
  • the adjusting the temperature of the preheating pool based on the length of time the blood cell analyzer enters dormancy includes: as the time during which the blood cell analyzer enters dormancy becomes longer And gradually lower the temperature of the preheating pool.
  • the adjusting the temperature of the preheating pool based on the length of time the blood cell analyzer enters dormancy includes: when the blood cell analyzer just enters dormancy, adjusting the temperature of the preheating pool The temperature of the preheating pool is set to a first temperature; when the time period during which the blood cell analyzer enters dormancy reaches the first time period, the temperature of the preheating pool is set to a second temperature, and the second temperature Is lower than the first temperature; and when the time period during which the blood cell analyzer enters sleep reaches a second time period, the temperature of the preheating pool is set to a third temperature, and the second time period is greater than all In the first time period, the third temperature is lower than the second temperature.
  • the analysis method further includes: after the blood cell analyzer enters sleep, timing the time when the blood cell analyzer enters sleep, and based on the timing at predetermined time intervals Just expel the air bubbles in the preheating tank.
  • the analysis method further includes: expelling air bubbles in the preheating pool of the blood cell analyzer each time before the blood cell analyzer performs white blood cell counting and classification.
  • a blood cell analyzer in another aspect of the present application, includes: a sampling component for sending the collected blood sample into a counting cell; and a preheating cell for checking before entering the counting cell
  • the hemolytic agent supply component is used to supply the hemolytic agent to the counting cell; the counting cell is used to hemolyze the diluted blood, and perform white blood cell counting and classification; and degassing
  • the device is used to discharge the bubbles in the preheating pool after the counting pool exits the dormancy, and the diluent of the bubbles is discharged to count and classify white blood cells.
  • the bubble ejection device is also used to deliver the diluent to the preheating pool and the counting pool.
  • the bubble ejection device is a syringe
  • the syringe refills by sucking out part of the diluent in the preheating tank and refilling the preheating tank with new diluent.
  • the preheating tank realizes the discharge of air bubbles in the preheating tank.
  • the blood cell analyzer further includes a first valve, a second valve, and a diluent barrel for providing diluent, wherein: the syringe is based on the first valve and the second valve The connection between the second valve and the preheating tank and the connection between the second valve and the preheating tank are used to suck out part of the diluent from the preheating tank so that the top of the preheating tank is emptied; the syringe is based on the first valve and the preheating tank.
  • the first pipeline is connected to discharge the diluent sucked from the preheating tank through the first pipeline; the syringe is based on the communication between the first valve and the second valve and the The second valve communicates with the diluent barrel to suck out new diluent from the diluent barrel for refilling the preheating pool with new diluent; the syringe is based on the first valve and the The communication of the second valve and the communication between the second valve and the preheating tank make the new diluent sucked from the diluent barrel fill the preheating tank to refill the preheating tank .
  • the blood cell analyzer further includes: a preheating pool temperature adjustment device for reducing the temperature of the preheating pool after the counting pool enters dormancy again.
  • the blood cell analyzer further includes: a sleep time timing device for timing the time when the counting cell enters sleep after the counting cell enters sleep again; and a warm-up cell
  • the temperature adjusting device is used for adjusting the temperature of the preheating tank based on the length of time the counting cell enters the dormancy, so as to reduce the amount of gas precipitated by the diluent in the preheating tank during the dormancy of the counting cell.
  • the adjustment of the temperature of the preheating pool by the preheating pool temperature adjusting device includes: gradually lowering the preheating pool as the time for the counting pool to enter sleep becomes longer temperature.
  • the adjustment of the temperature of the preheating pool by the preheating pool temperature adjusting device includes: setting the temperature of the preheating pool to the first when the counting pool just enters sleep. A temperature; set the temperature of the preheating pool to a second temperature when the time period during which the counting cell enters dormancy reaches the first time period, and the second temperature is lower than the first temperature; and When the time period for the counting pool to enter sleep reaches the second time period, the temperature of the preheating pool is set to a third temperature, the second time period is greater than the first time period, and the third temperature is lower than The second temperature.
  • the blood cell analyzer further includes: a sleep time timing device for timing the time when the counting cell enters sleep after the counting cell enters sleep; and the exhaust The bubble device is also used to discharge bubbles in the preheating pool at predetermined time intervals based on the timing of the sleep time timing device after the counting pool enters sleep.
  • the bubble ejection device is further used to exhaust the bubbles in the preheating pool before each time the counting and sorting of white blood cells is performed in the counting pool.
  • the air bubbles in the preheating pool are discharged after the blood cell analyzer exits dormancy, so that the blood cell analyzer performs white blood cell count and classification based on the existing
  • the bubble-removing diluent does not affect the white blood cell count and classification results due to bubble problems, and improves the reliability of blood analysis results.
  • Fig. 1 shows a schematic flowchart of an analysis method of a blood cell analyzer according to an embodiment of the present application
  • FIGS. 2A to 2E show schematic diagrams of the discharge process of air bubbles in the preheating pool in the analysis method of the blood cell analyzer according to the embodiment of the present application;
  • Fig. 3 shows a schematic flowchart of an analysis method of a blood cell analyzer according to another embodiment of the present application
  • Fig. 4 shows a schematic flow chart of an analysis method of a blood cell analyzer according to still another embodiment of the present application
  • FIG. 5 shows a schematic flowchart of an analysis method of a blood cell analyzer according to another embodiment of the present application
  • Fig. 6 shows a schematic block diagram of a blood cell analyzer according to an embodiment of the present application
  • Fig. 7 shows a schematic block diagram of a blood cell analyzer according to another embodiment of the present application.
  • Fig. 8 shows a schematic block diagram of a blood cell analyzer according to still another embodiment of the present application.
  • Fig. 9 shows a schematic block diagram of a blood cell analyzer according to another embodiment of the present application.
  • the blood cell analyzer in the embodiment of the present application includes a preheating pool and a counting pool.
  • the preheating pool heats the diluent to a certain temperature, and then the heated diluent is sent to the counting pool to dilute the blood sample.
  • Counting and classifying white blood cells based on the blood diluted with the heated diluent can obtain better counting and classifying results.
  • heating the dilution solution is the key to realize the four and five classifications of animal white blood cells.
  • the blood cell analyzer in this application is preferably an animal blood cell analyzer.
  • the analysis method 100 of the blood cell analyzer may include the following steps:
  • step S110 after the blood cell analyzer exits dormancy, the air bubbles in the preheating pool of the blood cell analyzer are discharged.
  • step S120 the diluent in the preheating tank from which the air bubbles are discharged is sent to the counting tank of the blood cell analyzer for white blood cell counting and classification.
  • the blood cell analyzer may include the process of exiting from sleep, starting to work, and entering sleep, which is determined according to the actual demand for blood analysis, because of the uncertain timing of the arrival of blood samples .
  • the blood cell analyzer (counting pool) can enter the dormant state after completing the analysis of a batch of samples, and wait for the next batch of samples to temporarily exit the dormant state before working. Since the blood cell analyzer enters the dormant state from the working state, the preheating pool still continues to heat the diluent to prepare for the next batch of sample analysis.
  • the dilution of the preheating pool is The gas precipitated in the liquid increases with the increase of the dormant time.
  • the air bubbles in the preheating pool of the blood cell analyzer can be discharged first (as described in step S110 ), and then perform white blood cell counting and classification based on the diluted solution discharged from the bubbles (as described in step S120). In this way, it is avoided that the white blood cell count and classification results are affected by the air bubble problem, and the reliability of blood analysis results is improved.
  • the process of discharging bubbles in the preheating tank in step S110 may include: sucking out part of the diluent in the preheating tank to make the bubbles stay in the preheating tank and be discharged. Empty top; fill the preheating pool with new diluent to refill the preheating pool and exhaust the gas at the top of the preheating pool.
  • the bubbles on the top of the diluent are separated from the diluent to the cavity obtained by drawing out part of the diluent at the top of the preheating tank by sucking out part of the diluent from the preheating tank; after the gas-liquid separation, Fill the preheating tank with new diluent so that the preheating tank is refilled with diluent.
  • the gas in the cavity at the top of the preheating tank will be discharged as the new diluent is added, thereby realizing the The air bubbles are expelled.
  • the bubbles in the preheating pool can be automatically discharged through the following process: the syringe of the blood cell analyzer is connected to the preheating pool, and the syringe is connected to the preheating pool.
  • Part of the diluent is sucked out of the preheating tank to empty the top of the preheating tank; the syringe is discharged from the diluent sucked from the preheating tank through the first pipeline; the syringe is connected to the The diluent barrel is in communication, and new diluent is sucked from the diluent barrel by the syringe to fill the preheating pool with new diluent; and the syringe is in communication with the preheating pool, And the new diluent sucked from the diluent barrel is filled into the preheating tank by the syringe to refill the preheating tank.
  • FIG. 2A shows the initial state of the blood cell analyzer, that is, the state of the blood cell analyzer after exiting from dormancy.
  • the diluent in the preheating tank is delivered by the syringe to the preheating tank after drawing the diluent from the diluent barrel.
  • the syringe is connected with the first valve SV1
  • the preheating tank and the diluent barrel are both connected with the second valve SV2.
  • the first valve SV1 includes terminals a1, c1, and d1, and the second valve SV2 includes terminals a2, c2, and d2.
  • the syringe When terminals a1 and d1 are connected and terminals a2 and c2 are connected, the syringe is connected to the preheating tank; when terminals a1 and d1 are connected and terminals a2 and d2 are connected, the syringe is connected to the diluent barrel; when terminals a1 and c1 are connected When connected, the syringe is in communication with the first pipeline T1.
  • the first pipeline T1 may be a pipeline for discharging liquid in the annotator.
  • the preheating pool is also connected to a third valve SV3, and the third valve SV3 includes terminals a3, c3, and d3.
  • the preheating pool is connected with the second pipeline T2, so that the diluent in the preheating pool is transported to the counting pool via the second pipeline T2; when the terminals a3 and c3 are connected, the preheating pool It is connected with the third pipeline T3, so that the diluent is delivered to the bottom of the counting tank through the third pipeline T3 and discharged from the counting tank.
  • Fig. 2B shows a schematic diagram of a syringe of the blood cell analyzer sucking a part of the diluent from the preheating tank.
  • the terminal a1 of the first valve SV1 is connected to the terminal d1
  • the terminal a2 of the second valve SV2 is connected to the terminal c2, so that the syringe is connected to the preheating tank.
  • the syringe can suck a part of the diluent from the preheating tank, so that the top of the preheating tank is emptied, and the liquid path flows as shown by the arrow in FIG. 2B.
  • FIG. 2C shows a schematic diagram of the syringe of the blood cell analyzer discharging the diluent sucked from the preheating tank through the first pipeline.
  • the terminal a1 of the first valve SV1 is connected to the terminal c1, so that the syringe communicates with the first pipeline T1. Based on this connection, the syringe can discharge the diluent sucked from the preheating tank through the first pipe T1, and the liquid path flows as shown by the arrow in FIG. 2C.
  • Fig. 2D shows a schematic diagram of the syringe of the blood cell analyzer drawing new diluent from the diluent barrel for refilling the preheating tank.
