WO2020218840A1

WO2020218840A1 - Composition for inhibiting hair loss or promoting hair growth containing plant extract removed of chlorophyll or pigment as active ingredient, and method for producing same

Info

Publication number: WO2020218840A1
Application number: PCT/KR2020/005359
Authority: WO
Inventors: 배용태
Original assignee: 배용태; 주식회사 비엔티
Priority date: 2019-04-23
Filing date: 2020-04-23
Publication date: 2020-10-29
Also published as: KR20200124347A; KR102200906B1

Abstract

The present invention relates to a composition which is for inhibiting hair loss or promoting hair growth, includes Polygonum cuspidatum extract, Camellia japonica leaf extract, and Artemisia annua extract as active ingredients, and further contains at least one extract selected from the group consisting of Camellia sinensis leaf extract, Chamaecyparis obtusa leaf extract, and Houttuynia cordata leaf extract. The composition has an excellent hair loss-inhibiting or hair growth-promoting effect, is highly safe, and has no side effects, and thus the extract can be effectively used as a composition for inhibiting hair loss or promoting hair growth.

Description

Composition for inhibiting hair loss or promoting hair growth containing plant extracts from which chlorophyll or pigment has been removed as an active ingredient, and method for preparing the same

The present invention contains chlorophyll or pigment-removed polygonum cuspidatum extract, Camellia japonica leaf extract, and Artemisia annua extract as active ingredients, and green tea ( Camellia sinensis ) leaf extract, cypress Tree (Chamaecyparis obtusa) leaf extract and Eoseongcho ( Houttuynia cordata ) It relates to a composition for inhibiting hair loss or promoting hair growth further containing one or more extracts selected from the group consisting of leaf extract, and a method for preparing the same.

The scalp is composed of epidermis, dermis, and subcutaneous tissue, and the outermost tissue, the epidermis, functions to protect sensitive tissues of the body from damage. However, the scalp function may be lost due to environmental factors affecting the scalp, such as stress and lack of influence due to external stimuli such as temperature change, humidity change, exposure to pollutants, etc., and may affect hair loss. .

Tissues associated with hair loss include sebaceous gland, keratinocytes of the epidermis and hair follicles, dermal papilla cells, sweat gland, and root sheath of the scalp hair follicle. have.

In the expression of men, it is known that testosterone is involved in skeletal muscle growth and sperm formation, and dihydrotestosterone (DHT) is involved in acne, sebum increase, hair loss, and prostatic hypertrophy in the tissues ( Thiboutot et al., J. Invest.Dermatol ., 105(2):2019-14, 1995).

Androgenetic alopecia is a phenomenon caused by a male hormone called testosterone. When this testosterone is converted into dihydrotestosterone, a more powerful hormone by an enzyme called 5α-reductase, this hormone acts on the hair follicles and It induces hair loss from the growth stage to the degenerative stage. Therefore, a method of inhibiting the production of DHT by 5-alpha-reductase is mainly used to treat androgenetic alopecia.

Meanwhile, finasteride, a 5-alpha-reductase inhibitor, has been found to improve hair loss by reducing the hair loss hormone DHT in hair loss patients (Van Neste et al., 2000), through which 5-alpha-reductase has been found. Tase is an important target for the treatment of hair loss and is used in the development of a hair loss treatment, and it is a pharmacologically active substance that treats alopecia by inhibiting the activity of 5-alpha-reductase, a biological enzyme involved in the reaction of dihydrotestosterone from testosterone. There is a lot of research to find.

Gynecomastia occurs primarily due to a decrease in the amount of estrogen after menopause. Minoxidil or estrogen is mainly used as a treatment for gynecomastia. Alopecia areata is caused by autoimmune diseases, mental stress, or genetic predisposition. Such alopecia areata has a fundamentally different cause from androgenetic alopecia, and treatment is also different, so a method of treating adrenocorticosteroids, applying minoxidil to the affected area, or artificially inducing irritation to the affected area is used.

For such complex causes of hair loss, components aimed at promoting blood circulation, inhibiting male hormone action, and strengthening hair root function are sold as products on the market, but there is no clear effect yet, and there is a problem of side effects. May be raised. For example, in the case of minoxidil, side effects that cause sticky feeling and skin irritation have been reported, and finasteride is currently used as a formulation for oral administration, but side effects such as sexual dysfunction have been reported according to its intake. Since the effect can be expected only by oral administration, there were many inconveniences in use.

For the prevention and treatment of such hair loss, hormone replacement therapy has been used as the most effective method. However, when a hormone such as estrogen is administered for a long time, there is a risk of cancer such as breast cancer and uterine cancer, and side effects such as headache and weight gain occur.

Accordingly, the present inventors were studying plant extracts having excellent safety and no side effects while inhibiting hair loss or promoting hair growth, and extracts of Hojanggeun, Camellia leaf, Mugwort, Green tea leaf, Cypress leaf and Eoseongcho leaf It was confirmed that there is an effect of inhibiting hair loss or promoting hair growth, and thus the present invention was completed.

An object of the present invention is to provide a composition for inhibiting hair loss or promoting hair growth, and a method for producing the same, which is excellent in inhibiting hair loss or promoting hair growth, and has high safety and no side effects.

Another object of the present invention is to provide a method for inhibiting hair loss or promoting hair growth, comprising administering to an individual an extract having high safety and no side effects while having excellent hair loss inhibition or hair growth promoting effect of the present invention.

Another object of the present invention is to provide a use of an extract having high safety and no side effects while having excellent hair loss inhibition or hair growth promoting effect of the present invention for use as a composition for inhibiting hair loss or promoting hair growth.

