WO2020227855A1 - 样本检测装置和样本检测装置的运行方法 - Google Patents

样本检测装置和样本检测装置的运行方法 Download PDF

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Publication number
WO2020227855A1
WO2020227855A1 PCT/CN2019/086432 CN2019086432W WO2020227855A1 WO 2020227855 A1 WO2020227855 A1 WO 2020227855A1 CN 2019086432 W CN2019086432 W CN 2019086432W WO 2020227855 A1 WO2020227855 A1 WO 2020227855A1
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WIPO (PCT)
Prior art keywords
pipeline
reagent
sample
access point
detection device
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PCT/CN2019/086432
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English (en)
French (fr)
Inventor
刘隐明
滕锦
石汇林
向凤光
Original Assignee
深圳迈瑞生物医疗电子股份有限公司
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Application filed by 深圳迈瑞生物医疗电子股份有限公司 filed Critical 深圳迈瑞生物医疗电子股份有限公司
Priority to CN201980095492.8A priority Critical patent/CN113711049A/zh
Priority to PCT/CN2019/086432 priority patent/WO2020227855A1/zh
Publication of WO2020227855A1 publication Critical patent/WO2020227855A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system

Definitions

  • the present invention relates to the technical field of medical devices, in particular to a sample detection device and an operating method of the sample detection device.
  • Fluorescent reagents are reagents used in high-end hemocytometer products. Fluorescent dyes are inserted into the DNA groove to change the structural freedom and electronic state of the dye molecules, thereby changing the fluorescence characteristics of the molecules and enhancing the fluorescence.
  • the cells are illuminated by laser, and the cell structure is different. Different light is emitted, the signal is received by the receiver, and different cell classifications are obtained after processing.
  • the application provides a sample detection device and an operating method of the sample detection device.
  • an embodiment of the present application provides a sample detection device, including:
  • a first reagent component including a first reagent container and a first pipeline for accommodating the first reagent;
  • a second reagent component comprising a second reagent container for accommodating a second reagent and a second pipeline, the second reagent being different from the first reagent;
  • a reaction cell for processing the biological sample to form the sample to be tested, the reaction cell communicating with the first pipeline and the second pipeline;
  • a spacer is arranged at least on the first pipeline, and the spacer is used to reduce the contact between the first reagent and the reagent in the reaction tank and/or the second reagent
  • an embodiment of the present application also provides an operating method of a sample detection device, which is applied to a sample detection device.
  • the sample detection device includes a first pipeline, a second pipeline, and a reaction cell. And the second pipeline are connected to the reaction tank, and the method includes:
  • the sample detection device provided by the embodiment of the present application can reduce the contact of the first reagent, the reagent in the reaction tank, and/or the second reagent by arranging the spacer on at least the first pipeline connected to the first reagent container, thereby reducing The risk of the first reagent being contaminated by the reagent in the reaction tank and/or the second reagent reduces the consumption of the first reagent.
  • Figure 1 is a schematic diagram of a sample detection device provided by an embodiment of the present application.
  • FIG. 2 is a schematic flowchart of a sample detection method of the sample detection device shown in FIG. 1;
  • Fig. 3 is a schematic diagram of a reaction component in an embodiment of the sample detection device shown in Fig. 1;
  • Fig. 4 is a schematic diagram of the spacer in the reaction assembly shown in Fig. 3;
  • Fig. 5 is a schematic view from another angle of the spacer in the reaction assembly shown in 3;
  • Fig. 6 is a schematic diagram of a reaction component in another embodiment of the sample detection device shown in Fig. 1;
  • FIG. 7 is a schematic flowchart of a sample detection method of the reaction assembly shown in FIG. 6;
  • FIG. 8 is a schematic flowchart of another sample detection method of the sample detection device shown in FIG. 6;
  • Fig. 9 is a schematic diagram of a reaction component in another embodiment of the sample detection device shown in Fig. 6;
  • FIG. 10 is a schematic flowchart of the sample detection method of the reaction assembly shown in FIG. 9;
  • Fig. 11 is a schematic diagram of a reaction component in another embodiment of the sample detection device shown in Fig. 6;
  • Fig. 12 is a schematic diagram of a reaction component in another embodiment of the sample detection device shown in Fig. 1;
  • FIG. 13 is a schematic flowchart of a sample detection method of the reaction assembly shown in FIG. 12;
  • Fig. 14 is a schematic diagram of a reaction component in another embodiment of the sample detection device shown in Fig. 12;
  • FIG. 15 is a schematic diagram of a reaction component in another embodiment of the sample detection device shown in FIG. 12;
  • Fig. 16 is a schematic diagram of a reaction component in another embodiment of the sample detection device shown in Fig. 12.
  • a sample detection device 100 is provided for this embodiment of the application, and the sample detection device 100 is used to detect samples.
  • the reaction cell 3 in the sample detection device 100 is used to process the sample to form a sample to be tested, and the sample to be tested can be transported to the detection assembly 5 for measurement.
  • the specific types of samples are not limited.
  • the sample includes a fixed sample or a liquid sample. Further liquid samples include but are not limited to blood samples.
  • the manner in which the reaction tank 3 processes the sample to form the sample to be tested includes but is not limited to the following manner: the sample is incubated with various required reagents to form the sample to be tested.
  • the names of the various stages of the sample are described in detail here; the sample is defined as the sample to be reacted before the incubation reaction in the reaction tank 3, and the sample to be reacted is in the reaction tank 3.
  • the reactant formed after the reagents complete the incubation reaction is defined as the sample to be tested.
  • the content of the reaction tank 3 is equipped with a diluent bottom liquid.
  • the diluent bottom liquid in the reaction tank 3 needs to be emptied to prevent dilution
  • the liquid bottom solution dilutes the sample to be reacted or the reagent that reacts with the sample to be reacted.
  • the diluent bottom liquid in the reaction tank 3 generally comes into contact with the reagent to be incubated with the sample to be reacted for a long time, thereby
  • the reagent is diluted, in other words, the reagent will be contaminated by the diluent base solution, and the contaminated reagent needs to be drained to avoid abnormal test results of the sample to be tested, resulting in a large consumption of the reagent.
  • the sample detection device 100 includes a first reagent component 1, a second reagent component 2, a reaction cell 3, and an isolation Item 4,
  • the first reagent assembly 1 includes a first reagent container 11 for accommodating a first reagent and a first pipeline 12;
  • the second reagent assembly 2 includes a second reagent container 21 for accommodating a second reagent and a first Two pipelines 22, the second reagent is different from the first reagent;
  • the reaction cell 3 is used to process the biological sample to form the sample to be tested, and the reaction cell 3 communicates with the first pipeline 12 and the second pipeline 22;
  • the partition 4 At least on the first pipeline 12, the spacer 4 is used to reduce the contact between the first reagent and the reagent in the reaction tank 3 and/or the second reagent.
  • the first reagent in the first reagent component 1 and the second reagent in the second reagent component 2 can be incubated and reacted with the sample to be reacted in the reaction tank 3 to form the sample to be tested.
  • the first reagent includes but is not limited to fluorescent liquid.
  • the second reagent includes but is not limited to hemolysis or other reagent solutions.
  • the reaction tank 3 is connected to the first pipeline 12 and the second pipeline 22, which can be understood as the first reagent in the first pipeline 12 can flow into the reaction tank 3, and the second reagent in the second pipeline 22 can flow into the reaction tank. 3.
  • the spacer 4 is used to reduce the contact between the first reagent and the reagent in the reaction tank 3 and/or the second reagent.
  • the spacer 4 can be used to reduce the contact between the first reagent and the reagent in the reaction tank 3, and isolate
  • the element 4 can be used to reduce the contact between the first reagent and the second reagent, and the spacer 4 can also be used to reduce the contact between the first reagent and the reagent in the reaction tank 3 and the second reagent.
  • the spacer 4 By arranging the spacer 4 on at least the first pipeline 12, the contact of the first reagent, such as fluorescent reagent, of the first pipeline 12 assembly with the diluent of the reaction cell 3 is reduced, or the amount of the first pipeline 12 assembly is reduced.
  • the contact of the first reagent, such as the fluorescent reagent, with the second reagent of the second pipe 22 assembly, or the contact of the fluorescent reagent with the diluent of the reaction cell 3 and the reagent of the second pipe 22 assembly is reduced, thereby reducing the first pipe The consumption of the first reagent of the circuit 12 component.
  • the sample detection device 100 provided by this embodiment of the present application, the first pipeline 12 and the second pipeline 22 in the sample detection device 100 communicate with the reaction cell 3 through the spacer 4.
  • the first pipeline 12 has a first access point S1
  • the second pipeline 22 has a second access point S2.
  • the sample detection device 100 further includes a third pipeline 31 connected to the reaction cell 3.
  • the third pipeline 31 has a third access point S3, and the first access point S1 and the second access point S2 communicate with the third access point S3 via the partition 4.
