WO2013019049A1 - Complex comprising steviol glycosides orlicorice root, and poorly soluble material - Google Patents

Abstract

The present invention relates to: a complex with increased solubility of a poorly soluble material, comprising steviol glycosides or stevia containing the same, or licorice root, and a poorly soluble material; a preparation method thereof; a pharmaceutical or food composition containing the complex; a cosmetic or feed composition containing the complex; a solubilizing agent for increasing the solubility of a poorly soluble material; and a method for solubilizing a poorly soluble material. Since the complex of the present invention is remarkably improved in the solubility of a poorly soluble material, the complex can be easily prepared into a dosage form to be administered orally and by injection, has excellent storage stability, and has the effects of remarkable drug delivery ability and absorption rate into the body.

Description

Complex comprising steviol glycosides or licorice and sparingly soluble substances

The present invention provides a poorly soluble substance-containing complex with increased solubility, a method for preparing the same, a pharmaceutical or food composition comprising the complex, a cosmetic composition or a feed composition comprising the complex, a solubilizer for increasing the solubility of the poorly soluble substance, and A method for solubilizing poorly soluble substances.

Solubilization of poorly soluble drugs is an essential technique for administering drugs into the body in oral and injectable form. As a method of solubilizing a poorly water-soluble drug, firstly, the drug is dissolved in a mixed solvent of a water-miscible organic solvent and water; Second, modifying the structural formula of the drug to form salts of acids or bases that are soluble in water; Third, combining the drug with a third material to form a complex that is soluble in water; Fourth, a method of micellizing a drug in an aqueous solution by adding a surfactant is used (Leon Lachman, "The Theory and Practice of Industrial Pharmacy", Lea & Febiger, Philadelphia, 1986).

 Meanwhile, as a representative formulation development company that develops solubilization technology of poorly soluble drugs in developed countries such as the US and Europe, Alza, Euland, Knoll, Gatteposse, R.P. Scherer, Ethypharm, Emisphere, etc. are mainly developing specialized solubilization technology products. For example, Alza has developed a technology called nanocrystals, which uses a special mill to break down the particles into nano units and uses new additives to prevent agglomeration between particles. It is applied to soluble drugs and immunosuppressive sirolimus.

Euland is researching various solubilization and absorption improvement agents. It is developing and applying to the product an amorphous technology called BIORISE and a technology to improve the absorption rate called Glytech. In particular, Glytech technology is a technology that can promote their absorption by using a taxane-based anticancer agent that is blocked by the P-glycoprotein in combination with a P-glycoprotein inhibitor.

Knoll, a subsidiary of Abbott, has developed a technology called Meltrex, which significantly improves bioavailability by melting poorly soluble drugs and substrates at high temperatures and then cooling them to amorphize them.

  Gatteffosse, France, has developed a microemulsion microfluidic particle control technology called SMEDDS (self-microemulsifying drug delivery system) and applied it to the product.The SMEDDS technology provides the optimum ratio of surfactants, co-surfactants and poorly soluble drugs. It is characterized by being mixed and solubilized and filled in a capsule.

In Korea, Hanmi Pharm is using microemulsion technology to increase the solubility of poorly soluble substances, and Yuhan Corp. and Dong-A Pharm have been successful in exporting technology by applying it to products such as cyclosporin A and itraconazole, which are immunosuppressants.

In addition, Paclitaxel, which has a market scale of 2 trillion won per year as a single anticancer drug source approved by the US Food and Drug Administration (FDA), is a poorly soluble substance and is not dispersed in distilled water at all. Because of the mixed use has the disadvantage of causing serious side effects to the human body. Therefore, in order to solve this problem, Chitolife Co., Ltd. and Biomedical Polymer Research Team of Sunchon National University prepared advanced chitosan, a non-toxic natural polymer material, and applied it to paclitaxel in 2010, using existing organic solvents. It is much safer than the preparation method, and has a very good redispersibility in distilled water, making the injection formulation much easier to use as an injection.

Meanwhile, among the solubilization methods of poorly soluble substances, a method of solubilizing without changing the chemical structure of a drug using a surfactant is widely used as a method for solubilizing various drugs. As the surfactant, nonionic surfactants such as sorbitan fatty acid ester derivatives (Tween) and polyoxyethylene monoalkyl ether derivatives (BRIJ and MYRJ series) are widely used. However, these surfactants are very limited in use due to side effects such as hypersensitivity reactions, there is a disadvantage that the drug is precipitated when left for a relatively long time due to the very low stability of micelles.

In accordance with the above problems of poorly soluble materials and the need for the development of technology for solubilizing poorly soluble materials, the present inventors are poorly soluble in a solution to which steviol glycoside or licorice extract is added in order to maximize the solubility of the poorly soluble materials. Not only did solubility increase by dissolving the substance, but also the solubility of the poorly soluble substance was significantly increased as a result of the microwave treatment after dissolving the poorly soluble substance in the solution to which the steviol glycoside or licorice extract was added. By confirming that the present invention was completed, the present invention was completed.

It is an object of the present invention to provide a poorly soluble material containing composite with increased solubility.

It is another object of the present invention to provide a pharmaceutical or food composition having increased absorption in the body including the complex.

Still another object of the present invention is to provide a cosmetic composition comprising the complex.

Another object of the present invention is to provide a feed composition comprising the complex.

Another object of the present invention relates to a method for producing the composite.

It is another object of the present invention to provide a solubilizer, which can solubilize poorly soluble materials.

It is another object of the present invention to provide a method for solubilizing poorly soluble materials.

Another object of the present invention is to provide the use of the solubilizer.

Steviol glycosides included in the complex of the present invention, stevia or licorice containing the same can be used as a solubilizer of a poorly soluble material, the formulation obtained by treating the complex containing the solubilizer with microwave solubility of the poorly soluble material This markedly elevated feature is that it is easy to prepare in oral and injectable formulations, has excellent storage stability, and has excellent drug delivery ability and absorption rate in the body.

Figure 1 shows the solubility of curcumin in each condition after dissolving curcumin in a solution containing stevioside at different temperatures.

Figure 2 shows the solubility of curcumin in each case by dissolving curcumin in a solution containing steviosides of different concentrations, followed by sonication, a wet sterilizer or a microwave.