In order to achieve the above object, the present invention No. muscle (Polygonum cuspidatum) extract, camellia (Camellia japonica) leaf extract, and firefly wormwood (Artemisia annua) containing the extract as an active ingredient, and green tea (Camellia sinensis) leaf extract, Cypress tree (Chamaecyparis obtusa) leaf extract and Eoseongcho ( Houttuynia cordata ) provides a composition for inhibiting hair loss or promoting hair growth, and a method for preparing the same, further containing at least one extract selected from the group consisting of leaf extract.

In addition, the present invention provides a functional cosmetic composition for inhibiting hair loss or promoting hair growth, containing the extract as an active ingredient.

In addition, the present invention provides a quasi-drug composition for inhibiting hair loss or promoting hair growth, containing the extract as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for inhibiting hair loss or promoting hair growth, containing the extract as an active ingredient.

In addition, the present invention provides a health food for inhibiting hair loss or promoting hair growth, containing the extract as an active ingredient.

In addition, the present invention provides a method for inhibiting hair loss or promoting hair growth, comprising administering to an individual a pharmaceutically effective amount of the extract.

In addition, the present invention provides the use of the extract for use as a composition for inhibiting hair loss or promoting hair growth.

In addition, the present invention provides the use of the extract for use as a functional cosmetic composition for inhibiting hair loss or promoting hair growth.

In addition, the present invention provides the use of the extract for use as a quasi-drug composition for inhibiting hair loss or promoting hair growth.

In addition, the present invention provides the use of the extract for use as a pharmaceutical composition for inhibiting hair loss or promoting hair growth.

In addition, the present invention provides the use of the extract for use as a health food for inhibiting hair loss or promoting hair growth.

Polygonum cuspidatum extract according to the present invention, Camellia japonica leaf extract, and artemisia annua extract as active ingredients, green tea ( Camellia sinensis ) leaf extract, cypress tree (Chamaecyparis obtusa) The composition for inhibiting hair loss or promoting hair growth further containing one or more extracts selected from the group consisting of leaf extract and Houttuynia cordata leaf extract is excellent in inhibiting hair loss or promoting hair growth, and at the same time has high safety and no side effects. The extract can be usefully used as a composition for inhibiting hair loss or promoting hair growth.

1 is a diagram showing a process of removing chlorophyll and pigment from a plant extract.

Figure 2 is a diagram showing the extract prepared in Example <3-1> from which the pigment was removed.

3 is a view showing a camellia leaf extract from which chlorophyll has been removed prepared according to Example <3-2>.

Figure 4 is a diagram showing a wormwood extract prepared according to Example <3-3> from which chlorophyll was removed.

5 is a diagram showing a green tea leaf extract from which chlorophyll is removed prepared according to Example <3-4>.

6 is a view showing a cypress leaf extract from which chlorophyll was removed prepared according to Example <3-5>.

7 is a diagram showing a leaf extract of Eoseongcho from which chlorophyll was removed prepared according to Example <3-6>.

8 is a diagram showing a composition for inhibiting hair loss or promoting hair growth in a mist form prepared according to <Preparation Example 1>.

9 is a diagram showing a composition for inhibiting hair loss or promoting hair growth in the form of a shampoo prepared according to <Preparation Example 2>.

FIG. 10 shows new hair on the crown, which is a wide range of circular hair loss sites of User 1, who used the mist and shampoo prepared according to <Preparation Example 1> and <Preparation Example 2> for a total of 3 years from March 2016 to March 2019. Is a diagram showing the generated state.

11 is a diagram showing the hair condition of User 2, who used the shampoo prepared according to <Preparation Example 1> and <Preparation Example 2> for a total of 16 months from February 2017 to June 2018.

FIG. 12 is a diagram showing a state in which hair that was not generated on the right forehead of User 3, who used the mist prepared according to <Production Example 1> for 14 months, was generated.

FIG. 13 is a diagram showing a state in which hair that was not generated on the left forehead of User 3, who used the mist prepared according to <Production Example 1> for 14 months, was generated.

14 is a diagram showing a state in which new hair is generated in a wide range of hair loss sites of User 4, who has used the shampoo prepared according to <Production Example 2> for about 40 days.

15 is a diagram showing a state in which new hair is generated in a wide range of hair loss sites of User 5, who has used the shampoo prepared according to <Production Example 2> for about 40 days.

16 is a diagram showing the pores of User 6 using the shampoo prepared according to <Preparation Example 2>.

17 is a diagram showing the pores of User 7 who used the shampoo prepared according to <Production Example 2>.

Hereinafter, the present invention will be described in detail.

The present invention comprises a polygonum cuspidatum extract, Camellia japonica leaf extract, and artemisia annua extract as active ingredients, green tea ( Camellia sinensis ) leaf extract, cypress tree ( Chamaecyparis obtusa ) leaf It provides a composition for inhibiting hair loss or promoting hair growth further containing one or more extracts selected from the group consisting of extracts and Eoseongcho ( Houttuynia cordata ) leaf extracts.

The extract is chlorophyll extracted by mixing with an extraction solvent consisting of at least one floral water selected from the group consisting of floral water extracted from Maengjongjuk leaves, floral water extracted from Sinuidae leaves, and floral water extracted from cypress leaves, and fermented alcohol. Or it may be a hair loss inhibiting or hair growth promoting species composition, characterized in that the extract from which the pigment has been removed, but is not limited thereto.

The chlorophyll extract has been removed from the pigment, and the camellia leaf extract, mugwort extract, green tea leaf extract, cypress leaf extract, and Eoseongcho leaf extract have chlorophyll removed.

The chlorophyll or pigment-removed hojanggeun extract, camellia leaf extract, wormwood extract, green tea leaf extract, cypress leaf extract and Eoseongcho leaf extract are preferably prepared by the following steps, but are not limited thereto.

