WO2020096156A1 - Novel lactobacillus gasseri skb1102 strain or cosmetic composition containing same having anti-pollution function - Google Patents

Novel lactobacillus gasseri skb1102 strain or cosmetic composition containing same having anti-pollution function Download PDF

Info

Publication number
WO2020096156A1
WO2020096156A1 PCT/KR2019/007585 KR2019007585W WO2020096156A1 WO 2020096156 A1 WO2020096156 A1 WO 2020096156A1 KR 2019007585 W KR2019007585 W KR 2019007585W WO 2020096156 A1 WO2020096156 A1 WO 2020096156A1
Authority
WO
WIPO (PCT)
Prior art keywords
strain
skin
skb1102
lactobacillus gasseri
effect
Prior art date
Application number
PCT/KR2019/007585
Other languages
French (fr)
Inventor
Kyung Min Kim
Du Seong Kim
Doo Sung HONG
Seung Hun Lee
Heui Jong Yu
Original Assignee
Sk Bioland Co., Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sk Bioland Co., Ltd filed Critical Sk Bioland Co., Ltd
Publication of WO2020096156A1 publication Critical patent/WO2020096156A1/en

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/002—Aftershave preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/007—Preparations for dry skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus

Definitions

  • the present invention relates to a Lactobacillus gasseri SKB1102 strain having an anti-pollution function against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals, or to a cosmetic composition containing the same.
  • the present invention relates to the above strain or a cosmetic composition having a skin irritation mitigation effect, a skin cell proliferation effect or a skin moisturizing effect containing the same.
  • the epidermis of the skin is a barrier for preventing the penetration of chemicals and the evaporation of water in the body.
  • the stratum corneum contains 10 to 25% water and the epidermal layer contains 70% water.
  • the pH of the skin shows slight acidity of 5.0 to 6.0 on the surface, but the pH of the dermal layer in the skin is neutral, similar to that of body fluids. Meanwhile, when the water content of the stratum corneum falls below 10% due to skin drying in winter or upon frequent cleansing with soap, or due to skin aging attributable to age and health, skin elasticity and glossiness may decrease and fine wrinkling and skin cracking may occur.
  • the human skin is exposed to fine dust and ultraviolet rays, thereby causing aging due to skin wrinkles, reduced elasticity, pigmentation, and the like, and damage to the skin barrier may result in decreased water content, keratinization, psoriasis and acne.
  • Fine dust or ultrafine dust which is one of the main causes of skin damage that has recently received attention, is a kind of particulate matter (PM) having a diameter of 2.5 to 10 ⁇ m or having a diameter of 2.5 ⁇ m or less, and directly impedes the skin barrier function and aggravates dermatitis. Since the particle size of fine dust is very small, it may penetrate into the skin through hair follicles, and thus may affect other persons, in addition to patients with atopic and allergic diseases or with sensitive skin.
  • PM particulate matter
  • Fine dust generates reactive oxygen species when penetrating into the skin to thus affect the skin, and thus, in order to protect the skin from fine dust, it is necessary to use basic cosmetics containing antioxidants to reduce the production of reactive oxygen species and prevent the effects thereof. Moreover, since fine dust itself may reduce the skin barrier function, it may be helpful to consume plenty of water and make use of moisturizers.
  • Cosmetic refers to a product in which a cosmetic ingredient and a medicinal ingredient are combined, and has been used by cosmetic professionals since the 1990s.
  • Dr. Albert Kligman started the development of cosmeceuticals.
  • Dr. Kligman discovered that retinoid, which is a compound having a vitamin A skeleton, is very effective for restoring the human skin.
  • Cosmeceutical products are used to play a role as functional cosmetics through the use of a filler, which is a plastic implant, or medically proven functional components for whitening, moisturizing, etc. These are mainly sold in types specialized for particular areas, such as serum, cream, oil, lip balm, and essence, which have the effects of sebum secretion control of acne-prone skin, keratin care, skin barrier enhancement function, and the like.
  • microorganisms serving as a material for a cosmeceutical product include lactic-acid bacteria.
  • lactic-acid bacteria exhibit immune enhancement effects in the human body through interactions with immune cells present in the intestinal mucosa after oral ingestion.
  • Most lactic-acid bacteria useful as probiotics are gram-positive bacteria, in which cell walls exist outside the cell membrane and the cell walls have a complex layer structure of peptidoglycan, lipid protein, and teichoic acid. These cell wall components bind to MAMP by the PRR (pattern recognition receptor) expressed in the intestinal mucosa of humans to thus cause an immune response.
  • PRR pattern recognition receptor
  • lactic-acid bacteria are used in some skin cosmetics.
  • a lactic-acid bacterium as a cosmetic ingredient for a cosmeceutical product, there is a need to scientifically prove the effects thereof, but it is merely applied to the extent necessary for fermented cosmetics or probiotic-fermented liquids, and is simply used for only a single product effect.
  • required is a formulation having a triple effect which stepwisely solves skin problems depending on the condition of damaged skin.
