WO2020256375A2 - Seringue d'électroporation et système d'injection par électroporation l'utilisant - Google Patents

Seringue d'électroporation et système d'injection par électroporation l'utilisant Download PDF

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Publication number
WO2020256375A2
WO2020256375A2 PCT/KR2020/007765 KR2020007765W WO2020256375A2 WO 2020256375 A2 WO2020256375 A2 WO 2020256375A2 KR 2020007765 W KR2020007765 W KR 2020007765W WO 2020256375 A2 WO2020256375 A2 WO 2020256375A2
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WO
WIPO (PCT)
Prior art keywords
electroporation
terminal
coated
syringe
animal
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Application number
PCT/KR2020/007765
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English (en)
Korean (ko)
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WO2020256375A3 (fr
Inventor
김균
김은진
Original Assignee
(주)플럼라인생명과학
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from KR1020200067644A external-priority patent/KR102470989B1/ko
Application filed by (주)플럼라인생명과학 filed Critical (주)플럼라인생명과학
Publication of WO2020256375A2 publication Critical patent/WO2020256375A2/fr
Publication of WO2020256375A3 publication Critical patent/WO2020256375A3/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D1/00—Surgical instruments for veterinary use
    • A61D1/02—Trocars or cannulas for teats; Vaccination appliances

Definitions

  • the present invention relates to a syringe for electroporation and an injection system for electroporation using the same.
  • a syringe for electroporation in order to safely and efficiently deliver a drug such as a DNA vaccine material into cells, an electric stimulation configured to be intensively delivered to a local area
  • a perforation syringe and an electroporation injection system using the same.
  • Electroporation is a technology that generates electric current and delivers DNA (DeoxyriboNucleic Acid) plasmid into cells. This technique uses electric pulses (square-wave) to create a temporary passage through the cell membrane, which injects the DNA plasmid into the cytoplasm and the cell nucleus. This is the most efficient and safe way to deliver DNA vaccine materials into cells.
  • a DNA vaccine is a substance composed of DNA that contains information about a therapeutic substance and a plasmid (vector) to deliver it.
  • a DNA vaccine By administering such a DNA vaccine into the body of an animal through electroporation, the efficiency of intracellular delivery of genes is improved, and by optimally designed according to the animal species, CD4 + , CD8 + T cell responses (cellular immunity) and antibody formation It has a great effect on the formation of immune capacity such as reaction (humoral immunity).
  • the technical problem to be solved by the present invention is to provide a syringe for electroporation and an injection system for electroporation using the same, and a syringe for electroporation configured to simultaneously proceed with injection of a chemical solution such as a DNA vaccine and generation of an electric pulse through one syringe.
  • Another technical problem to be solved by the present invention is an electroporation syringe configured to minimize skin damage other than a specific area while simultaneously applying electric pulse stimulation to a desired area (especially a specific location in a deep skin area), and an electric cloth using the same. It is to provide a common injection system.
  • An electroporation syringe for use in an animal and for delivering a DNA vaccine material into cells, comprising: a body into which a chemical liquid entering the body of the animal is introduced; A coupler formed on the body; An injection needle connected to the body through the coupler and receiving a chemical solution from the body and injected into the body of the animal; And a connector formed on one of the coupler and the injection needle and connected to at least one of the (+) terminal and the (-) terminal of the electric generator.
  • a chemical solution discharge hole composed of at least one hole; is further provided at a side circumference of the injection needle inserted into the body of the animal.
  • a first coating part coated with a non-toxic insulating material is formed on the outer circumferential surface of the injection needle, and at least one first non-coated part not coated with the non-toxic insulating material is formed on the first coating part inserted into the body of the animal. It is formed in the position, characterized in that the first non-coated portion is characterized in that further provided with a chemical liquid discharge hole consisting of at least one hole.
  • a terminal forming portion coupled to the outer circumferential surface of the first coating portion and formed of a conductive material to which the connector is coupled; And a second coating part coated with a non-toxic insulating material on the outer circumferential surface of the terminal forming part, and a second non-coating part not coated with the non-toxic insulating material on the second coating part inserted into the body of the animal It is characterized in that it is formed in at least one place.
  • the coupler is formed of two, so that the couplers are insulated from each other, one coupler is connected to the (+) terminal of the electric generator, and the other coupler is connected to the (-) terminal of the electric generator, It characterized in that the injection needle is coupled to each of the couplers.