  • the partition 4 is provided on the first pipeline 12 and the second pipeline 22 at the same time, and the first pipeline 12 and the second pipeline 22 communicate with the reaction cell 3 through the partition 4.
  • the first reagent in the first pipeline 12 and the second reagent in the second pipeline 22 reduce the contact with the diluted bottom liquid in the reaction cell 3 through the spacer 4, thereby reducing the first pipeline 12 assembly.
  • the first reagent assembly 1, the second reagent assembly 2, the reaction tank 3 and the spacer 4 are mainly used for incubation reaction, so for the convenience of description, the above-mentioned components are collectively referred to as the reaction assembly 10.
  • the first reagent in the first reagent component 1 and the first reagent in the second reagent component 2 are completely separated from the reagent in the third pipeline 31 by the spacer 4 .
  • the spacer 4 includes a first valve body and a first valve core located in the first valve body.
  • the first valve body has two first inlets and a first outlet.
  • An access point S1 and a second access point S2 are respectively connected to a first access port of the spacer 4, and a third access point S3 is connected to the first port of the spacer 4; the first valve core is opposite to the first valve Physical activity to connect the first access point S1 or the second access point S2 with the third access point S3.
  • the third access point S3 can be connected with the first access point S1, or the third access point S3 can be connected with the second access point S2 by controlling the energization of the first valve body and the first valve core.
  • the third access point S3 is connected to the second access point S2, in other words, when the first valve body and the first valve core are in the power-off state ,
  • the second reagent in the second pipeline 22 can flow into the reaction cell 3, while the first reagent in the first pipeline 12 cannot flow into the reaction cell 3 because it is isolated by the partition 4, in other words, the reagent in the reaction cell 3 is also Can not diffuse into the first pipe 12 and pollute the first reagent in the first pipe 12, thereby completely isolating the first reagent in the first pipe 12 from the reaction tank 3; the first valve body and the first valve core
  • the second reagent in the first pipeline 12 can flow into the reaction cell 3, but the second reagent in the second pipeline 22 cannot flow into the reaction cell 3 because it is isolated by the partition 4.
  • FIG. 2 is a flow chart of the operation method of the sample detection device 100 shown in FIG. 1, and the operation method of the sample detection device 100 includes: 101: Emptying the diluent bottom liquid in the reaction tank 3. Among them, the diluent bottom liquid is drained to prevent the diluent bottom liquid from affecting the subsequent incubation reaction.
  • the second reagent in the second reagent container 21 flows into the reaction through the second channel 22, the second access point S2 and the third access point S3 in sequence for the first time
  • the second reagent is drained from the pool 3 side by side.
  • This step is to prevent the diluent bottom liquid or pollutants still remaining in the reaction tank 3 from affecting the subsequent incubation reaction.
  • the first valve core is in a power-off state, in other words, the second access point S2 and the third access point S3 are in a conducting state to form a second channel.
  • the second reagent in the second reagent container 21 flows into the reaction through the second pipeline 22, the second channel formed by the second access point S2 and the third access point S3, and the third pipeline 31 for the second time. Inside the pool 3.
  • the second reagent is used to perform an incubation reaction with the subsequent sample to be reacted and the first reagent to form the sample to be tested.
  • the first reagent is used to react with the second reagent contained in the reaction tank 3 and the subsequent sample to be reacted to form the sample to be tested.
  • the sample to be reacted is incubated with the first reagent and the second reagent to form the sample to be tested.
  • the second reagent in the operating method of the sample detection device 100 can also be added only once, in other words step 103 and step 105 can be omitted. It can be understood that the sequence in which the first reagent, the second reagent, and the sample to be reacted flow into the reaction cell in the operating method of the sample detection device 100 shown in FIG. 2 is an optional embodiment. The order of adding the first reagent, the second reagent and the sample to be reacted can be changed. For example, the first reagent, the second reagent, and the sample to be reacted can be added at the same time or sequentially.
  • the sample to be reacted is a blood sample.
  • the bottom liquid may be a diluent.
  • the spacer 4 is configured to include a first valve body and a first valve core, so that the first reagent is only connected when the first reagent needs to be added to the reaction cell 3.
  • a pipeline 12 and the reaction cell 3 so that the diluent in the reaction cell 3 and the second reagent in the second pipeline 22 are completely isolated by the partition 4 without being diffused into the first pipeline 12, so that the first The first reagent in a reagent container 11 will not be contaminated by the diluent in the reaction tank 3 and the second reagent in the second pipeline 22, thereby reducing the consumption of the first reagent.
  • FIG. 3 is a schematic diagram of the reaction assembly 10 of the sample detection assembly 5 provided in FIG. 1.
  • the spacer 4 can also have the following structure to realize the first reagent and the first reagent in the first reagent assembly 1
  • the second reagent in the second reagent assembly 2 is completely isolated from the reagent in the third pipeline 31 by the spacer 4.
  • FIGS. 4 and 5 are schematic diagrams of the spacer 4 in the reaction assembly 10 shown in FIG. 3.
  • the isolator 4 includes a second valve body 43 and a second valve core 44 located in the second valve body 43.
  • the second valve body 43 has two second inlets 43a and a second outlet 43b.
  • the first access point S1 and the second access point S2 are respectively connected to a second access port 43a of the spacer 4, and the third access point S3 is connected to the second port 43b of the spacer 4; when the second valve core 44 is located in the second valve When the body 43 is in the first position, the connection between the first access point S1 and the third access point S3 is disconnected, and the second access point S2 is connected to the third access point S3; when the second spool 44 is in the second When the valve body 43 is in the second position, the first access point S1 is in communication with the third access point S3, and the second access point S2 is in communication with the third access point S3.
  • two second inlets 43a and one second inlet 43b are located on the same side of the second valve body 43, so that the pipeline can be connected to the second valve body 43 easily.
  • the second inlet 43a for accessing the first pipeline 12 is located at the second inlet 43a for accessing the second pipeline 22 and the second inlet 43b for accessing the third pipeline 31 between.
  • the second valve body 43 blocks the second inlet 43a for accessing the first pipeline 12, and correspondingly, the second inlet for accessing the second pipeline 22 43a is connected to the second interface, so that the second reagent in the second pipeline 22 can flow into the reaction cell 3; when the isolator 4 is in the energized state, the second valve body 43 moves away from the first tube The direction movement of the second inlet 43a of the circuit 12 is used to connect the second inlet 43a of the first pipeline 12 to the second outlet 43b, so that the first reagent in the first pipeline 12 can flow in Reaction cell 3.
  • the spacer 4 can also incompletely isolate the first reagent from other reagents, thereby reducing the contact between the first reagent and other reagents, and reducing the amount in the first pipeline 12.
  • the consumption of the first reagent Specifically, as shown in FIG. 6, the spacer 4 has a joint 45.
  • the joint 45 has an inner cavity and three interfaces communicating with the inner cavity, a first access point S1, a second access point S2, and a third access point. S3 is connected to an interface of the connector 45 respectively.
  • the inner diameter of the interface where the connector 45 enters the first access point S1 is 0.1 mm to 0.35 mm.
  • the connector 45 may be a three-way connector 45.
  • the connector 45 may be a three-way connector 45.
  • FIG. 7 is a flowchart of the operation method of the sample detection device 100 shown in FIG. 6.
  • the operation method of the sample detection device 100 includes:
  • the second reagent in the second reagent container 21 flows into the reaction cell 3 through the second pipe 22, the connector 45, and the third pipe 31 in sequence for the first time, and the second reagent is discharged to prevent the reaction tank 3
  • the remaining diluent substrate will affect the subsequent incubation reaction.
  • the second reagent is used to perform an incubation reaction with the subsequent sample to be reacted and the first reagent to form the sample to be tested.
  • the first reagent is used for an incubation reaction with the second reagent contained in the reaction tank 3 and the subsequent sample to be reacted to form the sample to be tested.
  • the second reagent in the second reagent container 21 flows into the reaction cell 3 through the second pipe 22, the joint 45, and the third pipe 31 for the third time, and at the same time, the sample to be reacted is added to the reaction tank 3.
  • the sample to be reacted is incubated with the first reagent and the second reagent to form the sample to be tested.
  • the second reagent in the operating method of the sample detection device 100 can also be added only once, in other words step 203 and step 205 can be omitted. It can be understood that the sequence of adding the first reagent, the second reagent, and the sample to be reacted in the operating method of the sample detection device 100 shown in FIG. 7 is an optional embodiment, the first reagent, the second reagent The order of adding the sample to be reacted can be changed. For example, the first reagent, the second reagent, and the sample to be reacted can be added at the same time, or added sequentially, and so on.
  • the spacer 4 is configured as a joint 45, which communicates with the first pipeline 12, the second pipeline 22, and the third pipeline 31, respectively, and the joint 45 is connected to the third pipeline.
  • the inner diameter of the interface connected with a pipeline 12 is small, so as to better reduce the diffusion of the reagent in the second pipeline 22 or the third pipeline 31 into the first pipeline 12, thereby reducing the first pipeline 12. The consumption of the first reagent.