1) adding an extraction solvent to the leaves of Hojanggeun, Camellia leaves, Mugwort, green tea leaves, Cypress leaves or Eoseongcho leaves, respectively, and extracting them;

2) cooling the extract of step 1) and filtering;

3) concentrating the filtered extract of step 2) under reduced pressure; And

4) It is selected from the group consisting of floral water extracted from wormwood, floral water extracted from green tea leaves, floral water extracted from camellia leaves, floral water extracted from Maengjongjuk, floral water extracted from Shin Medical University, and floral water extracted from cypress leaves. After any one or more of the floral water is mixed with the alcohol, it is added to the vacuum-concentrated extract in step 3), or any one or more floral water selected from the group consisting of the floral water is added to the vacuum-concentrated extract in step 3). Adding to remove chlorophyll or pigment.

In the above method, the leaves of the hawthorn, camellia leaves, wormwood wormwood, green tea leaves, cypress leaves or Eoseongcho leaves of step 1) can be used without limitation, such as those grown or commercially available.

The extraction solvent of step 1) is preferably water, alcohol, or a mixture thereof, but is not limited thereto. It is preferable to use a lower alcohol of C ₁ to C _{2 as} the alcohol, and it is preferable to use ethanol or methanol as the lower alcohol, but is not limited thereto. The extraction method is preferably shaking extraction or reflux extraction, but is not limited thereto. It is preferable to extract by adding the extraction solvent 2 to 10 times to the amount of green tea leaves, camellia leaves, or gooseberries, but is not limited thereto. The extraction temperature is preferably from room temperature to 50°C, but is not limited thereto. In addition, the extraction time is preferably 2 to 5 days, but is not limited thereto. In addition, the number of extractions is preferably 1 to 3 times, but is not limited thereto.

In the above method, the vacuum concentration in step 3) is preferably a vacuum vacuum concentrator or a vacuum rotary evaporator, but is not limited thereto.

The floral water extracted from the wormwood in step 4), the floral water extracted from the green tea leaves, the floral water extracted from the camellia leaves, the floral water extracted from Maengjongjuk, the floral water extracted from Shin Medical University or the floral water extracted from cypress leaves , Green tea leaves, camellia leaves, Maengjongjuk, Sinuidae or cypress leaves are extracted for 8 hours or more with an essential oil extractor, respectively, to prepare a plant liquid extract for removing chlorophyll and pigment, but is not limited thereto.

The floral water extracted from the wormwood in step 4), the floral water extracted from the green tea leaves, the floral water extracted from the camellia leaves, the floral water extracted from Maengjongjuk, the floral water extracted from Shin Medical University, or the floral water extracted from cypress leaves were mixed with ethanol. After mixing in a ratio of 9:1 to 7:3, it was diluted by adding 20 to 25 times to the concentrated extract of step 3), and then allowed to stand for 10 to 14 hours in freezing conditions at -40 to -60°C to precipitate It is preferable to separate the chlorophyll or pigment, but is not limited thereto. The method is a method of extracting water-soluble and fat-soluble plant extracts from which chlorophyll or pigment has been removed.

In addition, only floral water extracted from wormwood, floral water extracted from green tea leaves, floral water extracted from camellia leaves, floral water extracted from Maengjongjuk, floral water extracted from Shin Medical University or floral water extracted from cypress leaves are used as extraction solvents. It is preferable to separate the precipitated chlorophyll or pigment by adding 20 to 25 times to the concentrated extract of step 3), diluting it, and then allowing it to stand for 10 to 14 hours in freezing conditions at -40 to -60°C, but not limited thereto. Does not. The method is a method of extracting a water-soluble plant extract from which chlorophyll or pigment has been removed.

It is preferable to prepare a colorant-removed hojanggeun extract by mixing the extract with floral water extracted from wormwood, floral water extracted from Maengjongjuk, floral water extracted from Shin Medical University, and floral water extracted from cypress leaves. It is not limited.

It is preferable to prepare a camellia leaf extract from which chlorophyll has been removed by mixing the camellia leaf extract with floral water extracted from wormwood, floral water extracted from camellia leaves, floral water extracted from Maengjongjuk, and floral water extracted from cypress leaves. However, it is not limited thereto.

The wormwood extract is preferably mixed with floral water extracted from wormwood, floral water extracted from Maengjongjuk, floral water extracted from Shin Medical University, and floral water extracted from cypress leaves to prepare a wormwood extract from which chlorophyll is removed. It is not limited.

The green tea leaf extract is preferably mixed with floral water extracted from wormwood, floral water extracted from Maengjongjuk, floral water extracted from Shin Medical University, and floral water extracted from cypress leaves to prepare a green tea leaf extract from which chlorophyll has been removed. It is not limited.

It is preferable to prepare a cypress leaf extract from which chlorophyll is removed by mixing the cypress tree leaf extract with floral water extracted from wormwood, floral water extracted from Maengjongjuk, floral water extracted from Shin Medical University, and floral water extracted from cypress leaves. , But is not limited thereto.

The Eoseongcho leaf extract is preferably mixed with floral water extracted from wormwood wormwood, floral water extracted from Maengjongjuk, floral water extracted from Sinuidae, and floral water extracted from cypress leaves to prepare Eoseongcho leaf extract from which chlorophyll has been removed. It is not limited.

When manufacturing cosmetics using a functional cosmetic composition for inhibiting hair loss or promoting hair growth containing the extract as an active ingredient, the concentration of the plant extract from which the chlorophyll or pigment of the present invention is removed is 0.01 to 5 mg/ml.

