WO2020100703A1

WO2020100703A1 - Liquid oral composition

Info

Publication number: WO2020100703A1
Application number: PCT/JP2019/043602
Authority: WO
Inventors: 智哉佐藤
Original assignee: 花王株式会社
Priority date: 2018-11-14
Filing date: 2019-11-07
Publication date: 2020-05-22
Also published as: TW202023519A; JP2020083879A

Abstract

The present invention pertains to a liquid oral composition that is capable of exerting excellent washing performance to thereby remove dental plaque firmly adhering to the tooth surface and, at the same time, efficiently preventing the adhesion of dental plaque to the tooth surface. Namely, the present invention pertains to a liquid oral composition comprising: (A) a fatty acid having a hydrocarbon group with 10-22 carbon atoms, inclusive, which at least partly contains an unsaturated fatty acid; and (B) one or more kinds of basic compounds selected from among an organic amine, an alkali metal hydroxide, a carbonate, a bicarbonate and a sesquicarbonate, and having a pH of 9.6-11 inclusive at 25°C.

Description

Liquid oral composition

The present invention relates to a liquid oral composition.

In the various oral compositions, fatty acids or salts thereof have been widely known as components that can bring about desired effects. Among them, fatty acid soaps such as sodium salts and potassium salts of fatty acids are used as detergents, as in the dentifrice composition described in Patent Document 1, for example.

On the other hand, in the oral composition described in Patent Document 2, a fatty acid salt is blended as a tooth surface coating agent, and in the oral compositions described in Patent Documents 3 to 4, the fatty acid or its salt and desensitization are used. The agent, or a fatty acid and a basic amino acid, forms a salt to block the dentin tubules and exert the effect of reducing the symptoms of dentin hypersensitivity.

(Patent Document 1) JP 2009-40756 A (Patent Document 2) JP 2000-103726 A (Patent Document 3) JP 2005-124216 A (Patent Document 4) JP 2014-62066 A

The present invention comprises the following components (A) and (B):
(A) Fatty acid having a hydrocarbon group having 10 to 22 carbon atoms, which contains unsaturated fatty acid at least in part (B) From organic amine, alkali metal hydroxide, carbonate, bicarbonate, and sesquicarbonate The present invention provides a liquid oral composition containing one or more selected basic compounds and having a pH at 25 ° C of 9.6 or more and 11 or less.

By the way, in attempting to realize a useful oral composition capable of maintaining a good oral environment while using a fatty acid, it is possible to remove plaque firmly adhered to the tooth surface by exerting high cleaning ability. Moreover, it is desired that the composition can effectively suppress the adhesion of plaque to the tooth surface even after that.
However, although the composition described in Patent Document 1 may be able to exert the plaque removal effect to some extent, further improvement is required in order to exert the plaque adhesion suppression effect. On the other hand, even the compositions described in Patent Documents 2 to 4 have not been examined in terms of removing plaque and suppressing adhesion, and there is room for improvement.

That is, the present invention is capable of removing the plaque firmly adhered to the tooth surface by exerting an excellent cleaning ability, and also capable of effectively suppressing the adhesion of the plaque to the tooth surface. It relates to a composition for oral cavity.

Therefore, the present inventor, as a result of earnest studies to solve the above problems, while using a specific fatty acid and a specific base in combination, by controlling the pH to a specific value, exhibits an excellent cleaning ability. The present inventors have found a liquid oral composition capable of effectively removing the plaque attached to the tooth surface and effectively suppressing the attachment of the plaque to the tooth surface.

According to the liquid composition for oral cavity of the present invention, immediately after being applied to the oral cavity, it exhibits excellent cleaning ability, and it is possible not only to remove the plaque firmly attached to the tooth surface but also in the oral cavity. It is possible to effectively suppress the adhesion of plaque to the tooth surface while being gradually diluted with saliva.

