WO2020227852A1

WO2020227852A1 - System for large-scale production of rh2 by means of enzymatic method

Info

Publication number: WO2020227852A1
Application number: PCT/CN2019/086413
Authority: WO
Inventors: 周志刚; 蒋小龙; 张琦
Original assignee: 邦泰生物工程(深圳)有限公司; 邦泰合盛生物科技（深圳）有限公司
Priority date: 2019-05-10
Filing date: 2019-05-10
Publication date: 2020-11-19
Also published as: CN212077054U

Abstract

Disclosed is a system for large-scale production of Rh2 by means of an enzymatic method. The production system comprises a reaction kettle, a cooling water circulation device, a hot steam circulation device, an acid storage tank, an alkali storage tank, a measurement device for measuring the degree of reaction in the reaction kettle, a primary solid-liquid separation device for solid-liquid separation treatment of the material from the reaction kettle, a washing tank for cleaning the material subjected to primary solid-liquid separation, a secondary solid-liquid separation device for solid-liquid separation treatment of the material from the water washing tank, and a drying device for drying the material subjected to secondary solid-liquid separation. The production system has a simple structure, complete functions and a compact layout, saves the space and costs, can implement automatic control of a temperature and a pH value, and saves labor.

Description

Rh2 enzymatic large-scale production system

Technical field

The invention relates to the technical field of biocatalysis technology, in particular to an industrial production system that utilizes bioenzyme catalytic technology to produce Rh2 on a large scale.

Background technique

Rh2 is one of the main pharmacologically active ingredients in ginseng saponins-one of ginsenosides. It is a monomer component with significant anti-cancer activity in ginsenosides. It almost represents all the positive effects of ginseng and is regarded as the best Potential natural anti-cancer substance.

Because the content of Rh2 in Panax genus medicinal materials is extremely scarce, and extraction is very difficult, companies represented by Bangtai Biotechnology have developed a method for producing Rh2 using biological enzyme catalysis technology, which has the advantages of simple operation, short time, energy saving and environmental protection. Although there have been many reports on the production of Rh2 by biological enzyme catalytic technology, there are no reports on the industrial production system of Rh2 by enzymatic method. It is well-known in the industry that when a new process is applied to large-scale industrial production from small-scale and pilot-scale, there is actually a considerable degree of difficulty in technical transformation. These technical problems often hinder the Rh2 enzymatic preparation process. Used in large-scale industrial production.

technical problem

In view of the above-mentioned shortcomings in the prior art, the purpose of the present invention is to provide a system suitable for large-scale production of Rh2 enzymatically, so that the currently developed Rh2 enzymatic production process can be more conveniently, quickly and directly applied to scale. In the chemical industry production, thus filling the blank of the Rh2 enzymatic industrial production system.

Technical solutions

In order to achieve the above objective, the present invention provides a Rh2 enzymatic large-scale production system, which includes a reactor, a cooling water circulation device, a hot steam circulation device, an acid storage tank, an alkali storage tank, and a reactor for detecting The detection device for the degree of reaction, a solid-liquid separation device for solid-liquid separation treatment of materials from the reactor, a washing tank for cleaning the materials after a solid-liquid separation, and a A secondary solid-liquid separation device for solid-liquid separation processing of the material, and a drying device for drying the material after the secondary solid-liquid separation; wherein:

The reactor is equipped with a feeding port, a discharging port, a thermometer for measuring the temperature in the kettle, a pH meter for measuring the pH value in the kettle, and a stirring device for stirring the materials in the kettle. There is a jacket layer for the circulation of cooling water and hot steam;

The cooling water circulation device communicates with the jacket layer of the reactor through a pipeline, and is used to provide cooling water for the jacket layer of the reactor to lower the temperature in the reactor;

The hot steam circulation device communicates with the jacket layer of the reactor through a pipeline, and is used to provide the jacket layer of the reactor with hot steam for raising the temperature in the reactor;

The acid storage tank is connected with the inside of the reaction kettle through a pipeline;

The alkali storage tank is connected with the inside of the reaction kettle through a pipeline;

The primary solid-liquid separation device is located below the discharge port of the reactor;

The washing tank includes a tank body and a stirring device for stirring the materials in the tank body;

The secondary solid-liquid separation device is arranged below the discharge port of the washing tank.

