WO2020186601A1 - 一种检测前处理设备 - Google Patents

一种检测前处理设备 Download PDF

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Publication number
WO2020186601A1
WO2020186601A1 PCT/CN2019/084448 CN2019084448W WO2020186601A1 WO 2020186601 A1 WO2020186601 A1 WO 2020186601A1 CN 2019084448 W CN2019084448 W CN 2019084448W WO 2020186601 A1 WO2020186601 A1 WO 2020186601A1
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WIPO (PCT)
Prior art keywords
moving mechanism
axis moving
magnetic
liquid
axis
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PCT/CN2019/084448
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English (en)
French (fr)
Inventor
严义勇
刘自天
邓炀
彭明珠
付辉
朱海
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深圳市易瑞生物技术股份有限公司
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Publication of WO2020186601A1 publication Critical patent/WO2020186601A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples

Definitions

  • the invention relates to the technical field of biochemical detection, in particular to a detection pre-processing equipment.
  • the present invention provides a detection pre-processing device.
  • a pre-processing equipment for detection including:
  • the kit is arranged on the base, and the kit is provided with an extraction liquid warehouse, a magnetic bead warehouse and an elution warehouse;
  • Concentration device for concentrating the liquid to be concentrated in the kit
  • a liquid adding device for adding a reconstituted solution to the liquid processed by the concentration device
  • a magnetic separation device for adsorbing magnetic beads from the magnetic bead bin, adsorbing target compounds with the magnetic beads, and eluting the magnetic beads;
  • the mobile device is used to adjust the relative positions of the concentration device, the sample adding device, the magnetic separation device and the reagent kit;
  • the control device is respectively connected with the concentration device, the liquid adding device, the magnetic separation device, and the moving device.
  • the concentrating device includes a gas guide tube and an air pump, the air pump is in communication with the gas guide tube, the air pump is externally connected with gas, and the gas guide tube is provided with a gas flow valve for adjusting the gas flow , The gas flow valve is connected with the control device.
  • the air pump is a positive and negative pressure air pump
  • the positive and negative pressure air pump is connected to a valve
  • the valve is connected to the control device for controlling blowing or pumping of the positive and negative pressure air pump
  • the device also includes a detachable seal, and the gas guide tube is in sealed communication with the reagent box through the seal.
  • a heating device for heating the reagent box is provided on the base, and the heating device is connected with the control device.
  • the liquid adding device includes a liquid guide tube, a liquid pump is provided on the liquid guide tube, and the liquid pump is connected to the control device.
  • the magnetic separation device includes a magnet bar, a magnet sleeve, and a driving part, the magnet bar is slidably arranged in the magnet sleeve, and the driving part is drivingly connected to the magnet bar for driving the magnet bar in the The magnetic sleeve moves up and down.
  • the moving device includes a Y-axis moving mechanism and a Z-axis moving mechanism; the base is arranged on the Y-axis moving mechanism, and the Y-axis moving mechanism is used to drive the base to move in the Y-axis direction;
  • the Z-axis moving mechanism includes a first Z-axis moving mechanism, a second Z-axis moving mechanism, and a third Z-axis moving mechanism.
  • the first Z-axis moving mechanism is used to drive the concentrating device to move up and down in the Z-axis direction
  • the second Z-axis moving mechanism is used to drive the liquid adding device to move up and down in the Z-axis direction
  • the third Z-axis moving mechanism is used to drive the magnetic separation device to move up and down in the Z-axis direction.
  • the moving device further includes an X-axis moving mechanism, the Y-axis moving mechanism is arranged on the X-axis moving mechanism, and the X-axis moving mechanism is used to drive the Y-axis moving mechanism in the X-axis direction. mobile.
  • the moving device further includes an X-axis moving mechanism, the Z-axis moving mechanism is disposed on the X-axis moving mechanism, and the X-axis moving mechanism is used to drive the Z-axis moving mechanism to move in the X-axis direction. .
  • it also includes a cleaning device for cleaning the gas draft tube and the liquid draft tube, and the cleaning device is arranged on the moving track of the gas draft tube and the liquid draft tube.
  • the extraction solution can be concentrated by solvent volatilization, and the target compound in the extraction solution can be separated and purified by the magnetic separation device using magnetic beads, and it has the functions of concentration and separation and purification.
  • the entire process is automated by equipment , The operation is convenient, the man-made operation error is reduced, the labor intensity is reduced, the pre-processing efficiency is improved, and the physical injury of the organic reagent to the operator is reduced.
  • Figure 1 is a perspective view of the present invention
  • Figure 2 is another perspective view of the present invention.
  • orientation or positional relationship indicated by the terms “upper”, “lower”, “inner”, “outer”, etc. are based on the orientation or positional relationship shown in the drawings, or the The orientation or positional relationship usually placed in the use of the invention product is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, therefore It cannot be understood as a limitation to the present invention.
  • the terms “first”, “second”, “third”, etc. are only used for distinguishing description, and cannot be understood as indicating or implying relative importance.
  • connection should be interpreted broadly, for example, it may be a fixed connection or a detachable connection, or Integrally connected; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the internal communication between two components.
  • set and “connection” should be interpreted broadly, for example, it may be a fixed connection or a detachable connection, or Integrally connected; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the internal communication between two components.
  • a detection pre-processing equipment includes a base, a reagent box, a concentration device, a liquid adding device, a magnetic separation device, a moving device, and a control device.