Detailed Description of the Invention

Hereinafter, the present invention will be described in detail.
The liquid oral composition of the present invention comprises the following components (A) and (B):
(A) Fatty acid having a hydrocarbon group having 10 to 22 carbon atoms, which contains unsaturated fatty acid at least in part (B) From organic amine, alkali metal hydroxide, carbonate, bicarbonate, and sesquicarbonate It contains one or more selected basic compounds and has a pH at 25 ° C. of 9.6 or more and 11.0 or less.

Since the liquid composition for oral cavity of the present invention comprises a combination of the fatty acid of component (A) and the basic compound of component (B) in a high pH range, immediately after being applied to the oral cavity, the component ( By mainly adsorbing to the plaque and rapidly detaching it from the tooth surface, A) mainly forms a micelle-like aggregate and can effectively remove the plaque adhered to the tooth surface. Then, as the composition is gradually diluted with saliva in the oral cavity and transitions to a low pH range, component (A) and component (B) form a vesicle-like aggregate, which densely covers the tooth surface. Thus, it is possible to effectively prevent the plaque from adhering to the tooth surface again or newly.
As described above, the present inventor makes the component (A) and the component (B) coexist in a state where the pH of the composition is controlled in a specific high region in advance, and the pH of the composition is adjusted by dilution with saliva during use. It has been discovered that, by transitioning to a low region, it is possible to unexpectedly achieve both a plaque removal effect and a plaque adhesion suppression effect.

The present invention contains, as the component (A), a fatty acid having a hydrocarbon group having 10 to 22 carbon atoms, the unsaturated fatty acid being contained at least in part. The number of carbon atoms of the hydrocarbon group contained in the component (A) fatty acid is 10 or more, preferably 12 or more, more preferably 14 or more, and further preferably 18 from the viewpoint of enhancing the effect of removing plaque. It is above, is 22 or less, and is preferably 20 or less. Examples of such a hydrocarbon group include a saturated hydrocarbon group, an unsaturated hydrocarbon group, a linear hydrocarbon group, and a hydrocarbon group having a branched chain. Specific examples include capric acid, lauric acid, and myristic acid. , Pentadecylic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidonic acid and behenic acid.

Further, the component (A) contains a fatty acid having an unsaturated hydrocarbon group at least in part, that is, an unsaturated fatty acid. Examples of such unsaturated hydrocarbon groups include one or more selected from palmitoleic acid, oleic acid, linoleic acid, linolenic acid, and the like. Among them, one or more selected from palmitoleic acid, oleic acid, and linoleic acid are preferable, and oleic acid is more preferable, from the viewpoint of well achieving both the effect of removing plaque and the effect of suppressing adhesion of plaque.

The content of the unsaturated fatty acid is preferably 15% by mass or more, more preferably 20% by mass or more in the component (A) from the viewpoint of achieving both the effect of removing plaque and the effect of suppressing adhesion of plaque. And more preferably 25 mass% or more, still more preferably 30 mass% or more, still more preferably 35 mass% or more, still more preferably 50 mass% or more, and even more preferably It is 55% by mass or more, more preferably 75% by mass or more, even more preferably 90% by mass or more, still more preferably 95% by mass or more.

The content of the component (A) exerts an excellent cleaning effect in a high pH region in the oral cavity, and from the viewpoint of effectively removing plaque adhered to the tooth surface, the liquid oral composition of the present invention In the above, it is preferably 0.05% by mass or more, more preferably 0.1% by mass or more, further preferably 0.2% by mass or more, and still more preferably 0.4% by mass or more. , And more preferably 0.5% by mass or more. In addition, the content of the component (A) is such that when the composition is diluted with saliva in the oral cavity and transitions to a low pH range, it forms a vesicle-like association with the component (B) described below to form plaque. From the viewpoint of sufficiently exhibiting the adhesion suppressing effect, in the liquid oral composition of the present invention, it is preferably 5% by mass or less, more preferably 3% by mass or less, and further preferably 1.5% by mass or less. And more preferably 1% by mass or less. The content of the component (A) in the liquid oral composition of the present invention is preferably 0.05 to 5% by mass, more preferably 0.1 to 3% by mass, and further preferably 0.2 to 1.5% by mass, more preferably 0.2 to 1% by mass, even more preferably 0.4 to 1% by mass, still more preferably 0.5 to 1% by mass. %.

