WO2020125259A1 - 一种elisa芯片及其使用方法 - Google Patents

一种elisa芯片及其使用方法 Download PDF

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WO2020125259A1
WO2020125259A1 PCT/CN2019/116227 CN2019116227W WO2020125259A1 WO 2020125259 A1 WO2020125259 A1 WO 2020125259A1 CN 2019116227 W CN2019116227 W CN 2019116227W WO 2020125259 A1 WO2020125259 A1 WO 2020125259A1
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reactor
cylindrical section
micro
add
liquid
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PCT/CN2019/116227
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French (fr)
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谢孝民
孙玉平
郭金城
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苏州汶颢微流控技术股份有限公司
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept

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  • the invention relates to an ELISA chip and a using method thereof, which belong to the technical field of biological detection.
  • ELISA is an immunoassay (IA) based on the solid phase of antigens or antibodies and the enzyme labeling of antigens or antibodies. After adding the substrate for the enzyme reaction, the substrate is catalyzed by the enzyme into a colored product, and the amount of the product is directly related to the amount of the test substance in the specimen, thereby performing qualitative or quantitative analysis.
  • Various antigen components including small molecule haptens, can be used to prepare specific antisera or monoclonal antibodies. Using this antibody as a reagent can detect the corresponding antigen in the specimen, so the application range of immunoassay is extremely wide.
  • reaction tubes of traditional ELISA chips are straight, and the coating area of antigen or antibody is small.
  • a third-party liquid filler is needed to add liquid from the upper part to the reaction tube, and then use the first
  • the tripartite liquid dispenser takes the liquid away, which not only makes the operation cumbersome, but also causes the waste liquid to be handled uncleanly, affecting the detection effect.
  • the present invention provides an ELISA chip and a method for using the same.
  • a micro-channel is provided in the lower part of the chip to draw the liquid from the lower part of the chip through a pump.
  • the reactor can be further installed into the interior
  • the stepped cylindrical structure can not only increase the coating area of antigen or antibody, but also reduce the influence of external light sources on the detection.
  • the first object of the present invention is to provide an ELISA chip including a chip body
  • the chip body includes a first surface and a second surface which are oppositely arranged, and a plurality of reactors are arranged between the first surface and the second surface, and the reactor is arranged from the first surface to the second surface Blind hole;
  • the reactor includes a first cylindrical section and a second cylindrical section arranged coaxially.
  • the inner diameter of the first cylindrical section is smaller than that of the second cylindrical section.
  • the top surface of the first cylindrical section overlaps the first surface and is an opening. , And set the opening as a sample port;
  • the second surface is provided with a micro-channel and a liquid outlet, the micro-channel is connected to the bottoms of several second cylindrical segments, and one end of the micro-channel is connected to the liquid outlet, and the liquid outlet is connected to the pump , You can use the pump to pump the liquid in the reactor.
  • the diameter of the first cylindrical section is 0.5-6 mm.
  • the diameter of the second cylindrical section is 0.6-50 mm.
  • the first surface is made of opaque material. It is further preferably made of black material.
  • the second surface is made of transparent material.
  • the material arrangement of the first surface and the second surface is beneficial to the optical path collection of the entire chip.
  • reactors are arranged linearly on the chip. Conducive to automatic sample setting.
  • micro flow channels are arranged in a tree shape, the reactor is set at the branch end of the micro flow channels, and the liquid outlet is set at the main end of the micro flow channels.
  • the second object of the present invention is to provide the method for using the ELISA chip, including the following steps: adding antigen, antibody, coating solution, blocking solution or washing solution to the reactor through the sample inlet, and then passing through the microchannel The liquid after the reaction or washing is removed.
