WO2020256375A2 - Electroporation syringe and electroporation injection system using same - Google Patents

Electroporation syringe and electroporation injection system using same Download PDF

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Publication number
WO2020256375A2
WO2020256375A2 PCT/KR2020/007765 KR2020007765W WO2020256375A2 WO 2020256375 A2 WO2020256375 A2 WO 2020256375A2 KR 2020007765 W KR2020007765 W KR 2020007765W WO 2020256375 A2 WO2020256375 A2 WO 2020256375A2
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WO
WIPO (PCT)
Prior art keywords
electroporation
terminal
coated
syringe
animal
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PCT/KR2020/007765
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French (fr)
Korean (ko)
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WO2020256375A3 (en
Inventor
김균
김은진
Original Assignee
(주)플럼라인생명과학
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Priority claimed from KR1020200067644A external-priority patent/KR102470989B1/en
Application filed by (주)플럼라인생명과학 filed Critical (주)플럼라인생명과학
Publication of WO2020256375A2 publication Critical patent/WO2020256375A2/en
Publication of WO2020256375A3 publication Critical patent/WO2020256375A3/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D1/00—Surgical instruments for veterinary use
    • A61D1/02—Trocars or cannulas for teats; Vaccination appliances

Definitions

  • the present invention relates to a syringe for electroporation and an injection system for electroporation using the same.
  • a syringe for electroporation in order to safely and efficiently deliver a drug such as a DNA vaccine material into cells, an electric stimulation configured to be intensively delivered to a local area
  • a perforation syringe and an electroporation injection system using the same.
  • Electroporation is a technology that generates electric current and delivers DNA (DeoxyriboNucleic Acid) plasmid into cells. This technique uses electric pulses (square-wave) to create a temporary passage through the cell membrane, which injects the DNA plasmid into the cytoplasm and the cell nucleus. This is the most efficient and safe way to deliver DNA vaccine materials into cells.
  • a DNA vaccine is a substance composed of DNA that contains information about a therapeutic substance and a plasmid (vector) to deliver it.
  • a DNA vaccine By administering such a DNA vaccine into the body of an animal through electroporation, the efficiency of intracellular delivery of genes is improved, and by optimally designed according to the animal species, CD4 + , CD8 + T cell responses (cellular immunity) and antibody formation It has a great effect on the formation of immune capacity such as reaction (humoral immunity).
  • the technical problem to be solved by the present invention is to provide a syringe for electroporation and an injection system for electroporation using the same, and a syringe for electroporation configured to simultaneously proceed with injection of a chemical solution such as a DNA vaccine and generation of an electric pulse through one syringe.
  • Another technical problem to be solved by the present invention is an electroporation syringe configured to minimize skin damage other than a specific area while simultaneously applying electric pulse stimulation to a desired area (especially a specific location in a deep skin area), and an electric cloth using the same. It is to provide a common injection system.
  • An electroporation syringe for use in an animal and for delivering a DNA vaccine material into cells, comprising: a body into which a chemical liquid entering the body of the animal is introduced; A coupler formed on the body; An injection needle connected to the body through the coupler and receiving a chemical solution from the body and injected into the body of the animal; And a connector formed on one of the coupler and the injection needle and connected to at least one of the (+) terminal and the (-) terminal of the electric generator.
  • a chemical solution discharge hole composed of at least one hole; is further provided at a side circumference of the injection needle inserted into the body of the animal.
  • a first coating part coated with a non-toxic insulating material is formed on the outer circumferential surface of the injection needle, and at least one first non-coated part not coated with the non-toxic insulating material is formed on the first coating part inserted into the body of the animal. It is formed in the position, characterized in that the first non-coated portion is characterized in that further provided with a chemical liquid discharge hole consisting of at least one hole.
  • a terminal forming portion coupled to the outer circumferential surface of the first coating portion and formed of a conductive material to which the connector is coupled; And a second coating part coated with a non-toxic insulating material on the outer circumferential surface of the terminal forming part, and a second non-coating part not coated with the non-toxic insulating material on the second coating part inserted into the body of the animal It is characterized in that it is formed in at least one place.
  • the coupler is formed of two, so that the couplers are insulated from each other, one coupler is connected to the (+) terminal of the electric generator, and the other coupler is connected to the (-) terminal of the electric generator, It characterized in that the injection needle is coupled to each of the couplers.
  • the two injection needles inserted into the body of the animal face each other, a chemical solution discharge hole consisting of at least one hole; characterized in that further provided.
  • a first coating part coated with a non-toxic insulating material is formed on the outer circumferential surface of each of the injection needles, and the first non-coated part not coated with the non-toxic insulating material is formed on the first coating part inserted into the body of the animal. It is formed in at least one place, characterized in that a chemical liquid discharge hole formed of at least one hole is further provided in a portion facing each other in the first uncoated portion.
  • the electric generator A body through which a chemical liquid entering the body of an animal flows into the body, a coupler formed in the body, an injection needle connected to the body through the coupler and receiving the chemical liquid from the body and injecting it into the body of the animal, the coupler and the An electroporation syringe formed on any one of the injection needles and including a connector connected to any one of the (+) terminal and the (-) terminal of the electric generator; And at least one attachment pad connected to the other one of the (+) terminal and the (-) terminal of the electric generator and attached to the skin of the animal, wherein the electroporation syringe comprises: of the injection needle.
  • a first coating portion coated with a non-toxic insulating material is formed on the outer circumferential surface, and a first non-coated portion not coated with the non-toxic insulating material is formed on at least one portion of the first coating portion inserted into the body of the animal. do.
  • the electroporation syringe is characterized in that it is further provided with a chemical solution discharge hole composed of at least one hole at the circumference of the side of the injection needle inserted into the body of the animal.
  • it is characterized in that it further comprises a polarity controller for transmitting by changing the (+) polarity and (-) polarity of the electric generator.
  • the electric generator And a body through which a chemical liquid entering the body of an animal is introduced, a coupler formed in the body, an injection needle connected to the body through the coupler and receiving the chemical liquid from the body and injecting it into the body of the animal, and the coupler.
  • an electroporation syringe formed in any one of the injection needles and including a connector connected to at least one of the (+) terminal and the (-) terminal of the electric generator, wherein the electroporation syringe ,
  • a first coating part coated with a non-toxic insulating material is formed on the outer circumferential surface of the injection needle connected to any one of the (+) terminal and the (-) terminal of the electric generator, and is inserted into the body of the animal.
  • the first non-coated part which is not coated with the non-toxic insulating material, is formed on at least one part of the first coating part, is coupled to the outer circumferential surface of the first coating part, and is made of a conductive material, so that the (+) terminal of the electric generator and the A terminal forming part to which the connector is coupled so as to be connected to the other one of the (-) terminals; And a second coating part coated with a non-toxic insulating material on the outer circumferential surface of the terminal forming part, wherein the second coating part inserted into the body of the animal has a second non-coated part not coated with the non-toxic insulating material It is characterized in that it is formed in at least one place.
  • it characterized in that it further comprises at least one attachment pad connected to any one of the (+) terminal and the (-) terminal of the electric generator and attached to the skin of the animal.
  • it is characterized in that it further comprises a polarity controller for transmitting by changing the (+) polarity and (-) polarity of the electric generator.
  • the dose of the DNA vaccine can be increased and the range of administration can be expanded, thereby enhancing the efficiency of intracellular DNA vaccine delivery.
  • one of the (+) and (-) terminals of the electric generator is formed on the needle inserted into the animal body, and the other terminal is connected to the attachment pad that attaches (or adheres to) the animal's skin.
  • a chemical solution such as a DNA vaccine or the like
  • electroporation syringe it is possible to inject a chemical solution such as a DNA vaccine or the like through an electroporation syringe and simultaneously apply an electric pulse from the skin of an animal to a terminal portion formed on the needle.
  • the electric pulse can be accurately transmitted to the desired site, so that the DNA vaccine material can be efficiently and safely delivered into the cell.
  • electrical stimulation can be applied at the same time with injection needles at a plurality of positions, thereby further increasing the effect of electroporation.
  • both the (+) and (-) terminals of the electric generator are configured with one needle, the equipment is further simplified, so product miniaturization is possible, production cost can be reduced, and convenience of use can be improved. .
  • stimulation applied to an animal can be minimized compared to a case in which a syringe for injecting a chemical solution such as a DNA vaccine material and a syringe for providing electrical stimulation are simultaneously used as in the prior art.
  • FIG. 1 is a conceptual diagram of a configuration according to an embodiment of an electroporation syringe according to the present invention
  • Figure 2 is a conceptual diagram of the configuration according to another embodiment of the electroporation syringe according to the present invention.
  • FIG. 3 is a conceptual diagram showing an electrical stimulation pattern according to the configuration of FIG. 2;
  • FIG. 4 is a conceptual diagram showing another embodiment of the present inventors electroporation syringe and an injection system for electroporation using the same,
  • FIG. 5 is a conceptual diagram illustrating a state in which a plurality of first non-coated portions and an attachment pad are respectively configured in the configuration of FIG. 4;
  • Figure 6 is a schematic diagram of a configuration according to another embodiment of the present inventors electroporation injector and an electroporation injection system using the same,
  • FIG. 7 is a conceptual diagram showing a state in which a plurality of (+) terminals are configured in the configuration of FIG. 6;
  • first, second, A, B, (a), and (b) may be used. These terms are only used to distinguish the component from other components, and the nature, order, or order of the component is not limited by the term.
  • FIG. 1 is a conceptual diagram of the configuration according to an embodiment of an electroporation syringe 20 according to the present invention
  • Figure 2 is a conceptual diagram of the configuration according to another embodiment of the electroporation syringe 20 according to the present invention
  • Figure 3 Is a conceptual diagram showing an electrical stimulation pattern according to the configuration of FIG. 2.
  • the electroporation syringe 20 includes a body 21, a coupler 22, an injection needle 23, and a connector 24.
  • the body 21 is a part into which the chemical liquid entering the body of the animal is introduced.
  • the chemical solution may be injected into the body through the rear or side surfaces of the body.
  • the electroporation syringe 20 may be in the form of a gun, for example. Since the shape and operation method of the gun-type syringe are already known techniques, detailed descriptions thereof will be omitted.
  • a coupler 22 is connected to the lower portion of the body 21.
  • the coupler 22 is formed on the body 21 and is a portion to which the injection needle 23 is detached.
  • the coupling type of the coupler 22 and the injection needle 23 has a known male and female coupling structure.
  • a helical hole (not shown) may be provided in the coupler 22, and a protrusion (not shown) may be provided at the upper circumference of the injection needle 23 to correspond to the helical hole. . That is, the injection needle 23 is rotated forward to fasten the protrusion to the helical hole, and the injection needle 23 is rotated in reverse to separate from the helical hole.
  • the coupler 22 and the injection needle 23 are sealedly coupled to each other so that the chemical solution does not leak to the fastened portion.
  • FIG. 1 shows a structure in which two injection needles 23 are coupled. That is, under the coupler 22, two coupling portions (not shown in the drawings) for coupling the injection needle 23 are formed. The coupling parts are spaced apart by a certain distance. An injection needle 23 is fastened to each coupling part.
  • the injection needle 23 is connected to the body 21 through a coupler 22.
  • the coupler 22 receives the drug solution from the body 21, and the delivered drug solution is injected into the body of the animal through the injection needle 23.
  • the injection needle 23 may have a form in which a lower end is opened and a chemical solution flows through the open lower end.
  • chemical solution discharge holes 23-5 may be formed on the circumferential surfaces of the two injection needles 23 facing each other.
  • the chemical liquid discharge hole 23-5 is composed of at least one hole.
  • the chemical liquid discharge hole 23-5 may be formed in at least one position. .
  • the chemical solution discharge holes 23-5 may be formed outside the two injection needles 23, respectively, not facing each other.
  • a connector 24 connected to at least one of the (+) terminal and the (-) terminal of the electric generator 10 is formed on one of the coupler 22 and the injection needle 23.
  • the connector 24 may be exposed to the outside or may be provided inside.
  • 1 conceptually illustrates a state in which the connector 24 is formed inside the coupler 22.
  • the connector 24 Through the connector 24, one of the two needles 23 is connected to the (+) terminal of the electric generator 10, and the other injection needle 23 is connected to the electric generator ( It is connected to the (-) terminal of 10).
  • it is a structure in which a chemical solution is delivered into cells located between the two injection needles 23 while applying electrical stimulation through the two injection needles 23.
  • FIG. 2 The configuration of FIG. 2 is the same as that of FIG. 1, but has a structure coated with an insulating material on the outer peripheral surface of the injection needle 23. That is, as shown in FIG. 2, a first coating portion 23-1 coated with a non-toxic insulating material is formed on the outer peripheral surface of the injection needle 23. In addition, a first non-coated part 23-2 that is not coated with a non-conductive insulating material is formed on the first coated part 23-1 inserted into the body of the animal. 2 shows that the first uncoated portion 23-2 is formed under the injection needle 23. The first uncoated portion 23-2 may be formed at a plurality of positions. The first uncoated part 23-2 is formed with a chemical liquid discharge hole 23-5 composed of at least one hole.