  • the two injection needles inserted into the body of the animal face each other, a chemical solution discharge hole consisting of at least one hole; characterized in that further provided.
  • a first coating part coated with a non-toxic insulating material is formed on the outer circumferential surface of each of the injection needles, and the first non-coated part not coated with the non-toxic insulating material is formed on the first coating part inserted into the body of the animal. It is formed in at least one place, characterized in that a chemical liquid discharge hole formed of at least one hole is further provided in a portion facing each other in the first uncoated portion.
  • the electric generator A body through which a chemical liquid entering the body of an animal flows into the body, a coupler formed in the body, an injection needle connected to the body through the coupler and receiving the chemical liquid from the body and injecting it into the body of the animal, the coupler and the An electroporation syringe formed on any one of the injection needles and including a connector connected to any one of the (+) terminal and the (-) terminal of the electric generator; And at least one attachment pad connected to the other one of the (+) terminal and the (-) terminal of the electric generator and attached to the skin of the animal, wherein the electroporation syringe comprises: of the injection needle.
  • a first coating portion coated with a non-toxic insulating material is formed on the outer circumferential surface, and a first non-coated portion not coated with the non-toxic insulating material is formed on at least one portion of the first coating portion inserted into the body of the animal. do.
  • the electroporation syringe is characterized in that it is further provided with a chemical solution discharge hole composed of at least one hole at the circumference of the side of the injection needle inserted into the body of the animal.
  • it is characterized in that it further comprises a polarity controller for transmitting by changing the (+) polarity and (-) polarity of the electric generator.
  • the electric generator And a body through which a chemical liquid entering the body of an animal is introduced, a coupler formed in the body, an injection needle connected to the body through the coupler and receiving the chemical liquid from the body and injecting it into the body of the animal, and the coupler.
  • an electroporation syringe formed in any one of the injection needles and including a connector connected to at least one of the (+) terminal and the (-) terminal of the electric generator, wherein the electroporation syringe ,
  • a first coating part coated with a non-toxic insulating material is formed on the outer circumferential surface of the injection needle connected to any one of the (+) terminal and the (-) terminal of the electric generator, and is inserted into the body of the animal.
  • the first non-coated part which is not coated with the non-toxic insulating material, is formed on at least one part of the first coating part, is coupled to the outer circumferential surface of the first coating part, and is made of a conductive material, so that the (+) terminal of the electric generator and the A terminal forming part to which the connector is coupled so as to be connected to the other one of the (-) terminals; And a second coating part coated with a non-toxic insulating material on the outer circumferential surface of the terminal forming part, wherein the second coating part inserted into the body of the animal has a second non-coated part not coated with the non-toxic insulating material It is characterized in that it is formed in at least one place.
  • it characterized in that it further comprises at least one attachment pad connected to any one of the (+) terminal and the (-) terminal of the electric generator and attached to the skin of the animal.
  • it is characterized in that it further comprises a polarity controller for transmitting by changing the (+) polarity and (-) polarity of the electric generator.
  • the dose of the DNA vaccine can be increased and the range of administration can be expanded, thereby enhancing the efficiency of intracellular DNA vaccine delivery.
  • one of the (+) and (-) terminals of the electric generator is formed on the needle inserted into the animal body, and the other terminal is connected to the attachment pad that attaches (or adheres to) the animal's skin.
  • a chemical solution such as a DNA vaccine or the like
  • electroporation syringe it is possible to inject a chemical solution such as a DNA vaccine or the like through an electroporation syringe and simultaneously apply an electric pulse from the skin of an animal to a terminal portion formed on the needle.
  • the electric pulse can be accurately transmitted to the desired site, so that the DNA vaccine material can be efficiently and safely delivered into the cell.
  • electrical stimulation can be applied at the same time with injection needles at a plurality of positions, thereby further increasing the effect of electroporation.
  • both the (+) and (-) terminals of the electric generator are configured with one needle, the equipment is further simplified, so product miniaturization is possible, production cost can be reduced, and convenience of use can be improved. .
  • stimulation applied to an animal can be minimized compared to a case in which a syringe for injecting a chemical solution such as a DNA vaccine material and a syringe for providing electrical stimulation are simultaneously used as in the prior art.
  • FIG. 1 is a conceptual diagram of a configuration according to an embodiment of an electroporation syringe according to the present invention
  • Figure 2 is a conceptual diagram of the configuration according to another embodiment of the electroporation syringe according to the present invention.