  • the spacer 4 further includes a first air column, the first air column is located in the first pipeline 12 and is close to the first reagent container 11.
  • the first air column is formed of air.
  • FIG. 8 is a flowchart of the operation method of the sample detection device 100 shown in FIG. 6.
  • the operation method of the sample detection device 100 runs after the reaction tank 3 forms a side sample, and the reaction tank 3 is cleaned and emptied. After the steps, in other words, the method includes:
  • the sample detection device 100 needs to form the next sample to be tested in the reaction cell 3 to perform a sample test, please refer to steps 201 to 213 for the operation method flow of the sample detection device 100, which will not be repeated here.
  • the first gas column is eliminated during the inflow process.
  • the pipe formed with the first gas column that is, the first pipe 12
  • the first air column will also enter the second pipeline 22.
  • the first air column may also be located in the second pipeline 22.
  • step 301 can correspond to the first pipeline 12 and the second pipeline 22 to suck back air to form a first air column, then there is When passing the first air column for the first time, the first air column will be broken.
  • the first air column may also be located only in the second pipeline 22.
  • the spacer 4 is configured as a joint 45 and a first gas column located in the first pipeline 12 and/or the second pipeline 22.
  • the first gas column When the first gas column is located in the pipeline It can effectively prevent other reagents from spreading into the pipeline, thereby preventing the reagent in the pipeline from being contaminated, thereby reducing the consumption of the reagent in the pipeline.
  • the isolating member 4 further has a two-way valve 46.
  • the two-way valve 46 is provided on the first pipeline 12 and is located between the first reagent container 11 and the first access point S1. between.
  • the internal volume of the two-way valve 46 is small, and the two-way valve 46 is close to the joint 45.
  • the two-way valve 46 can effectively prevent other reagents from flowing into the first reagent container 11 through the connector 45, thereby reducing the consumption of the first reagent.
  • FIG. 10 is a flowchart of the operation method of the sample detection device 100 shown in FIG. 9.
  • the operation method of the sample detection device 100 includes: 401: emptying the diluent bottom liquid in the reaction tank 3. Among them, the diluent bottom liquid is drained to prevent the diluent bottom liquid from affecting the subsequent incubation reaction.
  • the second reagent in the second reagent container 21 flows into the reaction cell 3 through the second pipe 22, the connector 45, and the third pipe 31 in sequence for the first time, and the second reagent is discharged to prevent the reaction tank 3
  • the remaining diluent substrate will affect the subsequent incubation reaction.
  • the second reagent in the second reagent container 21 flows into the reaction cell 3 through the second pipe 22, the connector 45, and the third pipe 31 for the third time, and at the same time, the sample to be reacted is added to the reaction tank 3.
  • the sample to be reacted is incubated with the first reagent and the second reagent to form the sample to be tested.
  • the isolator 4 further has a check valve 47.
  • the check valve 47 is provided on the first pipeline 12 and located between the first reagent container 11 and the first access point S1. between.
  • the interface of the connector 45 connected to the first access point S1 is connected to the outlet of the one-way valve 47.
  • the joint 45 and the check valve 47 are integrated.
  • the spacer 4 of this structure enables the first reagent in the first pipeline 12 to flow into the reaction cell 3 from the spacer 4, and the corresponding check valve 47 makes the second reagent in the second pipeline 22 and the reaction cell
  • the reagent in 3 cannot flow through the first reagent container 11 through the one-way valve 47, which prevents the first reagent from being contaminated by other reagents, thereby reducing the consumption of the first reagent.
  • the reaction process of the sample detection device 100 is roughly the same as steps 401 to 413, except that the first reagent in the first reagent container 11 is passed through the first pipeline 12, the one-way valve 47, the connector 45, and the third The pipe 31 flows into the reaction cell 3.
  • the first reagent is used for an incubation reaction with the second reagent contained in the reaction tank 3 and the subsequent sample to be reacted to form the sample to be tested.
  • the reaction assembly 10 As shown in Figures 12 to 17, the reaction assembly 10 provided by this embodiment of the application, the reaction assembly 10 completely isolates the first pipeline by connecting the first pipeline 12 and the second pipeline 22 to the reaction tank 3 respectively Mutual contamination between the first reagent in 12 and the second reagent in the second pipeline 22, and the spacer 4 is only provided on the first pipeline 12 to reduce the first reagent in the first pipeline 12 and the reagent in the reaction cell 3 , Thereby reducing the consumption of the first reagent in the first pipeline 12. Specifically, the first pipeline 12 and the second pipeline 22 are respectively connected to the reaction tank 3, and the spacer 4 is provided on the first pipeline 12.
  • a partition 4 is formed at a section of the first pipeline 12 connected to the reaction tank 3.
  • the inner diameter of the partition 4 is the minimum inner diameter of the first pipeline 12, and the inner diameter ranges from 0.1 mm to 0.35. mm.
  • the spacer 4 may be a part of the first pipeline 12, and the spacer 4 may also be a steel pipe sleeved at the end of the first pipeline 12, and the inner diameter of the steel pipe is 0.1 mm to 0.35 mm.
  • the diffusion speed of the reagent in the reaction tank 3 to the first pipeline 12 is reduced, thereby reducing the first pipeline
  • FIG. 13 is a flowchart of the operation method of the sample detection device 100 shown in FIG. 12.
  • the operation method of the sample detection device 100 includes:
  • the second reagent in the second reagent container 21 flows into the reaction tank 3 through the second pipe 22 for the first time and the second reagent is discharged, so as to prevent the remaining diluent in the reaction tank 3 from affecting the subsequent The incubation reaction has an impact.
  • the first reagent is used for an incubation reaction with the second reagent contained in the reaction tank 3 and the subsequent sample to be reacted to form the sample to be tested.
  • the spacer 4 is a second air column
  • the second air column is located in the first pipe 12 for blocking the communication between the first reagent and the reaction cell 3.
  • the first pipeline 12 and the second pipeline 12 respectively communicate with the reaction cell 3, and the first pipeline 12 sucks air back to form a second air column.
  • the second air column is formed of air.
  • the second air column can effectively prevent other reagents from spreading into the first pipe 12, thereby preventing the reagents in the first pipe 12 from being Pollution, thereby reducing the consumption of reagents in the first pipeline 12.
  • the second air column here may be the same as the aforementioned first air column.
  • the operating method of the sample detection device 100 runs after the step of washing and emptying the reaction tank 3 after the reaction tank 3 forms a sample to be tested.
  • steps 301 to 305 which will not be repeated here.
  • the sample detection device 100 needs to form the next sample to be tested in the reaction cell 3, please refer to steps 501 to 513 for the operation method flow of the sample detection device 100, which will not be repeated here.
  • the pipeline formed with the first air column that is, the first pipeline 12 will be broken when the first reagent passes.
  • the spacer 4 is a two-way valve or a one-way valve connected in series in the first pipeline 12.
  • the spacer 4 may be a two-way valve connected in series in the first pipeline 12, the internal volume of the two-way valve is small, and the two-way valve is close to the joint 45.
  • the two-way valve can effectively prevent the reagent in the reaction tank 3 from flowing into the first reagent container 11, thereby reducing the consumption of the first reagent.
  • the spacer 4 may be a one-way valve connected in series in the first pipeline 12.
  • the spacer 4 of this structure enables the first reagent in the first pipeline 12 to pass from the spacer 4 It flows into the reaction tank 3, and the corresponding check valve prevents the reagents in the reaction tank 3 from flowing through the first reagent container 11 through the check valve, which prevents the first reagent from being contaminated by other reagents, thereby reducing the amount of the first reagent. consumption.
  • the reaction process of the sample detection device 100 is roughly the same as steps 501 to 513, and the difference is that the first reagent in the first reagent container 11 flows into the reaction tank through the first pipeline 12, a two-way valve or a one-way valve 3 within.
  • the second pipeline 22 is connected to the reaction cell 3, and the first pipeline 12 is connected to the reaction cell 3 through the spacer 4, and the spacer 4 is a sleeve and is suspended in the reaction cell. 3 on.
  • the first pipeline 12 is suspended on the reaction cell 3 to reduce the rate of mutual diffusion between the first pipeline 12 and the reaction cell 3, thereby reducing the consumption of the first reagent in the first pipeline 12.
  • the sample detection device 100 further includes a main circuit 6 and a detection component 5.
  • the main circuit 6 has a fourth access point S4, and the reaction cell 3 is connected to the fourth access point S4 of the main circuit 6.
  • the detection component 5 includes a sample needle 51, the sample needle 51 has a fifth access point S5, the sample to be tested in the reaction cell 3 flows through the fourth access point S4 of the main line 6 to the fifth access point of the sample needle 51 Point S5, so that the sample to be tested in the reaction cell 3 flows into the sample in the detection assembly 5 for detection.