  • the chemical solution discharge hole 23-5 formed in the first uncoated part 23-2 may also be formed in at least one place of the first uncoated part 23-2, that is, at a plurality of locations, although not shown. .
  • the chemical liquid discharge hole 23-5 is composed of at least one hole and may be formed in a plurality of positions without a separate description.
  • FIG. 4 is a conceptual diagram showing another embodiment of an electroporation syringe 20 according to the present invention and an injection system for electroporation using the same
  • FIG. 5 is a first non-coated part 23-2 in the configuration of FIG. It is a conceptual diagram of a state in which a plurality of attachment pads 30 are respectively configured.
  • an electroporation syringe 20 is composed of a body 21, a coupler 22, an injection needle 23 and a connector 24, and an injection needle ( 23) consists of one.
  • an injection needle ( 23) consists of one.
  • FIGS. 1 and 2 For a basic configuration state, the description of FIGS. 1 and 2 may be referred to.
  • the injection needle 23 is composed of a conductor that conducts electricity.
  • a connector 24 is formed on the upper portion of the injection needle 23 connected to the body 21.
  • the connection structure between the connector 24 and the injection needle 23 can be applied to various known connection methods, and the injection needle 23 and the connector 24 are electrically connected.
  • One of the (+) terminal and the (-) terminal of the electric generator 10 may be connected to the connector 24.
  • the connector 24 may be formed on the coupler 22 instead of the injection needle 23.
  • the shape of the connector 24 is shown only conceptually, and the shape and configuration position thereof may be variously implemented at the level of those skilled in the art.
  • the entire circumferential surface of the injection needle 23 except for the first non-coated part 23-2 is coated with a non-toxic insulating material to form the first coated part 23-1.
  • the first uncoated portion 23-2 may be formed at one lower portion of the injection needle 23, or may be formed at the middle or upper portion of the injection needle 23 although not shown.
  • it may be formed at least in a plurality of positions (the middle part and the lower part), and the formation position is a position to deliver a drug solution (hereinafter referred to as'DNA vaccine') such as a DNA vaccine material. It can be configured in correspondence.
  • the length of the injection needle 23 can be standardized and manufactured differently, and the first uncoated portion 23-2 can also be standardized and mass-produced. Therefore, according to the animal species or the site where the DNA vaccine is to be injected, the standardized injection needle (23) is replaced and used, or the electroporation syringe (20) and the injection needle (23) are integrally standardized and mass produced.
  • the common syringe 20 itself may be replaced and used.
  • the injection needle 23 When the DNA vaccine is injected, the injection needle 23 is inserted into the body of the animal, and the DNA vaccine of the body 21 is injected into the body of the animal through the injection needle 23.
  • the first non-coated part 23-2 is configured to be located in the body of the animal when the injection needle 23 is inserted into the body of the animal.
  • the electroporation injection system using such an electroporation syringe 20 includes an electric generator 10, an electroporation syringe 20, and an attachment pad 30.
  • the electricity generator 10 generates a constant current or a constant voltage, and (+) and (-) terminals are formed. That is, 220 volt commercial power is applied and a constant current or constant voltage is output to generate an electric pulse (square-wave, square wave).
  • a constant current or constant voltage it is preferable to use a constant current without a change in the electric pulse, since the loss of the electric pulse may cause damage to the cell tissue as well as the delivery efficiency of the intracellular DNA vaccine may be reduced.
  • the electroporation syringe 20 is for injecting a DNA (DeoxyriboNucleic Acid) vaccine into the body of an animal.
  • a DNA DeoxyriboNucleic Acid
  • the injection needle 23 and the body 21 to which the DNA vaccine is injected are used with a medical hose, etc. It includes a connected type of ring gel, etc.
  • it will include all known medical items in the form of a connection with an injection needle 23 capable of injecting a DNA vaccine.
  • the attachment pad 30 is connected to the other terminal of the (+) terminal and the (-) terminal of the electric generator 10.
  • the (+) terminal of the electric generator 10 is connected to the attachment pad 30. Is connected through a cable 40.
  • the attachment pad 30 may be configured as one as shown in FIG. 4 or may be configured as a plurality as shown in FIG. 5.
  • the (+) terminal of the electric generator 10 is connected to each of the attachment pads 30 through respective cables 40.
  • the attachment pad 30 may also be attached to the opposite side of the skin 1 on which the 23 is inserted, so that electric stimulation may be applied from all directions.
  • the surface of the attachment pad 30 attached to the animal's skin 1 is made of a conductive material and has a structure that is connected to the (+) terminal of the electric generator 10.
  • a conductive adhesive for attaching to the skin 1 of an animal may be formed on the surface of the attachment pad 30.
  • a chemical solution discharge hole 23-5 communicating with the inside of the injection needle 23 may be formed in the first uncoated part 23-2.
  • the chemical liquid discharge holes 23-5 may be formed on both sides of the circumference of the first uncoated part 23-2, or may be formed in all directions. Since the DNA vaccine is discharged from a plurality of locations through the drug solution discharge hole 23-5 formed as described above, it is possible to induce the DNA vaccine to be well delivered into the cell with electric pulse stimulation.
  • FIG. 6 is a conceptual diagram showing a configuration according to another embodiment of an electroporation syringe 20 and an electroporation injection system using the same, and FIG. 7 shows a state in which a plurality of (+) terminals are configured in the configuration of FIG. It is a conceptual diagram.
  • the electroporation syringe 20 has a structure in which both terminals (+) and (-) of the electric generator 10 are connected to the injection needle 23.
  • the first coated part 23, the first uncoated part 23-2, the two terminal forming parts 24, the second coated part 23-3, and the second non-coated part on the injection needle 23 It includes part (23-4).
  • a connector 24 connected to any one of the positive and negative terminals of the electric generator 10 is electrically connected to the injection needle 23 of the electroporation syringe 20.
  • the first coating part 23-1 is coated with a non-toxic insulating material surrounding the outer peripheral surface of the injection needle 23 except for the connection part of the cable 40 of the connector 24.
  • at least one first non-coated portion 23-2 which is not coated with a non-toxic insulating material, is present at a site inserted into the body of the animal. That is, as in the configuration of FIG. 5, a plurality of first uncoated portions 23-2 may be formed.
  • the first uncoated portion 23-2 has a chemical solution discharge hole 23-5 communicating with the inside of the injection needle 23.
  • the terminal forming part 25 is formed on the outer circumferential surface of the first coating part 23-1.
  • the connector 24 is also connected to the terminal forming unit 25, and the terminal forming unit 25 through the connector 24 is connected to the other terminal among the (+) and (-) terminals of the electric generator 10. Connected. For example, if the (-) terminal is connected to the connector 24 connected to the injection needle 23, the (+) terminal is connected to the connector 24 connected to the terminal forming part 25.
  • the terminal forming part 25 is formed on a part of the circumferential surface of the first coated part 23-1, and does not interfere with the first uncoated part 23-2.
  • the terminal forming part 25 may be formed of a metal material surrounding the first coating part 23-1 and may be connected to the connector 24 or may be formed of a wire electrically connected to the connector 24.
  • the connector 24 formed on the injection needle 23 may be configured inside the coupler 22. Meanwhile, the connector 24 connected to the injection needle 23 and the connector 24 connected to the terminal forming part 25 are not electrically connected to each other.
  • the (+) terminal of the electric generator 10 may be connected to the connector 24 connected to the terminal forming part 25 as well as an attachment pad 30 additionally.
  • the attachment pad 30 may be connected to the opposite side of the skin 1 into which the injection needle 23 is inserted, as shown by the double-dashed line in FIG. 5. That is, it is possible to increase the treatment effect and reduce tissue damage by configuring to apply electrical stimulation from a plurality of locations to one place.
  • the second coating portion 23-3 is coated with a non-toxic insulating material on the outer peripheral surface of the terminal forming portion 25.
  • a second non-coated part 23-4 which is not coated with a non-toxic insulating material, is present. Meanwhile, in the drawing, a state in which the second uncoated portion 23-4 is configured as one under the terminal forming portion 25 is shown.
  • a chemical liquid discharge hole composed of at least one hole may be formed in the first uncoated part 23-2.
  • the non-toxic insulating material applied to the present invention is preferably made of an insulating material harmless to animals such as non-toxic silicone.
  • the electric generator 10 applied to the present invention can be used by connecting with a polarity controller (not shown) to change the (+) polarity and the (-) polarity. This is a known technique, so a detailed description thereof will be omitted. do.
  • DNA vaccine is injected into the body 21.
  • a cable 40 is connected between the connector 24 formed on the injection needle 23 and the (-) terminal of the electric generator 10.
  • the injection needle 23 of the electroporation syringe 20 is inserted into the body of the animal.
  • the attachment pad 30 forms a (+) pole
  • the first uncoated portion 23-2 of the injection needle 23 forms a (-) pole.
  • the animal's skin (1) located between the (+) and (-) poles, acts as a kind of resistance and forms a closed loop as a whole. Accordingly, as an electric pulse is applied from the attachment pad 30 to the lower uncoated portion 23-2 of the injection needle 23, a passage is temporarily formed in the peripheral cell membrane of the injection needle 23.
  • the DNA vaccine introduced into the body of the animal from the body 21 through the injection needle 23 penetrates into the cytoplasm and the cell nucleus through the passage temporarily formed as described above.
  • DNA vaccine is injected into the body 21.
  • a cable 40 is connected between the connector 24 formed on the terminal forming part 24 and the (+) terminal of the electric generator 10.
  • the medical syringe 20 is inserted into the animal body.
  • the terminal forming part 25 forms a (+) pole
  • the lower first uncoated part 23-2 of the injection needle 23 forms a (-) pole.
  • the animal skin (1) located between the (+) and (-) poles acts as a kind of resistance and forms a closed loop as a whole.
  • the injection needle (23) A passage is temporarily formed in the surrounding cell membrane.
  • the DNA vaccine introduced into the body of the animal from the body 21 through the injection needle 23 penetrates into the cytoplasm and the cell nucleus through the passage temporarily formed as described above.
  • the DNA vaccine injection and electric pulse stimulation can be performed through one injection needle 23, stimulation due to insertion of the injection needle 23 in the animal can be reduced.
  • the overall configuration of the electroporation syringe 20 and the injection system using the same is simplified, the volume can be reduced and convenience of use can be improved.
  • the formation position of the first non-coating part 23-2 to correspond to the position to which the DNA vaccine is to be delivered, the therapeutic effect can be improved and the DNA vaccine delivery efficiency can be further improved.

Abstract

The present invention relates to an electroporation syringe and an electroporation injection system using same, the electroporation syringe being configured to intensively deliver electrical stimulation to a local area in order to safely and efficiently deliver a drug solution such as a DNA vaccine material into cells. More specifically, the present invention relates to an electroporation syringe and an electroporation injection system using same, the electroporation syringe comprising: a body into which a drug solution entering an animal's body flows; a coupler formed on the body; an injection needle connected to the body through the coupler, and receiving the drug solution from the body and injecting same into the animal's body; and a connector formed on any one of the coupler or the injection needle and connected to at least one from among the (+) terminal and the (-) terminal of an electric generator.

Description

전기천공용 주사기 및 이를 이용한 전기천공용 주사 시스템Electroporation syringe and electroporation injection system using the same
본 발명은 전기천공용 주사기 및 이를 이용한 전기천공용 주사 시스템에 관한 것으로서, 특히, DNA 백신 물질 등과 같은 약액을 안전하고 효율적으로 세포 내로 전달하기 위해 전기 자극을 국소 부위에 집중적으로 전달할 수 있도록 구성한 전기천공용 주사기 및 이를 이용한 전기천공용 주사 시스템에 관한 것이다.The present invention relates to a syringe for electroporation and an injection system for electroporation using the same.In particular, in order to safely and efficiently deliver a drug such as a DNA vaccine material into cells, an electric stimulation configured to be intensively delivered to a local area It relates to a perforation syringe and an electroporation injection system using the same.
전기천공법(EP : Electroporation)이란 전류를 발생시켜 DNA(DeoxyriboNucleic Acid) 플라스미드를 세포 내로 전달하는 기술이다. 이 기술은 전기 펄스(square-wave, 구형파)를 사용하여 세포막에 일시적으로 통로를 만들어 DNA 플라스미드를 세포질과 세포핵 안까지 유입시킨다. 이는 DNA 백신 물질을 전달하는 방법 중 가장 효율적이며 안전하게 세포 내로 전달하는 기술로 각광받고 있다.Electroporation (EP) is a technology that generates electric current and delivers DNA (DeoxyriboNucleic Acid) plasmid into cells. This technique uses electric pulses (square-wave) to create a temporary passage through the cell membrane, which injects the DNA plasmid into the cytoplasm and the cell nucleus. This is the most efficient and safe way to deliver DNA vaccine materials into cells.