  • the control device is respectively connected to the concentration device, the liquid adding device, the magnetic separation device, and the moving device. Device connection.
  • the reagent kit is arranged on the base, and the reagent kit is provided with an extraction liquid warehouse, a magnetic bead warehouse and an elution warehouse.
  • the extraction solution bin is used to place the extraction solution
  • the magnetic bead bin is used to place magnetic beads
  • the elution bin is used to elute the reaction conjugate.
  • the kit can also be provided with a cleaning chamber for storing cleaning liquid.
  • the concentration device is used to concentrate the liquid to be concentrated in the kit.
  • the concentrating device includes a gas guide tube and an air pump, the air pump is in communication with the gas guide tube, the gas pump is externally connected with gas, and the gas guide tube is provided with a gas flow valve for adjusting the gas flow , The gas flow valve is connected with the control device.
  • the air pump is a positive and negative pressure air pump
  • the positive and negative pressure air pump is connected to a valve
  • the valve is connected to the control device for controlling blowing or pumping of the positive and negative pressure air pump
  • the concentrating device also includes a detachable seal, and the gas guide tube is in sealed communication with the reagent box through the seal.
  • the air pump is externally connected with gas, and the gas is preferably but not limited to air.
  • Sample concentration is an important and critical step in the process of sample purification and preparation.
  • the most common concentration devices in laboratories mainly include rotary evaporators and nitrogen blowing devices.
  • the rotary evaporator rotates the solution that needs to be concentrated to increase the surface area of the solution and improve the efficiency of the evaporation method, but the rotary evaporator is suitable for the concentration of a single sample with a larger volume. When the volume is concentrated to a smaller volume, the concentration efficiency will become Very low.
  • the nitrogen blowing instrument mainly uses high-purity nitrogen to purge the surface of the solution, increase the gas flow rate on the surface of the solution, and blow away the solvent to increase the concentration of the solution. The advantage of the nitrogen blowing instrument is that it can concentrate multiple samples at a time and is easy to operate.
  • the disadvantage is that high-purity nitrogen is required for purging, which wastes high-purity nitrogen.
  • the concentration device of the present invention can quickly concentrate the liquid to be concentrated, is easy to operate, can concentrate multiple samples at a time, can use an air source, does not need to consume high-purity nitrogen, has a simple structure, and can greatly reduce the use cost.
  • the concentrating device of the present invention can be carried out in two ways, air blowing and air extraction, with wide user selectivity and great flexibility.
  • the concentrating device of the present invention is carried out by blowing air.
  • the air is pressurized by an air pump to form a high-speed airflow.
  • the high-speed airflow is distributed to the gas guide tube.
  • the gas guide tube affects the liquid to be concentrated in the kit. Purging is performed to quickly volatilize the solvent in the liquid to be concentrated, reducing concentration time;
  • the concentration device of the present invention adopts an air extraction method, and the gas guide tube and the reagent box are realized through a seal.
  • the air pump is in a state of negative pressure inside the kit, and the boiling point of the concentrated liquid is lowered.
  • the heating device is used to raise the temperature of the concentrated liquid to the boiling point of the solvent to accelerate the volatilization of the solvent.
  • the gas flow valve of the present invention is provided with a gas flow valve, which can independently control the opening of each gas flow tube and adjust the gas flow. If no extraction liquid is placed in the extraction liquid bin, the corresponding gas flow tube can be closed. save costs.
  • the base is also provided with a heating device for heating the reagent box, and the heating device is connected with the control device.
  • a heating device is provided to heat the kit, so that the sample can be processed according to the required temperature when the extract is concentrated.
  • the temperature can be set at 4-100°C in advance, and the heating device Adjust the temperature to the preset temperature to improve the efficiency of concentration.
  • the specific structure of the heating device is not specifically limited, and conventional settings in the field can be used as long as the heating effect is achieved.
  • the heating device can be heated by electric heating wire, electric film heating or water bath heating.
  • the liquid adding device is used for adding a compound solution to the liquid processed by the concentration device.
  • the present invention does not specifically limit the specific structure of the liquid addition device, which is a component known to those skilled in the art, and its structure and principles are all known to those skilled in the art through technical manuals or through conventional experimental methods.
  • the liquid adding device includes a liquid guide tube, a liquid pump is arranged on the liquid guide tube, and the liquid pump is connected to the control device.
  • the magnetic separation device is used for adsorbing magnetic beads from the magnetic bead bin, using the magnetic beads to adsorb the target compound in the extraction solution, and eluting the magnetic beads.
  • the magnetic beads may be immunomagnetic beads. More preferably, the magnetic beads are chemical magnetic beads, and the magnetic beads have a core-shell structure.
  • the core of the magnetic beads is magnetic, so that the magnetic beads can be Rod adsorption;
  • the shell surface of the magnetic beads has different functional groups, using different chemical functional groups (carboxyl, amino, hydroxyl, aromatic ring, etc.) and their combinations to form hydrogen bonds, ion pairs, and ⁇ - ⁇ interactions with the target compound. Bond to achieve selective adsorption of target compounds.
  • the magnetic rod is preferably an electromagnetic rod.
  • the magnetic separation device is adopted in the present invention, which can realize automation and mass operation; simple operation and short time; and can realize the purpose of extracting target compounds with high purity and high efficiency.