The cosmetic composition may be, for example, a solution, a gel, an anhydrous product of a solid or dough, an emulsion obtained by dispersing an oil phase in an aqueous phase, a suspension, a microemulsion, a microcapsule, a microgranular sphere or an ionic (liposome), a nonionic vesicle dispersant It may be provided in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. In addition, it may be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition is a fatty substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent in addition to the plant extract from which the chlorophyll or pigment of the present invention is removed. , Fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles Or it may contain adjuvants commonly used in the field of cosmetics, such as any other ingredients commonly used in cosmetics.

It may be a quasi-drug composition for inhibiting hair loss or promoting hair growth containing the extract as an active ingredient, but is not limited thereto.

The term "quasi-drug" used in the present invention refers to items that are not instruments, machines, or devices among items used for the purpose of diagnosing, treating, improving, reducing, treating or preventing diseases of humans or animals, and structures and functions of humans or animals. It refers to items other than instruments, machines, or devices used for the purpose of having a pharmacological effect on the skin, and includes skin preparations and personal hygiene products.

When the quasi-drug composition is included in a quasi-drug for the purpose of preventing or improving hair loss, promoting hair growth or hair growth, the composition may be used as it is or may be used together with other quasi-drug ingredients, and may be appropriately used according to conventional methods. have. The mixing amount of the active ingredient can be appropriately adjusted according to the purpose of use.

In addition, the quasi-drug product of the present invention is not particularly limited thereto, but may be prepared and used in the form of, for example, a cream, lotion, aerosol, shampoo, gel or pack. Further, if necessary, a stabilizer, a preservative, an absorption accelerator, a pH adjuster, and other suitable additives may be blended.

It may be a pharmaceutical composition for inhibiting hair loss or promoting hair growth containing the extract as an active ingredient, but is not limited thereto.

The pharmaceutical composition may contain a pharmaceutically acceptable carrier. The composition comprising a pharmaceutically acceptable carrier may be in various oral or parenteral formulations. In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations contain at least one excipient, such as starch, calcium carbonate, sucrose or lactose, gelatin, etc. in one or more compounds. It is prepared by mixing. Further, in addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, and syrups.In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as humectants, sweeteners, fragrances, and preservatives may be included. have. Preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, and suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogelatin, and the like may be used.

The pharmaceutical composition is any selected from the group consisting of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, and suppositories. It can have one formulation.

In addition, the pharmaceutical composition is administered in a pharmaceutically effective amount. “Pharmaceutically effective amount” means an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type and severity of the subject, age, sex, activity of the drug, and Sensitivity, time of administration, route of administration and rate of excretion, duration of treatment, factors including co-used drugs and other factors well known in the medical field.

In addition, the pharmaceutical composition may be administered as an individual therapeutic agent or administered in combination with another therapeutic agent, and may be administered sequentially or simultaneously with a conventional therapeutic agent. And can be administered single or multiple. It is important to administer an amount capable of obtaining the maximum effect in a minimum amount without side effects in consideration of all the above factors, and can be easily determined by a person skilled in the art.

In addition, the route of administration of the pharmaceutical composition may be administered through any general route as long as it can reach the target tissue. As desired, intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, intranasal administration, intrapulmonary administration, or rectal administration may be performed, but are not limited thereto.

It may be a health food for inhibiting hair loss or promoting hair growth containing the extract as an active ingredient, but is not limited thereto.

There is no particular limitation on the type of food to which the chlorophyll or pigment-removed plant extract of the present invention is added. Examples of foods to which the above substances can be added include drinks, meat, sausages, bread, biscuits, rice cakes, chocolates, candies, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, There are beverages, alcoholic beverages and vitamin complexes, dairy products and dairy products, and all health functional foods in the usual sense are included.

The plant extract from which the chlorophyll or pigment of the present invention has been removed may be added to food as it is or may be used with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (for prevention or improvement). In general, the amount of the fermented red ginseng concentrate in the health functional food may be added in an amount of 0.1 to 90 parts by weight of the total food weight. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

When the health food composition according to the present invention is a beverage composition, there are no special restrictions on other ingredients other than those containing the extract as an essential ingredient in the indicated ratio, and various flavoring agents or natural carbohydrates, etc., as in ordinary beverages, are added as additional ingredients. It can be contained as. Examples of the above-described natural carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, and the like; And polysaccharides, for example, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 10 g per 100 g of the composition of the present invention.

In addition, the health food composition according to the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and heavy weight agents (cheese, chocolate, etc.), pectic acid and salts thereof, Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, and the like may be contained. In addition, natural fruit juice and fruit juice beverages, and may contain flesh for the production of vegetable beverages.

In addition, the present invention provides a method for preventing or improving hair loss, comprising administering to an individual a pharmaceutically effective amount of the extract.

In addition, the present invention provides a method for treating hair loss comprising administering to an individual a pharmaceutically effective amount of the extract.

The extract and hair loss are the same as the description of the composition for inhibiting hair loss or promoting hair growth, and the detailed description refers to the above.

On the other hand, using the chlorophyll and pigment removal plant floral water of the present invention, chlorophyll and pigments are separated as an active ingredient including a chlorophyll and a pigment extract, camellia leaf extract, and wormwood extract as active ingredients, green tea leaf extract, cypress leaf extract and A mixed extract further comprising one or more extracts selected from the group consisting of Eoseongcho leaf extract may be usefully used for inhibiting hair loss and promoting hair growth.

In a specific embodiment of the present invention, the present inventors use plant extracts such as Hojanggeun extract, camellia leaf extract, wormwood extract, green tea leaf extract, cypress leaf extract and Eoseongcho leaf extract and various chlorophyll and pigment removal plant floral water. As a result of confirming the optimal chlorophyll and pigment-removing plant floral water that significantly separates the chlorophyll and pigment of each extract, and removes the pigment from Hojanggeun extract, camellia leaf extract, wormwood extract, green tea leaf extract , Cypress tree leaf extract and Eoseongcho leaf extract were removed from chlorophyll.