Figure 3 shows the correlation of four factors by surface response analysis: a, microwave power-stevioside concentration; b, curcumin (KW-100) concentration-microwave power; c, reaction time-microwave power; d, curcumin concentration-stevioside concentration; e, reaction time-stevioside concentration; f, reaction time-curcumin concentration.

Figure 4 shows the solubility of curcumin in each case by dissolving curcumin in a solution containing licorice extract of varying concentrations, followed by sonication, wet sterilizer or microwave.

In order to achieve the above object, the present invention is a steviol glycoside or stevia, or licorice comprising the same; And a poorly soluble material.

In another aspect, the present invention provides a pharmaceutical or food composition comprising the complex.

As another aspect, the present invention provides a cosmetic composition comprising the complex.

In another aspect, the present invention provides a feed composition comprising the complex.

In another aspect, the present invention is a steviol glycoside or stevia, or licorice comprising the same, and the first step of mixing a poorly soluble material; And a second step of treating the mixture obtained in the first step with microwaves, thereby providing a method for producing a poorly soluble substance-containing composite having increased solubility.

In another aspect, the present invention provides a solubilizer containing licorice as an active ingredient.

In another aspect, the present invention provides a method of solubilizing poorly soluble material, comprising mixing licorice with poorly soluble material.

In another aspect, the present invention provides the use of licorice in the preparation of a solubilizer.

Hereinafter, the present invention will be described in detail.

The present invention is a steviol glycoside or stevia, or licorice comprising the same; And a poorly soluble material.

The present invention is characterized by using a steviol glycoside as a surfactant that is a solubilizer for poorly soluble substances.

In the embodiment of the present invention, when the poorly soluble substance is dissolved in a solution containing steviol glycosides, it was confirmed that the solubility of the poorly soluble substance is significantly increased than when dissolved in water, so that the solubility is increased according to the present invention The complex may comprise stevio glycoside as one component.

In the present invention, the term “steviol glycoside” is a compound present in the leaves of Stevia rebaudiana Bertoni, and means a substance that gives a sweet taste. The steviol glycosides are not limited thereto, but the aqueous extract obtained by extracting dried leaves with hot water is concentrated by treatment with an adsorbent resin, and then purified by recrystallization using methyl alcohol or ethyl alcohol, and then dried. And commercially available ones can be used.

The type of steviol glycoside is not limited, but stevioside, rebaudioside, rebaudioside A, rebaudioside C, and dulcoside A found in high content in stevia plants In addition, rebaudioside B, D, E, F, rubuososide, steviool biosides, steviol monosides and the like can be included, specific chemical structures are as follows.

Formula 1

In addition, the steviol glycosides of the present invention include enzymatically treated stevia (SWETA), SWETA ML01 containing 50% steviol glycoside, SWETA75 containing 75% steviol glycoside, and the like according to the content and type of steviol glycoside of the chemical structure. Can be included

The enzyme treatment stevioside refers to a form in which glucose is added to stevioside (ie, glucosyl stevioside) using a sugar transfer enzyme. The present invention may be prepared using, but not limited to, CGTase (cyclodextringlucanotransferase) or glucose transferase of various microbial origins.

In the present invention, steviol glycosides are preferably steviosides.

In addition, the present invention is to use stevia containing a high content of stevioside, rebaudioside A, rebaudioside C, dulcoside, etc. as a solubilizer for poorly soluble substances. It is characteristic.

Stevia is a perennial herbaceous perennial plant with dicotyledonous plants, lanterns, asteraceae, perennial herbaceous perennial herb, inhabiting borderlands and streams and wetlands around South America, Paraguay, Argentina, and Brazil. Herbal tea, beverages, herbal preparations, natural sweeteners, diabetes, diet, and health supplements. Used for food.

In the present invention, stevia may be purchased and used commercially, or may be used collected or grown in nature.

 Stevia is preferably used in the present invention, but is not limited thereto.

The stevia is any species of the genus Stevia, such as Stevia rebaudiana, Stevia eupatoria, Stevia ovata, Stevia plummerae, Stevia salicifolia And Stevia serrata.

 Stevia in the present invention includes the plant itself or a pulverized product, an extract or fraction thereof.

In general, stevia may contain about 10 to 95%, preferably about 40 to 85% of steviosides and steviol.

In the present invention, the stevia extract can be obtained by extracting with water, a lower alcohol having 1 to 4 carbon atoms (C ₁ ~ C ₄ ) or a mixed solvent thereof. Specifically, about 2 to 10 times the weight of stevia, preferably 2 to 5 times the volume of lower alcohols such as water, ethanol and methanol, preferably ethanol 20 to 50 ° C, preferably 25 to 30 ° C After extraction was obtained by stirring extraction, ultrasonic extraction, continuous extraction 2-3 times by hot water extraction method at 100 ℃, filtered through filtrate and the filtrate was removed with a rotary depressurizer, the residue was vacuum lyophilized, hot air drying stevia extract Can be obtained.

In addition, the stevia extract of the present invention may include any one or more of an extract obtained by the extraction treatment, a dilution or concentrate of the extract, a dried product obtained by drying the extract, and these modifiers or purified products.

In addition, the present invention is characterized by using licorice as a solubilizer for poorly soluble substances. Licorice is the root of a plant in the genus Glycyrrhiza, which is known to stop coughing, reduce fever, relax the stomach and relieve an emergency. The main component of licorice extract is glycyrrhizinic acid, a white or colorless crystalline powder.

 Licorice in the present invention includes the plant itself or the pulverized product, extract or fraction thereof. Preferably it may be licorice extract.

The licorice extract may be prepared using conventional extraction methods in the art, such as ultrasonic extraction, filtration and reflux extraction. Preferably it may be a liquorice dry product obtained by crushing the removed liquorice debris by washing and drying of water, extracted with an alcohol or a mixed solvent of a carbon number of 1 to ₄ (C ₁ ~ C 4) extract, and more preferably It may be an extract extracted with C ₁ ~ C ₄ alcohol, most preferably may be an extract extracted with methanol or ethanol. At this time, the extraction solvent is preferably 2 to 20 times the dry weight of licorice. For example, the licorice dry matter is chopped, and then put into an extraction container, a lower alcohol of C ₁ to C ₄ or a mixed solvent thereof, preferably methanol or ethanol, and allowed to stand at room temperature for a certain period of time, followed by filtration to obtain an alcohol extract. . At this time, the extraction is preferably left for 1 week at room temperature, after which it may be further subjected to methods such as concentration or lyophilization. Alternatively, the complex of the present invention may be prepared by purchasing a licorice extract on the market.

