WO2016167515A1 - Dispositif de traitement d'étalement d'échantillon liquide - Google Patents

Dispositif de traitement d'étalement d'échantillon liquide Download PDF

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Publication number
WO2016167515A1
WO2016167515A1 PCT/KR2016/003710 KR2016003710W WO2016167515A1 WO 2016167515 A1 WO2016167515 A1 WO 2016167515A1 KR 2016003710 W KR2016003710 W KR 2016003710W WO 2016167515 A1 WO2016167515 A1 WO 2016167515A1
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WO
WIPO (PCT)
Prior art keywords
smearing
filter container
liquid sample
container
filter
Prior art date
Application number
PCT/KR2016/003710
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English (en)
Korean (ko)
Inventor
전상균
Original Assignee
(주)셀트라존
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (주)셀트라존 filed Critical (주)셀트라존
Publication of WO2016167515A1 publication Critical patent/WO2016167515A1/fr

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q

Definitions

  • the present invention relates to a liquid sample smearing treatment device, and in particular, when performing the liquid smearing of the liquid sample, the solution liquid and the cell are easily separated and the slide smearing process is easily performed automatically, as well as the suction pressure, the suction time,
  • the present invention relates to a liquid cell smear processing apparatus for maximizing the precision of a test using a liquid sample by controlling or controlling the smear pressure or the smearing time.
  • cells of the body to be examined are collected, and the collected cells are stored in a solution container containing a cell solution solution. Is adopted.
  • foreign matter may be contained in addition to the cells collected in the solution container, in which case it is not easy to separate the foreign matter and cells, a separate tool for separation was required.
  • a smearing device having an improved structure has been proposed to facilitate the separation of the liquid cells and the solution solution, and to be easily performed.
  • Patent Document 1 Korean Patent Registration No. 0887590 (2009.03.02.)
  • the present invention for solving this problem is easy to separate the solution solution and the cell when performing the slide smearing of the liquid sample mixed with the cell in the solution liquid, the slide smearing process is automatically made easily, suction of the solution liquid
  • the purpose is to maximize the precision of the inspection using the liquid sample by adjusting or controlling the pressure, suction time, smear pressure, or smear time.
  • a liquid sample is accommodated, the main body portion is inserted into the filter container configured to be separated or combined at both ends;
  • the main body portion is installed to move up and down, the filter container is connected and fixed, the basket member for separating or coupling the filter container according to the up and down movement, and the body portion is moved in the transverse direction
  • a smearing portion including a slide member for performing a smearing operation in accordance with the filter contact to the filter container, and embedded in the body portion
  • a plurality of actuators configured to provide driving force for raising, lowering, and lateral movement of the smearing portion, and suction means connected to the basket member by a hose and suctioning and discharging the solution liquid contained in the filter container.
  • a driving unit to be provided and the main body unit, and during the smearing operation of the smearing unit, selection of suction pressure, suction time, smearing pressure or smearing time is performed.
  • the control unit for controlling operation of the drive section it is possible to provide a smear processing apparatus of the liquid sample characterized in that comprises a.
  • the filter container is composed of a plurality of first container and the second container which is detachably attached to each other, the inner side of the second container is provided with a filter member which is in contact with the slide member during the smearing of the smearing portion; It is possible to provide a smear treatment apparatus for a liquid sample, characterized in that.
  • the body portion may provide a liquid sample smear treatment apparatus further comprises a receiving portion for receiving the solution solution guided and discharged by the hose from the suction means.
  • the basket member is mounted so that one or more fixing holes into which one end of the filter container is inserted and fixed, and positioned inside the fixing hole, and connected to one side by the suction means and a hose, and the other side by the suction means. It is possible to provide a liquid sample smear treatment apparatus comprising a connecting member connected to one end of the filter container and connected to allow the suction of the solution solution contained in the filter container.