  • the terminal a1 of the first valve SV1 is connected to the terminal d1
  • the terminal a2 of the second valve SV2 is connected to the terminal d2, so that the syringe is connected to the diluent barrel.
  • the syringe can suck new diluent from the diluent barrel for refilling it into the preheating tank, and the flow direction of the liquid path is as shown by the arrow in FIG. 2D.
  • Fig. 2E shows a schematic diagram of the syringe of the blood cell analyzer withdrawing new diluent from the diluent barrel to fill the preheating tank.
  • the terminal a1 of the first valve SV1 is connected to the terminal d1
  • the terminal a2 of the second valve SV2 is connected to the terminal c2, so that the syringe is connected to the preheating tank.
  • the syringe can replenish the new diluent sucked from the diluent barrel into the preheating tank, so that the preheating tank is refilled with diluent, so that the process of expelling the bubbles in the preheating tank is realized.
  • the flow direction is shown by the arrow in Figure 2E.
  • the amount of diluent sucked by the syringe from the preheating tank may depend on the dormancy time of the counting tank. length. As mentioned above, the longer the dormant time of the counting cell, the more gas will be released in the preheating cell, so the syringe can draw more diluent from the preheating cell; the shorter the dormant time of the counting cell, the more gas will be released in the preheating cell. The less gas is released, so the syringe can draw relatively less diluent from the preheating tank.
  • the specific amount of diluent drawn by the syringe from the preheating tank can be set based on experience or calculated based on a certain calculation method, which is not limited in this application.
  • the process of discharging bubbles in the preheating tank according to the embodiment of the present invention may also be any other suitable process, and is not limited to the foregoing process.
  • the counting and classification of white blood cells performed by the counting cell can avoid affecting the white blood cell counting and classification results due to bubble problems, and improve the reliability of blood analysis results.
  • the analysis method 300 of the blood cell analyzer may include the following steps:
  • step S310 after the blood cell analyzer exits dormancy, the air bubbles in the preheating pool of the blood cell analyzer are discharged.
  • step S320 the diluent in the preheating pool from which the bubbles have been discharged is sent to the counting pool of the blood cell analyzer for white blood cell counting and classification.
  • step S330 after the blood cell analyzer enters dormancy again, the temperature of the preheating pool is lowered.
  • step S310 and step S320 are respectively similar to step S110 and step S120 in the method 100 described above with reference to FIG. 1, and will not be repeated here for brevity.
  • the analysis method 300 of the blood cell analyzer further includes step S330, which describes the operation of the blood cell analyzer (the counting cell) after it enters dormancy again. It should be understood that after the blood cell analyzer has processed a batch of samples, it can be timed, and the sleep state can be re-entered after the time reaches a certain time.
  • step S330 after the blood cell analyzer enters dormancy again, the temperature of the preheating pool can be lowered, so as to reduce the amount of gas released by the hematology analyzer during dormancy, thereby reducing the need to remove the gas from the next time after exiting dormancy.
  • the amount of air bubbles discharged from the preheating tank is to reduce the diluent that needs to be sucked out of the preheating tank after the next exit from dormancy, thereby reducing the diluent that needs to be added to the preheating tank, thereby improving the reliability of blood analysis at the same time cut costs.
  • the analysis method 400 of the blood cell analyzer may include the following steps:
  • step S410 after the blood cell analyzer exits dormancy, the air bubbles in the preheating pool of the blood cell analyzer are discharged.
  • step S420 the diluent in the preheating tank from which the air bubbles are discharged is sent to the counting tank of the blood cell analyzer for white blood cell counting and classification.
  • step S430 after the blood cell analyzer enters dormancy again, the time for the blood cell analyzer to enter dormancy is counted.
  • step S440 the temperature of the preheating pool is adjusted based on the length of time during which the blood cell analyzer enters the dormancy, so as to reduce the gas precipitated by the diluent in the preheating pool during the dormancy period of the blood cell analyzer the amount.
  • step S410 and step S420 are respectively similar to step S110 and step S120 in the method 100 described above with reference to FIG. 1, and for the sake of brevity, they will not be repeated here.
  • the analysis method 400 of the blood cell analyzer further includes step S430 and step S440, which describe the operation of the blood cell analyzer (the counting cell) after it enters dormancy again.
  • the time when the blood cell analyzer enters dormancy can be timed, and based on the timed time (that is, the time when the blood cell analyzer enters dormancy The length of time) is used to adjust the temperature of the preheating pool to reduce the amount of gas precipitated by the diluent in the preheating pool during the dormant period of the blood cell analyzer.
  • the temperature of the preheating pool is adjusted based on the length of time the blood cell analyzer enters sleep.
  • the temperature of the preheating pool can be higher ( For example, the temperature is lower than when it just enters the dormant state); when the blood cell analyzer enters the dormant state for a long time, the temperature of the preheating pool can be lower (for example, compared with the time when the blood cell analyzer enters the dormant state). It is much lower than the temperature), which can reduce the amount of gas released by the blood cell analyzer during the hibernation process, and at the same time reduce the time for the preheating pool to be heated after the next exit from the hibernation (reheating to the required reaction temperature), Improve the processing efficiency of the blood cell analyzer.
  • adjusting the temperature of the preheating pool based on the length of time the blood cell analyzer enters sleep in step S440 may further include: gradually adjusting the temperature of the preheating pool as the time during which the blood cell analyzer enters sleep becomes longer. Lower the temperature of the preheating bath.
  • the temperature of the preheating cell is 42°C, and the temperature of the preheating cell can be set to the first temperature (for example, 40°C) when the blood cell analyzer just enters dormancy;
  • the temperature of the preheating pool is set to a second temperature (for example, 38° C.), and the second temperature is lower than the first temperature
  • the temperature of the preheating pool is set to a third temperature (for example, 35° C.), and the second time period is greater than the first time period For a period of time, the third temperature is lower than the second temperature.
  • first temperature can be set arbitrarily according to requirements, and are not limited to include the above
  • the temperature and time period can also include more or less temperature/time periods, which can be adjusted arbitrarily according to requirements.
  • the analysis method 500 of the blood cell analyzer may include the following steps:
  • step S510 after the blood cell analyzer exits the dormancy, the air bubbles in the preheating pool of the blood cell analyzer are discharged.
  • step S520 the diluent in the preheating pool after the bubbles have been discharged is sent to the counting pool of the blood cell analyzer for white blood cell counting and classification.
  • step S530 after the blood cell analyzer enters dormancy, the time when the blood cell analyzer enters dormancy is counted, and the air bubbles in the preheating pool are discharged at predetermined time intervals based on the timing.
  • step S510 and step S520 are respectively similar to step S110 and step S120 in the method 100 described above with reference to FIG. 1.
  • the analysis method 500 of the blood cell analyzer further includes step S530, which describes the operation after the blood cell analyzer (the counting cell) enters sleep.
  • step S530 describes the operation after the blood cell analyzer (the counting cell) enters sleep.
  • the time when the hematology analyzer enters dormancy can be counted, and the air bubbles in the preheating pool can be periodically discharged based on the timing.
  • the bubbles in the preheating pool are discharged at regular intervals, not necessarily after the next exit from dormancy.
  • step S530 does not need to be sequenced after steps S510 and S520, or step S530 itself can directly form an independent solution for achieving bubble discharge from the preheating pool.
  • step S530 may also be combined with the method described above in conjunction with FIG. 3 and FIG. 4, and for the sake of brevity, it will not be repeated here.
  • the aforementioned analysis method of the blood cell analyzer may further include (not shown in the figure): each time the blood cell analyzer performs white blood cell counting and classification, the The air bubbles in the preheating pool of the blood cell analyzer are discharged.
  • the air bubbles in the preheating pool of the blood cell analyzer are discharged before each white blood cell count and classification.
  • a general hematology analyzer can time after processing a batch of samples, and enter the dormant state after the timer reaches a certain time.
  • the hematology analyzer does not enter the dormant state after processing a batch of samples. From time to time, a new batch of samples comes, and at this time, the air bubbles in the preheating pool of the blood cell analyzer can be discharged before processing a new batch of samples, which can further improve the processing accuracy of the blood cell analyzer.
  • the above exemplarily shows the analysis method of the blood cell analyzer according to the embodiment of the present invention.
  • the analysis method of the blood cell analyzer according to the embodiment of the present invention discharges the air bubbles in the preheating pool after the blood cell analyzer exits the dormancy, so that the blood cell analyzer is based on the white blood cell count and classification.
  • the bubble-removing diluent does not affect the white blood cell count and classification results due to bubble problems, and improves the reliability of blood analysis results.
  • the analysis method of the blood cell analyzer according to the embodiment of the present invention reduces the temperature of the preheating pool after the blood cell analyzer enters dormancy again, which can improve the reliability of blood analysis while saving costs.
  • the time when the blood cell analyzer enters dormancy is counted, and the temperature of the preheating pool is adjusted based on the time counted. It can reduce the amount of gas released during the dormancy process of the hematology analyzer, and at the same time reduce the time for heating the preheating pool after exiting dormancy next time, and improve the processing efficiency of the hematology analyzer.
  • the air bubbles in the preheating pool of the blood cell analyzer are discharged, which can further improve The processing accuracy of the blood cell analyzer.
  • Fig. 6 shows a schematic block diagram of a blood cell analyzer 600 according to an embodiment of the present application.
  • the blood cell analyzer 600 includes a sampling component 610, a preheating tank 620, a hemolytic agent supply component 630, a counting tank 640 and a bubble discharge device 650.
  • the sampling component 610 is used to send the collected blood sample into the counting pool 640.
  • the preheating tank 620 is used to heat the diluent before entering the counting tank 640.
  • the hemolytic agent supply component 630 is used to supply the hemolytic agent to the counting pool 640.
  • the counting pool 640 is used to perform hemolysis treatment on the diluted blood, and perform white blood cell counting and classification.
  • the bubble discharging device 650 is used for discharging the bubbles in the preheating tank 620 after the counting cell 640 exits the dormancy, so as to be used for the counting cell 640 to count and classify white blood cells based on the diluent of the discharged bubbles.
  • the blood cell analyzer 600 can implement the analysis method 100 of the blood cell analyzer described above with reference to FIG. 1.
  • the blood cell analyzer 600 may include processes such as exiting sleep, starting work, and entering sleep, which are determined according to the actual demand for blood analysis, because the arrival of blood samples is uncertain. Therefore, the blood cell analyzer 600 (counting pool 640) can enter the dormant state after completing the analysis of a batch of samples, and wait for the next batch of samples to temporarily exit the dormant state before working. Since the blood cell analyzer 600 enters the dormant state from the working state, the preheating pool 620 continues to heat the diluent to prepare for the next batch of sample analysis.
  • the preheating pool 620 is The gas precipitated in the diluent increases with the increase of the dormant time.
  • the bubble discharge device 650 can discharge the bubbles in the preheating tank 620, and then the counting cell 640 is based on the discharged bubbles. Diluent for white blood cell count and classification. In this way, it is avoided that the white blood cell count and classification results are affected due to the air bubble problem, and the reliability of the blood analysis results is improved.
