WO2020209609A2 - Dispositif d'échange de tamis, système de réduction de taille de tissu biologique le comprenant, procédé de réduction de taille de tissu biologique l'utilisant, et procédé de séparation d'une substance cible à partir d'un tissu biologique pertinent - Google Patents

Dispositif d'échange de tamis, système de réduction de taille de tissu biologique le comprenant, procédé de réduction de taille de tissu biologique l'utilisant, et procédé de séparation d'une substance cible à partir d'un tissu biologique pertinent Download PDF

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Publication number
WO2020209609A2
WO2020209609A2 PCT/KR2020/004773 KR2020004773W WO2020209609A2 WO 2020209609 A2 WO2020209609 A2 WO 2020209609A2 KR 2020004773 W KR2020004773 W KR 2020004773W WO 2020209609 A2 WO2020209609 A2 WO 2020209609A2
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WO
WIPO (PCT)
Prior art keywords
screen
biological tissue
cover
opening
hole
Prior art date
Application number
PCT/KR2020/004773
Other languages
English (en)
Korean (ko)
Other versions
WO2020209609A3 (fr
Inventor
이준석
콥쿠이레이
Original Assignee
이준석
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020190131097A external-priority patent/KR102202861B1/ko
Application filed by 이준석 filed Critical 이준석
Priority to EP20788402.4A priority Critical patent/EP3954405B1/fr
Priority to EP23206763.7A priority patent/EP4289925A3/fr
Priority to CN202080005584.5A priority patent/CN112867571B/zh
Priority to JP2021544086A priority patent/JP7201830B2/ja
Priority to US17/281,999 priority patent/US20210353837A1/en
Publication of WO2020209609A2 publication Critical patent/WO2020209609A2/fr
Publication of WO2020209609A3 publication Critical patent/WO2020209609A3/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/02—Blood transfusion apparatus

Definitions

  • embodiments relate to a screen exchange device, a system for refining a biological tissue including the same, a method for refining a biological tissue using the same, and a method for separating a target material from a biological tissue related thereto.
  • adipose tissue is generally used to decompose and centrifuge the tissue using enzymes, but there is no appropriate medical enzyme, and the enzyme used is toxic, so the safety of substances obtained from adipose tissue degraded using enzymes is not affected. There is a controversy about it. Thus, there are various attempts to obtain substances that can be used for regenerative treatment and cosmetic purposes from adipose tissue without using enzymes.
  • US Patent Publication No. 5,480,553 discloses a hollow filter membrane module.
  • An object according to an embodiment is to provide a screen exchange device that minimizes disassembly and coupling with a container holding a living tissue, a system for refining a living body including the same, and a method for refining a living body using the same.
  • An object according to an embodiment is to provide a method for effectively separating various target materials having various sizes and specific gravity from living tissue.
  • an apparatus for exchanging a screen for refining a biological tissue may include a disk including a plurality of screens having at least one through-hole for minimizing a living body and having different through-hole characteristics; A first cover covering a first side of the disk and including a first opening through which a living tissue passes; A second cover covering a second side of the disk and including a second opening through which the living tissue passes; A housing accommodating the disk; And a manipulator that selects a screen among the plurality of screens and manipulates the first cover or the second cover so that the selected screen communicates with the first opening and the second opening.
  • the manipulator includes a first handle configured to rotate the first cover relative to the disk and a second handle configured to rotate the second cover relative to the disk.
  • the first handle is positioned at any one position between a locking position in which rotation of the first cover with respect to the disk is fixed and an operating position in which rotation of the first cover with respect to the disk is allowed. Any one of a locking position in which rotation of the second cover with respect to the disk is fixed, and an operating position in which rotation of the second cover with respect to the disk is allowed, and the second cover is operably coupled to the first cover.
  • the second handle is operatively coupled to the second cover to be in position.
  • the first handle and the second handle include a central portion; A radially extending portion extending from the central portion; And a circumferential direction extending portion extending in a direction crossing the extending direction of the radial direction extending portion.
  • the circumferential extension includes an engagement element configured to engage the inner surface of the housing.
  • the through-hole characteristics are selected from the group including the shape of the through-hole, the width of the through-hole, the depth of the through-hole, the position of the through-hole formed on the screen, and the arrangement of the plurality of through-holes when there are multiple through-holes. It includes at least one or more.
  • the plurality of screens each include a protrusion formed at an angle with respect to the screen from an edge defining the through hole, protruding toward the first cover or the second cover, and configured to scratch and tear the living tissue passing through the through hole. do.
  • the biological tissue refining apparatus further includes a first spacer disposed between the first cover and the disk, and a second spacer disposed between the second cover and the disk.
  • the first cover further includes a third opening through which the living tissue passes, and the second cover further includes a fourth opening through which the living tissue passes.