  • the basket member is connected by a hose to the suction means to discharge the solution solution guided along the guide groove and the guide groove for guiding the leaking solution liquid when the filter container is separated or coupled to the upper
  • a smear treatment apparatus for a liquid sample characterized in that it further comprises a drainage hole.
  • the fixing hole is a fixing protrusion for coupling the filter container to the inner circumferential surface by a slide coupling method, and a drain groove for guiding the leaking solution liquid to the upper portion of the basket member when the filter container is separated or coupled to the outer circumference. It is possible to provide a smear processing apparatus for a liquid sample, characterized in that comprising a.
  • the connecting member is a fitting nipple connected by a suction means and a hose, a reducer rotatably connected along the outer circumferential direction of the fitting nipple, connected to the reducer, and one end of the filter container is closely connected and connected. And a ring-shaped buffer member seated inside the connection member for tight connection between the connection member and the filter container. Can be.
  • connection member may provide a liquid sample smear treatment apparatus further comprises a bearing member to enable the connection member to rotate smoothly from the inside of the fixing hole.
  • the bearing member is a liquid sample smear processing apparatus characterized in that it comprises an elastic cover coupled to one end of the connecting member, and a nut ring, needle bearing and washer laminated on the inside of the elastic cover Can be provided.
  • the bearing member may be provided with a smear processing apparatus for a liquid sample, characterized in that supported by a ring-shaped snap ring coupled to the inside of the insertion hole.
  • the suction means may be provided with a liquid sample smear treatment apparatus comprising at least one discharge pipe for discharging the sucked solution liquid to the outside during the smearing operation of the smear portion.
  • control unit is provided in the main body portion, the display unit for displaying a set value or a predetermined user interface for the suction pressure, suction time, smearing pressure or smearing time in the smearing operation of the smearing portion, the display, the display It is possible to provide a liquid sample smear processing apparatus comprising a plurality of button units for performing the set value displayed in the unit, the overall operation of the predetermined user interface and the on / off control of the power.
  • the present invention when performing the slide smearing of the liquid sample mixed with the cells in the solution liquid, separation of the solution and the cell is easy, and the slide smearing process is automatically made automatically, the suction pressure, suction of the solution liquid
  • the suction pressure, suction of the solution liquid By selectively adjusting or controlling the time, the smearing pressure or the smearing time, there is an effect of maximizing the accuracy of the inspection using the liquid sample.
  • FIG. 1 is a block diagram schematically showing the overall configuration of the liquid sample smear processing apparatus according to an embodiment of the present invention.
  • Figure 2 is a perspective view schematically showing a liquid sample smear processing apparatus according to an embodiment of the present invention.
  • FIG. 3 is a plan view showing a liquid sample smear processing apparatus according to an embodiment of the present invention.
  • Figure 4 is a front view showing a liquid sample smear processing apparatus according to an embodiment of the present invention.
  • Figure 5 is a side cross-sectional view showing a liquid sample smear device according to an embodiment of the present invention.
  • Figure 6 is an enlarged view showing the smear portion of the liquid sample smear processing apparatus according to an embodiment of the present invention.
  • FIG. 7 is a view showing a basket member of the liquid sample smear processing apparatus according to an embodiment of the present invention.
  • FIG. 8 is an exemplary view showing an operating state of the liquid sample smear processing apparatus according to an embodiment of the present invention.
  • filter container 11 the first container
  • main body 110 insertion hole
  • fixing protrusion 213 drain groove
  • connecting member 221 fitting nipple
  • FIG. 1 is a block diagram schematically showing the overall configuration of the liquid sample smear processing apparatus according to an embodiment of the present invention
  • Figure 2 is a perspective view schematically showing a liquid sample smear processing apparatus according to an embodiment of the present invention
  • Figure 3 is a plan view showing a liquid sample smear processing apparatus according to an embodiment of the present invention.
  • the liquid sample smear processing apparatus is the body portion 100 is inserted into the filter container 10, the liquid sample is accommodated, and is installed in the body portion 100, the filter container Is provided in the driving unit 300 and the main body 100, which is configured to provide a driving force for the smearing operation of the smearing portion 200 and the smearing portion 200 by the contact of the (10), It is configured to include a control unit 400 for controlling the operation of the drive unit 300 according to the smearing operation of the smear unit 200.
