WO2020155523A1 - Method for solidifying heavy metal of coal gangue by using microorganism - Google Patents

Method for solidifying heavy metal of coal gangue by using microorganism Download PDF

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WO2020155523A1
WO2020155523A1 PCT/CN2019/091821 CN2019091821W WO2020155523A1 WO 2020155523 A1 WO2020155523 A1 WO 2020155523A1 CN 2019091821 W CN2019091821 W CN 2019091821W WO 2020155523 A1 WO2020155523 A1 WO 2020155523A1
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gangue
solidifying
heavy metal
water
inoculant
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PCT/CN2019/091821
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French (fr)
Chinese (zh)
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黄艳利
阮泽宇
翟文
李俊孟
董霁红
马昆
高华东
韩震
郭亚超
李巍
扎伊泽小赛米尔
张吉雄
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中国矿业大学
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Priority to AU2019427413A priority Critical patent/AU2019427413B2/en
Publication of WO2020155523A1 publication Critical patent/WO2020155523A1/en
Priority to ZA2021/05485A priority patent/ZA202105485B/en

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    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes

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  • the invention relates to the fields of mining engineering, microorganisms, chemistry and the like, and in particular to a method for solidifying heavy metals in coal gangue with microorganisms.
  • Coal gangue is the waste discharged during coal mining, washing and processing, accounting for about 15% of coal production. Coal gangue has become the solid waste with the largest discharge volume (about 1/4 of my country's industrial solid waste), the largest area, and the more serious pollution among various industrial waste residues in my country. At present, the comprehensive utilization rate of coal gangue in my country is only 30%.
  • the use of gangue filling technology is a method of processing coal gangue, but the gangue needs to be treated to prevent pollution.
  • the purpose of the present invention is to provide a method for solidifying heavy metals in coal gangue with microorganisms, which use microorganisms to process the gangue to prevent heavy metal pollution in the gangue.
  • a method for solidifying heavy metals in coal gangue with microorganisms includes the following steps:
  • the bacteria-injection net includes several inner tubes and side tubes on both sides.
  • the inner tubes are arranged in parallel, and the two ends of the inner tube are respectively connected with the side tubes on both sides. Connected, a valve is installed at the junction of the inner tube and the side tube, a number of nozzles are distributed on the inner tube, and a number of water sensors are placed between each inner tube;
  • S3 Arrange sprinkler 2 above the end of the transfer machine.
  • Sprinkler 2 is connected to the container containing the bacterial agent through a conveying pipeline.
  • a pumping device is installed on the conveying pipeline; when filling starts, open the pumping device and sprinkler 2 to make Bacteria attached to the gangue passing through the transfer machine;
  • the water accumulation area is detected by the water immersion sensor.
  • the water immersion sensor detects the water accumulation area, open the valves on the inner pipes on both sides of the water immersion sensor and the corresponding water sensor.
  • the adjacent nozzle one sprays the bacterial agent to the stagnant water area.
  • the step S1 specifically includes: measuring the heavy metal ion content of the gangue to be filled, selecting bacterial species according to the measurement result, formulating the bacterial agent, and placing it in a container, wherein the copper ion content is 2.0 mg/ L, the content of cobalt ion is 0.5mg/L, and the content of nickel ion is 0.5mg/L as the standard.
  • the copper ion exceeds the maximum multiple
  • the Sporosarcina koreensis UR47 strain is used.
  • the cobalt ion exceeds the maximum multiple
  • the Sporosarcina sp UR31 strain is used.
  • the Terrabacter tumescens strain is used when the ion exceeds the maximum multiple.
  • the inner pipe in the step S2 is connected by a nozzle, the nozzles on the adjacent inner pipes form an area of 10m ⁇ 10m, and the water immersion sensor is placed at the center of the area of 10m ⁇ 10m.
  • step S3 the relationship between the jet flow rate of the nozzle 2 and the conveying capacity of the transfer machine is
  • Q is the jet flow rate
  • k is the adhesion compensation coefficient
  • 1 ⁇ k ⁇ 1.5 G is the gangue conveying capacity
  • r is the gangue particle size
  • ⁇ is the gangue density.