  • the method specifically includes the following steps:
  • step (2) Add the sample to be tested or the control sample to the reactor through the sample inlet for incubation; use the washing method of step (2) to wash;
  • step (2) Add enzyme-labeled antibody to the reactor through the sample port for incubation, and then use the washing method of step (2) to wash;
  • the invention is provided with a micro-channel in the lower part of the chip to draw the liquid from the lower part of the chip through the pump, which is convenient to operate and reduces the risk of operation.
  • the reactor can be further arranged into a stepped cylindrical structure, not only It can increase the coating area of antigen or antibody, and can also reduce the influence of external light sources on detection.
  • Figure 1 is a schematic diagram of a prior art reactor
  • FIG. 2 is a schematic structural diagram of the chip of the present invention.
  • FIG. 3 is a schematic structural view of the chip reactor of the present invention.
  • FIG. 4 is a schematic diagram of the first surface of the chip of the present invention.
  • FIG. 5 is a schematic diagram of a reactor containing coated antigens or antibodies of the present invention.
  • FIG. 6 is a schematic diagram of photoelectric detection of the reactor of the present invention.
  • an ELISA chip of the present invention includes a chip body 1.
  • the chip body 1 includes a first surface 2 and a second surface 3 that are oppositely disposed, and between the first surface 2 and the second surface 3 is provided.
  • the reactor 4 is a blind hole provided from the first surface 2 to the second surface 3, an opening is set on the first surface, and the opening is set as the sample adding port 43; a sealing set on the second surface ;
  • Reactor 4 includes a first cylindrical section 41 and a second cylindrical section 42 arranged coaxially, the inner diameter of the first cylindrical section 41 is smaller than the second cylindrical section 42, the diameter of the first cylindrical section 41 is 0.5 ⁇ 6mm, the second cylindrical The diameter of the segment 42 is 0.6-50 mm;
  • the second surface 3 is provided with a micro flow channel 5 and a liquid outlet 6, the micro flow channel 5 communicates with the bottoms of several second cylindrical segments 42, and one end of the micro flow channel 5 is connected with the liquid The port 6 is connected, and the liquid outlet 6 is connected with
  • the arrangement of the first cylindrical section and the second cylindrical section of the present invention can not only increase the coating area of the antigen or antibody, but also reduce the influence of other external light sources on the detection result, see FIG. 6 as shown in FIG. 6 :
  • An ordinary reactor cannot block the interference light 10
  • the optical signal detector 8 not only absorbs the light emitted by the normal light source 9, the interference light 10 is also absorbed by the optical signal detector 8; and the diameter of the first cylindrical section of the present invention If it is smaller than the second cylindrical section, the interference light 10 irradiated from the side will be blocked by the first cylindrical section and cannot be absorbed by the optical signal detector 8, which can play a role in shielding interference.
  • the first surface 2 is made of an opaque black material
  • the second surface 3 is made of a transparent material.
  • the material setting of the first surface and the second surface is beneficial to the optical path collection of the entire chip.
  • the reactors 4 of the present invention are arranged linearly on the chip. Conducive to automatic sample setting.
  • the micro-channel 5 of the present invention is arranged in a tree shape, the reactor 4 is disposed at the branch end of the micro-channel 5, and the liquid outlet 6 is disposed at the main end of the micro-channel 5.
  • the method of using the chip of the present invention includes the following steps: adding antigen, antibody, coating solution, blocking solution or washing solution to the reactor through the sample inlet, and then reacting or washing the Liquid removal.
  • the specific steps include:
  • step (2) Add the sample to be tested or the control sample to the reactor through the sample inlet for incubation; use the washing method of step (2) to wash;
  • step (2) Add enzyme-labeled antibody to the reactor through the sample inlet for incubation, and then use the washing method of step (2) to wash;