The present invention contains, as the component (B), one or more basic compounds selected from organic amines, alkali metal hydroxides, carbonates, bicarbonates, and sesquicarbonates. By containing such component (B), as it is diluted with saliva after application in the oral cavity, it forms a vesicle-like association with component (A) that had formed a micelle-like association, The effect of suppressing plaque adhesion can be exerted.

Specific examples of the organic amine include one or more basic amino acids selected from arginine, lysine, and histidine; monoethanolamine, diethanolamine, triethanolamine, morpholine, triisopropanolamine, 2- Examples thereof include one or more alkanolamine compounds selected from amino-2-methyl-1,3-propanediol and 2-amino-2-methyl-1-propanol.

Specific examples of the alkali metal hydroxide include one or more selected from sodium hydroxide, potassium hydroxide, calcium hydroxide, and lithium hydroxide.

Specific examples of the carbonate, bicarbonate, and sesquicarbonate include one or two selected from sodium carbonate, potassium carbonate, calcium carbonate, sodium bicarbonate, potassium bicarbonate, and sodium sesquicarbonate. The above is mentioned.

Among them, as the component (B), when the composition is diluted with saliva in the oral cavity and transitions from the high pH range to the low pH range, the vesicle-like association with the component (A) is favorably and rapidly formed. From the viewpoint, one or more basic compounds selected from basic amino acids and alkanolamine compounds are preferable, and arginine or 2-amino-2-methyl-1-propanol is more preferable.

The content of the component (B) is such that when the composition is diluted with saliva in the oral cavity and transitions to a low pH range, it forms a vesicle-like association with the component (A) to suppress the plaque adhesion. From the viewpoint of sufficiently exhibiting, in the liquid oral cavity composition of the present invention, preferably 0.01% by mass or more, more preferably 0.1% by mass or more, and further preferably 0.17% by mass or more. And more preferably 0.25 mass% or more. In addition, the content of the component (B) in the liquid oral composition of the present invention is to be exhibited in a high pH region in the oral cavity, mainly from the viewpoint of ensuring an excellent cleaning effect by the component (A). In addition, it is preferably 3% by mass or less, more preferably 2% by mass or less, further preferably 1% by mass or less, and further preferably 0.8% by mass or less. The content of the component (B) in the liquid oral composition of the present invention is preferably 0.01 to 3% by mass, more preferably 0.01 to 2% by mass, and further preferably The amount is 0.1 to 1% by mass, more preferably 0.17 to 0.8% by mass, and still more preferably 0.25 to 0.8% by mass.

The molar ratio ((B) / (A)) of the component (B) and the component (A) is preferably 0.5 to 40 from the viewpoint of achieving both a plaque removing effect and a plaque adhesion suppressing effect. Yes, more preferably 0.9 to 30, still more preferably 0.95 to 30, still more preferably 0.95 to 20, even more preferably 1 to 20, and even more preferably It is 1.5 to 20, more preferably 1.5 to 15, and even more preferably 2 to 10.

The liquid composition for oral cavity of the present invention contains water (C) from the viewpoint that each component can be well dispersed or dissolved and well diffused in the oral cavity while maintaining good shape retention. Is preferred. The water of the component (C) in the present invention includes not only purified water and the like mixed in the composition for liquid oral cavity but also each of the mixed components such as 70% sorbitol liquid (aqueous solution) used in the formulation. The total moisture contained in the liquid oral composition, including the moisture contained therein. The content of such water (C) in the liquid oral composition of the present invention is preferably 50% by mass or more, more preferably 70% by mass or more, further preferably 80% by mass or more, It is preferably 99% by mass or less, more preferably 97% by mass or less, and further preferably 95% by mass or less.