  • the microbial agent is a mixed liquid of a 1:4 volume of bacteria and nutrient solution.
  • the nutrient solution is a mixed solution of urea and calcium chloride solution in equal proportions with equal concentrations.
  • the present invention has the following beneficial effects:
  • the method for solidifying heavy metals in coal gangue with microorganisms of the present invention can directly solidify the heavy metals in the gangue in the goaf by carrying out microbial attachment treatment before the gangue is filled; after filling, it can be used when there is water in the goaf Injecting the bacteria net to supplement the bacteria agent, realizes the secondary solidification, reduces the migration pollution of heavy metal ions, enhances the solidification effect, and has the characteristics of low pollution and simple handling.
  • FIG. 1 is a flowchart of the present invention
  • Figure 2 is a schematic diagram of the bacteria injection network of the present invention.
  • Figure 3 is a schematic diagram of the number of the valve and the water immersion sensor of the present invention.
  • 1 is the side steel pipe
  • 2 is the valve
  • 3 is the internal steel pipe
  • 4 is the nozzle one
  • 5 is the water immersion sensor.
  • the density of the gangue for filling treatment is 2000kg/m 3 , the average particle size is 10cm, and the gangue filling capacity is 200t/h.
  • the gangue adopts the "solid waste leaching toxicity leaching method horizontal vibration method" (HJ557-2009) and “Solid Waste Leaching Toxicity Leaching Method Sulfuric Acid Nitric Acid Method” (HJ/T299-2007) analyzed the samples and found that the copper ion content was 26.20mg/L, the cobalt ion content was 0.84mg/L, and the nickel ion The content is 4.72mg/L.
  • the length of the working face is 200m, and the advancing length of the working face is 1000m.
  • the measured copper ion is 13 times the limit value, cobalt ion is close to 2 times, and nickel ion is about 9.5 times.
  • Sporosarcina koreensis UR47 should be selected;
  • the side steel pipe 1 is connected to the internal steel pipe 3 through the valve 2, a total of 9 rows of valves 2 are arranged, the internal steel pipes 3 are connected with the nozzle 4, and the side steel pipe 1 is pushed along the working surface
  • a total of 100 steel pipes are arranged in the direction, and a total of 20 internal steel pipes 3 are arranged along the working surface direction.
  • the nozzles on the adjacent internal steel pipes 3 form an area of 10m ⁇ 10m, and the water immersion sensor 5 is placed in the center of the area of 10m ⁇ 10m.
  • the first steel pipe and the end steel pipe are not placed, and a total of 18 sensors are placed in each row. Each sensor monitors an area of 10m ⁇ 10m;
  • the first in the first row is A11, the second is A12, and so on;
  • the first in the first row is B11, the first in the first row is B12, and the second The first in the row is B21, and so on;
  • the first in the first row is C11, the second in the first row is B12, the first in the second row is B21, and so on;
  • the bacterial agent When filling the goaf with gangue, the bacterial agent is transported to the second nozzle of the transfer machine through the bacteria conveying pipeline, so that the bacterial agent is attached to the surface of the gangue, and the spray flow rate k is 1.2, and the injection flow rate is calculated to be 120L/min;

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Environmental & Geological Engineering (AREA)
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Abstract

A method for solidifying a heavy metal of coal gangue by using a microorganism, comprising: measuring the heavy metal ion content of gangue to be filled, selecting a strain according to the measurement result, and formulating an inoculant; arranging inoculant injecting pipes in a goaf; during filling, spraying the inoculant on gangue by means of a nozzle so as to perform solidification treatment; and after filling is completed, spraying the inoculant to a water accumulation place by means of an inoculant injecting pipeline to achieve secondary solidification. By means of the method for solidifying the heavy metal of coal gangue by using the microorganism of the present invention, the present invention performs microorganism attachment treatment before the filling of gangue, can directly solidify the heavy metal of gangue in the goaf, and after the filling is completed and when there is water in the goaf, can also use an inoculant injecting net to complement the inoculant, thereby achieving secondary solidification, reducing the migration pollution of the heavy metal ions, having a good solidification effect and little pollution, and is easy to implement and popularize.