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Abstract

一种ELISA芯片，包括芯片本体（1），芯片本体（1）包含相对设置的第一表面（2）与第二表面（3），第一表面（2）与第二表面（3）之间设有若干个反应器（4），反应器（4）为第一表面（2）向第二表面（3）设置的盲孔；反应器（4）包括同轴设置的第一圆柱段（41）和第二圆柱段（42），第一圆柱段（41）的内径小于第二圆柱段（42），第一圆柱段（41）的顶面与第一表面（2）重叠，为开口设置，并将开口设置为加样口（43）；第二表面（3）设有微流道（5）和出液口（6），微流道（5）与若干第二圆柱段（42）底部相连通，且微流道（5）一端与出液口（6）相连接，出液口（6）与泵进行连接。

Description

一种ELISA芯片及其使用方法 技术领域
本发明涉及一种ELISA芯片及其使用方法，属于生物检测技术领域。
背景技术
ELISA是一种免疫测定(immunoassay，IA)，基础是抗原或抗体的固相化及抗原或抗体的酶标记。加入酶反应的底物后，底物被酶催化成为有色产物，产物的量与标本中受检物质的量直接相关，由此进行定性或定量分析。由于各种抗原成份，包括小分子的半抗原，均可用以制备特异性的抗血清或单克隆抗体，利用此抗体作为试剂就可检测标本中相应的抗原，因此免疫测定的应用范围极广。
但是传统的ELISA芯片的反应管都是直筒式，抗原或抗体的包被区域较小，并且传统的是需要借助第三方加液器，从上部向反应管中添加液体，然后再从上部用第三方加液器将液体取走，这样不仅操作繁琐，还会导致废液处理不干净，影响检测效果。
发明内容
为解决上述技术问题，本发明提供一种ELISA芯片及其使用方法，在芯片下部设置微流道，将液体从芯片下部通过泵抽走，且通过上述设置后，可以进一步将反应器设置成内部呈阶梯式的筒状结构，不仅能够达到增加抗原或抗体的包被面积，还能够起到减少外界光源对检测的影响。
本发明的第一个目的是提供一种ELISA芯片，包括芯片本体，
所述的芯片本体包含相对设置的第一表面与第二表面，所述的第一表面与第二表面之间设有若干个反应器，所述反应器为第一表面向第二表面设置的盲孔；
所述的反应器包括同轴设置的第一圆柱段和第二圆柱段，第一圆柱段的内径小于第二圆柱段，所述第一圆柱段的顶面与第一表面重叠，为开口设置，并将开口设置为加样口；
所述第二表面设有微流道和出液口，所述的微流道与若干第二圆柱段底部相连通，且微流道一端与出液口相连接，出液口与泵进行连接，可以用过泵将反应器中的液体抽走。
进一步地，所述的第一圆柱段的直径为0.5～6mm。
进一步地，所述的第二圆柱段的直径为0.6～50mm。
进一步地，所述的第一表面采用不透明材料制备而成。进一步优选为黑色材料制成。
进一步地，所述的第二表面采用透明材料制备而成。第一表面与第二表面的材料设置，有利于整个芯片的光路采集。
进一步地，所述的反应器在芯片上按照线性排列。有利于自动加样设置。
进一步地，所述的微流道采用树形分布设置，所述的反应器设置在微流道的分支末端，所述的出液口设置在微流道的主干端。
本发明的第二个目的是提供所述的ELISA芯片的使用方法，包括如下步骤：通过加样口向反应器中加入抗原、抗体、包被液、封闭液或洗涤液，然后通过微流道将反应或洗涤后的液体去除。
进一步地，所述方法具体包括如下步骤：
(1)用包被液将抗原稀释，通过加样口向反应器中加入抗原，包被0.5～12h；
(2)通过加样口向反应器中加入洗涤液，洗涤之后通过微流道将洗涤液去除，洗涤2～3次；
(3)通过加样口向反应器中加入封闭液，封闭一定时间，然后通过微流道将封闭液去除；