  • the sample detection device 100 further includes a fourth pipe 32 connected between the reaction cell 3 and the main pipe 6, and a first switching element 33 provided on the fourth pipe 32, wherein the first switching element 33 is located Between the fourth access point S4 and the main line 6. If the first switching element 33 is turned on, that is, when the first switching element 33 realizes the communication between the reaction cell 3 and the main line 6, the sample to be tested that has completed the reaction in the reaction cell 3 can pass through the fourth line 32 and the fourth access point in sequence. S4 flows to the fifth access point S5 of the sample needle 51 to enter the detection assembly 5 for detection. If the first switching element 33 is closed, that is, when the first switching element 33 cuts off the inner fourth pipeline 32, the liquid to be tested in the fourth pipeline 32 cannot flow into the main pipeline 6.
  • the sample detection device 100 further includes a fifth pipeline 61 connected to the fourth access point S4 and a first diluent tank 62 connected to the fifth pipeline 61.
  • the diluent in the first diluent tank 62 can flow into the reaction tank 3 through the fifth pipeline 61, the fourth access point S4 and the fourth pipeline 32.
  • the sample detection device 100 further includes a drainage tank 34 connected to the reaction tank 3.
  • the liquid drain tank 34 can be used to store the liquid discharged from the reaction tank 3.
  • the detection assembly 5 further includes a flow chamber 52, a sheath liquid tank 53 and a first waste liquid tank 54.
  • the flow chamber 52 has an inlet for the sample to be tested, an inlet and an outlet for the sheath fluid.
  • the inlet for the sample to be tested is connected to the fifth access point S5 of the sample needle 51, and the sheath fluid inlet is connected to the sheath fluid pool 53, which is used to store the sheath fluid.
  • the outlet is used to connect the first waste liquid tank 54.
  • the sheath liquid tank 53 is connected to the first gas storage tank. When the two are connected, the first positive pressure provided by the first gas storage tank pushes the sheath liquid to flow into the flow chamber 52.
  • the sample to be tested enters the flow chamber 52 from the sample to be tested inlet, the sheath fluid in the sheath liquid pool 53 can flow into the flow chamber 52 through the sheath fluid inlet, and the sheath fluid wraps the sample to be tested to form the sample to be tested
  • the sample stream is tested, and the tested sample stream enters the first waste liquid pool 54 from the outlet.
  • the sample detection device 100 further includes a sample pushing component 7, which pushes the sample to be tested in the sample preparation section to be tested into the sample needle 51 of the detection component 5 by setting the sample pushing component 7.
  • the pushing components 7 are respectively connected to the sixth access point S6 of the main road 6, the sixth access point S6 is located between the fourth access point S4 and the fifth access point S5, and the sixth access point S6 and the fifth access point S6
  • the channel between the access points S5 is the sample preparation section; the sample to be tested in the reaction pool 3 flows through the fourth access point S4 of the main line 6 to the sixth access point S6 of the main line 6, and passes through the supervisor
  • the sixth access point S6 of the road 6 flows into the sample preparation section to be tested; the sample pushing component 7 is used to push the sample to be tested in the sample preparation section to be tested into the sample needle 51 of the detection component 5.
  • the sample pushing assembly 7 includes a syringe 71, a sixth pipeline 72, a second switch 73 provided on the sixth pipeline 72, and a second diluent tank 74.
  • the second switching element 73 When the second switching element 73 is turned on, the diluent in the second diluent pool 74 can flow into the syringe 71; the liquid in the syringe 71 is pushed from the sixth access point S6 to the fifth access point S5, so as to align the sample to be tested.
  • the sample to be tested in this paragraph is pushed into the fifth access point S5 for detection by the detection component 5.
  • the sixth pipeline 72 is cut off.
  • the sample detection device 100 further includes a third switching element 63 provided on the main pipe 6, and the third switching element 63 is located between the sixth access point S6 and the fourth access point S4.