DNA 백신은 치료 물질에 대한 정보를 담고 있는 DNA와 이를 전달하기 위한 플라스미드(벡터)로 구성된 물질이다. 이와 같은 DNA 백신을 전기천공법을 통해 동물의 체내로 투여함으로써 유전자의 세포 내 전달 효율이 향상되고, 동물 종별에 따라 최적으로 설계됨으로써 CD4+, CD8+ T 세포 반응(세포성면역) 및 항체 형성 반응(체액성면역) 등 면역 능력 형성에 있어서 커다란 효과를 나타낸다.A DNA vaccine is a substance composed of DNA that contains information about a therapeutic substance and a plasmid (vector) to deliver it. By administering such a DNA vaccine into the body of an animal through electroporation, the efficiency of intracellular delivery of genes is improved, and by optimally designed according to the animal species, CD4 + , CD8 + T cell responses (cellular immunity) and antibody formation It has a great effect on the formation of immune capacity such as reaction (humoral immunity).
한편, 현재 전기천공법을 위해 사용되는 주사기의 경우 전술한 DNA 백신(약액)을 투여하기 위한 일반적인 주사기와 전기 펄스를 발생시키기 위한 별도의 주사 장치를 함께 사용해야만 한다.Meanwhile, in the case of a syringe currently used for electroporation, a general syringe for administering the aforementioned DNA vaccine (drug solution) and a separate injection device for generating an electric pulse must be used together.
이는 장비의 전체적인 부피를 늘리는 원인 중 하나가 되었으며, 사용상의 번거로움과 불편함을 초래한다. 또한, 종래의 장비로는 원하는 부위로 정확히 전기 펄스를 전달하는데 한계가 있다.This has become one of the causes of increasing the overall volume of the equipment, and causes inconvenience and inconvenience in use. In addition, there is a limitation in accurately delivering electric pulses to a desired site with conventional equipment.
본 발명이 해결하고자 하는 기술적 과제는, DNA 백신 등과 같은 약액 주입 및 전기 펄스 발생을 하나의 주사기를 통해 동시에 진행할 수 있도록 구성한 전기천공용 주사기 및 이를 이용한 전기천공용 주사 시스템을 제공하는데 있다.The technical problem to be solved by the present invention is to provide a syringe for electroporation and an injection system for electroporation using the same, and a syringe for electroporation configured to simultaneously proceed with injection of a chemical solution such as a DNA vaccine and generation of an electric pulse through one syringe.
본 발명이 해결하고자 하는 다른 기술적 과제는, 원하는 부위(특히, 피부 깊숙한 부위의 특정 위치)에 전기 펄스 자극을 가함과 동시에 특정 부위 이외의 피부 손상을 최소화하도록 구성한 전기천공용 주사기 및 이를 이용한 전기천공용 주사 시스템을 제공하는데 있다.Another technical problem to be solved by the present invention is an electroporation syringe configured to minimize skin damage other than a specific area while simultaneously applying electric pulse stimulation to a desired area (especially a specific location in a deep skin area), and an electric cloth using the same. It is to provide a common injection system.
상기 기술적 과제를 달성하기 위한 본 발명인 전기천공용 주사기는, 동물에게 사용하며 DNA 백신 물질을 세포 내로 전달하기 위한 전기천공용 주사기에 관한 것으로, 동물의 체내로 들어가는 약액이 유입되는 몸체; 상기 몸체에 형성되는 커플러; 상기 커플러를 통해 상기 몸체와 연결되며, 상기 몸체로부터 약액을 전달받아 동물의 체내로 주입되는 주사바늘; 및 상기 커플러와 상기 주사바늘 중 어느 하나에 형성되며, 전기발생기의 (+)단자와 (-)단자 중 적어도 어느 하나의 단자와 연결되는 커넥터;를 포함하는 것을 특징으로 한다.An electroporation syringe according to the present invention for achieving the above technical problem relates to an electroporation syringe for use in an animal and for delivering a DNA vaccine material into cells, comprising: a body into which a chemical liquid entering the body of the animal is introduced; A coupler formed on the body; An injection needle connected to the body through the coupler and receiving a chemical solution from the body and injected into the body of the animal; And a connector formed on one of the coupler and the injection needle and connected to at least one of the (+) terminal and the (-) terminal of the electric generator.
또한, 동물의 체내로 삽입되는 상기 주사바늘의 측면 둘레부에는, 적어도 하나의 홀로 구성된 약액배출공;이 더 구비되는 것을 특징으로 한다.In addition, a chemical solution discharge hole composed of at least one hole; is further provided at a side circumference of the injection needle inserted into the body of the animal.
또한, 상기 주사바늘의 외측 둘레면에는 무독성 절연물질로 코팅된 제1코팅부가 형성되고, 동물의 체내로 삽입되는 상기 제1코팅부에는 상기 무독성 절연물질이 코팅되지 않은 제1비코팅부가 적어도 한 곳에 형성되며, 상기 제1비코팅부에는 적어도 하나의 홀로 구성된 약액배출공이 더 구비되는 것을 특징으로 한다.In addition, a first coating part coated with a non-toxic insulating material is formed on the outer circumferential surface of the injection needle, and at least one first non-coated part not coated with the non-toxic insulating material is formed on the first coating part inserted into the body of the animal. It is formed in the position, characterized in that the first non-coated portion is characterized in that further provided with a chemical liquid discharge hole consisting of at least one hole.
또한, 상기 제1코팅부의 외측 둘레면에 결합되며, 도전성 재질로 구성되어 상기 커넥터가 결합되는 단자형성부; 및 상기 단자형성부의 외측 둘레면에 무독성 절연물질로 코팅된 제2코팅부;를 더 포함하고, 동물의 체내로 삽입되는 상기 제2코팅부에 상기 무독성 절연물질로 코팅되지 않은 제2비코팅부가 적어도 한 곳에 형성되는 것을 특징으로 한다.In addition, a terminal forming portion coupled to the outer circumferential surface of the first coating portion and formed of a conductive material to which the connector is coupled; And a second coating part coated with a non-toxic insulating material on the outer circumferential surface of the terminal forming part, and a second non-coating part not coated with the non-toxic insulating material on the second coating part inserted into the body of the animal It is characterized in that it is formed in at least one place.
또한, 상기 커플러는, 두 개로 형성되어 커플러 상호간이 절연처리되며, 어느 하나의 커플러는 전기발생기의 (+)단자와 연결되고, 나머지 다른 하나의 커플러는 전기발생기의 (-)단자와 연결되며, 각각의 상기 커플러에 상기 주사바늘이 결합되는 것을 특징으로 한다.In addition, the coupler is formed of two, so that the couplers are insulated from each other, one coupler is connected to the (+) terminal of the electric generator, and the other coupler is connected to the (-) terminal of the electric generator, It characterized in that the injection needle is coupled to each of the couplers.
또한, 동물의 체내로 삽입되는 두 개의 상기 주사바늘 상호간에 마주보는 부위에는 적어도 하나의 홀로 구성된 약액배출공;이 더 구비되는 것을 특징으로 한다.In addition, the two injection needles inserted into the body of the animal face each other, a chemical solution discharge hole consisting of at least one hole; characterized in that further provided.
또한, 각각의 상기 주사바늘의 외측 둘레면에는 무독성 절연물질로 코팅된 제1코팅부가 형성되고, 동물의 체내로 삽입되는 상기 제1코팅부에는 상기 무독성 절연물질이 코팅되지 않은 제1비코팅부가 적어도 한 곳에 형성되며, 상기 제1비코팅부 상호간에 마주보는 부위에는 적어도 하나의 홀로 구성된 약액배출공이 더 구비되는 것을 특징으로 한다.In addition, a first coating part coated with a non-toxic insulating material is formed on the outer circumferential surface of each of the injection needles, and the first non-coated part not coated with the non-toxic insulating material is formed on the first coating part inserted into the body of the animal. It is formed in at least one place, characterized in that a chemical liquid discharge hole formed of at least one hole is further provided in a portion facing each other in the first uncoated portion.
한편, 본 발명인 전기천공용 주사기를 이용한 전기천공용 주사 시스템의 일 실시례에 의하면, 전기발생기; 동물의 체내로 들어가는 약액이 유입되는 몸체와, 상기 몸체에 형성되는 커플러와, 상기 커플러를 통해 상기 몸체와 연결되며 상기 몸체로부터 약액을 전달받아 동물의 체내로 주입하는 주사바늘과, 상기 커플러와 상기 주사바늘 중 어느 하나에 형성되며 상기 전기발생기의 (+)단자와 (-)단자 중 어느 하나의 단자에 연결되는 커넥터를 포함하는 전기천공용 주사기; 및 상기 전기발생기의 (+)단자와 (-)단자 중 나머지 다른 하나의 단자와 연결되어 동물의 피부에 부착되는 적어도 하나의 부착패드;를 포함하며, 상기 전기천공용 주사기는, 상기 주사바늘의 외측 둘레면에는 무독성 절연물질로 코팅된 제1코팅부가 형성되고, 동물의 체내로 삽입되는 상기 제1코팅부에는 상기 무독성 절연물질이 코팅되지 않은 제1비코팅부가 적어도 한 곳에 형성되는 것을 특징으로 한다.On the other hand, according to an embodiment of the injection system for electroporation using an electroporation syringe of the present invention, the electric generator; A body through which a chemical liquid entering the body of an animal flows into the body, a coupler formed in the body, an injection needle connected to the body through the coupler and receiving the chemical liquid from the body and injecting it into the body of the animal, the coupler and the An electroporation syringe formed on any one of the injection needles and including a connector connected to any one of the (+) terminal and the (-) terminal of the electric generator; And at least one attachment pad connected to the other one of the (+) terminal and the (-) terminal of the electric generator and attached to the skin of the animal, wherein the electroporation syringe comprises: of the injection needle. A first coating portion coated with a non-toxic insulating material is formed on the outer circumferential surface, and a first non-coated portion not coated with the non-toxic insulating material is formed on at least one portion of the first coating portion inserted into the body of the animal. do.
또한, 상기 전기천공용 주사기는, 동물의 체내로 삽입되는 상기 주사바늘의 측면 둘레부에 적어도 하나의 홀로 구성된 약액배출공이 더 구비되는 것을 특징으로 한다.In addition, the electroporation syringe is characterized in that it is further provided with a chemical solution discharge hole composed of at least one hole at the circumference of the side of the injection needle inserted into the body of the animal.
또한, 상기 전기발생기의 (+)극성과 (-)극성 상호간을 변경하여 전달하는 극성제어기를 더 포함하는 것을 특징으로 한다. In addition, it is characterized in that it further comprises a polarity controller for transmitting by changing the (+) polarity and (-) polarity of the electric generator.
또한, 본 발명인 전기천공용 주사기를 이용한 전기천공용 주사 시스템의 다른 실시례에 의하면, 전기발생기; 및 동물의 체내로 들어가는 약액이 유입되는 몸체와, 상기 몸체에 형성되는 커플러와, 상기 커플러를 통해 상기 몸체와 연결되며 상기 몸체로부터 약액을 전달받아 동물의 체내로 주입하는 주사바늘과, 상기 커플러와 상기 주사바늘 중 어느 하나에 형성되며 상기 전기발생기의 (+)단자와 (-)단자 중 적어도 어느 하나의 단자에 연결되는 커넥터를 포함하는 전기천공용 주사기;를 포함하며, 상기 전기천공용 주사기는, 상기 전기발생기의 (+)단자와 (-)단자 중 어느 하나의 단자와 연결되는 상기 주사바늘의 외측 둘레면에는 무독성 절연물질로 코팅된 제1코팅부가 형성되고, 동물의 체내로 삽입되는 상기 제1코팅부에는 상기 무독성 절연물질이 코팅되지 않은 제1비코팅부가 적어도 한 곳에 형성되며, 상기 제1코팅부의 외측 둘레면에 결합되며, 도전성 재질로 구성되어 상기 전기발생기의 (+)단자와 (-)단자 중 나머지 다른 하나의 단자와 연결되도록 상기 커넥터가 결합되는 단자형성부; 및 상기 단자형성부의 외측 둘레면에 무독성 절연물질로 코팅된 제2코팅부;를 더 포함하고, 동물의 체내로 삽입되는 상기 제2코팅부에는 상기 무독성 절연물질로 코팅되지 않은 제2비코팅부가 적어도 한 곳에 형성되는 것을 특징으로 한다.In addition, according to another embodiment of the electroporation injection system using the electroporation syringe of the present invention, the electric generator; And a body through which a chemical liquid entering the body of an animal is introduced, a coupler formed in the body, an injection needle connected to the body through the coupler and receiving the chemical liquid from the body and injecting it into the body of the animal, and the coupler. Includes; an electroporation syringe formed in any one of the injection needles and including a connector connected to at least one of the (+) terminal and the (-) terminal of the electric generator, wherein the electroporation syringe , A first coating part coated with a non-toxic insulating material is formed on the outer circumferential surface of the injection needle connected to any one of the (+) terminal and the (-) terminal of the electric generator, and is inserted into the body of the animal. The first non-coated part, which is not coated with the non-toxic insulating material, is formed on at least one part of the first coating part, is coupled to the outer circumferential surface of the first coating part, and is made of a conductive material, so that the (+) terminal of the electric generator and the A terminal forming part to which the connector is coupled so as to be connected to the other one of the (-) terminals; And a second coating part coated with a non-toxic insulating material on the outer circumferential surface of the terminal forming part, wherein the second coating part inserted into the body of the animal has a second non-coated part not coated with the non-toxic insulating material It is characterized in that it is formed in at least one place.