  • FIG. 3 is a conceptual diagram showing an electrical stimulation pattern according to the configuration of FIG. 2;
  • FIG. 4 is a conceptual diagram showing another embodiment of the present inventors electroporation syringe and an injection system for electroporation using the same,
  • FIG. 5 is a conceptual diagram illustrating a state in which a plurality of first non-coated portions and an attachment pad are respectively configured in the configuration of FIG. 4;
  • Figure 6 is a schematic diagram of a configuration according to another embodiment of the present inventors electroporation injector and an electroporation injection system using the same,
  • FIG. 7 is a conceptual diagram showing a state in which a plurality of (+) terminals are configured in the configuration of FIG. 6;
  • first, second, A, B, (a), and (b) may be used. These terms are only used to distinguish the component from other components, and the nature, order, or order of the component is not limited by the term.
  • FIG. 1 is a conceptual diagram of the configuration according to an embodiment of an electroporation syringe 20 according to the present invention
  • Figure 2 is a conceptual diagram of the configuration according to another embodiment of the electroporation syringe 20 according to the present invention
  • Figure 3 Is a conceptual diagram showing an electrical stimulation pattern according to the configuration of FIG. 2.
  • the electroporation syringe 20 includes a body 21, a coupler 22, an injection needle 23, and a connector 24.
  • the body 21 is a part into which the chemical liquid entering the body of the animal is introduced.
  • the chemical solution may be injected into the body through the rear or side surfaces of the body.
  • the electroporation syringe 20 may be in the form of a gun, for example. Since the shape and operation method of the gun-type syringe are already known techniques, detailed descriptions thereof will be omitted.
  • a coupler 22 is connected to the lower portion of the body 21.
  • the coupler 22 is formed on the body 21 and is a portion to which the injection needle 23 is detached.
  • the coupling type of the coupler 22 and the injection needle 23 has a known male and female coupling structure.
  • a helical hole (not shown) may be provided in the coupler 22, and a protrusion (not shown) may be provided at the upper circumference of the injection needle 23 to correspond to the helical hole. . That is, the injection needle 23 is rotated forward to fasten the protrusion to the helical hole, and the injection needle 23 is rotated in reverse to separate from the helical hole.
  • the coupler 22 and the injection needle 23 are sealedly coupled to each other so that the chemical solution does not leak to the fastened portion.
  • FIG. 1 shows a structure in which two injection needles 23 are coupled. That is, under the coupler 22, two coupling portions (not shown in the drawings) for coupling the injection needle 23 are formed. The coupling parts are spaced apart by a certain distance. An injection needle 23 is fastened to each coupling part.
  • the injection needle 23 is connected to the body 21 through a coupler 22.
  • the coupler 22 receives the drug solution from the body 21, and the delivered drug solution is injected into the body of the animal through the injection needle 23.
  • the injection needle 23 may have a form in which a lower end is opened and a chemical solution flows through the open lower end.
  • chemical solution discharge holes 23-5 may be formed on the circumferential surfaces of the two injection needles 23 facing each other.
  • the chemical liquid discharge hole 23-5 is composed of at least one hole.
  • the chemical liquid discharge hole 23-5 may be formed in at least one position. .
  • the chemical solution discharge holes 23-5 may be formed outside the two injection needles 23, respectively, not facing each other.
  • a connector 24 connected to at least one of the (+) terminal and the (-) terminal of the electric generator 10 is formed on one of the coupler 22 and the injection needle 23.
  • the connector 24 may be exposed to the outside or may be provided inside.
  • 1 conceptually illustrates a state in which the connector 24 is formed inside the coupler 22.
  • the connector 24 Through the connector 24, one of the two needles 23 is connected to the (+) terminal of the electric generator 10, and the other injection needle 23 is connected to the electric generator ( It is connected to the (-) terminal of 10).
  • it is a structure in which a chemical solution is delivered into cells located between the two injection needles 23 while applying electrical stimulation through the two injection needles 23.
  • FIG. 2 The configuration of FIG. 2 is the same as that of FIG. 1, but has a structure coated with an insulating material on the outer peripheral surface of the injection needle 23. That is, as shown in FIG. 2, a first coating portion 23-1 coated with a non-toxic insulating material is formed on the outer peripheral surface of the injection needle 23. In addition, a first non-coated part 23-2 that is not coated with a non-conductive insulating material is formed on the first coated part 23-1 inserted into the body of the animal. 2 shows that the first uncoated portion 23-2 is formed under the injection needle 23. The first uncoated portion 23-2 may be formed at a plurality of positions. The first uncoated part 23-2 is formed with a chemical liquid discharge hole 23-5 composed of at least one hole.
