WO2020152794A1 - Dispositif d'inspection à pointe de microéchantillonnage - Google Patents

Dispositif d'inspection à pointe de microéchantillonnage Download PDF

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Publication number
WO2020152794A1
WO2020152794A1 PCT/JP2019/001961 JP2019001961W WO2020152794A1 WO 2020152794 A1 WO2020152794 A1 WO 2020152794A1 JP 2019001961 W JP2019001961 W JP 2019001961W WO 2020152794 A1 WO2020152794 A1 WO 2020152794A1
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WO
WIPO (PCT)
Prior art keywords
inspection device
recess
chip
tip
micro
Prior art date
Application number
PCT/JP2019/001961
Other languages
English (en)
Japanese (ja)
Inventor
佳則 山口
暁鳴 竇
子誠 朱
Original Assignee
佳則 山口
暁鳴 竇
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 佳則 山口, 暁鳴 竇 filed Critical 佳則 山口
Priority to PCT/JP2019/001961 priority Critical patent/WO2020152794A1/fr
Publication of WO2020152794A1 publication Critical patent/WO2020152794A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components

Definitions

  • the present invention is for carrying out tests such as environmental tests typified by heavy metal measurements, biochemical tests typified by using urine test strips, clinical tests, and food tests typified by detecting residual pesticides.
  • the present invention relates to a micro sampling chip inspection device (hereinafter, also simply referred to as an inspection device).
  • Heavy metals such as cadmium, cobalt, mercury, copper, zinc, lead, arsenic, and magnesium are harmful to the human body when ingested in large quantities, and ingest the amount of heavy metals contained in water, soil, food, vegetables, rice, and drinking water into the body. It is very important to know before.
  • Various methods have been proposed and implemented as methods for measuring heavy metals (see Patent Documents 1 and 2).
  • the problem of residual pesticides caused by spraying pesticides on foods such as grains, vegetables and fruits is serious, and 23 children died in 2013 because monocrotophos remained on vegetables in India. Such a serious incident is happening.
  • being able to perform the necessary and appropriate tests on the spot is important in ensuring subsequent diagnosis and treatment policies, prevention, and security.
  • By being able to detect the test on the spot within a few minutes and measuring the disease, food safety, and the presence or absence of residual pesticides patients can be diagnosed and administered as soon as possible. Can guarantee the safety of.
  • test paper that have coloring reagents fixed to paper media such as filter paper.
  • no technique has been established that enables simple and quick inspections on the spot using test paper.
  • An inspection performed using electrochemical measurement or a test strip is performed by bringing the sample into contact with a sensor part such as a measuring electrode or a test strip. This is an important factor for reproducibility. Therefore, it is common to use a pipette to collect a certain amount of sample for testing. That is, an accurate concentration can be determined by guaranteeing a fixed amount of sample. Further, it is required to dispose an inspection part (chip) including electrodes in order to prevent infection of a measurement person such as an inspection technician at the time of measurement and to prevent contamination between samples.
  • a microsampling chip (hereinafter, also simply referred to as a chip) is held at the tip of a pipette-type inspection device main body, and the sample sucked into the chip is directly inspected by the inspection device main body.
  • a microsampling chip hereinafter, also simply referred to as a chip
  • an electrode for electrochemical measurement was arranged in the chip, and the chip was attached to the tip of the inspection device body.
  • the chip in order to ensure that a predetermined amount of sample is accurately inhaled into the chip, the chip must be attached to the tip of the inspection device body while maintaining airtightness.
  • the present invention has been made in view of the above problems, and a chip is attached to the tip of an inspection apparatus body while maintaining airtightness, and the inspection apparatus body and the chip are accurately positioned.
  • the purpose is.
  • a micro-sampling chip inspection apparatus has a suction port at a tip for sucking a sample, a base end is in fluid communication with the suction port, and a sample sucked from the suction port is accommodated.
  • a chip provided with a sensor part for inspecting a sample in the internal space, and the base end of the chip is held by a tip part, and a fluid is sucked from the suction port of the chip held by the tip part.
  • a tester main body having a built-in test part for testing a sample using the sensor part of the chip.
  • the base end surface of the chip is provided with a cylindrical first recess
  • the bottom surface of the recess is provided with a prismatic second recess.
  • the main body has a columnar portion that fits into the first recess and a prismatic portion that is provided at the tip of the columnar portion and that fits into the second recess at the tip. Then, by fitting the cylindrical portion of the inspection device body into the first recess, the pump mechanism communicates with the internal space of the tip in an airtight manner, and the prismatic portion of the inspection device body has the second portion.
  • the sensor part of the chip and the inspection part of the inspection device main body are arranged in a predetermined positional relationship by being fitted in the recess.
  • the second recess of the chip may have a trapezoidal sectional shape in a direction perpendicular to an axial direction connecting the tip end and the base end of the microsampling chip.
  • an O-ring may be attached to the outer periphery of the cylindrical portion of the inspection device body. Then, the airtightness of the mounting portion of the chip with respect to the inspection apparatus body is further improved.
  • the sensor unit is an electrode substrate for performing an electrochemical measurement, and the electrode substrate is arranged such that one end thereof is in direct contact with a sample sucked into the internal space of the chip and the other end thereof is The inspection is performed by inserting the other end of the electrode substrate reaching the inside of the second recess of the chip into the tip end surface of the prismatic portion of the inspection device body.
  • a terminal port for electrically connecting the portion to the electrode substrate may be provided.
  • the function of performing the electrochemical measurement of the sample inhaled into the chip can be mounted.
  • the sensor unit may be a test paper or the like that exhibits a color reaction depending on the concentration of a specific component in the sample.
  • the inspection apparatus main body may be provided with a function of capturing an image of the sensor unit in the chip or capturing an image.
  • the pump mechanism is communicated with the internal space of the chip in an airtight manner by fitting the cylindrical portion of the inspection device main body into the first recess,
  • the sensor unit of the chip and the inspection unit of the inspection device main body are configured to be arranged in a predetermined positional relationship by fitting the prismatic portion of the inspection unit into the second recess.
  • the tip is attached to the tip of the main body while keeping airtightness, and the inspection device main body and the chip are accurately positioned.
  • a micro-sampling chip inspection device 1 (hereinafter referred to as inspection device 1) includes a micro-sampling chip 2 (hereinafter referred to as chip 2) and an inspection device main body 4.
  • the tip end of the tip 2 is held by the tip end of the inspection device body 4.
  • a suction port 6 for sucking the sample is provided at the tip of the chip 2.