또한, 상기 전기발생기의 (+)단자와 (-)단자 중 어느 하나의 단자와 연결되어 동물의 피부에 부착되는 적어도 하나의 부착패드;를 더 포함하는 것을 특징으로 한다.In addition, it characterized in that it further comprises at least one attachment pad connected to any one of the (+) terminal and the (-) terminal of the electric generator and attached to the skin of the animal.
또한, 상기 전기발생기의 (+)극성과 (-)극성 상호간을 변경하여 전달하는 극성제어기를 더 포함하는 것을 특징으로 한다.In addition, it is characterized in that it further comprises a polarity controller for transmitting by changing the (+) polarity and (-) polarity of the electric generator.
이상에서 상술한 본 발명에 의한 전기천공용 주사기 및 이를 이용한 전기천공용 주사 시스템은 다음과 같은 효과가 있다.The electroporation syringe and the electroporation injection system using the same according to the present invention described above have the following effects.
먼저, 주사바늘을 통해 전기 펄스를 인가함과 동시에 DNA 백신 등과 같은 약액을 주입하므로 종래 대비 사용 편의성 및 효율성을을 크게 향상시킬 수 있다.First, since an electric pulse is applied through an injection needle and a chemical solution such as a DNA vaccine is injected at the same time, the convenience and efficiency of use can be greatly improved compared to the prior art.
또한, 주사바늘의 측면 둘레부에 약액배출공을 형성하여 DNA 백신의 투여량을 늘리고 투여범위를 확대시킬 수 있으므로 세포내 DNA 백신 전달 효율을 향싱시킬 수 있다.In addition, since the drug solution discharge hole is formed in the lateral circumference of the injection needle, the dose of the DNA vaccine can be increased and the range of administration can be expanded, thereby enhancing the efficiency of intracellular DNA vaccine delivery.
또한, 주사바늘이 결합되는 커플러를 구성하여 다양한 형태의 주사바늘을 결합하여 사용할 수 있다.In addition, by configuring a coupler to which the injection needle is coupled, various types of injection needles can be combined and used.
또한, 커플러에 전기발생기와 연결되는 커넥터를 형성할 경우 주사바늘에 커넥터를 형성하는 것에 비해 제작이 용이함은 물론, 사용 편의성도 더욱 향상시킬 수 있다.In addition, when a connector connected to the electric generator is formed on the coupler, it is easier to manufacture as well as the convenience of use compared to forming the connector on the injection needle.
또한, 주사바늘을 두 개로 구성하고 어느 하나의 주사바늘에 전기발생기의 (+)단자를 연결하고, 다른 하나의 주사바늘에 (-)단자를 연결하여 사용함으로써, 주사바늘 사이에 위치한 세포내 DNA 백신 전달효율을 증대시킬 수 있다.In addition, by using two injection needles, connecting the (+) terminal of the electric generator to one of the injection needles, and connecting the (-) terminal to the other injection needle, intracellular DNA located between the injection needles It can increase vaccine delivery efficiency.
또한, 두 개의 주사바늘 상호간에 마주보는 위치에 약액배출공을 형성하여 사용할 경우 전술한 세포내 DNA 백신 전달효율을 더욱 증대시킬 수 있다. In addition, when two injection needles are used by forming a drug solution discharge hole at a position facing each other, the above-described intracellular DNA vaccine delivery efficiency can be further increased.
또한, 전기발생기의 (+), (-)양 단자 중 어느 하나의 단자를 동물 체내로 삽입되는 바늘에 형성하고, 나머지 다른 하나의 단자를 동물의 피부에 부착(또는 밀착)하는 부착패드에 연결하여 구성함으로써, 전기천공용 주사기를 통해 DNA 백신 등과 같은 약액을 주입함과 동시에 전기 펄스를 동물의 피부로부터 바늘에 형성된 단자 부위로 가할 수 있다.In addition, one of the (+) and (-) terminals of the electric generator is formed on the needle inserted into the animal body, and the other terminal is connected to the attachment pad that attaches (or adheres to) the animal's skin. Thus, it is possible to inject a chemical solution such as a DNA vaccine or the like through an electroporation syringe and simultaneously apply an electric pulse from the skin of an animal to a terminal portion formed on the needle.
또한, 주사바늘의 비코팅부를 전기 펄스를 가하기 위한 부위에 형성함으로써 원하는 부위에 정확히 전기 펄스를 보낼 수 있으므로, DNA 백신 물질을 세포 내로 효율적이고 안전하게 전달할 수 있다.In addition, by forming the uncoated portion of the injection needle at the site for applying the electric pulse, the electric pulse can be accurately transmitted to the desired site, so that the DNA vaccine material can be efficiently and safely delivered into the cell.
또한, 부착패드를 다수로 구성할 경우 다수의 위치에서 주사바늘로 동시에 전기적 자극을 가할 수 있으므로 전기천공법(Electroporation) 효과를 더욱 증대시킬 수 있다.In addition, when a plurality of attachment pads are configured, electrical stimulation can be applied at the same time with injection needles at a plurality of positions, thereby further increasing the effect of electroporation.
또한, 전기발생기의 (+), (-)양 단자 모두를 바늘 하나에 구성할 경우 장비가 더욱 간소화되므로 제품 소형화가 가능하며, 생산 단가를 절감시킬 수 있음은 물론, 사용 편의성을 향상시킬 수 있다.In addition, if both the (+) and (-) terminals of the electric generator are configured with one needle, the equipment is further simplified, so product miniaturization is possible, production cost can be reduced, and convenience of use can be improved. .
또한, 하나의 주사바늘을 사용할 경우, 종래와 같이 DNA 백신 물질 등과 같은 약액 주입을 위한 주사기와 전기적 자극을 주기 위한 주사기를 동시에 사용하는 경우에 비해 동물에게 가해지는 자극을 최소화시킬 수 있다.In addition, when one injection needle is used, stimulation applied to an animal can be minimized compared to a case in which a syringe for injecting a chemical solution such as a DNA vaccine material and a syringe for providing electrical stimulation are simultaneously used as in the prior art.
도 1은 본 발명에 따른 전기천공용 주사기의 일 실시례에 의한 구성 개념도,1 is a conceptual diagram of a configuration according to an embodiment of an electroporation syringe according to the present invention,
도 2는 본 발명에 따른 전기천공용 주사기의 다른 실시례에 의한 구성 개념도,Figure 2 is a conceptual diagram of the configuration according to another embodiment of the electroporation syringe according to the present invention,
도 3은 도 2의 구성에 따른 전기자극 패턴을 나타낸 개념도,3 is a conceptual diagram showing an electrical stimulation pattern according to the configuration of FIG. 2;
도 4는 본 발명인 전기천공용 주사기의 또 다른 실시례와 이를 이용한 전기천공용 주사 시스템을 나타낸 구성 개념도,4 is a conceptual diagram showing another embodiment of the present inventors electroporation syringe and an injection system for electroporation using the same,
도 5는 도 4의 구성에서 제1비코팅부와 부착패드를 각각 복수로 구성한 상태 개념도,FIG. 5 is a conceptual diagram illustrating a state in which a plurality of first non-coated portions and an attachment pad are respectively configured in the configuration of FIG. 4;
도 6은 본 발명인 전기천공용 주사기 및 이를 이용한 전기천공용 주사 시스템의 또 다른 실시례에 따른 구성 개념도,Figure 6 is a schematic diagram of a configuration according to another embodiment of the present inventors electroporation injector and an electroporation injection system using the same,
도 7은 도 6의 구성에서 (+)단자를 복수로 구성한 상태를 나타낸 개념도.7 is a conceptual diagram showing a state in which a plurality of (+) terminals are configured in the configuration of FIG. 6;
이하, 본 발명의 일부 실시례들을 예시적인 도면을 통해 상세하게 설명한다. 각 도면의 구성요소들에 참조부호를 부가함에 있어서, 동일한 구성요소들에 대해서는 비록 다른 도면상에 표시되더라도 가능한 한 동일한 부호를 가지도록 하고 있음에 유의해야 한다. 또한, 본 발명의 실시례를 설명함에 있어, 관련된 공지구성 또는 기능에 대한 구체적인 설명이 본 발명의 실시례에 대한 이해를 방해한다고 판단되는 경우에는 그 상세한 설명은 생략한다.Hereinafter, some embodiments of the present invention will be described in detail through exemplary drawings. In adding reference numerals to elements of each drawing, it should be noted that the same elements are assigned the same numerals as possible even if they are indicated on different drawings. In addition, in describing the embodiments of the present invention, if it is determined that a detailed description of a related known configuration or function interferes with the understanding of the embodiments of the present invention, the detailed description thereof will be omitted.
또한, 본 발명의 실시례의 구성 요소를 설명하는 데 있어서, 제 1, 제 2, A, B, (a), (b) 등의 용어를 사용할 수 있다. 이러한 용어는 그 구성 요소를 다른 구성 요소와 구별하기 위한 것일 뿐, 그 용어에 의해 해당 구성 요소의 본질이나 차례 또는 순서 등이 한정되지 않는다. 어떤 구성 요소가 다른 구성요소에 "연결", "결합" 또는 "접속"된다고 기재된 경우, 그 구성 요소는 그 다른 구성요소에 직접적으로 연결되거나 접속될 수 있지만, 각 구성 요소 사이에 또 다른 구성 요소가 "연결", "결합" 또는 "접속"될 수도 있다고 이해되어야 할 것이다.In addition, in describing the constituent elements of the embodiment of the present invention, terms such as first, second, A, B, (a), and (b) may be used. These terms are only used to distinguish the component from other components, and the nature, order, or order of the component is not limited by the term. When a component is described as being "connected", "coupled" or "connected" to another component, that component may be directly connected or connected to that other component, but another component between each component It should be understood that may be “connected”, “coupled” or “connected”.
먼저, 본 발명을 설명하기에 앞서 본 발명에 도시된 도면들은 본 발명을 설명하기 위한 개념도로써, 비율, 형상 및 크기 등은 실제와 다를 수 있으며 그 구조적 연결 관계만을 참조하도록 한다.First, prior to describing the present invention, the drawings shown in the present invention are conceptual diagrams for explaining the present invention, and the ratio, shape, and size may be different from the actual ones, and only the structural connection relationship is referred to.
도 1은 본 발명에 따른 전기천공용 주사기(20)의 일 실시례에 의한 구성 개념도이고, 도 2는 본 발명에 따른 전기천공용 주사기(20)의 다른 실시례에 의한 구성 개념도이며, 도 3은 도 2의 구성에 따른 전기자극 패턴을 나타낸 개념도이다.1 is a conceptual diagram of the configuration according to an embodiment of an electroporation syringe 20 according to the present invention, Figure 2 is a conceptual diagram of the configuration according to another embodiment of the electroporation syringe 20 according to the present invention, Figure 3 Is a conceptual diagram showing an electrical stimulation pattern according to the configuration of FIG. 2.
먼저, 도 1을 참조하면, 본 발명인 전기천공용 주사기(20)는 몸체(21), 커플러(22), 주사바늘(23) 및 커넥터(24)를 포함하여 구성된다.First, referring to FIG. 1, the electroporation syringe 20 according to the present invention includes a body 21, a coupler 22, an injection needle 23, and a connector 24.
몸체(21)는 동물의 체내로 들어가는 약액이 유입되는 부분이다. 약액은 몸체의 후면 또는 측면을 통해 몸체 내부로 주입될 수 있다. 전기천공용 주사기(20)의 전체적인 형태를 도시하진 않았으나, 전기천공용 주사기(20)는 일례로 건(Gun)의 형태일 수 있다. 건 형태의 주사기의 형상 및 동작 방식 등은 이미 공지의 기술이므로 이에 대한 자세한 설명은 생략하도록 한다.The body 21 is a part into which the chemical liquid entering the body of the animal is introduced. The chemical solution may be injected into the body through the rear or side surfaces of the body. Although the overall shape of the electroporation syringe 20 is not shown, the electroporation syringe 20 may be in the form of a gun, for example. Since the shape and operation method of the gun-type syringe are already known techniques, detailed descriptions thereof will be omitted.