In addition, the group consisting of a pigment-removed Hojanggeun extract prepared by the above method, a camellia leaf from which chlorophyll has been removed, and a wormwood extract as active ingredients, and a green tea leaf from which chlorophyll has been removed, a cypress leaf and Eoseongcho leaf extract Mist, shampoo and health food were prepared with a composition further containing one or more extracts selected from.

Hereinafter, the present invention will be described in detail by Examples, Experimental Examples and Preparation Examples.

However, the following Examples, Experimental Examples, and Preparation Examples are merely illustrative of the present invention, and the contents of the present invention are not limited to the following Examples, Experimental Examples, and Preparation Examples.

<실시예 1> 식물 추출물의 제조<Example 1> Preparation of plant extract

<1-1> 호장근 추출물의 제조<1-1> Preparation of Hojanggeun extract

Green tea leaf extract was prepared by a general procedure of extracting alcohol [fermented alcohol, Daehan Alcohol Life Co., Ltd.] from plants with a solvent.

Specifically, after containing 10 times alcohol in the washed and dried hojanggeun (500 g) supplied from Cheonil Pharmaceutical Co., Ltd., and immersed in it, and allowed to stand at room temperature for 72 hours, the broth pack [medium pack, 25 cm × 35 cm, 8P, TNC It was filtered so that particles less than 100 μm were passed through with [Eletronics Co., Ltd.]. In order to volatilize the alcohol, it was distilled using a vacuum concentrator (CCA-1111, EYELA4) at 50° C. for a certain period of time, and then concentrated to prepare an extract of Hojanggeun.

<1-2> 동백나무 잎 추출물의 제조<1-2> Preparation of Camellia Leaf Extract

Camellia leaf extract was prepared by extracting and concentrating the camellia leaf purchased from WelPito Co., Ltd. in the same manner as in the method for preparing the Hojanggeun extract of Example <1-1>.

<1-3> 개똥쑥 추출물의 제조<1-3> Preparation of wormwood extract

The wormwood wormwood supplied from the Gokseong farmhouse (Godal-myeon, Godal-myeon, Gokseong-gun, Jeollanam-do) was extracted and concentrated in the same manner as in Example <1-1> to prepare a wormwood extract.

<1-4> 녹차 잎 추출물의 제조<1-4> Preparation of green tea leaf extract

Green tea leaves supplied from Hadong Green Tea Research Institute were extracted and concentrated in the same manner as in Example <1-1>, to prepare a green tea leaf extract.

<1-5> 편백나무 잎 추출물의 제조<1-5> Preparation of cypress leaf extract

Cypress tree leaves supplied from the Jangheung Cypress Tree Complex were extracted and concentrated in the same manner as in the preparation method of the Hojanggeun extract of Example <1-1> to prepare cypress leaves extract.

<1-6> 어성초 잎 추출물의 제조<1-6> Preparation of Eoseongcho leaf extract

Eoseongcho leaves obtained from Gokseong farmhouse (Godal-myeon, Godal-myeon, Gokseong-gun, Jeollanam-do) were extracted and concentrated in the same manner as in Example <1-1>, to prepare Eoseongcho leaf extract.

<실시예 2> 엽록소 및 색소 제거용 식물 액상 추출물의 제조<Example 2> Preparation of liquid plant extract for removing chlorophyll and pigment

To prepare a plant liquid extract for removing chlorophyll and pigment, mugwort (7 kg), green tea leaves (7 kg), camellia leaves (7 kg), maengjongjuk leaves (6.5 kg), Sinuidae leaves (6.5 kg) and After purchasing cypress leaves (7 kg) on the spot, they were extracted for more than 8 hours with an essential oil extractor at Jeonnam Biological Industry Promotion Agency (Re) to prepare a liquid plant extract (Floral Water) for removing chlorophyll and pigment.

<실시예 3> 엽록소 및 색소가 제거된 식물 추출물의 제조<Example 3> Preparation of plant extract from which chlorophyll and pigment were removed

<3-1> 색소가 제거된 호장근 추출물의 제조<3-1> Preparation of the extract from which the pigment has been removed

The pigments in the extract of chlorophyll roots were removed using the liquid plant extract for removing chlorophyll and pigments prepared in <Example 2>.

Specifically, as shown in Fig. 1, after mixing the chlorophyll and the pigment-removing plant liquid extract (furoral water extracted from cypress leaves) prepared in Example 2 with ethanol in a ratio of 9:1, the < After diluting by adding 20 times to the extract prepared in Example 1>, it was allowed to stand sufficiently for about 12 hours in a freezing condition at -50°C.

As a result, since the pigment layer was precipitated and the active ingredient was separated into the upper layer, only the upper layer was harvested, thereby preparing the extract from which the pigment was removed. On the other hand, the extract from which the pigment was removed was completely frozen in a freezer and then freeze-dried with a freeze dryer (FDU-1200, EYELA) (FIG. 2).

<3-2> 엽록소가 제거된 동백나무 잎 추출물의 제조<3-2> Preparation of camellia leaf extract from which chlorophyll was removed

A camellia leaf extract from which chlorophyll was removed was prepared in the same manner as in Example <3-1> (FIG. 3).

<3-3> 엽록소가 제거된 개똥쑥 추출물의 제조<3-3> Preparation of chlorophyll-removed mugwort extract

A wormwood extract from which chlorophyll was removed was prepared in the same manner as in Example <3-1> (FIG. 4).

<3-4> 엽록소가 제거된 녹차 잎 추출물의 제조<3-4> Preparation of green tea leaf extract from which chlorophyll has been removed

A green tea leaf extract from which chlorophyll was removed was prepared in the same manner as in Example <3-1> (FIG. 5).