  • the at least one through-hole has an edge, and the edge contacts the living body tissue passing through the through hole and presses the living body tissue, thereby scratching and tearing the living body tissue, thereby reducing the living body tissue.
  • a system for refining a biological tissue includes the screen exchange device; A first syringe including a first container configured to engage the first opening and to receive a living tissue, and a first push rod configured to apply pressure to the first container; And a second syringe including a second container configured to engage with the second opening and accommodate a living tissue, and a second push rod configured to apply pressure to the second container, wherein the first push rod is the When pressure is applied to the first container, the biological tissue of the first container passes through the first opening, the selected screen, and the second opening in order to move to the second container, and the second push rod is When pressure is applied to the second container, the biological tissue of the second container passes through the second opening, the selected screen, and the first opening in sequence, and may take a form between a second shape that moves to the first container. .
  • a method for refining a living body tissue comprises: coupling a first syringe and a second syringe including at least a living tissue to the first opening and the second opening of the screen exchange device, respectively; Selecting a first screen from among the plurality of screens using the manipulator; Pressing the first syringe to move the biological tissue to the second syringe through the first opening, the first screen, and the second opening, and press the second syringe to the second opening, the first screen And moving the biological tissue to the first syringe through the first opening, and first miniaturizing the biological tissue.
  • the remaining plurality of screens having through-hole characteristics having different through-hole characteristics of the first screen and the through-hole characteristics of the second screen among the plurality of screens are sequentially selected to refine the biological tissue. It may further include the step of.
  • a method of separating a target material from a biological tissue is a first centrifugation step of centrifuging the biological tissue at a first centrifugal acceleration to remove a non-target material and obtain a plurality of types of materials including the target material. ; Micronizing the remaining biological tissues by sequentially selecting a plurality of screens having different through-hole characteristics; A second centrifugation step of centrifuging at a second centrifugal acceleration different from the first centrifugal acceleration with respect to the obtained plurality of materials including the remaining biological tissues; And a first separation step of separating the material layer containing the target material.
  • the step of micronizing the remaining biological tissue may be repeatedly performed a plurality of times.
  • the method may further include removing air mixed with the micronized biological tissue after the micronizing of the biological tissue.
  • the method may further include the step of separating the biological tissue from the material containing the target material by compressing the micronized biological tissue after the step of micronizing the biological tissue.
  • the method may further include mixing the set solution in the separated material layer and then miniaturizing the materials included in the mixed solution using a screen having a set through-hole characteristic.
  • the method includes a third centrifugation step of performing centrifugation on the mixed solution at a third centrifugal acceleration different from the first centrifugal acceleration and the second centrifugal acceleration; And a second separation step of separating the material layer containing the target material.
  • the method may further include removing air mixed in the mixture of the separated material layer and the set solution.
  • the method includes a third centrifugation step of performing centrifugation on the mixed solution at a third centrifugal acceleration greater than the first centrifugal acceleration and equal to or greater than the second centrifugal acceleration, and separating the material layer containing the target material. It may further include a second separation step.
  • a biological tissue refining system including the same, and a biological tissue refining method using the same can minimize disassembly and coupling with a container holding a biological tissue.
  • the method according to an embodiment can effectively separate various target materials having various sizes from biological tissues.
  • a biological tissue refining system including the same, a biological tissue refining method using the same, and a method of separating a target material from a biological tissue related thereto are not limited to those mentioned above, and are not mentioned. Other effects that are not will be clearly understood by those skilled in the art from the following description.
  • FIG. 1 is a perspective view of a screen changing device according to an embodiment as viewed from one direction.
  • FIG. 2 is a perspective view of a screen changing device according to an exemplary embodiment viewed from another direction.
  • FIG 3 is an exploded perspective view of a screen exchange device according to an embodiment.
  • FIG. 4 is an exploded perspective view of some components of the screen changing device according to an embodiment.
  • FIG. 5 is a cutaway perspective view of a partial structure of a screen exchange device according to an embodiment.
  • FIG. 6 is a plan view of a system for refining a biological tissue including a screen exchange device according to an exemplary embodiment.
  • FIG. 7 is a cross-sectional perspective view of the screen exchange device of FIG. 6 taken along line B-B.
  • FIG. 8 is a cross-sectional front view of the screen changing device of FIG. 6 taken along line B-B.
  • FIG. 9 is a view showing a screen according to an embodiment.
  • FIG. 10 is a diagram illustrating an example of a partial structure of a screen changing device according to an embodiment.
  • FIG. 11 is a view showing another example of a partial structure of the screen changing device according to an embodiment.
  • first, second, A, B, (a), (b) may be used. These terms are only used to distinguish the component from other components, and the nature, order, or order of the component is not limited by the term.