도 1을 기준으로, 몸체(21)의 하부에는 커플러(22)가 연결된다. 커플러(22)는 몸체(21)에 형성되어 주사바늘(23)이 탈착되는 부분이다. 커플러(22)와 주사바늘(23)의 결합 형태는 공지의 암수 결합구조의 형태를 갖는다. 일례로, 커플러(22)에는 나선 형상의 홀(미도시)이 구비되고, 주사바늘(23)의 상부 둘레부에는 상기 나선 형상의 홀에 대응되어 체결되는 돌기(미도시)가 구비될 수 있다. 즉, 주사바늘(23)을 정회전시켜 상기 돌기를 상기 나선 형상의 홀에 체결하고, 주사바늘(23)을 역회전시켜 상기 나선 형상의 홀로부터 분리하는 구조이다. 체결시에는 커플러(22)와 주사바늘(23) 상호간이 밀폐결합되어 체결된 부위로 약액이 유출되지 않도록 구성하는 것이 바람직하다.Referring to FIG. 1, a coupler 22 is connected to the lower portion of the body 21. The coupler 22 is formed on the body 21 and is a portion to which the injection needle 23 is detached. The coupling type of the coupler 22 and the injection needle 23 has a known male and female coupling structure. For example, a helical hole (not shown) may be provided in the coupler 22, and a protrusion (not shown) may be provided at the upper circumference of the injection needle 23 to correspond to the helical hole. . That is, the injection needle 23 is rotated forward to fasten the protrusion to the helical hole, and the injection needle 23 is rotated in reverse to separate from the helical hole. When fastening, it is preferable that the coupler 22 and the injection needle 23 are sealedly coupled to each other so that the chemical solution does not leak to the fastened portion.
커플러(22)에는 하나 또는 두 개의 주사바늘(23)이 결합될 수 있으며, 도 1에는 두 개의 주사바늘(23)이 결합되는 구조를 도시하였다. 즉, 커플러(22)의 하부에는 주사바늘(23)이 결합되기 위한 결합부(도면부호 미기재)가 두 개 형성된다. 결합부 상호간은 일정간격 이격된 형태이다. 각각의 결합부에는 주사바늘(23)이 체결된다.One or two injection needles 23 may be coupled to the coupler 22, and FIG. 1 shows a structure in which two injection needles 23 are coupled. That is, under the coupler 22, two coupling portions (not shown in the drawings) for coupling the injection needle 23 are formed. The coupling parts are spaced apart by a certain distance. An injection needle 23 is fastened to each coupling part.
주사바늘(23)은 커플러(22)를 통해 몸체(21)와 연결된다. 커플러(22)는 몸체(21)로부터 약액을 전달받으며, 전달된 약액은 주사바늘(23)을 통해 동물의 체내로 주입된다. 주사바늘(23)은 일반적인 주사바늘과 같이 하단부가 개방되고, 개방된 하단부를 통해 약액이 유출되는 형태일 수 있다. 또는, 도 1에 도시된 바와 같이, 상호 마주보는 두 개의 주사바늘(23)의 둘레면에는 각각 약액배출공(23-5)이 형성될 수 있다. 약액배출공(23-5)은 적어도 하나의 홀로 구성된다. 또한, 도면상에는 약액배출공(23-5)을 두 개의 주사바늘(23)이 상호 마주보는 곳에만 형성된 것으로 도시하였으나, 약액배출공(23-5)은 적어도 어느 한 곳의 위치에 형성될 수 있다. 일례로, 미도시 하였으나, 약액배출공(23-5)은 두 개의 주사바늘(23)이 상호 마주하지 않는 바깥쪽에도 각각 형성될 수 있다.The injection needle 23 is connected to the body 21 through a coupler 22. The coupler 22 receives the drug solution from the body 21, and the delivered drug solution is injected into the body of the animal through the injection needle 23. Like a general injection needle, the injection needle 23 may have a form in which a lower end is opened and a chemical solution flows through the open lower end. Alternatively, as shown in FIG. 1, chemical solution discharge holes 23-5 may be formed on the circumferential surfaces of the two injection needles 23 facing each other. The chemical liquid discharge hole 23-5 is composed of at least one hole. In addition, although the figure shows that the chemical liquid discharge hole 23-5 is formed only where the two injection needles 23 face each other, the chemical liquid discharge hole 23-5 may be formed in at least one position. . For example, although not shown, the chemical solution discharge holes 23-5 may be formed outside the two injection needles 23, respectively, not facing each other.
또한, 커플러(22)와 주사바늘(23) 중 어느 하나에는 전기발생기(10)의 (+)단자와 (-)단자 중 적어도 어느 하나의 단자와 연결되는 커넥터(24)가 형성된다. 커넥터(24)는 외부로 노출되거나 내부에 구비된 형태일 수 있다. 도 1에는 커넥터(24)가 커플러(22)의 내부에 형성된 상태를 개념적으로 도시하였다. 커넥터(24)를 통해 두 개의 주사바늘(23) 중 어느 하나의 주사바늘(23)은 전기발생기(10)의 (+)단자와 연결되고, 나머지 다른 하나의 주사바늘(23)은 전기발생기(10)의 (-)단자와 연결된다. 즉, 두 개의 주사바늘(23)을 통해 전기자극을 가하면서 두 개의 주사바늘(23) 사이에 위치한 세포 내부로 약액을 전달시키는 구조이다.In addition, a connector 24 connected to at least one of the (+) terminal and the (-) terminal of the electric generator 10 is formed on one of the coupler 22 and the injection needle 23. The connector 24 may be exposed to the outside or may be provided inside. 1 conceptually illustrates a state in which the connector 24 is formed inside the coupler 22. Through the connector 24, one of the two needles 23 is connected to the (+) terminal of the electric generator 10, and the other injection needle 23 is connected to the electric generator ( It is connected to the (-) terminal of 10). In other words, it is a structure in which a chemical solution is delivered into cells located between the two injection needles 23 while applying electrical stimulation through the two injection needles 23.
도 2의 구성은 도 1의 구성과 같으며, 다만 주사바늘(23)의 외측 둘레면에 절연물질로 코팅된 구조를 갖는다. 즉, 도 2에 도시된 바와 같이, 주사바늘(23)의 외측 둘레면에는 무독성 절연물질로 코팅된 제1코팅부(23-1)가 형성된다. 또한, 동물의 체내로 삽입되는 제1코팅부(23-1)에는 무도성 절연물질로 코팅되지 않은 제1비코팅부(23-2)가 형성된다. 도 2에는 주사바늘(23)의 하부에 제1비코팅부(23-2)가 형성된 것으로 도시하였다. 제1비코팅부(23-2)는 복수 개의 위치에 형성할 수 있다. 제1비코팅부(23-2)에는 적어도 하나의 홀로 구성된 약액배출공(23-5)이 형성된다. 제1비코팅부(23-2)에 형성된 약액배출공(23-5)도, 미도시 하였으나, 제1비코팅부(23-2)의 적어도 한 곳, 즉 복수의 위치에 형성될 수 있다. 이하의 설명에서는 약액배출공(23-5)은 별도의 설명이 없어도 적어도 하나의 홀로 구성되고, 복수의 위치에 형성될 수 있는 것으로 이해할 수 있다.The configuration of FIG. 2 is the same as that of FIG. 1, but has a structure coated with an insulating material on the outer peripheral surface of the injection needle 23. That is, as shown in FIG. 2, a first coating portion 23-1 coated with a non-toxic insulating material is formed on the outer peripheral surface of the injection needle 23. In addition, a first non-coated part 23-2 that is not coated with a non-conductive insulating material is formed on the first coated part 23-1 inserted into the body of the animal. 2 shows that the first uncoated portion 23-2 is formed under the injection needle 23. The first uncoated portion 23-2 may be formed at a plurality of positions. The first uncoated part 23-2 is formed with a chemical liquid discharge hole 23-5 composed of at least one hole. The chemical solution discharge hole 23-5 formed in the first uncoated part 23-2 may also be formed in at least one place of the first uncoated part 23-2, that is, at a plurality of locations, although not shown. . In the following description, it can be understood that the chemical liquid discharge hole 23-5 is composed of at least one hole and may be formed in a plurality of positions without a separate description.
도 2와 같이 구성된 전기천공용 주사기(20)를 사용하면, 도 3에 도시된 바와 같이 두 개의 주사바늘(23) 사이로 전기자극을 가함과 동시에 약액배출공(23-5)을 통해 약액을 배출시키게 되므로, 배출된 약액을 세포내로 효율적으로 전달할 수 있다.When the electroporation syringe 20 configured as shown in FIG. 2 is used, an electrical stimulation is applied between the two injection needles 23 as shown in FIG. 3 and the chemical solution is discharged through the chemical solution discharge hole 23-5. Therefore, the discharged chemical solution can be efficiently delivered into the cells.
도 4는 본 발명인 전기천공용 주사기(20)의 또 다른 실시례와 이를 이용한 전기천공용 주사 시스템을 나타낸 구성 개념도이며, 도 5는 도 4의 구성에서 제1비코팅부(23-2)와 부착패드(30)를 각각 복수로 구성한 상태 개념도이다.FIG. 4 is a conceptual diagram showing another embodiment of an electroporation syringe 20 according to the present invention and an injection system for electroporation using the same, and FIG. 5 is a first non-coated part 23-2 in the configuration of FIG. It is a conceptual diagram of a state in which a plurality of attachment pads 30 are respectively configured.
도 4를 참조하면, 본 발명의 또 다른 실시례에 의한 전기천공용 주사기(20)는 몸체(21), 커플러(22), 주사바늘(23) 및 커넥터(24)로 구성되며, 주사바늘(23)이 한 개로 구성된다. 기본적인 구성 상태는 도 1 및 도 2의 설명을 참조할 수 있다. 4, an electroporation syringe 20 according to another embodiment of the present invention is composed of a body 21, a coupler 22, an injection needle 23 and a connector 24, and an injection needle ( 23) consists of one. For a basic configuration state, the description of FIGS. 1 and 2 may be referred to.
주사바늘(23)은 전기가 통하는 도체로 구성된다. 몸체(21)와 연결된 주사바늘(23)의 상부에는 커넥터(24)가 형성된다. 커넥터(24)와 주사바늘(23)의 연결 구조는 공지된 다양한 연결 방식을 적용할 수 있으며, 주사바늘(23)과 커넥터(24)는 전기적으로 연결된다. 커넥터(24)에는 전기발생기(10)의 (+)단자와 (-)단자 중 어느 하나의 단자가 연결될 수 있다. 커넥터(24)는 주사바늘(23)이 아닌 커플러(22)에 형성할 수도 있다. 도면상에는 커넥터(24)의 형상을 개념적으로만 도시한 것으로써, 그 형상과 구성 위치는 당업자의 수준에서 다양하게 구현할 수 있을 것이다. The injection needle 23 is composed of a conductor that conducts electricity. A connector 24 is formed on the upper portion of the injection needle 23 connected to the body 21. The connection structure between the connector 24 and the injection needle 23 can be applied to various known connection methods, and the injection needle 23 and the connector 24 are electrically connected. One of the (+) terminal and the (-) terminal of the electric generator 10 may be connected to the connector 24. The connector 24 may be formed on the coupler 22 instead of the injection needle 23. In the drawings, the shape of the connector 24 is shown only conceptually, and the shape and configuration position thereof may be variously implemented at the level of those skilled in the art.
