WO2020192091A1 - 一种免疫分析仪磁清洗分离装置 - Google Patents

一种免疫分析仪磁清洗分离装置 Download PDF

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Publication number
WO2020192091A1
WO2020192091A1 PCT/CN2019/111366 CN2019111366W WO2020192091A1 WO 2020192091 A1 WO2020192091 A1 WO 2020192091A1 CN 2019111366 W CN2019111366 W CN 2019111366W WO 2020192091 A1 WO2020192091 A1 WO 2020192091A1
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Prior art keywords
cleaning
reaction cup
magnet
magnetic
running path
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PCT/CN2019/111366
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English (en)
French (fr)
Inventor
苗新利
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苏州长光华医生物医学工程有限公司
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Publication of WO2020192091A1 publication Critical patent/WO2020192091A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04—Cleaning involving contact with liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04—Cleaning involving contact with liquid
    • B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Definitions

  • the patent with document number CN109433686A discloses a magnetic bead cleaning device and a fully automatic chemiluminescence immunoassay analyzer.
  • the magnetic bead cleaning device includes a reaction cup transport assembly, a cleaning needle assembly, a magnet assembly and at least two cleaning positions.
  • the assembly includes a magnet corresponding to each cleaning position.
  • the magnet can adsorb the magnetic beads in the reaction cup on the wall of the reaction cup, and the magnets corresponding to any two adjacent cleaning positions are located in the conveying path of the reaction cup.
  • the magnetic bead cleaning device can make the reaction cup change the cleaning position, the magnetic beads move from one wall of the reaction cup to the wall opposite to the wall to clean the magnetic beads, but the magnetic beads are moving from one of the reaction cups.
  • a magnetic cleaning and separating device for an immune analyzer comprising a reaction cup conveying assembly, a cleaning needle assembly, a magnet assembly and several cleaning positions;
  • the reaction cup conveying component is used to convey the reaction cup to different cleaning positions
  • the cleaning needle assembly is used for injecting cleaning liquid into the reaction cup in the cleaning position and sucking the cleaning liquid from the reaction cup;
  • the magnet assembly includes two opposing magnet pairs arranged on both sides of the running path of the reaction cup and a single magnet arranged on one side of the running path of the reaction cup.
  • the pair of magnets corresponds to the cleaning position, and the single magnet is located in the cleaning position.
  • the magnet is a square magnet, and the magnetic pole direction of the magnet is perpendicular to the running path of the reaction cup or perpendicular to the tangent of the running path.
  • the polarities of the sides of the two adjacent magnets close to the reaction cup running path are the same.
  • the single magnet closest to the last cleaning position in the direction of the cuvette running path is 1-3 mm higher than the magnet corresponding to the last cleaning position on the same side of the adjacent cuvette running path, and the single magnet is higher than The height of other magnets is 3-5mm lower.
  • the minimum distance between the two magnets of the magnet pair is 6-10 mm.
  • the reaction cup conveying assembly includes a fixed part and a moving part.
  • the fixed part is provided with a reaction cup running groove
  • the moving part is provided with a receiving part for accommodating the reaction cup.
  • the moving part can carry the reaction cup in the reaction cup. Move in the running trough.
  • the fixed part and the moving part are respectively a cylinder and a disc rotatably arranged in the cylinder, the cylinder is provided with a ring-shaped reaction cup running groove, and a circle is arranged on the disc in an equal diameter
  • the reaction cup contains the hole.
  • the magnet group set before each cleaning position is composed of two magnets and runs close to the reaction cup.
  • the magnet arrangement and the magnet polarity on the side of the running slot of the reaction cup near the running slot of the reaction cup are: S, S, N, N, S, S, and the reaction far away from the center of the running slot of the reaction cup.
  • the arrangement of the magnets on the side of the cup running slot and the magnet polarity on the side close to the running slot of the reaction cup are: N, N, S, S, N, N.
  • the height of the other magnets is 8-12 mm
  • the magnetic induction intensity of the center point of the end face of the other magnets near the side of the running path of the reaction cup is at least 620mT.
  • the present invention also provides an immune analyzer, including the above-mentioned magnetic cleaning and separation device for the immune analyzer.
