WO2020178951A1 - Dispositif de manipulation de fluide - Google Patents

Dispositif de manipulation de fluide Download PDF

Info

Publication number
WO2020178951A1
WO2020178951A1 PCT/JP2019/008412 JP2019008412W WO2020178951A1 WO 2020178951 A1 WO2020178951 A1 WO 2020178951A1 JP 2019008412 W JP2019008412 W JP 2019008412W WO 2020178951 A1 WO2020178951 A1 WO 2020178951A1
Authority
WO
WIPO (PCT)
Prior art keywords
region
chamber
handling device
fluid handling
opening
Prior art date
Application number
PCT/JP2019/008412
Other languages
English (en)
Inventor
Ashok Sinha
Ben Whiteley
Koichi Ono
Original Assignee
Enplas Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enplas Corporation filed Critical Enplas Corporation
Priority to PCT/JP2019/008412 priority Critical patent/WO2020178951A1/fr
Publication of WO2020178951A1 publication Critical patent/WO2020178951A1/fr

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Definitions

  • the present invention relates to a fluid handling device.
  • micro liquid droplets (hereinafter referred to also as ‘‘droplet’’) having a diameter of 0.1 to 1000 micrometre are generated from liquid containing the analysis object so as to observe and analyze the droplets.
  • the generated droplets are collected in a micro tube or the like. Then, typically, a required amount of the droplet is separated from the micro tube for an observation and/or analysis.
  • An apparatus provided with a needle is proposed as an apparatus for separation of the droplets collected in the micro tube or the like (e.g. PTL 1).
  • the droplets are typically dispersed in solvent.
  • the number of droplets to be housed is small.
  • the concentration of the droplets is increased before housing the droplets in the chip, the fluidity is reduced and the housing of the droplets becomes difficult.
  • the droplets are also discharged and/or clogging of the outlet with the droplets results. That is, in the conventional methods, it is difficult to house droplets having a high concentration in a chip.
  • an object of the present invention is to provide a fluid handling device that can house microparticles such as droplets while increasing the concentration of the microparticles.
  • a fluid handling device includes: a chamber including a first opening and a second opening in a wall surface; a first channel whose one end is connected with the first opening of the chamber; and a second channel whose one end is connected with the second opening of the chamber.
  • the chamber includes a first region and a second region, the first region being closer to the first opening than the second region, the second region being closer to the second opening than the first region.
  • a height of an interior of the chamber is smaller in the second region than in the first region.
  • a fluid handling device that can house microparticles such as droplets while increasing the concentration of the microparticles is provided.
  • FIG. 1A is a plan view illustrating a configuration of a fluid handling device according to Embodiment 1 of the present invention, and FIG. 1B is a sectional view of the fluid handling device illustrated in FIG. 1A along line A-A;
  • FIG. 2A is a bottom view of the fluid handling device illustrated in FIG. 1A, and
  • FIG. 2B is a plan view of a main body part of the fluid handling device illustrated in FIG. 1A;
  • FIG. 3A is a plan view of a main body part of a fluid handling device according to Embodiment 2 of the present invention, and FIG. 3B is a sectional view taken along line B-B of FIG. 3A;
  • FIG. 4A is a plan view of a main body part of a fluid handling device according to Embodiment 3 of the present invention
  • FIG. 4B is a partially enlarged perspective view of a boundary indicated with the broken line in FIG. 4A
  • FIG. 4C is a partially enlarged perspective view of the boundary in the state where the fluid handling device is used
  • FIGS. 5A to 5D are partially enlarged perspective views of a boundary of a fluid handling device according to a modification of Embodiment 3
  • FIG. 6A is a plan view illustrating a main body part of a fluid handling device according to a modification of the present invention
  • FIG. 6B is a plan view illustrating a main body part of a fluid handling device according to another modification of the present invention.
  • the fluid handling device of an embodiment of the present invention houses microparticles of fluid containing the microparticles and solvent while increasing the concentration of the microparticles.