  • a component is described as being “connected”, “coupled” or “connected” to another component, that component may be directly connected or connected to that other component, but another component between each component It should be understood that may be “connected”, “coupled” or “connected”.
  • the screen exchange device 10 includes a plurality of screens 122a, 122b, 122c, 122d, and 122e each including a plurality of through-holes configured to refine a biological tissue.
  • the second screen 122b is selected among the plurality of screens 122a, 122b, 122c, 122d, and 122e,
  • the selected second screen 122b may be configured to refine a biological tissue.
  • the biological tissue may be an adipose tissue, but is not limited thereto.
  • the screen exchange device 10 includes a housing 110, a disk 120, a first sealing part 130, a second sealing part 140, a first cover 150, a second cover 160, and a manipulator 170. , 180) and a support part 190 may be included.
  • the housing 110 may be configured to accommodate the disk 120, the first sealing portion 130, the second sealing portion 140, the first cover 150 and the second cover 160.
  • the housing 110 may be configured to seal the disk 120 to the outside together with the first sealing portion 130 and the second sealing portion 140.
  • the housing 110 may have a substantially cylindrical shape.
  • the housing 110 may have an open first side and an open second side opposite to the first side.
  • the housing 110 may include a first mount 111, a second mount 112, a third mount 113, a first slot 114 and a second slot 115.
  • the disk 120 may be mounted on the first mount 111.
  • the disk 120 may be fixed to the first mount 111.
  • the disk 120 may be configured to rotate with the housing 110.
  • the first mount 111 may be formed on the inner surface of the middle portion of the housing 110.
  • a first sealing part 130 and a first cover 150 may be mounted on the second mount 112.
  • the second mount 112 may be formed on the inner surface of the first side based on the middle portion of the housing 110.
  • the second sealing part 140 and the second cover 160 may be mounted on the third mount 113.
  • the third mount 113 may be formed on an inner surface of the second side opposite to the first side based on the middle portion of the housing 110.
  • the first circumferential extension 173 of the first handle 170 may be mounted on the first slot 114.
  • the first circumferential extension 173 of the first handle 170 may be releasably engaged with the first slot 114.
  • the first slot 114 may allow movement of the first circumferential extension part 173 in the circumferential direction.
  • the first slot 114 may be formed on the inner surface of the first side of the housing 110 adjacent to the second mount 112.
  • the second circumferential extension 183 of the second handle 180 may be mounted on the second slot 115.
  • the second circumferential extension 183 of the second handle 180 may be releasably engaged with the second slot 115.
  • the second slot 115 may allow the movement of the second circumferential extension part 183.
  • the second slot 115 may be formed on the inner surface of the second side of the housing 110 adjacent to the third mount 113.
  • the disk 120 may include a plate 121 and a plurality of screens 122a, 122b, 122c, 122d, and 122e.
  • the plate 121 may be fixed to the first mount 111.
  • the plate 121 may include a first central opening 1211.
  • the plate 121 may have a substantially circular cross section.
  • the plurality of screens 122a, 122b, 122c, 122d, and 122e may be spaced apart from each other in the circumferential direction of the plate 121 around the first central opening 1211 and may be installed on the plate 121.
  • the plurality of screens 122a, 122b, 122c, 122d, and 122e may include at least one through hole configured to micronize a biological tissue.
  • the plurality of screens 122a, 122b, 122c, 122d, and 122e may have different through-hole characteristics.
  • the through-hole characteristics include the shape of the through-hole, the size of the through-hole, the depth of the through-hole, the position of the through-hole formed on the screen, the angle formed by some sections of the through-hole with the plate 121, and the number of through-holes. In some cases, it may include an arrangement of a plurality of through holes.
  • the plurality of screens (122a, 122b, 122c, 122d, 122e) have different through-hole characteristics, for example, each through-hole of the plurality of screens (122a, 122b, 122c, 122d, 122e) It could mean that the sizes of are different.
  • the size of the through hole refers to the maximum length of the through hole that crosses the through hole.
  • the through hole may mean the diameter of the through hole.
  • the shape of the through hole may be a circular shape, a polygonal shape, or a shape composed of a plurality of edges.
  • the first sealing part 130 may perform sealing between the disk 120 and the first cover 150.
  • the first sealing part 130 is disposed between the disk 120 and the first cover 150, and may be installed on the inner surface of the housing 110 along the circumferential direction of the housing 110.
  • the first sealing part 130 may have a ring shape.
  • the second sealing part 140 may perform sealing between the disk 120 and the second cover 160.
  • the second sealing part 140 may be disposed between the disk 120 and the second cover 160 and installed on the inner surface of the housing 110 along the circumferential direction of the housing 110.
  • the second sealing part 140 may have a ring shape.