Description

一种用微生物固化煤矸石重金属的方法Method for solidifying heavy metals in coal gangue with microorganisms 技术领域Technical field
本发明涉及采矿工程、微生物、化学等领域，具体涉及一种用微生物固化煤矸石重金属的方法。The invention relates to the fields of mining engineering, microorganisms, chemistry and the like, and in particular to a method for solidifying heavy metals in coal gangue with microorganisms.
背景技术Background technique
煤矸石是煤炭开采、洗选及加工过程中排放的废物，约占煤炭产量的15％。煤矸石已成为我国各种工业废渣中排放量最大(约占我国工业固体废物的1/4)、占地最多、污染较严重的固体废物。目前，我国煤矸石的综合利用率仅为30％。利用矸石充填技术是一种处理煤矸石的方法，但需要对煤矸石进行处理后以防止污染。Coal gangue is the waste discharged during coal mining, washing and processing, accounting for about 15% of coal production. Coal gangue has become the solid waste with the largest discharge volume (about 1/4 of my country's industrial solid waste), the largest area, and the more serious pollution among various industrial waste residues in my country. At present, the comprehensive utilization rate of coal gangue in my country is only 30%. The use of gangue filling technology is a method of processing coal gangue, but the gangue needs to be treated to prevent pollution.
发明内容Summary of the invention
本发明的目的在于提供一种用微生物固化煤矸石重金属的方法，利用微生物对矸石进行处理，以防止煤矸石中重金属的污染。The purpose of the present invention is to provide a method for solidifying heavy metals in coal gangue with microorganisms, which use microorganisms to process the gangue to prevent heavy metal pollution in the gangue.
为实现上述发明目的，本发明的技术方案具体如下：In order to achieve the above-mentioned purpose of the invention, the technical scheme of the invention is specifically as follows:
一种用微生物固化煤矸石重金属的方法，包括以下步骤：A method for solidifying heavy metals in coal gangue with microorganisms includes the following steps:
S1：测定待充填矸石的重金属离子含量，根据测定结果选择菌种，配制成菌剂，盛放于容器中；S1: Determine the content of heavy metal ions in the gangue to be filled, select bacterial species according to the measurement results, formulate them into bacterial agents, and place them in a container;
S2：将注菌网平铺在待充填矸石的采空区，注菌网包括若干内管和两侧的边管，内管平行排布，内管的两端分别与两侧的边管相连接，内管与边管相接处安装有阀门，内管上分布有若干喷头一，各内管之间放置有若干水浸传感器；S2: Spread the bacteria-injection net in the goaf area to be filled with gangue. The bacteria-injection net includes several inner tubes and side tubes on both sides. The inner tubes are arranged in parallel, and the two ends of the inner tube are respectively connected with the side tubes on both sides. Connected, a valve is installed at the junction of the inner tube and the side tube, a number of nozzles are distributed on the inner tube, and a number of water sensors are placed between each inner tube;
S3：将喷头二布置在转载机端头上方，喷头二通过输送管路与盛有菌剂的容器相连，输送管路上安装有泵送装置；充填开始时，开启泵送装置和喷头二，使菌剂附着在经过转载机的矸石上；S3: Arrange sprinkler 2 above the end of the transfer machine. Sprinkler 2 is connected to the container containing the bacterial agent through a conveying pipeline. A pumping device is installed on the conveying pipeline; when filling starts, open the pumping device and sprinkler 2 to make Bacteria attached to the gangue passing through the transfer machine;
S4：充填完成后，通过水浸传感器探测积水区域，当水浸传感器探测到积水区域后，打开探测到积水区域的该水浸传感器两侧内管的阀门和与该水浸传感器相邻的喷头一，将菌剂喷到积水区域，喷菌完成后，关闭喷头一，开启相邻内管两端的阀门，使多余菌剂流出。S4: After the filling is completed, the water accumulation area is detected by the water immersion sensor. When the water immersion sensor detects the water accumulation area, open the valves on the inner pipes on both sides of the water immersion sensor and the corresponding water sensor. The adjacent nozzle one sprays the bacterial agent to the stagnant water area. After the bacterial spray is completed, close the nozzle one and open the valves at both ends of the adjacent inner pipe to allow the excess bacterial agent to flow out.