  • the bubble-removing device 650 can also be used to deliver the diluent to the preheating tank 620 and the counting tank 640. That is to say, the bubble-eliminating device 650 may be a syringe, and the syringe can be realized by sucking out part of the diluent in the preheating pool 620 and adding new diluent to the preheating pool 620 to refill the preheating pool 620. The air bubbles in the preheating tank 620 are discharged.
  • a syringe is used to suck out part of the diluent from the preheating tank 620 so that the bubbles on the top of the diluent are separated from the diluent to the cavity at the top of the preheating tank 620 by drawing out part of the diluent; After separation, the syringe fills the preheating pool 620 with new diluent, so that the preheating pool 620 is refilled with the diluent, and the gas in the top cavity of the preheating pool 620 is discharged as the new diluent is added, thereby achieving In order to expel the air bubbles in the preheating pool 620.
  • the blood cell analyzer 600 may further include a first valve, a second valve, and a diluent barrel for supplying diluent (none of which is shown in FIG. 6, and you can refer to FIGS. 2A to 2E).
  • a first valve a second valve
  • a diluent barrel for supplying diluent (none of which is shown in FIG. 6, and you can refer to FIGS. 2A to 2E).
  • the syringe can be removed from the preheating pool 620 based on the communication between the first valve and the second valve and the communication between the second valve and the preheating pool 620.
  • a part of the diluent is sucked out of the preheating tank 620 so that the top of the preheating tank 620 is emptied; then, the syringe may be removed from the preheating tank 620 based on the communication between the first valve and the first pipeline
  • the diluent sucked from the preheating tank 620 is discharged through the first pipeline; then, the syringe can be based on the communication between the first valve and the second valve and the connection between the second valve and the diluent barrel
  • the new diluent is sucked out from the diluent barrel to be used to replenish the new diluent into the preheating tank 620; finally, the syringe may be based on the connection between the first valve and the second valve
  • the communication and the communication between the second valve and the preheating tank 620 make up the new diluent sucked from the diluent barrel into the preheating tank 620 to refill the prehe
  • the amount of diluent drawn by the syringe from the preheating tank 620 may depend on the length of the dormant time of the counting tank 640. As mentioned above, the longer the dormancy time of the counting cell 640 is, the more gas will be released in the preheating cell 620, so the syringe can draw more diluent from the preheating cell 620; the shorter the dormancy time of the counting cell 640, The less gas is released in the preheating tank 620, so the syringe can draw relatively less diluent from the preheating tank 620.
  • the specific amount of diluent drawn by the syringe from the preheating pool 620 can be set based on experience or calculated based on a certain calculation method, which is not limited in this application.
  • the bubble ejecting device 650 according to the embodiment of the present invention can also be any other suitable structure, and the bubble ejecting process can also be any other suitable process, and is not limited to the above-mentioned structure and process.
  • the counting and classification of white blood cells performed by the counting pool 640 can avoid affecting the white blood cell counting and classification results due to bubble problems, and improve the reliability of the blood analysis results.
  • the blood cell analyzer 700 includes a sampling component 710, a preheating tank 720, a hemolytic agent supply component 730, a counting cell 740, a bubble discharge device 750 and a preheating tank temperature adjusting device 760.
  • the sampling component 710 is used to send the collected blood sample into the counting pool 740.
  • the preheating tank 720 is used to heat the diluent before entering the counting tank 740.
  • the hemolytic agent supply component 730 is used to supply the hemolytic agent to the counting cell 740.
  • the counting pool 740 is used to perform hemolysis treatment on the diluted blood, and perform white blood cell count and classification.
  • the bubble discharging device 750 is used for discharging the bubbles in the preheating tank 720 after the counting cell 740 exits the dormancy, so as to be used for the counting cell 740 to count and classify white blood cells based on the diluent of the discharged bubbles.
  • the preheating pool temperature adjusting device 760 is used to reduce the temperature of the preheating pool 720 after the counting pool 740 enters dormancy again.
  • the blood cell analyzer 700 can implement the analysis method 300 of the blood cell analyzer described above with reference to FIG. 3.
  • the sampling component 710, the preheating tank 720, the hemolytic agent supply component 730, the counting tank 740, and the bubble discharge device 750 are respectively the same as the sampling component 610, the preheating tank 620, and the hemolytic agent supply component 630, described above with reference to FIG.
  • the counting cell 640 is similar to the bubble discharging device 650, and for the sake of brevity, it will not be repeated here.
  • the blood cell analyzer 700 further includes a preheating bath temperature adjustment device 760.
  • the preheating pool temperature adjusting device 760 can lower the temperature of the preheating pool 720, so as to reduce the amount of gas released by the counting pool 740 during the dormancy process, thereby reducing the need to resume the preheating after exiting the dormancy next time.
  • the amount of air bubbles discharged from the hot pool 720 is to reduce the diluent that needs to be drawn from the preheating pool 720 after the next exit from dormancy, thereby reducing the diluent that needs to be added to the preheating pool 720, thereby improving the reliability of blood analysis At the same time save costs.
  • the blood cell analyzer 800 includes a sampling component 810, a preheating pool 820, a hemolytic agent supply component 830, a counting pool 840, a bubble discharge device 850, a preheating pool temperature adjusting device 860, and a sleep time timely device 870.
  • the sampling component 810 is used to send the collected blood sample into the counting pool 840.
  • the preheating tank 820 is used for heating the diluent before entering the counting tank 840.
  • the hemolytic agent supply component 830 is used to supply the hemolytic agent to the counting cell 840.
  • the counting pool 840 is used to perform hemolysis treatment on the diluted blood, and perform white blood cell counting and classification.
  • the bubble discharging device 850 is used for discharging the bubbles in the preheating tank 820 after the counting cell 840 exits the dormancy, so as to be used for the counting cell 840 to count and classify white blood cells based on the diluent of the discharged bubbles.
  • the sleep time timing device 870 is used to count the time when the counting pool 840 enters sleep after the counting pool 840 enters sleep again.
  • the preheating pool temperature adjusting device 860 is used to adjust the temperature of the preheating pool 820 based on the length of time the counting pool 840 enters sleep, so as to reduce the temperature of the counting pool 840 in the preheating pool 820 during the sleep period. The amount of gas released by the diluent.
  • the blood cell analyzer 800 can implement the analysis method 400 of the blood cell analyzer described above with reference to FIG. 4.
  • the sampling component 810, the preheating pool 820, the hemolytic agent supply component 830, the counting pool 840, and the bubble discharge device 850 are respectively the same as the sampling component 610, the preheating pool 620, and the hemolytic agent supply component 630 described above with reference to FIG.
  • the counting tank 640 is similar to the bubble-removing device 650, and for the sake of brevity, it will not be repeated here.
  • the blood cell analyzer 800 further includes a warm-up tank temperature adjustment device 860 and a sleep time timer 870.
  • the sleep time timing device 870 can count the time when the counting pool 840 enters sleep, and the preheating pool temperature adjusting device 860 is based on the time counted (that is, the counting pool 840 enters sleep time).
  • the length of time is used to adjust the temperature of the preheating pool 820 to reduce the amount of gas precipitated by the diluent in the preheating pool 820 during the dormant period of the counting pool 840.
  • the preheating pool temperature adjusting device 860 adjusts the temperature of the preheating pool 820 based on the length of time the counting pool 840 enters sleep.
  • the counting pool 840 When the counting pool 840 enters sleep for a short time, it can make the preheating pool
  • the temperature of 820 is a bit higher (for example, the temperature is lowered less than when it just enters the dormant state); when the counting pool 840 enters dormancy for a long time, the temperature of the preheating pool 820 can be lower (for example, The temperature is lower than when it just enters the dormant state), this can reduce the amount of gas precipitated by the counting cell 840 during the dormant process, and at the same time reduce the time for the preheating pool 820 to be heated after the next exit from dormancy (reheating) Return the required reaction temperature) to improve the processing efficiency of the blood cell analyzer 800.
  • adjusting the temperature of the preheating pool 820 by the preheating pool temperature adjusting device 860 based on the length of time the counting pool 840 enters sleep may further include: as the time for the counting pool 840 to enter sleep becomes longer The temperature of the preheating tank 820 is gradually lowered. Further, for example, when the counting pool 840 is working, the temperature of the preheating pool is 42°C, and the temperature of the preheating pool 820 can be set to the first temperature (for example, 40°C) when the counting pool 840 just enters sleep.
  • the temperature of the preheating pool 820 is set to a second temperature (for example, 38° C.), and the second temperature is lower than the first Temperature; and when the time period during which the counting pool 840 enters sleep reaches a second time period, the temperature of the preheating pool 820 is set to a third temperature (for example, 35° C.), and the second time period is greater than the In the first time period, the third temperature is lower than the second temperature.
  • first temperature can be set arbitrarily according to requirements, and are not limited to include the above
  • the temperature and time period can also include more or less temperature/time periods, which can be adjusted arbitrarily according to requirements.
  • the blood cell analyzer 900 includes a sampling component 910, a preheating tank 920, a hemolytic agent supply component 930, a counting tank 940, a bubble exhausting device 950 and a sleep time timing device 970.
  • the sampling component 910 is used to send the collected blood sample into the counting pool 940.
  • the preheating tank 920 is used to heat the diluent before entering the counting tank 940.
  • the hemolytic agent supply component 930 is used to supply the hemolytic agent to the counting cell 940.
  • the counting pool 940 is used to perform hemolysis treatment on the diluted blood, and perform white blood cell count and classification.
  • the bubble discharging device 950 is used for discharging the bubbles in the preheating tank 920 after the counting cell 940 exits the dormancy, so as to be used for the counting cell 940 to count and classify white blood cells based on the diluent of the discharged bubbles.
  • the sleep time counting device 970 is used to count the time when the counting pool 940 enters sleep after the counting pool 940 enters sleep.
  • the bubble ejection device 950 is also used to exhaust the bubbles in the preheating pool 920 at predetermined time intervals based on the timing of the dormancy time timing device 970 after the counting pool 940 enters sleep.
  • the blood cell analyzer 900 can implement the analysis method 500 of the blood cell analyzer described above with reference to FIG. 5.
  • the sampling component 910, the preheating pool 920, the hemolytic agent supply component 930, the counting pool 940, and the bubble discharge device 950 are respectively the same as the sampling component 610, the preheating pool 620, and the hemolytic agent supply component 630 described above with reference to FIG.
  • the counting tank 640 is similar to the bubble-removing device 650, and for the sake of brevity, it will not be repeated here.
  • the blood cell analyzer 900 further includes a sleep time timer 970.
  • the time when the counting pool 940 enters dormancy can be counted, and the bubbles in the preheating pool 920 can be periodically discharged based on the timing.
  • the air bubbles in the preheating pool 920 are discharged at regular intervals, and the air bubbles are not necessarily discharged after the next exit from hibernation.
  • the logic is simple, easy to implement, and it is possible to exit hibernation next time. At this time, the air bubbles in the preheating pool 920 have been discharged, so that the reliability of blood cell analysis can be improved, and the processing efficiency of the blood cell analyzer can be further improved.