  • the third switch 63 When the third switch 63 is turned on, the fourth access point S4 is connected to the sample preparation section to be tested.
  • the third switch 63 When the third switch 63 is closed, the main line 6 is cut off.
  • the sample detection device 100 further includes a sampling component 8, which is connected to the end of the main pipe 6, and the sampling component 8 is used to form a negative pressure to extract the sample to be tested in the reaction tank 3 to the sample preparation section for testing. Inside.
  • the sampling assembly 8 includes a second waste liquid tank 81, and a negative pressure is formed in the second waste liquid tank 81, and the negative pressure draws the sample to be tested in the reaction tank 3 into the sample preparation section for the test.
  • an incubation reaction is first performed in the reaction tank 3 (for the specific reaction process of the reaction component 10, please refer to the reaction process mentioned in the foregoing embodiment, which will not be repeated here). After the incubation reaction is completed The sample to be side flows into the detection assembly 5 for detection.
  • the flow path of the sample to be tested is: making the sampling assembly 8 generate negative pressure, turning on the first switching element 33 and the third switching element 63, so that the sample to be tested flows from the reaction cell 3 through the fourth pipeline 32, The fourth access point S4, the main line 4 until it flows into the sample preparation section to be tested, the second switch 73 is turned on, and the diluent in the second diluent pool 74 flows into the syringe 71; the liquid in the syringe 71 is connected from the sixth Point S6 is pushed into the fifth access point S5 to push the sample to be tested in this section of the sample to be tested into the fifth access point S5 for detection by the detection component 5; when the detection component 5 performs detection, the sample to be tested Enter the flow chamber 52 from the inlet of the sample to be tested, the sheath fluid in the sheath fluid pool 53 can flow into the flow chamber 52 through the sheath fluid inlet, and the sheath fluid wraps the sample to be tested to form a sample stream
  • the sample detection device 100 reduces the contact of the first reagent, such as fluorescent reagent, of the first pipeline 12 component with the diluent of the reaction cell 3, by arranging the spacer 4 on at least the first pipeline 12. Or reduce the contact of the first reagent of the first tube 12 component, such as the fluorescent reagent with the second reagent of the second tube 22 component, or reduce the fluorescent reagent and the diluent of the reaction cell 3 and the second tube 22 component.
  • the contact of the reagents reduces the consumption of the first reagent of the first pipeline 12 assembly.

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Abstract

一种样本检测装置(100)和样本检测装置(100)的运行方法，样本检测装置(100)包括第一试剂组件(1)，第一试剂组件(1)包括用于容置第一试剂的第一试剂容器(11)和第一管路(12)；第二试剂组件(2)，第二试剂组件(2)包括用于容置第二试剂的第二试剂容器(21)和第二管路(22)，第二试剂与第一试剂相异；用于对生物样本进行处理以形成待反应样本的反应池(3)，反应池(3)联通第一管路(12)和第二管路(22)；以及隔离件(4)，隔离件(4)至少设置于第一管路(12)上，隔离件(4)用于减少第一试剂与反应池(3)内的试剂和/或第二试剂的接触。样本检测装置(100)通过将隔离件(4)至少设置于与第一试剂容器(11)相连的第一管路(12)上，能够减少第一试剂与反应池(3)内的试剂和/或第二试剂的接触，减少第一试剂被反应池(3)内的试剂和/或第二试剂污染的风险，减少第一试剂的消耗量。

Description

样本检测装置和样本检测装置的运行方法 技术领域
本发明涉及医疗器械技术领域，尤其涉及一种样本检测装置和样本检测装置的运行方法。
背景技术
荧光试剂是中高端血球仪产品中使用的试剂，荧光染料插入DNA沟回中改变了染料分子的结构自由度及电子状态从而改变了分子的荧光特性使荧光增强，通过激光照射细胞，不同细胞结构发出不同的光，通过接收器接收信号，经过处理后得出不同的细胞分类。
目前很多场景都需要消耗荧光试剂，导致荧光试剂消耗过快，其中消耗的荧光试剂有将近一半为非测量的场景所消耗。
发明内容
本申请提供一种样本检测装置和样本检测装置的运行方法。
一方面，本申请实施例提供了一种样本检测装置，包括：
第一试剂组件，所述第一试剂组件包括用于容置第一试剂的第一试剂容器和第一管路；
第二试剂组件，所述第二试剂组件包括用于容置第二试剂的第二试剂容器和第二管路，所述第二试剂与所述第一试剂相异；
用于对生物样本进行处理以形成待测样本的反应池，所述反应池联通第一管路和第二管路；以及
隔离件，所述隔离件至少设置于所述第一管路上，所述隔离件用于减少所述第一试剂与所述反应池内的试剂和/或所述第二试剂的接触
另一方面，本申请实施例还提供了一种样本检测装置的运行方法，应用于样本检测装置，所述样本检测装置包括第一管路、第二管路和反应池，所述第一管路和所述第二管路联通至所述反应池，所述方法包括：
对所述反应池清洗并排空；
使所述第一管路和/或所述第二管路回吸空气以形成第一气柱，并使得所述第一气柱至少部分位于所述第一管路；
往所述反应池内添加底液。
本申请实施例提供的样本检测装置通过将隔离件至少设置于与第一试剂容器相连的第一管路上，能够减少第一试剂、与反应池内的试剂和/或第二试剂的接触，从而减少第一试剂被反应池内的试剂和/或第二试剂污染的风险，减少第一试剂的消耗量。
附图说明
图1是本申请实施例提供的一种样本检测装置的示意图；
图2是图1所示的样本检测装置的样本检测方法的流程示意图；
图3是图1所示的样本检测装置中的一实施例中的反应组件的示意图；
图4图3所示的反应组件中的隔离件的示意图；
图5是3所示的反应组件中的隔离件的另一角度的示意图；
图6是图1所示的样本检测装置中的另一实施例中的反应组件的示意图；
图7是图6所示的反应组件的一种样本检测方法的流程示意图；
图8是图6所示的样本检测装置的另一种样本检测方法的流程示意图；
图9是图6所示的样本检测装置中的另一施例中的反应组件的示意图；
图10是图9所示的反应组件的样本检测方法的流程示意图；
图11是图6所示的样本检测装置中的另一施例中的反应组件的示意图；
图12是图1所示的样本检测装置中的另一实施例中的反应组件的示意图；
图13是图12所示的反应组件的一种样本检测方法的流程示意图；
图14是图12所示的样本检测装置中的另一实施例中的反应组件的示意图；
图15是图12所示的样本检测装置中的另一实施例中的反应组件的示意图；
图16是图12所示的样本检测装置中的另一实施例中的反应组件的示意图。
具体实施方式