  • a cylindrical first recess 8 is provided on the base end surface of the chip 2, and a prism-shaped second recess 10 is further provided on the bottom surface of the first recess 8.
  • the shape of the second recess 10 is not particularly limited, but as shown in FIG. 3, the cross-sectional shape in the direction perpendicular to the axial direction connecting the tip end and the base end of the chip 2 may be a trapezoidal shape. If the cross-sectional shape of the second recess 10 is a trapezoidal directional shape, the mounting direction of the chip 2 on the inspection apparatus body 4 can be defined.
  • An electrode substrate 12 for measurement is provided as a sensor unit in the internal space of the chip 2.
  • the electrode substrate 12 is arranged at a position where one end of the electrode substrate 12 is in direct contact with the sample sucked into the internal space of the chip 2 from the suction port 6, and the other end reaches the second recess 10.
  • the electrode substrate 12 is, for example, for performing electrochemical measurement of a sample.
  • the inspection device body 4 has a function as a pipette for sucking and discharging liquid.
  • the inspection device body 4 contains a syringe portion 14 for sucking and discharging a liquid, and a measurement circuit board 22 for performing electrochemical measurement.
  • the tip portion of the inspection device body 4 has a cylindrical portion 24 that fits into the first recess 8 of the chip 2 and a shape that fits into the second recess 10 of the chip 2 and projects from the tip surface of the cylindrical portion 24.
  • a prismatic portion 26 is provided.
  • An O-ring 28 is attached to the outer circumference of the cylindrical portion 24.
  • a piston 16 is provided inside the syringe portion 14 so as to be slidable in one axial direction. By sliding the piston 16 in the syringe portion 14 with the tip 2 attached to the tip of the inspection apparatus body 4, the liquid can be sucked and discharged through the tip 2.
  • a piston rod 18 for driving the piston 16 projects from the base end surface of the inspection device body 4, and a disc-shaped pressing portion 20, for example, is provided at the end portion of the piston rod 18.
  • the piston rod 18 is biased toward the base end side (upper side in the drawing) by an elastic body such as a coil spring, and when the user presses the pressing portion 20 toward the front end side (lower side in the drawing).
  • the piston rod 18 is automatically returned to the base end side by the elastic force of the elastic body.
  • the user manually operates the piston 16, but the present invention is not limited to this, and the piston 16 may be operated by using an electric motor. ..
  • a hole 30 and a terminal port 32 communicating with the syringe portion 14 are provided on the tip surface of the prismatic portion 26 of the inspection device body 4.
  • the terminal port 32 is for inserting the end portion of the electrode substrate 12 on the base end side of the chip 2 to electrically contact the measurement circuit substrate 22 with the electrode of the electrode substrate 12.
  • the prismatic portion 26 of the inspection device body 4 When mounting the chip 2 on the inspection device body 4, the prismatic portion 26 of the inspection device body 4 is fitted into the second recess 10 of the chip 2 as shown in FIG.
  • the positional relationship of the chip 2 with respect to the inspection device body 4 is defined by the fitting structure of the second recess 10 and the prismatic portion 26.
  • the inside of the chip 2 is defined.
  • the proximal end portion (upper end portion in the drawing) of the electrode substrate 12 fixed to is inserted into the terminal port 32 of the inspection device body 4.
  • the measurement circuit substrate 22 is electrically connected to the electrode of the electrode substrate 12.
  • the cylindrical portion 24 of the inspection device body 4 is fitted into the first recess 8 of the chip 2.
  • the cylindrical portion 24 of the inspection device body 4 is fitted in the first recess 8 of the chip 2, so that the chip for the inspection device body 4 is inserted. It is possible to secure the airtightness of the mounting portion of No. 2.
  • a predetermined amount (for example, 10 ⁇ L) of the sample is automatically sucked by the user merely pushing down the pressing portion 20 and releasing the hand so that the suction amount from the suction port 6 of the chip 2 is always constant.
  • An operation unit 34 and a display unit 36 are provided on the outer surface (base end surface in this embodiment) of the inspection device body 4.
  • the operation unit 34 is an operation button for the user to perform operations such as turning the power on/off, starting measurement, and changing the information displayed on the display unit 36.
  • the display unit 36 is realized by, for example, a small liquid crystal display.
  • the display unit 36 may be configured by a touch panel, and the display unit 36 may also have the function of the operation unit 34. Both the operation unit 34 and the display unit 36 are electrically connected to the measurement circuit board 22.
  • the inspection device body 4 includes a power source.
  • the power source can be realized by, for example, a dry battery or a rechargeable battery.
  • the power supply supplies necessary power to the measurement circuit board 22 and the display unit 36.
  • the inspection device body 4 is provided with an external output section so that information can be output to an external device such as a personal computer by a wired communication means such as a USB (Universal Serial Bus) terminal or a wireless communication means. Good.
  • a wired communication means such as a USB (Universal Serial Bus) terminal or a wireless communication means. Good.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un dispositif d'inspection de pointe de microéchantillonnage comprenant : une pointe possédant une extrémité distale comprenant une ouverture d'aspiration permettant d'aspirer un échantillon, une extrémité proximale en communication fluidique avec l'ouverture d'aspiration et un espace interne dans lequel l'échantillon aspiré depuis l'ouverture d'aspiration est reçu et à l'intérieur duquel est située une unité de capteur permettant d'inspecter l'échantillon ; et un corps de dispositif d'inspection possédant une partie d'extrémité distale permettant de maintenir l'extrémité proximale de la pointe et possédant, construit en son sein, un mécanisme de pompe permettant d'aspirer un fluide depuis l'ouverture d'aspiration de la pointe maintenue par la partie d'extrémité distale, et une unité d'inspection permettant d'inspecter l'échantillon à l'aide de l'unité de capteur de la pointe. De plus, un premier évidement cylindrique est situé sur la surface sur l'extrémité proximale de la pointe, et un second évidement prismatique est situé sur la surface inférieure du premier évidement. Le corps de dispositif d'inspection possède, au niveau de la partie d'extrémité distale, une partie cylindrique qui s'ajuste au premier évidement et une partie prismatique disposée au niveau de l'extrémité distale de la partie cylindrique et qui s'ajuste au second évidement. Le raccord de la partie cylindrique du corps de dispositif d'inspection dans le premier évidement permet au mécanisme de pompe d'être dans une communication étanche à l'air soutenue avec l'espace interne de la pointe. L'ajustement de la partie prismatique du corps de dispositif d'inspection dans le second évidement conduit à la fourniture de l'unité de capteur de la pointe et de l'unité d'inspection du corps de dispositif d'inspection dans une relation de position prescrite.
PCT/JP2019/001961 2019-01-23 2019-01-23 Dispositif d'inspection à pointe de microéchantillonnage WO2020152794A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2019/001961 WO2020152794A1 (fr) 2019-01-23 2019-01-23 Dispositif d'inspection à pointe de microéchantillonnage