(4)通过加样口向反应器中加入待测样品或对照样品进行孵育；采用步骤(2)洗涤方法进行洗涤；
(5)通过加样口向反应器中加入酶标抗体进行孵育，然后采用步骤(2) 洗涤方法进行洗涤；
(6)通过加样口向反应器中加入底物显色液，反应后加入终止液，然后进行判断。
本发明的有益效果是：
本发明在芯片下部设置微流道，将液体从芯片下部通过泵抽走，操作方便，降低操作风险，且通过上述设置后，可以进一步将反应器设置成内部呈阶梯式的筒状结构，不仅能够达到增加抗原或抗体的包被面积，还能够起到减少外界光源对检测的影响。
附图说明
图1为现有技术的反应器示意图；
图2为本发明芯片的结构示意图；
图3为本发明芯片反应器的结构示意图；
图4为本发明芯片第一表面示意图；
图5为本发明含有包被的抗原或抗体的反应器的示意图；
图6为本发明反应器光电检测示意图。
具体实施方式
下面结合附图和具体实施例对本发明作进一步说明，以使本领域的技术人员可以更好地理解本发明并能予以实施，但所举实施例不作为对本发明的限定。
实施例1：
结合附图2和3，本发明的一种ELISA芯片，包括芯片本体1，芯片本体1包含相对设置的第一表面2与第二表面3，第一表面2与第二表面3之间设有若干个反应器4，反应器4为第一表面2向第二表面3设置的盲孔，在第一表面上采用开口设置，并将开口设置为加样口43；第二表面上为封口设置；反应器4包括同轴设置的第一圆柱段41和第二圆柱段42，第一圆柱段41的内径小于第二圆柱段42，第一圆柱段41的直径为0.5～6mm，第二圆柱段42的直径为0.6～50mm；第二表面3设有微流道5和出液口6，微流道5与若干第二圆柱段42的底部相连通，且微流道5一端与出液口6相连接，出液口6连接有泵，可 以用过泵将反应器4中的液体抽走。
本发明的第一圆柱段和第二圆柱段的设置，不仅能够增加抗原或抗体的包被面积，还能够起到减少外界其他光源对检测结果的影响，参见附图6，如图6所示：普通的反应器不能够阻挡干扰光10，光信号检测器8不仅吸收到正常光源9发出的光，干扰光10也会被光信号检测器8吸收；而本发明的第一圆柱段的直径小于第二圆柱段，从侧面照射进来的干扰光10就会被第一圆柱段挡住，不能被光信号检测器8吸收到，能够起到屏蔽干扰的作用。
本发明第一表面2采用不透明的黑色材料制备而成，第二表面3采用透明材料制备而成，第一表面与第二表面的材料设置，有利于整个芯片的光路采集。
本发明的反应器4在芯片上按照线性排列。有利于自动加样设置。
本发明的微流道5采用树形分布设置，反应器4设置在微流道5的分支末端，出液口6设置在微流道5的主干端。
在使用时，本发明的芯片的使用方法，包括如下步骤：通过加样口向反应器中加入抗原、抗体、包被液、封闭液或洗涤液，然后通过微流道将反应或洗涤后的液体去除。
具体在采用间接法时，具体包括如下步骤：
(1)用包被液将抗原稀释，通过加样口向反应器中加入抗原，包被0.5～12h；
(2)通过加样口向反应器中加入洗涤液，洗涤之后通过微流道将洗涤液去除，洗涤2～3次；
(3)通过加样口向反应器中加入封闭液，封闭一段时间，然后通过微流道将封闭液去除；
(4)通过加样口向反应器中加入待测样品或对照样品进行孵育；采用步骤(2)洗涤方法进行洗涤；
(5)通过加样口向反应器中加入酶标抗体进行孵育，然后采用步骤(2)洗涤方法进行洗涤；
(6)通过加样口向反应器中加入底物显色液，反应后加入终止液，然后进行判断。
以上所述实施例仅是为充分说明本发明而所举的较佳的实施例，本发明的保护范围不限于此。本技术领域的技术人员在本发明基础上所作的等同替代或变换，均在本发明的保护范围之内。本发明的保护范围以权利要求书为准。

