WO2020188181A8 - Nouvelles souches de bacteries lactiques favorisant l'absorption du calcium - peptides et produits associes - Google Patents

Nouvelles souches de bacteries lactiques favorisant l'absorption du calcium - peptides et produits associes Download PDF

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Publication number
WO2020188181A8
WO2020188181A8 PCT/FR2020/050477 FR2020050477W WO2020188181A8 WO 2020188181 A8 WO2020188181 A8 WO 2020188181A8 FR 2020050477 W FR2020050477 W FR 2020050477W WO 2020188181 A8 WO2020188181 A8 WO 2020188181A8
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WIPO (PCT)
Prior art keywords
identity
strain
seq
milk
peptides
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Application number
PCT/FR2020/050477
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English (en)
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WO2020188181A1 (fr
Inventor
Benoit Cudennec
François COUTTE
Pascal Dhulster
Djamel Drider
Christophe FLAHAUT
Marc Fremont
Cyril RAVESCHOT
Maxime VAEREMANS
Original Assignee
Vf Bioscience
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR1902851A external-priority patent/FR3094014A1/fr
Priority claimed from FR1902854A external-priority patent/FR3094015A1/fr
Priority claimed from FR1902847A external-priority patent/FR3094013A1/fr
Application filed by Vf Bioscience filed Critical Vf Bioscience
Priority to AU2020243322A priority Critical patent/AU2020243322A1/en
Priority to EP20725858.3A priority patent/EP3942016A1/fr
Priority to KR1020217033727A priority patent/KR20210142692A/ko
Priority to US17/440,566 priority patent/US20230287331A1/en
Publication of WO2020188181A1 publication Critical patent/WO2020188181A1/fr
Publication of WO2020188181A8 publication Critical patent/WO2020188181A8/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/59—Compounds containing 9, 10- seco- cyclopenta[a]hydrophenanthrene ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/12—Drugs for disorders of the metabolism for electrolyte homeostasis
    • A61P3/14—Drugs for disorders of the metabolism for electrolyte homeostasis for calcium homeostasis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4732—Casein
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8103—Exopeptidase (E.C. 3.4.11-19) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/15—Peptidyl-dipeptidases (3.4.15)
    • C12Y304/15001—Peptidyl-dipeptidase A (3.4.15.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/306—Foods, ingredients or supplements having a functional effect on health having an effect on bone mass, e.g. osteoporosis prevention
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/147—Helveticus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Epidemiology (AREA)
  • Endocrinology (AREA)
  • Rheumatology (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Toxicology (AREA)

Abstract

La présente invention concerne la souche de Lactobacillus helveticus VF45A déposée à la CNCM sous le numéro d'ordre CNCM-l-5300, ses mutants et variants présentant au moins 80% d'identité, de préférence au moins 90% d'identité, de préférence au moins 95% d'identité avec le génome de ladite souche VF45A et capables de produire au moins un peptide correspondant aux séquences SEQ ID 1 à SEQ ID NO 43 et/ou capables de réduire par fermentation anaérobie le pH du lait, notamment de vache et notamment le lait de vache écrémé à une valeur sensiblement égale ou inférieure à 3,36, notamment égale à 3,32 au bout de 48 heures de fermentation à 37°C. La présente invention concerne également des peptides isolés pouvant être obtenu par fermentation du lait, notamment de vache par la souche précitée. La présente invention concerne également la souche de Lactobacillus helveticus VFH049 déposée à la CNCM sous le numéro d'ordre CNCM-l-5403 et ses mutants et variants présentant au moins 80% d'identité, de préférence au moins 90% d'identité, de préférence au moins 95% d'identité avec le génome de ladite souche VFHD49 et capables de produire au moins un peptide correspondant aux séquences SEQ ID 44 à SEQ ID NO 86 et/ou capables d'augmenter l'absorption intestinale du calcium. La présente invention concerne également un peptide isolé, un mélange de peptides et des produits et composition associés et la souche de Lactobacillus delbrueckii ssp. bulgaricus VF50b déposée à la CNCM sous le numéro d'ordre CNCM-l-5316 et ses mutants et variants présentant au moins 80% d'identité, de préférence au moins 90% d'identité, de préférence au moins 95% d'identité avec le génome de ladite souche VF50b et capables de produire au moins un peptide correspondant aux séquences SEQ ID 87 à SEQ ID NO 199 et/ou capables d'augmenter l'absorption intestinale du calcium. La présente invention concerne également un peptide isolé, un mélange de peptides et des compositions pharmaceutique et alimentaire associées.
PCT/FR2020/050477 2019-03-20 2020-03-09 Nouvelles souches de bacteries lactiques favorisant l'absorption du calcium - peptides et produits associes WO2020188181A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2020243322A AU2020243322A1 (en) 2019-03-20 2020-03-09 Novel lactic acid bacteria strains that promote the absorption of calcium - peptides and associated products
EP20725858.3A EP3942016A1 (fr) 2019-03-20 2020-03-09 Nouvelles souches de bacteries lactiques favorisant l'absorption du calcium - peptides et produits associes
KR1020217033727A KR20210142692A (ko) 2019-03-20 2020-03-09 칼슘의 흡수를 촉진하는 신규 락트산 박테리아 균주 - 펩티드 및 연관 생성물
US17/440,566 US20230287331A1 (en) 2019-03-20 2020-03-09 Novel lactic acid bacteria strains that promote the absorption of calcium - peptides and associated products