作为进一步优选的技术方案，所述步骤S1具体包括：测定待充填矸石的重金属离子含量，根据测定结果选择菌种，配制成菌剂，盛放于容器中，其中，以铜离子含量2.0mg/L，钴离子含量0.5mg/L，镍离子含量0.5mg/L为标准，当铜离子超标倍数最大时采用 Sporosarcina koreensis UR47菌种，当钴离子超标倍数最大时采用Sporosarcina sp UR31菌种，当镍离子超标倍数最大时采用Terrabacter tumescens菌种。As a further preferred technical solution, the step S1 specifically includes: measuring the heavy metal ion content of the gangue to be filled, selecting bacterial species according to the measurement result, formulating the bacterial agent, and placing it in a container, wherein the copper ion content is 2.0 mg/ L, the content of cobalt ion is 0.5mg/L, and the content of nickel ion is 0.5mg/L as the standard. When the copper ion exceeds the maximum multiple, the Sporosarcina koreensis UR47 strain is used. When the cobalt ion exceeds the maximum multiple, the Sporosarcina sp UR31 strain is used. The Terrabacter tumescens strain is used when the ion exceeds the maximum multiple.
作为进一步优选的技术方案，所述步骤S2中的内管通过喷头一连接，相邻内管上的喷头一组成10m×10m的区域，水浸传感器放置于10m×10m区域的中心处。As a further preferred technical solution, the inner pipe in the step S2 is connected by a nozzle, the nozzles on the adjacent inner pipes form an area of 10m×10m, and the water immersion sensor is placed at the center of the area of 10m×10m.
作为进一步优选的技术方案，所述步骤S3中喷头二的喷射流量与转载机的输送能力的关系为As a further preferred technical solution, in the step S3, the relationship between the jet flow rate of the nozzle 2 and the conveying capacity of the transfer machine is
Figure PCTCN2019091821-appb-000001
Figure PCTCN2019091821-appb-000001
其中，Q为喷射流量，k为附着补偿系数，1≤k≤1.5，G为矸石输送能力，r为矸石粒径，ρ为矸石密度。Among them, Q is the jet flow rate, k is the adhesion compensation coefficient, 1≤k≤1.5, G is the gangue conveying capacity, r is the gangue particle size, and ρ is the gangue density.
作为进一步优选的技术方案，所述菌剂为菌种和营养液按体积1比4混合的混合液。As a further preferred technical solution, the microbial agent is a mixed liquid of a 1:4 volume of bacteria and nutrient solution.
作为进一步优选的技术方案，所述营养液为浓度相等的尿素和氯化钙溶液的等比例混合液。As a further preferred technical solution, the nutrient solution is a mixed solution of urea and calcium chloride solution in equal proportions with equal concentrations.
与现有技术相比，本发明的有益效果：Compared with the prior art, the present invention has the following beneficial effects:
本发明的一种用微生物固化煤矸石重金属的方法，在矸石充填前进行微生物附着处理，可直接对采空区的矸石重金属进行固化；充填后完成后，采空区有积水时还可利用注菌网补充菌剂，实现了二次固化，减少了重金属离子的迁移污染，增强了固化效果，具有污染小，处理简单的特点。The method for solidifying heavy metals in coal gangue with microorganisms of the present invention can directly solidify the heavy metals in the gangue in the goaf by carrying out microbial attachment treatment before the gangue is filled; after filling, it can be used when there is water in the goaf Injecting the bacteria net to supplement the bacteria agent, realizes the secondary solidification, reduces the migration pollution of heavy metal ions, enhances the solidification effect, and has the characteristics of low pollution and simple handling.