  • the chemical solution discharge hole 23-5 formed in the first uncoated part 23-2 may also be formed in at least one place of the first uncoated part 23-2, that is, at a plurality of locations, although not shown. .
  • the chemical liquid discharge hole 23-5 is composed of at least one hole and may be formed in a plurality of positions without a separate description.
  • FIG. 4 is a conceptual diagram showing another embodiment of an electroporation syringe 20 according to the present invention and an injection system for electroporation using the same
  • FIG. 5 is a first non-coated part 23-2 in the configuration of FIG. It is a conceptual diagram of a state in which a plurality of attachment pads 30 are respectively configured.
  • an electroporation syringe 20 is composed of a body 21, a coupler 22, an injection needle 23 and a connector 24, and an injection needle ( 23) consists of one.
  • an injection needle ( 23) consists of one.
  • FIGS. 1 and 2 For a basic configuration state, the description of FIGS. 1 and 2 may be referred to.
  • the injection needle 23 is composed of a conductor that conducts electricity.
  • a connector 24 is formed on the upper portion of the injection needle 23 connected to the body 21.
  • the connection structure between the connector 24 and the injection needle 23 can be applied to various known connection methods, and the injection needle 23 and the connector 24 are electrically connected.
  • One of the (+) terminal and the (-) terminal of the electric generator 10 may be connected to the connector 24.
  • the connector 24 may be formed on the coupler 22 instead of the injection needle 23.
  • the shape of the connector 24 is shown only conceptually, and the shape and configuration position thereof may be variously implemented at the level of those skilled in the art.
  • the entire circumferential surface of the injection needle 23 except for the first non-coated part 23-2 is coated with a non-toxic insulating material to form the first coated part 23-1.
  • the first uncoated portion 23-2 may be formed at one lower portion of the injection needle 23, or may be formed at the middle or upper portion of the injection needle 23 although not shown.
  • it may be formed at least in a plurality of positions (the middle part and the lower part), and the formation position is a position to deliver a drug solution (hereinafter referred to as'DNA vaccine') such as a DNA vaccine material. It can be configured in correspondence.
  • the length of the injection needle 23 can be standardized and manufactured differently, and the first uncoated portion 23-2 can also be standardized and mass-produced. Therefore, according to the animal species or the site where the DNA vaccine is to be injected, the standardized injection needle (23) is replaced and used, or the electroporation syringe (20) and the injection needle (23) are integrally standardized and mass produced.
  • the common syringe 20 itself may be replaced and used.
  • the injection needle 23 When the DNA vaccine is injected, the injection needle 23 is inserted into the body of the animal, and the DNA vaccine of the body 21 is injected into the body of the animal through the injection needle 23.
  • the first non-coated part 23-2 is configured to be located in the body of the animal when the injection needle 23 is inserted into the body of the animal.
  • the electroporation injection system using such an electroporation syringe 20 includes an electric generator 10, an electroporation syringe 20, and an attachment pad 30.
  • the electricity generator 10 generates a constant current or a constant voltage, and (+) and (-) terminals are formed. That is, 220 volt commercial power is applied and a constant current or constant voltage is output to generate an electric pulse (square-wave, square wave).
  • a constant current or constant voltage it is preferable to use a constant current without a change in the electric pulse, since the loss of the electric pulse may cause damage to the cell tissue as well as the delivery efficiency of the intracellular DNA vaccine may be reduced.
  • the electroporation syringe 20 is for injecting a DNA (DeoxyriboNucleic Acid) vaccine into the body of an animal.
  • a DNA DeoxyriboNucleic Acid
  • the injection needle 23 and the body 21 to which the DNA vaccine is injected are used with a medical hose, etc. It includes a connected type of ring gel, etc.
  • it will include all known medical items in the form of a connection with an injection needle 23 capable of injecting a DNA vaccine.
  • the attachment pad 30 is connected to the other terminal of the (+) terminal and the (-) terminal of the electric generator 10.
  • the (+) terminal of the electric generator 10 is connected to the attachment pad 30. Is connected through a cable 40.
  • the attachment pad 30 may be configured as one as shown in FIG. 4 or may be configured as a plurality as shown in FIG. 5.
