WO2021023836A1 - Dosage amélioré de détection d'auto-anticorps - Google Patents

Dosage amélioré de détection d'auto-anticorps Download PDF

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Publication number
WO2021023836A1
WO2021023836A1 PCT/EP2020/072176 EP2020072176W WO2021023836A1 WO 2021023836 A1 WO2021023836 A1 WO 2021023836A1 EP 2020072176 W EP2020072176 W EP 2020072176W WO 2021023836 A1 WO2021023836 A1 WO 2021023836A1
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WO
WIPO (PCT)
Prior art keywords
receptor
phospholipase
bead
secondary antibody
sample
Prior art date
Application number
PCT/EP2020/072176
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English (en)
Inventor
Cornelia DÄHNRICH
Sarah KONITZER
Original Assignee
Euroimmun Medizinische Labordiagnostika Ag
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Publication date
Application filed by Euroimmun Medizinische Labordiagnostika Ag filed Critical Euroimmun Medizinische Labordiagnostika Ag
Priority to EP20749923.7A priority Critical patent/EP4010704A1/fr
Publication of WO2021023836A1 publication Critical patent/WO2021023836A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/34—Genitourinary disorders
    • G01N2800/347—Renal failures; Glomerular diseases; Tubulointerstitial diseases, e.g. nephritic syndrome, glomerulonephritis; Renovascular diseases, e.g. renal artery occlusion, nephropathy

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Rheumatology (AREA)
  • Rehabilitation Therapy (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne l'utilisation d'un anticorps secondaire comprenant un marqueur chimio-luminescent pour augmenter la fiabilité diagnostique d'un dosage comprenant l'étape de détection d'un auto-anticorps contre le récepteur de la phospholipase-A2, et d'un kit A comprenant une perle comprenant un anticorps secondaire qui reconnaît les anticorps de la classe IgG et/ou le récepteur de la phospholipase-A2 ou une variante de celui-ci, l'anticorps et/ou le récepteur de la phospholipase-A2 comprenant un marqueur chimio-luminescent, et un calibreur qui définit une limite de détection inférieure de la chimioluminescence d'au moins 4, de préférence une limite inférieure d'au moins 3, plus préférablement une limite inférieure d'au moins 2 CU/ml, ainsi qu'un ou plusieurs, de préférence la totalité des éléments du groupe comprenant une ou plusieurs solutions de calibreur, un tampon de dilution d'échantillon, une solution de lavage, un témoin positif, un témoin négatif et une solution de déclenchement de la chimio-luminescence.
PCT/EP2020/072176 2019-08-06 2020-08-06 Dosage amélioré de détection d'auto-anticorps WO2021023836A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP20749923.7A EP4010704A1 (fr) 2019-08-06 2020-08-06 Dosage amélioré de détection d'auto-anticorps

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP19190245 2019-08-06
EP19000365.7 2019-08-06
EP19190245.1 2019-08-06
EP19000365 2019-08-06

Publications (1)

Publication Number Publication Date
WO2021023836A1 true WO2021023836A1 (fr) 2021-02-11

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PCT/EP2020/072176 WO2021023836A1 (fr) 2019-08-06 2020-08-06 Dosage amélioré de détection d'auto-anticorps

Country Status (2)

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EP (1) EP4010704A1 (fr)
WO (1) WO2021023836A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117434276A (zh) * 2023-12-12 2024-01-23 南京羿检医学科技有限公司 一种thsd7a抗体检测试剂盒及其用途

Citations (6)

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EP2313778A1 (fr) * 2008-07-18 2011-04-27 Boston Medical Center Corporation Diagnostics pour une néphropathie membraneuse
WO2012028167A2 (fr) 2010-05-20 2012-03-08 Innohyphen Bv Chimioluminescence d'ester d'acridinium lors du déclenchement réducteur
US20170137490A1 (en) 2014-06-06 2017-05-18 The University Of Manchester Peptides, and methods and apparatus utilising same
US10107810B2 (en) 2014-07-24 2018-10-23 Euroimmun Medizinische Labordiagnostika Ag Methods and kits for monitoring membranous nephropathy
CN109459572A (zh) * 2018-12-21 2019-03-12 江南大学 一种原发性膜性肾病诊断试剂盒
US20190183969A1 (en) 2016-08-12 2019-06-20 Immunowork, Llc Diagnosis, prevention, and/or treatment of autoimmune diseases