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
FR1902851A FR3094014A1 (fr) 2019-03-20 2019-03-20 Nouvelles souches de bacteries lactiques favorisant l’absorption du calcium – peptides et produits associes
FR1902854A FR3094015A1 (fr) 2019-03-20 2019-03-20 Nouvelles souches de bacteries lactiques favorisant l’absorption du calcium – peptides et produits associes
FR1902847 2019-03-20
FR1902854 2019-03-20
FR1902847A FR3094013A1 (fr) 2019-03-20 2019-03-20 Nouvelles souches de bacteries lactiques favorisant l’absorption du calcium – peptides et produits associes
FR1902851 2019-03-20

Publications (2)

Publication Number Publication Date
WO2020188181A1 WO2020188181A1 (fr) 2020-09-24
WO2020188181A8 true WO2020188181A8 (fr) 2021-11-04

Family

ID=70736784

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2020/050477 WO2020188181A1 (fr) 2019-03-20 2020-03-09 Nouvelles souches de bacteries lactiques favorisant l'absorption du calcium - peptides et produits associes

Country Status (5)

Country Link
US (1) US20230287331A1 (fr)
EP (1) EP3942016A1 (fr)
KR (1) KR20210142692A (fr)
AU (1) AU2020243322A1 (fr)
WO (1) WO2020188181A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3102774B1 (fr) * 2019-10-31 2021-10-01 Vf Bioscience Nouvelle souche de lactobacillus plantarum utile pour l’absorption intestinale du calcium
CN115624175A (zh) * 2022-11-04 2023-01-20 大连澎立生物科技有限公司 一种富硒灵芝酵素小分子肽钙及其制备方法和富硒小分子钙多元蛋白肽

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3488722B2 (ja) 1992-03-04 2004-01-19 カルピス株式会社 カルシウム吸収促進活性剤及びその製造法
EP1820849B1 (fr) 2006-02-20 2009-01-21 Compagnie Gervais Danone Nouvelles souches de Lactobacillus helveticus
WO2008002484A2 (fr) 2006-06-23 2008-01-03 Lacpro Industries, Llc Nouvelle souche de lactobacillus bulgaricus et compositions la contenant
JP5686680B2 (ja) 2011-06-24 2015-03-18 カルピス株式会社 乳酸菌発酵によるカゼイン由来ペプチドの製造方法

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Publication number Publication date
AU2020243322A1 (en) 2021-10-28
US20230287331A1 (en) 2023-09-14
EP3942016A1 (fr) 2022-01-26
WO2020188181A1 (fr) 2020-09-24
KR20210142692A (ko) 2021-11-25

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