The liquid oral composition of the present invention is a vesicle-like aggregate of the component (A) and the component (B) when the composition is diluted with saliva in the oral cavity and transitions from a high pH range to a low pH range. It is preferable to limit the content of free divalent metal ions from the viewpoint of not inhibiting the formation of the plaque and the effect of suppressing the adhesion of plaque. Specific examples of the free divalent metal ion include calcium ion and magnesium ion. The content of such free divalent metal ions in the liquid oral composition of the present invention is preferably 0.5% by mass or less, more preferably 0.1% by mass or less, and further preferably 0%. It is preferably 0.05% by mass or less, or the liquid composition for oral cavity of the present invention does not contain a free divalent metal ion.
The content of the free divalent metal ion is a value measured as the total amount of the free divalent metal ion by an ICP emission analysis method (ICP emission analysis device: Optima 5300 DV manufactured by Perkin Elmer).

The liquid oral composition of the present invention, in addition to the above components, for example, anionic surfactants such as alkyl sulfate ester salts; polyoxyethylene hydrogenated castor oil, sucrose fatty acid ester, etc., within the range that does not impair the effects of the present invention. Nonionic surfactants; binding agents such as sodium alginate, carboxymethyl cellulose or salts thereof, hydroxyethyl cellulose, carrageenan, xanthan gum; bactericides such as isopropylmethylphenol and cetylpyridinium chloride; sugar alcohols such as sorbitol, xylitol, erythritol; glycerin A wetting agent such as; a preservative; a fluoride or other medicinal effect agent; a pigment; a dye;

The pH of the liquid oral composition of the present invention at 25 ° C. is 9.6 or more, preferably 9.8 or more, from the viewpoint of exhibiting an excellent cleaning effect by setting the pH environment in the oral cavity in a high pH range. And more preferably 10 or more, and further preferably 10.2 or more. In addition, the pH of the liquid oral composition of the present invention at 25 ° C. changes to a low pH range when the composition is diluted with saliva in the oral cavity, and exhibits an excellent effect of suppressing plaque adhesion. Therefore, it is 11 or less, preferably 10.8 or less, more preferably 10.6 or less, and further preferably 10.4 or less. The pH of the liquid oral composition of the present invention at 25 ° C. is 9.6 or more and 11 or less, preferably 9.8 to 10.8, more preferably 10 to 10.6, More preferably, it is 10.2 to 10.4.

In adjusting the pH of the liquid oral composition of the present invention to the above range, one kind selected from organic acids such as citric acid, malic acid, lactic acid, tartaric acid and succinic acid other than the above components or salts thereof. Alternatively, two or more kinds may be used as a pH adjusting agent.

The liquid oral composition of the present invention retains the composition in the oral cavity and is diluted with saliva in the oral cavity to remove the plaque adhering to the tooth surface while or after removing the dental plaque. It can be used for a method of suppressing the adhesion of plaque to the skin.

Thus, if the liquid oral composition of the present invention, not only for suppressing the adhesion of the oral biofilm, it can be used for the removal of the oral biofilm, effectively both effects Can be demonstrated.

With respect to the embodiments described above, the present invention further discloses the following liquid oral compositions.
[1] The following components (A) and (B):
(A) Fatty acid having a hydrocarbon group having 10 to 22 carbon atoms, which contains unsaturated fatty acid at least in part (B) From organic amine, alkali metal hydroxide, carbonate, bicarbonate, and sesquicarbonate A composition for liquid oral cavity containing one or more selected basic compounds and having a pH at 25 ° C of 9.6 or more and 11 or less.
[2] The carbon number of the hydrocarbon group contained in the fatty acid of the component (A) is preferably 12 or more, more preferably 14 or more, still more preferably 18 or more, preferably 20 or less, or The hydrocarbon group contained in the fatty acid of component (A) is preferably capric acid, lauric acid, myristic acid, pentadecyl acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidonic acid. And the liquid oral composition according to the above [1], which is one or more selected from behenic acid and the like.
[3] The unsaturated hydrocarbon group of the unsaturated fatty acid contained in the component (A) is preferably one or more selected from palmitoleic acid, oleic acid, linoleic acid, and linolenic acid, and more preferably palmitoleic. The liquid oral composition according to the above [1] or [2], which is one or more selected from acids, oleic acid and linoleic acid, and more preferably oleic acid.
[4] The content of the unsaturated fatty acid in the component (A) is preferably 15% by mass or more, preferably 20% by mass or more, more preferably 25% by mass or more, and more preferably 30 mass% or more, more preferably 35 mass% or more, further preferably 50 mass% or more, even more preferably 55 mass% or more, even more preferably 75 mass% or more, The composition for liquid oral cavity according to any one of [1] to [3] above, which is more preferably 90% by mass or more, and preferably 95% by mass or more.
[5] The content of the component (A) is preferably 0.05% by mass or more, more preferably 0.1% by mass or more, further preferably 0.2% by mass or more, and further preferably 0.4 mass% or more, more preferably 0.5 mass% or more, preferably 5 mass% or less, more preferably 3 mass% or less, and more preferably 1.5 mass% or less. The composition for liquid oral cavity according to any one of [1] to [4] above, which is 1% by mass or less.