  • the first cover 150 is configured to cover the first side of the disk 120.
  • the first cover 150 may include a first base 151, a first connection part 152 having a first opening, and a first fastening part 154.
  • the first base 151 may have a substantially circular cross section.
  • the first opening of the first connecting portion 152 is configured to allow the living tissue to pass through.
  • the first opening may penetrate the first base 151.
  • the first connection part 152 may be configured to connect to a container containing a living tissue or a hollow container.
  • the first connection part 152 may be configured to protrude from the first base 151.
  • the first connection part 152 may be formed at the periphery of the first base 151.
  • the first handle 170 may be coupled to the first fastening part 154.
  • the first fastening part 154 is a first guide configured to guide the movement of the first fastening hole 1541 and the first handle 170 in the axis (X) direction through which the first locker 191 of the support part 190 passes. (1542) may be included.
  • the first fastening part 154 may be formed in the center of the first base 151.
  • the first cover 150 may further include a third connection part 153 having a third opening.
  • the third opening of the third connection part 153 is configured to allow the living tissue to pass through.
  • the third connection part 153 may be configured to connect to a container containing a biological tissue or an empty container.
  • the third connection part 153 may be configured to protrude from the first base 151.
  • the third connection part 153 may be formed on the first base 151 to be spaced apart from the first connection part 152.
  • the third connection part 153 may be formed at the periphery of the first base 151.
  • the third opening of the third connection part 153 may be configured to communicate with a screen having the largest through hole among the plurality of screens 122a, 122b, 122c, 122d, and 122e.
  • the user connects the container containing the lump-shaped biological tissue to the third connection part 153 to pass the biological tissue through the third opening, and roughly the biological tissue with a screen having the largest through hole ( roughly) after miniaturization, connect the container to the first connection part 152 again, select a screen with desired through-hole characteristics among the plurality of screens 122a, 122b, 122c, 122d, 122e, and use the selected screen
  • the second cover 160 is configured to cover a second side opposite to the first side of the disk 120.
  • the second cover 160 may include a second base 161, a second connecting portion 162 having a second opening, and a second fastening portion 164.
  • the second fastening part 164 may include a second fastening hole and a second guide (not shown).
  • the second cover 160 may further include a fourth connection part 163 having a fourth opening. Structures, functions, and effects of the second base 161, the second connection part 162, the fourth connection part 163, and the second fastening part 164 of the second cover 160 are previously described unless otherwise described herein. It can be understood that the structure, function and effect of the first base 151, the first connection part 152, the third connection part 153, and the first fastening part 154 of the described first cover 150 are substantially the same. have.
  • the manipulators 170 and 180 select at least one of a plurality of screens 122a, 122b, 122c, 122d, and 122e, and the selected screen is the first opening of the first connection part 152 and the second connection part (
  • the first cover 150 and the second cover 160 may be operated to communicate with the second opening of 162 or the third opening of the third connection 153 and the fourth opening of the fourth connection 163.
  • the manipulators 170 and 180 may include a first handle 170 and a second handle 180. However, although the manipulators 170 and 180 are described as including the first handle 170 and the second handle 180, only one of the first handle 170 and the second handle 180 may be included. , It is also possible to perform the functions of the manipulators 170 and 180 in other possible mechanical methods, electronic methods, etc. not described herein.
  • the first handle 170 may manipulate the first cover 150 so that the first cover 150 rotates relative to the disk 120.
  • the first handle 170 may include a first central portion 171, a first radial extension 172, and a first circumferential extension 173.
  • the first central part 171 may be coupled to the first fastening part 154.
  • the first central part 171 may move along the first guide 1542 of the first fastening part 154.
  • the first central part 171 may include a second central opening 1711 through which the first locker 191 of the support part 190 passes.
  • the second central opening 1711 may be aligned with the first fastening hole 1541 of the first fastening part 154.
  • the first radial extension part 172 may extend in a radial direction of the first cover 150 from the first central part 171.
  • the first circumferential extension part 173 may extend in a direction crossing the extension direction of the first radiation direction extension part 172.
  • the first circumferential extension part 173 may have an arc shape centered
  • the first circumferential extension 173 may be configured to move along the inner surface of the housing 110 and releasably engage with the inner surface of the housing 110.
  • the first circumferential extension 173 may include a first moving element 1731 and a first engaging element 1732.
  • the first moving element 1731 may be configured to move in a circumferential direction along the first slot 114 of the housing 110.
  • the first engagement element 1732 may be configured to engage the first recess 1121 of the second mount 112 of the housing 110.
  • the first engagement element 1732 is a part of the second mount 112 of the housing 110. It may be engaged with the first recess 1121.