  • the magnetic separation device includes a magnet bar, a magnet sleeve, and a driving part, the magnet bar is slidably arranged in the magnet sleeve, and the driving part is drivingly connected to the magnet bar for driving the magnet bar at The magnetic sleeve moves up and down.
  • the magnetic rod is moved up and down by the driving part, the magnetic rod moves down into the magnetic sleeve, and moves upward to extend the magnetic sleeve.
  • the magnetic separation device includes a magnetic rod, a magnetic rod rack, a magnetic sleeve, a magnetic sleeve rack, and a driving part.
  • the magnetic sleeve rack is provided with a plurality of vertically arranged magnets that can extend into the reagent box.
  • a magnetic rod holder is arranged above the magnetic housing frame, and a plurality of magnetic rods that can extend into the magnetic housing are arranged on the magnetic rod frame, and the driving part is drivingly connected to the magnetic rod holder for The magnetic rod is driven to move up and down in the magnetic sleeve.
  • the driving part is used to realize the up and down movement of the magnet bar. It is a component known to those skilled in the art, and its structure and principle are all known to those skilled in the technical manual or through Known by conventional experimental methods.
  • the driving part includes a motor, a screw rod and a nut, the output shaft of the motor is drivingly connected to the screw rod, the nut is sleeved outside the screw rod, and the magnetic rod holder is fixedly connected to the Nut.
  • the concentration device, the liquid adding device, and the magnetic separation device are all arranged above the kit.
  • the control device controls the operation of the concentration device, the liquid adding device, the magnetic separation device, and the moving device.
  • the control device is a component known to those skilled in the art, and its structure and principle are all known to those skilled in the art through technical manuals or through conventional experimental methods. Those skilled in the art can set up according to actual needs.
  • the specific structure of the control device is not limited.
  • reagent kit there are more than one reagent kit, gas guide tube, liquid guide tube, magnetic rod, and magnetic sleeve, which can realize multi-channel concentration and purification.
  • the present invention also includes a cleaning device for cleaning the gas guide tube and the liquid guide tube, and the cleaning device is arranged on the moving track of the gas guide tube and the liquid guide tube.
  • the specific structure of the cleaning device is not specifically limited, and conventional settings in the field can be adopted.
  • the mobile device is used to adjust the relative positions of the concentration device, the sample adding device, the magnetic separation device and the reagent kit.
  • the moving device includes an X-axis moving mechanism, a Y-axis moving mechanism, and a Z-axis moving mechanism.
  • the Z-axis moving mechanism includes a first Z-axis moving mechanism, a second Z-axis moving mechanism, and a third Z-axis moving mechanism.
  • the first Z-axis moving mechanism is used to drive the concentrating device to move up and down in the Z-axis direction
  • the second Z-axis moving mechanism is used to drive the liquid adding device to move up and down in the Z-axis direction
  • the third Z-axis moving mechanism is used to drive the magnetic separation device to move up and down in the Z-axis direction.
  • the position of the gas guide tube in the concentrating device is adjusted up and down through the first Z-axis moving mechanism, which improves the concentration efficiency and makes the experiment operation more convenient.
  • the position of the liquid guide tube in the liquid adding device is adjusted up and down through the second Z-axis moving mechanism to prevent splashing of the compound solution and avoid cross contamination.
  • the position of the magnetic sleeve in the magnetic separation device is adjusted up and down through the third Z-axis moving mechanism to realize the adsorption and separation of the magnetic beads.
  • the base is arranged on the Y-axis moving mechanism, and the Y-axis moving mechanism is used to drive the base to move in the Y-axis direction.
  • the X-axis moving mechanism can be used to drive the reagent cartridge to move in the X-axis direction.
  • the base is arranged on the Y-axis moving mechanism
  • the Y-axis moving mechanism is arranged on the X-axis moving mechanism
  • the X-axis moving mechanism is used to drive the Y-axis movement The mechanism moves in the X-axis direction.
  • the X-axis moving mechanism can be used to drive the concentration device, the liquid adding device and the magnetic separation device to move in the X-axis direction.
  • the base is arranged on the Y-axis moving mechanism, and the first Z-axis moving mechanism, the second Z-axis moving mechanism, and the third Z-axis moving mechanism are all arranged on the base, so
  • the seat body is provided on the X-axis moving mechanism, and the X-axis moving mechanism is used to drive the seat body to move in the X-axis direction.
  • the direction along the z axis is defined as the up and down direction
  • the direction along the y axis is defined as the front and rear direction
  • the direction along the x axis is defined as the left and right direction.
  • the specific structures of the X-axis moving mechanism, Y-axis moving mechanism, and Z-axis moving mechanism are not particularly limited. Those skilled in the art can fully understand the X-axis moving mechanism, Y-axis moving mechanism, and Z-axis moving mechanism.
  • the shaft movement mechanism is used to realize lifting and horizontal movement, and it is a component known to those skilled in the art, and its structure and principle are all known to those skilled in the technical manual or through conventional experimental methods.
  • the X-axis moving mechanism and the Z-axis moving mechanism both use a linear guide, a sliding seat, a screw and a linear motor coordinated guide mechanism
  • the Y-axis moving mechanism uses a linear guide and a sliding seat coordinated guide mechanism.
  • the X-axis moving mechanism includes a base and a linear motor and a linear guide mounted on the base.