  • the aforementioned bubble discharging device of the blood cell analyzer can also be used to expel the bubbles in the preheating pool before each time the counting and sorting of white blood cells is performed in the counting pool.
  • the air bubbles in the preheating cell are discharged before each white blood cell counting and classification.
  • a general counting pool can be timed after processing a batch of samples, and the counting pool enters the dormant state when the timing reaches a certain time.
  • the counting pool may not enter the dormant state after processing a batch of samples. Come to a new batch of samples.
  • the bubbles in the preheating tank can be discharged before processing a new batch of samples, which can further improve the processing accuracy of the blood cell analyzer.
  • the blood cell analyzer according to the embodiment of the present invention discharges the air bubbles in the preheating pool after the blood cell analyzer exits dormancy, so that the blood cell analyzer performs white blood cell counting and classification based on the removal of air bubbles. Diluent, so it will not affect the white blood cell count and classification results due to air bubbles, and improve the reliability of blood analysis results.
  • the blood cell analyzer according to the embodiment of the present invention lowers the temperature of the preheating pool after the blood cell analyzer enters dormancy again, which can improve the reliability of blood analysis while saving costs.
  • the blood cell analyzer counts the time when the blood cell analyzer enters dormancy after the blood cell analyzer enters dormancy again, and adjusts the temperature of the preheating pool based on the timed time, which can reduce The amount of gas released during the dormancy process of the hematology analyzer reduces the time required to heat the preheating pool after the next exit from dormancy, thereby improving the processing efficiency of the hematology analyzer. Further, the blood cell analyzer according to the embodiment of the present invention exhausts the air bubbles in the preheating pool of the blood cell analyzer every time before the blood cell analyzer performs white blood cell counting and classification, which can further improve the blood cell analysis. The processing accuracy of the instrument.
  • the disclosed device and method may be implemented in other ways.
  • the device embodiments described above are only illustrative.
  • the division of the units is only a logical function division, and there may be other divisions in actual implementation, for example, multiple units or components may be combined or It can be integrated into another device, or some features can be ignored or not implemented.
  • the various component embodiments of the present application may be implemented by hardware, or by software modules running on one or more processors, or by a combination of them.
  • a microprocessor or a digital signal processor (DSP) may be used in practice to implement some or all of the functions of some modules according to the embodiments of the present application.
  • This application can also be implemented as a device program (for example, a computer program and a computer program product) for executing part or all of the methods described herein.
  • Such a program for realizing the present application may be stored on a computer-readable medium, or may have the form of one or more signals.
  • Such a signal can be downloaded from an Internet website, or provided on a carrier signal, or provided in any other form.

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Abstract

一种血液细胞分析仪的分析方法（100）和血液细胞分析仪，该血液细胞分析仪的分析方法（100）包括：在血液细胞分析仪退出休眠之后，将血液细胞分析仪的预热池中的气泡排出（S110）；以及将经排出气泡的预热池中的稀释液送入到血液细胞分析仪的计数池，以用于进行白细胞计数和分类（S120）。该血液细胞分析仪的分析方法（100）和该血液细胞分析仪通过在血液细胞分析仪退出休眠之后将预热池中的气泡排出，使得血液细胞分析仪在进行白细胞计数和分类时基于已除气泡的稀释液，因而不会因气泡问题导致影响白细胞计数和分类结果，提高了血液分析结果的可靠性。

Description

血液细胞分析仪的分析方法和血液细胞分析仪
说明书
技术领域
本申请涉及血液细胞分析仪技术领域，更具体地涉及一种血液细胞分析仪的分析方法和血液细胞分析仪。
背景技术
血液细胞分析仪在进行血样分析过程中，如果需要实现阻抗法的四分类或五分类，通常需要对稀释液进行加热，达到四分类或五分类所需要的反应温度。稀释液经过低温到高温的变化，溶解在稀释液中的气体会因温差变化而析出，以气泡的形式存在于预热池中，且气体的析出量会随着血液细胞分析仪休眠时间的延长而增加。由于气体与液体的可压缩性不同，气泡的产生会导致血液细胞分析仪的注射器在样本采集、稀释液加入等定量过程的不稳定，从而影响血液细胞分析仪的测量结果。
发明内容
为了解决上述问题而提出了本申请。本申请提供一种血液细胞分析仪的分析方案，其通过在血液细胞分析仪退出休眠之后将预热池中的气泡排出，使得血液细胞分析仪在进行白细胞计数和分类时基于已除气泡的稀释液，因而不会因气泡问题导致影响白细胞计数和分类结果，提高了血液分析结果的可靠性。下面简要描述本申请提出的血液细胞分析仪的分析方案，更多细节将在后续结合附图在具体实施方式中加以描述。
本申请一方面，提供了一种血液细胞分析仪的分析方法，所述分析方法包括：在所述血液细胞分析仪退出休眠之后，将所述血液细胞分析仪的预热池中的气泡排出；以及将经排出气泡的所述预热池中的稀释液送入到所述血液细胞分析仪的计数池，以用于进行白细胞计数和分类。
在本申请的一个实施例中，所述预热池中的气泡排出过程包括：吸出所述预热池中的部分稀释液以使得所述气泡滞留在所述预热池被排空的顶 部；以及向所述预热池补入新的稀释液以重新充满所述预热池而排出所述预热池顶部的气体。
在本申请的一个实施例中，所述预热池中的气泡排出过程进一步包括：所述血液细胞分析仪的注射器与所述预热池连通，并由所述注射器从所述预热池中吸出部分稀释液以使得所述预热池的顶部排空；所述注射器将从所述预热池中吸出的稀释液经由第一管路排出；所述注射器与所述稀释液桶连通，并由所述注射器从所述稀释液桶吸出新的稀释液以用于向所述预热池补入新的稀释液；以及所述注射器与所述预热池连通，并由所述注射器将从所述稀释液桶吸出的新的稀释液补入所述预热池，以重新充满所述预热池。