  • step 1 The annual arrival of yellow dust and the phenomenon of fine dust, which is an air pollutant attributable to industrialization, lead to a skin aging cascade, including increasing the sensitivity of the exposed skin (step 1), decreasing moisturizing capability due to the damage to the skin barrier (step 2), and decreasing elasticity due to reduced skin water content (step 3).
  • the present inventors analyzed the effects of fine dust, which causes the acceleration of skin sensitivity, on the skin barrier, and have developed a 3-in-1 formulation as a cosmeceutical material having all of anti-pollution cascade effects of fine-dust removal, skin moisturizing and skin elasticity when applied to the skin layer, using Lactobacillus gasseri SKB1102, which is a probiotic, thus culminating in the present invention.
  • Patent Document 0001 Korean Patent Application Publication No. 10-2014-0128675 (Title: Probiotics of Lactobacillus gasseri HY7025 having skin wrinkle inhibitory and moisturizing effects and product containing the same as active ingredient, Applicant: Korea Yakult Co. Ltd., Laid-open Date: November 06, 2014)
  • Patent Document 0002 Korean Patent Application Publication No. 10-2016-0038864 (Title: Method of producing microbial agent comprising intracellular accumulated aglycon therein and microbial agent produced thereby, Applicant: RNA Inc., Laid-open Date: April 07, 2016)
  • Patent Document 0003 Korean Patent Application Publication No. 10-2010-0054428 (Title: Extract for improving glucose homeostasis fermented using Lactobacillus gasseri KCTC 3163 from ginseng or ginseng extract and method of preparing the same, Applicant: Korea Food Research Institute, Laid-open Date: May 25, 2010)
  • Patent Document 0004 Korean Patent No. 10-1614141 (Title: Method of increasing yield of fermented ginseng seed oil, Applicant: Korea Food Research Institute, Registration Date: April 14, 2016)
  • an objective of the present invention is to provide a Lactobacillus gasseri SKB1102 strain having an anti-pollution function against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals, or a cosmetic composition containing the same.
  • an objective of the present invention is to provide the above strain or a cosmetic composition having a skin irritation mitigation effect, a skin cell proliferation effect or a skin moisturizing effect containing the same.
  • the present invention provides a Lactobacillus gasseri SKB1102 strain having an anti-pollution function.
  • the strain has an anti-pollution function against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals contained in the air.
  • the strain may have a skin irritation mitigation effect.
  • the strain may have an effect of inhibiting the expression of a gene selected from the group consisting of CYP1A1 (Cytochrome P450, family 1, member A1), interleukin-8 and interleukin-1 ⁇ , increased by an environmentally harmful factor.
  • CYP1A1 Cytochrome P450, family 1, member A1
  • interleukin-8 interleukin-1 ⁇
  • the strain may have an effect of proliferation of at least one skin cell selected from among a human fibroblast and a human keratinocyte.
  • the strain may have a skin moisturizing effect.
  • the strain may increase the expression of a moisturizing factor selected from among aquaporin-3 and CD-44.
  • the strain may have a skin elasticity improvement effect.
  • the strain may increase the production of collagen type I, which is a skin-elasticity-related factor.
  • the present invention provides a cosmetic composition having an anti-pollution function, a skin irritation mitigation effect, a skin cell proliferation effect or a skin moisturizing effect, the cosmetic composition comprising cells of the Lactobacillus gasseri SKB1102 strain, a culture broth thereof, or an extract thereof.
  • the cosmetic composition may be provided in the form of a formulation selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milky lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream, a foundation, an essence, a nourishing essence, a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.
  • a formulation selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milky lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream, a foundation, an essence, a nourishing essence, a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream
  • the Lactobacillus gasseri SKB1102 strain of the present invention may be cultured in an MRS agar medium or an MRS liquid medium at 25 to 40°C and a pH of 4.0 to 6.5 for 16 hours to 2 days.
  • the cosmetic composition of the present invention may contain cells of a Lactobacillus gasseri SKB1102 strain, a culture broth including the cells, a culture broth excluding the cells, or an extract thereof, and the extract may be obtained by extracting an extraction sample using an extraction solvent such as water, a C1 to C4 lower alcohol, propylene glycol, butylene glycol, glycerin or a mixture thereof.
  • the C1 to C4 lower alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol.
  • the extraction solvent used in the preparation of the extract may be used in a volume corresponding to 1 to 40 times (1 to 40 L relative to 1 kg) and preferably 5 to 40 times relative to the weight of a mixture.
  • the extraction process for obtaining the extract may be performed at 20 to 100°C for 1 min to 48 hr. This procedure may be repeated 1 to 4 times.
  • the extract may be dissolved in water and then additionally fractionated using at least one solvent selected from the group consisting of n-hexane, methylene chloride, acetone, chloroform, ethyl acetate and n-butanol, thus affording a fraction.
  • the temperature required to prepare the extract may fall in the range of 20 to 100°C, but the present invention is not limited thereto.
  • the extraction time is not particularly limited, but preferably falls within the range of 10 minutes to 2 days.
  • a typical extraction device an ultrasonication extractor or a fractionator may be used.
  • the filtration which is a process of removing suspended solid particles from the extract solution, may be performed by filtering particles using cotton, nylon, paper or the like, or through ultrafiltration, freeze filtration, centrifugation or the like, but the present invention is not limited thereto.