  • the linear guide is provided with a sliding seat
  • the linear motor is connected to the sliding seat through a screw
  • the linear motor drives the sliding through the screw.
  • the seat moves linearly along the linear guide.
  • the working process of the present invention is as follows: the Y-axis moving mechanism slides, so that the kit set on the Y-axis moving mechanism is pulled out, the extraction solution is added to the extraction solution bin of the kit, the magnetic beads are added to the magnetic bead bin, and the elution bin is Add the eluent; drive the magnetic rod into the magnetic sleeve through the drive part; heat the extracting solution tank to a preset temperature by the heating device; the X-axis moving mechanism slides so that the gas flow tube of the concentration device is aligned with the extracting solution tank; A Z-axis moving mechanism slides down, so that the gas guiding tube set on the first Z-axis moving mechanism descends to a position 1.5 cm away from the extraction liquid, and starts blowing; after the extraction liquid is blown dry, the X-axis moving mechanism Sliding so that the liquid guide tube in the liquid adding device is aligned with the extraction liquid chamber; the second Z-axis moving mechanism slides downward, so that the liquid guide tube arranged on the
  • the third Z-axis moving mechanism slides up and leaves the magnetic bead compartment; the X-axis moving mechanism slides to make the magnetic bars in the magnetic separation device align Extraction liquid silo; the third Z-axis moving mechanism slides downwards, so that the magnetic sleeve set on the third Z-axis moving mechanism goes down to the preset position for adsorption of the target compound; after the adsorption is completed, the third Z-axis moving mechanism moves upward Sliding away from the extraction fluid compartment; the X-axis moving mechanism slides so that the magnetic rod in the magnetic separation device is aligned with the elution compartment; the third Z-axis moving mechanism slides down to make the magnetic sleeve set on the third Z-axis moving mechanism Down to the preset position, the eluent is used to elute the target compound, and the elution is completed to obtain the test solution.