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2019/001961 WO2020152794A1 (fr) 2019-01-23 2019-01-23 Dispositif d'inspection à pointe de microéchantillonnage

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WO2020152794A1 true WO2020152794A1 (fr) 2020-07-30

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04248457A (ja) * 1991-02-04 1992-09-03 Omron Corp 生化学測定器及び酵素センサ
JPH075145A (ja) * 1993-06-16 1995-01-10 Ngk Spark Plug Co Ltd カートリッジ型分析装置及びカートリッジ
JP2005345464A (ja) * 2004-05-06 2005-12-15 Matsushita Electric Ind Co Ltd センサ、測定装置および測定方法
JP2012530928A (ja) * 2009-06-30 2012-12-06 セラジェム メディシス インコーポレイテッド 検出器と共に用いて生体物質を測定するセンサ及びこれを用いる装置
US8673126B2 (en) * 2012-02-27 2014-03-18 Tri Electronics, Inc Dynamic precious metal assay device

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04248457A (ja) * 1991-02-04 1992-09-03 Omron Corp 生化学測定器及び酵素センサ
JPH075145A (ja) * 1993-06-16 1995-01-10 Ngk Spark Plug Co Ltd カートリッジ型分析装置及びカートリッジ
JP2005345464A (ja) * 2004-05-06 2005-12-15 Matsushita Electric Ind Co Ltd センサ、測定装置および測定方法
JP2012530928A (ja) * 2009-06-30 2012-12-06 セラジェム メディシス インコーポレイテッド 検出器と共に用いて生体物質を測定するセンサ及びこれを用いる装置
US8673126B2 (en) * 2012-02-27 2014-03-18 Tri Electronics, Inc Dynamic precious metal assay device

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