  • the present invention provides a magnetic cleaning and separating device for an immune analyzer, which is provided with a pair of magnets located on both sides of the running path of the reaction cup in the cleaning position, and between two adjacent cleaning positions or in the direction of the running path of the reaction cup Before the first cleaning position, a number of magnets located on the side of the running path of the reaction cup are set, so that the magnetic beads in the reaction cup can be rotated under the action of the magnet group before the cleaning position, and the magnetic bead clusters are scattered, making the magnetic beads in the cleaning position.
  • the magnetic beads When beads are used, the unbound substances wrapped in the binding material are released, and the part of the reactants combined with the magnetic beads is better retained, achieving the purpose of fully cleaning the magnetic beads, and the high magnetic induction intensity of the magnet pair corresponding to the cleaning position Under the action of, the magnetic beads are quickly attracted to the two side walls of the reaction cup, so that the cleaning needle can avoid attracting the magnetic beads in the reaction cup to the cleaning needle when the cleaning needle is sucking the cleaning solution in a short time, ensuring the entire magnetic The time during the separation process can be as short as possible. Finally, it can be ensured that the magnetic beads in the cup can fully react with the substrate after the reaction cup enters the measurement chamber, and the substrate solution is saved.
  • the magnetic cleaning and separation device of the immunoanalyzer of the present invention uses a square magnet, and the magnetic pole direction of the magnet is perpendicular to the running path of the reaction cup or perpendicular to the tangent of the running path.
  • This design can make the magnetic beads in the reaction cup be quickly attracted to the reaction cup On the side wall of the magnetic beads, the washing effect of the magnetic beads is enhanced, and the washing needle can absorb the washing liquid in a shorter time without attracting the magnetic beads in the reaction cup into the washing needle.
  • the phase on the same side of the reaction cup running path When the minimum distance between two adjacent magnets is less than 15mm, the polarity of the two adjacent magnets near the running path of the cuvette is the same.
  • Figure 1 is a three-dimensional view of a magnetic cleaning and separating device for an immune analyzer of the present invention
  • Figure 2 is a schematic diagram of the magnet arrangement and magnetic pole arrangement of the magnetic cleaning and separation device of the immune analyzer of the present invention.
  • Reaction cup delivery assembly 11-cylinder, 12-disc, 2-cleaning needle assembly, 3-magnet assembly, 4-cleaning position, 41-first cleaning position, 42-second cleaning position, 43-third cleaning Bit, 5-reaction cup.
  • This embodiment provides a magnetic cleaning and separation device for an immune analyzer, as shown in FIG. 1, including a reaction cup conveying assembly 1, a cleaning needle assembly 2, a magnet assembly 3, and a cleaning position 4;
  • the cuvette conveying assembly 1 is used to convey the cuvettes 5 to different cleaning positions 4.
  • the cuvette conveying assembly 1 includes a fixed part and a moving part.
  • the fixed part is provided with a reaction cup running groove, which moves
  • the part is provided with a receiving part for accommodating the reaction cup 5, and the moving part can carry the reaction cup 5 to move in the reaction cup running groove;
  • the cleaning needle assembly 2 is used for injecting cleaning liquid into the reaction cup 5 in the cleaning position 4 and sucking the cleaning liquid from the reaction cup 5. It is preferable to set a corresponding cleaning needle assembly 2 for each cleaning station 4, so much A cleaning needle assembly 2 can work at the same time to improve cleaning efficiency;
  • the single magnet closest to the last cleaning position in the direction of the cuvette running path is 1-3mm higher than the magnet corresponding to the last cleaning position on the same side of the adjacent cuvette running path (for example, 1 mm). , 2mm, 3mm), and the height of the single magnet is 3-5mm lower than other magnets (such as 3mm, 4mm, 5mm).
  • a square magnet is used, and the magnetic pole direction of the magnet is perpendicular to the running path of the reaction cup or the tangent to the running path.
  • the magnetic beads in the reaction cup are adsorbed for the first time, the magnetic beads will be distributed in a plane On the side wall of the cuvette, use a magnet to adsorb again on the other side of the cuvette, and the magnetic beads will quickly converge to the center of the magnet and gather into dots, which can maximize the magnetic attraction between the magnet and the magnetic beads in the cuvette 5 and enhance The washing effect of the magnetic beads, and can make the reaction cup 5 in the cleaning position 4, the magnetic beads can be quickly attracted to the two side walls of the reaction cup 5, so that the cleaning needle in a shorter time to avoid the reaction The magnetic beads in the cup are sucked into the cleaning needle.