  • the (+) terminal of the electric generator 10 is connected to each of the attachment pads 30 through respective cables 40.
  • the attachment pad 30 may also be attached to the opposite side of the skin 1 on which the 23 is inserted, so that electric stimulation may be applied from all directions.
  • the surface of the attachment pad 30 attached to the animal's skin 1 is made of a conductive material and has a structure that is connected to the (+) terminal of the electric generator 10.
  • a conductive adhesive for attaching to the skin 1 of an animal may be formed on the surface of the attachment pad 30.
  • a chemical solution discharge hole 23-5 communicating with the inside of the injection needle 23 may be formed in the first uncoated part 23-2.
  • the chemical liquid discharge holes 23-5 may be formed on both sides of the circumference of the first uncoated part 23-2, or may be formed in all directions. Since the DNA vaccine is discharged from a plurality of locations through the drug solution discharge hole 23-5 formed as described above, it is possible to induce the DNA vaccine to be well delivered into the cell with electric pulse stimulation.
  • FIG. 6 is a conceptual diagram showing a configuration according to another embodiment of an electroporation syringe 20 and an electroporation injection system using the same, and FIG. 7 shows a state in which a plurality of (+) terminals are configured in the configuration of FIG. It is a conceptual diagram.
  • the electroporation syringe 20 has a structure in which both terminals (+) and (-) of the electric generator 10 are connected to the injection needle 23.
  • the first coated part 23, the first uncoated part 23-2, the two terminal forming parts 24, the second coated part 23-3, and the second non-coated part on the injection needle 23 It includes part (23-4).
  • a connector 24 connected to any one of the positive and negative terminals of the electric generator 10 is electrically connected to the injection needle 23 of the electroporation syringe 20.
  • the first coating part 23-1 is coated with a non-toxic insulating material surrounding the outer peripheral surface of the injection needle 23 except for the connection part of the cable 40 of the connector 24.
  • at least one first non-coated portion 23-2 which is not coated with a non-toxic insulating material, is present at a site inserted into the body of the animal. That is, as in the configuration of FIG. 5, a plurality of first uncoated portions 23-2 may be formed.
  • the first uncoated portion 23-2 has a chemical solution discharge hole 23-5 communicating with the inside of the injection needle 23.
  • the terminal forming part 25 is formed on the outer circumferential surface of the first coating part 23-1.
  • the connector 24 is also connected to the terminal forming unit 25, and the terminal forming unit 25 through the connector 24 is connected to the other terminal among the (+) and (-) terminals of the electric generator 10. Connected. For example, if the (-) terminal is connected to the connector 24 connected to the injection needle 23, the (+) terminal is connected to the connector 24 connected to the terminal forming part 25.
  • the terminal forming part 25 is formed on a part of the circumferential surface of the first coated part 23-1, and does not interfere with the first uncoated part 23-2.
  • the terminal forming part 25 may be formed of a metal material surrounding the first coating part 23-1 and may be connected to the connector 24 or may be formed of a wire electrically connected to the connector 24.
  • the connector 24 formed on the injection needle 23 may be configured inside the coupler 22. Meanwhile, the connector 24 connected to the injection needle 23 and the connector 24 connected to the terminal forming part 25 are not electrically connected to each other.
  • the (+) terminal of the electric generator 10 may be connected to the connector 24 connected to the terminal forming part 25 as well as an attachment pad 30 additionally.
  • the attachment pad 30 may be connected to the opposite side of the skin 1 into which the injection needle 23 is inserted, as shown by the double-dashed line in FIG. 5. That is, it is possible to increase the treatment effect and reduce tissue damage by configuring to apply electrical stimulation from a plurality of locations to one place.
  • the second coating portion 23-3 is coated with a non-toxic insulating material on the outer peripheral surface of the terminal forming portion 25.
  • a second non-coated part 23-4 which is not coated with a non-toxic insulating material, is present. Meanwhile, in the drawing, a state in which the second uncoated portion 23-4 is configured as one under the terminal forming portion 25 is shown.
  • a chemical liquid discharge hole composed of at least one hole may be formed in the first uncoated part 23-2.
  • the non-toxic insulating material applied to the present invention is preferably made of an insulating material harmless to animals such as non-toxic silicone.
  • the electric generator 10 applied to the present invention can be used by connecting with a polarity controller (not shown) to change the (+) polarity and the (-) polarity. This is a known technique, so a detailed description thereof will be omitted. do.