[6] The component (B) is preferably one or more selected from basic amino acids and alkanolamine compounds, more preferably arginine or 2-amino-2-methyl-1-propanol. The liquid composition for oral cavity according to any one of [1] to [5] above.
[7] The content of the component (B) is preferably 0.01% by mass or more, more preferably 0.1% by mass or more, further preferably 0.17% by mass or more, and further preferably 0.25% by mass or more, preferably 3% by mass or less, more preferably 2% by mass or less, further preferably 1% by mass or less, and further preferably 0.8% by mass or less. The liquid composition for oral cavity according to any one of [1] to [6] above.
[8] The molar ratio ((B) / (A)) of the component (B) and the component (A) is preferably 0.5 to 40, more preferably 0.9 to 30, and further preferably. 0.95 to 30, more preferably 0.95 to 20, further preferably 1 to 20, further preferably 1.5 to 20, and further preferably 1.5 to 15. The liquid oral composition according to any one of the above [1] to [7], which is more preferably 2 to 10.

[9] The pH at 25 ° C. is preferably 9.8 or higher, more preferably 10 or higher, still more preferably 10.2 or higher, preferably 10.8 or lower, more preferably 10 or higher. The liquid oral composition according to any one of the above [1] to [8], which is 0.6 or less, and more preferably 10.4 or less.
[10] The content of the free divalent metal ion is preferably 0.5% by mass or less, more preferably 0.1% by mass or less, further preferably 0.05% by mass or less, or The composition for liquid oral cavity according to any one of the above [1] to [9], which preferably contains no free divalent metal ion.
[11] The liquid oral composition according to any one of the above [1] to [10], wherein the free divalent metal ion is a calcium ion or a magnesium ion.
[12] The content of the component (C) is preferably 50% by mass or more, more preferably 70% by mass or more, further preferably 80% by mass or more, preferably 99% by mass or less, The liquid oral composition according to any one of the above [1] to [11], which is more preferably 97% by mass or less, and further preferably 95% by mass or less.

[13] The liquid oral composition according to any one of the above [1] to [12], which is a composition for suppressing adhesion of oral biofilm and for removing oral biofilm.
[14] Applying the liquid oral composition according to any one of [1] to [12] while gradually diluting it in the oral cavity with saliva, and removing the oral biofilm while suppressing the adhesion of the oral biofilm. Method.
[15] Use of the liquid oral composition according to any one of the above [1] to [12], for removing the oral biofilm while suppressing the adhesion of the oral biofilm.

Hereinafter, the present invention will be specifically described based on Examples. Unless otherwise specified in the table, the content of each component is% by mass.

[Examples 1 to 15 and Comparative Examples 1 to 3]
A liquid oral composition was prepared according to the formulation shown in Table 1. Each of the obtained liquid oral compositions had a content of free divalent metal ions of 0.05% by mass or less. Using each of the obtained liquid oral compositions, each evaluation was performed according to the following methods.
The results are shown in Table 1.