附图说明Description of the drawings
图1是本发明的流程图；Figure 1 is a flowchart of the present invention;
图2是本发明的注菌网络的示意图；Figure 2 is a schematic diagram of the bacteria injection network of the present invention;
图3是本发明的阀门与水浸传感器的编号示意图；Figure 3 is a schematic diagram of the number of the valve and the water immersion sensor of the present invention;
图2中：1为边部钢管，2为阀门、3为内部钢管、4为喷头一、5为水浸传感器。In Figure 2: 1 is the side steel pipe, 2 is the valve, 3 is the internal steel pipe, 4 is the nozzle one, and 5 is the water immersion sensor.
具体实施方式：detailed description:
下面参照附图对本发明做进一步描述。The present invention will be further described below with reference to the drawings.
实施例Example
参照图1-3，以某矿为例，充填处理的矸石密度为2000kg/m 3，平均粒径为10cm，矸石充填能力为200t/h，矸石采用《固体废物浸出毒性浸出方法水平震荡法》(HJ557-2009)及《固体废物浸出毒性浸出方法硫酸硝酸法》(HJ/T299-2007)对样品分析，测出铜离子含量 为26.20mg/L，钴离子含量为0.84mg/L，镍离子含量为4.72mg/L。工作面长度为200m，工作面推进长度为1000m。 Refer to Figure 1-3. Take a mine as an example. The density of the gangue for filling treatment is 2000kg/m 3 , the average particle size is 10cm, and the gangue filling capacity is 200t/h. The gangue adopts the "solid waste leaching toxicity leaching method horizontal vibration method" (HJ557-2009) and "Solid Waste Leaching Toxicity Leaching Method Sulfuric Acid Nitric Acid Method" (HJ/T299-2007) analyzed the samples and found that the copper ion content was 26.20mg/L, the cobalt ion content was 0.84mg/L, and the nickel ion The content is 4.72mg/L. The length of the working face is 200m, and the advancing length of the working face is 1000m.
(1)测定的铜离子为限定值的13倍，钴离子接近2倍，镍离子约为9.5倍，此时应选择Sporosarcina koreensis UR47菌种；(1) The measured copper ion is 13 times the limit value, cobalt ion is close to 2 times, and nickel ion is about 9.5 times. In this case, Sporosarcina koreensis UR47 should be selected;
(2)菌种培养至OD600＝0.8，以醋酸钙和尿素混合溶液为营养液，浓度均为1mol/L，将每100ml菌剂与400ml营养液混合，制成菌剂；(2) The strain is cultivated to OD600=0.8, and the mixed solution of calcium acetate and urea is used as the nutrient solution, and the concentration is both 1mol/L, and each 100ml of inoculum is mixed with 400ml of nutrient solution to make an inoculant;
(3)沿工作面方向布置注菌网络，边部钢管1通过阀门2连接至内部钢管3，阀门2共布置9排，内部钢管3之间用喷头4连接，边部钢管1沿工作面推进方向共布置100根，内部钢管3沿工作面方向共布置20根。相邻内部钢管3上的喷头一组成10m×10m的区域，在10m×10m的区域的中心放置水浸传感器5，首根钢管和末端钢管上不放置，每排共放置18个传感器。每个传感器监测10m×10m的区域；(3) Arrange the bacteria injection network along the direction of the working surface, the side steel pipe 1 is connected to the internal steel pipe 3 through the valve 2, a total of 9 rows of valves 2 are arranged, the internal steel pipes 3 are connected with the nozzle 4, and the side steel pipe 1 is pushed along the working surface A total of 100 steel pipes are arranged in the direction, and a total of 20 internal steel pipes 3 are arranged along the working surface direction. The nozzles on the adjacent internal steel pipes 3 form an area of 10m×10m, and the water immersion sensor 5 is placed in the center of the area of 10m×10m. The first steel pipe and the end steel pipe are not placed, and a total of 18 sensors are placed in each row. Each sensor monitors an area of 10m×10m;
(4)对阀门编号，第一排第一个为A11，第二个为A12，以此类推；对喷头编号，第一排第一个为B11，第一排第一个为B12，第二排第一个为B21，依次类推；对传感器编号，第一排第一个为C11，第一排第二个为B12，第二排第一个为B21，依次类推；(4) For the valve number, the first in the first row is A11, the second is A12, and so on; for the nozzle numbers, the first in the first row is B11, the first in the first row is B12, and the second The first in the row is B21, and so on; for the sensor number, the first in the first row is C11, the second in the first row is B12, the first in the second row is B21, and so on;