The inner walls of the reaction kettle, water washing tank, acid storage tank, and alkali storage tank in the above production system are all equipped with acid and alkali resistant corrosion layers, or the corresponding tank body and tank body are made of acid and alkali resistant materials.

The detection device in the above-mentioned production system may be any currently known device capable of detecting the progress of the reaction in the reactor, such as a liquid chromatograph.

The solid-liquid separation device in the above-mentioned production system can be any device that can separate solids and liquids in materials without destroying the components in the materials, such as centrifuges, plate and frame filter presses, and membranes. Concentration equipment, etc.

The drying device in the above-mentioned production system can be any device that can remove moisture from Rh2 products without reducing the content of Rh2 and not destroying the structure of Rh2, such as vacuum dryers, freeze dryers, etc.

Preferably, in the above-mentioned production system provided by the present invention, the acid storage tank and the alkali storage tank are arranged above the reaction vessel, so that the acid liquid and the alkaline liquid can flow into the reaction vessel naturally by gravity, thereby reducing the use of pumps.

Preferably, the above-mentioned production system provided by the present invention further includes a condensing device for cooling the heated and volatilized solvent in the reactor of the reactor, the condensing device is arranged above the reactor and is connected to the reactor through a pipe to form a supply The cooled solvent flows back to the channel in the kettle.

Preferably, the above-mentioned production system provided by the present invention further includes a transfer device for transferring materials, by which the materials can be conveniently put into the reactor, and the materials after a solid-liquid separation can be transferred to the washing tank, or The material after the secondary solid-liquid separation is transferred to the drying device. The transfer device may be a plurality of fixed devices respectively arranged beside each device that needs to transfer materials, or one or more non-fixed devices that can move freely among multiple devices that need to transfer materials. From the perspective of resource and space saving, the transfer device is preferably a non-fixed device, and preferably, the transfer device is a traveling vehicle.

In order to achieve system automation, reduce manual operations, and reduce labor costs, preferably, the above-mentioned production system provided by the present invention further includes an automatic control system, and the automatic control system includes a temperature automatic control system and a pH automatic control system, wherein:

The temperature automatic control system includes a temperature reading device, a temperature control unit, a cooling water solenoid valve and a hot steam solenoid valve; among them, one end of the temperature reading device is electrically connected to the thermometer, the other end is electrically connected to the temperature control unit, and the cooling water solenoid valve is provided On the cooling water circulation device, it is used to control the opening and closing of the cooling water circulation device. The hot steam solenoid valve is installed on the hot steam circulation device to control the opening and closing of the hot steam circulation device. The temperature control unit controls the cooling water solenoid valve separately And hot steam solenoid valve opening and closing;

The pH automatic control system includes a pH reading device, a pH control unit, an acid solenoid valve and a lye solenoid valve; among them, one end of the pH reading device is electrically connected to the pH meter, the other end is electrically connected to the pH control unit, and the acid solenoid valve Set on the pipe connecting the acid storage tank and the reaction kettle and close to one end of the reaction kettle, used to control the delivery and stop of the acid liquid into the reaction kettle, and the lye solenoid valve is set on the pipe connecting the alkali storage tank and the reaction kettle The upper part and one end close to the reaction kettle are used to control the delivery and stop of the lye into the reaction kettle. The pH control unit controls the opening and closing of the acid solenoid valve and the lye solenoid valve respectively.

In order to better prevent environmental pollution and achieve sustainable development, preferably, the above-mentioned production system provided by the present invention further includes a sewage treatment system for purifying liquid after solid-liquid separation treatment.