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2313778A1 (fr) * 2008-07-18 2011-04-27 Boston Medical Center Corporation Diagnostics pour une néphropathie membraneuse
US20130280738A1 (en) 2008-07-18 2013-10-24 Le Centre National De La Recherche Scientifique Diagnostics for membranous nephropathy
WO2012028167A2 (fr) 2010-05-20 2012-03-08 Innohyphen Bv Chimioluminescence d'ester d'acridinium lors du déclenchement réducteur
US20170137490A1 (en) 2014-06-06 2017-05-18 The University Of Manchester Peptides, and methods and apparatus utilising same
US10107810B2 (en) 2014-07-24 2018-10-23 Euroimmun Medizinische Labordiagnostika Ag Methods and kits for monitoring membranous nephropathy
US20190183969A1 (en) 2016-08-12 2019-06-20 Immunowork, Llc Diagnosis, prevention, and/or treatment of autoimmune diseases
CN109459572A (zh) * 2018-12-21 2019-03-12 江南大学 一种原发性膜性肾病诊断试剂盒

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BECK, L.BONEGIO, R. G.LAMBEAU, G.BECK, D. M.POWELL, D. W.CUMMINS, T. D.KLEIN J. B.SALANT, D. J., N. ENGL. J. MED., vol. 361, no. 1, 2009, pages 11 - 21
BEHNERT, A.FRITZLER, M. J.TENG, B.ZHANG, M.BOLLIG, F.HALLER, H.SKOBERNE, A.MAHLER, M.SCHIFFER, M., PLOS, vol. 8, no. 4, 2013, pages e61669
BROWN T. A.: "Gene Cloning - an introduction", 1986, CHAPMAN & HALL
BURGESS, R. R.DEUTSCHER, M. P., GUIDE TO PROTEIN PURIFICATION, 2009
CORNELIA DÄHNRICH ET AL: "Development of a standardized chemiluminescence immunoassay for the detection of autoantibodies against human M-type phospholipase A2 receptor in primary membranous nephropathy", KIDNEY INTERNATIONAL REPORTS, 1 November 2019 (2019-11-01), XP055647818, ISSN: 2468-0249, DOI: 10.1016/j.ekir.2019.11.008 *
FRESQUET, M.JOWITT, T. A.GUMMADOVA, J., J. AM. NEPHROL., vol. 26, 2015, pages 302 - 313
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LARKIN, M. A.BLACKSHIELDS, G.BROWN, N. P.CHENNA, R.MCGETTIGAN, P. A.MCWILLIAM, H.VALENTIN, F.WALLACE, I. M.WILM, A.LOPEZ, R.: "Clustal W and Clustal X version 2.0", BIOINFORMATICS, vol. 23, 2007, pages 2947 - 2948
QIUHUA ZHANG ET AL: "Ultrasensitive Quantitation of Anti-Phospholipase A2 Receptor Antibody as A Diagnostic and Prognostic Indicator of Idiopathic Membranous Nephropathy", SCIENTIFIC REPORTS, vol. 7, no. 1, 21 September 2017 (2017-09-21), XP055647817, DOI: 10.1038/s41598-017-12014-1 *
SAMBROOK, J.FRITSCH, E. F.MANIATIS, T.: "Molecular Cloning", 1989, CSH
TIMMERMANS, S. ADAMOISEAUX, J. G.HEERINGS-REWIKEL, P. T.AYLAON, R.BECK, L. H.SCHLUMBERGER, W.SALANT, D. J.VAN PASSEN, PTERVAERT, J, AM. J. CLIN. PATHOL., vol. 142, 2014, pages 29 - 34
WEEKS, I.BEHESHTI, I.MCCAPRA, F.CAMPBELL, A. K.WOODHEAD, J. S.: "Acridinium esters as high specific activity labels in immunoassay", CLIN CHEM, vol. 29, 1983, pages 1474 - 1479, XP002097142

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117434276A (zh) * 2023-12-12 2024-01-23 南京羿检医学科技有限公司 一种thsd7a抗体检测试剂盒及其用途

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Publication number Publication date
EP4010704A1 (fr) 2022-06-15

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