  • the minimum distance between two adjacent magnets on the same side of the reaction cup running groove is less than 15mm (such as 10mm, 12mm, 14mm)
  • the polarity of the two adjacent magnets near the side of the reaction cup running groove Same, otherwise two adjacent magnets will attract each other, forming a closed magnetic line of induction, which will affect the adsorption of the magnetic beads in the reaction cup.
  • the fixed part and the moving part are a cylinder 11 and a disc 12 rotatably arranged in the cylinder, respectively.
  • the cylinder 11 is provided with an annular reaction cup running groove.
  • the magnet groups set before position 4 are all two magnets.
  • the magnets on the side of the cuvette running groove near the center of the cuvette’s running groove and the magnet arrangement and magnet polarity on the side close to the cuvette running groove are: S, S , N, N, S, S, the magnet on the side of the cuvette running groove far from the center of the cuvette’s running groove and the magnet arrangement and the magnet polarity on the side close to the cuvette’s running groove are: N, N, S, S, N, N.
  • the reaction cup 5 is made to pass through the first cleaning position 41, the second cleaning position 42, and the third cleaning position 43 in sequence through the cup transport assembly 1.
  • the magnetic beads in the reaction cup 5 First, the first magnet is magnetically attracted to make the magnetic beads adsorb on the wall of the reaction cup 5, and then the reaction cup 5 passes through the second magnet, and the magnetic beads are attracted by the magnetic attraction of the magnetic beads and the second magnet On the cup wall on the other side of the reaction cup 5, then the reaction cup 5 passes through the magnet pair corresponding to the first cleaning position 41, so that the magnetic beads are adsorbed on the cup walls on both sides of the reaction cup 5.
  • the cleaning needle assembly 2 The reaction cup 5 absorbs the cleaning solution and injects new cleaning solution into the reaction cup 5.
  • the reaction cup 5 sequentially passes through the magnet group before the second cleaning position 42, the magnet pair corresponding to the second cleaning position 42, The magnet group before the first cleaning position 41 and the pair of magnets corresponding to the first cleaning position 41, and repeat the above-mentioned liquid extraction and injection process at the second cleaning position 42 and the third cleaning position 43; this way, not only makes the reaction cup
  • the magnetic beads inside realize the rotation under the action of the magnet group in front of cleaning position 4, disperse the magnetic bead clusters to effectively clean the magnetic beads, and under the action of the high magnetic induction intensity of the magnet pair corresponding to cleaning position 4, make The magnetic beads are quickly attracted to the two side walls of the reaction cup, which can prevent the magnetic beads in the reaction cup from being sucked into the washing needle when the washing needle absorbs the washing solution in a short time.
  • the height reduction of the magnet on one side of the reaction cup running tank and its adjacent magnets corresponding to the last cleaning position is mainly to pull the magnetic beads in the reaction cup to the bottom of the reaction cup to ensure that the reaction cup enters the measurement chamber
  • the magnetic beads in the cup can fully react with the substrate.
  • the height of the other magnets is 8 -12mm (such as 8mm, 10mm, 12mm)
  • the magnetic induction intensity of other magnets near the center point of the end surface of the reaction cup running path side is at least 620mT, which can ensure a good magnetic bead washing effect.
  • the magnet is a permanent magnet, such as a neodymium iron boron magnet, a samarium cobalt magnet or an alnico magnet.