在本申请的一个实施例中，所述分析方法还包括：在所述血液细胞分析仪重新进入休眠之后，降低所述预热池的温度。
在本申请的一个实施例中，所述分析方法还包括：在所述血液细胞分析仪重新进入休眠之后，对所述血液细胞分析仪进入休眠的时间计时；以及基于所述血液细胞分析仪进入休眠的时间的长短来调整所述预热池的温度，以减少所述血液细胞分析仪在休眠期间所述预热池中的稀释液析出的气体量。
在本申请的一个实施例中，所述基于所述血液细胞分析仪进入休眠的时间的长短来调整所述预热池的温度，包括：随着所述血液细胞分析仪进入休眠的时间变长而逐渐调低所述预热池的温度。
在本申请的一个实施例中，所述基于所述血液细胞分析仪进入休眠的时间的长短来调整所述预热池的温度，包括：在所述血液细胞分析仪刚进入休眠时，将所述预热池的温度设置为第一温度；在所述血液细胞分析仪进入休眠的时间段达到第一时间段时，将所述预热池的温度设置为第二温度，所述第二温度低于所述第一温度；以及在所述血液细胞分析仪进入休眠的时间段达到第二时间段时，将所述预热池的温度设置为第三温度，所述第二时间段大于所述第一时间段，所述第三温度低于所述第二温度。
在本申请的一个实施例中，所述分析方法还包括：在所述血液细胞分析仪进入休眠之后，对所述血液细胞分析仪进入休眠的时间计时，并基于所述计时每隔预定时间间隔就将所述预热池中的气泡排出。
在本申请的一个实施例中，所述分析方法还包括：在所述血液细胞分析仪每次进行白细胞计数和分类之前，均将所述血液细胞分析仪的预热池中的气泡排出。
本申请另一方面，提供了一种血液细胞分析仪，所述血液细胞分析仪包括：采样组件，用于将采集的血样送入计数池；预热池，用于对进入所述计数池之前的稀释液进行加热；溶血剂供应组件，用于向所述计数池中供应溶血剂；所述计数池，用于对稀释后的血液进行溶血处理，并进行白细胞计数和分类；以及排气泡装置，用于在所述计数池退出休眠之后将所述预热池中的气泡排出，排出气泡的稀释液进行白细胞计数和分类。
在本申请的一个实施例中，所述排气泡装置还用于向所述预热池和所述计数池输送所述稀释液。
在本申请的一个实施例中，所述排气泡装置是注射器，所述注射器通过将所述预热池中的部分稀释液吸出并向所述预热池补入新的稀释液以重新充满所述预热池而实现将所述预热池中的气泡排出。
在本申请的一个实施例中，所述血液细胞分析仪还包括第一阀门、第二阀门和提供稀释液的稀释液桶，其中：所述注射器基于所述第一阀门与所述第二阀门的连通以及所述第二阀门与所述预热池的连通而从所述预热池中吸出部分稀释液以使得所述预热池的顶部排空；所述注射器基于所述第一阀门与所述第一管路的连通而将从所述预热池中吸出的稀释液经由所述第一管路排出；所述注射器基于所述第一阀门与所述第二阀门的连通以及所述第二阀门与所述稀释液桶的连通而从所述稀释液桶吸出新的稀释液以用于向所述预热池补入新的稀释液；所述注射器基于所述第一阀门与所述第二阀门的连通以及所述第二阀门与所述预热池的连通而将从所述稀释液桶吸出的新的稀释液补入所述预热池，以重新充满所述预热池。
在本申请的一个实施例中，所述血液细胞分析仪还包括：预热池温度调整装置，用于在所述计数池重新进入休眠之后，降低所述预热池的温度。
在本申请的一个实施例中，所述血液细胞分析仪还包括：休眠时间计时装置，用于在所述计数池重新进入休眠之后，对所述计数池进入休眠的时间计时；以及预热池温度调整装置，用于基于所述计数池进入休眠的时间的长短来调整所述预热池的温度，以减少所述计数池在休眠期间所述预 热池中的稀释液析出的气体量。
在本申请的一个实施例中，所述预热池温度调整装置对所述预热池的温度的调整包括：随着所述计数池进入休眠的时间变长而逐渐调低所述预热池的温度。
在本申请的一个实施例中，所述预热池温度调整装置对所述预热池的温度的调整包括：在所述计数池刚进入休眠时，将所述预热池的温度设置为第一温度；在所述计数池进入休眠的时间段达到第一时间段时，将所述预热池的温度设置为第二温度，所述第二温度低于所述第一温度；以及在所述计数池进入休眠的时间段达到第二时间段时，将所述预热池的温度设置为第三温度，所述第二时间段大于所述第一时间段，所述第三温度低于所述第二温度。
在本申请的一个实施例中，所述血液细胞分析仪还包括：休眠时间计时装置，用于在所述计数池进入休眠之后，对所述计数池进入休眠的时间计时；并且所述排气泡装置还用于：在所述计数池进入休眠之后，基于所述休眠时间计时装置的计时每隔预定时间间隔就将所述预热池中的气泡排出。
在本申请的一个实施例中，所述排气泡装置还用于：在所述计数池每次进行白细胞计数和分类之前，均将所述预热池中的气泡排出。
根据本申请实施例的血液细胞分析仪的分析方法和血液细胞分析仪通过在血液细胞分析仪退出休眠之后将预热池中的气泡排出，使得血液细胞分析仪在进行白细胞计数和分类时基于已除气泡的稀释液，因而不会因气泡问题导致影响白细胞计数和分类结果，提高了血液分析结果的可靠性。
附图说明
图1示出根据本申请一个实施例的血液细胞分析仪的分析方法的示意性流程图；
图2A到图2E示出根据本申请实施例的血液细胞分析仪的分析方法中预热池中气泡的排出过程的示意图；
图3示出根据本申请另一个实施例的血液细胞分析仪的分析方法的示意性流程图；
图4示出根据本申请再一个实施例的血液细胞分析仪的分析方法的示意性流程图；
图5示出根据本申请又一个实施例的血液细胞分析仪的分析方法的示意性流程图；
图6示出根据本申请一个实施例的血液细胞分析仪的示意性框图；
图7示出根据本申请另一个实施例的血液细胞分析仪的示意性框图；
图8示出根据本申请再一个实施例的血液细胞分析仪的示意性框图；以及
图9示出根据本申请又一个实施例的血液细胞分析仪的示意性框图。
具体实施方式
为了使得本申请的目的、技术方案和优点更为明显，下面将参照附图详细描述根据本申请的示例实施例。显然，所描述的实施例仅仅是本申请的一部分实施例，而不是本申请的全部实施例，应理解，本申请不受这里描述的示例实施例的限制。基于本申请中描述的本申请实施例，本领域技术人员在没有付出创造性劳动的情况下所得到的所有其它实施例都应落入本申请的保护范围之内。
在下文的描述中，给出了大量具体的细节以便提供对本申请更为彻底的理解。然而，对于本领域技术人员而言显而易见的是，本申请可以无需一个或多个这些细节而得以实施。在其他的例子中，为了避免与本申请发生混淆，对于本领域公知的一些技术特征未进行描述。
应当理解的是，本申请能够以不同形式实施，而不应当解释为局限于这里提出的实施例。相反地，提供这些实施例将使公开彻底和完全，并且将本申请的范围完全地传递给本领域技术人员。
在此使用的术语的目的仅在于描述具体实施例并且不作为本申请的限制。在此使用时，单数形式的“一”、“一个”和“所述/该”也意图包括复数形式，除非上下文清楚指出另外的方式。还应明白术语“组成”和/或“包括”，当在该说明书中使用时，确定所述特征、整数、步骤、操作、元件和/或部件的存在，但不排除一个或更多其它的特征、整数、步骤、操作、元件、部件和/或组的存在或添加。在此使用时，术语“和/或”包括相 关所列项目的任何及所有组合。
为了彻底理解本申请，将在下列的描述中提出详细的步骤以及详细的结构，以便阐释本申请提出的技术方案。本申请的较佳实施例详细描述如下，然而除了这些详细描述外，本申请还可以具有其他实施方式。
首先，从总体上描述本申请的发明背景。在本申请的实施例中的血液细胞分析仪包括预热池和计数池，预热池将稀释液加热到一定温度，然后经加热的稀释液送入计数池对血样进行稀释后，由计数池对稀释后的血液进行溶血处理并进行白细胞计数和分类。基于经加热的稀释液稀释后的血液进行白细胞计数和分类可以获得更好的计数和分类结果。尤其对于动物血液细胞分析，加热稀释液是实现动物白细胞四分类和五分类的关键。本申请中的血液细胞分析仪优选的为动物血液细胞分析仪。然而，这引入了新的技术问题，即稀释液在被预热池加热的过程中，由于经历温差变化，导致溶解在稀释液中的气体被析出而以气泡的形式存在于预热池中，且随着血液细胞分析仪(计数池)的休眠时间的延长，气体的析出量将不断增加，这将导致血液细胞分析仪在样本采集、试剂加入等定量过程不稳定，从而影响测量结果，如前所述的。基于对该技术问题的发现，本申请提供了如下将详细描述的血液细胞分析仪的分析方法来解决该技术问题。
下面，首先参考图1描述根据本申请一个实施例的血液细胞分析仪的分析方法100。如图1所示，血液细胞分析仪的分析方法100可以包括如下步骤：
在步骤S110，在血液细胞分析仪退出休眠之后，将所述血液细胞分析仪的预热池中的气泡排出。
在步骤S120，将经排出气泡的所述预热池中的稀释液送入到所述血液细胞分析仪的计数池，以用于进行白细胞计数和分类。
在本申请的实施例中，血液细胞分析仪可以包括退出休眠、开始工作、进入休眠这样的过程，这是根据对血液分析的实际需求来确定的，因为血液样本到来的不定时性不确定性，血液细胞分析仪(的计数池)在完成一批样本的分析后可进入休眠状态，待到下批样本来临时退出休眠状态再进行工作。由于血液细胞分析仪在从工作状态进入休眠状态后，预热池仍然继续对稀释液进行加热而为下批样本分析做准备，因此，在血液细胞分析 仪的休眠过程中，预热池的稀释液中析出的气体随休眠时间的增加而增加。基于此，在根据本申请实施例的血液细胞分析仪的分析方法100中，当血液细胞分析仪退出休眠之后，可以先将血液细胞分析仪的预热池中的气泡排出(如步骤S110所述的)，再基于经排出气泡的稀释液进行白细胞计数和分类(如步骤S120所述的)。这样就避免了因气泡问题导致影响白细胞计数和分类结果，提高了血液分析结果的可靠性。
在本申请的实施例中，步骤S110中的将预热池中的气泡排出的过程可以包括：吸出所述预热池中的部分稀释液以使得所述气泡滞留在所述预热池被排空的顶部；向所述预热池补入新的稀释液以重新充满所述预热池而排出所述预热池顶部的气体。在该实施例中，通过从预热池中吸出部分稀释液而使得稀释液顶部的气泡从稀释液中分离到预热池顶部因抽出部分稀释液而得到的空腔；在气液分离后，向预热池补入新的稀释液，使得预热池重新充满稀释液，预热池顶部空腔的气体随着新的稀释液的补入而被排出，从而实现了将预热池中的气泡排出。
进一步地，从血液细胞分析仪的具体结构出发，可以通过如下过程来实现将预热池中的气泡自动排出：所述血液细胞分析仪的注射器与所述预热池连通，并由所述注射器从所述预热池中吸出部分稀释液以使得所述预热池的顶部排空；所述注射器将从所述预热池中吸出的稀释液经由第一管路排出；所述注射器与所述稀释液桶连通，并由所述注射器从所述稀释液桶吸出新的稀释液以用于向所述预热池补入新的稀释液；以及所述注射器与所述预热池连通，并由所述注射器将从所述稀释液桶吸出的新的稀释液补入所述预热池，以重新充满所述预热池。
为了使得上述的描述更清楚，下面参照图2A到图2E来描述将预热池中的气泡排出的过程。
首先，图2A示出血液细胞分析仪的初始状态，即退出休眠之后血液细胞分析仪的状态。如图2A所示的，在初始状态下，预热池顶部聚集着气泡，计数池中尚未被输送稀释液。其中，预热池中的稀释液是注射器通过从稀释液桶中抽出稀释液后输送到预热池中的。注射器与第一阀门SV1连接，预热池和稀释液桶均与第二阀门SV2连接。第一阀门SV1包括端子a1、c1和d1，第二阀门SV2包括端子a2、c2和d2。当端子a1和d1连接 且端子a2和c2连接时，注射器与预热池之间连通；当端子a1和d1连接且端子a2和d2连接时，注射器与稀释液桶之间连通；当端子a1和c1连接时，注射器与第一管路T1连通。第一管路T1可以是用于排出注释器中液体的管路。此外，预热池还与第三阀门SV3连接，第三阀门SV3包括端子a3、c3和d3。当端子a3和d3连接时，预热池与第二管路T2连通，从而经由第二管路T2将预热池中的稀释液输送到计数池；当端子a3和c3连接时，预热池与第三管路T3连通，从而将稀释液经由第三管路T3输送到计数池底部并排出计数池。