  • the first engagement element 1732 is the first of the second mount 112. Since it is engaged with the one recess 1121, the rotation of the first cover 150 with respect to the housing 110 and the disk 120 fixed to the housing 110 is fixed.
  • the first engagement element 1732 is the second mount 112 of the housing 110 ) May be released from the first recess 1121.
  • the first engaging element 1732 is Since it is released from the first recess 1121 of the mount 112, rotation of the first cover 150 with respect to the housing 110 and the disk 120 fixed to the housing 110 is allowed.
  • the user manipulates the first handle 170 to rotate the first cover 150 about the axis X, while rotating the first opening and the second connecting portion 153 of the first connecting portion 152 into a plurality of screens 122a, 122b, 122c, 122d, 122e) can be arranged on the desired screen.
  • the second handle 180 may manipulate the second cover 160 so that the second cover 160 rotates relative to the disk 120.
  • the second handle 180 may include a second central portion 181, a second radial extension 182, and a second circumferential extension 183.
  • the second circumferential extension 183 has a second movable element 1831 moving along the inner surface of the second slot 115 of the housing 110 and a second movable element 1831 of the third mount 113 of the housing 110. It may include a second engagement element 1832 configured to engage the recess 1131. Structures, functions and effects of the second central portion 181, the second radial extension portion 182, and the second circumferential extension portion 183 of the second handle 180 are described above unless otherwise described herein. It can be understood that the structure, function, and effect of the first central portion 171, the first radial extension 172, and the first circumferential extension 173 of the handle 170 are substantially the same.
  • the first handle 170 and the second handle 180 may be operated in conjunction with each other.
  • the first handle 170 and the second handle 180 are interlocked with each other. Since the first handle 170 and the second handle 180 are directly or indirectly connected to each other, the first handle 170 ) And the second handle 180 means that the other handle works together.
  • the first handle 170 and the second handle 180 are connected to each other through the first locker 191 of the support unit 190, the user can move the first handle 170 to the axis X.
  • the second handle 180 may rotate together with the first handle 170 while the housing 110 and the disk 120 are fixed.
  • the first handle 170 and the second handle 180 may be operated independently of each other.
  • the fact that the first handle 170 and the second handle 180 are independent of each other means that the first handle 170 and the second handle 180 are not connected to each other. 2
  • the first handle 170 and the second handle 180 may be configured not to be constrained to each other by the first locker 191 of the support unit 190.
  • the user rotates the first handle 170 by a first angle with respect to the axis X
  • the user rotates the second handle 180 by a second angle different from the first angle with respect to the axis X. I can make it.
  • the rotation directions of the first handle 170 and the second handle 180 with respect to the axis X may be the same or opposite to each other.
  • the support unit 190 is configured to support the first handle 170, the first cover 150, the second cover 160 and the second handle 180.
  • the support part 190 may include a first locker 191 and a second locker 192.
  • the first locker 191 passes through the second central opening 1711, the first fastening hole 1541, the first central opening 1211, the second fastening hole 1641, and the third central opening 1811. It can be combined with two lockers 192. Accordingly, the first handle 170, the first cover 150, the second cover 160, and the second handle 180 are axially aligned with respect to the housing 110 and the disk 120 (fixed) without eccentricity. It can rotate around (X).
  • the system 1 for refining a biological tissue may include a screen exchange device 10, a first syringe C1 and a second syringe C2.
  • the first syringe C1 is formed at the front end of the first container C11 and the first container C11 configured to accommodate the biological tissue BT, and the first connection part 152 or the third of the first cover 150
  • the first push rod (C13) configured to apply pressure to the inside of the first coupling portion (C12) and the first container (C11) configured to be coupled to the connection portion (153) and press the biological tissue (BT) toward the disk 120 ) Can be included.
  • the second syringe (C2) is formed at the tip of the second container (C21) and the second container (C21) configured to accommodate the biological tissue (BT), and the second connection part 162 or the fourth of the second cover 160 Pressure is applied to the inside of the second coupling part C22 and the second container C21 configured to be coupled to the connection part 163 (see FIGS. 2 and 3), and the biological tissue BT is pressed toward the disk 120 It may include a second push rod (C23) configured to be.
  • the user puts the biological tissue BT to be refined into the first container C11, and couples the first coupling part C12 to the first connection part 152. Thereafter, the user couples the second coupling part C22 of the second container C21 with no contents to the second connection part 162. Thereafter, the user manipulates the first handle 170 in the axial direction so that the first cover 150 is in the operating position. Thereafter, the user rotates the first cover 150 by rotating the first handle 170 in the axial direction, and the screen having the desired through-hole characteristics is provided with the first opening and the second connection part ( A desired screen is selected from among a plurality of screens to communicate with the second opening of 162).