Abstract

一种免疫分析仪磁清洗分离装置，包括反应杯输送组件(1)、清洗针组件(2)、磁体组件(3)和若干清洗位(4)；磁体组件包括在反应杯运行路径两侧布置的两个相对设置的磁体对和在反应杯(5)运行路径一侧布置的单个磁体，磁体对与清洗位相对应，单个磁体位于清洗位之间或者位于反应杯运行路径方向上首个清洗位之前，清洗位之间或者位于反应杯运行路径方向上首个清洗位之前在反应杯运行路径的每侧至少具有一个磁体，清洗位之间或者位于反应杯运行路径方向上首个清洗位之前的磁体中，磁体在反应杯运行路径两侧交替设置且相邻两个磁体的磁极方向相反。该磁清洗分离装置磁分离时间极短，达到了充分清洗磁珠的目的，并且能够有效避免清洗针吸到磁珠。

Description

一种免疫分析仪磁清洗分离装置 技术领域
本申请属于医疗器械技术领域，尤其是涉及一种免疫分析仪磁清洗分离装置。
背景技术
在全自动免疫检测系统中，免疫复合物的有效分离是免疫检测的关键环节，分离效果直接影响免疫检测结果。目前，在采用磁珠作为反应载体的免疫分析仪中，免疫复合物分离主要采用磁分离法，磁分离法是利用免疫磁珠同时具有易磁化能力和特异性结合能力，令其与靶标物质进行特异性结合，再借助外部磁场将结合磁珠的反应物(免疫复合物)保留，分离掉多余反应物和试剂。磁分离法的整个磁分离过程中的时间可以很短，但是现有的免疫分析仪磁清洗分离装置对免疫复合物的洗涤效果欠佳。
例如，文献号为CN109433686A的专利公开了一种磁珠清洗装置及全自动化学发光免疫分析仪，该磁珠清洗装置包括反应杯输送组件、清洗针组件、磁体组件和至少两个清洗位，磁体组件包括对应于每个清洗位设置的磁体，所述磁体能够将反应杯中的磁珠吸附于反应杯的杯壁，并且任意两个相邻的清洗位所对应的磁体位于反应杯输送路径的两侧，该磁珠清洗装置可以使反应杯转换清洗位时，磁珠从反应杯的一个壁面移动到与该壁面相对的壁面，以对磁珠进行清洗，但是磁珠在从反应杯的一个壁面移动到与该壁面相对的壁面的时间较久，极大地影响了工作效率，若磁珠未被完全吸附到反应杯壁面时即用清洗针吸附清洗液时，清洗针容易吸到反应杯内的磁珠。
发明内容
本发明要解决的技术问题是：为解决现有磁清洗分离装置的磁珠清洗效率低及清洗针容易吸到反应杯内的磁珠的不足，从而提供一种免疫分析仪磁清洗分离装置。
本发明解决其技术问题所采用的技术方案是：
一种免疫分析仪磁清洗分离装置，包括反应杯输送组件、清洗针组件、磁体组 件和若干清洗位；
所述反应杯输送组件用于将反应杯输送至不同的清洗位；
所述清洗针组件用于向清洗位内的反应杯中注入清洗液和从反应杯中吸取清洗液；
所述磁体组件包括在反应杯运行路径两侧布置的两个相对设置的磁体对和在反应杯运行路径一侧布置的单个磁体，所述磁体对与清洗位位置相对应，单个磁体位于清洗位之间或者位于反应杯运行路径方向上首个清洗位之前，相邻两个清洗位之间或者位于反应杯运行路径方向上首个清洗位之前在反应杯运行路径的每侧至少具有一个磁体，并且相邻两个清洗位之间或者位于反应杯运行路径方向上首个清洗位之前的磁体中，磁体在反应杯运行路径两侧交替设置且相邻两个磁体的磁极方向相反。
优选地，所述磁体为方形磁体，所述磁体的磁极方向与反应杯运行路径或者与运行路径的切线垂直。
优选地，反应杯运行路径同侧的相邻两个磁体的最小距离低于15mm时，所述相邻两个磁体靠近反应杯运行路径一侧的极性相同。
优选地，与反应杯运行路径方向上的最后一个清洗位最接近的单个磁体比其相邻的反应杯运行路径同侧的最后一个清洗位对应的磁体高1-3mm，且所述单个磁体比其他磁体的高度低3-5mm。
优选地，所述磁体对的两个磁体之间的最小距离为6-10mm。
优选地，所述反应杯输送组件包括固定部和移动部，所述固定部内设置有反应杯运行槽，移动部上设置有用于容纳反应杯的容纳部，移动部能够带着反应杯在反应杯运行槽中移动。