  • the working process of the present invention can also be as follows: the Y-axis moving mechanism slides, so that the kit set on the Y-axis moving mechanism is pulled out, the extraction solution is added to the extraction solution compartment of the kit, and the magnetic beads are added to the magnetic bead compartment to wash The eluent is added to the unloading bin, and the cleaning solution is added to the cleaning bin; the drive unit drives the magnetic rod to be inserted into the magnetic sleeve; the X-axis moving mechanism slides so that the magnetic rod in the magnetic separation device is aligned with the magnetic bead bin; third The Z-axis moving mechanism slides down to make the magnetic sleeve set on the third Z-axis moving mechanism go down to the preset position.
  • the third Z-axis moving mechanism slides up and leaves the magnetic bead compartment; X-axis moving mechanism Slide to make the magnetic rod in the magnetic separation device align with the extraction liquid chamber; the third Z-axis moving mechanism slides down, so that the magnetic sleeve set on the third Z-axis moving mechanism descends to the preset position to adsorb the target compound ; After the adsorption is completed, the third Z-axis moving mechanism slides upwards and leaves the extraction liquid warehouse; the X-axis moving mechanism slides so that the magnetic bar in the magnetic separation device is aligned with the cleaning chamber; the third Z-axis moving mechanism slides down to make The magnetic sleeve arranged on the third Z-axis moving mechanism moves down to the preset position to clean the magnetic beads; after the cleaning is completed, the third Z-axis moving mechanism slides up and leaves the cleaning chamber; the X-axis moving mechanism slides to separate the magnetic The magnetic rod in the device is aligned with the elution chamber; the third Z-axis moving mechanism slides down, so

Abstract

一种检测前处理设备，包括：底座（1）；试剂盒（2），设置于底座（1）上，试剂盒（2）上设有提取液仓、磁珠仓和洗脱仓；浓缩装置（3）；加液装置（4），用于加入复溶液；磁分离装置（5），用于从磁珠仓中吸附磁珠，用磁珠吸附目标化合物，并对磁珠进行洗脱；移动装置（6,7,8,9,10），用于调整浓缩装置（3）、加液装置（4）、磁分离装置（5）同试剂盒（2）之间的相对位置；及控制装置，控制装置分别与浓缩装置（3）、加液装置（4）、磁分离装置（8）、移动装置（6,7,8,9,10）连接。提取液可以通过溶剂挥发的方式实现浓缩，并且可以通过磁分离装置（5）利用磁珠对提取液中的目标化合物进行分离纯化，同时具有浓缩和分离纯化的功能，整个过程通过检测前处理设备实现自动化，操作方便。

Description

一种检测前处理设备 技术领域
本发明涉及生物化学检测技术领域，尤其涉及一种检测前处理设备。
背景技术
在小分子检测中，为了提高检测的特异性和灵敏度，通常需要对提取液进行浓缩和纯化。常见的浓缩手段有旋转蒸发、空气吹扫，常见的纯化手段有基于免疫法或化学亲和法的固相萃取柱。这些过程均为独立的步骤，不但占用了大量的实验室面积，更需要较为繁琐的人工操作，导致在检测过程中，需要耗费的时间较长，劳动强度大，人为操作导致的误差和结果重现性也较差。在使用过程中，有机试剂挥发的蒸汽对人员的伤害也较大。
发明概述
技术问题
问题的解决方案
技术解决方案
为了解决上述现有技术的不足，本发明提供一种检测前处理设备。
本发明所要解决的技术问题通过以下技术方案予以实现：
一种检测前处理设备，包括：
底座；
试剂盒，设置于所述底座上，所述试剂盒上设有提取液仓、磁珠仓和洗脱仓；
浓缩装置，用于对所述试剂盒内的待浓缩液体进行浓缩；
加液装置，用于对所述浓缩装置处理过的液体加入复溶液；
磁分离装置，用于从所述磁珠仓中吸附磁珠，用所述磁珠吸附目标化合物，并对所述磁珠进行洗脱；
移动装置，用于调整所述浓缩装置、加样装置、磁分离装置同所述试剂盒之间的相对位置；及