Beneficial effect

The Rh2 enzymatic large-scale production system provided by the invention has simple structure, perfect function, compact arrangement, space and cost saving, automatic control of temperature and pH value, and labor saving. In addition, a separate water washing kettle and corresponding solid-liquid separation device can be set up to realize continuous and uninterrupted reaction of two batches of materials.

Description of the drawings

Figure 1 is a schematic diagram of the structure of the Rh2 enzymatic large-scale production system provided by an embodiment of the present invention;

In the picture:

1. Reactor, 11. Feeding port, 12. Discharge port, 13. Sampling port, 14. Jacket layer, 141. Hot steam inlet, 142. Cooling water inlet, 143. Hot steam outlet, 144. Cooling water outlet , 15. Stirrer, 16. Thermometer, 17. pH meter, 2. Washing tank, 21. Outlet, 22. Stirrer, 3. Acid storage tank, 31. Acid solenoid valve, 4. Alkali storage tank, 41. Lye solenoid valve, 5. Condenser, 51. Condensate inlet, 52. Condensate outlet, 6. Hot steam circulation device, 61. Hot steam solenoid valve, 7. Cooling water circulation device, 71. Cooling water solenoid valve , 8. Centrifuge, 9. Sewage treatment device.

Embodiments of the invention

The present invention will be further described in detail below with reference to the drawings and specific embodiments. The following embodiments are an explanation of the present invention, and the present invention is not limited to the following embodiments.

As shown in Figure 1, this embodiment provides a large-scale Rh2 enzymatic production system. The production system includes a reactor 1, a washing tank 2, an acid storage tank 3, an alkali storage tank 4, a condenser 5, and a hot steam cycle. Device 6, cooling water circulation device 7, centrifuge 8, sewage treatment device 9, driving (not shown), liquid chromatograph (not shown) and vacuum dryer (not shown), reactor 1, washing tank 2 , The inner walls of the acid storage tank 3 and the alkali storage tank 4 are provided with an acid and alkali resistant corrosion layer. among them:

The acid storage tank 3 and the alkali storage tank 4 are arranged above the reactor 1 and are respectively communicated with the inside of the reactor 1 through pipes, and an acid solenoid valve 31 is respectively provided at one end of the respective pipes close to the reactor 1 And lye solenoid valve 41;

The top of the reactor 1 is provided with a feeding port 11, the bottom is provided with a discharge port 12 and a sampling port 13, and the outer wall is provided with a jacket layer 14. The upper part of the jacket layer 14 is provided with a hot steam inlet 141 and a cooling water outlet 144, respectively. The lower part of the jacket layer 14 is respectively provided with a cooling water inlet 142 and a hot steam outlet 143, and the hot steam inlet 141 and the hot steam outlet 143 are respectively arranged on both sides of the reactor, and the cooling water inlet 142 and the cooling water outlet 144 are respectively arranged On both sides of the reactor; the reactor 1 is also provided with a stirrer 15, a thermometer 16 and a pH meter 17, which extend into the reactor, and a condenser 5 that communicates with the interior of the reactor 1 through a pipe;

The hot steam circulation device 6 is respectively connected with the hot steam inlet 141 and the hot steam outlet 143 through pipes to form a circulation of hot steam in the jacket layer 14. The hot steam circulation device 6 is opened and closed by the hot steam solenoid valve 61;

The cooling water circulation device 7 is communicated with the cooling water inlet 142 and the cooling water outlet 144, and the condensate inlet 51 and the condensate outlet 52 of the condenser 5 through pipelines, forming a circulation of cooling water in the jacket layer 14 and in the condensing The cooling water circulation device 7 transmits and stops the cooling water circulation in the jacket layer 14 through the cooling water solenoid valve 71 independently provided on the cooling water circulation pipeline;

The centrifuge 8 in this embodiment is composed of a primary centrifuge located below the reactor 1 and a secondary centrifuge located below the washing tank 2. The feed inlet of the primary centrifuge is connected to the outlet of the reactor 1 through a pipe. The port 12 is connected, the feed port of the secondary centrifuge is connected to the discharge port 21 of the washing tank 2 through a pipe, and the liquid outlet of the centrifuge 8 is connected to the sewage treatment device 9 through a pipe;

In addition, the Rh2 enzymatic large-scale production system provided in this embodiment also includes a temperature automatic control system and a pH automatic control system. The temperature automatic control system consists of a temperature reading device (not shown), a temperature control unit (not shown), and a thermal The steam solenoid valve 61 and the cooling water solenoid valve 71 are composed; the pH automatic control system is composed of a pH reading device (not shown), a pH control unit (not shown), an acid solenoid valve 31 and a lye solenoid valve 41.

