WO2020209818A1

WO2020209818A1 - Probiotic wet wipes

Info

Publication number: WO2020209818A1
Application number: PCT/TR2020/050312
Authority: WO
Inventors: Servet GERZ
Original assignee: Aksan Kozmetik Sanayi Ve Ticaret Anonim Sirketi
Priority date: 2019-04-12
Filing date: 2020-04-13
Publication date: 2020-10-15
Also published as: TR201905516A2

Abstract

The present invention relates to novel probiotic wet wipes that are used in cosmetic and self-care sector and preferred for the purpose of its care function through cleaning and/or natural protection and natural moisturizing in daily life, wherein it discloses the wet wipes comprising a wetting solvent with Lactobacillus fermente at the rate of 0.00075% to 3% by weight, Lactobacillus at the rate of 0.0002% to 0.8% by weight, extract of Cocos Nucifera (Coconut) at the rate of 0.00005% to 0.2% by weight, ethylhexylglycerin at the rate of 0.001% to 1.5% by weight and purified water at the rate of 94.5% to 99.998% as a solvent and the preparation process thereof.

Description

PROBIOTIC WET WIPES

Technical Field

The present invention relates to wet wipes that are used in the cosmetic and self-care sector and preferred for the purpose of its care function through cleaning and/or natural protection and natural moisturizing in daily life, wherein it is disclosed novel wet wipes containing probiotic.

State of the Art

Use of wet wipes becomes more and more common in areas such as self-care and cleaning requirements in daily life, removing stinks resulting from causes such as sweating, providing practical cleaning in houses and workplaces and wiping off babies .

However, one of the most common problems pertaining to the wet wipes is that chemicals such as cleaning alcohol and disinfectants used in the wet wipes damage and dry the skin. Furthermore, perfumes and essences contained in the wet wipes to mask foul smells clog pores on the skin, thereby causing significant damage to the skin due to their chemical ingredients.

It is aimed at the state of the art that to develop wet wipes with natural components so as to overcome the above-mentioned problems. The invention subject to the registration titled "Carbonate wet wipes" numbered 2012/03199 relates to sodium bicarbonate wet wipes to prevent sweating and stinks on the human body and to avoid acne occurrences on the pimply face.

Another document is the invention subject to the registration titled "Special cleaning and skin-care wipes with olive oil for babies" numbered 2010/05675, which relates to a skin-care product comprising wipe (paper material), natural olive oil and hygienic chemical substance.

In order to overcome the above-mentioned problems, the inventor aims to develop novel probiotic wet wipes with natural content and productions method thereof.

Probiotics, as commonly known, are live microorganisms that are good for human health when taken in sufficient amounts. Probiotics, known as beneficial bacteria, that protect human health, are used to re-regulate intestinal flora impaired due to antibiotics use in the state of the art. The intestinal flora has a direct effect on skin health. That there is a sufficient number of probiotics in the intestine ensures a healthy skin structure. Impaired intestinal flora causes skin aging.

Description of the Invention

The object of the invention is to overcome the above-mentioned problems in the state of the art. Another object of the invention is to develop a product comprising natural components, protecting skin, providing a healthy skin structure, moisturizing skin by means of the inventive wet wipes with probiotics that are known to be beneficial bacteria.

It is suitable to use for babies and children due to its natural content. Furthermore, probiotic wet wipes also protect the skin against environmental factors, external factors and cracking due to hot-cold weather. It prevents skin aging by balancing the skin flora, provides skin brightness by moisturizing the skin and also cleans the skin.

The inventive probiotic wet wipes comprise Lactobacillus Fermente at the rate of 0.00075% to 3% by weight, Lactobacillus at the rate of 0.0002% to 0.8% by weight, extract of Cocos Nucifera (Coconut) at the rate of 0.00005% to 0.2% by weight, ethylhexylglycerin at the rate of 0.001% to 1.5% by weight and purified water at the rate of 94.5% to 99.998% as a solvent.

An important feature of the invention is that it does not comprise preservatives, alcohol, perfume, paraben, allergen, and colorants. Herein, the purpose is to use products with natural content .