  • the fluid handling device can be used for storing a large amount of microparticles and for observing microparticles.
  • microparticles means solid microparticles or liquid droplets (hereinafter referred to also as ‘‘droplets’’), and the type of microparticles is not limited.
  • the microparticles may have a microparticle size of 0.1 micrometre to 1000 micrometre. Preferably, the microparticle size is 5 micrometre to 200 micrometre.
  • the solid microparticles that can be housed in the fluid handling device of the present invention may be any microparticles of organic or inorganic materials.
  • the liquid droplets (droplets) contain various components (e.g. cells, DNAs, and proteins such as enzymes) and a dispersion solvent for dissolving and/or dispersing the components.
  • Each droplet may be a liquid droplet having a substantially spherical shape, and may be produced by a publicly known method.
  • the solvent for dispersing the above-mentioned microparticles is not limited.
  • the solvent may be appropriately selected in accordance with the type of microparticles, and in the case where the microparticles are droplets, it is possible to use a solvent (hereinafter referred to also as ‘‘host solvent’’) having a poor compatibility with the components and/or the dispersion solvent included in the droplet.
  • host solvent a solvent having a poor compatibility with the components and/or the dispersion solvent included in the droplet.
  • FIG. 1A is a plan view of fluid handling device 100 according to Embodiment 1 of the present invention
  • FIG. 1B is a sectional view taken along line A-A of FIG. 1A
  • FIG. 2A is a bottom view of fluid handling device 100
  • FIG. 2B is a plan view of main body part 101 of fluid handling device 100 in the state where cover 102 is dismounted.
  • fluid handling device 100 includes main body part 101 including a groove, a recess, a through hole and/or the like, and plate-shaped cover 102 that is bonded on the main body part 101 so as to cover the groove, the recess, the through hole and/or the like.
  • Fluid handling device 100 includes chamber 120 including first opening 122 and second opening 123 in the wall surface, first channel 110 connected with first opening 122 of the chamber 120, and second channel 130 connected with second opening 123 of chamber 120.
  • cover 102 is disposed on the upper side of main body part 101 in the gravity direction.
  • the orientation of fluid handling device 100 is not limited to the above-mentioned orientation.
  • main body part 101 may be disposed on the upper side of cover 102 in the gravity direction.
  • first opening 122 may be disposed on the upper side or the lower side with respect to second opening 123 in the gravity direction.
  • chamber 120 of the present embodiment includes a hollow part defined, in a substantially hexagonal prism shape, by the wall surface and the bottom surface of chamber recess 120a disposed in main body part 101, and cover 102.
  • first opening 122 and second opening 123 are provided in the upper portion of the wall of chamber recess 120a. Chamber 120 is communicated with first channel 110 described later through first opening 122, and is communicated with second channel 130 described later through second opening 123.
  • first opening 122 and second opening 123 are not limited, it is preferable that first opening 122 and second opening 123 be separated from each other in view of housing a large amount of microparticles.
  • the openings are disposed at opposite corners in chamber recess 120a having a hexagonal shape in plan view.
  • the shape of the hollow part of chamber 120 is not limited to the substantially hexagonal prism shape, and may be appropriately set in accordance with the use of fluid handling device 100 and/or the number of microparticles to be used.
  • the shape may be a substantially square columnar shape, a substantially columnar shape, a substantially ellipsoidal columnar shape or the like.
  • chamber 120 includes two regions differing in the height of the interior (the hollow part) thereof.
  • chamber 120 includes first region 121a located on first opening 122 side and second region 121b located on second opening 123 side, and second region 121b has a height smaller than that of the first region 121a.
  • the height of the interior of chamber 120 is the length from the bottom surface of chamber recess 120a to cover 102 in each region. Note that the area ratio of first region 121a and second region 121b in chamber 120 in plan view is not limited.