<3-5> 엽록소가 제거된 편백나무 잎 추출물의 제조<3-5> Preparation of cypress leaf extract from which chlorophyll was removed

Cypress tree leaf extract from which chlorophyll was removed was prepared in the same manner as in Example <3-1> (FIG. 6).

<3-6> 엽록소가 제거된 어성초 잎 추출물의 제조<3-6> Preparation of Eoseongcho leaf extract from which chlorophyll was removed

Eoseongcho leaf extract from which chlorophyll was removed was prepared in the same manner as in <3-1> in the above embodiment (FIG. 7).

<실험예 1> 식물 추출물의 최적 엽록소 또는 색소 제거용 식물 후로랄 워터 확인<Experimental Example 1> Identification of plant floral water for removing optimal chlorophyll or pigment from plant extracts

<1-1> 호장근 추출물의 최적 색소 제거용 식물 후로랄 워터 확인<1-1> Confirmation of plant floral water for optimal pigment removal of Hojanggeun extract

Among the various chlorophyll and pigment-removing plant floral waters prepared in <Example 2>, the optimum chlorophyll- and pigment-removing plant floral water was identified.

Specifically, chlorophyll and pigment removal were performed using the same method as in Example <3-1>.

As a result, as shown in Table 1 below, floral water extracted from wormwood, floral water extracted from Maengjongjuk, floral water extracted from Sinuidae, and floral water extracted from cypress leaves significantly separate the pigments of Hojanggeun extract. , It was confirmed that floral water extracted from green tea leaves, floral water and purified water extracted from camellia leaves could not separate the pigment (Table 1).

	개똥쑥에서 추출한 후로랄 워터Floral water extracted from wormwood	녹차 잎에서 추출한 후로랄 워터Floral water extracted from green tea leaves	동백나무 잎에서 추출한 후로랄 워터Floral water extracted from camellia leaves	맹종죽에서 추출한 후로랄 워터Floral water extracted from Maengjongjuk	신의대에서 추출한 후로랄 워터Floral water extracted from Shin Medical University	편백나무 잎에서 추출한 후로랄 워터Floral water extracted from cypress leaves	정제수Purified water
엽록소 분리/침전 여부Chlorophyll separation/precipitation	분리/침전 가능Separation/settling possible	분리가 어려움Difficult to separate	분리가 어려움Difficult to separate	분리/침전 가능Separation/settling possible	분리/침전 가능Separation/settling possible	분리/침전 가능Separation/settling possible	분리가 어려움Difficult to separate

<1-2> 동백나무 잎 추출물의 최적 엽록소 제거용 식물 후로랄 워터 확인<1-2> Confirmation of plant floral water for optimal chlorophyll removal of camellia leaf extract

Plant floral water for optimal chlorophyll removal of the camellia leaf extract was confirmed in the same manner as in Experimental Example <1-1>.

As a result, as shown in Table 2 below, floral water extracted from wormwood, floral water extracted from camellia leaves, floral water extracted from Maengjongjuk, and floral water extracted from cypress leaves significantly reduce chlorophyll from camellia leaf extract. However, it was confirmed that floral water extracted from green tea leaves, floral water and purified water extracted from Shin Medical University could not separate chlorophyll (Table 2).

	개똥쑥에서 추출한 후로랄 워터Floral water extracted from wormwood	녹차 잎에서 추출한 후로랄 워터Floral water extracted from green tea leaves	동백나무 잎에서 추출한 후로랄 워터Floral water extracted from camellia leaves	맹종죽에서 추출한 후로랄 워터Floral water extracted from Maengjongjuk	신의대에서 추출한 후로랄 워터Floral water extracted from Shin Medical University	편백나무 잎에서 추출한 후로랄 워터Floral water extracted from cypress leaves	정제수Purified water
엽록소 분리/침전 여부Chlorophyll separation/precipitation	분리/침전 가능Separation/settling possible	분리가 어려움Difficult to separate	분리/침전 가능Separation/settling possible	분리/침전 가능Separation/settling possible	분리가 어려움Difficult to separate	분리/침전 가능Separation/settling possible	분리가 어려움Difficult to separate

<1-3> 개똥쑥 추출물의 최적 엽록소 제거용 식물 후로랄 워터 확인<1-3> Confirmation of plant floral water for optimal chlorophyll removal of wormwood extract

In the same manner as in Experimental Example <1-1>, plant floral water for optimal chlorophyll removal of wormwood extract was confirmed.

As a result, as shown in Table 3 below, floral water extracted from wormwood, floral water extracted from Maengjongjuk, floral water extracted from Sinuidae, and floral water extracted from cypress leaves significantly separate the chlorophyll of wormwood extract. , It was confirmed that floral water extracted from green tea leaves, floral water extracted from camellia leaves, and purified water did not separate chlorophyll (Table 3).

<1-4> 녹차 잎 추출물의 최적 엽록소 제거용 식물 후로랄 워터 확인<1-4> Confirmation of plant floral water for optimal chlorophyll removal of green tea leaf extract

Plant floral water for optimal chlorophyll removal of green tea leaf extract was confirmed in the same manner as in Experimental Example <1-1>.

As a result, as shown in Table 4 below, floral water extracted from wormwood, floral water extracted from Maengjongjuk, floral water extracted from Sinuidae, and floral water extracted from cypress leaves significantly separate chlorophyll from green tea leaf extract. , It was confirmed that floral water extracted from green tea leaves, floral water extracted from camellia leaves, and purified water could not separate chlorophyll (Table 4).