제1비코팅부(23-2)를 제외한 주사바늘(23)의 둘레면 전체는 무독성 절연물질로 코팅되어 제1코팅부(23-1)를 형성한다. 제1비코팅부(23-2)는 도 4에 도시된 바와 같이 주사바늘(23)의 하부 한 곳에 형성하거나, 미도시 하였으나 주사바늘(23)의 중단부나 상부에 형성할 수도 있다. 또한, 도 5에 도시된 바와 같이, 적어도 복수의 위치(중단부와 하단부)에 형성할 수도 있으며, 그 형성 위치는 DNA 백신 물질 등과 같은 약액(이하, 'DNA 백신'이라 함)을 전달할 위치에 대응시켜 구성할 수 있다. 즉, 제1비코팅부(23-2)로 전기펄스가 가해지므로 제1비코팅부(23-2)를 DNA 백신을 전달할 위치에 형성할 경우 전기천공법(EP : Electroporation) 효율을 향상시킬 수 있다. 주사바늘(23)의 길이는 규격화하여 달리 제작할 수 있으며, 제1비코팅부(23-2) 역시 규격화하여 양산할 수 있다. 따라서, 동물 종에 따라 또는 DNA 백신을 주입할 부위에 따라 규격화된 주사바늘(23)로 교체하여 사용하거나, 전기천공용 주사기(20)와 주사바늘(23)을 일체로 규격화하여 양산함으로써 전기천공용 주사기(20) 자체를 교체하여 사용할 수도 있다. DNA 백신 주입시 주사바늘(23)은 동물의 체내로 삽입되며, 주사바늘(23)을 통해 몸체(21)의 DNA 백신이 동물의 체내로 주입된다. 한편, 제1비코팅부(23-2)는 주사바늘(23)을 동물의 체내로 삽입시, 동물의 체내에 위치하도록 구성하는 것이 바람직하다 The entire circumferential surface of the injection needle 23 except for the first non-coated part 23-2 is coated with a non-toxic insulating material to form the first coated part 23-1. As shown in FIG. 4, the first uncoated portion 23-2 may be formed at one lower portion of the injection needle 23, or may be formed at the middle or upper portion of the injection needle 23 although not shown. In addition, as shown in Fig. 5, it may be formed at least in a plurality of positions (the middle part and the lower part), and the formation position is a position to deliver a drug solution (hereinafter referred to as'DNA vaccine') such as a DNA vaccine material. It can be configured in correspondence. That is, since an electric pulse is applied to the first non-coated part 23-2, when the first non-coated part 23-2 is formed at a location to deliver the DNA vaccine, the efficiency of the electroporation (EP) can be improved. I can. The length of the injection needle 23 can be standardized and manufactured differently, and the first uncoated portion 23-2 can also be standardized and mass-produced. Therefore, according to the animal species or the site where the DNA vaccine is to be injected, the standardized injection needle (23) is replaced and used, or the electroporation syringe (20) and the injection needle (23) are integrally standardized and mass produced. The common syringe 20 itself may be replaced and used. When the DNA vaccine is injected, the injection needle 23 is inserted into the body of the animal, and the DNA vaccine of the body 21 is injected into the body of the animal through the injection needle 23. On the other hand, it is preferable that the first non-coated part 23-2 is configured to be located in the body of the animal when the injection needle 23 is inserted into the body of the animal.
또한, 이와 같은 전기천공용 주사기(20)를 이용한 전기천공용 주사 시스템은 전기발생기(10), 전기천공용 주사기(20) 및 부착패드(30)를 포함하여 구성된다. In addition, the electroporation injection system using such an electroporation syringe 20 includes an electric generator 10, an electroporation syringe 20, and an attachment pad 30.
전기발생기(10)는 정전류 또는 정전압을 발생시키며, (+), (-)단자가 형성된다. 즉, 220 볼트 상용전원을 인가받아 일정한 전류(Current constant) 또는 일정한 전압(Voltage constant)을 출력하여 전기펄스(square-wave, 구형파)를 발생시킨다. 한편, 정전압의 경우 전기펄스의 손실로 세포 조직 손상을 유발시킬 수 있을 뿐만 아니라 세포내 DNA 백신의 운반 효율이 저하될 수 있으므로, 전기펄스의 변화가 없는 정전류를 사용하는 것이 바람직하다. The electricity generator 10 generates a constant current or a constant voltage, and (+) and (-) terminals are formed. That is, 220 volt commercial power is applied and a constant current or constant voltage is output to generate an electric pulse (square-wave, square wave). On the other hand, in the case of a constant voltage, it is preferable to use a constant current without a change in the electric pulse, since the loss of the electric pulse may cause damage to the cell tissue as well as the delivery efficiency of the intracellular DNA vaccine may be reduced.
전기천공용 주사기(20)는 DNA(DeoxyriboNucleic Acid) 백신을 동물의 체내에 주입하기 위한 것으로, 일반적인 주사기는 물론, 주사바늘(23) 부위와 DNA 백신이 주입되는 몸체(21)가 의료용 호스 등으로 연결된 형태의 링겔 등을 포함한다. 또한, DNA 백신을 주입할 수 있는 주사바늘(23)이 연결된 형태의 공지된 의료용 물품들을 모두 포함한다 할 것이다.The electroporation syringe 20 is for injecting a DNA (DeoxyriboNucleic Acid) vaccine into the body of an animal. As well as a general syringe, the injection needle 23 and the body 21 to which the DNA vaccine is injected are used with a medical hose, etc. It includes a connected type of ring gel, etc. In addition, it will include all known medical items in the form of a connection with an injection needle 23 capable of injecting a DNA vaccine.
부착패드(30)는 전기발생기(10)의 (+)단자와 (-)단자 중 나머지 다른 하나의 단자와 연결된다. 일례로, 주사바늘(23)에 형성된 커넥터(24)에 케이블(40)을 통해 전기발생기(10)의 (-)단자가 연결되었다면 부착패드(30)에는 전기발생기(10)의 (+)단자가 케이블(40)을 통해 연결된다. 이때, 부착패드(30)는 도 4와 같이 하나로 구성하거나, 도 5와 같이 복수 개로 구성할 수 있다. 각각의 부착패드(30)에는 전기발생기(10)의 (+)단자가 각각의 케이블(40)을 통해 연결된다. 한편, 도 5에 이점쇄선으로 도시한 부분은 동물의 피부(1) 한쪽이 아닌 복수의 위치에서, 예컨대, 동물의 다리 등과 같은 경우 동물의 체내로 삽입된 주사바늘(23)을 기준으로 주사바늘(23)이 삽입된 피부(1)의 반대편에도 부착패드(30)를 부착하여 전기자극을 사방에서 인가하도록 구성할 수도 있다.The attachment pad 30 is connected to the other terminal of the (+) terminal and the (-) terminal of the electric generator 10. For example, if the (-) terminal of the electric generator 10 is connected to the connector 24 formed on the injection needle 23 through the cable 40, the (+) terminal of the electric generator 10 is connected to the attachment pad 30. Is connected through a cable 40. In this case, the attachment pad 30 may be configured as one as shown in FIG. 4 or may be configured as a plurality as shown in FIG. 5. The (+) terminal of the electric generator 10 is connected to each of the attachment pads 30 through respective cables 40. On the other hand, the part shown by the double-dashed line in FIG. 5 is an injection needle based on the injection needle 23 inserted into the body of the animal in the case of a plurality of positions other than one side of the animal's skin 1, for example, the leg of an animal. The attachment pad 30 may also be attached to the opposite side of the skin 1 on which the 23 is inserted, so that electric stimulation may be applied from all directions.
동물의 피부(1)에 부착되는 부착패드(30)의 표면은 도전성 물질로 구성되어 전기발생기(10)의 (+)단자와 연결되는 구조를 갖는다. 또한, 부착패드(30)의 표면에는 동물의 피부(1)에 부착하기 위한 도전성 점착제 등이 형성될 수도 있다.The surface of the attachment pad 30 attached to the animal's skin 1 is made of a conductive material and has a structure that is connected to the (+) terminal of the electric generator 10. In addition, a conductive adhesive for attaching to the skin 1 of an animal may be formed on the surface of the attachment pad 30.
도 4 및 도 5를 참조하면, 복수의 위치에서 한 곳 또는 복수의 위치로 전기자극을 가하게 되므로 종래대비 피부(1)나 조직 손상을 최소화시킬 수 있다.Referring to FIGS. 4 and 5, since electrical stimulation is applied from a plurality of positions to one or a plurality of positions, damage to the skin 1 or tissue can be minimized compared to the prior art.
한편, 미도시 하였으나, 제1비코팅부(23-2)에는 주사바늘(23)의 내부와 연통되는 약액배출공(23-5)이 형성될 수 있다. 약액배출공(23-5)은 제1비코팅부(23-2) 둘레부의 양측에 형성되거나, 사방에 형성될 수 있다. 이와 같이 형성된 약액배출공(23-5)을 통해 DNA 백신이 다수의 위치에서 배출되므로, 전기펄스 자극과 함께 DNA 백신이 세포 내로 잘 전달되도록 유도할 수 있다.Meanwhile, although not shown, a chemical solution discharge hole 23-5 communicating with the inside of the injection needle 23 may be formed in the first uncoated part 23-2. The chemical liquid discharge holes 23-5 may be formed on both sides of the circumference of the first uncoated part 23-2, or may be formed in all directions. Since the DNA vaccine is discharged from a plurality of locations through the drug solution discharge hole 23-5 formed as described above, it is possible to induce the DNA vaccine to be well delivered into the cell with electric pulse stimulation.
도 6은 본 발명인 전기천공용 주사기(20) 및 이를 이용한 전기천공용 주사 시스템의 또 다른 실시례에 따른 구성 개념도이며, 도 7은 도 6의 구성에서 (+)단자를 복수로 구성한 상태를 나타낸 개념도이다. FIG. 6 is a conceptual diagram showing a configuration according to another embodiment of an electroporation syringe 20 and an electroporation injection system using the same, and FIG. 7 shows a state in which a plurality of (+) terminals are configured in the configuration of FIG. It is a conceptual diagram.
도 6과 도 7을 바탕으로 한 전기천공용 주사기(20) 및 이를 이용한 전기천공용 주사 시스템의 기본적인 구조 등은 전술한 도 4와 도 5의 설명을 참조할 수 있으므로, 편의상 전술한 실시례의 구성과 동일한 구성에 대해서는 간략히 기재하도록 한다.The basic structure of the electroporation syringe 20 based on FIGS. 6 and 7 and the electroporation injection system using the same may refer to the descriptions of FIGS. 4 and 5 described above. The same configuration as the configuration will be briefly described.
도 6과 도 7을 참조하면, 본 발명인 전기천공용 주사기(20)는 주사바늘(23)에 전기발생기(10)의 (+), (-) 양 단자가 모두 연결되는 구조를 갖는다.6 and 7, the electroporation syringe 20 according to the present invention has a structure in which both terminals (+) and (-) of the electric generator 10 are connected to the injection needle 23.
즉, 주사바늘(23)에 제1코팅부(23), 제1비코팅부(23-2), 두 개의 단자형성부(24), 제2코팅부(23-3) 및 제2비코팅부(23-4)를 포함한다.That is, the first coated part 23, the first uncoated part 23-2, the two terminal forming parts 24, the second coated part 23-3, and the second non-coated part on the injection needle 23 It includes part (23-4).
먼저, 전기천공용 주사기(20)의 주사바늘(23)에는 전기발생기(10)의 (+), (-) 양 단자 중 어느 하나의 단자와 연결되는 커넥터(24)가 전기적으로 연결된다. First, a connector 24 connected to any one of the positive and negative terminals of the electric generator 10 is electrically connected to the injection needle 23 of the electroporation syringe 20.
제1코팅부(23-1)는, 커넥터(24)의 케이블(40) 연결 부위를 제외한, 주사바늘(23)의 외측 둘레면을 감싸는 무독성 절연물질로 코팅된다. 이때, 동물의 체내로 삽입되는 부위에 무독성 절연물질이 코팅되지 않은 제1비코팅부(23-2)가 적어도 한 곳에 존재한다. 즉, 도 5의 구성과 같이 제1비코팅부(23-2)를 복수로 구성할 수도 있다. 제1비코팅부(23-2)에는 주사바늘(23)의 내부와 연통되는 약액배출공(23-5)이 형성된다.The first coating part 23-1 is coated with a non-toxic insulating material surrounding the outer peripheral surface of the injection needle 23 except for the connection part of the cable 40 of the connector 24. At this time, at least one first non-coated portion 23-2, which is not coated with a non-toxic insulating material, is present at a site inserted into the body of the animal. That is, as in the configuration of FIG. 5, a plurality of first uncoated portions 23-2 may be formed. The first uncoated portion 23-2 has a chemical solution discharge hole 23-5 communicating with the inside of the injection needle 23.