  • the main body 100 serves as a housing in which each component of the liquid sample smear processing apparatus of the present invention is mounted.
  • the main body part 100 is formed in a hexahedral shape, and at least one insertion hole 110 penetrates upward and downward to insert the filter container 10 thereon.
  • the filter container 10 is inserted through 110.
  • the liquid sample is a liquid cell obtained by dispersing the cells collected from the body part to be examined in a cell solution to test for abnormalities in the body.
  • the filter container 10 is the liquid sample is accommodated, both ends are configured to be separated or combined, preferably the first container 11 and the second container configured to be detachable to each other up and down ( 12) is composed of a plurality.
  • the filter member 13 is provided inside the second container 12 to perform the smearing of cells by the contact of the smearing part 200 when smearing the smearing part 200.
  • the filter member 13 is preferably made of a membrane filter (membrane filter) for filtering the cells when the liquid suction of the filter container 10 according to the smearing operation.
  • the filter member 13 is configured to serve to filter the cells dispersed in the solution liquid from the liquid sample when the liquid sample is received in the filter container 10 by the drive unit 300.
  • Figure 4 is a front view showing a liquid sample smear processing apparatus according to an embodiment of the present invention.
  • the main body 100 is a receiving portion connected by a hose to receive and guide the liquid sample or solution leaked from the filter container 10 when the smearing of the smearing portion 200, the solution ( As further comprises 120, it is possible to collect and easily discard the liquid leaking during the smearing operation.
  • Figure 5 is a side cross-sectional view showing a liquid sample smearing apparatus according to an embodiment of the present invention
  • Figure 6 is an enlarged view showing the smearing portion of the liquid sample smear processing apparatus according to an embodiment of the present invention
  • Figure 7 is an embodiment of the present invention Figure showing a basket member of the liquid sample smear processing apparatus according to the example.
  • the smear portion 200 is provided in the main body portion 100 when performing the slide smearing of the liquid sample in which the cells are mixed in the solution liquid, separation of the solution liquid and the cell is easy, slide smear It makes the process easy and automatic.
  • the smear 200 is a basket member 210 which is installed to move up and down inside the main body 100 and a slide member 240 which is installed to be movable in the lateral direction. It is configured to include).
  • the basket member 210 is installed to be movable up and down in the longitudinal direction in accordance with the driving of the driving unit 300 inside the main body unit 100, and the filter container 10 at the top thereof.
  • one or more fixing holes 211 are formed in the upper portion of the basket member 210, one end of the filter container 10 inserted through the insertion hole 110 is configured to be inserted and fixed, preferably As the fixing protrusion 212 protrudes from the inner circumferential surface of the fixing hole 211, one end of the filter container 10, that is, the second container 12 is inserted into the fixing hole 211, and the filter container 10 is formed. Is rotated from the inside of the fixing hole 211, the end of the filter container 10 is fixed to the fixing projection 212 side, whereby, the filter container 10 from the inside of the fixing hole 211 The slide coupling method is firmly and easily fixed.
  • the drain groove 213 for guiding the liquid sample or solution liquid leaking from the filter container 10 to the upper portion of the basket member 210 is configured.
  • the second container 12 corresponding to one end of the filter container 10 is easily locked to the fixing protrusion 212 by rotation from the inside of the fixing hole 211, and the filter by the drain groove 213.
  • the liquid sample or the solution liquid leaking from the container 10 is guided and discharged through the drain groove 213 without being accumulated in the fixing hole 211 side.
  • the connecting member 220 is connected to the driving unit 300 on one side, the other side of the filter container 10 to enable the suction of the solution liquid of the liquid sample contained in the filter container 10 by the suction of the driving unit 300 One end is connected and connected.