控制装置，所述控制装置分别与所述浓缩装置、加液装置、磁分离装置、移动 装置连接。
进一步地，所述浓缩装置包括气体导流管和气泵，所述气泵与所述气体导流管连通，所述气泵外接气体，所述气体导流管上设有用于调节气体流量的气体流量阀，所述气体流量阀与所述控制装置连接。
进一步地，所述气泵为正负压气泵，所述正负压气泵与阀门连接，所述阀门与所述控制装置连接，用于控制所述正负压气泵吹气或抽气；所述浓缩装置还包括可拆卸的密封件，所述气体导流管通过所述密封件与所述试剂盒密封连通。
进一步地，所述底座上设有用于对试剂盒进行加热的加热装置，所述加热装置与所述控制装置连接。
进一步地，所述加液装置包括液体导流管，所述液体导流管上设有液体泵，所述液体泵与所述控制装置连接。
进一步地，所述磁分离装置包括磁棒、磁套和驱动部，所述磁棒滑动设置于所述磁套内，所述驱动部驱动连接于所述磁棒用于驱动所述磁棒在所述磁套内上下运动。
进一步地，所述移动装置包括Y轴移动机构和Z轴移动机构；所述底座设置在所述Y轴移动机构上，所述Y轴移动机构用于驱动所述底座在Y轴方向上移动；所述Z轴移动机构包括第一Z轴移动机构、第二Z轴移动机构和第三Z轴移动机构，所述第一Z轴移动机构用于驱动所述浓缩装置在Z轴方向上下运动，所述第二Z轴移动机构用于驱动所述加液装置在Z轴方向上下运动，所述第三Z轴移动机构用于驱动所述磁分离装置在Z轴方向上下运动。
进一步地，所述移动装置还包括X轴移动机构，所述Y轴移动机构设置在所述X轴移动机构上，所述X轴移动机构用于驱动所述Y轴移动机构在X轴方向上移动。
进一步地，所述移动装置还包括X轴移动机构，所述Z轴移动机构设置在所述X轴移动机构，所述X轴移动机构用于驱动所述Z轴移动机构在X轴方向上移动。
进一步地，还包括用于清洗所述气体导流管和液体导流管的清洗装置，所述清洗装置设置于所述气体导流管和液体导流管的移动轨迹上。
发明的有益效果
有益效果
本发明中，提取液可以通过溶剂挥发的方式实现浓缩，并且可以通过磁分离装置利用磁珠对提取液中的目标化合物进行分离纯化，同时具有浓缩和分离纯化的功能，整个过程通过设备实现自动化，操作方便，减少了人为的操作误差，降低劳动强度，提高了前处理效率，减少有机试剂对操作人员的身体伤害。
对附图的简要说明
附图说明
图1为本发明的立体图；
图2为本发明的另一立体图。
图中：1、底座，2、试剂盒，3、浓缩装置，4、加液装置，5、磁分离装置，6、X轴移动机构，7、Y轴移动机构，8、第一Z轴移动机构，9、第二Z轴移动机构，10、第三Z轴移动机构，11、清洗装置。
发明实施例
本发明的实施方式
为使本发明实施例的目的、技术方案和优点更加清楚，下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例是本发明一部分实施例，而不是全部的实施例。通常在此处附图中描述和示出的本发明实施例的组件可以以各种不同的配置来布置和设计。
因此，以下对在附图中提供的本发明的实施例的详细描述并非旨在限制要求保护的本发明的范围，而是仅仅表示本发明的选定实施例。基于本发明中的实施例，本领域普通技术人员在没有作出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
在本发明的描述中，需要说明的是，术语“上”、“下”、“内”、“外”等指示的方位或位置关系为基于附图所示的方位或位置关系，或者是该发明产品使用时惯常摆放的方位或位置关系，仅是为了便于描述本发明和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本发明的限制。此外，术语“第一”、“第二”、“第三”等仅用于区分描述，而不能理解为指示或暗示相对重要性。
在本发明的描述中，还需要说明的是，除非另有明确的规定和限定，术语“设置”、“连接”应做广义理解，例如，可以是固定连接，也可以是可拆卸连接，或一体地连接；可以是机械连接，也可以是电连接；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通。对于本领域的普通技术人员而言，可以具体情况理解上述术语在本发明中的具体含义。
一种检测前处理设备，包括底座、试剂盒、浓缩装置、加液装置、磁分离装置、移动装置和控制装置，所述控制装置分别与所述浓缩装置、加液装置、磁分离装置、移动装置连接。
所述试剂盒设置于所述底座上，所述试剂盒上设有提取液仓、磁珠仓和洗脱仓。所述提取液仓用于放置提取液，所述磁珠仓用于放置磁珠，所述洗脱仓用于洗脱反应结合物。
所述试剂盒上还可以设置清洗仓，用于放置清洗液。
所述浓缩装置用于对所述试剂盒内的待浓缩液体进行浓缩。
具体地，所述浓缩装置包括气体导流管和气泵，所述气泵与所述气体导流管连通，所述气泵外接气体，所述气体导流管上设有用于调节气体流量的气体流量阀，所述气体流量阀与所述控制装置连接。
作为进一步改进，所述气泵为正负压气泵，所述正负压气泵与阀门连接，所述阀门与所述控制装置连接，用于控制所述正负压气泵吹气或抽气；所述浓缩装置还包括可拆卸的密封件，所述气体导流管通过所述密封件与所述试剂盒密封连通。
本发明中，所述气泵外接气体，所述气体优选但不限定为空气。
样品浓缩是样品的纯化和制备过程中比较重要而关键的一个步骤，实验室最常见的浓缩装置主要有旋转蒸发仪、氮吹仪。旋转蒸发仪是对需要浓缩的溶液进行旋转，使得溶液表面积增大，提高蒸法效率，但旋转蒸发仪适用于体积较大的单样品浓缩，当浓缩至体积较小时，浓缩效率就会变得很低。氮吹仪主要是利用高纯氮气吹扫溶液表面，增加溶液表面气体流通速度，将溶剂吹走使溶液浓度增大。氮吹仪的优点是可以一次浓缩多个样品，操作简便。缺点是需要高纯氮气吹扫，浪费高纯氮气。本发明的浓缩装置可以迅速浓缩待浓缩液体，操 作简便，可以一次浓缩多个样品，而且可以使用空气源，无需消耗高纯氮气，结构简单，可以大大降低使用成本。