(5)向采空区充填矸石时，通过输菌管路将菌剂输送至转载机端头喷头二处，使菌剂附着在矸石表面，喷射流量
Figure PCTCN2019091821-appb-000002
k取1.2，计算出喷射流量为120L/min； (5) When filling the goaf with gangue, the bacterial agent is transported to the second nozzle of the transfer machine through the bacteria conveying pipeline, so that the bacterial agent is attached to the surface of the gangue, and the spray flow rate
Figure PCTCN2019091821-appb-000002
k is 1.2, and the injection flow rate is calculated to be 120L/min;
(6)以编号为C11的水浸传感器为例，当C11报警时，显示为此区域有积水，打开阀门A11、A21，喷头B11、B12、B21、B22，进行喷菌处理该区域，这个区域菌剂用量为1000L。喷菌完成后，关闭喷头，开启A12和A22，使多余菌剂流出。(6) Take the water immersion sensor numbered C11 as an example. When C11 alarms, it shows that there is water in this area. Open valves A11, A21, nozzles B11, B12, B21, and B22 to spray bacteria to treat the area. The amount of regional bacteria agent is 1000L. After spraying the bacteria, close the spray head and open A12 and A22 to allow the excess bacteria to flow out.

Claims (6)

  1. 一种用微生物固化煤矸石重金属的方法，其特征在于，包括以下步骤：A method for solidifying heavy metals in coal gangue with microorganisms is characterized in that it comprises the following steps:
    S1：测定待充填矸石的重金属离子含量，根据测定结果选择菌种，配制成菌剂，盛放于容器中；S1: Determine the content of heavy metal ions in the gangue to be filled, select bacterial species according to the measurement results, formulate them into bacterial agents, and place them in a container;
    S2：将注菌网平铺在待充填矸石的采空区，注菌网包括若干内管和两侧的边管，内管平行排布，内管的两端分别与两侧的边管相连接，内管与边管相接处安装有阀门，内管上分布有若干喷头一，各内管之间放置有若干水浸传感器；S2: Spread the bacteria-injection net in the goaf area to be filled with gangue. The bacteria-injection net includes several inner tubes and side tubes on both sides. The inner tubes are arranged in parallel, and the two ends of the inner tube are respectively connected with the side tubes on both sides. Connected, a valve is installed at the junction of the inner tube and the side tube, a number of nozzles are distributed on the inner tube, and a number of water sensors are placed between each inner tube;
    S3：将喷头二布置在转载机端头上方，喷头二通过输送管路与盛有菌剂的容器相连，输送管路上安装有泵送装置；充填开始时，开启泵送装置和喷头二，使菌剂附着在经过转载机的矸石上；S3: Arrange sprinkler 2 above the end of the transfer machine. Sprinkler 2 is connected to the container containing the bacterial agent through a conveying pipeline. A pumping device is installed on the conveying pipeline; when filling starts, open the pumping device and sprinkler 2 to make Bacteria attached to the gangue passing through the transfer machine;
    S4：充填完成后，通过水浸传感器探测积水区域，当水浸传感器探测到积水区域后，打开探测到积水区域的该水浸传感器两侧内管的阀门和与该水浸传感器相邻的喷头一，将菌剂喷到积水区域，喷菌完成后，关闭喷头一，开启相邻内管两端的阀门，使多余菌剂流出。S4: After the filling is completed, the water accumulation area is detected by the water immersion sensor. When the water immersion sensor detects the water accumulation area, open the valves on the inner pipes on both sides of the water immersion sensor and the corresponding water sensor. The adjacent nozzle one sprays the bacterial agent to the stagnant water area. After the bacterial spray is completed, close the nozzle one and open the valves at both ends of the adjacent inner pipe to allow the excess bacterial agent to flow out.