  • the connecting member 220 is a fitting nipple 221 connected to the driving unit 300, a reducer 222 that is rotatably connected along the outer circumferential direction of the fitting nipple 221 is connected to the reducer 222 ,
  • One end of the filter container 10 is placed inside the connection member 223 for a close connection between the tubular connection member 223 and the connection member 223 and the filter container 10 in close connection and connection. It is configured to include a ring-shaped buffer member 224.
  • the filter container 10 is maintained in close connection with the connecting member 220, and at the same time, the filter container 10 is easily interlocked and connected to the filter container 10. The connection is convenient.
  • connection member 220 is configured to further include a bearing member 230 at one end thereof to enable the connection member 220 to smoothly rotate from the inside of the fixing hole 211.
  • the bearing member 230 is an elastic cover 235 is coupled to one end of the connecting member 220, the nut ring 231, respectively configured to be laminated on the inner side of the elastic cover 235, the needle bearing 232 And one or more washers 233 stacked on both ends of the needle bearing 232.
  • the filter container 10 when the filter container 10 is inserted into and fixed to the insertion hole 110, the wear or damage due to friction of the connecting member 200 may be reduced by the elastic cover 235, and the nut ring 231 may be used. ), The filter container 10 rotates due to the interlocking of the needle bearings 232, and the connection member 220 in contact with the filter container 10 smoothly rotates from the inside of the insertion hole 110. Accordingly, the filter container 10 can be easily attached and detached, and when the filter container 10 is attached or detached, the wear of the filter container 10, the insertion hole 110 and the connecting member 220 is minimized, and accordingly, Breakage rate can be reduced.
  • the bearing member 230 includes a ring-shaped snap ring 234 coupled to the inner side of the insertion hole 110 in an interference fit manner, so that the bearing member 230 is inserted into the hole by the snap ring 234. While easily supported from the inside of the 110, the connection member 220 is easily and easily supported from the inside of the insertion hole 110.
  • the slide member 240 is installed to be movable in the transverse direction in the main body portion 100, the inner side of the slide glass is inserted into the sample or the smear of the cell is inserted, the basket member 210 is raised, lowered As a result, in accordance with the contact of the filter member 13 of the filter container 10 to move horizontally, it is configured to be connected to the drive unit 300 to perform the smear.
  • Such a basket member 210 is a guide sample 214 for guiding the leaked liquid sample or solution solution when the filter container 10 is separated or coupled to the upper portion or a liquid sample guided along the guide groove 214 or It further comprises a drain hole 215 for discharging the solution liquid to the outside.
  • the guide groove 214 is a basket member for the liquid sample or solution that flows out or falls from the filter container 10 to the upper portion of the basket member 210 when the filter container 10 is attached or detached to the insertion hole 110 side. It is configured on the upper portion of the basket member 210 to fall to the drainage hole 215 side of (210).
  • the drainage hole 215 is formed in the front portion of the basket member 210, is connected to the receiving portion 120 by a hose, the liquid sample or solution liquid guided through the guide groove 214 receiving portion ( To guide and discharge.
  • the driving unit 300 is built into the main body 100 to collect the cells from the filter container 10 according to the smearing operation of the smearing unit 200, and drive force for raising, lowering and lateral movement of the smearing unit 200. It is configured to provide a role.
  • the drive unit 300 is built in the main body 100, a plurality of actuators 310 configured to be connected to the basket member 210 to move up and down, and connected to the basket member 210 by a hose. And, it comprises a suction means 320 for sucking and discharging the solution solution contained in the filter container (10).
  • the actuator 310 is provided in the main body portion 100, and is configured to connect the basket member 210 and the slide member 240 in the longitudinal and transverse directions, respectively, to move up, down and horizontally,
  • it may be composed of a motor and a plurality of shafts or pinions which are driven by the power of the motor to move the basket member 210 and the slide member 240 up, down and laterally.
  • the suction means 320 is installed in the main body portion 100, and is connected to the basket member 210 of the smearing portion 200 and the hose, preferably in accordance with the piston operation of the cylinder, the filter container 10 It is configured to inhale the liquid sample contained in).