  • First region 121a need only be a region having height h1 larger than the diameter of the microparticles to be introduced into chamber 120 and can allow the fluid to freely flow. While height h1 of first region 121a is constant in the present embodiment, height h1 may vary from first opening 122 side to second region 121b side so that the flow of the fluid is facilitated. In addition, a groove, a guide and/or the like may be provided in a part of the region for the purpose of controlling the flow of the fluid.
  • second region 121b is a region having a height h2 smaller than the diameter of the microparticles to be introduced into chamber 120. Height h2 of second region 121b is not limited as long as only solvent can pass through second region 121b.
  • the boundary between first region 121a and second region 121b is formed in a linear shape. That is, the step surface between first region 121a and second region 121b is a flat surface.
  • the step surface serves as a partition wall for blocking microparticles introduced into chamber 120, and allows only the solvent included in the fluid to flow to second region 121b side. With this configuration, it is possible to suppress movement of microparticles to second opening 123 side through second region 121b. While the step surface is perpendicular to the bottom surface of first region 121a and the bottom surface of second region 121b in the present embodiment, the step surface may not be perpendicular to the bottom surface.
  • First channel 110 of fluid handling device 100 of the present embodiment is a channel whose one end is connected with first opening 122 of chamber 120, and the other end is connected with fluid inlet 111 and microparticle outlet 112.
  • first channel 110 is a region surrounded by first channel groove 110a disposed in main body part 101 and cover 102.
  • first channel 110 is divided into two channels such that one channel is connected with fluid inlet 111 for introducing fluid, and the other channel is connected with microparticle outlet 112 for removing microparticles as necessary.
  • first channel 110 may be connected with two or more fluid inlets 111.
  • first channel 110 is connected with a plurality of fluid inlets 111, different fluids can be introduced from respective fluid inlets 111, and the fluids thus introduced can be mixed in first channel 110, for example.
  • first channel 110 is not limited as long as the fluid can be kept flowing, and in the present embodiment, the depth of first channel 110 is substantially equal to height h1 of first region 121a of chamber 120. Also, the width thereof is not limited as long as the width is greater than the diameter of the microparticle.
  • Fluid inlet 111 and microparticle outlet 112 communicated with first channel 110 are through holes disposed in main body part 101, and their diameters are not limited as long as the fluid can be introduced into fluid handling device 100 and the microparticles can be output as necessary after microparticles are housed in chamber 120.
  • fluid inlet 111 and/or microparticle outlet 112 may be connected with a syringe, a tube and the like for introducing fluid.
  • second channel 130 is a channel whose one end is connected with second opening 123 of chamber 120 and the other end is connected with solvent outlet 131 for the fluid.
  • second channel 130 is a region surrounded by second channel groove 130a disposed in main body part 101 and cover 102.
  • the depth (height) of second channel 130 is not limited as long as the solvent can be discharged from solvent outlet 131 at a desired velocity. While the height of the second channel is substantially equal to height h2 of second region 121b of chamber 120 in the present embodiment, the second channel 130 may be higher or lower than second region 121b.
  • the width of second channel 130 is not limited, and may be appropriately set in accordance with the amount of the solvent to be discharged.
  • solvent outlet 131 communicated with second channel 130 is a through hole disposed in main body part 101, and the diameter thereof is not limited as long as the solvent can be discharged out of fluid handling device 100.
  • cover 102 of fluid handling device 100 may be a flat film or a plate-shaped member that covers the openings of chamber recess 120a, first channel groove 110a, second channel groove 130a, fluid inlet 111, microparticle outlet 112, solvent outlet 131 and the like of main body part 101.
  • the thickness and the like of the cover 102 may be appropriately set as long as the cover 102 is composed of a material that is not eroded by the fluid (in particular, the host solvent) introduced to fluid handling device 100.