优选地，固定部和移动部分别为圆筒和转动设置在圆筒内的圆盘，所述圆筒内设置有圆环形的反应杯运行槽，所述圆盘上等径排布有一圈反应杯容纳孔。
优选地，清洗位有三个，沿着反应杯运输方向依次为第一清洗位、第二清洗位和第三清洗位，每个清洗位之前设置的磁体组均为两个磁体，靠近反应杯运行槽圆心的反应杯运行槽一侧的磁体在靠近反应杯运行槽一侧的磁体排布和磁体极性依次为：S，S，N，N，S，S，远离反应杯运行槽圆心的反应杯运行槽一侧的磁体 在靠近反应杯运行槽一侧的磁体排布和磁体极性为：N，N，S，S，N，N。
优选地，除了与反应杯运行路径方向上的最后一个清洗位最接近的单个磁体及其相邻的反应杯运行路径同侧的最后一个清洗位对应的磁体，其他磁体的高度为8-12mm，其他磁体靠近反应杯运行路径一侧的端面中心点的磁感应强度至少为620mT。
优选地，所述磁体为永磁体，优选为钕铁硼磁体、钐钴磁体或铝镍钴磁体。
本发明还提供一种免疫分析仪，包括上述的免疫分析仪磁清洗分离装置。
本发明的有益效果是：
(1)本发明提供了一种免疫分析仪磁清洗分离装置，其在清洗位设置位于反应杯运行路径两侧的磁体对，并在相邻两个清洗位之间或者位于反应杯运行路径方向上首个清洗位之前设置位于反应杯运行路径一侧的若干磁体，使得反应杯内的磁珠在清洗位前的磁体组的作用下实现了旋转，打散了磁珠团，使得在清洗磁珠时使包裹在结合物中的未结合物释放出来，较好地保留与磁珠结合的那一部分反应物，达到了充分清洗磁珠的目的，并且在清洗位对应的磁体对的高磁感应强度的作用下，使磁珠快速被吸引到反应杯的两个侧壁上，可以使清洗针在短时间内吸取清洗液时避免将反应杯内的磁珠吸到清洗针内，保证了整个磁分离过程中的时间可以尽可能的短，最后还可以保证反应杯进入测量室后杯内的磁珠可以充分与底物进行反应，并节约底物溶液。
(2)本发明的免疫分析仪磁清洗分离装置的采用方形磁体，且磁体的磁极方向与反应杯运行路径或者与运行路径的切线垂直，此设计可以使反应杯内磁珠快速吸引到反应杯的侧壁上，增强磁珠的洗涤效果，并且使清洗针在更短时间内吸取清洗液而不将反应杯内的磁珠吸到清洗针内，进一步，在反应杯运行路径同侧的相邻两个磁体的最小距离低于15mm时，该相邻两个磁体靠近反应杯运行路径一侧的极性相同，此设计可以避免反应杯运行路径同侧的相邻两个磁体的距离过小时，若靠近反应杯运行路径一侧的极性不同导致形成封闭的磁感线，最终保证了对反应杯内磁珠的有效吸附。
附图说明
下面结合附图和实施例对本申请的技术方案进一步说明。
图1是本发明免疫分析仪磁清洗分离装置的立体视图；
图2是本发明免疫分析仪磁清洗分离装置的磁体布置和磁极排布示意图。
附图标记为：
反应杯输送组件，11-圆筒，12-圆盘，2-清洗针组件，3-磁体组件，4-清洗位，41-第一清洗位，42-第二清洗位，43-第三清洗位，5-反应杯。
具体实施方式
需要说明的是，在不冲突的情况下，本申请中的实施例及实施例中的特征可以相互组合。
下面将参考附图并结合实施例来详细说明本申请的技术方案。
实施例
本实施例提供一种免疫分析仪磁清洗分离装置，如图1所示，包括反应杯输送组件1、清洗针组件2、磁体组件3和清洗位4；
所述反应杯输送组件1用于将反应杯5输送至不同的清洗位4，具体地，所述反应杯输送组件1包括固定部和移动部，所述固定部内设置有反应杯运行槽，移动部上设置有用于容纳反应杯5的容纳部，移动部能够带着反应杯5在反应杯运行槽中移动；
所述清洗针组件2用于向清洗位4内的反应杯5中注入清洗液和从反应杯5中吸取清洗液，优选为对应每个清洗工位4设置相应的清洗针组件2，这样多个清洗针组件2可以同时工作，提高清洗效率；
所述磁体组件3包括在反应杯运行路径两侧布置的两个相对设置的磁体对和在反应杯运行路径一侧布置的单个磁体，所述磁体对与清洗位4位置相对应，单个磁体位于清洗位4之间或者位于反应杯运行路径方向上首个清洗位4之前，相邻两个清洗位4之间或者位于反应杯运行路径方向上首个清洗位4之前在反应杯运行路径的每侧至少具有一个磁体，并且相邻两个清洗位4之间或者位于反应杯运行路径方向上首个清洗位4之前的磁体中，磁体在反应杯运行路径两侧交替设置且相邻两个磁体的磁极方向相反。