단자형성부(25)는 제1코팅부(23-1)의 외측 둘레면에 형성된다. 단자형성부(25)에도 커넥터(24)가 연결되며, 커넥터(24)를 통해 단자형성부(25)는 전기발생기(10)의 (+), (-)양 단자 중 나머지 다른 하나의 단자와 연결된다. 일례로, 주사바늘(23)에 연결된 커넥터(24)에 (-)단자가 연결되었다면, 단자형성부(25)에 연결된 커넥터(24)에는 (+)단자가 연결된다. 단자형성부(25)는 제1코팅부(23-1)의 둘레면 일부에 형성되며, 제1비코팅부(23-2)와 간섭되지 않는다. 단자형성부(25)는 제1코팅부(23-1)를 감싸는 메탈 재질로 구성되어 커넥터(24)와 연결되거나, 커넥터(24)와 전기적으로 연결된 전선 등으로 구성될 수도 있다. 또한, 주사바늘(23)에 형성된 커넥터(24)는 커플러(22)의 내측에 구성할 수도 있다. 한편, 주사바늘(23)에 연결된 커넥터(24)와 단자형성부(25)에 연결된 커넥터(24) 상호간은 전기적으로 연결되지 않는다. 또한, 도 7에 도시된 바와 같이 전기발생기(10)의 (+)단자는 단자형성부(25)에 연결된 커넥터(24)는 물론, 부착패드(30)를 추가로 구성하여 연결할 수 있다. 또한, 미도시 하였으나, 도 5의 이점쇄선으로 도시된 바와 같이 주사바늘(23)이 삽입된 피부(1) 반대편에도 부착패드(30)를 연결하여 구성할 수 있다. 즉, 다수의 위치에서 한 곳으로 전기자극을 가하도록 구성하여 치료 효과를 높이고, 조직 손상을 감소시킬 수 있다.The terminal forming part 25 is formed on the outer circumferential surface of the first coating part 23-1. The connector 24 is also connected to the terminal forming unit 25, and the terminal forming unit 25 through the connector 24 is connected to the other terminal among the (+) and (-) terminals of the electric generator 10. Connected. For example, if the (-) terminal is connected to the connector 24 connected to the injection needle 23, the (+) terminal is connected to the connector 24 connected to the terminal forming part 25. The terminal forming part 25 is formed on a part of the circumferential surface of the first coated part 23-1, and does not interfere with the first uncoated part 23-2. The terminal forming part 25 may be formed of a metal material surrounding the first coating part 23-1 and may be connected to the connector 24 or may be formed of a wire electrically connected to the connector 24. In addition, the connector 24 formed on the injection needle 23 may be configured inside the coupler 22. Meanwhile, the connector 24 connected to the injection needle 23 and the connector 24 connected to the terminal forming part 25 are not electrically connected to each other. In addition, as shown in FIG. 7, the (+) terminal of the electric generator 10 may be connected to the connector 24 connected to the terminal forming part 25 as well as an attachment pad 30 additionally. In addition, although not shown, the attachment pad 30 may be connected to the opposite side of the skin 1 into which the injection needle 23 is inserted, as shown by the double-dashed line in FIG. 5. That is, it is possible to increase the treatment effect and reduce tissue damage by configuring to apply electrical stimulation from a plurality of locations to one place.
제2코팅부(23-3)는 단자형성부(25)의 외측 둘레면에 무독성 절연물질로 코팅된다. 동물의 체내로 삽입된 단자형성부(25)에는 무독성 절연물질이 코팅되지 않은 제2비코팅부(23-4)가 적어도 한 곳에 존재한다. 한편, 도면상에는 제2비코팅부(23-4)를 단자형성부(25)의 하부에 하나로 구성한 상태를 도시하였다. 또한, 제1비코팅부(23-2)에는 적어도 하나의 홀로 구성된 약액배출공이 형성될 수 있다.The second coating portion 23-3 is coated with a non-toxic insulating material on the outer peripheral surface of the terminal forming portion 25. In the terminal forming part 25 inserted into the body of the animal, at least one second non-coated part 23-4, which is not coated with a non-toxic insulating material, is present. Meanwhile, in the drawing, a state in which the second uncoated portion 23-4 is configured as one under the terminal forming portion 25 is shown. In addition, a chemical liquid discharge hole composed of at least one hole may be formed in the first uncoated part 23-2.
한편, 본 발명에 적용되는 무독성 절연물질은 무독성 실리콘 등과 같은 동물에 무해한 절연재질로 구성하는 것이 바람직하다. 또한, 본 발명에 적용되는 전기발생기(10)는 극성제어기(미도시)와 연결하여 (+)극성과 (-)극성을 변화시켜 사용할 수 있으며, 이는 공지의 기술이므로 이에 대한 자세한 설명은 생략하도록 한다.On the other hand, the non-toxic insulating material applied to the present invention is preferably made of an insulating material harmless to animals such as non-toxic silicone. In addition, the electric generator 10 applied to the present invention can be used by connecting with a polarity controller (not shown) to change the (+) polarity and the (-) polarity. This is a known technique, so a detailed description thereof will be omitted. do.
이상에서 설명한 본 발명인 전기천공용 주사기(20)를 이용한 전기천공용 주사 시스템의 사용 상태를 도 4를 참조하여 설명하면 다음과 같다.The state of use of the injection system for electroporation using the present inventors electroporation syringe 20 described above will be described with reference to FIG. 4.
몸체(21)에 DNA 백신을 주입한다. 주사바늘(23)에 형성된 커넥터(24)와 전기발생기(10)의 (-)단자 사이를 케이블(40)로 연결한다. 부착패드(30)와 전기발생기(10)의 (+)단자 사이를 케이블(40)로 연결한다. 부착패드(30)를 전기천공용 주사기(20)가 삽입된 주변 피부(1)에 부착한다. 전기천공용 주사기(20)의 주사바늘(23)을 동물의 체내로 삽입한다. 전기발생기(10)에 전원을 인가하면 부착패드(30)는 (+)극을 형성하고, 주사바늘(23)의 제1비코팅부(23-2)는 (-)극을 형성하며, 상기 (+)극과 (-)극 사이에 위치한 동물의 피부(1)는 일종의 저항 역할을 하며 전체적으로 폐루프를 형성한다. 따라서, 부착패드(30)로부터 주사바늘(23)의 하부 비코팅부(23-2)로 전기펄스가 인가되면서 주사바늘(23)의 주변 세포막에 일시적으로 통로가 형성된다. 몸체(21)로부터 주사바늘(23)을 통해 동물의 체내로 유입된 DNA 백신은 상기와 같이 일시적으로 형성된 통로를 통해 세포질과 세포핵 안까지 침투한다.DNA vaccine is injected into the body 21. A cable 40 is connected between the connector 24 formed on the injection needle 23 and the (-) terminal of the electric generator 10. Connect between the attachment pad (30) and the (+) terminal of the electric generator (10) with a cable (40). Attach the attachment pad 30 to the surrounding skin 1 into which the electroporation syringe 20 is inserted. The injection needle 23 of the electroporation syringe 20 is inserted into the body of the animal. When power is applied to the electric generator 10, the attachment pad 30 forms a (+) pole, and the first uncoated portion 23-2 of the injection needle 23 forms a (-) pole. The animal's skin (1), located between the (+) and (-) poles, acts as a kind of resistance and forms a closed loop as a whole. Accordingly, as an electric pulse is applied from the attachment pad 30 to the lower uncoated portion 23-2 of the injection needle 23, a passage is temporarily formed in the peripheral cell membrane of the injection needle 23. The DNA vaccine introduced into the body of the animal from the body 21 through the injection needle 23 penetrates into the cytoplasm and the cell nucleus through the passage temporarily formed as described above.
다음으로, 본 발명인 전기천공용 주사기(20)를 이용한 전기천공용 주사 시스템의 다른 사용상태를 도 6을 참조하여 설명하면 다음과 같다.Next, another state of use of the electroporation injection system using the electroporation syringe 20 according to the present invention will be described with reference to FIG. 6.
몸체(21)에 DNA 백신을 주입한다. 주사바늘(23)에 연결된 커넥터(24)와 전기발생기(10)의 (-)단자 사이를 케이블(40)로 연결한다. 단자형성부(24)에 형성된 커넥터(24)와 전기발생기(10)의 (+)단자 사이를 케이블(40)로 연결한다. 의료용 주사기(20)를 동물 체내로 삽입한다. 전기발생기(10)에 전원을 인가하면 단자형성부(25)는 (+)극을 형성하고, 주사바늘(23)의 하부 제1비코팅부(23-2)는 (-)극을 형성하며, 상기 (+)극과 (-)극 사이에 위치한 동물의 피부(1)는 일종의 저항 역할을 하며 전체적으로 폐루프를 형성한다. 따라서, 단자형성부(24)의 하부에 형성된 제2비코팅부(23-4)로부터 주사바늘(23)의 하부에 형성된 제1비코팅부(23-2)로 전기펄스가 인가되면서 주사바늘(23) 주변 세포막에 일시적으로 통로가 형성된다. 몸체(21)로부터 주사바늘(23)을 통해 동물의 체내로 유입된 DNA 백신은 상기와 같이 일시적으로 형성된 통로를 통해 세포질과 세포핵 안까지 침투한다.DNA vaccine is injected into the body 21. Connect between the connector 24 connected to the injection needle 23 and the (-) terminal of the electric generator 10 with a cable 40. A cable 40 is connected between the connector 24 formed on the terminal forming part 24 and the (+) terminal of the electric generator 10. The medical syringe 20 is inserted into the animal body. When power is applied to the electric generator 10, the terminal forming part 25 forms a (+) pole, and the lower first uncoated part 23-2 of the injection needle 23 forms a (-) pole. , The animal skin (1) located between the (+) and (-) poles acts as a kind of resistance and forms a closed loop as a whole. Accordingly, while an electric pulse is applied from the second uncoated portion 23-4 formed under the terminal forming portion 24 to the first uncoated portion 23-2 formed under the injection needle 23, the injection needle (23) A passage is temporarily formed in the surrounding cell membrane. The DNA vaccine introduced into the body of the animal from the body 21 through the injection needle 23 penetrates into the cytoplasm and the cell nucleus through the passage temporarily formed as described above.
상기와 같은 본 발명에 의해 DNA 백신의 주입과 전기펄스 자극을 하나의 주사바늘(23)을 통해 수행할 수 있으므로, 동물에게는 주사바늘(23) 삽입으로 인한 자극을 줄일 수 있다. 또한, 전기천공용 주사기(20)와 이를 이용한 주사 시스템의 전체적인 구성이 간소화되므로 부피를 줄일 수 있고, 사용 편의성을 향상시킬 수 있다. 또한, 제1비코팅부(23-2)의 형성 위치를 DNA 백신을 전달할 위치에 대응시켜 구성함으로써 치료효과를 높임은 물론, DNA 백신 전달 효율을 더욱 향상시킬 수 있다.According to the present invention as described above, since the DNA vaccine injection and electric pulse stimulation can be performed through one injection needle 23, stimulation due to insertion of the injection needle 23 in the animal can be reduced. In addition, since the overall configuration of the electroporation syringe 20 and the injection system using the same is simplified, the volume can be reduced and convenience of use can be improved. In addition, by configuring the formation position of the first non-coating part 23-2 to correspond to the position to which the DNA vaccine is to be delivered, the therapeutic effect can be improved and the DNA vaccine delivery efficiency can be further improved.
이상에서, 본 발명의 실시례를 구성하는 모든 구성 요소들이 하나로 결합하거나 결합하여 동작하는 것으로 설명되었다고 해서, 본 발명이 반드시 이러한 실시례에 한정되는 것은 아니다. 즉, 본 발명의 목적 범위 안에서라면, 그 모든 구성 요소들이 하나 이상으로 선택적으로 결합하여 동작할 수도 있다. 또한, 이상에서 기재된 "포함하다", "구성하다" 또는 "가지다" 등의 용어는, 특별히 반대되는 기재가 없는 한, 해당 구성 요소가 내재할 수 있음을 의미하는 것이므로, 다른 구성 요소를 제외하는 것이 아니라 다른 구성 요소를 더 포함할 수 있는 것으로 해석되어야 한다. 기술적이거나 과학적인 용어를 포함한 모든 용어들은, 다르게 정의되지 않는 한, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 일반적으로 이해되는 것과 동일한 의미가 있다. 사전에 정의된 용어와 같이 일반적으로 사용되는 용어들은 관련 기술의 문맥상의 의미와 일치하는 것으로 해석되어야 하며, 본 발명에서 명백하게 정의하지 않는 한, 이상적이거나 과도하게 형식적인 의미로 해석되지 않는다.In the above, even if all the constituent elements constituting the embodiments of the present invention are described as being combined into one or operating in combination, the present invention is not necessarily limited to these embodiments. That is, within the scope of the object of the present invention, all of the constituent elements may be selectively combined and operated in one or more. In addition, terms such as "include", "consist of" or "have" described above mean that the corresponding component may be present unless otherwise stated, excluding other components Rather, it should be interpreted as being able to further include other components. All terms, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the art, unless otherwise defined. Terms generally used, such as terms defined in the dictionary, should be interpreted as being consistent with the meaning of the context of the related technology, and are not interpreted as ideal or excessively formal meanings unless explicitly defined in the present invention.
이상의 설명은 본 발명의 기술 사상을 예시적으로 설명한 것에 불과한 것으로서, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자라면 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 다양한 수정 및 변형이 가능할 것이다. 따라서, 본 발명에 게시된 실시례들은 본 발명의 기술 사상을 한정하기 위한 것이 아니라 설명하기 위한 것이고, 이러한 실시례에 의하여 본 발명의 기술 사상의 범위가 한정되는 것은 아니다. 본 발명의 보호 범위는 아래의 청구범위에 의하여 해석되어야 하며, 그와 동등한 범위 내에 있는 모든 기술 사상은 본 발명의 권리범위에 포함되는 것으로 해석되어야 할 것이다.The above description is merely illustrative of the technical idea of the present invention, and those of ordinary skill in the art to which the present invention pertains will be able to make various modifications and variations without departing from the essential characteristics of the present invention. Accordingly, the embodiments posted in the present invention are not intended to limit the technical idea of the present invention, but to explain the technical idea, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent thereto should be interpreted as being included in the scope of the present invention.