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有作出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
请参照图1，为本申请实施例提供一种样本检测装置100，该样本检测装置100用于对样本进行检测。该样本检测装置100内的反应池3用于对样本处理形成待测样本，该待测样本能够运输到检测组件5中进行测量。需要说明的是，样本的具体种类不受限制。在一些实施例中，样本包括固定样本或者液体样本。进一步的液体样本包括但不限于血液样本。可以理解的，反应池3对样本处理形成待测样本的方式包括但不限于以下方式：样本与各种所需的试剂发生孵育反应形成待测样本。
为了方便对对样本各个阶段名称的理解，此处对样本各个阶段的名称进行详述；样本未在反应池3中进行孵育反应前定义为待反应样本，待反应样本在反应池3中与各种试剂完成孵育反应后形成的反应物定义为待测样本。
可以理解的，反应池3为了防止结晶，反应池3内容置有稀释液底液，待反应样本在反应池3中进行反应前，需要将反应池3内的稀释液底液排空，防止稀释液底液对待反应样本或者与待反应样本发生反应的试剂造成稀释，而现有技术中反应池3内的稀释液底液一般会与要与待反应样本发生孵育反应的试剂长时间接触，从而使得该试剂被稀释，换言之，该试剂会被稀释液底液污染，而被污染的试剂则需要排掉以避免待测样本的测试结果出现异常，使得试剂的消耗量较大。
针对试剂容易受到污染而导致消耗量较大的问题，本申请实施例提供了一种样本检测装置100，该样本检测装置100包括第一试剂组件1、第二试剂组件2、反应池3和隔离件4，第一试剂组件1包括用于容置第一试剂的第一试剂容器11和第一管路12；第二试剂组件2包括用于容置第二试剂的第二试剂容器21和第二管路22，第二试剂与第一试剂相异；反应池3用于对生物样本进行处理以形成待测样本，反应池3联通第一管路12和第二管路22；隔离件4至少设置于第一管路12上，隔离件4用于减少第一试剂与反应池3内的试剂和/或第二试剂的接触。
其中，第一试剂组件1中的第一试剂和第二试剂组件2中的第二试剂能够在反应池3中与待反应样本孵育反应形成待测样本。进一步的，第一试剂包括但 不限于荧光液。第二试剂包括但不限于溶血液或其他试剂液。其中，反应池3联通第一管路12和第二管路22可以理解为第一管路12内的第一试剂能够流入反应池3，第二管路22内的第二试剂能够流入反应池3。其中，隔离件4用于减少第一试剂与反应池3内的试剂和/或第二试剂的接触可以理解为隔离件4可以用于减少第一试剂与反应池3内的试剂的接触，隔离件4可以用于减少第一试剂和第二试剂的接触，隔离件4还可以用于减少第一试剂和、反应池3内的试剂和第二试剂的接触。
通过将隔离件4至少设置于第一管路12上，减少了第一管路12组件的第一试剂如荧光试剂与反应池3的稀释液的接触、或者减少了第一管路12组件的第一试剂如荧光试剂与第二管路22组件的第二试剂的接触、或者减少了荧光试剂与反应池3的稀释液和第二管路22组件的试剂的接触，从而减少了第一管路12组件的第一试剂的消耗量。
请参照图1和图3，为本申请实施例提供的样本检测装置100，该样本检测装置100中的第一管路12和第二管路22通过隔离件4联通反应池3。具体的，第一管路12具有第一接入点S1，第二管路22具有第二接入点S2，样本检测装置100还包括连接反应池3的第三管路31，第三管路31具有第三接入点S3，第一接入点S1和第二接入点S2经隔离件4联通第三接入点S3。换言之，隔离件4同时设于第一管路12和第二管路22上，并且第一管路12和第二管路22经隔离件4联通反应池3。换言之，第一管路12内的第一试剂和第二管路22内的第二试剂通过隔离件4与反应池3内的稀释底液减少接触，从而减少了第一管路12组件的第一试剂的消耗量。其中，第一试剂组件1、第二试剂组件2、反应池3和隔离件4主要用于进行孵育反应，故为了便于描述，将上述部件统称为反应组件10。
一些实施例中，如图1和图3所示，第一试剂组件1内的第一试剂及第二试剂组件2内的第一试剂经隔离件4与第三管路31内的试剂完全隔离。
一实施例中，如图1所示，隔离件4包括第一阀体和位于第一阀体内的第一阀芯，第一阀体具有两个第一接入口和一个第一接出口，第一接入点S1和第二接入点S2分别接入隔离件4的一个第一接入口，第三接入点S3接入隔离件4的第一接出口；第一阀芯相对第一阀体活动，以使第一接入点S1或第二接入点S2与第三接入点S3联通。其中，可以通过控制第一阀体和第一阀芯的通电情况来使得第三接入点S3与第一接入点S1联通、或第三接入点S3与第二接入点S2联通。可选的，第一阀体和第一阀芯处于断电状态时，第三接入点S3联通第二接入点S2，换言之，在第一阀体和第一阀芯处于断电状态时，第二管路22内的第二试剂能够流入反应池3，而第一管路12内的第一试剂由于被隔离件4隔离而无 法流入反应池3，换言之，反应池3内的试剂亦无法扩散至第一管路12内而污染第一管路12内的第一试剂，从而将第一管路12内的第一试剂与反应池3完全隔离；第一阀体和第一阀芯处于通电状态时，第一管路12内的第二试剂能够流入反应池3，而第二管路22内的第二试剂由于被隔离件4隔离而无法流入反应池3。
如图2所示，该图2为图1所示的样本检测装置100的运行方法流程图，该样本检测装置100的运行方法包括：101：排空反应池3内的稀释液底液。其中，排空稀释液底液以防止稀释液底液对后续的孵育反应产生影响。
103：将第二试剂容器21内的第二试剂第一次依次经第二管路22、第二接入点S2和第三接入点S3形成的第二通道、第三管路31流入反应池3内并排掉该第二试剂。
该步骤为防止反应池3内仍然残留的稀释液底液或污染物对后续的孵育反应产生影响。其中，该第一阀芯处于断电状态，换言之，第二接入点S2与第三接入点S3处于导通状态形成第二通道。
105：将第二试剂容器21内的第二试剂第二次依次经第二管路22、第二接入点S2和第三接入点S3形成的第二通道、第三管路31流入反应池3内。
具体的，该第二试剂用于与后续的待反应样本、第一试剂进行孵育反应形成待测样本。
107：使隔离件4处于通电状态，改变第一阀芯相对第一阀体的位置而使得第一接入点S1与第三接入点S3导通而形成第一通道；并将第一试剂容器11内的第一试剂经第一管路12、第一通道、第三管路31流入反应池3内。
具体的，该第一试剂用于与容置于反应池3内的第二试剂、和后续的待反应样本发生孵育反应形成待测样本。
109：使隔离件4处于断电状态并将第二试剂容器21内的第二试剂第三次经第二管路22、第二通道、第三管路31流入反应池3内，同时往反应池3内添加待反应样本。
具体的，该待反应样本与、第一试剂和第二试剂进行孵育反应形成待测样本。
111：将待测样本流入检测组件5中进行测量；
113：在反应池3内加入清洗液进行清洗，反应池3清洗干净后加入底液。
可以理解的，对于精度要求不高的测试条件下，该样本检测装置100的运行方法中的第二试剂亦可以只添加一次，换言之步骤103、步骤105可以省略。可以理解的，图2所示的样本检测装置100的运行方法中的第一试剂、第二试剂和待反应样本流入反应池的先后顺序乃是可选的一种实施例，可选的，第一试 剂、第二试剂和待反应样本添加的先后顺序可以改变。例如，第一试剂、第二试剂和待反应样本可以同时添加，或依次添加等。
可以理解的，该待反应样本为血液样本。进一步的，底液可以为稀释液。
本申请实施例提供的样本检测装置100通过将隔离件4设置成包括第一阀体和第一阀芯的结构，使得只有在需要在往反应池3内添加第一试剂时才会导通第一管路12和反应池3，从而使得反应池3内的稀释液和第二管路22内的第二试剂被隔离件4完全隔离而不会扩散到第一管路12中，从而使得第一试剂容器11内的第一试剂不会被反应池3内的稀释液和第二管路22内的第二试剂污染，从而减少了第一试剂的消耗量。
另一实施例中，如图3所示，图3为图1提供的样本检测组件5的反应组件10的示意图，隔离件4还可以为以下结构实现第一试剂组件1内的第一试剂及第二试剂组件2内的第二试剂经隔离件4与第三管路31内的试剂完全隔离。具体的，请一并参照图4和图5，图4和图5为图3所示的反应组件10中的隔离件4的示意图。隔离件4包括第二阀体43和位于第二阀体43内的第二阀芯44，第二阀体43具有两个第二接入口43a和一个第二接出口43b，第一接入点S1和第二接入点S2分别接入隔离件4的一个第二接入口43a，第三接入点S3接入隔离件4的第二接出口43b；当第二阀芯44位于第二阀体43的第一位置时，第一接入点S1与第三接入点S3的连接断开，第二接入点S2与第三接入点S3联通；当第二阀芯44位于第二阀体43的第二位置时，第一接入点S1与第三接入点S3联通，第二接入点S2与第三接入点S3联通。
可选的，两个第二接入口43a和一个第二接出口43b位于第二阀体43的同一侧，便于管路接入第二阀体43上。进一步的，用以接入第一管路12的第二接入口43a位于用于接入第二管路22的第二接入口43a和用于接入第三管路31的第二接出口43b之间。在隔离件4处于断电状态时，第二阀体43堵住用以接入第一管路12的第二接入口43a，对应的，用于接入第二管路22的第二接入口43a与第二接口相连通，以使第二管路22内的第二试剂能够流入反应池3；在隔离件4处于通电状态时，第二阀体43朝着远离用以接入第一管路12的第二接入口43a的方向运动，以使用以接入第一管路12的第二接入口43a与第二接出口43b联通，从而使得第一管路12内的第一试剂能够流入反应池3。
该样本检测装置100的运行方法流程请参照步骤101-113，在此不再赘述。
一些实施例中，如图6、图9和图11所示，隔离件4还可以将第一试剂与其它试剂不完全隔离而减少第一试剂与其它试剂的接触而减少第一管路12内的第一试剂的消耗量。具体的，如图6所示，隔离件4具有接头45，接头45具有一 个内腔和联通内腔的三个接口，第一接入点S1、第二接入点S2和第三接入点S3分别接入接头45的一个接口。可选的，接头45进入第一接入点S1的接口的内径为0.1mm～0.35mm。具体的，接头45可为三通接头45，通过改变接头45与第一接入点S1连接的接口的内径，从而使得第二管路22内的第二试剂或反应池3内的试剂难以经扩散至第一管路12内，从而减少了第一管路12内的第一试剂与其他试剂的接触，以减少第一管路12内的第一试剂的消耗量。
如图7所示，图7为图6所示的样本检测装置100的运行方法流程图，该样本检测装置100的运行方法包括：：
201：排空反应池3内的稀释液底液。其中，排空稀释液底液以防止稀释液底液对后续的孵育反应产生影响。
203：将第二试剂容器21内的第二试剂第一次依次经第二管路22、接头45、第三管路31流入反应池3内并排掉该第二试剂，以防止反应池3内仍然残留的稀释液底液对后续的孵育反应产生影响。