In the embodiment of the present invention, when the poorly soluble substance was dissolved in the solvent containing licorice extract, it was confirmed that the solubility of the poorly soluble substance was significantly increased than when dissolved in water, licorice extract can be used as a solubilizer of poorly soluble substance I found it for the first time.

As used herein, the term “solubilization” refers to a phenomenon in which the solubility of a material that is less soluble in water increases due to the presence of a substance such as a surfactant. Solubilizing oil-soluble vitamins and hormones, such as water-soluble or mixed with fungicides such as phenols to increase the effect, methods for promoting emulsion polymerization are widely applied, etc. In the present invention, as a solubilizer, steviol glycosides or the like Use stevia, or licorice.

The complex of the present invention may be mixed with a steviol glycoside or stevia including the same or licorice with a poorly soluble material to form a complex having a structure in which the poorly soluble material is easily dissolved.

In addition, in the complex of the present invention, steviol glycosides, stevia or licorice containing a range of ratios are not limited, but steviol glycosides or stevia is contained in a concentration of 0.1 to 25% (w / v) (based on solids) Preferably, licorice is preferably included at a concentration of 0.1 to 30% (w / v) (based on solids).

More preferably, the steviol glycoside or stevia is preferably contained at 1 to 25% (w / v) (based on solids), and licorice is included at a concentration (based on solids) of 0.1 to 25% (w / v). It is desirable to be.

In addition, the complex of the present invention may be a formulation obtained by treating microwaves.

The term "microwave" in the present invention is an alternating signal between 300 MHz and 300 GHz in the frequency band or between 1 m and 1 mm in the case of wavelength.

In an embodiment of the present invention, when a poorly soluble substance is added to a solution in which stevio glycoside, stevia extract, or licorice extract is mixed, and a homogeneous mixed solution is prepared by sonication, further treatment with ultrasonic treatment is performed. As a result of measuring the solubility of the poorly soluble substance by dividing it into the case of microwave treatment and the case of microwave treatment, it was confirmed that the solubilization of the poorly soluble substance was significantly increased when the microwave treatment was performed.

After mixing the solubilizer and the poorly soluble material of the present invention, the solubility of the poorly soluble material was significantly increased when the microwaves were treated with a wet sterilizer, that is, when it was treated with a high temperature of about 121 ° C. By simply increasing the temperature it can be seen that the solubility is not increased. This is achieved by treating microwaves with a combination of steviol glycosides or licorice that acts as a surfactant and homogenized sparingly soluble material due to sonication, thereby forming a more detailed molecular structure by the instantaneous heat and the wavelength and / or frequency of microwaves alone. Is assumed to be solubilized.

Based on the above test results, in the present invention, the complex is preferably a steviol glycoside or a stevia including the same, or a liquor obtained by mixing the licorice and a poorly soluble substance, followed by sonication. More preferably, steviol glycosides or stevia or licorice comprising the same, and poorly soluble materials are mixed and sonicated, followed by treatment with a wet autoclave, microwave or a mixture thereof, most preferably. Preferably a formulation obtained by treating microwaves.

The method of treating the microwave is not limited thereto, but may be reacted to a microwave oven in operation (including general household or synthetic only). In addition, the microwave is preferably treated for 1 minute to 20 minutes at 600 W to 800 W in a general household microwave oven, and more preferably, 10 to 20 minutes at 650 W to 750 W, and even more. Preferably, the treatment is performed at 700 W for 15 minutes. In addition, the microwave can be treated for 1 minute to 100 minutes at 50 W to 300 W in a microwave for synthesis only, preferably 1 to 20 minutes to 140 W to 240 W. More preferably, the treatment is performed at 160W to 220W for 6 minutes to 12 minutes, and still more preferably at 180W to 200W for 7 minutes to 10 minutes. The solubilization of poorly soluble substances was enhanced in the case of using a synthetic microwave, in particular, when curcumin was dissolved in a solution containing stevioside at a concentration of 10 mg / ml, about 17,000. It was confirmed that curcumin is solubilized at a concentration of mg / L (Example 3).

The number of microwave treatments is not limited, but is preferably performed two or more times. In addition, it can be obtained by adding a step of cooling the complex between each reaction, the formulation can be obtained by the further step of sonicating before reacting in the microwave.

As used herein, the term “poorly soluble substance” refers to a substance having low solubility in water, and specifically, may be a substance having a solubility in water of 50 mg / ml or less. The poorly soluble substance may be, for example, a poorly soluble anticancer agent, an antibacterial agent, a steroid, an anti-inflammatory drug, a sex hormone, an immunosuppressant, an antiviral agent, an anesthetic agent, an antiemetic agent or an antihistamine, and more specifically, paclitaxel, paclitaxel Derivatives, taxotere, adriamycin, teniposide, etoposide, daunomycin, methotrexate, mitomycin C, mitomycin C, carmustine carmustine, busulfan, dactinomycin, lomustine, megestrol acetate, melphalan, mitoxantrone, indomethacin , Etodolac, ibuprofen, camptothecin, camptothecin, topotecan, aspirin, pyroxicam, cimetidine, estrogen, estrogen Prednisolone, cortisone, hydrocortisone, diflorasone, phenesterine, daunorubicin, mitotane, visadine, halo Nitrosoreas, anthrocyclines, elliptices, diazepam, omeprazole, methoxyfluorane, isofluorane, enfluoran (enfluorane, halotane, benzocaine, dantrolene, barbiturates, cyclosporin A, azathioprine, amphotericin B (amphotericin) B), nystatine, itraconazole, biphenyl dimethyl dicarboxylate (BDD), idebenone, piposulfan, danazole, hemoglobin ( hemoglobin), curcuminoid compound , Resveratrol (resveratrol), silico-glycidyl alginic acid will be selected from (glycyrrhizinic acid) and its derivatives from the group consisting of, but are not limited to. The curcuminoid-based compound includes curcumin, curcudemethoxycurcumin, and bisdemethoxycurcumin.