  • the material of the cover may be an inorganic material such as glass; or a resin material such as polyester such as polyethylene terephthalate; polycarbonate; acrylic resin such as polymethylmethacrylate; polyvinyl chloride; polyolefin such as polyethylene, polypropylene, and cycloolefin resin; polyether; polystyrene; silicone resin; and elastomers.
  • a resin material such as polyester such as polyethylene terephthalate; polycarbonate; acrylic resin such as polymethylmethacrylate; polyvinyl chloride; polyolefin such as polyethylene, polypropylene, and cycloolefin resin; polyether; polystyrene; silicone resin; and elastomers.
  • cover 102 may be or may not be optically transparent.
  • cover 102 in the case where microparticles housed in chamber 120 are observed and/or analyzed from cover 102 side, it is preferable that cover 102 be optically transparent.
  • cover 102 need not have optical transparency.
  • Main body part 101 need only include the above-described fluid inlet 111, first channel groove 110a, chamber recess 120a, second channel groove 130a, and solvent outlet 131.
  • the material of main body part 101 include resin materials such as polyester such as polyethylene terephthalate; polycarbonate; acrylic resins such as polymethylmethacrylate; polyvinyl chloride; polyolefin such as polyethylene, polypropylene, and cycloolefin resin; polyether; polystyrene; silicone resins such as polydimethyl siloxane; and elastomers.
  • the main body part having the above-mentioned configuration may be formed by injection molding and the like, for example.
  • main body part 101 may be integrally formed, or may be composed of a combination of a plurality of members.
  • main body part 101 may be or may not be optically transparent.
  • the material of main body part 101 is selected such that main body part 101 is optically transparent.
  • main body part 101 and cover 102 may be joined by heat fusing, bonding with an adhesive agent and the like, and may be joined by publicly known methods.
  • Fluid Handling Method A fluid handling method using fluid handling device 100 is described below.
  • a microparticle manufacturing apparatus (not illustrated) or the like and fluid inlet 111 of fluid handling device 100 are connected with each other with a tube (not illustrated) or the like.
  • solvent outlet 131 of fluid handling device 100 and a container (not illustrated) or the like for collecting the solvent are connected with each other with a tube (not illustrated) or the like.
  • first channel 110, chamber 120, and second channel 130 are preliminarily filled with solvent that does not contain microparticles.
  • ‘‘solvent that does not contain microparticles’’ only be a solvent having poor compatibility with microparticles.
  • the solvent may be identical to or different from the above-described host solvent.
  • the fluid is introduced into chamber 120 through fluid inlet 111 and first channel 110.
  • a pressure application from fluid inlet 111 side and/or suction from solvent outlet 131 side may be performed to facilitate the flow of the fluid.
  • the fluid flowing into chamber 120 moves from first region 121a side toward second region 121b side.
  • the microparticles stay in first region 121a without moving between the bottom surface of second region 121b and cover 102.
  • the solvent in the fluid moves between the bottom surface of second region 121b and cover 102 toward second opening 123 side (second channel 130 side).
  • the solvent is discharged from solvent outlet 131, and the microparticles are predominantly housed in chamber 120 (at a position on first region 121a side).
  • microparticles housed in chamber 120 of fluid handling device 100 can be output from chamber 120 as necessary by suction from microparticle outlet 112, for example.
  • microparticles can be collected and the concentration thereof can be increased without using a large-scale apparatus such as a suction member and a control member for needles, and thus only solvent can be surely discharged out of the chamber.
  • Embodiment 2 Configuration of Fluid Handling Device Fluid handling device 200 according to Embodiment 2 differs from fluid handling device 100 according to Embodiment 1 only in that groove 210 is formed in the wall surface of the first region.
  • the configurations similar to those of fluid handling device 100 according to Embodiment 1 will be denoted with the same reference numerals, and the description thereof will be omitted.