Another object of the invention is to protect the natural flora of a person's skin and support the barrier function of the skin, including babies. When the inventive wet wipes are used, the lipid barrier is reinforced by means of the wetting solvent that it comprises and thus, the skin becomes more resistant to drying. Furthermore, it soothes skin sensitivity by means of regulating and strengthening the microbiome balance of the skin.

It provides a natural moisturizing, natural care and natural antimicrobial effect by means of its natural contents. The present invention aims to protect the vernix from chemicals. The vernix layer balances the body temperature of the baby/person and protects it from external factors. It is provided natural protection and care by means of probiotics.

Description of the Figures

FIGURE 1 illustrates the graphical representation of the increase in the moisturizing feature

FIGURE 2 illustrates the graphical representation of the comparative results of the moisturizing feature

Disclosure of the Reference Numbers No. Reference Name

T1 1. Week T2 2. Week

T3 3. Week

T4 4. Week

T24 24. Hours 1 lotion containing a mixture of 2% Lactobacillus fermente,

Lactobacillus, Cocos nucifera (coconut) extract

2 base lotion

3 unprocessed control

4 comparison between the lotion containing a mixture of 2% Lactobacillus fermente, Lactobacillus, Cocos nucifera (coconut) extract and the unprocessed control

5 comparison between the lotion containing a mixture of 2%

Lactobacillus fermente, Lactobacillus, Cocos nucifera (coconut) extract and the base lotion XI average impedance

X2 percentage (%) difference

Detailed Description of the Invention

Healthy skin flora is ensured by means of the multi-purpose activity of probiotics through the formulation of the inventive probiotic wet wipes with a wetting solvent comprising Lactobacillus fermente at the rate of 0.00075% to 3% by weight, Lactobacillus at the rate of 0.0002% to 0.8% by weight, extract of Cocos Nucifera (Coconut) at the rate of 0.00005% to 0.2% by weight, ethylhexylglycerin at the rate of 0.001% to 1.5% by weight and purified water at the rate of 94.5% to 99.998% as a solvent . In an embodiment of the invention, the wetting solvent comprises a mixture of 2% (in total) Lactobacillus fermente, Lactobacillus and Cocos Nucifera (coconut) extract.

In the wetting solvent that the wet wipes comprise, Lactobacillus and Lactobacillus fermente are used as probiotics. The Lactobacillus fermente provides efficiently the antibacterial effect of Lactobacillus and the antifungal effect of Cocos Nucifera (coconut) . The Lactobacillus fermente is a probiotic- based component, that is formed through the fermentation of Lactobacillus in a certain growth medium and certain conditions. Lactobacillus is one of the microorganisms that are used in obtaining the fermented products being kimchi of pickled cabbage and a Korean diet fiber. Lactobacillus may limit the growth of other microorganisms by acidifying its medium just as most members of the lactic acid bacteria family. Furthermore, Lactobacillus also produces new antimicrobial peptides that provide protection against bacteria.

The product gains cellular viability increasing feature and skin moisturizing feature by means of Cocos Nucifera (Coconut) extract in the wetting solvent.

Ethylhexylglycerin, being another component, is used as the wetting material in the invention. It is introduced to increase the moisturizing effect of the formulation and to soften the skin. Another function thereof is to be protective.

Modern fermentation and bioprocess technology are utilized in the preparation of the inventive probiotic wet wipes with natural components. Natural probiotics, purified water and a product with organic components are obtained by fermenting Cocos Nucifera (Coconut) with Lactobacillus.

Firstly, the Lactobacillus fermente, Lactobacillus, and Cocos Nucifera (coconut) extract are mixed in the preparation of the wetting solvent of the inventive probiotic wet wipes. Then, the purified water is taken into the container and afterward, the mixture of the Lactobacillus fermente, Lactobacillus, Cocos Nucifera (Coconut) extract is introduced. Then, it is mixed and ethylhexylglycerin is added after mixing and the mixing process is performed again. The wipe is immersed into the wetting solvent and the solved is absorbed and then, the prepared wet wipes are packaged .

A sample formulation for the wetting solvent of the inventive probiotic wet wipes is given in Table 1. Furthermore, the formulation on Table 1 comprises the Lactobacillus fermente, Lactobacillus and Cocos Nucifera extract such that its rate is 2% by weight, constituting an example for an embodiment of the invention .