In an embodiment of the present invention, the curcuminoid compound is separated from the turmeric extract, and among them, curcumin having a low solubility (curcumin, solubility of about 11 mg / L) at a concentration of 10 mg / ml, various steviol glycosides It was dissolved in the solution containing. As a result, the solubilization of curcumin, which was hardly soluble in water, increased in the solution containing steviol glycosides, and the solubility in the solution containing stevioside was increased about 318 times compared to water. As a result of further treatment with the general household microwave, it was confirmed that curcumin dissolved to 3,500 mg / L (Example 3). In addition, after dissolving curcumin at a concentration of 10 mg / ml in a solution containing licorice extract, as a result of treating the general domestic microwave, it was confirmed that the curcumin is solubilized to 5,100 mg / L (Example 4). In addition, as a result of treating microwaves using about 189 W and about 8.9 minutes using a synthetic-only microwave, the solubilization of curcumin was possible up to 17,000 mg / L (Example 3). As compared with the degree of solubilization in water, about 1,545 times improved results were obtained.

In another embodiment of the present invention, 10 mg of resveratrol or glucosyl resveratrol was dissolved in a solution containing stevioside, and it was confirmed that 6,200 mg / L resveratrol and 8500 mg / L of glucosyl resveratrol were dissolved. As a result, it was confirmed that the solubility was increased to 9,400 mg / L for resveratrol and 14,300 mg / L for glucosyl resveratrol. In addition, when dissolved in a solution containing licorice extract, resveratrol was dissolved 8,600 mg / L, glucosyl resveratrol 10,500 mg / L, resveratrol 11,600 mg / L, glucosyl Resveratrol was confirmed to increase the solubility to 17,300 mg / L. The above results indicate that solubility was significantly increased in the group treated with nothing compared to 10 mg / L of resveratrol and 50 mg / L of glucosyl resveratrol (Example 5).

In another embodiment of the present invention, after dissolving glycyrrhizinic acid and paclitaxol, which are known as poorly soluble substances, in a solution containing stevioside or licorice extract, the result of microwave treatment results in gelation reaction in a stevioside or licorice extract of small concentration. This progressed rapidly, and it was confirmed that their solubility was also significantly increased (Examples 6 and 7).

In short, the present invention provides a new type of complex which significantly increases the solubility of poorly soluble materials.

In another aspect, the present invention relates to a pharmaceutical or food composition comprising the complex described above.

The complex is characterized in that it comprises a steviol glycoside or stevia, or licorice containing the same as a solubilizer of poorly soluble substances. The complex is a complex in which the solubility of the poorly soluble substance is significantly increased. When used in a pharmaceutical or food composition, the complex is easy to prepare in oral and injectable form, has excellent storage stability, and delivers drugs. It is characterized by excellent ability and absorption in the body.

The therapeutic disease of the pharmaceutical composition of the present invention may vary depending on the type of poorly soluble substance included in the complex, for example, if paclitaxol is included in the complex of the present invention as a poorly soluble substance, the composition may be used for anticancer use. That is, it may be used for the prevention or treatment of cancer, and if curcumin is included in the complex of the present invention as a poorly soluble substance, it may be used for antitumor or anti-inflammatory use, which is a medicinal use of curcumin.

In addition, the composition of the present invention may further comprise a pharmaceutically acceptable carrier. The composition comprising a pharmaceutically acceptable carrier may be in various oral or parenteral formulations. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which form at least one excipient such as starch, calcium carbonate, sucrose or lactose (at least one compound). lactose) and gelatin. In addition to simple excipients, lubricants such as magnesium stearate, talc and the like are also used. Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, and syrups, and various excipients such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin, may be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The pharmaceutical or food composition is selected from the group consisting of tablets, pills, powders, granules, capsules, suspensions, liquid solutions, emulsions, syrups, sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations and suppositories. It can have either formulation.

There is no particular limitation on the kind of food. Examples of the food to which the complex can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, drinks, Alcoholic beverages and vitamin complexes, and the like and include all of the health foods in the conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates, etc. as additional components, as in the general beverage. Natural carbohydrates described above are glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin, cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetening agent, natural sweetening agents such as tautin and stevia extract, synthetic sweetening agents such as saccharin and aspartame, and the like can be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, And a carbonation agent used for the carbonated beverage. The proportion of such additives is not critical but is usually selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention. In addition, the composition of the present invention may contain a flesh for preparing natural fruit juice, fruit juice beverage and vegetable beverage. The proportion of such pulp is not critical, but is generally selected in the range of 0.01 to 10 parts by weight per 100 parts by weight of the composition of the present invention. These components can be used independently or in combination.

 In another aspect, the present invention relates to a method for preventing or treating a disease by administering the complex as described above to a subject.

The complex is a complex in which the solubility of the poorly soluble substance is significantly increased, and the disease may vary depending on the type of the poorly soluble substance.

As used herein, the term "individual" means all animals including humans that may be infected with the disease, and by administering the complex described in the present invention to the individual, the disease can be effectively prevented and treated.

The route of administration of the complex can be administered via any general route as long as it can reach the target tissue. The complex of the present invention may be administered as desired, but is not limited to intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, intranasal administration, pulmonary administration, rectal administration. The complex may also be administered by any device in which the active agent may migrate to the target cell.

In another aspect, the present invention relates to a cosmetic composition comprising the complex described above.

The complex is characterized in that it comprises a steviol glycoside or stevia, or licorice containing the same as a solubilizer of poorly soluble substances. The complex is a complex in which the solubility of the poorly soluble substance is significantly increased, and when it is used in a cosmetic composition, it is easy to be formulated into various forms of cosmetics.

The use of the cosmetic composition of the present invention may vary depending on the kind of poorly soluble substance included in the complex. For example, if the poorly soluble material has a whitening purpose, the cosmetic composition may be used as a cosmetic composition for whitening.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component. When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan. When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used. When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In another aspect, the present invention relates to a feed composition comprising the complex described above.

The complex is characterized in that it comprises a steviol glycoside or stevia, or licorice containing the same as a solubilizer of poorly soluble substances.

The feed composition also includes the form of a feed additive.

The use of the feed composition of the present invention may vary depending on the type of poorly soluble material included in the complex. For example, when the poorly soluble substance is curcumin, according to the influenza virus treatment effect of curcumin, the feed composition may be used as a feed composition to improve influenza virus infection disease and have an anti-influenza effect.

The feed may be classified into various types according to nutritional value, main ingredient, distribution, moisture content, blending state and processing type, and the feed may be used as a feed, a rich feed, a supplementary feed, a protein feed, a starch feed, a fat feed or a fiber feed. Possible, but not limited to.