本发明的浓缩装置可采用吹气、抽气两种方式进行，用户选择性广、灵活性大。对于不敏感的目标物，本发明的浓缩装置采用吹气方式进行，空气经过气泵加压，形成高速气流，高速气流分配到气体导流管中，气体导流管对试剂盒中的待浓缩液体进行吹扫，使待浓缩液体中的溶剂快速挥发，减少浓缩时间；对于敏感的目标物，本发明的浓缩装置采用抽气方式进行，通过密封件实现气体导流管与所述试剂盒之间的密封连通，气泵抽气，使得试剂盒内处于负压状态，待浓缩液的沸点降低，通过加热装置使待浓缩液升温达到溶剂沸点，加速溶剂的挥发。
本发明的气体导流管上设置有气体流量阀，可以独立控制每个气体导流管的开启并调节气体流量，若提取液仓中没有放置提取液，则可关闭对应的气体导流管，节约成本。
所述底座上还设有用于对试剂盒进行加热的加热装置，所述加热装置与所述控制装置连接。本发明中，通过设置加热装置，用于对所述试剂盒进行加热，这样，样品处理前就能根据提取液浓缩时所需温度，可以预先在4-100℃设置温度，通过所述加热装置调节温度至预设的温度，提高浓缩的效率。
本发明中，并不具体限定加热装置的具体结构，可以采用本领域的常规设置，只要实现加热效果即可，作为举例，所述加热装置可以采用电加热丝加热、电热膜加热或水浴加热。
所述加液装置用于对所述浓缩装置处理过的液体加入复溶液。本发明对加液装置的具体结构不作特别限定，其为本领域技术人员知晓的部件，其结构和原理都为本技术人员均可通过技术手册得知或通过常规实验方法获知。作为优选，所述加液装置包括液体导流管，所述液体导流管上设有液体泵，所述液体泵与所述控制装置连接。
所述磁分离装置用于从所述磁珠仓中吸附磁珠，用所述磁珠吸附提取液中的目标化合物，并对所述磁珠进行洗脱。
本发明中，所述磁珠可以为免疫磁珠，更优选地，所述磁珠为化学磁珠，所述 磁珠为核壳结构，其中磁珠的内核具有磁性，使得磁珠能够被磁棒吸附；磁珠的外壳表面具有不同官能团，利用不同化学官能团(羧基、氨基、羟基、芳香环等)及其组合，与目标化合物形成氢键、离子对，π-π相互作用等非共价键，实现对目标化合物的选择性吸附。
所述磁棒优选为电磁棒。
本发明中采用磁分离装置，能够实现自动化、大批量操作；操作简单、用时短；可以实现高纯度、高效率提取目标化合物的目的。
具体地，所述磁分离装置包括磁棒、磁套和驱动部，所述磁棒滑动设置于所述磁套内，所述驱动部驱动连接于所述磁棒用于驱动所述磁棒在所述磁套内上下运动。
本发明中，通过驱动部使磁棒上下运动，磁棒向下运动到磁套内，向上运动伸出磁套。
更具体地，所述磁分离装置包括磁棒、磁棒架、磁套、磁套架和驱动部，所述磁套架上设有若干竖直布置且能伸入所述试剂盒内的磁套，所述磁套架的上方设有磁棒架，所述磁棒架上设有若干能伸入所述磁套内的磁棒，所述驱动部驱动连接于所述磁棒架用于驱动所述磁棒在所述磁套内上下运动。
对于本领域技术人员来说，完全能够理解驱动件用于实现磁棒的上下运动，其是本领域技术人员知晓的部件，其结构和原理都为本技术人员均可通过技术手册得知或通过常规实验方法获知。作为优选，所述驱动部包括电机、丝杆和螺母，所述电机的输出轴驱动连接所述丝杆，所述螺母套设于所述丝杆外，所述磁棒架固定连接于所述螺母。
优选地，所述浓缩装置、加液装置、和磁分离装置均设置于所述试剂盒的上方。
所述控制装置控制所述浓缩装置、加液装置、磁分离装置、移动装置的运行。所述控制装置为本领域技术人员知晓的部件，其结构和原理都为本技术人员均可通过技术手册得知或通过常规实验方法获知，本领域技术人员可以根据实际需求进行设置，本发明对控制装置的具体结构不作限定。
本发明中，所述的试剂盒、气体导流管、液体导流管、磁棒、磁套均为一个以 上，可以实现多通道浓缩和纯化。
作为进一步改进，本发明还包括用于清洗所述气体导流管和液体导流管的清洗装置，所述清洗装置设置于所述气体导流管和液体导流管的移动轨迹上。
本发明中，对于清洗装置的具体结构不作具体限定，可以采用本领域的常规设置。
所述移动装置用于调整所述浓缩装置、加样装置、磁分离装置同所述试剂盒之间的相对位置。
所述移动装置包括X轴移动机构、Y轴移动机构、和Z轴移动机构。
所述Z轴移动机构包括第一Z轴移动机构、第二Z轴移动机构和第三Z轴移动机构，所述第一Z轴移动机构用于驱动所述浓缩装置在Z轴方向上下运动，所述第二Z轴移动机构用于驱动所述加液装置在Z轴方向上下运动，所述第三Z轴移动机构用于驱动所述磁分离装置在Z轴方向上下运动。本发明中，通过第一Z轴移动机构对浓缩装置中气体导流管的位置进行上下调整，提高浓缩效率，使实验操作更加方便。本发明中，通过第二Z轴移动机构对加液装置中液体导流管的位置进行上下调整，防止复溶液飞溅，避免交叉污染。本发明中，通过第三Z轴移动机构对磁分离装置中磁套的位置进行上下调整，实现磁珠的吸附和分离。
所述底座设置在所述Y轴移动机构上，所述Y轴移动机构用于驱动所述底座在Y轴方向上移动。
本发明中，所述X轴移动机构可以用于驱动所述试剂盒在X轴方向上移动。
具体地，参阅图1，所述底座设置在所述Y轴移动机构上，所述Y轴移动机构设置在所述X轴移动机构上，所述X轴移动机构用于驱动所述Y轴移动机构在X轴方向上移动。
本发明中，所述X轴移动机构可以用于驱动所述浓缩装置、加液装置和磁分离装置在X轴方向上移动。
具体地，参阅图2，所述底座设置在所述Y轴移动机构上，所述第一Z轴移动机构、第二Z轴移动机构和第三Z轴移动机构都设置在一座体上，所述座体设置在所述X轴移动机构，所述X轴移动机构用于驱动所述座体在X轴方向上移动。
需要说明的是，定义沿z轴方向为上下方向，沿y轴方向为前后方向，沿x轴方 向为左右方向。
本发明中，关于X轴移动机构、Y轴移动机构、和Z轴移动机构的具体结构没有特别限定，对于本领域技术人员来说，完全能够理解X轴移动机构、Y轴移动机构、和Z轴移动机构用于实现升降和水平移动，其为本领域技术人员知晓的部件，其结构和原理都为本技术人员均可通过技术手册得知或通过常规实验方法获知。
作为举例，所述X轴移动机构和Z轴移动机构，均采用直线导轨、滑座、丝杆和直线电机相配合的导向机构，所述Y轴移动机构采用直线导轨和滑座相配合的导向机构。具体地，所述X轴移动机构包括基座以及安装在基座上的直线电机和直线导轨，直线导轨上设有滑座，直线电机通过丝杆与滑座连接，直线电机通过丝杆驱动滑座沿直线导轨进行直线运动。