  • the extraction process for preparing the extract is not particularly limited, and for example, cold extraction, ultrasonic extraction, reflux cooling extraction, room-temperature extraction, hot-water extraction and the like may be used depending on the kind of extract and the type of extraction process.
  • the extract thus prepared may be dried through hot-air drying, drying under reduced pressure, freeze drying, vacuum drying, spray drying, foam drying, high-frequency drying, infrared drying and the like, but the present invention is not limited thereto. In some cases, a process of grinding the final dried extract may be additionally performed.
  • the extract may be used after purification through column chromatography.
  • the chromatography may be selected from among silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, medium-pressure liquid chromatography, thin-layer chromatography (TLC), silica gel vacuum liquid chromatography and high-performance liquid chromatography.
  • the strain of the present invention or the cosmetic composition containing the same has anti-pollution efficacy against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals contained in the air.
  • heavy metals are metals having a specific gravity of 4 or more, and may have a maximum specific gravity of 23, including mercury (13.5), cadmium (8.7), lead (11.3), copper (8.9) and the like.
  • the present invention addresses a cosmetic composition containing the Lactobacillus gasseri SKB1102, and the composition may contain 0.1 to 20 wt% of a Lactobacillus gasseri SKB1102 strain, a culture broth thereof, or an extract thereof.
  • the formulation of the cosmetic composition is not particularly limited, and is preferably selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milky lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream, a foundation, an essence, a nourishing essence, a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.
  • the cosmetic composition of the present invention may further contain a component selected from the group consisting of a water-soluble vitamin, an oil-soluble vitamin, a high-molecular-weight peptide, a high-molecular-weight polysaccharide, a sphingolipid and a seaweed extract.
  • any water-soluble vitamin may be used so long as it is capable of being blended in cosmetics, and preferable examples thereof include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C and vitamin H, and salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate, etc.) thereof are also included in the water-soluble vitamin that may be used in the present invention.
  • the water-soluble vitamin may be obtained using a typical method such as a microbial transformation method, a method of purification from a microbial culture broth, an enzymatic method, or a chemical synthesis method.
  • any oil-soluble vitamin may be used so long as it is capable of being blended in cosmetics, and preferable examples thereof include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (DL- ⁇ tocopherol, D- ⁇ tocopherol) and the like, and derivatives (ascorbyl palmitate, ascorbyl stearate, ascorbyl dipalmitate, DL- ⁇ tocopherol acetate, DL- ⁇ tocopherol nicotinate vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) thereof are also included in the oil-soluble vitamin that may be used in the present invention.
  • the oil-soluble vitamin may be obtained using a typical method such as a microbial transformation method, a method of purification from a microbial culture broth, an enzymatic method, or a chemical synthesis method.
  • any high-molecular-weight peptide may be used so long as it is capable of being blended in cosmetics, and preferable examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like.
  • the high-molecular-weight peptide may be obtained through purification using a typical method such as a method of purification from a microbial culture broth, an enzymatic method, or a chemical synthesis method, or may be used after purification from natural products such as the dermis of pigs and cows, the silk fiber of silkworms, etc.
  • any high-molecular-weight polysaccharide may be used so long as it is capable of being blended in the cosmetic composition, and preferable examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or salts thereof (sodium salt, etc.) and the like.
  • chondroitin sulfate or salts thereof may be typically used after purification from mammals or fish.
  • any sphingolipid may be used so long as it is capable of being blended in the cosmetic composition, and preferable examples thereof include ceramide, phytosphingosine and sphingoglycolipid.
  • the sphingolipid may be obtained from mammals, fish, shellfish, yeast or plants through purification using a typical method or through chemical synthesis.
  • any seaweed extract may be used so long as it is capable of being blended in the cosmetic composition, and preferable examples thereof include a brown algae extract, a red algae extract, a green algae extract, and the like, and also, carrageenan, alginic acid, sodium alginate, potassium alginate and the like, purified from the seaweed extract, are also included in the seaweed extract that may be used in the present invention.
  • the seaweed extract may be obtained from seaweed through purification using a typical method.
  • the cosmetic composition of the present invention may also contain additional components that are blended in typical cosmetics.
  • Examples of the additionally blended components may include fat and oil components, a moisturizer, an emollient, a surfactant, organic and inorganic pigments, an organic powder, a UV absorber, a preservative, a disinfectant, an antioxidant, a plant extract, a pH adjuster, an alcohol, a colorant, a fragrance, a blood circulation accelerator, a cooling agent, an antiperspirant, purified water and the like.
  • Examples of the fat and oil components may include ester-based fats and oils, hydrocarbon-based fats and oils, silicone-based fats and oils, fluorine-based fats and oils, animal fats and oils, vegetable fats and oils, etc.
  • ester-based fats and oils may include esters, such as glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diisopropyl adipate, isoalkyl neopentanoate, glyceryl tri(caprylate, caprate), trimethylolpropanoate
  • hydrocarbon-based fats and oils may include squalene, liquid paraffin, ⁇ -olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, Vaseline, and the like.