  • the suction means 320 comprises one or more discharge pipes 321 for discharging the solution liquid sucked from the filter container 10 to the outside during the smearing operation of the smearing portion 200, the suction The solution liquid can be easily discharged to the outside.
  • the control unit 400 is provided in the main body unit 100, and during the smearing operation of the smearing unit 200, the driving unit 300 according to a user's setting selected from suction pressure, suction time, smearing pressure, or smearing time. It is configured to play a role in controlling the operation of.
  • the control unit 400 is provided in the main body unit 100, and when the smearing operation of the smearing unit 200 displays a set value or a predetermined user interface for suction pressure, suction time, smearing pressure or smearing time as an image.
  • the display unit 410 includes a plurality of button units 420 configured to perform on / off control of the overall operation and power of the set value and the preset user interface displayed on the display unit 410.
  • the user can selectively adjust or control the suction pressure, the suction time, the smearing pressure or the smearing time of the solution liquid, and as a result, it is possible to maximize the precision of the inspection performed using the liquid sample.
  • the button unit 420 is composed of a power on / off button, a start button, a stop button, a mode setting button, a reset button of the driving unit 300, when smearing the smearing unit 200, the container filter 10
  • the suction time or the slide member 240 of the smear portion 200 it may be configured to control the user setting or user interface of the value for the smear pressure or smearing time.
  • FIG. 8 is an exemplary view showing an operating state of the liquid sample smear processing apparatus according to an embodiment of the present invention.
  • the smear processing apparatus of the liquid sample of the present invention inserts the filter container 10 containing the liquid sample through the insertion hole 110 of the main body portion 100.
  • one end of the filter container 10, that is, the second container 12 is inserted into the fixing hole 211 of the basket member 210 located on the same center line as the insertion hole 110 of the connection member 220 In contact with the connecting member 223 and interconnected and connected, preferably as the second container 12 of the filter container 10 is seated in the buffer member 224, a more intimate connection and connection state is maintained.
  • connection member 220 is rotated smoothly from the inside of the fixing hole 211 while maintaining a close contact or connection state with the filter container 10 by the bearing member 230 when the filter container 10 rotates. Accordingly, as a result, not only the attachment and detachment of the filter container 10 is easy, but also the wear or damage caused by friction can be minimized.
  • Suction means 320 is operated to suck the liquid contained in the filter container 10 according to a predetermined pressure and a predetermined time, thereby filtering the cells in the filter member 13, and at the same time, suction means 320 The liquid sucked from) is discharged to the outside through the discharge pipe 321.
  • the basket member 210 moves downward according to the driving of the actuator 310 of the driving unit 300, the first container 11 of the filter containers 10 is seated on the insertion hole 110. As the second container 12 is separated from the first container 11, the filter member 13 of the second container 12 is exposed to the outside.
  • the slide member 240 is laterally moved by the actuator 310, the slide member 240 is positioned in the same line as the upper portion of the filter member 13, and the basket member 210 is then placed on the user for the smearing pressure and the smearing time.
  • the control unit 400 moves up and down, and as the filter member 13 is contacted and removed on the slide glass of the slide member 240, the slide member 240 Cells are smeared.
  • the slide member 240 When the smear is completed on the slide member 240, the slide member 240 returns to its original position, and the basket member 210 moves upward, as the second container 12 moves into the insertion hole 110. As it is coupled with the first container 11 seated again, the smearing is completed.