在本实施例中，与反应杯运行路径方向上的最后一个清洗位最接近的单个磁体比其相邻的反应杯运行路径同侧的最后一个清洗位对应的磁体高1-3mm(如1m m、2mm、3mm)，且所述单个磁体比其他磁体的高度低3-5mm(如3mm、4mm、5mm)。
在本实施例中，采用方形磁体，所述磁体的磁极方向与反应杯运行路径或者与运行路径的切线垂直，当反应杯内的磁珠第一次被吸附时，磁珠会以面状分布于反应杯侧壁，在反应杯另一侧使用磁体再次吸附，磁珠会快速汇聚到磁体的中心并聚集成点状，这样可以使磁体与反应杯5内磁珠的磁力吸引最大化，增强磁珠的洗涤效果，并且可以使反应杯5在清洗位4时，将磁珠快速吸引到反应杯5的两个侧壁上，以使清洗针在更短时间内吸取清洗液时避免将反应杯内的磁珠吸到清洗针内。
在本实施例中，反应杯运行槽同侧的相邻两个磁体的最小距离低于15mm(如10mm、12mm、14mm)时，该相邻两个磁体靠近反应杯运行槽一侧的极性相同，否则相邻两个磁体会相互吸引，形成封闭的磁感线，影响对反应杯内磁珠的吸附。
如图1和图2所示，固定部和移动部分别为圆筒11和转动设置在圆筒内的圆盘12，所述圆筒11内设置有圆环形的反应杯运行槽，所述圆盘12上等径排布有一圈反应杯容纳孔，清洗位4有三个，沿着反应杯运输方向依次为第一清洗位41、第二清洗位42和第三清洗位43，每个清洗位4之前设置的磁体组均为两个磁体，靠近反应杯运行槽圆心的反应杯运行槽一侧的磁体在靠近反应杯运行槽一侧的磁体排布和磁体极性依次为：S，S，N，N，S，S，远离反应杯运行槽圆心的反应杯运行槽一侧的磁体在靠近反应杯运行槽一侧的磁体排布和磁体极性为：N，N，S，S，N，N。
具体实施时，通过应杯输送组件1使反应杯5依次经过第一清洗位41、第二清洗位42和第三清洗位43，在经过第一清洗位41之前，反应杯5内的磁珠先与第一个磁体实现磁力吸引以使磁珠吸附在反应杯5一侧的杯壁上，接着反应杯5经过第二个磁体，通过磁珠与第二个磁体的磁力吸引使得磁珠吸附在反应杯5另一侧的杯壁上，然后反应杯5经过第一清洗位41对应的磁体对，使得磁珠吸附在反应杯5两侧的杯壁上，此时，清洗针组件2从该反应杯5中吸取清洗液，并向该反应杯5中注入新的清洗液，之后，该反应杯5依次经过第二清洗位42之前的磁体组、 第二清洗位42对应的磁体对、第一清洗位41之前的磁体组及第一清洗位41对应的磁体对，并在第二清洗位42和第三清洗位43处重复上述的抽液和注液过程；如此，不仅使得反应杯内的磁珠在清洗位4前的磁体组的作用下实现了旋转，打散了磁珠团，以有效清洗磁珠，并且在清洗位4对应的磁体对的高磁感应强度的作用下，使磁珠快速被吸引到反应杯的两个侧壁上，可以使清洗针在短时间内吸取清洗液时避免将反应杯内的磁珠吸到清洗针内。另外，最后一个清洗位对应的反应杯运行槽单侧的磁体及其相邻磁体的高度降低主要是将反应杯中的磁珠逐渐下拉至反应杯杯底处，以保证反应杯进入测量室后杯内的磁珠可以充分与底物进行反应。
进一步，本实施例中除了与反应杯运行路径方向上的最后一个清洗位最接近的单个磁体及其相邻的反应杯运行路径同侧的最后一个清洗位对应的磁体，其他磁体的高度为8-12mm(如8mm、10mm、12mm)，其他磁体靠近反应杯运行路径一侧的端面中心点的磁感应强度至少为620mT，该磁感应强度可以保证良好的磁珠洗涤效果。
在本实施例中，所述磁体对的两个磁体之间的最小距离为6-10mm(如6mm、8mm、10mm)，此设计可以进一步使磁体对构成的磁场流对反应杯5内的磁珠产生足够的吸附能力。
在本实施例中，磁体为永磁体，例如为钕铁硼磁体、钐钴磁体或铝镍钴磁体。