Table 1: Sample formulation of the wetting solvent

The inventor conducted an in-vivo study in a period of four weeks to evaluate the benefits of moisturizing the inventive probiotic wet wipes. 10 people were enrolled in the study between the ages of 23-45. The moisturizing experiment was conducted to evaluate the moisturizing capability of the product, wherein the results thereof are given graphics of Figure 1 and Figure 2.

10 volunteers between the ages of 23-45 without any known skin pathology were enrolled. A DermaLab Corneometer was used to measure moisture levels of the volar forearm of subjects. Corneometer is a device to measure the water amount on the skin. Therefore, the higher the moisture level is, the higher the reading through the Corneometer will be. On the first day of the study, basal moisturizing readings were taken. After the first measurements, it was asked from all subjects to apply 2mg of each test material on their volar forearms. Measurements were taken immediately after the application of test materials and once a week throughout four weeks. The test material is comprised of a mixture of 2% Lactobacillus fermente, Lactobacillus, Cocos nucifera (coconut) extract in the base lotion. In Figure 1, the lotion containing a mixture of 2% Lactobacillus fermente, Lactobacillus, Cocos nucifera (coconut) extract, the base lotion, and the unprocessed control samples were compared in terms of the moisturizing increase depending on time. In the graph, average impedance values in 24 hours (T24), in the first week (Tl), in the second week (T2), in the third week (T3), in the fourth week (T4) are respectively illustrated through columns in terms of the lotion containing a mixture of 2% Lactobacillus fermente, Lactobacillus, Cocos nucifera (coconut) extract, the base lotion, and the unprocessed control samples. Result values are given in Table 2 and Table 3 in detail.

Table 2

Table 3

The results for the comparative moisturizing in the study are given in Figure 2. Referring to Figure 2, the moisture levels were increased by 42.42% after 24 hours (T24) and by 63% at the fourth week (T4) compared to the unprocessed control as evidence of the four- weeks efficiency study of the inventive lotion containing a mixture of 2% Lactobacillus fermente, Lactobacillus, Cocos nucifera (coconut) extract. When compared to the base lotion, it was observed to increase the moisturizing by 14,38% after 24 hours (T24) and by 25% after four weeks (T4) . The results exhibit that the inventive product is capable of increasing the moisturizing when compared to both the unprocessed control and the base lotion. This study confirms that the product may ensure a significant moisturizing and benefits in skin hydration when added to the cosmetic applications. Depending on the results, adding this innovative product ensures the formulator to benefit from both the natural antimicrobial features of the product and the capability of introducing a significant moisturizing effect into the cosmetic formulation. Furthermore, these features are ideal for applications on various skin and scalp conditions. The results exhibit that the product is capable of increasing significantly the moisturizing compared to the control.

Claims

1. Probiotic wet wipes, characterized in that the wetting solvent thereof comprises Lactobacillus fermente at the rate of 0.00075% to 3% by weight, Lactobacillus at the rate of 0.0002% to 0.8% by weight, Cocos Nucifera (Coconut) extract at the rate of 0.00005% to 0.2% by weight as probiotics .

2. Probiotic wet wipes according to Claim 1, characterized in that the wetting solvent thereof comprises in total 2% Lactobacillus fermente, Lactobacillus and Cocos Nucifera

(coconut) extract by weight.

3. Probiotic wet wipes according to Claim 1 or 2, characterized in that the wetting solvent thereof comprises ethylhexylglycerin at the rate of 0.001% to 1.5% by weight.

4. Probiotic wet wipes according to Claim 3, characterized in that the wetting solvent thereof comprises purified water at the rate of 94.5% to 99.998% by weight.

5. The preparation process of probiotic wet wipes according to Claim 4, characterized in that it comprises the process steps of; mixing Lactobacillus fermente, Lactobacillus, Cocos Nucifera (coconut) extract, introducing the purified water into the container, adding the mixture of Lactobacillus fermente, Lactobacillus, Cocos Nucifera (coconut) extract into the container, stirring the mixture, adding ethylhexylglycerin, stirring thereof, having the solvent absorbed by immersing wipes into the prepared wetting solvent.