  • the user manipulates the second handle 180 in the axial direction so that the second cover 160 is in the operating position. Thereafter, the user rotates the second handle 180 with respect to the axial direction to rotate the second cover 160, and the screen having the desired through-hole characteristics is provided with the second opening and the first connection part of the second connection part 162 ( The same screen as the previously selected screen is selected to communicate with the first opening of 152).
  • the biological tissue BT is refined while passing through the selected screen of the disk 120, and the second push rod C23 is retracted while moving to the second container C21.
  • the biological tissue BT is refined while passing through the selected screen, and the first push rod C13 is retracted while moving to the first container C11.
  • the user rotates the first handle 170 and the second handle 180 to select another screen having another through-hole characteristic, and the same process as above.
  • the biological tissue (BT) can be micronized.
  • the user may remove the first coupling portion C12. It can also be coupled to the three connection portion 153.
  • the third opening of the third connection part 153 may be aligned with the screen having at least one through hole having the largest width.
  • the user couples the second coupling portion C22 of the second container C21 to the fourth connection portion 163 (see FIGS. 2 and 3 ).
  • the fourth connector 163 may be aligned with the previously selected screen.
  • the user may roughly refine the biological tissue BT by using the first push rod C13 and the second push rod C23 in the same manner as described above.
  • the user separates the first container (C11) and the second container (C21) and reconnects the first coupling part (C12) and the second coupling part (C22) to the first connection part 152 and the second connection part 162, respectively. ), and the micronization process described above can be repeated.
  • FIG. 9 is a view showing a screen according to an embodiment.
  • the screen 220 may include a plate 221 and a through hole 222.
  • the through hole 222 may be defined by a plurality of corners of the plate 221. These edges are in contact with the living tissue passing through the through hole 222 and pressurize the living tissue, thereby scratching and tearing the living tissue, thereby reducing the size of the living tissue.
  • the screen 220 is formed in the first linear portion 223a formed on the first side of the through hole 222, the first side of the through hole 222, and is formed toward the center of the through hole 222.
  • the first protrusion 224a protruding in the one direction T1, the second linear part 223b formed on the second side of the through hole 222, and the through hole 222 are formed on the second side of the through hole 222 ) May include a second protrusion 224b protruding in the second direction T2 toward the center of the), so that the section of the edge in contact with the tissues passing through the through hole may be increased.
  • the first protrusion 224a and the second protrusion 224b may be oriented so as to be angled with respect to the screen 220 and may be configured to scratch and tear the biological tissue passing through the screen 220.
  • the first extension line L1 of the first protrusion 224a protruding in the first direction T1 and the second extension line L2 of the second protrusion 224b protruding in the second direction T2 are in a twisted position. There may be. In other words, the first extension line L1 and the second extension line L2 may neither be parallel to each other nor meet each other.
  • the first protrusion 224a and the second protrusion 224b are in the direction T1 of the first protrusion 224a and the second protrusion toward one of the first cover 150 and the second cover 160.
  • a direction T2 of 224b may be set.
  • FIG. 10 is a cross-sectional view showing an example of a partial structure of a screen exchange device according to an embodiment.
  • the screen exchange device 30 includes a first spacer 355 and a disk 120 and a second cover 160 installed between the disk 120 and the first cover 150. ) It may include a second spacer 365 installed between.
  • the first spacer 355 is configured to secure a first space S1 between the disk 120 and the first cover 150
  • the second spacer 365 is the disk 120 and the second cover 160 It may be configured to secure a second space (S2) therebetween.
  • the first spacer 355 and the second spacer 365 may be formed of any material suitable for securing the first space S1 and the second space S2.
  • the first spacer 355 and the second spacer 365 may include a metallic material.
  • the sizes of the first space S1 and the second space S2 may be maintained substantially the same during the operation of the screen exchange device 30.
  • the first spacer 355 and the second spacer 365 may include an elastic material or the like. In this case, the sizes of the first space S1 and the second space S2 may vary during the operation of the screen exchange device 30.
  • the first spacer 355 and the second spacer 365 may have any suitable shape so as not to interfere with the miniaturization of the biological tissue and rotation of the covers 150 and 160.
  • the first spacer 355 and the second spacer 365 may have an annular shape.
  • the first spacer 355 and the second spacer 365 may be disposed outside the plurality of screens 122 arranged in the circumferential direction around the first central opening 1211 of the plate 121.
  • FIG. 11 is a cross-sectional view illustrating still another example of a structure of a screen exchange device according to an embodiment.
  • the screen exchange device 40 includes a first spacer 455 and a disk 120 and a second cover 160 installed between the disk 120 and the first cover 150. ) May include a second spacer 465 installed between.
  • the difference from the embodiment of FIG. 10 is that the first spacer 455 and the second spacer 465 of the embodiment of FIG. 11 are arranged along the circumferential direction around the first central opening 1211 of the plate 121. It is disposed inside the plurality of screens 122.