  • the present invention configured as described above is easy to separate the solution liquid and the cell when performing the slide smearing of the liquid sample mixed with the cell in the solution liquid, the slide smearing process is automatically made automatically, the suction pressure of the solution liquid, the suction time
  • the invention has the effect of maximizing the precision of the inspection using the liquid sample.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention concerne un dispositif de traitement d'étalement d'échantillon liquide. En particulier, la présente invention peut fournir un dispositif de traitement d'étalement d'un échantillon liquide comprenant : une partie de corps principal ; une partie d'étalement ; une partie d'entraînement ; et une partie de commande. La partie de corps principal reçoit un échantillon liquide et comporte un récipient de filtre inséré en son sein, le récipient de filtre étant conçu de sorte que ses deux extrémités puissent être séparées de celui-ci ou accouplées à celui-ci. La partie d'étalement comprend : un élément de panier qui est installé à l'intérieur de la partie de corps principal de manière à être mobile verticalement, auquel est raccordé et fixé le récipient de filtre, et qui permet au récipient de filtrage d'être séparé de celui-ci ou accouplé à celui-ci selon le mouvement vertical ; et un élément coulissant qui est installé dans la partie de corps principal de manière à être mobile dans la direction transversale et qui effectue une opération d'étalement en fonction du contact d'un filtre avec le récipient de filtre lorsque le récipient de filtre est séparé par le biais du mouvement vertical de l'élément de panier. La partie d'entraînement comprend: une pluralité d'actionneurs qui sont intégrés à la partie de corps principal et qui sont reliés de façon à fournir de la puissance d'entraînement pour le déplacement vertical et latéral de la partie d'étalement ; et un moyen d'aspiration qui est relié à l'élément de panier au moyen d'un tuyau et qui aspire et évacue une solution reçue dans le récipient de filtre. La partie de commande est agencée dans la partie de corps principal et, pendant l'opération d'étalement de la partie d'étalement, commande une opération de la partie d'entraînement en fonction d'un réglage d'utilisateur quelconque sélectionné parmi une pression d'aspiration, un temps d'aspiration, une pression d'étalement et un temps d'étalement.
PCT/KR2016/003710 2015-04-14 2016-04-08 Dispositif de traitement d'étalement d'échantillon liquide WO2016167515A1 (fr)

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KR10-2015-0052536 2015-04-14
KR1020150052536A KR101579717B1 (ko) 2015-04-14 2015-04-14 액상검체 도말 처리장치

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WO2020050503A1 (fr) * 2018-09-08 2020-03-12 (주)바이오다인 Dispositif de traitement de cellules exfoliantes

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CN108168972A (zh) * 2015-12-30 2018-06-15 夏周阳 一种临床检验用涂片器
KR101685459B1 (ko) * 2016-03-11 2016-12-13 (주)알엠바이오 피펫이 포함된 시료용기를 이용한 자동 도말장치
CN105784440B (zh) * 2016-03-11 2018-10-09 厦门信道生物技术有限公司 一种浓缩涂片装置
KR101997193B1 (ko) * 2017-06-13 2019-07-05 (주)코아바이오텍 검체의 도말 처리 방법
KR102273535B1 (ko) * 2019-08-14 2021-07-06 주식회사 예송 액상 세포 도말 장치
KR102399224B1 (ko) * 2020-04-14 2022-05-18 주식회사 예송 액상세포 도말용 필터용기를 이용한 분산 도말 방법
KR102375491B1 (ko) * 2020-05-22 2022-03-17 주식회사 티에스티이 세포도말용 진공 흡입 장치

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JP2003279453A (ja) * 2002-01-18 2003-10-02 Sysmex Corp 塗抹標本作製装置
KR101019085B1 (ko) * 2010-10-19 2011-03-07 (주)패스텍 세포 채취용 필터 용기
KR101037239B1 (ko) * 2011-04-27 2011-05-25 문상호 액상세포의 도말장치
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Publication number Priority date Publication date Assignee Title
WO2020050503A1 (fr) * 2018-09-08 2020-03-12 (주)바이오다인 Dispositif de traitement de cellules exfoliantes
JP2021536008A (ja) * 2018-09-08 2021-12-23 バイオダイン カンパニー リミテッドBiodyne Co., Ltd. 剥離細胞の処理装置
JP7094592B2 (ja) 2018-09-08 2022-07-04 バイオダイン カンパニー リミテッド 剥離細胞の処理装置

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