Claims (13)

  1. 동물에게 사용하며 DNA 백신 물질을 세포 내로 전달하기 위한 전기천공용 주사기에 관한 것으로,It is used in animals and relates to an electroporation syringe for delivering a DNA vaccine material into cells,
    동물의 체내로 들어가는 약액이 유입되는 몸체;A body through which the chemical liquid entering the body of the animal is introduced;
    상기 몸체에 형성되는 커플러;A coupler formed on the body;
    상기 커플러를 통해 상기 몸체와 연결되며, 상기 몸체로부터 약액을 전달받아 동물의 체내로 주입되는 주사바늘; 및An injection needle connected to the body through the coupler and receiving a chemical solution from the body and injected into the body of the animal; And
    상기 커플러와 상기 주사바늘 중 어느 하나에 형성되며, 전기발생기의 (+)단자와 (-)단자 중 적어도 어느 하나의 단자와 연결되는 커넥터;를 포함하는 전기천공용 주사기.An electroporation syringe comprising: a connector formed on one of the coupler and the injection needle and connected to at least one of the (+) terminal and the (-) terminal of the electric generator.
  2. 제1항에 있어서,The method of claim 1,
    동물의 체내로 삽입되는 상기 주사바늘의 측면 둘레부에는,On the side circumference of the injection needle inserted into the body of the animal,
    적어도 하나의 홀로 구성된 약액배출공;이 더 구비되는 전기천공용 주사기.Chemical solution discharge hole consisting of at least one hole; Electroporation syringe further provided.
  3. 제1항에 있어서,The method of claim 1,
    상기 주사바늘의 외측 둘레면에는 무독성 절연물질로 코팅된 제1코팅부가 형성되고, A first coating part coated with a non-toxic insulating material is formed on the outer circumferential surface of the injection needle,
    동물의 체내로 삽입되는 상기 제1코팅부에는 상기 무독성 절연물질이 코팅되지 않은 제1비코팅부가 적어도 한 곳에 형성되며,The first non-coated part, which is not coated with the non-toxic insulating material, is formed on at least one part of the first coated part inserted into the body of the animal,
    상기 제1비코팅부에는 적어도 하나의 홀로 구성된 약액배출공이 더 구비되는 전기천공용 주사기.The first non-coated portion is an electroporation syringe further provided with a chemical solution discharge hole composed of at least one hole.
  4. 제3항에 있어서,The method of claim 3,
    상기 제1코팅부의 외측 둘레면에 결합되며, 도전성 재질로 구성되어 상기 커넥터가 결합되는 단자형성부; 및A terminal forming portion coupled to an outer peripheral surface of the first coating portion and formed of a conductive material to which the connector is coupled; And
    상기 단자형성부의 외측 둘레면에 무독성 절연물질로 코팅된 제2코팅부;를 더 포함하고,Further comprising; a second coating portion coated with a non-toxic insulating material on the outer peripheral surface of the terminal forming portion,
    동물의 체내로 삽입되는 상기 제2코팅부에 상기 무독성 절연물질로 코팅되지 않은 제2비코팅부가 적어도 한 곳에 형성되는 전기천공용 주사기.An electroporation syringe in which a second non-coated part, which is not coated with the non-toxic insulating material, is formed in at least one part of the second coated part inserted into the body of an animal.
  5. 제1항에 있어서,The method of claim 1,
    상기 커플러는,The coupler,
    두 개로 형성되어 커플러 상호간이 절연처리되며,It is formed in two so that the couplers are mutually insulated
    어느 하나의 커플러는 전기발생기의 (+)단자와 연결되고, 나머지 다른 하나의 커플러는 전기발생기의 (-)단자와 연결되며,One coupler is connected to the (+) terminal of the electric generator, and the other coupler is connected to the (-) terminal of the electric generator,
    각각의 상기 커플러에 상기 주사바늘이 결합되는 전기천공용 주사기.An electroporation syringe to which the injection needle is coupled to each of the couplers.
  6. 제5항에 있어서,The method of claim 5,
    동물의 체내로 삽입되는 두 개의 상기 주사바늘 상호간에 마주보는 부위에는 적어도 하나의 홀로 구성된 약액배출공;이 더 구비되는 전기천공용 주사기.An electroporation syringe further provided with a chemical solution discharge hole composed of at least one hole at a portion facing each other between the two injection needles inserted into the body of an animal.
  7. 제5항에 있어서,The method of claim 5,
    각각의 상기 주사바늘의 외측 둘레면에는 무독성 절연물질로 코팅된 제1코팅부가 형성되고, A first coating portion coated with a non-toxic insulating material is formed on the outer circumferential surface of each of the injection needles,
    동물의 체내로 삽입되는 상기 제1코팅부에는 상기 무독성 절연물질이 코팅되지 않은 제1비코팅부가 적어도 한 곳에 형성되며,The first non-coated part, which is not coated with the non-toxic insulating material, is formed on at least one part of the first coated part inserted into the body of the animal,
    상기 제1비코팅부 상호간에 마주보는 부위에는 적어도 하나의 홀로 구성된 약액배출공이 더 구비되는 전기천공용 주사기.An electroporation syringe further provided with a chemical solution discharge hole composed of at least one hole at a portion facing each other of the first non-coated portion.
  8. 제3항에 기재된 전기천공용 주사기를 이용한 전기천공용 주사 시스템에 관한 것으로,It relates to an injection system for electroporation using the electroporation syringe according to claim 3,
    전기발생기;Electric generator;
    동물의 체내로 들어가는 약액이 유입되는 몸체와, 상기 몸체에 형성되는 커플러와, 상기 커플러를 통해 상기 몸체와 연결되며 상기 몸체로부터 약액을 전달받아 동물의 체내로 주입하는 주사바늘과, 상기 커플러와 상기 주사바늘 중 어느 하나에 형성되며 상기 전기발생기의 (+)단자와 (-)단자 중 어느 하나의 단자에 연결되는 커넥터를 포함하는 전기천공용 주사기; 및A body through which a chemical liquid entering the body of an animal flows into the body, a coupler formed in the body, an injection needle connected to the body through the coupler and receiving the chemical liquid from the body and injecting it into the body of the animal, the coupler and the An electroporation syringe formed on any one of the injection needles and including a connector connected to any one of the (+) terminal and the (-) terminal of the electric generator; And
    상기 전기발생기의 (+)단자와 (-)단자 중 나머지 다른 하나의 단자와 연결되어 동물의 피부에 부착되는 적어도 하나의 부착패드;를 포함하며,Including; at least one attachment pad connected to the other terminal of the (+) terminal and the (-) terminal of the electric generator and attached to the skin of the animal,
    상기 전기천공용 주사기는,The electroporation syringe,
    상기 주사바늘의 외측 둘레면에는 무독성 절연물질로 코팅된 제1코팅부가 형성되고, A first coating part coated with a non-toxic insulating material is formed on the outer circumferential surface of the injection needle,
    동물의 체내로 삽입되는 상기 제1코팅부에는 상기 무독성 절연물질이 코팅되지 않은 제1비코팅부가 적어도 한 곳에 형성되는 전기천공용 주사 시스템.An injection system for electroporation in which at least one first non-coated part, which is not coated with the non-toxic insulating material, is formed on the first coated part inserted into the body of an animal.
  9. 제8항에 있어서,The method of claim 8,
    상기 전기천공용 주사기는,The electroporation syringe,
    동물의 체내로 삽입되는 상기 주사바늘의 측면 둘레부에 적어도 하나의 홀로 구성된 약액배출공이 더 구비되는 전기천공용 주사 시스템.An injection system for electroporation, further comprising a chemical solution discharge hole composed of at least one hole at a side circumference of the injection needle inserted into the body of an animal.
  10. 제8항에 있어서,The method of claim 8,
    상기 전기발생기의 (+)극성과 (-)극성 상호간을 변경하여 전달하는 극성제어기를 더 포함하는 전기천공용 주사 시스템.Electroporation injection system further comprising a polarity controller to change and transfer the (+) polarity and (-) polarity of the electric generator.
  11. 제4항에 기재된 전기천공용 주사기를 이용한 전기천공용 주사 시스템에 관한 것으로,It relates to an injection system for electroporation using the electroporation syringe according to claim 4,
    전기발생기; 및Electric generator; And
    동물의 체내로 들어가는 약액이 유입되는 몸체와, 상기 몸체에 형성되는 커플러와, 상기 커플러를 통해 상기 몸체와 연결되며 상기 몸체로부터 약액을 전달받아 동물의 체내로 주입하는 주사바늘과, 상기 커플러와 상기 주사바늘 중 어느 하나에 형성되며 상기 전기발생기의 (+)단자와 (-)단자 중 적어도 어느 하나의 단자에 연결되는 커넥터를 포함하는 전기천공용 주사기;를 포함하며,A body through which a chemical liquid entering the body of an animal flows into the body, a coupler formed in the body, an injection needle connected to the body through the coupler and receiving the chemical liquid from the body and injecting it into the body of the animal, the coupler and the Includes; an electroporation syringe formed on any one of the injection needles and including a connector connected to at least one of the (+) terminal and the (-) terminal of the electric generator,
    상기 전기천공용 주사기는,The electroporation syringe,
    상기 전기발생기의 (+)단자와 (-)단자 중 어느 하나의 단자와 연결되는 상기 주사바늘의 외측 둘레면에는 무독성 절연물질로 코팅된 제1코팅부가 형성되고,A first coating portion coated with a non-toxic insulating material is formed on the outer peripheral surface of the injection needle connected to any one of the (+) terminal and the (-) terminal of the electric generator,
    동물의 체내로 삽입되는 상기 제1코팅부에는 상기 무독성 절연물질이 코팅되지 않은 제1비코팅부가 적어도 한 곳에 형성되며,The first non-coated part, which is not coated with the non-toxic insulating material, is formed on at least one part of the first coated part inserted into the body of the animal,
    상기 제1코팅부의 외측 둘레면에 결합되며, 도전성 재질로 구성되어 상기 전기발생기의 (+)단자와 (-)단자 중 나머지 다른 하나의 단자와 연결되도록 상기 커넥터가 결합되는 단자형성부; 및A terminal forming unit coupled to the outer circumferential surface of the first coating unit and configured of a conductive material to connect the connector to the other of the (+) terminal and the (-) terminal of the electric generator; And
    상기 단자형성부의 외측 둘레면에 무독성 절연물질로 코팅된 제2코팅부;를 더 포함하고,Further comprising; a second coating portion coated with a non-toxic insulating material on the outer peripheral surface of the terminal forming portion,
    동물의 체내로 삽입되는 상기 제2코팅부에는 상기 무독성 절연물질로 코팅되지 않은 제2비코팅부가 적어도 한 곳에 형성되는 전기천공용 주사 시스템.An injection system for electroporation in which at least one second non-coated part not coated with the non-toxic insulating material is formed on the second coated part inserted into the body of an animal.
  12. 제11항에 있어서,The method of claim 11,
    상기 전기발생기의 (+)단자와 (-)단자 중 어느 하나의 단자와 연결되어 동물의 피부에 부착되는 적어도 하나의 부착패드;를 더 포함하는 전기천공용 주사 시스템.Electroporation injection system further comprising: at least one attachment pad connected to any one of the (+) terminal and the (-) terminal of the electric generator and attached to the skin of the animal.
  13. 제11항에 있어서,The method of claim 11,
    상기 전기발생기의 (+)극성과 (-)극성 상호간을 변경하여 전달하는 극성제어기를 더 포함하는 전기천공용 주사 시스템.Electroporation injection system further comprising a polarity controller to change and transfer the (+) polarity and (-) polarity of the electric generator.
PCT/KR2020/007765 2019-06-18 2020-06-16 Electroporation syringe and electroporation injection system using same WO2020256375A2 (en)

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KR20190072268 2019-06-18
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KR1020200067644A KR102470989B1 (en) 2019-06-18 2020-06-04 Syringe for electroporation and syringe system for electroporation using the same

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US6678556B1 (en) * 1998-07-13 2004-01-13 Genetronics, Inc. Electrical field therapy with reduced histopathological change in muscle
US6314317B1 (en) * 1999-02-18 2001-11-06 Biovalve Technologies, Inc. Electroactive pore
US7328064B2 (en) * 2002-07-04 2008-02-05 Inovio As Electroporation device and injection apparatus
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