  • silicone-based fats and oils may include polymethylsilicone, methylphenylsilicone, methyl cyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethyl cyclosiloxane, dimethyl siloxane ⁇ methyl cetyloxysiloxane copolymer, dimethyl siloxane ⁇ methyl stearoxysiloxane copolymer, alkyl-modified silicone oil, amino-modified silicone oil, and the like.
  • fluorine-based fats and oils may include perfluoropolyether and the like.
  • animal or vegetable fats and oils may include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, safflower oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, grapeseed oil, cottonseed oil, coconut oil, kukui nut oil, wheat germ oil, rice germ oil, shea butter, evening primrose oil, macadamia nut oil, meadowfoam seed oil, egg yolk oil, beef tallow, horse oil, mink oil, orange roughy oil, jojoba oil, candelilla wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.
  • Examples of the moisturizer may include a water-soluble low-molecular-weight moisturizer, a fat-soluble low-molecular-weight moisturizer, a water-soluble high-molecular-weight moisturizer, a fat-soluble high-molecular-weight moisturizer, and the like.
  • Examples of the fat-soluble low-molecular-weight moisturizer may include cholesterol, cholesterol ester, and the like.
  • water-soluble high-molecular-weight moisturizer may include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, dextrin, and the like.
  • Examples of the fat-soluble high-molecular-weight moisturizer may include polyvinylpyrrolidone ⁇ eicosene copolymer, polyvinylpyrrolidone ⁇ hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, high-molecular-weight silicone, and the like.
  • emollient may include long-chain acylglutamate cholesteryl ester, cholesteryl hydroxystearate, 12-hydroxystearic acid, stearic acid, rhodinic acid, lanolin fatty acid cholesteryl ester, and the like.
  • the surfactant may include a nonionic surfactant, an anionic surfactant, a cationic surfactant, and an amphoteric surfactant.
  • nonionic surfactant may include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitol fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE ⁇ POP (polyoxyethylene ⁇ polyoxypropylene) copolymer, POE ⁇ POP alkyl ether, polyether-modified silicone, alkanolamide laurate, alkylamine oxide, hydrogenated soybean phospholipid, and the like.
  • anionic surfactant may include fatty acid soap, ⁇ -acyl sulfonate, alkyl sulfonate, alkylallyl sulfonate, alkylnaphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkylamide phosphate, alkyloylalkyltaurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphoric acid ester, and the like.
  • Examples of the cationic surfactant may include alkyl trimethylammonium chloride, stearyl trimethylammonium chloride, stearyl trimethylammonium bromide, cetostearyl trimethylammonium chloride, distearyl dimethylammonium chloride, stearyl dimethylbenzylammonium chloride, behenyl trimethylammonium bromide, benzalkonium chloride, diethyl aminoethylamide stearate, dimethyl aminopropylamide stearate, lanolin-derived quaternary ammonium salts, and the like.
  • amphoteric surfactant may include carboxybetaine-, amidobetaine-, sulfobetaine-, hydroxysulfobetaine-, amidosulfobetaine-, phosphobetaine-, aminocarboxylate-, imidazoline derivative- and amidoamine-type amphoteric surfactants.
  • organic and inorganic pigments may include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, iron oxide red, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, carmine, and complexes thereof; organic pigments such as polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicone resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinyl benzene ⁇ styrene copolymer, silk powder, cellulose, CI pigment yellow and CI pigment orange; and combinations of these inorgan
  • organic powder may include metal soap such as calcium stearate; alkylphosphoric acid metal salts such as sodium zinc cetylphosphate, zinc laurylphosphate and calcium laurylphosphate; acylamino acid polyvalent metal salts such as N-lauroyl- ⁇ -alanine calcium, N-lauroyl- ⁇ -alanine zinc and N-lauroylglycine calcium; amidosulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids such as N-e-lauroyl-L-lysine, N-e-palmitoyllysine, N- ⁇ -palmitoylornithine, N- ⁇ -lauroylarginine and N- ⁇ -hardened beef tallow fatty acid acylarginine; N-acylpolypeptides such as N-lauroylglycylglycine; ⁇ -amino
  • UV absorber may include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate, 2-ethoxyethyl para-methoxycinnamate, octyl para-methoxycinnamate, glyceryl mono-2-ethylhexanoate dipara-methoxycinnamate, isopropyl para-methoxycinnamate, diisopropyl diisopropylcinnamic acid ester mixtures, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxylmeth
  • Examples of the disinfectant may include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc pyrithione, benzalkonium chloride, photosensitizing dye No. 301, sodium mononitroguaiacol, undecylenic acid, and the like.
  • antioxidant examples include butylhydroxyanisole, propyl gallate, erythorbic acid, and the like.
  • Examples of the pH adjuster may include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium hydrogen phosphate, and the like.
  • Examples of the alcohol may include higher alcohols such as cetyl alcohol and the like.
  • the additionally blended components are not limited thereto, and any of them may be used within a range that does not impair the purposes and effects of the present invention, but the amount thereof is preferably 0.01 to 5 wt %, and more preferably 0.01 to 3 wt%, based on the total weight of the cosmetic composition.