  • a method of separating a tissue, a cell, and a target material by micronizing a biological tissue and removing a non-target material will be described.
  • the non-target material is interpreted as an unnecessary material.
  • the following examples relate to separating stromal vascular fraction (SVF) from adipose tissue, but it should be noted in advance that the method of separating biological tissue is not necessarily limited to the following examples.
  • Step 2 After centrifugation, material layers such as blood urea and sap are removed.
  • Step 3 the size of the adipose tissue is reduced by scraping and tearing the adipose tissue using the screen exchange device described above, and the combination of the tissue, cells, and substances constituting the adipose tissue is separated.
  • Step 4 Afterwards, centrifugation at high speed (RCF 500g ⁇ 2000g) is performed on the adipose tissue and the tissues, cells, and materials separated from the adipose tissue again to separate them once again.
  • RCF 500g ⁇ 2000g centrifugation at high speed
  • Step 5 After centrifugation, the material layer containing the stromal vascular fibrosis (SVF) collected farther from the center of rotation than the adipose tissue layer is separated.
  • SVF stromal vascular fibrosis
  • Step 6 After performing Step 3, a process of removing air mixed with fat tissue and substances can be added.
  • a screen having a through hole of 300 ⁇ m to 4000 ⁇ m may be selected from among a plurality of screens of the screen exchange device to scratch and tear adipose tissue.
  • Step 8 the first step of minimizing adipose tissue by selecting a screen having a through hole of 3000 ⁇ m to 4000 ⁇ m, and then selecting a screen having a through hole of 2000 ⁇ m to 3000 ⁇ m to refine the adipose tissue
  • a third step of selecting a screen having a through hole of 1000 ⁇ m to 2000 ⁇ m to refine the adipose tissue and then selecting a screen having a through hole of 500 ⁇ m to 1000 ⁇ m to refine adipose tissue.
  • Four steps can be performed sequentially.
  • Step 9 After the fourth step in step 8, a fifth step of miniaturizing adipose tissue by selecting a screen having a through hole of 300 ⁇ m to 500 ⁇ m may be added.
  • Step 10 By moving the fat tissue back and forth through the through hole of the screen 5 to 50 times in each step of Step 8 and Step 9, it is possible to refine the living body tissue.
  • the separated materials may be filtered through a screen or filter having a through hole of 50 ⁇ m to 200 ⁇ m.
  • Step 12 After performing step 5, after mixing saline or distilled water with the separated material layer, a screen having through holes of 500 ⁇ m to 1000 ⁇ m may be selected to refine the mixed material layer.
  • step 12 the mixed material layer is moved back and forth 5 to 50 times so as to pass through the through hole of the screen using the selected screen of the screen exchange device, thereby minimizing the biological tissue.
  • Step 14 centrifugation may be performed at high speed (RCF 500g ⁇ 3000g) to separate the mixed material layer and the material separated therefrom.
  • Step 15 After Step 14, the material layer containing the stromal vascular fibrosis (SVF) collected at the farthest from the center of rotation can be separated.
  • SVF stromal vascular fibrosis
  • Step 2 After centrifugation, material layers such as blood urea and sap are removed.
  • Step 3 the size of the adipose tissue is reduced by scraping and tearing the adipose tissue using the screen exchange device described above, and the combination of the tissue, cells, and substances constituting the adipose tissue is separated.
  • the micronized adipose tissue is compressed to obtain tissues, cells and substances separated from the adipose tissue.
  • Step 5 Afterwards, centrifugation is performed on these materials at high speed (RCF 500g ⁇ 2000g) to separate the tissues, cells and materials separated from the adipose tissue once again.
  • Step 6 After centrifugation, the material layer containing stromal vascular fibrosis (SVF) collected farthest from the rotation center is separated.
  • SVF stromal vascular fibrosis
  • Step 7 After performing Step 3, a process of removing air mixed with fatty tissue and substances can be added.
  • a screen having a through hole of 300 ⁇ m to 4000 ⁇ m may be selected from among a plurality of screens of the screen exchange device to scratch and tear adipose tissue.
  • Step 9 the first step of minimizing adipose tissue by selecting a screen having a through hole of 3000 ⁇ m to 4000 ⁇ m, and then selecting a screen having a through hole of 2000 ⁇ m to 3000 ⁇ m to refine the adipose tissue
  • a third step of selecting a screen having a through hole of 1000 ⁇ m to 2000 ⁇ m to refine the adipose tissue and then selecting a screen having a through hole of 500 ⁇ m to 1000 ⁇ m to refine adipose tissue.
  • Four steps can be performed sequentially.