When applying the above-mentioned production system provided by this embodiment, first put the required materials into the reactor 1 through the feeding port 11, set the required temperature for the temperature control unit, set the required pH value for the pH control unit, and turn on Stirrer 15, temperature reading device, pH reading device and cooling water circulation device;

The thermometer 16 measures the temperature in the reactor 1 in real time. The temperature reading device reads the actual temperature value measured by the thermometer 16 and transmits it to the temperature control unit. The temperature control unit compares the actual temperature value with the set temperature value. If the actual temperature value is If the temperature is lower than the set temperature value, the temperature control unit turns on the hot steam circulation device 6 by opening the hot steam solenoid valve 61 to provide circulating hot steam to the jacket layer 14 to heat the reactor 1; When the temperature value is set, the temperature control unit closes the hot steam circulation device 6 by closing the hot steam solenoid valve 61 to stop heating the reactor 1; if the actual temperature value is higher than the set temperature value, the temperature control unit opens The cooling water solenoid valve 71 circulates cooling water to the jacket layer 14 to cool the reactor 1; when the actual temperature value is equal to the set temperature value, the temperature control unit stops the clamping by closing the cooling water solenoid valve 71 The jacket 14 conveys cooling water, thereby stopping the continued cooling of the reactor 1.

At the same time, the pH meter 17 measures the pH value in the reactor 1 in real time. The pH reading device reads the actual pH value measured by the pH meter 17 and transmits it to the pH control unit. The pH control unit compares the actual pH value with the set pH value. , If the actual pH value is higher than the set pH value, the pH control unit delivers acid liquid into the reactor 1 by opening the acid liquid solenoid valve 31; when the actual pH value is equal to the set pH value, the pH control unit closes The acid solenoid valve 31 stops sending the acid liquid into the reactor 1; if the actual pH value is lower than the set pH value, the pH control unit sends the lye into the reactor 1 by opening the lye solenoid valve 41; When the pH value is equal to the set pH value, the pH control unit stops delivering lye into the reactor 1 by closing the lye solenoid valve 41;

At the same time, the volatile solvent in the reactor 1 is heated and volatilized, and then enters the condenser 5 through the pipeline, and is cooled and then flows back to the reactor 1 through the pipeline to continue to participate in the reaction. Firstly, it can save the cost of solvent, and secondly, it can prevent the internal cause of the reactor 1. Too much gas is produced and the pressure rises.

At the same time, real-time samples are taken through the sampling port 13 and the extent of the reaction in the reaction kettle is detected by liquid chromatograph. After the reaction is complete, the materials are discharged into the centrifuge 8 through the discharge port 12 for solid-liquid separation treatment and separated. The liquid flows into the sewage treatment device 9 through the pipeline for purification treatment. After the centrifugation is completed, the separated solid materials are transferred to the washing tank 2 by driving, and after adding water, the agitator 22 is turned on for mixing, and after the mixing is uniform, it passes through the discharge port 21 is discharged into the centrifuge 8 for solid-liquid separation treatment. The separated liquid flows into the sewage treatment device 9 through the pipeline for purification treatment. After the centrifugation is completed, the separated solid materials are transferred to the vacuum dryer for drying treatment by driving. , That is, Rh2 products.