  • FIG. 3A is a plan view of main body part 201 in the state where cover 102 is dismounted from fluid handling device 200 according to Embodiment 2 of the present invention.
  • FIG. 3B is a sectional view taken along line B-B of FIG. 3A.
  • chamber 120 includes first region 121a located on first opening 122 side and second region 121b located on second opening 123 side, and second region 121b has a height smaller than that of the first region 121a.
  • groove 210 extending from first opening 122 to second region 121b is formed.
  • Groove 210 functions as a channel for moving, toward second region 121b, only the solvent included in the fluid coming from first channel 110.
  • the height of groove 210 is not limited as long as the height of groove 210 is smaller than the diameter of a microparticle.
  • groove 210 is disposed at the same position as second region 121b in the height direction inside chamber 120, and the height of groove 210 is equal to the height of second region 121b.
  • Fluid Handling Method A fluid handling method using fluid handling device 200 is described below.
  • a microparticle manufacturing apparatus (not illustrated) or the like and fluid inlet 111 of fluid handling device 200 are connected with each other with a tube (not illustrated) or the like.
  • solvent outlet 131 of fluid handling device 200 and a container (not illustrated) or the like for collecting the solvent are connected with each other with a tube (not illustrated) or the like.
  • first channel 110, chamber 120, and second channel 130 are preliminarily filled with solvent that does not contain microparticles.
  • the fluid is introduced into chamber 120 through fluid inlet 111 and first channel 110.
  • a pressure application from fluid inlet 111 side and/or suction from solvent outlet 131 side may be performed to facilitate the flow of the fluid.
  • a part of the solvent included in the fluid having entered chamber 120 reaches second region 121b through groove 210, and moves to second opening 123 side (second channel 130 side).
  • the solvent included in the remaining fluid moves between the bottom surface of second region 121b and cover 102 toward second opening 123 side (second channel 130 side), and the microparticles in the remaining fluid stop in first region 121a.
  • the solvent is discharged from solvent outlet 131, and the microparticles are predominantly housed in chamber 120 (at a position on first region 121a side).
  • microparticles housed in chamber 120 of fluid handling device 200 can be output from chamber 120 as necessary by suction from microparticle outlet 112, for example.
  • microparticles can be collected and the concentration thereof can be increased without using a large-scale apparatus such as a suction member and a control member for needles, and thus only solvent can be surely discharged out of the chamber.
  • a large-scale apparatus such as a suction member and a control member for needles
  • the solvent included in the fluid can be efficiently carried to second opening 123 side (second channel 130 side) through groove 210, microparticles can be appropriately collected even when the flow velocity in chamber 120 is high.
  • Embodiment 3 Configuration of Fluid Handling Device Fluid handling device 300 according to Embodiment 3 differs from fluid handling device 100 according to Embodiment 1 only in that grooves 310 and protrusions 320 for blocking microparticles are formed at theboundary between first region 121a and second region 121b.
  • the configurations similar to those of fluid handling device 100 according to Embodiment 1 will be denoted with the same reference numerals, and the description thereof will be omitted.
  • FIG. 4A is a plan view of main body part 301 in the state where cover 102 is dismounted from fluid handling device 300 according to Embodiment 3 of the present invention.
  • FIG. 4B is a partially enlarged perspective view of the boundary between first region 121a and second region 121b that is indicated with the broken line in FIG. 4A.
  • FIG. 4C is a partially enlarged perspective view of the boundary in the state where fluid handling device 300 is used.
  • a plurality of grooves 310 extending along the height direction of the interior of chamber 120 are formed in the step surface between first region 121a and second region 121b. Grooves 310 open to first region 121a and second region 121b.
  • a plurality of protrusions 320 are disposed at respective positions corresponding to grooves 310 in regions near the step surface on the bottom surface second region 121b. As illustrated in FIG. 4C, grooves 310 and protrusions 320 have a function of blocking microparticles.