[Evaluation of plaque removal rate]
1) Collection of stimulated saliva Targeting healthy men in their twenties to thirties, chew the gum pellets contained in Dentbuff Strip (made by Oral Care Co., Ltd.) and spit saliva accumulated in the mouth into the falcon tube each time. Then, saliva was collected in the Falcon tube. Since there are individual differences in the bacteria in saliva, the saliva of one healthy man was used to evaluate all the examples and comparative examples.

2) Preparation of dental plaque model Saliva collected in a falcon tube was centrifuged at 3000 rpm / 25 ° C / 10min (centrifuge device name: cooling centrifuge CF15RN, manufactured by HITACHI). Using the separated supernatant saliva, sucrose was added so as to be a 5 mass% solution, and then stirred using a stirring device (voltex, manufactured by Nippon Genetics Co., Ltd.) to prepare a plaque model test solution.
Next, one surface of the HAp substrate (manufactured by Cosmo Bio Co., Ltd., 1 cm square) was mirror-polished using polishing paper of 40 μm, 12 μm, and 3 μm, and then immersed in 1N HCl for 1 minute for acid deashing treatment. The HAp plate after the treatment was washed with ion-exchanged water, dried, put in a 24-well plate, and 1 mL of the prepared plaque model test solution was added thereto. Then, the HAp substrate was stored in a plastic case together with a CO ₂ pack under anaerobic conditions and incubated at 37 ° C. for 48 hours.

3) Evaluation of plaque removal effect Using a vacuum pump, saliva in the plate was absorbed, 1 mL of ion-exchanged water was added, and the mixture was shaken for 5 minutes. Next, water was absorbed using a pump, and 0.13 mL of artificial saliva was added to 1 mL of each composition obtained in Examples and Comparative Examples to separately prepare a 24-well plate. Then, the HAp substrate on which the model plaque was formed was immersed in the prepared solution and shaken for 1 minute. Shaking was performed using a shaker (BioShake iQ (manufactured by Waken Betech Co.)) at room temperature (25 ° C.) and 500 rpm.
Then, each composition was absorbed, 1 mL of ion-exchanged water was added, and the mixture was shaken for 5 minutes, and this was repeated twice. Next, water was absorbed, 750 μL of a 0.1 mass% crystal violet (CV) solution was added, and the mixture was shaken for 15 minutes.
Further, the CV staining solution was sucked with a pump, 1 mL of ion-exchanged water was added, and the mixture was shaken for 5 minutes, and this was repeated twice. Then, suck up the water with a pump, add 500 μL of ethanol and pipette, dilute the extract 10 times with ion-exchanged water, and use a microplate recorder (TECAN wavelength tunable absorption microplate reader Sunrise Rainbow Thermo). Absorbance OD _{595 nm} was measured.
Further, without using the composition obtained above, the plaque removal rate (%) was calculated according to the following formula, using the absorbance OD _{595 nm} (initial value) just washed with ion-exchanged water as a reference.
The larger the obtained plaque removal rate value, the higher the plaque removal effect.
Plaque removal ratio (%) = 100- × 100 { OD 595nm only washed with OD _{595 nm} / ion-exchanged water at the time of using the resulting composition}

[Evaluation of plaque formation inhibition]
1) Treatment with the composition One side of the HAp substrate (1 cm square manufactured by Cosmo Bio Co., Ltd.) was mirror-polished using 40 μm, 12 μm, 3 μm polishing paper, and then acid decalcified by immersion in 1N HCl for 1 minute. gave. The HAp plate after the treatment was washed with ion-exchanged water, dried, put in a 24-well plate, 1 mL of each composition obtained in Examples and Comparative Examples, and 0.13 mL of artificial saliva were added, and shaken for 5 minutes. . Shaking was performed using a shaker (BioShake iQ (manufactured by Waken Betech Co.)) at room temperature (25 ° C.) and 500 rpm. Thereafter, each composition was absorbed, 1 mL of ion-exchanged water was added, and the mixture was shaken for 5 minutes, and then water was absorbed to obtain a treated substrate.