<1-5> 편백나무 잎 추출물의 최적 엽록소 제거용 식물 후로랄 워터 확인<1-5> Identification of plant floral water for optimal chlorophyll removal from cypress leaf extract

Plant floral water for optimal chlorophyll removal of cypress leaf extract was confirmed in the same manner as in Experimental Example <1-1>.

As a result, as shown in Table 5 below, floral water extracted from wormwood, floral water extracted from Maengjongjuk, floral water extracted from Sinuidae, and floral water extracted from cypress leaves significantly separate chlorophyll from cypress leaf extract. Shikina, floral water extracted from green tea leaves, floral water extracted from camellia leaves, and purified water were found to be unable to separate chlorophyll (Table 5).

<1-6> 어성초 잎 추출물의 최적 엽록소 제거용 식물 후로랄 워터 확인<1-6> Confirmation of plant floral water for optimal chlorophyll removal from Eoseongcho leaf extract

In the same manner as in Experimental Example <1-6>, plant floral water for optimal chlorophyll removal of Eoseongcho extract was confirmed.

As a result, as shown in Table 6 below, floral water extracted from wormwood, floral water extracted from Maengjongjuk, floral water extracted from Sinuidae, and floral water extracted from cypress leaves significantly separate chlorophyll from Eoseongcho leaf extract. , It was confirmed that floral water extracted from green tea leaves, floral water extracted from camellia leaves, and purified water could not separate chlorophyll (Table 6).

<제조예 1> 미스트 형태의 탈모 억제 또는 발모 촉진용 조성물 제조<Production Example 1> Preparation of a composition for inhibiting hair loss or promoting hair growth in a mist form

Green tea containing as an active ingredient, a pigment-removed Hojanggeun extract, a camellia leaf from which chlorophyll was removed, and a wormwood extract prepared through the process of <Example 1> to <Example 3>, and additionally removed chlorophyll Contains a composition for inhibiting hair loss or promoting hair growth containing at least one extract selected from the group consisting of leaves, cypress leaves and Eoseongcho leaf extracts, to prepare a mist for inhibiting hair loss or promoting hair growth without any chemical components, In order to increase preservation, the ratio of purified water and floral water extracted from cypress leaves was maintained at 1:1 to 19 (FIG. 8).

The concentration of each extract is 0.01 to 5 mg/ml.

<제조예 2> 샴푸 형태의 탈모 억제 또는 발모 촉진용 조성물 제조<Production Example 2> Preparation of a shampoo-type composition for inhibiting hair loss or promoting hair growth

Green tea containing as an active ingredient, a pigment-removed Hojanggeun extract, a camellia leaf from which chlorophyll was removed, and a wormwood extract prepared through the process of <Example 1> to <Example 3>, and additionally removed chlorophyll A shampoo for inhibiting hair loss or promoting hair growth was prepared by mixing known shampoo ingredients in a composition for inhibiting hair loss or promoting hair growth containing one or more extracts selected from the group consisting of leaves, cypress leaves, and Eoseongcho leaf extracts (FIG. 9 ).

The concentration of each extract is 0.01 to 5 mg/ml.

<제조예 3> 건강식품 형태의 탈모 억제 또는 발모 촉진용 조성물 제조<Production Example 3> Preparation of a composition for inhibiting hair loss or promoting hair growth in the form of health food

<3-1> 밀가루 식품의 제조<3-1> Preparation of flour food

Green tea containing as an active ingredient, a pigment-removed Hojanggeun extract, a camellia leaf from which chlorophyll was removed, and a wormwood extract prepared through the process of <Example 1> to <Example 3>, and additionally removed chlorophyll A composition for inhibiting hair loss or promoting hair growth containing one or more extracts selected from the group consisting of leaves, cypress leaves and Eoseongcho leaf extracts is added to 0.5 to 5.0 parts by weight of wheat flour, and bread, cakes, cookies, Crackers and noodles were prepared.

<3-2> 스프 및 육즙(Gravies)의 제조<3-2> Preparation of soups and gravy

Green tea containing as an active ingredient, a pigment-removed Hojanggeun extract, a camellia leaf from which chlorophyll was removed, and a wormwood extract prepared through the process of <Example 1> to <Example 3>, and additionally removed chlorophyll 0.1 to 5.0 parts by weight of a composition for inhibiting hair loss or promoting hair growth containing one or more extracts selected from the group consisting of leaves, cypress leaves and Eoseongcho leaf extracts are added to soups and broth to promote health products, soup of noodles, and The juice was prepared.

<3-3> 그라운드 비프(Ground beef)의 제조<3-3> Preparation of ground beef

Green tea containing as an active ingredient, a pigment-removed Hojanggeun extract, a camellia leaf from which chlorophyll was removed, and a wormwood extract prepared through the process of <Example 1> to <Example 3>, and additionally removed chlorophyll A composition for inhibiting hair loss or promoting hair growth containing at least one extract selected from the group consisting of leaves, cypress leaves and Eoseongcho leaf extracts was added to 10 parts by weight of ground beef to prepare ground beef for health promotion.

<3-4> 유제품(Dairy products)의 제조<3-4> Manufacture of Dairy products

Green tea containing as an active ingredient, a pigment-removed Hojanggeun extract, a camellia leaf from which chlorophyll was removed, and a wormwood extract prepared through the process of <Example 1> to <Example 3>, and additionally removed chlorophyll A composition for inhibiting hair loss or promoting hair growth containing at least one extract selected from the group consisting of leaves, cypress leaves and Eoseongcho leaf extracts is added to 5 to 10 parts by weight of milk, and various such as butter and ice cream using the milk Dairy products were prepared.

<3-5> 선식의 제조<3-5> Manufacturing of Seonsik

Brown rice, barley, glutinous rice, and adlay were gelatinized by a known method, dried, and then roasted, and then prepared into a powder having a particle size of 60 mesh with a grinder.