图2B示出血液细胞分析仪的注射器从预热池中吸出部分稀释液的示意图。如图2B所示的，第一阀门SV1的端子a1连接端子d1，第二阀门SV2的端子a2连接端子c2，从而使得注射器与预热池连通。基于该连通，注射器可从预热池吸出部分稀释液，使得预热池的顶部被排空，液路流向如图2B中的箭头所示的。
图2C示出血液细胞分析仪的注射器将从预热池中吸出的稀释液经由第一管路排出的示意图。如图2C所示的，第一阀门SV1的端子a1连接端子c1，使得注射器与第一管路T1连通。基于该连通，注射器可将从预热池中吸出的稀释液经由第一管路T1排出，液路流向如图2C中的箭头所示的。
图2D示出血液细胞分析仪的注射器从稀释液桶抽取新的稀释液以用于补入预热池的示意图。如图2D所示的，第一阀门SV1的端子a1连接端子d1，第二阀门SV2的端子a2连接端子d2，从而使得注射器与稀释液桶连通。基于该连通，注射器可从稀释液桶吸出新的稀释液，以用于补入到预热池中，液路流向如图2D中的箭头所示的。
图2E示出血液细胞分析仪的注射器将从稀释液桶中抽取新的稀释液补入预热池的示意图。如图2E所示的，第一阀门SV1的端子a1连接端子d1，第二阀门SV2的端子a2连接端子c2，从而使得注射器与预热池连通。基于该连通，注射器可将从稀释液桶吸出的新的稀释液补入到预热池中，以使预热池重新充满稀释液，如此实现了将预热池中的气泡排出的过程，液路流向如图2E中的箭头所示的。
以上参照图2A到2E描述了根据本发明实施例的将预热池中的气泡排 出的示例性过程，应理解，注射器从预热池中吸出的稀释液的量可以取决于计数池休眠时间的长短。如前所述的，计数池休眠时间越长，预热池中析出的气体越多，因而注射器从预热池中吸出的稀释液可以相对较多；计数池休眠时间越短，预热池中析出的气体越少，因而注射器从预热池中吸出的稀释液可以相对较少。基于该指导思想，注射器从预热池中吸出的稀释液的具体量可以根据经验设置，也可以根据一定的计算方法计算得到，本申请对此不作限制。此外，还应理解，根据本发明实施例的将预热池中的气泡排出的过程也可以是其他任何合适的过程，而不仅限于上述过程。
基于经排出气泡的稀释液，计数池所进行的白细胞的计数和分类可以避免因气泡问题导致影响白细胞计数和分类结果，提高了血液分析结果的可靠性。
下面参照图3描述根据本申请另一实施例的血液细胞分析仪的分析方法300的示意性流程图。如图3所示，血液细胞分析仪的分析方法300可以包括如下步骤：
在步骤S310，在血液细胞分析仪退出休眠之后，将所述血液细胞分析仪的预热池中的气泡排出。
在步骤S320，将经排出气泡的所述预热池中的稀释液送入到所述血液细胞分析仪的计数池，以用于进行白细胞计数和分类。
在步骤S330，在所述血液细胞分析仪重新进入休眠之后，降低所述预热池的温度。
其中，步骤S310和步骤S320分别与前文中参照图1描述的方法100中的步骤S110和步骤S120类似，为了简洁，此处不再赘述。与图1描述的方法100不同的是，血液细胞分析仪的分析方法300还包括步骤S330，在该步骤描述了血液细胞分析仪(的计数池)重新进入休眠之后的操作。应理解，在血液细胞分析仪处理完一批样本后，可以进行计时，在计时达到一定时间后重新进入休眠状态。如步骤S330所述的，待血液细胞分析仪重新进入休眠之后，可以降低预热池的温度，这样可以减少血液细胞分析仪在休眠过程中析出的气体量，从而减少下次退出休眠之后需从预热池中排出的气泡量，即减少下次退出休眠之后需从预热池中吸出的稀释液，从而减少需向预热池中补入的稀释液，进而在提高血液分析可靠性的同时节 省成本。
下面参照图4描述根据本申请再一实施例的血液细胞分析仪的分析方法400的示意性流程图。如图4所示，血液细胞分析仪的分析方法400可以包括如下步骤：
在步骤S410，在血液细胞分析仪退出休眠之后，将所述血液细胞分析仪的预热池中的气泡排出。
在步骤S420，将经排出气泡的所述预热池中的稀释液送入到所述血液细胞分析仪的计数池，以用于进行白细胞计数和分类。
在步骤S430，在所述血液细胞分析仪重新进入休眠之后，对所述血液细胞分析仪进入休眠的时间计时。
在步骤S440，基于所述血液细胞分析仪进入休眠的时间的长短来调整所述预热池的温度，以减少所述血液细胞分析仪在休眠期间所述预热池中的稀释液析出的气体量。
其中，步骤S410和步骤S420分别与前文中参照图1描述的方法100中的步骤S110和步骤S120类似，为了简洁，此处不再赘述。与图1描述的方法100不同的是，血液细胞分析仪的分析方法400还包括步骤S430和步骤S440，其描述了血液细胞分析仪(的计数池)重新进入休眠之后的操作。如步骤S430和步骤S440所述的，待血液细胞分析仪重新进入休眠之后，可以对血液细胞分析仪进入休眠的时间进行计时，并基于该计时的时间(即所述血液细胞分析仪进入休眠的时间的长短)来调整预热池的温度，以减少所述血液细胞分析仪在休眠期间所述预热池中的稀释液析出的气体量。在该实施例中，基于血液细胞分析仪进入休眠的时间的长短来调整预热池的温度，当血液细胞分析仪进入休眠的时间的较短时，可以使得预热池的温度偏高一些(例如与刚进入休眠状态时相比温度调低得较少)；当血液细胞分析仪进入休眠的时间的较长时，可以使得预热池的温度偏低一些(例如与刚进入休眠状态时相比温度调低得较多)，这样能够在减少血液细胞分析仪在休眠过程中析出的气体量的同时减少下次退出休眠之后应对预热池加热的时间(重新加热回需要的反应温度)，提高血液细胞分析仪的处理效率。
示例性地，步骤S440中基于所述血液细胞分析仪进入休眠的时间的 长短来调整所述预热池的温度可以进一步包括：随着所述血液细胞分析仪进入休眠的时间变长而逐渐调低所述预热池的温度。进一步地，例如，计数池工作时，预热池温度为42℃，可以在所述血液细胞分析仪刚进入休眠时，将所述预热池的温度设置为第一温度(例如40℃)；在所述血液细胞分析仪进入休眠的时间段达到第一时间段时，将所述预热池的温度设置为第二温度(例如38℃)，所述第二温度低于所述第一温度；以及在所述血液细胞分析仪进入休眠的时间段达到第二时间段时，将所述预热池的温度设置为第三温度(例如35℃)，所述第二时间段大于所述第一时间段，所述第三温度低于所述第二温度。上述示例均可以实现在减少血液细胞分析仪在休眠过程中析出的气体量的同时减少下次退出休眠之后应对预热池加热的时间，提高血液细胞分析仪的处理效率。应理解，前述的“第一温度”、“第二温度”、“第三温度”、“第一时间段”和“第二时间段”均可以根据需求任意设置，并且也不限于包括上述的温度和时间段，还可以包括更多或更少的温度/时间段，可以根据需求任意调整。
下面参照图5描述根据本申请又一实施例的血液细胞分析仪的分析方法500的示意性流程图。如图5所示，血液细胞分析仪的分析方法500可以包括如下步骤：
在步骤S510，在血液细胞分析仪退出休眠之后，将所述血液细胞分析仪的预热池中的气泡排出。
在步骤S520，将经排出气泡的所述预热池中的稀释液送入到所述血液细胞分析仪的计数池，以用于进行白细胞计数和分类。
在步骤S530，在所述血液细胞分析仪进入休眠之后，对所述血液细胞分析仪进入休眠的时间计时，并基于所述计时每隔预定时间间隔就将所述预热池中的气泡排出。
其中，步骤S510和步骤S520分别与前文中参照图1描述的方法100中的步骤S110和步骤S120类似，为了简洁，此处不再赘述。与图1描述的方法100不同的是，血液细胞分析仪的分析方法500还包括步骤S530，该步骤描述了血液细胞分析仪(的计数池)进入休眠之后的操作。如步骤S530所述的，待血液细胞分析仪进入休眠之后，可以对血液细胞分析仪进入休眠的时间进行计时，并基于该计时定时地排出预热池中的气泡。在该 实施例中，每隔一定的时间就对预热池中的气泡进行排出处理，而不一定是在下次退出休眠后再排出气泡，逻辑简单，易于实现，且可能实现在下次退出休眠时预热池中气泡已经排出，因而能在提高血液细胞分析可靠性的同时进一步提高血液细胞分析仪的处理效率。应理解，步骤S530也可以无需排序在步骤S510和S520之后，也可以直接由步骤S530自身组成一个独立的实现预热池气泡排出的方案。此外，步骤S530也可以与前文结合图3和图4描述的方法相结合，为了简洁，此处不再赘述。
在本申请进一步的实施例中，前述的血液细胞分析仪的分析方法还可以包括(未在图中示出)：在所述血液细胞分析仪每次进行白细胞计数和分类之前，均将所述血液细胞分析仪的预热池中的气泡排出。在该实施例中，可以不考虑血液细胞分析仪是否退出休眠或进入休眠，而是在每次进行白细胞计数和分类之前均将所述血液细胞分析仪的预热池中的气泡排出。例如，一般血液细胞分析仪在处理完一批样本后，可以计时，在计时达到一定时间才进入休眠状态，在该实施例中，可能在血液细胞分析仪在处理完一批样本后未进入休眠时又来一批新的样本，此时可以将所述血液细胞分析仪的预热池中的气泡排出后再进行新一批样本的处理，这样可以进一步提高血液细胞分析仪的处理精度。
以上示例性地示出了根据本发明实施例的血液细胞分析仪的分析方法。基于上面的描述，根据本发明实施例的血液细胞分析仪的分析方法通过在血液细胞分析仪退出休眠之后将预热池中的气泡排出，使得血液细胞分析仪在进行白细胞计数和分类时基于已除气泡的稀释液，因而不会因气泡问题导致影响白细胞计数和分类结果，提高了血液分析结果的可靠性。此外，根据本发明实施例的血液细胞分析仪的分析方法在血液细胞分析仪重新进入休眠之后降低预热池的温度，可以在提高血液分析可靠性的同时节省成本。此外，根据本发明实施例的血液细胞分析仪的分析方法在血液细胞分析仪重新进入休眠之后对血液细胞分析仪进入休眠的时间进行计时，并基于该计时的时间来调整预热池的温度，能够在减少血液细胞分析仪在休眠过程中析出的气体量的同时减少下次退出休眠之后应对预热池加热的时间，提高血液细胞分析仪的处理效率。进一步地，根据本发明实施例的血液细胞分析仪的分析方法在血液细胞分析仪每次进行白细胞计数和 分类之前，均将所述血液细胞分析仪的预热池中的气泡排出，可以进一步提高血液细胞分析仪的处理精度。
下面结合图6描述根据本申请另一方面提供的血液细胞分析仪。图6示出了根据本申请实施例的血液细胞分析仪600的示意性框图。如图6所示的，血液细胞分析仪600包括采样组件610、预热池620、溶血剂供应组件630、计数池640和排气泡装置650。其中，采样组件610用于将采集的血样送入计数池640。预热池620用于对进入所述计数池640之前的稀释液进行加热。溶血剂供应组件630用于向所述计数池640中供应溶血剂。计数池640用于对稀释后的血液进行溶血处理，并进行白细胞计数和分类。排气泡装置650用于在计数池640退出休眠之后将预热池620中的气泡排出，以用于计数池640基于经排出气泡的稀释液进行白细胞计数和分类。血液细胞分析仪600可以实施前文结合图1描述的血液细胞分析仪的分析方法100。
在本申请的实施例中，血液细胞分析仪600可以包括退出休眠、开始工作、进入休眠这样的过程，这是根据对血液分析的实际需求来确定的，因为血液样本到来的不定时性不确定性，血液细胞分析仪600(的计数池640)在完成一批样本的分析后可进入休眠状态，待到下批样本来临时退出休眠状态再进行工作。由于血液细胞分析仪600在从工作状态进入休眠状态后，预热池620仍然继续对稀释液进行加热而为下批样本分析做准备，因此，在计数池640的休眠过程中，预热池620的稀释液中析出的气体随休眠时间的增加而增加。基于此，在根据本申请实施例的血液细胞分析仪600中，当计数池640退出休眠之后，排气泡装置650可以将预热池620中的气泡排出，再由计数池640基于经排出气泡的稀释液进行白细胞计数和分类。这样就避免了因气泡问题导致影响白细胞计数和分类结果，提高了血液分析结果的可靠性。