In addition, the feed composition may additionally contain a carrier that is acceptable to poultry and livestock. In the present invention, the feed can be added as it is or a known carrier, stabilizer and the like, and various nutrients such as vitamins, amino acids and minerals, antioxidants, antibiotics, antibacterial agents and other additives can be added as necessary. The shape may be in a suitable state such as powder, granules, pellets or suspension. In the case of supplying the feed composition of the present invention, poultry, livestock, and the like can be supplied alone or mixed with feed.

In another aspect, the present invention relates to a quasi-drug composition comprising the complex described above.

The complex is characterized in that it comprises a steviol glycoside or stevia, or licorice containing the same as a solubilizer of poorly soluble substances.

The use of the quasi-drug composition of the present invention may vary depending on the kind of poorly soluble substance included in the complex.

The type of quasi-drug is not limited thereto, but may be, for example, a disinfectant cleaner, a disinfectant, a shower foam, a gagreen, a wet tissue, a detergent soap, a shampoo, a hand wash, a humidifier filler, a mask, an ointment, or a filter filler.

In another aspect, the present invention is a steviol glycoside or stevia, or licorice comprising the same, and the first step of mixing a poorly soluble material; And a second step of subjecting the mixture obtained in the first step to microwaves (microwaves).

In the above production method, steviol glycosides, stevia, licorice and poorly soluble substances are the same as described above.

The steviol glycosides are stevioside, rebaudioside A, rebaudioside C, dulcoside A, which are found in high content in stevia plants, as well as rebaudioside B, D , E, rubisoside, steviol bioside, steviol monoside, SWETA, SWETA ML01, SWETA75 and the like can be included, but is not limited thereto.

As described above, the poorly soluble substance can be used without limitation as long as it is a substance having low solubility.

In the first step, the present invention is steviol glycosides or stevia, or licorice containing the same, and the poorly soluble substances are mixed in a solvent, or steviol glycosides or stevia, or licorice containing the same, poorly soluble in the solution The material can be added and mixed.

In addition, the steviol glycoside or stevia in the first step is preferably included in a concentration (solid basis) of 0.1 to 25% (w / v), the licorice is a concentration (solid content of 0.1 to 30% (w / v) It is preferable to include as a reference). In addition, the first step is preferably carried out at 60 ℃ to 80 ℃, more preferably at 65 ℃ to 75 ℃, even more preferably at 70 ℃.

In the second step of processing the microwave, the method of processing the microwave is not limited to this, but may be reacted to a microwave oven in operation (including general household or synthetic only). In addition, the microwave is preferably treated for 1 minute to 20 minutes at 600 W to 800 W in a general household microwave oven, and more preferably, 10 to 20 minutes at 650 W to 750 W, even more. Preferably, the treatment is performed at 700 W for 15 minutes. In addition, the microwave can be treated for 1 minute to 100 minutes at 50 W to 300 W in a microwave for synthesis only, preferably 1 to 20 minutes to 140 W to 240 W. More preferably, the treatment is carried out at 160W to 220W for 6 minutes to 12 minutes, and even more preferably at 180W to 200W for 7 minutes to 10 minutes. The number of microwave treatments is not limited, but is preferably performed two or more times. In addition, it can be obtained by adding a step of cooling the complex between each reaction, the formulation can be obtained by the further step of sonicating before reacting in the microwave.

In an embodiment of the present invention, after performing the first step of mixing the steviol glycosides or stevia extract or licorice extract containing the same, and the poorly soluble substance, the solubility of the poorly soluble substance was measured, The solubilization of poorly soluble substances was significantly increased compared to those dissolved in water. In addition, after performing the second step of treating the microwave, the solubility of the poorly soluble substance was measured, and when dissolved in water, after the first step after sonication or wet sterilizer treatment It can be seen that the solubility of the poorly soluble substance was significantly increased.

As another aspect, the present invention relates to a solubilizer containing licorice as an active ingredient.

In the embodiment of the present invention, when the poorly soluble substance was dissolved in the solution containing licorice extract, it was confirmed that the solubility of the poorly soluble substance is significantly increased compared to the case of dissolving the poorly soluble substance in water. Therefore, licorice comprising the form of the licorice extract of the present invention can be used as a solubilizer, which promotes solubilization of poorly soluble substances.

Licorice extract in the solubilizer is not limited thereto, but is preferably included in a concentration of 0.1 to 25% (w / v).

In another aspect, the present invention relates to a method of solubilizing poorly soluble materials, comprising mixing licorice with poorly soluble materials.

The licorice may be licorice extract.

The mixing of the licorice and the poorly soluble substance may be performed as a step of mixing the licorice extract and the poorly soluble substance by adding a poorly soluble substance to the solution containing licorice. Or by mixing licorice extract and poorly soluble material in a solvent.

When the licorice and the poorly soluble material are mixed as described above, the licorice may increase the solubility of the poorly soluble material and solubilize the poorly soluble material.

The method may further comprise treating the microwaves after mixing licorice and the poorly soluble material.

In another aspect, the present invention provides the use of licorice in the preparation of solubilizers.

The licorice is preferably licorice extract.

In an embodiment of the present invention, when the poorly soluble substance was dissolved in the solution containing licorice extract, it was confirmed that the solubility of the poorly soluble substance was significantly increased than when the poorly soluble substance was dissolved in the solution not containing licorice extract. Thus, licorice has a use that can be used to prepare solubilizers of poorly soluble materials.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Example 1 Preparation of Turmeric Extract and Curcumin

1-1. Preparation of Turmeric Extract

The turmeric used in the present embodiment is generally available in Chinese medicine or on the market, and is dried in the form of dried Root of Curcuma longa Linne and then dried to obtain powder of the present invention to efficiently obtain the extract of the present invention. It was used by grinding. 7.5 L of 100% ethanol (EtOH) was added to 1.6 kg of turmeric, and the mixture was left to stand at room temperature for 5 days, filtered through a filter paper, and concentrated to obtain turmeric ethanol extract (170 g).

1-2. Isolation and Purification of Turmeric Fractions and Curcuminoid Compounds from Turmeric Extracts

170 g of ethanol extract of turmeric obtained in Example 1-1 was added and suspended in 1 L of water. This was placed in a separatory funnel, and fractional extraction using n-hexane and ethyl acetate in order to obtain n-hexane soluble extract (23 g), ethyl acetate soluble extract (85 g) and water soluble extract (34 g).