  • Grooves 310 are parallel to each other.
  • the shape and size of each groove 310 are not limited as long as microparticles 330 can be captured.
  • each groove 310 has a substantially half columnar shape. That is, groove 310 has an arcuate cross-sectional shape in a direction parallel to the bottom surface of second region 121b. The diameter of the arc is greater than the diameter of microparticle 330.
  • protrusion 320 is disposed for each grooves 310 on the bottom surface of second region 121b.
  • the shape and size of each protrusion 320 are not limited as long as entrance of microparticle 330 to second region 121b is prohibited.
  • protrusion 320 has a substantially cuboid shape.
  • grooves 310 and protrusions 320 are appropriately set in accordance with the size of microparticles 330 introduced into first region 121a and the like as illustrated in FIGS. 5A to 5D.
  • the number of grooves 310 may be equal to or different from that of protrusions 320.
  • Fluid Handling Method A fluid handling method using fluid handling device 300 is described below.
  • a microparticle manufacturing apparatus (not illustrated) or the like and fluid inlet 111 of fluid handling device 300 are connected with each other with a tube (not illustrated) or the like.
  • solvent outlet 131 of fluid handling device 300 and a container (not illustrated) or the like for collecting the solvent are connected with each other with a tube (not illustrated) or the like.
  • first channel 110, chamber 120 and second channel 130 are preliminarily filled with solvent that does not contain microparticles.
  • the fluid is introduced into chamber 120 through fluid inlet 111 and first channel 110.
  • a pressure application from fluid inlet 111 side and/or suction from solvent outlet 131 side may be performed to facilitate the flow of the fluid.
  • the fluid having entered chamber 120 moves to first region 121a.
  • the solvent in the fluid moves between the bottom surface of second region 121b and cover 102 toward second opening 123 side (second channel 130 side).
  • second channel 130 side second channel 130 side
  • microparticles housed in chamber 120 of fluid handling device 300 can be output from chamber 120 as necessary by suction from microparticle outlet 112, for example.
  • microparticles can be collected and the concentration thereof can be increased without using a large-scale apparatus such as a suction member and a control member for needles, and thus only solvent can be surely discharged out of the chamber.
  • a large-scale apparatus such as a suction member and a control member for needles
  • only solvent can be surely discharged out of the chamber.
  • movement of microparticle 330 to second region 121b can be more surely suppressed with grooves 310 and protrusions 320.
  • first channel 110 is connected with fluid inlet 111 and second channel 130 is connected with solvent outlet 131 in the embodiment, first channel 110 and/or second channel 130 may be connected with other chambers (not illustrated) provided in fluid handling device 100 or the like.
  • first region 121a and second region 121b is formed in a linear shape in plan view of chamber 120 in fluid handling device 100 of the Embodiments 1 and 2, the present invention is not limited to this.
  • the boundary between first region 121a and second region 121b may have a curved line shape (a shape of an arc whose center is located on first region 121a side) as illustrated in FIG. 6A, or the boundary between first region 121a and second region 121b may have a shape with two or more straight lines as illustrated in FIG. 6B.
  • grooves 310 described in Embodiment 3 may be formed in the step surfaces, or protrusions 320 may be disposed on the bottom surface of second region 121b.
  • groove 210 described in Embodiment 2 may be formed in the wall surface of first region 121a in main body parts 301, 401 and 501 of fluid handling device 300 of Embodiment 3 and fluid handling devices 400 and 500 of the modification.
  • the fluid handling device and the fluid handling method of the present invention are applicable to laboratory tests, food tests, environment tests and the like, for example.
  • Fluid handling device 101 201, 301, 401, 501 Main body part 102 Cover 110 First channel 110a First channel groove 111 Fluid inlet 112 Microparticle outlet 120 Chamber 120a Chamber recess 121a First region 121b Second region 122 First opening 123 Second opening 130 Second channel 130a Second channel groove 131 Solvent outlet 210 Groove 310 Groove 320 Protrusion 330 Microparticle