Claims

The Rh2 enzymatic large-scale production system is characterized in that the production system includes:

A reactor, the reactor is provided with a feeding port, a discharging port, a thermometer for measuring the temperature in the kettle, a pH meter for measuring the pH value in the kettle, and a stirring device for stirring the materials in the kettle, so The outer wall of the reactor is provided with a jacket layer for the circulation of cooling water and hot steam;

A cooling water circulation device, which is connected to the jacket layer of the reaction kettle through a pipeline, and is used to provide cooling water for the jacket layer of the reaction kettle to lower the temperature in the kettle;

A hot steam circulation device, which is connected to the jacket layer of the reaction kettle through a pipeline, and is used to provide the jacket layer of the reaction kettle with hot steam for raising the temperature in the kettle;

An acid storage tank, which is connected to the inside of the reaction kettle through a pipeline;

An alkali storage tank, which is connected to the inside of the reaction kettle through a pipeline;

A detection device for detecting the degree of reaction in the reaction kettle;

A primary solid-liquid separation device, the primary solid-liquid separation device is arranged below the discharge port of the reaction kettle, and is used to perform solid-liquid separation treatment on the materials coming out of the reaction kettle;

The water washing tank includes a tank body and a stirring device for stirring the materials in the tank body, and the water washing tank is used for cleaning the materials after a solid-liquid separation;

A secondary solid-liquid separation device, the secondary solid-liquid separation device is arranged below the discharge port of the water washing tank, and is used to perform solid-liquid separation treatment on the material coming out of the water washing tank;

Drying device is used to dry the material after the secondary solid-liquid separation.
The Rh2 enzymatic large-scale production system according to claim 1, wherein the acid storage tank and the alkali storage tank are arranged above the reaction kettle.
The Rh2 enzymatic large-scale production system according to claim 1, characterized in that: the production system further comprises a condensation device for cooling the solvent volatilized by heating in the kettle of the reaction kettle, and the condensation device is provided with It is located above the reaction kettle and communicates with the reaction kettle through a pipe to form a channel for the cooled solvent to flow back into the kettle.
The Rh2 enzymatic large-scale production system according to claim 1, wherein the production system further includes a transfer device for transferring materials.
The Rh2 enzymatic large-scale production system according to claim 4, wherein the transfer device is a crane.
The Rh2 enzymatic large-scale production system according to claim 1, characterized in that: the production system further comprises an automatic control system, and the automatic control system comprises a temperature for automatically controlling the temperature rise and fall in the reactor of the reactor. An automatic control system and a pH automatic control system for automatically controlling the increase and decrease of the pH value in the reactor of the reaction kettle.
The Rh2 enzymatic large-scale production system according to claim 6, wherein the temperature automatic control system includes a temperature reading device, a temperature control unit, and a cooling water solenoid valve for controlling the opening and closing of the cooling water circulation device , And a hot steam solenoid valve for controlling the opening and closing of the hot steam circulation device; one end of the temperature reading device is electrically connected to the thermometer, the other end is electrically connected to the temperature control unit, and the temperature control The unit controls the opening and closing of the cooling water solenoid valve and the hot steam solenoid valve respectively.
The Rh2 enzymatic large-scale production system according to claim 6, characterized in that: the pH automatic control system includes a pH reading device, a pH control unit, for controlling the acid liquid in the acid storage tank to the reaction An acid solenoid valve for conveying and stopping in the kettle, and an lye solenoid valve for controlling the conveying and stopping of the lye in the alkali storage tank into the reaction kettle; one end of the pH reading device is connected to the The pH meter is electrically connected, and the other end is electrically connected to the pH control unit. The acid solenoid valve is arranged on the pipe connecting the acid storage tank and the reaction kettle and is close to one end of the reaction kettle. The lye solenoid valve is arranged on the pipe connecting the alkali storage tank and the reaction kettle and is close to one end of the reaction kettle, and the pH control unit controls the acid solenoid valve and the lye solenoid valve respectively Opening and closing.
The Rh2 enzymatic large-scale production system according to any one of claims 1 to 8, wherein the production system further comprises a sewage treatment system for purifying liquid after solid-liquid separation treatment.