  2. 根据权利要求1所述的一种用微生物固化煤矸石重金属的方法，其特征在于，所述步骤S1具体包括：The method for solidifying heavy metals in coal gangue with microorganisms according to claim 1, wherein said step S1 specifically comprises:
    测定待充填矸石的重金属离子含量，根据测定结果选择菌种，配制成菌剂，盛放于容器中，其中，以铜离子含量2.0mg/L，钴离子含量0.5mg/L，镍离子含量0.5mg/L为标准，当铜离子超标倍数最大时采用Sporosarcina koreensis UR47菌种，当钴离子超标倍数最大时采用Sporosarcina sp UR31菌种，当镍离子超标倍数最大时采用Terrabacter tumescens菌种。Determine the content of heavy metal ions in the gangue to be filled, select strains according to the results of the measurement, prepare them into bacterial agents, and place them in a container. Among them, the content of copper ion is 2.0 mg/L, the content of cobalt ion is 0.5 mg/L, and the content of nickel ion is 0.5. The mg/L is the standard. When the copper ion exceeds the maximum multiple, the Sporosarcina koreensis UR47 strain is used, when the cobalt ion exceeds the maximum multiple, the Sporosarcina sp UR31 strain is used, and when the nickel ion exceeds the maximum multiple, the Terrabacter tumorescens strain is used.
  3. 根据权利要求1所述的一种用微生物固化煤矸石重金属的方法，其特征在于，所述步骤S2中的内管通过喷头一连接，相邻内管上的喷头一组成10m×10m的区域，水浸传感器放置于10m×10m区域的中心处。The method for solidifying coal gangue heavy metals with microorganisms according to claim 1, characterized in that the inner pipe in step S2 is connected by a nozzle, and the nozzles on adjacent inner pipes form an area of 10m×10m, The water sensor is placed in the center of the 10m×10m area.
  4. 根据权利要求1所述的一种用微生物固化煤矸石重金属的方法，其特征在于，所述步骤S3中喷头二的喷射流量与转载机的输送能力的关系为The method for solidifying coal gangue heavy metals with microorganisms according to claim 1, wherein the relationship between the spray flow rate of the nozzle two and the conveying capacity of the transfer machine in the step S3 is
    Figure PCTCN2019091821-appb-100001
    Figure PCTCN2019091821-appb-100001
    其中，Q为喷射流量，k为附着补偿系数，1≤k≤1.5，G为矸石输送能力，r为矸石粒径，ρ为矸石密度。Among them, Q is the jet flow rate, k is the adhesion compensation coefficient, 1≤k≤1.5, G is the gangue conveying capacity, r is the gangue particle size, and ρ is the gangue density.
  5. 根据权利要求1所述的一种用微生物固化煤矸石重金属的方法，其特征在于，所述菌剂为菌种和营养液按体积1比4混合的混合液。The method for solidifying heavy metals in coal gangue with microorganisms according to claim 1, characterized in that the inoculum is a mixture of bacteria and nutrient solution in a volume ratio of 1:4.
  6. 根据权利要求5所述的一种用微生物固化煤矸石重金属的方法，其特征在于，所述营养液为浓度相等的尿素和氯化钙溶液的等比例混合液。The method for solidifying heavy metals in coal gangue with microorganisms according to claim 5, wherein the nutrient solution is a mixed solution of urea and calcium chloride solution in equal proportions with equal concentrations.
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