  • the cosmetic composition of the present invention may be provided in the form of a solution, an emulsion, or a viscous mixture.
  • the cosmetic composition of the present invention may contain, in addition to the extract as the active ingredient, components typically used in cosmetics, examples of which may include a typical adjuvant and carrier, such as a stabilizer, a dissolving agent, a vitamin, a pigment and a fragrance.
  • a typical adjuvant and carrier such as a stabilizer, a dissolving agent, a vitamin, a pigment and a fragrance.
  • a carrier such as an animal fiber, a plant fiber, a wax, paraffin, starch, tragacanth, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used.
  • a carrier such as lactose, talc, silica, aluminum hydroxide, calcium silicate or a polyamide powder may be used.
  • a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether may be further added.
  • a carrier such as a solvent, a solvating agent, or an emulsifying agent may be used, examples of which include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester.
  • a carrier such as a liquid diluent, such as water, ethanol or propylene glycol
  • a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ether or polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, may be used.
  • a carrier such as aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkyl amidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivatives or ethoxylated glycerol fatty acid ester may be used.
  • a Lactobacillus gasseri SKB1102 strain can exhibit complex functions, including an anti-pollution effect against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals, a skin irritation mitigation effect, a skin cell proliferation effect or a skin moisturizing effect, and thus the strain, a culture broth thereof or an extract thereof can be easily used for a cosmetic composition for a cosmeceutical product for improving the condition of the skin.
  • Korean Patent Application Publication No. 10-2014-0128675 discloses the anti-wrinkling and moisturizing effects of the Lactobacillus gasseri HY7025 strain, it is not stated or implied that the above strain has the same anti-pollution effect as the strain of the present invention.
  • FIG. 1 is a microscope image showing the morphology of Lactobacillus gasseri SKB1102 according to the present invention.
  • FIG. 2 is a phylogenetic tree showing the genetic position of Lactobacillus gasseri SKB1102 according to the present invention.
  • a SKB1102 strain having the greatest relative activity was selected from among strains having effects of removing a fine-dust-causing substance (removal), regenerating damaged skin (regeneration) and defending regenerated skin (defense) (the activity of this strain is disclosed in Examples 2 to 6, and the results of comparison thereof with other strains confirmed in the selection process are shown in Example 7).
  • phylogenetic analysis was performed by comparing 16S rRNA gene sequences. Using a Blast similarity search program (National Institute of Biotechnology Information), the base sequence of 16S rRNA (SEQ ID NO: 1) of the SKB1102 strain was compared with that of 16S rRNA of the standard Lactobacillus gasseri strain ATCC 33323. Consequently, these two strains were found to exhibit high homology of 99%. Also, the selected SKB1102 strain was subjected to multiple sequence alignment together with the 16S rRNA gene sequences of strains of the same type registered in GenBank, and the genetic position thereof in a phylogenetic tree was determined. The 16S rRNA gene of the SKB1102 strain in the phylogenetic tree was located at the position closest to Lactobacillus gasseri
  • the characteristics of the Lactobacillus gasseri SKB1102 are as follows. In order to confirm the morphological characteristics of the strain, culture was carried out for 1 day at 37°C on an MRS agar plate medium, whereby it was ascertained that the cells had streptobacillus morphology and no motility. The ability of the strain to form spores was not confirmed, and it is a gram-positive strain. Furthermore, the strain colonies were formed to be translucent, grayish, circular, and smooth. As for the physiological characteristics of the strain, it may be cultured at 25 to 40°C, and the optimal growth temperature thereof is 37°C. The tolerable pH ranges from 4.0 to 6.5, and the pH for optimal growth falls in the range of 6.0 to 6.5. The optimal growth time is 18 hr, and culture may be performed for a time ranging from 16 hours to 2 days. The above strain is a facultative anaerobic strain and generates no gas.
  • Lactobacillus gasseri SKB1102 was seeded in an MRS liquid medium (pH 4.5) and cultured at 37°C and 150 rpm for 30 hr, after which a culture broth having an absorbance of 5.5 at 600 nm was centrifuged at 3000 rpm for 10 min, and a cell-free supernatant was isolated and used for subsequent test samples.
  • MRS liquid medium pH 4.5
  • Human fibroblasts (ATCC) were seeded in a 24-well cell culture plate and then cultured for 1 day (37°C, 5% CO 2 ), after which the medium was replaced with a serum-free medium. After 24 hr, the supernatant sample of the culture broth was treated at different concentrations and cultured in a 5% CO 2 incubator at 37°C for 24 hr. After completion of the culture, the cells were reacted with a medium in which a 2.5 mg/ml MTT solution was diluted 10-fold, after which the medium was removed. Next, the MTT formazan crystal thus produced was dissolved in DMSO, and the absorbance thereof was measured at 540 nm. The results are shown in Table 1 below.
  • Immortalized human keratinocytes were seeded at 6 ⁇ 10 5 in a 6-well plate and cultured at 37°C for 24 hr in 5% CO 2 . After 24 hr, washing was performed with HBSS, the medium was replaced with a serum-free medium containing 10 ⁇ g/ml PM2.5 (a standard material such as fine dust or yellow dust having a size of about 2.5 ⁇ m collected from air), and the sample (the same as in Example 2) was treated at different concentrations (1%, 2%, and 2.5%) and cultured for 1 day.