  • Step 10 After the fourth step in step 9, a fifth step of miniaturizing adipose tissue by selecting a screen having a through hole of 300 ⁇ m to 500 ⁇ m may be added.
  • Step 11 By moving the fat tissue back and forth to pass through the through hole of the screen 5 to 50 times in each step of Step 9 and Step 10, it is possible to refine the living body tissue.
  • the separated materials may be filtered with a filter or a screen having a through hole of 50 ⁇ m to 200 ⁇ m.
  • Step 13 After performing step 6, after mixing saline or distilled water with the separated material layer, a screen having a through hole of 500 ⁇ m to 1000 ⁇ m may be selected to refine the mixed material layer.
  • step 14 the material layer mixed 5 to 50 times is moved reciprocally to pass through the through hole of the screen, so that the tissues, cells and materials contained in the mixed material can be refined.
  • Step 15 centrifugation may be performed at high speed (RCF 500g ⁇ 3000g) to separate the mixed material layer and the material separated therefrom.
  • Step 16 After Step 15, the material layer containing the stromal vascular fibrosis (SVF) collected at the farthest from the rotation center can be separated.
  • SVF stromal vascular fibrosis
  • the biological tissue may include adipose tissue.

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  • Health & Medical Sciences (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Anesthesiology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Un dispositif d'échange de tamis pour réduire la taille d'un tissu biologique selon un mode de réalisation comprend: un disque comprenant une pluralité d'écrans ayant chacun au moins un trou traversant configuré pour réduire la taille d'un tissu biologique, les tamis ayant des caractéristiques de trou traversant différentes; un premier couvercle qui recouvre un premier côté du disque et comprend une première ouverture à travers laquelle passe un tissu biologique; un second couvercle qui recouvre un second côté du disque et comprend une seconde ouverture à travers laquelle passe le tissu biologique; un boîtier configuré pour recevoir le disque; et un manipulateur qui sélectionne un tamis quelconque parmi la pluralité de tamis et manipule le premier couvercle ou le second couvercle de telle sorte que le tamis sélectionné communique avec la première ouverture et la seconde ouverture.
PCT/KR2020/004773 2019-04-09 2020-04-08 Dispositif d'échange de tamis, système de réduction de taille de tissu biologique le comprenant, procédé de réduction de taille de tissu biologique l'utilisant, et procédé de séparation d'une substance cible à partir d'un tissu biologique pertinent WO2020209609A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP20788402.4A EP3954405B1 (fr) 2019-04-09 2020-04-08 Dispositif d'échange de tamis et système de réduction de taille de tissu biologique le comprenant
EP23206763.7A EP4289925A3 (fr) 2019-04-09 2020-04-08 Dispositif d'échange de tamis et système de réduction de taille de tissu biologique
CN202080005584.5A CN112867571B (zh) 2019-04-09 2020-04-08 用于减小生物组织的尺寸的筛网交换装置、系统及方法
JP2021544086A JP7201830B2 (ja) 2019-04-09 2020-04-08 スクリーン交換装置、これを含む生体組織微細化システム、これを使用する生体組織微細化方法及びこれに関する生体組織からターゲット物質を分離する方法
US17/281,999 US20210353837A1 (en) 2019-04-09 2020-04-08 Screen exchange device, biological tissue size reduction system comprising same, biological tissue size reduction method using same, and method for separating target substance from relevant biological tissue

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20190041337 2019-04-09
KR10-2019-0041337 2019-04-09
KR10-2019-0131097 2019-10-22
KR1020190131097A KR102202861B1 (ko) 2019-04-09 2019-10-22 스크린 교환 장치, 이를 포함하는 생체조직 미세화 시스템, 이를 이용하는 생체조직 미세화 방법 및 이와 관련된 생체조직으로부터 타겟 물질을 분리하는 방법

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5480553A (en) 1992-02-12 1996-01-02 Mitsubishi Rayon Co., Ltd. Hollow fiber membrane module

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002259017B2 (en) * 2001-04-26 2007-08-16 Boston Biomedica, Inc. Multichamber device and uses thereof for processing of biological samples
JP2007111653A (ja) * 2005-10-21 2007-05-10 Jsr Corp 流路内蔵基板および流路内蔵基板の流体制御方法
JP2009183149A (ja) * 2008-02-01 2009-08-20 Olympus Corp 生体組織洗浄装置
EP3189129B1 (fr) * 2014-09-04 2024-02-07 Becton, Dickinson and Company Dispositifs et procédés de dissociation d'un échantillon de tissu biologique
KR102051223B1 (ko) * 2017-09-05 2019-12-03 이준석 생체조직 미세화 장치

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5480553A (en) 1992-02-12 1996-01-02 Mitsubishi Rayon Co., Ltd. Hollow fiber membrane module

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