以上述依据本申请的理想实施例为启示，通过上述的说明内容，相关工作人员完全可以在不偏离本项申请技术思想的范围内，进行多样的变更以及修改。
本项申请的技术性范围并不局限于说明书上的内容，必须要根据权利要求范围来确定其技术性范围。
发明概述
技术问题
问题的解决方案
发明的有益效果

Claims (10)

  1. 一种免疫分析仪磁清洗分离装置，其特征在于，
    包括反应杯输送组件(1)、清洗针组件(2)、磁体组件(3)和若干清洗位(4)；所述反应杯输送组件(1)用于将反应杯(5)输送至不同的清洗位(4)；
    所述清洗针组件(2)用于向清洗位(4)内的反应杯(5)中注入清洗液和从反应杯(5)中吸取清洗液；所述磁体组件(3)包括在反应杯运行路径两侧布置的两个相对设置的磁体对和在反应杯运行路径一侧布置的单个磁体，所述磁体对与清洗位(4)位置相对应，单个磁体位于清洗位(4)之间或者位于反应杯运行路径方向上首个清洗位(4)之前，相邻两个清洗位(4)之间或者位于反应杯运行路径方向上首个清洗位(4)之前在反应杯运行路径的每侧至少具有一个磁体，并且相邻两个清洗位(4)之间或者位于反应杯运行路径方向上首个清洗位(4)之前的磁体中，磁体在反应杯运行路径两侧交替设置且相邻两个磁体的磁极方向相反。
  2. 根据权利要求1所述的免疫分析仪磁清洗分离装置，其特征在于，所述磁体为方形磁体，所述磁体的磁极方向与反应杯运行路径或者与运行路径的切线垂直。
  3. 根据权利要求1或2所述的免疫分析仪磁清洗分离装置，其特征在于，反应杯运行路径同侧的相邻两个磁体的最小距离低于15mm时，所述相邻两个磁体靠近反应杯运行路径一侧的极性相同。
  4. 根据权利要求1-3任一项所述的免疫分析仪磁清洗分离装置，其特征在于，与反应杯运行路径方向上的最后一个清洗位最接近的单个磁体比其相邻的反应杯运行路径同侧的最后一个清洗位对应的磁体高1-3mm，且所述单个磁体比其他磁体的高度低3-5mm。
  5. 根据权利要求1-4任一项所述的免疫分析仪磁清洗分离装置，其特征在于，所述磁体对的两个磁体之间的最小距离为6-10mm。
  6. 根据权利要求1-5任一项所述的免疫分析仪磁清洗分离装置，其特征在于，除了与反应杯运行路径方向上的最后一个清洗位最接近的单个磁体及其相邻的反应杯运行路径同侧的最后一个清洗位对应的磁体，其他磁体的高度为8-12mm，其他磁体靠近反应杯运行路径一侧的端面中心点的磁感应强度至少为620mT。
  7. 根据权利要求1-6任一项所述的免疫分析仪磁清洗分离装置，其特征在于，所述反应杯输送组件(1)包括固定部和移动部，所述固定部内设置有反应杯运行槽，移动部上设置有用于容纳反应杯(5)的容纳部，移动部(12)能够带着反应杯(5)在反应杯运行槽中移动；所述固定部(11)和移动部(12)进一步为圆筒(11)和转动设置在圆筒内的圆盘(12)，所述圆筒(11)内设置有圆环形的反应杯运行槽，所述圆盘(12)上等径排布有一圈反应杯容纳孔。
  8. 根据权利要求7所述的免疫分析仪磁清洗分离装置，其特征在于，清洗位(4)有三个，沿着反应杯运输方向依次为第一清洗位(41)、第二清洗位(42)和第三清洗位(43)，每个清洗位(4)之前设置的磁体组均为两个磁体，靠近反应杯运行槽圆心的反应杯运行槽一侧的磁体在靠近反应杯运行槽一侧的磁体排布和磁体极性依次为：S，S，N，N，S，S，远离反应杯运行槽圆心的反应杯运行槽一侧的磁体在靠近反应杯运行槽一侧的磁体排布和磁体极性为：N，N，S，S，N，N。
  9. 根据权利要求1-8任一项所述的免疫分析仪磁清洗分离装置，其特征在于，所述磁体为永磁体，优选为钕铁硼磁体、钐钴磁体或铝镍钴磁体。
  10. 一种免疫分析仪，其特征在于，包括权利要求1-9任一项所述的免疫分析仪磁清洗分离装置。
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