205：将第二试剂容器21内的第二试剂第二次依次经第二管路22、接头45、第三管路31流入反应池3内。该第二试剂用于与后续的待反应样本、第一试剂进行孵育反应形成待测样本。
207：将第一试剂容器11内的第一试剂经第一管路12、接头45、第三管路31流入反应池3内。该第一试剂用于与容置于反应池3内的第二试剂、和后续的待反应样本发生孵育反应形成待测样本。
209：将第二试剂容器21内的第二试剂第三次经第二管路22、接头45、第三管路31流入反应池3内，同时往反应池3内添加待反应样本。该待反应样本与、第一试剂和第二试剂进行孵育反应形成待测样本。
211：将待测样本流入检测组件5中进行测量；
213：在反应池3内加入清洗液进行清洗，反应池3清洗干净后加入底液。
可以理解的，对于精度要求不高的测试条件下，该样本检测装置100的运行方法中的第二试剂亦可以只添加一次，换言之步骤203、步骤205可以省略。可以理解的，图7所示的样本检测装置100的运行方法中的第一试剂、第二试剂和待反应样本添加的先后顺序乃是可选的一种实施例，第一试剂、第二试剂和待反应样本添加的先后顺序可以改变。例如，第一试剂、第二试剂和待反应样本可以同时添加，或依次添加，等。
本申请实施例提供的样本检测装置100通过将隔离件4设置成接头45，该接头45分别与第一管路12、第二管路22和第三管路31联通，且该接头45与第一管路12相连接的接口的内径较小，以能够较佳的减少第二管路22或第三管路31 内的试剂扩散至第一管路12内，从而能够减少第一管路12的第一试剂的消耗量。
一实施例中，请参阅图6，该隔离件4还包括第一气柱，第一气柱位于第一管路12，且靠近第一试剂容器11。第一气柱由空气形成，该第一气柱位于第一管路12内时，能够有效避免其他试剂扩散至该第一管路12内，从而防止该第一管路12内的试剂被污染，从而减少该第一管路12内的试剂的消耗量。
请参照图8，图8为图6所示的样本检测装置100的运行方法流程图，该样本检测装置100的运行方法运行在反应池3形成一次待侧样本后对反应池3清洗并排空的步骤后，换言之，该方法包括：
301：对反应池清洗并排空；
303：使第一管路回吸空气以形成第一气柱，并使得第一气柱至少部分位于第一管路；
305：往反应池内添加底液。
可选的，当样本检测装置100需要在反应池3内形成下一次待测样本即进行样本测试时，该样本检测装置100的运行方法流程请参照步骤201至213，在此不再赘述。其中，在流入过程中消除第一气柱。换言之，形成有第一气柱的管路即第一管路12在第一试剂经过时，第一气柱会被打破。当然，在其它实施例中，第一气柱还会进入第二管路22，换言之，第一气柱还可以位于第二管路22中。若第一气柱位于第二管路22中，则在步骤301可以对应为使第一管路12和第二管路22回吸空气形成第一气柱，则在第二管路22中有首次要经过第一气柱时，第一气柱会被打破。当然，在其它实施例中，第一气柱还可以仅位于第二管路22中。
本申请实施例提供的样本检测装置100将隔离件4设置成接头45和位于第一管路12和/或第二管路22内的第一气柱，该第一气柱位于管路内时，能够有效避免其他试剂扩散至该管路内，从而防止该管路内的试剂被污染，从而减少该管路内的试剂的消耗量。
另一实施例中，如图9所示，隔离件4还具有两通阀46，两通阀46设于第一管路12上，且位于第一试剂容器11与第一接入点S1之间。可选的，两通阀46的内部容积较小，且两通阀46靠近接头45。两通阀46能够有效的防止其它试剂经接头45流入第一试剂容器11内，从而减少第一试剂的消耗量。
请参照图10，图10为图9所示的样本检测装置100的运行方法流程图，该样本检测装置100的运行方法包括：401：排空反应池3内的稀释液底液。其中，排空稀释液底液以防止稀释液底液对后续的孵育反应产生影响。
403：将第二试剂容器21内的第二试剂第一次依次经第二管路22、接头45、第三管路31流入反应池3内并排掉该第二试剂，以防止反应池3内仍然残留的稀释液底液对后续的孵育反应产生影响。
405：将第二试剂容器21内的第二试剂第二次依次经第二管路22、接头45、第三管路31流入反应池3内。该第二试剂用于与后续的待反应样本、第一试剂进行孵育反应形成待测样本。
407：开启两通阀46，将第一试剂容器11内的第一试剂经第一管路12、两通阀46、接头45、第三管路31流入反应池3内后关闭两通阀46。该第一试剂用于与容置于反应池3内的第二试剂、和后续的待反应样本发生孵育反应形成待测样本。
409：将第二试剂容器21内的第二试剂第三次经第二管路22、接头45、第三管路31流入反应池3内，同时往反应池3内添加待反应样本。该待反应样本与、第一试剂和第二试剂进行孵育反应形成待测样本。
411：将待测样本流入检测组件5中进行测量；
413：在反应池3内加入清洗液进行清洗，反应池3清洗干净后加入底液。
再一实施例中，如图11所示，隔离件4还具有单向阀47，单向阀47设于第一管路12上，且位于第一试剂容器11与第一接入点S1之间。
可选的，接头45接入第一接入点S1的接口接入单向阀47的出口。换言之，接头45和单向阀47做成一体。换言之，接头45与单向阀47之间无需胶管连接。该结构的隔离件4使得第一管路12内的第一试剂能够从隔离件4流入反应池3，而对应的由于单向阀47的存在使得第二管路22的第二试剂和反应池3内的试剂无法经单向阀47流经第一试剂容器11，避免第一试剂被其他试剂污染，从而减少了第一试剂的消耗量。
该样本检测装置100的反应流程大致与步骤401至413相同，其不同之处在于：将第一试剂容器11内的第一试剂经第一管路12、单向阀47、接头45、第三管路31流入反应池3内。该第一试剂用于与容置于反应池3内的第二试剂、和后续的待反应样本发生孵育反应形成待测样本。
如图12至图17所示，为本申请实施例提供的反应组件10，该反应组件10通过将第一管路12和第二管路22分别接入反应池3来完全隔绝第一管路12的第一试剂和第二管路22的第二试剂之间的相互污染，且隔离件4仅设置在第一管路12上来减少第一管路12的第一试剂与反应池3的试剂的接触，从而减少第一管路12的第一试剂的消耗量。具体的，第一管路12和第二管路22分别连接反应池3，隔离件4设置于第一管路12上。
一实施例中，如图12所示，第一管路12连接反应池3的一段形成有隔离件4，隔离件4内径为第一管路12的最小内径，且内径范围为0.1mm～0.35mm。具体的，该隔离件4可以为第一管路12的一部分，该隔离件4还可以为套设于第一管路12的端部的钢管，且该钢管的内径为0.1mm～0.35mm。通过使得第一管路12连接反应池3的一段的内径为第一管路12的最小内径，使得反应池3内的试剂扩散至第一管路12内的扩散速度，以减少第一管路12内的第一试剂被反应池3内的试剂污染的量，从而减少第一管路12的第一试剂的消耗量。
请参照图13，图13为图12所示的该样本检测装置100的运行方法流程图，该样本检测装置100的运行方法包括：
501：排空反应池3内的稀释液底液。其中，排空稀释液底液以防止稀释液底液对后续的孵育反应产生影响。
503：将第二试剂容器21内的第二试剂第一次经第二管路22流入反应池3内并排掉该第二试剂，以防止反应池3内仍然残留的稀释液底液对后续的孵育反应产生影响。
505：将第二试剂容器21内的第二试剂第二次经第二管路22流入反应池3内。该第二试剂用于与后续的待反应样本、第一试剂进行孵育反应形成待测样本。
507：将第一试剂容器11内的第一试剂经第一管路12流入反应池3内。该第一试剂用于与容置于反应池3内的第二试剂、和后续的待反应样本发生孵育反应形成待测样本。
509：将第二试剂容器21内的第二试剂第三次经第二管路22流入反应池3内，同时往反应池3内添加待反应样本。该待反应样本与、第一试剂和第二试剂进行孵育反应形成待测样本。
511：将待测样本流入检测组件5中进行测量；
513：在反应池3内加入清洗液进行清洗，反应池3清洗干净后加入底液。
另一实施例中，如图12所示，隔离件4为第二气柱，第二气柱位于第一管路12，用于阻断第一试剂和反应池3的联通。换言之，该第一管路12和第二管路12乃是分别联通反应池3，第一管路12回吸空气形成第二气柱。具体的，第二气柱由空气形成，该第二气柱位于第一管路12内时，能够有效避免其他试剂扩散至第一管路12内，从而防止第一管路12内的试剂被污染，从而减少第一管路12内的试剂的消耗量。可以理解的，此处的第二气柱可以与前述的第一气柱相同。
该样本检测装置100的运行方法运行在反应池3形成一次待测样本后对反 应池3清洗并排空的步骤后，具体的流程请参照步骤301至305，在此不再赘述。
可选的，当样本检测装置100需要在反应池3内形成下一次待测样本时，该样本检测装置100的运行方法流程请参照步骤501至513，在此不再赘述。其中，形成有第一气柱的管路即第一管路12在第一试剂经过时，第一气柱会被打破。
再一实施例中，如图14和图15所示，隔离件4为串联于第一管路12中的两通阀或单向阀。
可选的，如图14所示，隔离件4可以为串联于第一管路12中的两通阀，两通阀的内部容积较小，且两通阀靠近接头45。两通阀能够有效的防止反应池3内的试剂流入第一试剂容器11内，从而减少第一试剂的消耗量。
可选的，如图15所示，隔离件4可以为串联于第一管路12内的单向阀，该结构的隔离件4使得第一管路12内的第一试剂能够从隔离件4流入反应池3，而对应的由于单向阀的存在使得反应池3内的试剂无法经单向阀流经第一试剂容器11，避免第一试剂被其他试剂污染，从而减少了第一试剂的消耗量。
该样本检测装置100的反应流程大致与步骤501至513相同，其不同之处在于，将第一试剂容器11内的第一试剂经第一管路12、二通阀或单向阀流入反应池3内。
再再一实施例中，如图16所示，第二管路22连接反应池3，第一管路12经隔离件4联通反应池3，隔离件4为套管，且悬空设于反应池3上。具体的，第一管路12悬空设置于反应池3之上，减少第一管路12与反应池3之间相互扩散的速度，从而减少第一管路12内的第一试剂的消耗量。
该样本检测装置100的反应流程请参照步骤501至513，在此不再赘述。
进一步的，如图1所示，该样本检测装置100还包括主管路6和检测组件5，主管路6具有第四接入点S4，反应池3接入主管路6的第四接入点S4上；检测组件5包括括样本针51，样本针51具有第五接入点S5，反应池3的待测样本经主管路6的第四接入点S4流动至样本针51的第五接入点S5，以使得反应池3中的待测样本流入检测组件5中的样本中来供检测。
可选的，样本检测装置100还包括连接反应池3和主管路6之间的第四管路32、设于第四管路32上的第一切换件33，其中，第一切换件33位于第四接入点S4和主管路6之间。若第一切换件33开启即第一切换件33实现反应池3与主管路6的联通时，反应池3内反应完成的待测样本可以通过依次经第四管路32和第四接入点S4流动至样本针51的第五接入点S5，以进入检测组件5进行检测。若第一切换件33关闭即第一切换件33实现内第四管路32的切断时，则第四管路32中的待测液无法流入主管路6。