在本申请的实施例中，排气泡装置650还可以用于向预热池620和计数池640输送稀释液。也就是说，排气泡装置650可以是注射器，该注射器可以通过将预热池620中的部分稀释液吸出并向预热池620补入新的稀释液以重新充满预热池620而实现将预热池620中的气泡排出。在该实施例中，通过注射器从预热池620中吸出部分稀释液而使得稀释液顶部的气 泡从稀释液中分离到预热池620顶部因抽出部分稀释液而得到的空腔；在气液分离后，注射器向预热池620补入新的稀释液，使得预热池620重新充满稀释液，预热池620顶部空腔的气体随着新的稀释液的补入而被排出，从而实现了将预热池620中的气泡排出。
进一步地，血液细胞分析仪600还可以包括第一阀门、第二阀门和提供稀释液的稀释液桶(均未在图6中示出，可以参见图2A到图2E)。为了将实现将预热池620中的气泡排出，首先，所述注射器可以基于所述第一阀门与所述第二阀门的连通以及所述第二阀门与所述预热池620的连通而从所述预热池620中吸出部分稀释液以使得所述预热池620的顶部排空；接着，所述注射器可以基于所述第一阀门与所述第一管路的连通而将从所述预热池620中吸出的稀释液经由所述第一管路排出；然后，所述注射器可以基于所述第一阀门与所述第二阀门的连通以及所述第二阀门与所述稀释液桶的连通而从所述稀释液桶吸出新的稀释液以用于向所述预热池620补入新的稀释液；最后，所述注射器可以基于所述第一阀门与所述第二阀门的连通以及所述第二阀门与所述预热池620的连通而将从所述稀释液桶吸出的新的稀释液补入所述预热池620，以重新充满所述预热池620。可以参照图2A到图2E结合前文的描述理解血液细胞分析仪600的排气泡装置650的结构以及操作，为了简洁，此处不再赘述。
应理解，注射器从预热池620中吸出的稀释液的量可以取决于计数池640休眠时间的长短。如前所述的，计数池640休眠时间越长，预热池620中析出的气体越多，因而注射器从预热池620中吸出的稀释液可以相对较多；计数池640休眠时间越短，预热池620中析出的气体越少，因而注射器从预热池620中吸出的稀释液可以相对较少。基于该指导思想，注射器从预热池620中吸出的稀释液的具体量可以根据经验设置，也可以根据一定的计算方法计算得到，本申请对此不作限制。此外，还应理解，根据本发明实施例的排气泡装置650也可以是其他任何合适的结构，其排气泡过程也可以是其他任何合适的过程，而不仅限于上述结构和过程。
基于经排出气泡的稀释液，计数池640所进行的白细胞的计数和分类可以避免因气泡问题导致影响白细胞计数和分类结果，提高了血液分析结果的可靠性。
下面参照图7描述根据本申请另一实施例的血液细胞分析仪700的示意性框图。如图7所示的，血液细胞分析仪700包括采样组件710、预热池720、溶血剂供应组件730、计数池740、排气泡装置750和预热池温度调整装置760。其中，采样组件710用于将采集的血样送入计数池740。预热池720用于对进入所述计数池740之前的稀释液进行加热。溶血剂供应组件730用于向所述计数池740中供应溶血剂。计数池740用于对稀释后的血液进行溶血处理，并进行白细胞计数和分类。排气泡装置750用于在计数池740退出休眠之后将预热池720中的气泡排出，以用于计数池740基于经排出气泡的稀释液进行白细胞计数和分类。预热池温度调整装置760用于在计数池740重新进入休眠之后，降低预热池720的温度。血液细胞分析仪700可以实施前文结合图3描述的血液细胞分析仪的分析方法300。
其中，采样组件710、预热池720、溶血剂供应组件730、计数池740和排气泡装置750分别与前文中参照图6描述的采样组件610、预热池620、溶血剂供应组件630、计数池640和排气泡装置650类似，为了简洁，此处不再赘述。与图6描述的血液细胞分析仪600不同的是，血液细胞分析仪700还包括预热池温度调整装置760。待计数池740重新进入休眠之后，预热池温度调整装置760可以降低预热池720的温度，这样可以减少计数池740在休眠过程中析出的气体量，从而减少下次退出休眠之后需从预热池720中排出的气泡量，即减少下次退出休眠之后需从预热池720中吸出的稀释液，从而减少需向预热池720中补入的稀释液，进而在提高血液分析可靠性的同时节省成本。
下面参照图8描述根据本申请再一实施例的血液细胞分析仪800的示意性框图。如图8所示的，血液细胞分析仪800包括采样组件810、预热池820、溶血剂供应组件830、计数池840、排气泡装置850、预热池温度调整装置860和休眠时间及时装置870。其中，采样组件810用于将采集的血样送入计数池840。预热池820用于对进入所述计数池840之前的稀释液进行加热。溶血剂供应组件830用于向所述计数池840中供应溶血剂。计数池840用于对稀释后的血液进行溶血处理，并进行白细胞计数和分类。排气泡装置850用于在计数池840退出休眠之后将预热池820中的气泡排 出，以用于计数池840基于经排出气泡的稀释液进行白细胞计数和分类。休眠时间计时装置870用于在所述计数池840重新进入休眠之后，对所述计数池840进入休眠的时间计时。预热池温度调整装置860用于基于所述计数池840进入休眠的时间的长短来调整所述预热池820的温度，以减少所述计数池840在休眠期间所述预热池820中的稀释液析出的气体量。血液细胞分析仪800可以实施前文结合图4描述的血液细胞分析仪的分析方法400。
其中，采样组件810、预热池820、溶血剂供应组件830、计数池840和排气泡装置850、分别与前文中参照图6描述的采样组件610、预热池620、溶血剂供应组件630、计数池640和排气泡装置650类似，为了简洁，此处不再赘述。与图6描述的血液细胞分析仪600不同的是，血液细胞分析仪800还包括预热池温度调整装置860和休眠时间计时装置870。待计数池840重新进入休眠之后，休眠时间计时装置870可以对计数池840进入休眠的时间进行计时，并由预热池温度调整装置860基于该计时的时间(即所述计数池840进入休眠的时间的长短)来调整预热池820的温度，以减少所述计数池840在休眠期间所述预热池820中的稀释液析出的气体量。在该实施例中，预热池温度调整装置860基于计数池840进入休眠的时间的长短来调整预热池820的温度，当计数池840进入休眠的时间的较短时，可以使得预热池820的温度偏高一些(例如与刚进入休眠状态时相比温度调低得较少)；当计数池840进入休眠的时间的较长时，可以使得预热池820的温度偏低一些(例如与刚进入休眠状态时相比温度调低得较多)，这样能够在减少计数池840在休眠过程中析出的气体量的同时减少下次退出休眠之后应对预热池820加热的时间(重新加热回需要的反应温度)，提高血液细胞分析仪800的处理效率。
示例性地，预热池温度调整装置860基于所述计数池840进入休眠的时间的长短来调整所述预热池820的温度可以进一步包括：随着所述计数池840进入休眠的时间变长而逐渐调低所述预热池820的温度。进一步地，例如，计数池840工作时，预热池温度为42℃，可以在所述计数池840刚进入休眠时，将所述预热池820的温度设置为第一温度(例如40℃)；在所述计数池840进入休眠的时间段达到第一时间段时，将所述预热池820 的温度设置为第二温度(例如38℃)，所述第二温度低于所述第一温度；以及在所述计数池840进入休眠的时间段达到第二时间段时，将所述预热池820的温度设置为第三温度(例如35℃)，所述第二时间段大于所述第一时间段，所述第三温度低于所述第二温度。上述示例均可以实现在减少计数池840在休眠过程中析出的气体量的同时减少下次退出休眠之后应对预热池820加热的时间，提高血液细胞分析仪的处理效率。应理解，前述的“第一温度”、“第二温度”、“第三温度”、“第一时间段”和“第二时间段”均可以根据需求任意设置，并且也不限于包括上述的温度和时间段，还可以包括更多或更少的温度/时间段，可以根据需求任意调整。
下面参照图9描述根据本申请又一实施例的血液细胞分析仪900的示意性框图。如图9所示的，血液细胞分析仪900包括采样组件910、预热池920、溶血剂供应组件930、计数池940、排气泡装置950和休眠时间计时装置970。其中，采样组件910用于将采集的血样送入计数池940。预热池920用于对进入所述计数池940之前的稀释液进行加热。溶血剂供应组件930用于向所述计数池940中供应溶血剂。计数池940用于对稀释后的血液进行溶血处理，并进行白细胞计数和分类。排气泡装置950用于在计数池940退出休眠之后将预热池920中的气泡排出，以用于计数池940基于经排出气泡的稀释液进行白细胞计数和分类。休眠时间计时装置970用于在所述计数池940进入休眠之后，对所述计数池940进入休眠的时间计时。并且，所述排气泡装置950还用于在所述计数池940进入休眠之后，基于所述休眠时间计时装置970的计时每隔预定时间间隔就将所述预热池920中的气泡排出。血液细胞分析仪900可以实施前文结合图5描述的血液细胞分析仪的分析方法500。
其中，采样组件910、预热池920、溶血剂供应组件930、计数池940、和排气泡装置950分别与前文中参照图6描述的采样组件610、预热池620、溶血剂供应组件630、计数池640和排气泡装置650类似，为了简洁，此处不再赘述。与图6描述的血液细胞分析仪600不同的是，血液细胞分析仪900还包括休眠时间计时装置970。待计数池940进入休眠之后，可以对计数池940进入休眠的时间进行计时，并基于该计时定时地排出预热池920中的气泡。在该实施例中，每隔一定的时间就对预热池920中的气泡进行 排出处理，而不一定是在下次退出休眠后再排出气泡，逻辑简单，易于实现，且可能实现在下次退出休眠时预热池920中气泡已经排出，因而能在提高血液细胞分析可靠性的同时进一步提高血液细胞分析仪的处理效率。
在本申请进一步的实施例中，前述的血液细胞分析仪的排气泡装置还可以用于：在计数池每次进行白细胞计数和分类之前，均将预热池中的气泡排出。在该实施例中，可以不考虑计数池是否退出休眠或进入休眠，而是在每次进行白细胞计数和分类之前均将预热池中的气泡排出。例如，一般计数池在处理完一批样本后，可以计时，在计时达到一定时间计数池才进入休眠状态，在该实施例中，可能在计数池在处理完一批样本后未进入休眠时又来一批新的样本，此时可以将预热池中的气泡排出后再进行新一批样本的处理，这样可以进一步提高血液细胞分析仪的处理精度。
以上示例性地示出了根据本发明实施例的血液细胞分析仪。基于上面的描述，根据本发明实施例的血液细胞分析仪通过在血液细胞分析仪退出休眠之后将预热池中的气泡排出，使得血液细胞分析仪在进行白细胞计数和分类时基于已除气泡的稀释液，因而不会因气泡问题导致影响白细胞计数和分类结果，提高了血液分析结果的可靠性。此外，根据本发明实施例的血液细胞分析仪在血液细胞分析仪重新进入休眠之后降低预热池的温度，可以在提高血液分析可靠性的同时节省成本。此外，根据本发明实施例的血液细胞分析仪在血液细胞分析仪重新进入休眠之后对血液细胞分析仪进入休眠的时间进行计时，并基于该计时的时间来调整预热池的温度，能够在减少血液细胞分析仪在休眠过程中析出的气体量的同时减少下次退出休眠之后应对预热池加热的时间，提高血液细胞分析仪的处理效率。进一步地，根据本发明实施例的血液细胞分析仪在血液细胞分析仪每次进行白细胞计数和分类之前，均将所述血液细胞分析仪的预热池中的气泡排出，可以进一步提高血液细胞分析仪的处理精度。