85 g of the ethyl acetate soluble extract obtained above was subjected to silica gel column chromatography [silica gel 500 g, 70 to 230 mesh] using chloroform, methanol, and a mixed solvent thereof (80: 1 to 1: 1) as a mobile phase. 15 fractions (Fr.-1-15) were separated. The sixth fraction (Fr.-6, 16 g) was purified by silica gel column chromatography (30 g, 30%, n-hexane: ethyl acetate (20: 1 to 1: 1 (v / v)) as a mobile solvent. 230 to 400 mesh) was carried out to obtain five fractions (Fr.-6-1-5).

Among the fractions obtained by performing silica gel column chromatography on Fr.-6-2-3 fractions (11 g) with chloroform, methanol and their mixed solvents (80: 1 to 4: 1) as mobile phases. Development was carried out by preparative TLC ([n-hexane: ethylacetate = 4: 1 (v / v)) to give curcumin (8 g), and the eighth fraction (Fr.-8) , 14 g) was prepared using a mixed solvent of n-hexane: ethyl acetate (20: 1 to 1: 1 (v / v)) and chloroform: methanol (80: 1 to 20: 1 (v / v)) as a mobile phase. Silica gel column chromatography (30 g, 230-400 mesh) was repeatedly performed to obtain Demethoxycurcumin (0.4 g) and Bisdemethoxycurcumin (0.2 g).

Example 2: Preparation of Resveratrol and Glucosyl Resveratrol

Ho Jang Keun was washed with water, dried in the shade, and then powdered with Waring brand. 3.6 kg of powdered ephedra root was added to 20 L of ethanol, and extracted by cold extraction at room temperature for 7 days, followed by filtration under reduced pressure (Watman, USA). Then, the filtrate extract was removed from the ethanol solvent at room temperature with a vacuum rotary concentrator to obtain 146 g of Keunjangeun crude extract as a residue.

The crude extract was suspended in 1 L of water, mixed with the same amount of ethyl acetate, and fractionated. The procedure was repeated four times to obtain 4 L of ethyl acetate fraction. The ethyl acetate soluble fraction was concentrated under reduced pressure to obtain 80 g of an ethyl acetate soluble extract.

40 g of the ethyl acetate soluble extract obtained above was subjected to silica gel column chromatography using a step gradient solvent system consisting of chloroform: methanol = (100: 0 to 1: 1) to obtain resveratrol. 830 mg of chloroform: methanol = 20: 1 fractions were obtained.

The chloroform: methanol = 20: 1 fraction in the E. coli ethanol extract, ethyl acetate fraction, and ethyl acetate fraction column chromatography obtained above was analyzed using LC-MS to confirm the resveratrol content.

Others, glycyrrhizinic acid, glucosyl resveratrol and rebaudioside were purchased from Sigma, and paclitaxol was provided by Samyang Genex, Inc., stevioside and Licorice extract was purchased from Daepyeong Co., Ltd. was used in the experiment. In addition, the enzyme treatment stevia (SWETA) which is a steviol glycoside, SWETA75 containing 75% of steviol glycosides, and ML01 containing 50% of steviol glycosides were purchased from Cheminex.

Example 3: Solubilization of Curcumin Using Steviol Glycosides

10 mg / ml of curcumin prepared by the method of Example 1 was prepared for each 10% (w / v) steviol glycoside solution (SWETA, enzymatically treated stevioside, rebaudioside, SWETA ML01, SWETA75, stevioside). It melt | dissolved and it processed for 10 minutes using the ultrasonic wave. The amount of curcumin solubilized from each solvent was quantified using HPLC, and the results were as shown in Table 1 (degree of solubilization of curcumin according to the steviol glycoside component). Table 1 below shows the degree of solubilization of curcumin in the amount of mg / L.

Table 1

	water	SWETA	Enzyme Treatment Stevioside	Rebaudioside	SWETAML01	SWETA75	Stevioside
Molten amount (mg / L)	11	270	350	430	600	750	850

As shown in Table 1, the solubilization of curcumin was the best in the solution containing stevioside. Therefore, experiments were conducted under various conditions using stevioside, which is the best solubilizer of curcumin among steviol glycosides.

First, when curcumin was dissolved in a solution containing stevioside, the temperature was set to 30 ° C., 50 ° C., and 70 ° C., and it was confirmed at which temperature the solubilization of curcumin was the highest. As a result, curcumin was dissolved at 110 mg / L at 30 ° C, 170 mg / L at 50 ° C, and 390 mg / L at 70 ° C. That is, it was confirmed that the solubility of curcumin was most increased at 70 ℃ (Fig. 1).

In addition, 10 mg / ml of curcumin is dissolved in stevioside solution and sonicated, followed by sonication, autoclave (121 ° C., 15 psi, 15 minutes), or microwave for 15 minutes in a microwave oven at 700 W. Reaction) were treated for 15 minutes each. The degree of solubilization of curcumin was confirmed under the three conditions, and the results are shown in Table 2 below.

TABLE 2

Stevioside concentration,% (w / v)	Curcumin Solubilization (mg / L)
	Ultrasonic treatment	Wet Sterilizer	Microwave
0.5	48	57	120
2	90	100	300
5	300	500	1,500
10	850	1,300	3,500

As shown in Table 2, when treated with 10% (w / v) stevioside, the solubilization of curcumin was higher when treated with a 15-minute wet sterilizer than when treated with ultrasound, Molten amount improved 450 mg / mL when treated with a wet sterilizer. In addition, the microwave treatment significantly increased the solubilization of curcumin than the sonication or wet sterilizer, and about 2,650 mg / L of curcumin was more dissolved than sonication, so that the degree of solubility was weak. It was confirmed that it was improved 4.1 times (FIG. 2).

From the above results, it was found that dissolution of curcumin in the stevioside solution can increase the solubility of curcumin, and furthermore, if the microwave treatment step is further performed, the solubility of curcumin can be increased by about four times or more. In addition, the solubility was increased by about three times when the mixture of curcumin and stevioside was treated at a high temperature (121 ° C.) of the wet sterilizer. From this, the temperature was simply increased by microwave treatment. It was found that the solubility was not increased due to the increase.