  • PM2.5 a standard material such as fine dust or yellow dust having a size of about 2.5 ⁇ m collected from air
  • CYP1A1, interleukin-8, and interleukin-1 ⁇ are various factors for decreasing the effects of pollution resulting from increased sensitivity of skin cells due to irritation by, for example, fine dust, etc.
  • CYP1A1 mRNA expression of Table 4 upon treatment with 1.0 to 2.5 (v/v)% Lactobacillus gasseri SKB1102, a sensitivity reduction effect of 30.88% similar to that of a positive control was exhibited at 2.5 (v/v)%.
  • Lactobacillus gasseri SKB1102 of the present invention can be concluded to have the skin irritation mitigation effect.
  • keratinocytes Normal human keratinocytes (Gibco) were cultured in a 60 mm culture dish for 1 day (37°C, 5% CO 2 ), after which the medium was replaced with a serum-free medium. After 24 hr, the sample (the same as in Example 2) was treated at different concentrations and cultured in a 5% CO 2 incubator at 37°C for 24 hr. After completion of the culture, the cells were collected with TRIzol, RNA was isolated in accordance with the manufacturer's protocol, the isolated RNA was quantified, cDNA was synthesized from 1 ⁇ g of RNA, and real-time PCR was performed on moisturizing factors aquaporin-3 and CD-44 using the primers of Table 7 below. The results are shown in Tables 8 and 9 below.
  • Human fibroblasts were cultured in a 60 mm culture dish for 1 day (37°C, 5% CO 2 ), after which the medium was replaced with a serum-free medium. After 24 hr, the sample (the same as in Example 2) was treated at different concentrations and cultured in a 5% CO 2 incubator at 37°C for 24 hr. After completion of the culture, the cells were collected with TRIzol, RNA was isolated, the isolated RNA was quantified, cDNA was synthesized from 1 ⁇ g of RNA, and real-time PCR was performed on a skin-elasticity-improving or anti-wrinkling factor collagen-I using the primers of Table 10 below. The results are shown in Table 11 below.
  • Strain 3-in-1 anti-pollution cascade effects Strain Sensitivity mitigation effect(Relative activity, %) Moisturizing effect(Relative activity, %) Elasticity effect(Relative activity, %) SKB1093 55 64 60 SKB1094 ⁇ 50 53 ⁇ 50 SKB1211 58 67 76 SKB1233 70 ⁇ 50 66 SKB1192 64 58 82 SKB1234 55 77 76 SKB1281 78 ⁇ 50 69 SKB1212 82 78 80 SKB2031 67 72 82 SKB1121 59 ⁇ 50 54 SKB1081 78 66 ⁇ 50 SKB4031 69 67 77 SKB1213 ⁇ 50 58 65 SKB3041 66 78 66 SKB3031 82 ⁇ 50 88 SKB1261 76 69 58 SKB1021 69 80 ⁇ 50 SKB3021 ⁇ 50 82 78 SKB1291 79 54 72 SKB1262 67 72 89 SKB3032
  • a skin softener (skin, 100 g) containing a culture broth of Lactobacillus gasseri SKB1102 (sterilized after removing cells) was prepared through a typical method using ingredients in the amounts shown in Table 13 below.
  • a nourishing lotion (100 g) containing a concentrate obtained by extracting the cells of Lactobacillus gasseri SKB1102 using a 70 (v/v)% ethanol aqueous solution and then removing the solvent was prepared through a typical method using ingredients in the amounts shown in Table 14 below.
  • Ingredient Amount Concentrate of Lactobacillus gasseri SKB1102 cells 1.0 Sitosterol 1.7 Polyglyceryl 2-oleate 1.5 Ceteareth 1.2 Cholesterol 1.5 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower oil 10.0 Carboxyvinyl polymer 0.2 Xanthan gum 0.3 Preservative Trace Fragrance Trace Purified water Remainder
  • a nourishing cream (100 g) containing a culture broth of Lactobacillus gasseri SKB1102 (sterilized after removing cells) was prepared through a typical method using ingredients in the amounts shown in Table 15 below.
  • a foundation (100 g) containing a culture broth of Lactobacillus gasseri SKB1102 (sterilized after removing cells) was prepared through a typical method using ingredients in the amounts shown in Table 17 below.
  • a hair shampoo (100 g) containing a culture broth of Lactobacillus gasseri SKB1102 (sterilized after removing cells) was prepared through a typical method using ingredients in the amounts shown in Table 18 below.

Abstract

Disclosed is a Lactobacillus gasseri SKB1102 strain, which exhibits complex functions, including an anti-pollution effect against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals, a skin irritation mitigation effect, a skin cell proliferation effect or a skin moisturizing effect, whereby the strain, a culture broth thereof or an extract thereof can be easily used for a cosmetic composition for improving the condition of the skin.