本发明的工作过程为：Y轴移动机构滑动，使得设置于Y轴移动机构上的试剂盒拉出，向试剂盒的提取液仓中加入提取液，磁珠仓中加入磁珠，洗脱仓中加入洗脱液；通过驱动部驱动磁棒套入磁套；加热装置将提取液仓加热至预设温度；X轴移动机构滑动，使得浓缩装置的气体导流管对准提取液仓；第一Z轴移动机构向下滑动，使得设置于第一Z轴移动机构上的气体导流管下行至离提取液1.5cm的位置，开始吹气；待提取液被吹干后，X轴移动机构滑动，使得加液装置中的液体导流管对准所述提取液仓；第二Z轴移动机构向下滑动，使得设置于第二Z轴移动机构上的液体导流管下行至预设位置，液体导流管往提取液仓中加入复溶液；X轴移动机构滑动，使得磁分离装置中的磁棒对准所述磁珠仓；第三Z轴移动机构向下滑动，使得设置于第三Z轴移动机构上的磁套下行至预设位置，吸附磁珠后，第三Z轴移动机构向上滑动，离开磁珠仓；X轴移动机构滑动，使得磁分离装置中的磁棒对准提取液仓；第三Z轴移动机构向下滑动，使得设置于第三Z轴移动机构上的磁套下行至预设位置，进行目标化合物的吸附；吸附完成后，第三Z轴移动机构向上滑动，离开提取液仓；X轴移动机构滑动，使得磁分离装置中的磁棒对准洗脱仓；第三Z轴移动机构向下滑动，使得设置于第三Z轴移动机构上的磁套下行至预设位置，洗脱液进行目标化合物的洗脱，洗脱完成，得到待测液。
本发明的工作过程还可以为：Y轴移动机构滑动，使得设置于Y轴移动机构上的试剂盒拉出，向试剂盒的提取液仓中加入提取液，磁珠仓中加入磁珠，洗脱仓中加入洗脱液，清洗仓中加入清洗液；通过驱动部驱动磁棒套入磁套；X轴移动机构滑动，使得磁分离装置中的磁棒对准所述磁珠仓；第三Z轴移动机构向下滑动，使得设置于第三Z轴移动机构上的磁套下行至预设位置，吸附磁珠后，第三Z轴移动机构向上滑动，离开磁珠仓；X轴移动机构滑动，使得磁分离装置中的磁棒对准提取液仓；第三Z轴移动机构向下滑动，使得设置于第三Z轴移动机构上的磁套下行至预设位置，进行目标化合物的吸附；吸附完成后，第三Z轴移动机构向上滑动，离开提取液仓；X轴移动机构滑动，使得磁分离装置中的磁棒对准提清洗仓；第三Z轴移动机构向下滑动，使得设置于第三Z轴移动机构上的磁套下行至预设位置，进行磁珠的清洗；清洗完成后，第三Z轴移动机构向上滑动，离开清洗仓；X轴移动机构滑动，使得磁分离装置中的磁棒对准洗脱仓；第三Z轴移动机构向下滑动，使得设置于第三Z轴移动机构上的磁套下行至预设位置，洗脱液进行目标化合物的洗脱，洗脱完成后，第三Z轴移动机构向上滑动，离开洗脱仓。
以上所述实施例仅表达了本发明的实施方式，其描述较为具体和详细，但并不能因此而理解为对本发明专利范围的限制，但凡采用等同替换或等效变换的形式所获得的技术方案，均应落在本发明的保护范围之内。

Claims (10)

  1. 一种检测前处理设备，其特征在于，其包括：
    底座；
    试剂盒，设置于所述底座上，所述试剂盒上设有提取液仓、磁珠仓和洗脱仓；
    浓缩装置，用于对所述试剂盒内的待浓缩液体进行浓缩；
    加液装置，用于对所述浓缩装置处理过的液体加入复溶液；
    磁分离装置，用于从所述磁珠仓中吸附磁珠，用所述磁珠吸附目标化合物，并对所述磁珠进行洗脱；
    移动装置，用于调整所述浓缩装置、加样装置、磁分离装置同所述试剂盒之间的相对位置；及
    控制装置，所述控制装置分别与所述浓缩装置、加液装置、磁分离装置、移动装置连接。
  2. 如权利要求1所述的检测前处理设备，其特征在于，所述浓缩装置包括气体导流管和气泵，所述气泵与所述气体导流管连通，所述气泵外接气体，所述气体导流管上设有用于调节气体流量的气体流量阀，所述气体流量阀与所述控制装置连接。
  3. 如权利要求2所述的检测前处理设备，其特征在于，所述气泵为正负压气泵，所述正负压气泵与阀门连接，所述阀门与所述控制装置连接，用于控制所述正负压气泵吹气或抽气；所述浓缩装置还包括可拆卸的密封件，所述气体导流管通过所述密封件与所述试剂盒密封连通。
  4. 如权利要求1所述的检测前处理设备，其特征在于，所述底座上设有用于对试剂盒进行加热的加热装置，所述加热装置与所述控制装置连接。
  5. 如权利要求2所述的检测前处理设备，其特征在于，所述加液装置包括液体导流管，所述液体导流管上设有液体泵，所述液体泵与所述控制装置连接。
  6. 如权利要求1所述的检测前处理设备，其特征在于，所述磁分离装置包括磁棒、磁套和驱动部，所述磁棒滑动设置于所述磁套内，
    所述驱动部驱动连接于所述磁棒用于驱动所述磁棒在所述磁套内上下运动。
  7. 如权利要求1所述的检测前处理设备，其特征在于，所述移动装置包括Y轴移动机构和Z轴移动机构；所述底座设置在所述Y轴移动机构上，所述Y轴移动机构用于驱动所述底座在Y轴方向上移动；所述Z轴移动机构包括第一Z轴移动机构、第二Z轴移动机构和第三Z轴移动机构，所述第一Z轴移动机构用于驱动所述浓缩装置在Z轴方向上下运动，所述第二Z轴移动机构用于驱动所述加液装置在Z轴方向上下运动，所述第三Z轴移动机构用于驱动所述磁分离装置在Z轴方向上下运动。
  8. 如权利要求7所述的检测前处理设备，其特征在于，所述移动装置还包括X轴移动机构，所述Y轴移动机构设置在所述X轴移动机构上，所述X轴移动机构用于驱动所述Y轴移动机构在X轴方向上移动。
  9. 如权利要求7所述的检测前处理设备，其特征在于，所述移动装置还包括X轴移动机构，所述Z轴移动机构设置在所述X轴移动机构，所述X轴移动机构用于驱动所述Z轴移动机构在X轴方向上移动。
  10. 如权利要求5所述的检测前处理设备，其特征在于，还包括用于清洗所述气体导流管和液体导流管的清洗装置，所述清洗装置设置于所述气体导流管和液体导流管的移动轨迹上。
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