In addition, solubilized curcumin was optimized using a synthetic-only microwave (CEM's Discover legacy systems model). Basic experiments show four factors: microwave power (50-200 W), stevioside concentration (50-200 mg / L), curcumin concentration (20-200 mg / L), and reaction time 1-10 minutes. Optimized by surface reaction analysis method at (Table 3).

TABLE 3

The results of analyzing the correlations under the two conditions were as shown in FIG. 3. As a result, the optimized equation obtained in the optimizer was as follows. y = 4.94 + 4.21x                 _One+3.42x                 ₂+1.33x                 ₃+1.29x                 ₄+1.88x                 _One                 x                 ₂+2.08x                 _One                 x                 ₃+0.40x                 _One                 x                 ₄+1.31x                 ₂                 x                 ₃+0.91x                 ₂                 x                 ₄+0.75x                 ₃                 x                 ₄0.16x                 _One                 ²+0.35x                 ₂                 ²+0.37x                 ₃                 ²1.55x                 ₄                 ²The microwave power was expected to solubilize 17,000 mg / L after 8.9 min of a solution containing 189 W, 195 mg / L stevioside, and 183 mg / L curcumin. In addition, three repeated experiments under the same conditions showed that 16,950 mg / L of curcumin was solubilized.

Example 4: Solubilization of Curcumin Using Licorice Extract

10 mg / ml of curcumin prepared by the method of Example 1 was dissolved for each 1-5% (w / v) licorice extract solution and treated with ultrasonic waves for 10 minutes. The amount of curcumin solubilized from each solvent was measured. Quantitation using HPLC, the results are shown in Table 4 below.

Table 4

	Concentration of Licorice Extract (%, w / v)
	1% (w / v) Licorice Extract	2% (w / v) Licorice Extract	3% (w / v) Licorice Extract	4% (w / v) Licorice Extract	5% (w / v) Licorice Extract
Molten amount (mg / L)	280	320	430	510	620

As shown in Table 4, the solubilization of curcumin was excellent at the concentration of licorice extract higher than the low concentration. In addition, 10 mg / ml curcumin was dissolved in 0.5, 2, 5, 10% (w / v) licorice extract solution and sonicated, followed by sonication, wet sterilizer (121 ° C., 15 psi, 15 minutes), microwave 15 minutes each. The concentration of licorice extract and the degree of solubilization of curcumin according to the three post-treatments are shown in Table 5 below.

Table 5

Concentration of licorice extract,% (w / v)	Curcumin Solubilization (mg / L)
	Ultrasonic treatment	Wet Sterilizer	Microwave
0.5	48	57	100
2	100	100	1,700
5	300	400	3,800
10	1200	1,500	5,100

As shown in Table 5, the solubilization of curcumin was higher with 15 minutes of wet sterilizer than with sonication, and the amount of curcumin improved about 300 mg / mL. In addition, the microwave treatment significantly increased the solubilization of curcumin than the sonication or wet sterilizer, and about 3,900 mg / L of curcumin was dissolved more so than sonication. It was confirmed that 4.16 times improved (FIG. 4). In addition, if microwave was used, curcumin (11 mg / L), which was hardly solubilized in water, dissolved about 100 mg / L in 0.5% (w / v) licorice extract solution and 10% (w / v) licorice. It was dissolved up to 5,100 mg / L in the extract solution. The above result is the highest degree of solubilization of curcumin so far known, which is about 463 times improved compared to the degree of solubilization in water.

Example 5: Solubilization of Resveratrol and Glucosyl Resveratrol Using Steviol Glycosides

Glucosyl resveratrol purchased from Sigma and 20 mg / ml of resveratrol prepared by the method of Example 2 were dissolved for each 0-20% (w / v) stevioside or licorice extract solution, and ultrasonically used at 70 ° C. Treated for 15 minutes. In addition, the microwaves were treated twice each for 15 minutes to quantify the amounts of resveratrol and glucosyl resveratrol solubilized from each solvent using HPLC. The results were compared with the case of only ultrasonic treatment without microwave treatment, and the results are shown in Table 6 below.

Table 6

Stevioside concentration,% (w / v)	Solubilized Resveratrol (mg / L)		Solubilized Glucosyl Resveratrol (mg / L)
	Ultrasonic treatment	Microwave	Ultrasonic treatment	Microwave
0	10	10	50	530
2.5	900	2,200	1,100	5,800
5	1,600	4,900	3,100	8,300
10	3,300	6,300	4,200	12,200
20	6,200	9,400	8,500	14,300

As shown in Table 6, the amount of resveratrol finally solubilized in the sample to which 20% (w / v) of stevioside was added was 6,200 mg / L, which was 10 mg / L solubilized resveratrol in a solution containing no stevioside It was confirmed that the solubility was significantly increased compared to. In addition, after dissolving resveratrol in a solution containing stevioside, it was confirmed that the amount of solubilized resveratrol was improved to 9,400 mg / L when the microwave treatment.

In addition, the amount of finally solubilized glucosyl resveratrol in the sample added with 20% (w / v) stevioside was 8,500 mg / L, which was added to 50 mg / L solubilized glucosyl resveratrol in a solution containing no stevioside. Solubility was significantly increased in comparison. In addition, when microwave treatment of the solution containing steviosides was confirmed that the amount of solubilized resveratrol improved to 14,200 mg / L, which was 71.5% solubilization of the initially added glucosyl resveratrol.

From the above results, when resveratrol or glucosyl resveratrol is dissolved in a solution containing steviosides, their solubility is significantly increased than when dissolved in a solution containing steviosides. After dissolving in the solution contained microwave treatment, it was found that their solubility is further increased.

In the same manner, 0, 10, 20% (w / v) licorice extract was added to dissolve the resveratrol at a concentration of 20 mg / ml, and treated at 70 ° C. for 15 minutes using ultrasonic waves. In addition, the amount of curcumin solubilized from each solvent by treating twice with 15 minutes using a microwave was quantified by HPLC, and the results are shown in Table 7 below.