Description

NOVEL LACTOBACILLUS GASSERI SKB1102 STRAIN OR COSMETIC COMPOSITION CONTAINING SAME HAVING ANTI-POLLUTION FUNCTION
The present invention relates to a Lactobacillus gasseri SKB1102 strain having an anti-pollution function against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals, or to a cosmetic composition containing the same. In addition, the present invention relates to the above strain or a cosmetic composition having a skin irritation mitigation effect, a skin cell proliferation effect or a skin moisturizing effect containing the same.
The epidermis of the skin is a barrier for preventing the penetration of chemicals and the evaporation of water in the body. The stratum corneum contains 10 to 25% water and the epidermal layer contains 70% water. The pH of the skin shows slight acidity of 5.0 to 6.0 on the surface, but the pH of the dermal layer in the skin is neutral, similar to that of body fluids. Meanwhile, when the water content of the stratum corneum falls below 10% due to skin drying in winter or upon frequent cleansing with soap, or due to skin aging attributable to age and health, skin elasticity and glossiness may decrease and fine wrinkling and skin cracking may occur. Furthermore, the human skin is exposed to fine dust and ultraviolet rays, thereby causing aging due to skin wrinkles, reduced elasticity, pigmentation, and the like, and damage to the skin barrier may result in decreased water content, keratinization, psoriasis and acne.
Fine dust or ultrafine dust, which is one of the main causes of skin damage that has recently received attention, is a kind of particulate matter (PM) having a diameter of 2.5 to 10 μm or having a diameter of 2.5 μm or less, and directly impedes the skin barrier function and aggravates dermatitis. Since the particle size of fine dust is very small, it may penetrate into the skin through hair follicles, and thus may affect other persons, in addition to patients with atopic and allergic diseases or with sensitive skin. When fine dust penetrates into the skin, it produces reactive oxygen species, which damages the mitochondria of the human skin, thus reducing collagen synthesis, accelerating degradation, and stimulating melanocytes to induce pigmentation, whereby skin aging, such as pigmentation, wrinkling and pore enlargement, may be aggravated.
Fine dust generates reactive oxygen species when penetrating into the skin to thus affect the skin, and thus, in order to protect the skin from fine dust, it is necessary to use basic cosmetics containing antioxidants to reduce the production of reactive oxygen species and prevent the effects thereof. Moreover, since fine dust itself may reduce the skin barrier function, it may be helpful to consume plenty of water and make use of moisturizers.
The term "cosmeceutical" refers to a product in which a cosmetic ingredient and a medicinal ingredient are combined, and has been used by cosmetic professionals since the 1990s. Dr. Albert Kligman started the development of cosmeceuticals. Dr. Kligman discovered that retinoid, which is a compound having a vitamin A skeleton, is very effective for restoring the human skin. Cosmeceutical products are used to play a role as functional cosmetics through the use of a filler, which is a plastic implant, or medically proven functional components for whitening, moisturizing, etc. These are mainly sold in types specialized for particular areas, such as serum, cream, oil, lip balm, and essence, which have the effects of sebum secretion control of acne-prone skin, keratin care, skin barrier enhancement function, and the like.
Examples of microorganisms serving as a material for a cosmeceutical product include lactic-acid bacteria. In general, lactic-acid bacteria exhibit immune enhancement effects in the human body through interactions with immune cells present in the intestinal mucosa after oral ingestion. Most lactic-acid bacteria useful as probiotics are gram-positive bacteria, in which cell walls exist outside the cell membrane and the cell walls have a complex layer structure of peptidoglycan, lipid protein, and teichoic acid. These cell wall components bind to MAMP by the PRR (pattern recognition receptor) expressed in the intestinal mucosa of humans to thus cause an immune response. The receptor that mediates the interaction with external bacterial flora is present in the skin as well as the intestinal mucosa, and thus lactic-acid bacteria are used in some skin cosmetics. However, in order to use a lactic-acid bacterium as a cosmetic ingredient for a cosmeceutical product, there is a need to scientifically prove the effects thereof, but it is merely applied to the extent necessary for fermented cosmetics or probiotic-fermented liquids, and is simply used for only a single product effect. Hence, required is a formulation having a triple effect which stepwisely solves skin problems depending on the condition of damaged skin.
The annual arrival of yellow dust and the phenomenon of fine dust, which is an air pollutant attributable to industrialization, lead to a skin aging cascade, including increasing the sensitivity of the exposed skin (step 1), decreasing moisturizing capability due to the damage to the skin barrier (step 2), and decreasing elasticity due to reduced skin water content (step 3).
Therefore, the present inventors analyzed the effects of fine dust, which causes the acceleration of skin sensitivity, on the skin barrier, and have developed a 3-in-1 formulation as a cosmeceutical material having all of anti-pollution cascade effects of fine-dust removal, skin moisturizing and skin elasticity when applied to the skin layer, using Lactobacillus gasseri SKB1102, which is a probiotic, thus culminating in the present invention.