Black soybeans, black sesame seeds, and perilla seeds were also steamed and dried by a known method, and then roasted, and then prepared into a powder having a particle size of 60 mesh with a grinder.

Green tea containing as an active ingredient, a pigment-removed Hojanggeun extract, a camellia leaf from which chlorophyll was removed, and a wormwood extract prepared through the process of <Example 1> to <Example 3>, and additionally removed chlorophyll A composition for inhibiting hair loss or promoting hair growth containing at least one extract selected from the group consisting of leaves, cypress leaves and Eoseongcho leaf extracts is concentrated under reduced pressure in a vacuum concentrator, and the dried product obtained by drying with spraying and hot air dryer is subjected to particle size 60 It was pulverized with a mesh to obtain a dry powder.

It was prepared by mixing the grains, seeds and extracts prepared above in the following ratio.

Grains (30 parts by weight of brown rice, 15 parts by weight of barley, 20 parts by weight of barley),

Seeds (perilla 7 parts by weight, black soybean 8 parts by weight, black sesame 7 parts by weight).

Extracts of the present invention (3 parts by weight),

Ganoderma lucidum (0.5 parts by weight),

Rehmannia (0.5 parts by weight)

<3-6> 건강음료의 제조<3-6> Manufacture of health drinks

Subsidiary materials such as liquid fructose 0.5%, oligosaccharide 2%, sugar 2%, salt 0.5%, water 75%, and the pigment of the present invention are removed, Camellia leaves and Mugwort extracts from which chlorophyll is removed as active ingredients Including, in addition, 0.5 g of a composition for inhibiting hair loss or promoting hair growth containing at least one extract selected from the group consisting of green tea leaves, cypress leaves, and Eoseongcho leaf extracts from which chlorophyll has been removed is homogeneously blended to perform instant sterilization. It was prepared by packaging it in a small container such as a glass bottle or a PET bottle.

<3-7> 야채 주스의 제조<3-7> Preparation of vegetable juice

Selected from the group consisting of the extract of the present invention from which the pigments have been removed, the Camellia leaves from which chlorophyll has been removed, and the Mugwort extract as active ingredients, and additionally from the group consisting of green tea leaves from which chlorophyll has been removed, cypress leaves and Eoseongcho leaves extract Vegetable juice was prepared by adding 0.5 g of a composition for inhibiting hair loss or promoting hair growth containing one or more extracts to be added to 1,000 ml of tomato or carrot juice.

<3-8> 과일 주스의 제조<3-8> Preparation of fruit juice

Selected from the group consisting of the extract of the present invention from which the pigments have been removed, the Camellia leaves from which chlorophyll has been removed, and the Mugwort extract as active ingredients, and additionally from the group consisting of green tea leaves from which chlorophyll has been removed, cypress leaves and Eoseongcho leaves extract A fruit juice was prepared by adding 0.1 g of a composition for inhibiting hair loss or promoting hair growth containing one or more extracts to be added to 1,000 ml of apple or grape juice.

The present invention No. muscle (Polygonum cuspidatum) extract, camellia (Camellia japonica) leaf extract, and firefly wormwood (Artemisia annua) containing the extract as an active ingredient, and green tea (Camellia sinensis) leaves extract, cypress in accordance with the present invention ( Chamaecyparis obtusa ) leaf extract and Houttuynia cordata ( Houttuynia cordata ) The composition for inhibiting hair loss or promoting hair growth further containing one or more extracts selected from the group consisting of leaf extract is excellent in inhibiting hair loss or promoting hair growth, and at the same time has high safety and side effects Therefore, the extract can be used as a composition for inhibiting hair loss or promoting hair growth.

Claims

Including polygonum cuspidatum extract, Camellia japonica leaf extract, and Artemisia annua extract as active ingredients,

Green tea ( Camellia sinensis ) leaf extract, cypress tree ( Chamaecyparis obtusa ) leaf extract and Eoseongcho ( Houttuynia cordata ) a composition for inhibiting hair loss or promoting hair growth further containing one or more extracts selected from the group consisting of leaf extracts.
The method of claim 1, wherein the extract,

Extracted by mixing with an extraction solvent consisting of at least one type of floral water and fermented alcohol selected from the group consisting of floral water extracted from Maengjongjuk leaves, floral water extracted from Sinuidae leaves, and floral water extracted from cypress leaves,

Composition for inhibiting hair loss or promoting hair growth, characterized in that the extract from which chlorophyll or pigment has been removed.
A functional cosmetic composition for inhibiting hair loss or promoting hair growth, comprising the extract of claim 1 or 2 as an active ingredient.
A quasi-drug composition for inhibiting hair loss or promoting hair growth, comprising the extract of claim 1 or 2 as an active ingredient.
A pharmaceutical composition for inhibiting hair loss or promoting hair growth, comprising the extract of claim 1 or 2 as an active ingredient.
Health food for inhibiting hair loss or promoting hair growth, containing the extract of claim 1 or 2 as an active ingredient.
A method for inhibiting hair loss or promoting hair growth, comprising administering to an individual the extract of claim 1 or 2 in a pharmaceutically effective amount.
Use of the extract of claim 1 or 2 for use as a composition for inhibiting hair loss or promoting hair growth.
Use of the extract of claim 1 or 2 for use as a functional cosmetic composition for inhibiting hair loss or promoting hair growth.
Use of the extract of claim 1 or 2 for use as a quasi-drug composition for inhibiting hair loss or promoting hair growth.
Use of the extract of claim 1 or 2 for use as a pharmaceutical composition for inhibiting hair loss or promoting hair growth.
Use of the extract of claim 1 or 2 for use as a health food for inhibiting hair loss or promoting hair growth.