Claims (9)

  1. 一种ELISA芯片，其特征在于，包括芯片本体，
    所述的芯片本体包含相对设置的第一表面与第二表面，所述的第一表面与第二表面之间设有若干个反应器，所述反应器为第一表面向第二表面设置的盲孔；
    所述的反应器包括同轴设置的第一圆柱段和第二圆柱段，第一圆柱段的内径小于第二圆柱段，所述第一圆柱段的顶面与第一表面重叠，为开口设置，并将开口设置为加样口；
    所述第二表面设有微流道和出液口，所述的微流道与若干第二圆柱段底部相连通，且微流道一端与出液口相连接，出液口与泵进行连接。
  2. 根据权利要求1所述的一种ELISA芯片，其特征在于，所述的第一圆柱段的直径为0.5～6mm。
  3. 根据权利要求1所述的一种ELISA芯片，其特征在于，所述的第二圆柱段的直径为0.6～50mm。
  4. 根据权利要求1所述的一种ELISA芯片，其特征在于，所述的第一表面采用不透明材料制备而成。
  5. 根据权利要求1所述的一种ELISA芯片，其特征在于，所述的第二表面采用透明材料制备而成。
  6. 根据权利要求1所述的一种ELISA芯片，其特征在于，所述的反应器在芯片上按照线性排列。
  7. 根据权利要求1所述的一种ELISA芯片，其特征在于，所述的微流道采用树形分布设置，所述的反应器设置在微流道的分支末端，所述的出液口设置在微流道的主干端。
  8. 一种权利要求1～7任一项所述的ELISA芯片的使用方法，其特征在于，包括如下步骤：通过加样口向反应器中加入抗原、抗体、包被液、封闭液或洗涤液，然后通过微流道将反应或洗涤后的液体去除。
  9. 根据权利要求8所述的方法，其特征在于，具体包括如下步骤：
    (1)用包被液将抗原稀释，通过加样口向反应器中加入抗原，进行包被；
    (2)通过加样口向反应器中加入洗涤液，洗涤之后通过微流道将洗涤液去除，洗涤2～3次；
    (3)通过加样口向反应器中加入封闭液，进行封闭，然后通过微流道将封闭液去除；
    (4)通过加样口向反应器中加入待测样品或对照样品进行孵育；采用步骤(2)洗涤方法进行洗涤；
    (5)通过加样口向反应器中加入酶标抗体进行孵育，然后采用步骤(2)洗涤方法进行洗涤；
    (6)通过加样口向反应器中加入底物显色液，反应后加入终止液，然后进行判断。
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Publication number Priority date Publication date Assignee Title
CN109590036B (zh) * 2018-12-17 2024-04-19 苏州汶颢微流控技术股份有限公司 一种elisa芯片及其使用方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1781036A (zh) * 2002-02-12 2006-05-31 尤纳克西斯巴尔策斯有限公司 包括亚微米空心空间的部件
CN104360060A (zh) * 2014-11-14 2015-02-18 国家纳米科学中心 一种基于微流控芯片的肺炎支原体和流感病毒特异抗体IgM的检测方法
CN106984368A (zh) * 2017-03-30 2017-07-28 安徽理工大学 一种基于泵阀控制的乙肝快检微流控芯片及分析方法
US20170274382A1 (en) * 2014-03-31 2017-09-28 Nikon Corporation Supporting device and inspection method
US20180071736A1 (en) * 2016-01-26 2018-03-15 Lidong Qin Microfluidic Aliquot Chip For Single-Cell Isolation
CN109590036A (zh) * 2018-12-17 2019-04-09 苏州汶颢微流控技术股份有限公司 一种elisa芯片及其使用方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1517999A (en) * 1997-10-15 1999-05-03 Aclara Biosciences, Inc. Laminate microstructure device and method for making same
WO2005024436A1 (ja) * 2003-09-02 2005-03-17 Nec Corporation カスタマイズ可能なチップおよびその製造方法
WO2005066613A1 (en) * 2003-12-31 2005-07-21 President And Fellows Of Harvard College Assay device and method
JP2012163491A (ja) * 2011-02-08 2012-08-30 Institute Of Physical & Chemical Research 生化学反応用ピン、及びその利用
CN103018434B (zh) * 2012-12-05 2016-01-20 博源诺信（北京）生物科技有限责任公司 一种多指标检测装置及一种试剂盒，及其应用
CN103983779A (zh) * 2014-05-12 2014-08-13 重庆医科大学 一种用于重症患者病情评估的elisa试剂盒及其检测方法
CA3029905C (en) * 2016-07-12 2021-11-30 EMULATE, Inc. Uses of microfluidic devices comprising additive channels
CN108126765A (zh) * 2017-11-27 2018-06-08 深圳华炎微测医疗科技有限公司 Elisa检测微流控芯片和elisa检测微流控芯片体系以及它们的应用
CN209791574U (zh) * 2018-12-17 2019-12-17 苏州汶颢微流控技术股份有限公司 一种elisa芯片

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1781036A (zh) * 2002-02-12 2006-05-31 尤纳克西斯巴尔策斯有限公司 包括亚微米空心空间的部件
US20170274382A1 (en) * 2014-03-31 2017-09-28 Nikon Corporation Supporting device and inspection method
CN104360060A (zh) * 2014-11-14 2015-02-18 国家纳米科学中心 一种基于微流控芯片的肺炎支原体和流感病毒特异抗体IgM的检测方法
US20180071736A1 (en) * 2016-01-26 2018-03-15 Lidong Qin Microfluidic Aliquot Chip For Single-Cell Isolation
CN106984368A (zh) * 2017-03-30 2017-07-28 安徽理工大学 一种基于泵阀控制的乙肝快检微流控芯片及分析方法
CN109590036A (zh) * 2018-12-17 2019-04-09 苏州汶颢微流控技术股份有限公司 一种elisa芯片及其使用方法

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