尽管这里已经参考附图描述了示例实施例，应理解上述示例实施例仅仅是示例性的，并且不意图将本申请的范围限制于此。本领域普通技术人员可以在其中进行各种改变和修改，而不偏离本申请的范围和精神。所有这些改变和修改意在被包括在所附权利要求所要求的本申请的范围之内。
本领域普通技术人员可以意识到，结合本文中所公开的实施例描述的 各示例的单元及算法步骤，能够以电子硬件、或者计算机软件和电子硬件的结合来实现。这些功能究竟以硬件还是软件方式来执行，取决于技术方案的特定应用和设计约束条件。专业技术人员可以对每个特定的应用来使用不同方法来实现所描述的功能，但是这种实现不应认为超出本申请的范围。
在本申请所提供的几个实施例中，应该理解到，所揭露的设备和方法，可以通过其它的方式实现。例如，以上所描述的设备实施例仅仅是示意性的，例如，所述单元的划分，仅仅为一种逻辑功能划分，实际实现时可以有另外的划分方式，例如多个单元或组件可以结合或者可以集成到另一个设备，或一些特征可以忽略，或不执行。
在此处所提供的说明书中，说明了大量具体细节。然而，能够理解，本申请的实施例可以在没有这些具体细节的情况下实践。在一些实例中，并未详细示出公知的方法、结构和技术，以便不模糊对本说明书的理解。
类似地，应当理解，为了精简本申请并帮助理解各个发明方面中的一个或多个，在对本申请的示例性实施例的描述中，本申请的各个特征有时被一起分组到单个实施例、图、或者对其的描述中。然而，并不应将该本申请的方法解释成反映如下意图：即所要求保护的本申请要求比在每个权利要求中所明确记载的特征更多的特征。更确切地说，如相应的权利要求书所反映的那样，其发明点在于可以用少于某个公开的单个实施例的所有特征的特征来解决相应的技术问题。因此，遵循具体实施方式的权利要求书由此明确地并入该具体实施方式，其中每个权利要求本身都作为本申请的单独实施例。
本领域的技术人员可以理解，除了特征之间相互排斥之外，可以采用任何组合对本说明书(包括伴随的权利要求、摘要和附图)中公开的所有特征以及如此公开的任何方法或者设备的所有过程或单元进行组合。除非另外明确陈述，本说明书(包括伴随的权利要求、摘要和附图)中公开的每个特征可以由提供相同、等同或相似目的的替代特征来代替。
此外，本领域的技术人员能够理解，尽管在此所述的一些实施例包括其它实施例中所包括的某些特征而不是其它特征，但是不同实施例的特征的组合意味着处于本申请的范围之内并且形成不同的实施例。例如，在权 利要求书中，所要求保护的实施例的任意之一都可以以任意的组合方式来使用。
本申请的各个部件实施例可以以硬件实现，或者以在一个或者多个处理器上运行的软件模块实现，或者以它们的组合实现。本领域的技术人员应当理解，可以在实践中使用微处理器或者数字信号处理器(DSP)来实现根据本申请实施例的一些模块的一些或者全部功能。本申请还可以实现为用于执行这里所描述的方法的一部分或者全部的装置程序(例如，计算机程序和计算机程序产品)。这样的实现本申请的程序可以存储在计算机可读介质上，或者可以具有一个或者多个信号的形式。这样的信号可以从因特网网站上下载得到，或者在载体信号上提供，或者以任何其他形式提供。
应该注意的是上述实施例对本申请进行说明而不是对本申请进行限制，并且本领域技术人员在不脱离所附权利要求的范围的情况下可设计出替换实施例。在权利要求中，不应将位于括号之间的任何参考符号构造成对权利要求的限制。本申请可以借助于包括有若干不同元件的硬件以及借助于适当编程的计算机来实现。在列举了若干装置的单元权利要求中，这些装置中的若干个可以是通过同一个硬件项来具体体现。单词第一、第二、以及第三等的使用不表示任何顺序。可将这些单词解释为名称。
以上所述，仅为本申请的具体实施方式或对具体实施方式的说明，本申请的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本申请揭露的技术范围内，可轻易想到变化或替换，都应涵盖在本申请的保护范围之内。本申请的保护范围应以权利要求的保护范围为准。

Claims (19)

  1. 一种血液细胞分析仪的分析方法，其特征在于，所述分析方法包括：
    在所述血液细胞分析仪退出休眠之后，将所述血液细胞分析仪的预热池中的气泡排出；以及
    将经排出气泡的所述预热池中的稀释液送入到所述血液细胞分析仪的计数池，以用于进行白细胞计数和分类。
  2. 根据权利要求1所述的分析方法，其特征在于，所述预热池中的气泡排出过程包括：
    吸出所述预热池中的部分稀释液以使得所述气泡滞留在所述预热池被排空的顶部；以及
    向所述预热池补入新的稀释液以重新充满所述预热池而排出所述预热池顶部的气体。
  3. 根据权利要求2所述的分析方法，其特征在于，所述预热池中的气泡排出过程进一步包括：
    所述血液细胞分析仪的注射器与所述预热池连通，并由所述注射器从所述预热池中吸出部分稀释液以使得所述预热池的顶部排空；
    所述注射器将从所述预热池中吸出的稀释液经由第一管路排出；
    所述注射器与所述稀释液桶连通，并由所述注射器从所述稀释液桶吸出新的稀释液以用于向所述预热池补入新的稀释液；以及
    所述注射器与所述预热池连通，并由所述注射器将从所述稀释液桶吸出的新的稀释液补入所述预热池，以重新充满所述预热池。
  4. 根据权利要求1-3中的任一项所述的分析方法，其特征在于，所述分析方法还包括：
    在所述血液细胞分析仪重新进入休眠之后，降低所述预热池的温度。
  5. 根据权利要求1-3中的任一项所述的分析方法，其特征在于，所述分析方法还包括：
    在所述血液细胞分析仪重新进入休眠之后，对所述血液细胞分析仪进入休眠的时间计时；以及
    基于所述血液细胞分析仪进入休眠的时间的长短来调整所述预热池 的温度，以减少所述血液细胞分析仪在休眠期间所述预热池中的稀释液析出的气体量。
  6. 根据权利要求5所述的分析方法，其特征在于，所述基于所述血液细胞分析仪进入休眠的时间的长短来调整所述预热池的温度，包括：
    随着所述血液细胞分析仪进入休眠的时间变长而逐渐调低所述预热池的温度。
  7. 根据权利要求6所述的分析方法，其特征在于，所述基于所述血液细胞分析仪进入休眠的时间的长短来调整所述预热池的温度，包括：
    在所述血液细胞分析仪刚进入休眠时，将所述预热池的温度设置为第一温度；
    在所述血液细胞分析仪进入休眠的时间段达到第一时间段时，将所述预热池的温度设置为第二温度，所述第二温度低于所述第一温度；以及
    在所述血液细胞分析仪进入休眠的时间段达到第二时间段时，将所述预热池的温度设置为第三温度，所述第二时间段大于所述第一时间段，所述第三温度低于所述第二温度。
  8. 根据权利要求1-3和5-7中的任一项所述的分析方法，其特征在于，所述分析方法还包括：
    在所述血液细胞分析仪进入休眠之后，对所述血液细胞分析仪进入休眠的时间计时，并基于所述计时每隔预定时间间隔就将所述预热池中的气泡排出。
  9. 根据权利要求1-3和5-7中的任一项所述的分析方法，其特征在于，所述分析方法还包括：
    在所述血液细胞分析仪每次进行白细胞计数和分类之前，均将所述血液细胞分析仪的预热池中的气泡排出。
  10. 一种血液细胞分析仪，其特征在于，所述血液细胞分析仪包括：
    采样组件，用于将采集的血样送入计数池；
    预热池，用于对进入所述计数池之前的稀释液进行加热；
    溶血剂供应组件，用于向所述计数池中供应溶血剂；
    所述计数池，用于对稀释后的血液进行溶血处理，并进行白细胞计数和分类；以及
    排气泡装置，用于在所述计数池退出休眠之后将所述预热池中的气泡排出，排出气泡的稀释液用于进行白细胞计数和分类。
  11. 根据权利要求10所述的血液细胞分析仪，其特征在于，所述排气泡装置还用于向所述预热池和所述计数池输送所述稀释液。
  12. 根据权利要求11所述的血液细胞分析仪，其特征在于，所述排气泡装置是注射器，所述注射器通过将所述预热池中的部分稀释液吸出并向所述预热池补入新的稀释液以重新充满所述预热池而实现将所述预热池中的气泡排出。
  13. 根据权利要求12所述的血液细胞分析仪，其特征在于，所述血液细胞分析仪还包括第一阀门、第二阀门和提供稀释液的稀释液桶，其中：
    所述注射器基于所述第一阀门与所述第二阀门的连通以及所述第二阀门与所述预热池的连通而从所述预热池中吸出部分稀释液以使得所述预热池的顶部排空；
    所述注射器基于所述第一阀门与所述第一管路的连通而将从所述预热池中吸出的稀释液经由所述第一管路排出；
    所述注射器基于所述第一阀门与所述第二阀门的连通以及所述第二阀门与所述稀释液桶的连通而从所述稀释液桶吸出新的稀释液以用于向所述预热池补入新的稀释液；
    所述注射器基于所述第一阀门与所述第二阀门的连通以及所述第二阀门与所述预热池的连通而将从所述稀释液桶吸出的新的稀释液补入所述预热池，以重新充满所述预热池。
  14. 根据权利要求10-13中的任一项所述的血液细胞分析仪，其特征在于，所述血液细胞分析仪还包括：
    预热池温度调整装置，用于在所述计数池重新进入休眠之后，降低所述预热池的温度。
  15. 根据权利要求10-13中的任一项所述的血液细胞分析仪，其特征在于，所述血液细胞分析仪还包括：
    休眠时间计时装置，用于在所述计数池重新进入休眠之后，对所述计数池进入休眠的时间计时；以及
    预热池温度调整装置，用于基于所述计数池进入休眠的时间的长短来 调整所述预热池的温度，以减少所述计数池在休眠期间所述预热池中的稀释液析出的气体量。
  16. 根据权利要求15所述的血液细胞分析仪，其特征在于，所述预热池温度调整装置对所述预热池的温度的调整包括：
    随着所述计数池进入休眠的时间变长而逐渐调低所述预热池的温度。
  17. 根据权利要求16所述的血液细胞分析仪，其特征在于，所述预热池温度调整装置对所述预热池的温度的调整包括：
    在所述计数池刚进入休眠时，将所述预热池的温度设置为第一温度；
    在所述计数池进入休眠的时间段达到第一时间段时，将所述预热池的温度设置为第二温度，所述第二温度低于所述第一温度；以及
    在所述计数池进入休眠的时间段达到第二时间段时，将所述预热池的温度设置为第三温度，所述第二时间段大于所述第一时间段，所述第三温度低于所述第二温度。
  18. 根据权利要求10-13和15-17中的任一项所述的血液细胞分析仪，其特征在于，所述血液细胞分析仪还包括：
    休眠时间计时装置，用于在所述计数池进入休眠之后，对所述计数池进入休眠的时间计时；并且
    所述排气泡装置还用于：在所述计数池进入休眠之后，基于所述休眠时间计时装置的计时每隔预定时间间隔就将所述预热池中的气泡排出。
  19. 根据权利要求10-13和15-17中的任一项所述的血液细胞分析仪，其特征在于，所述排气泡装置还用于：
    在所述计数池每次进行白细胞计数和分类之前，均将所述预热池中的气泡排出。
PCT/CN2019/104497 2019-09-05 2019-09-05 血液细胞分析仪的分析方法和血液细胞分析仪 WO2021042319A1 (zh)

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