2) Collection of stimulated saliva Targeting healthy men in their 20s to 30s, chew the gum pellets contained in Dent Buff Strip (made by Oral Care Co., Ltd.) and spit saliva accumulated in the mouth into a falcon tube each time. Then, saliva was collected in the Falcon tube. Since there are individual differences in the bacteria in saliva, a plaque formation inhibition test was carried out for all Examples and Comparative Examples using saliva of one healthy male.

3) Formation of model plaque The saliva collected in a falcon tube was centrifuged at 3000 rpm / 25 ° C / 10min using a cooling centrifuge (CF15RN, manufactured by HITACHI). Using the separated supernatant saliva, sucrose was added so as to be a 5 mass% solution, and then stirred using a stirring device (voltex, manufactured by Nippon Genetics Co., Ltd.) to prepare a plaque model test solution.
Next, 1 mL each of the above-prepared dental plaque model test solution was added to the HAp substrate treated in 1), and this was stored in a plastic case together with a CO ₂ pack under anaerobic conditions at 37 ° C. for 48 hours. Incubated for hours.

4) Evaluation of Plaque Formation Inhibitory Effect Using a vacuum pump, saliva in the plate was absorbed, 1 mL of ion-exchanged water was added, and the mixture was shaken once for 5 minutes. Next, water was absorbed using a pump, 750 μL of a 0.1 mass% crystal violet (CV) solution was added, and the mixture was shaken for 15 minutes.
Further, the CV staining solution was sucked with a pump, 1 mL of ion-exchanged water was added, and the mixture was shaken for 5 minutes, and this was repeated twice. Then, suck up the water with a pump, add 500 μL of ethanol and pipette, dilute the extract 10 times with ion-exchanged water, and use a microplate recorder (TECAN wavelength tunable absorption microplate reader Sunrise Rainbow Thermo). Absorbance OD _{595 nm} was measured.
Further, without using the composition obtained above, the plaque formation inhibition rate (%) was calculated according to the following formula, using the absorbance OD _{595 nm} (initial value) just washed with ion-exchanged water as a reference.
It should be noted that the larger the obtained plaque formation inhibiting rate, the higher the plaque formation inhibiting effect.
Plaque formation inhibition rate (%) = 100- × 100 { OD 595nm of OD _{595 nm} / unprocessed substrate board treated in the above obtained composition}

Claims

The following components (A) and (B):
(A) Fatty acid having a hydrocarbon group having 10 to 22 carbon atoms, which contains unsaturated fatty acid at least in part (B) From organic amine, alkali metal hydroxide, carbonate, bicarbonate, and sesquicarbonate A liquid composition for oral cavity containing one or more selected basic compounds and having a pH at 25 ° C of 9.6 or more and 11 or less.
The composition for liquid oral cavity according to claim 1, wherein the hydrocarbon group contained in the component (A) has 18 or more and 22 or less carbon atoms.
The liquid oral composition according to claim 1 or 2, wherein the content of the unsaturated fatty acid is 15% by mass or more in the component (A).
The liquid oral composition according to any one of claims 1 to 3, wherein the unsaturated fatty acid contained in the component (A) is one or more selected from palmitoleic acid, oleic acid and linoleic acid.
The liquid oral composition according to any one of claims 1 to 4, wherein the content of water (C) is 50% by mass or more.
The liquid composition for oral cavity according to any one of claims 1 to 5, wherein the content of free divalent metal ions is 0.5% by mass or less.
The liquid oral composition according to any one of claims 1 to 6, wherein the component (B) is one or more basic compounds selected from basic amino acids and alkanolamine compounds.
The liquid composition for oral cavity according to any one of claims 1 to 7, which is a composition for suppressing adhesion of oral biofilm and for removing oral biofilm.
The liquid oral composition according to any one of claims 1 to 8 is retained in the oral cavity and diluted with saliva in the oral cavity, while removing plaque adhering to the tooth surface or after the removal. A method of suppressing the adhesion of plaque to the tooth surface.