TABLE 7

Licorice extract concentration,% (w / v)	Solubilized Resveratrol (mg / L)		Solubilized Glucosyl Resveratrol (mg / L)
	Ultrasonic treatment	Microwave	Ultrasonic treatment	Microwave
0	10	100	50	530
10	5,400	7,700	5,400	14,200
20	8,600	11,600	10,500	17,300

As shown in Table 7, the amount of resveratrol finally solubilized in the sample to which 20% (w / v) licorice extract was added was 8,600 mg / L, which was added to 10 mg / L solubilized resveratrol in a solution that did not contain licorice extract. It was confirmed that the solubility was significantly increased. In addition, after dissolving resveratrol in a solution containing licorice extract, when the microwave treatment, the amount of solubilized resveratrol was confirmed to improve to 11,600 mg / L.

In addition, the amount of finally solubilized glucosyl resveratrol in the sample to which 20% (w / v) licorice extract was added was 10,500 mg / L, which was added to 50 mg / L solubilized glucosyl resveratrol in a solution containing no licorice extract. Solubility was significantly increased in comparison. In addition, when microwave treatment of the solution containing licorice extract was confirmed that the amount of solubilized resveratrol improved to 17,300 mg / L, which was solubilized 86.5% of the initially added glucosyl resveratrol.

From the above results, when resveratrol or glucosyl resveratrol is dissolved in a solution containing licorice extract, their solubility is markedly increased than when dissolved in a solution that does not contain licorice extract. After dissolving in the solution contained microwave treatment, it was found that their solubility is further increased.

Example 6: Solubilization of Glycyrrhizinic Acid Using Steviol Glycosides and Licorice Extract

The glycyrrhizinic acid purchased from Sigma was dissolved in each 0.1-5% (w / v) stevioside or licorice extract solution at a concentration of 5 mg / ml and treated for 10 minutes using ultrasonic waves at 70 ° C. In addition, the amount of glycyrrhizinic acid solubilized from each solvent by treating twice with 15 minutes using microwave was quantified using HPLC.

It was confirmed that the gelation reaction occurred rapidly in the sample to which stevioside was added 0.1-0.5% (w / v). In addition, only 1-2% (w / v) stevioside addition succeeded in solubilizing all of the initially added glycyrrhizinic acid.

In addition, the result of using licorice extract in this manner also showed a gelation reaction when the licorice extract of 0.1-0.5% (w / v) was added, and all of the glycidyl acid initially added with only 1% or more licorice extract was solubilized. It was confirmed to make.

Example 7: Solubilization of Paclitaxol Using Steviol Glycosides and Licorice Extract

Paclitaxol purchased from Sigma was dissolved in each of 0, 5, 10, 20% (w / v) stevioside or licorice extract solution at a concentration of 10 mg / ml, and 10 minutes using ultrasonic waves at 70 ° C. The amount of curcumin solubilized from each solvent was treated and treated twice with 15 minutes using microwaves to quantify using HPLC.

When stevioside was added at a concentration of 10% and 20% (w / v) and sonicated, 300 mg / L and 700 mg / L were solubilized, but 1,400 mg / L and 2,100 when microwaved twice every 15 minutes. It could be seen that mg / L is solubilized.

For licorice extract, the solubilization was less than that of stevioside in 10% and 20% (w / v) solutions, but 800 mg / L and microwaves were sonicated when sonicated in 20% (w / v) solutions. It was found that solubilization was improved to 1,900 mg / L when treated.

Claims (27)

1. Steviol glycosides or stevia, or licorice comprising the same; And a poorly soluble material.
2. The complex of claim 1, wherein the stevia or licorice is in the form of an extract thereof.
3. The complex according to claim 1, wherein the complex is a formulation obtained by treating microwaves with a mixture of steviol glycosides, stevia, or licorice in a solvent, and a poorly soluble substance.
4. 4. The composite of claim 3 wherein said microwave treatment is reacted in a running microwave.
5. The composite according to claim 3, wherein the microwave treatment is performed for 1 to 20 minutes at 600 W to 800 W in a microwave oven.
6. The composite according to claim 3, wherein the microwave treatment is performed for 1 to 20 minutes at 140 W to 240 W in a microwave oven.
7. The method of claim 1, wherein the steviol glycosides are stevioside, enzymatically treated stevioside, rebaudioside, rubusoside, dulcoside, steviool bioside, or stevioside. The complex is a viol monoside.
8. The complex of claim 1, wherein the poorly soluble substance is a curcuminoid compound, resveratrol, glucosyl resveratrol, glycyrrhizinic acid, or paclitaxel.
9. The complex of claim 1, wherein the steviol glycoside is included at a concentration of 1 to 25% (w / v).
10. The complex of claim 1, wherein the licorice is included at a concentration of 0.1 to 25% (w / v).
11. A pharmaceutical or food composition comprising the complex of any one of claims 1 to 10.
12. Cosmetic composition comprising the complex of any one of claims 1 to 10
13. Feed composition comprising a complex of any one of claims 1 to 10.
14. A first step of mixing a steviol glycoside or stevia or licorice comprising the same, and a poorly soluble substance; And

    And a second step of subjecting the mixture obtained in the first step to microwave (microwave).
15. The method of claim 14, wherein the first step is mixing in a solvent or adding a poorly soluble substance to a solution containing steviol glycosides or stevia or licorice.
16. 15. The method of claim 14, wherein the second step is to treat the microwave for 1 to 20 minutes at 600 W to 800 W in a microwave oven.
17. 15. The method of claim 14, wherein the second step is to treat the microwave at 140 W to 240 W for 1 to 20 minutes in a microwave oven.
18. The method of claim 14, wherein the steviol glycoside is stevioside, enzymatically treated stevioside, rebaudioside, rubusoside, steviool bioside, or steviol monoside.
19. The method of claim 14, wherein the poorly soluble substance is a curcuminoid compound, resveratrol, glucosyl resveratrol, glycyrrhizinic acid, or paclitaxel.
20. The method of claim 14, wherein the steviol glycosides in the first step are included at a concentration of 1 to 25% (w / v).
21. The method of claim 14, wherein the licorice in the first step is characterized in that it comprises a concentration of 0.1 to 25% (w / v).
22. The method of claim 14, wherein the first step is performed at 60 ° C to 80 ° C.
23. 15. The method of claim 14, further comprising the step of sonicating the resultant of the first step prior to the second step.
24. Solubilizer containing licorice as an active ingredient.
25. The solubilizer of claim 24 wherein the licorice is in the form of a licorice extract.
26. A method of solubilizing poorly soluble material, comprising mixing licorice with poorly soluble material.
27. Use of licorice in the preparation of solubilizers.

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