可选的，样本检测装置100还包括连接第四接入点S4的第五管路61和连接第五管路61的第一稀释液池62。第一稀释液池62中的稀释液能够经第五管路61、第四接入点S4和第第四管路32流入反应池3内。
可选的，样本检测装置100还包括连接反应池3的排液池34。该排液池34能够用于存储反应池3排出的液体。
可选的，检测组件5还包括流动室52、鞘液池53和第一废液池54。流动室52具有待测样本入口、鞘液入口以及出口，该待测样本入口连接样本针51的第五接入点S5，鞘液入口连接鞘液池53，鞘液池53用于存储鞘液，出口用以连接第一废液池54。
可选的，鞘液池53连接第一储气罐，两者连通时，第一储气罐提供的第一正压推动鞘液流入流动室52。
检测组件5进行检测时，待测样本自待测样本入口进入流动室52，鞘液池53中的鞘液可以经鞘液入口流入流动室52中，且鞘液包裹该待测样本形成待测样本流进行检测，检测后的待测样本流自出口进入第一废液池54。
进一步的，样本检测装置100还包括推样组件7，通过设置推样组件7来将待测样本准备段的待测样本推入检测组件5的样本针51中。
推样组件7分别接入主管路6的第六接入点S6，第六接入点S6位于第四接入点S4和第五接入点S5之间，第六接入点S6与第五接入点S5之间的通道为待测样本准备段；反应池3中的待测样本经主管路6的第四接入点S4流动至主管路6的第六接入点S6，并经主管路6的第六接入点S6流入待测样本准备段；推样组件7用于将待测样本准备段内的待测样本推入检测组件5的样本针51中。
可选的，推样组件7包括注射器71、第六管路72、设于第六管路72上的第二切换件73和第二稀释液池74。第二切换件73开启时，第二稀释液池74内的稀释液能够流入注射器71；注射器71中的液体自第六接入点S6推入第五接入点S5，以将待测样本准本段内的待测样本推入第五接入点S5，以供检测组件5检测。第二切换件73断开时则切断第六管路72。
可选的，样本检测装置100还包括设于主管路6上的第三切换件63，该第三切换件63位于第六接入点S6和第四接入点S4之间。第三切换件63开启时，第四接入点S4与待测样本准备段导通。第三切换件63关闭时，主管路6切断。
进一步的，样本检测装置100还包括抽样组件8，抽样组件8接入主管路6的端部，抽样组件8用于形成负压以将反应池3中的待测样本抽取到待测样本准备段内。
可选的，抽样组件8包括第二废液池81，第二废液池81内形成负压，该负 压将反应池3中的待测样本抽取到待测样本准备段内。
该样本检测装置100需要进行检测时，首先在反应池3内进行孵育反应(其中反应组件10具体反应的流程请参照前述实施例提及的反应流程，在此不再赘述)，孵育反应完后的待侧样本流入检测组件5中进行检测。其中，待测样本的流经路径为：使抽样组件8形成负压、开启第一切换件33和第三切换件63，使得待测样本从反应池3中依次流经第四管路32、第四接入点S4、主管路4直至流入待测样本准备段内，开启第二切换件73，第二稀释液池74内的稀释液流入注射器71；注射器71中的液体自第六接入点S6推入第五接入点S5，以将待测样本准本段内的待测样本推入第五接入点S5，以供检测组件5检测；检测组件5进行检测时，待测样本自待测样本入口进入流动室52，鞘液池53中的鞘液可以经鞘液入口流入流动室52中，且鞘液包裹该待测样本形成待测样本流进行检测，检测后的待测样本流自出口进入第一废液池54。
本申请实施例提供的样本检测装置100通过将隔离件4至少设置于第一管路12上，减少了第一管路12组件的第一试剂如荧光试剂与反应池3的稀释液的接触、或者减少了第一管路12组件的第一试剂如荧光试剂与第二管路22组件的第二试剂的接触、或者减少了荧光试剂与反应池3的稀释液和第二管路22组件的试剂的接触，从而减少了第一管路12组件的第一试剂的消耗量。

Claims (25)

  1. 一种样本检测装置，其特征在于，包括：
    第一试剂组件，所述第一试剂组件包括用于容置第一试剂的第一试剂容器和第一管路；
    第二试剂组件，所述第二试剂组件包括用于容置第二试剂的第二试剂容器和第二管路，所述第二试剂与所述第一试剂相异；
    用于对生物样本进行处理以形成待测样本的反应池，所述反应池联通第一管路和第二管路；以及
    隔离件，所述隔离件至少设置于所述第一管路上，所述隔离件用于减少所述第一试剂与所述反应池内的试剂和/或所述第二试剂的接触。
  2. 根据权利要求1所述的样本检测装置，其特征在于，所述第一管路具有第一接入点，所述第二管路具有第二接入点，所述样本检测装置还包括连接所述反应池的第三管路，所述第三管路具有第三接入点，所述第一接入点和所述第二接入点经所述隔离件联通所述第三接入点。
  3. 根据权利要求2所述的样本检测装置，其特征在于，所述第一试剂组件内的第一试剂及所述第二试剂组件内的第二试剂经所述隔离件与所述第三管路内的试剂完全隔离。
  4. 根据权利要求3所述的样本检测装置，其特征在于，所述隔离件包括第一阀体和位于所述第一阀体内的第一阀芯，所述第一阀体具有两个第一接入口和一个第一接出口，所述第一接入点和所述第二接入点分别接入所述隔离件的一个第一接入口，所述第三接入点接入所述隔离件的第一接出口；所述第一阀芯相对所述第一阀体活动，以使所述第一接入点或所述第二接入点与所述第三接入点联通。
  5. 根据权利要求3所述的样本检测装置，其特征在于，所述隔离件包括第二阀体和位于所述第二阀体内的第二阀芯，所述第二阀体具有两个第二接入口和一个第二接出口，所述第一接入点和所述第二接入点分别接入所述隔离件的一个第二接入口，所述第三接入点接入所述隔离件的第二接出口；当所述第二阀芯位于所述第二阀体的第一位置时，所述第一接入点与所述第三接入点的 连接断开，所述第二接入点与所述第三接入点联通；当所述第二阀芯位于所述第二阀体的第二位置时，所述第一接入点与所述第三接入点联通，所述第二接入点与所述第三接入点联通。
  6. 根据权利要求2所述的样本检测装置，其特征在于，所述隔离件具有接头，所述接头具有一个内腔和联通所述内腔的三个接口，所述第一接入点、所述第二接入点和所述第三接入点分别接入所述接头的一个接口。
  7. 根据权利要求6所述的样本检测装置，其特征在于，所述接头接入所述第一接入点的接口的内径为0.1mm～0.35mm。
  8. 根据权利要求6或7所述的样本检测装置，其特征在于，所述隔离件还包括第一气柱，所述第一气柱至少部分位于所述第一管路和/或第二管路，且靠近所述第二试剂容器。
  9. 根据权利要求1或7所述的样本检测装置，其特征在于，所述隔离件还具有两通阀，所述两通阀设于所述第一管路上，且位于所述第一试剂容器与所述第一接入点之间。
  10. 根据权利要求1或7所述的样本检测装置，其特征在于，所述隔离件还具有单向阀，所述单向阀设于所述第一管路上，且位于所述第一试剂容器与所述第一接入点之间。
  11. 根据权利要求10所述的样本检测装置，其特征在于，所述接头接入所述第一接入点的接口接入所述单向阀的出口。
  12. 根据权利要求1所述的样本检测装置，其特征在于，所述第一管路和所述第二管路分别连接所述反应池，所述隔离件设置于所述第一管路上。
  13. 根据权利要求12所述的样本检测装置，其特征在于，所述第一管路连接所述反应池的一段形成有所述隔离件，所述隔离件内径为所述第一管路的最小内径，且内径范围为0.1mm～0.35mm。
  14. 根据权利要求12所述的样本检测装置，其特征在于，所述隔离件为串联于所述第一管路中的两通阀或单向阀。
  15. 根据权利要求12所述的样本检测装置，其特征在于，所述隔离件为第二气柱，所述第二气柱位于所述第一管路，用于阻断第一试剂和所述反应池的联通。
  16. 根据权利要求1所述的样本检测装置，其特征在于，所述第二管路连接所述反应池，所述第一管路经所述隔离件联通所述反应池，所述隔离件为套管，且悬空设于所述反应池上。
  17. 根据权利要求1至16任意一项所述的样本检测装置，其特征在于，所述第一试剂组件用于容置荧光试剂。
  18. 根据权利要求17所述的样本检测装置，其特征在于，所述第二试剂组件用于容置溶血剂、稀释液中的至少一种。
  19. 根据权利要求1至18任意一项所述的样本检测装置，其特征在于，所述样本检测装置还包括：
    主管路，所述主管路具有第四接入点，所述反应池接入所述主管路的第四接入点上；
    检测组件，包括样本针，所述样本针具有第五接入点，所述第一反应池的待测样本经所述主管路的第四接入点流动至所述样本针的第五接入点，以使得所述第一反应池中的待测样本流入所述检测组件中的样本中来供检测。
  20. 根据权利要求19所述的样本检测装置，其特征在于，所述样本检测装置还包括推样组件，所述推样组件分别接入所述主管路的第六接入点，所述第六接入点位于所述第四接入点和所述第五接入点之间，所述第六接入点与所述第五接入点之间的通道为待测样本准备段；
    所述反应池中的待测样本经所述主管路的第四接入点流动至所述主管路的第六接入点，并经所述主管路的第六接入点流入所述待测样本准备段；
    所述推样组件用于将所述待测样本准备段内的待测样本推入所述检测组件的样本针中。
  21. 根据权利要求20所述的样本检测装置，其特征在于，所述样本检测装置还包括抽样组件，所述抽样组件接入所述主管路的端部，所述抽样组件用于形成负压以将所述反应池中的待测样本抽取到所述待测样本准备段内。
  22. 一种样本检测装置的运行方法，应用于样本检测装置，所述样本检测装置包括第一管路、第二管路和反应池，所述第一管路和所述第二管路联通至所述反应池，其特征在于，所述方法包括：
    对所述反应池清洗并排空；
    使所述第一管路和/或所述第二管路回吸空气以形成第一气柱，并使得所述第一气柱至少部分位于所述第一管路；
    往所述反应池内添加底液。
  23. 根据权利要求22所述的方法，其特征在于，所述第一管路和所述第二管路分别联通所述反应池，其中，“使所述第一管路和/或所述第二管路回吸空气形成第一气柱”中，包括：
    使所述第一管路回吸空气形成第一气柱。
  24. 根据权利要求22所述的方法，其特征在于，所述样本装置还包括第三管路，所述第一管路和所述第二管路共同经所述第三管路联通所述反应池，其中，“使所述第一管路和/或所述第二管路回吸空气形成第一气柱”中，包括：
    使所述第一管路或第二管路回吸空气形成第一气柱。
  25. 根据权利要求22所述的方法，其特征在于，进行样本测试时，所述方法还包括：
    将所述反应池中的底液排空；
    使所述第一管路和/或所述第二管路中的试剂流入所述反应池，其中，在流入过程中消除所述第一气柱。
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