  • DNA vaccine is injected into the body 21.
  • a cable 40 is connected between the connector 24 formed on the injection needle 23 and the (-) terminal of the electric generator 10.
  • the injection needle 23 of the electroporation syringe 20 is inserted into the body of the animal.
  • the attachment pad 30 forms a (+) pole
  • the first uncoated portion 23-2 of the injection needle 23 forms a (-) pole.
  • the animal's skin (1) located between the (+) and (-) poles, acts as a kind of resistance and forms a closed loop as a whole. Accordingly, as an electric pulse is applied from the attachment pad 30 to the lower uncoated portion 23-2 of the injection needle 23, a passage is temporarily formed in the peripheral cell membrane of the injection needle 23.
  • the DNA vaccine introduced into the body of the animal from the body 21 through the injection needle 23 penetrates into the cytoplasm and the cell nucleus through the passage temporarily formed as described above.
  • DNA vaccine is injected into the body 21.
  • a cable 40 is connected between the connector 24 formed on the terminal forming part 24 and the (+) terminal of the electric generator 10.
  • the medical syringe 20 is inserted into the animal body.
  • the terminal forming part 25 forms a (+) pole
  • the lower first uncoated part 23-2 of the injection needle 23 forms a (-) pole.
  • the animal skin (1) located between the (+) and (-) poles acts as a kind of resistance and forms a closed loop as a whole.
  • the injection needle (23) A passage is temporarily formed in the surrounding cell membrane.
  • the DNA vaccine introduced into the body of the animal from the body 21 through the injection needle 23 penetrates into the cytoplasm and the cell nucleus through the passage temporarily formed as described above.
  • the DNA vaccine injection and electric pulse stimulation can be performed through one injection needle 23, stimulation due to insertion of the injection needle 23 in the animal can be reduced.
  • the overall configuration of the electroporation syringe 20 and the injection system using the same is simplified, the volume can be reduced and convenience of use can be improved.
  • the formation position of the first non-coating part 23-2 to correspond to the position to which the DNA vaccine is to be delivered, the therapeutic effect can be improved and the DNA vaccine delivery efficiency can be further improved.

Abstract

La présente invention concerne une seringue d'électroporation et un système d'injection par électroporation l'utilisant, la seringue d'électroporation étant configurée pour administrer de manière intensive une stimulation électrique à une zone locale afin de délivrer de manière sûre et efficace une solution de médicament telle qu'un matériau de vaccin à ADN dans des cellules. Plus spécifiquement, la présente invention concerne une seringue d'électroporation et un système d'injection par électroporation l'utilisant, la seringue d'électroporation comprenant: un corps dans lequel s'écoule une solution de médicament entrant dans le corps d'un animal; un coupleur formé sur le corps; une aiguille d'injection reliée au corps par l'intermédiaire du coupleur, et recevant la solution de médicament du corps et l'injectant dans le corps de l'animal; et un connecteur formé sur l'un quelconque du coupleur ou de l'aiguille d'injection et relié à au moins une borne des deux bornes (+) et (-) d'un générateur électrique.
PCT/KR2020/007765 2019-06-18 2020-06-16 Seringue d'électroporation et système d'injection par électroporation l'utilisant WO2020256375A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2019-0072268 2019-06-18
KR20190072268 2019-06-18
KR1020200067644A KR102470989B1 (ko) 2019-06-18 2020-06-04 전기천공용 주사기 및 이를 이용한 전기천공용 주사 시스템
KR10-2020-0067644 2020-06-04

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WO2020256375A2 true WO2020256375A2 (fr) 2020-12-24
WO2020256375A3 WO2020256375A3 (fr) 2021-04-15

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Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8659598A (en) * 1997-07-22 1999-02-16 Emed Corporation Iontophoretic delivery of an agent into cardiac tissue
US6678556B1 (en) * 1998-07-13 2004-01-13 Genetronics, Inc. Electrical field therapy with reduced histopathological change in muscle
IL144948A0 (en) * 1999-02-18 2002-06-30 Biovalve Technologies Inc Electroactive pore
US7328064B2 (en) * 2002-07-04 2008-02-05 Inovio As Electroporation device and injection apparatus
CA2839196A1 (fr) * 2011-06-15 2012-12-20 Chrontech Pharma Ab Aiguille et dispositif d'injection

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