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

L'invention concerne un dispositif de manipulation de fluide qui peut contenir des microparticules telles que des gouttelettes tout en augmentant la concentration des microparticules. Un dispositif de manipulation de fluide comprend : une chambre comprenant une première ouverture et une seconde ouverture dans une surface de paroi ; un premier canal dont une extrémité est reliée à la première ouverture de la chambre ; et un second canal dont une extrémité est reliée à la seconde ouverture de la chambre. La chambre comprend une première région et une seconde région, la première région étant plus proche de la première ouverture que la seconde région, la seconde région étant plus proche de la seconde ouverture que la première région. Une hauteur d'un intérieur de la chambre est plus petite dans la seconde région que dans la première région.
PCT/JP2019/008412 2019-03-04 2019-03-04 Dispositif de manipulation de fluide WO2020178951A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2019/008412 WO2020178951A1 (fr) 2019-03-04 2019-03-04 Dispositif de manipulation de fluide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2019/008412 WO2020178951A1 (fr) 2019-03-04 2019-03-04 Dispositif de manipulation de fluide

Publications (1)

Publication Number Publication Date
WO2020178951A1 true WO2020178951A1 (fr) 2020-09-10

Family

ID=65818574

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2019/008412 WO2020178951A1 (fr) 2019-03-04 2019-03-04 Dispositif de manipulation de fluide

Country Status (1)

Country Link
WO (1) WO2020178951A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1019193B1 (fr) * 1997-03-27 2003-06-04 Biosite Diagnostics Inc. Dispositifs de diagnostic et appareil destine au deplacement regule de reactifs sans membranes
CN103959037A (zh) * 2011-10-25 2014-07-30 皇家飞利浦有限公司 从血液或其他介质中过滤颗粒
US9126160B2 (en) 2008-09-23 2015-09-08 Bio-Rad Laboratories, Inc. System for forming an array of emulsions
US20170157609A1 (en) * 2013-03-15 2017-06-08 Angle North America, Inc. Methods for Segregating Particles Using an Apparatus with a Size-Discriminating Separation Element Having an Elongate Leading Edge

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1019193B1 (fr) * 1997-03-27 2003-06-04 Biosite Diagnostics Inc. Dispositifs de diagnostic et appareil destine au deplacement regule de reactifs sans membranes
US9126160B2 (en) 2008-09-23 2015-09-08 Bio-Rad Laboratories, Inc. System for forming an array of emulsions
CN103959037A (zh) * 2011-10-25 2014-07-30 皇家飞利浦有限公司 从血液或其他介质中过滤颗粒
US20170157609A1 (en) * 2013-03-15 2017-06-08 Angle North America, Inc. Methods for Segregating Particles Using an Apparatus with a Size-Discriminating Separation Element Having an Elongate Leading Edge

Similar Documents

Publication Publication Date Title
US6852284B1 (en) Liquid analysis cartridge
CN103543256A (zh) 检验单元和流体分析盒
JP6506907B2 (ja) 液体取扱装置
US20060204403A1 (en) Micro-fluidic fluid separation device and method
WO2020178951A1 (fr) Dispositif de manipulation de fluide
US20220105506A1 (en) Fluid-handling system and cartridge
JP2019113472A (ja) 秤取構造及びマイクロチップ
WO2020178947A1 (fr) Dispositif de manipulation de fluide
US11311881B2 (en) Fluid handling method, fluid handling device used in same, and fluid handling system
US20220055028A1 (en) Fluid handling system
US20210299652A1 (en) Liquid handling device and liquid handling method
US11446666B2 (en) Fluid handling device
JP6343553B2 (ja) 液体取扱装置
US20200353471A1 (en) Cartridge and fluid handling system including same
JP2022045741A (ja) 液体取扱装置
JP6863074B2 (ja) マイクロ流路チップ
KR102453700B1 (ko) 원심 분리 장치용 챔버 및 이를 포함하는 원심 분리 장치
JP2013164268A (ja) マイクロチップ
WO2020166436A1 (fr) Système de manipulation de fluide et cartouche utilisée dans celui-ci
JP2022090836A (ja) 液体取扱装置
US20230256434A1 (en) Cartridge and liquid handling device
WO2020183591A1 (fr) Procédé de manipulation de fluide, et dispositif de manipulation de fluide et système de manipulation de fluide utilisés pour le procédé
CA3231847A1 (fr) Cassette destinee a contenir une puce microfluidique
JP2022049409A (ja) 液体取扱装置

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 19712308

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 19712308

Country of ref document: EP

Kind code of ref document: A1