[Citation List]
[Patent Literature]
(Patent Document 0001) Korean Patent Application Publication No. 10-2014-0128675 (Title: Probiotics of Lactobacillus gasseri HY7025 having skin wrinkle inhibitory and moisturizing effects and product containing the same as active ingredient, Applicant: Korea Yakult Co. Ltd., Laid-open Date: November 06, 2014)
(Patent Document 0002) Korean Patent Application Publication No. 10-2016-0038864 (Title: Method of producing microbial agent comprising intracellular accumulated aglycon therein and microbial agent produced thereby, Applicant: RNA Inc., Laid-open Date: April 07, 2016)
(Patent Document 0003) Korean Patent Application Publication No. 10-2010-0054428 (Title: Extract for improving glucose homeostasis fermented using Lactobacillus gasseri KCTC 3163 from ginseng or ginseng extract and method of preparing the same, Applicant: Korea Food Research Institute, Laid-open Date: May 25, 2010)
(Patent Document 0004) Korean Patent No. 10-1614141 (Title: Method of increasing yield of fermented ginseng seed oil, Applicant: Korea Food Research Institute, Registration Date: April 14, 2016)
Accordingly, an objective of the present invention is to provide a Lactobacillus gasseri SKB1102 strain having an anti-pollution function against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals, or a cosmetic composition containing the same. In addition, an objective of the present invention is to provide the above strain or a cosmetic composition having a skin irritation mitigation effect, a skin cell proliferation effect or a skin moisturizing effect containing the same.
The present invention provides a Lactobacillus gasseri SKB1102 strain having an anti-pollution function.
The strain has an anti-pollution function against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals contained in the air.
The strain may have a skin irritation mitigation effect.
The strain may have an effect of inhibiting the expression of a gene selected from the group consisting of CYP1A1 (Cytochrome P450, family 1, member A1), interleukin-8 and interleukin-1β, increased by an environmentally harmful factor.
The strain may have an effect of proliferation of at least one skin cell selected from among a human fibroblast and a human keratinocyte.
The strain may have a skin moisturizing effect.
The strain may increase the expression of a moisturizing factor selected from among aquaporin-3 and CD-44.
The strain may have a skin elasticity improvement effect.
The strain may increase the production of collagen type I, which is a skin-elasticity-related factor.
In addition, the present invention provides a cosmetic composition having an anti-pollution function, a skin irritation mitigation effect, a skin cell proliferation effect or a skin moisturizing effect, the cosmetic composition comprising cells of the Lactobacillus gasseri SKB1102 strain, a culture broth thereof, or an extract thereof.
The cosmetic composition may be provided in the form of a formulation selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milky lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream, a foundation, an essence, a nourishing essence, a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.
Hereinafter, a detailed description will be given of the present invention.
The Lactobacillus gasseri SKB1102 strain of the present invention may be cultured in an MRS agar medium or an MRS liquid medium at 25 to 40℃ and a pH of 4.0 to 6.5 for 16 hours to 2 days.
The cosmetic composition of the present invention may contain cells of a Lactobacillus gasseri SKB1102 strain, a culture broth including the cells, a culture broth excluding the cells, or an extract thereof, and the extract may be obtained by extracting an extraction sample using an extraction solvent such as water, a C1 to C4 lower alcohol, propylene glycol, butylene glycol, glycerin or a mixture thereof. The C1 to C4 lower alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol.
The extraction solvent used in the preparation of the extract may be used in a volume corresponding to 1 to 40 times (1 to 40 L relative to 1 kg) and preferably 5 to 40 times relative to the weight of a mixture. The extraction process for obtaining the extract may be performed at 20 to 100℃ for 1 min to 48 hr. This procedure may be repeated 1 to 4 times.
Also, in accordance with a typical method in the art, the extract may be dissolved in water and then additionally fractionated using at least one solvent selected from the group consisting of n-hexane, methylene chloride, acetone, chloroform, ethyl acetate and n-butanol, thus affording a fraction. Furthermore, typically known methods for use in the separation and extraction of plant components, such as extraction with an organic solvent (alcohol, ether, acetone, hexane, methylene chloride, ethyl acetate, etc.), distribution of an organic solvent such as hexane or butanol and water, column chromatography, and the like, may be used alone or in a suitable combination so as to realize fractionation or purification.
The temperature required to prepare the extract may fall in the range of 20 to 100℃, but the present invention is not limited thereto. The extraction time is not particularly limited, but preferably falls within the range of 10 minutes to 2 days. As the extraction device, a typical extraction device, an ultrasonication extractor or a fractionator may be used.
Upon preparation of the extract, when the extract solution is filtered, the filtration, which is a process of removing suspended solid particles from the extract solution, may be performed by filtering particles using cotton, nylon, paper or the like, or through ultrafiltration, freeze filtration, centrifugation or the like, but the present invention is not limited thereto.
The extraction process for preparing the extract is not particularly limited, and for example, cold extraction, ultrasonic extraction, reflux cooling extraction, room-temperature extraction, hot-water extraction and the like may be used depending on the kind of extract and the type of extraction process.
The extract thus prepared may be dried through hot-air drying, drying under reduced pressure, freeze drying, vacuum drying, spray drying, foam drying, high-frequency drying, infrared drying and the like, but the present invention is not limited thereto. In some cases, a process of grinding the final dried extract may be additionally performed.
The extract may be used after purification through column chromatography. The chromatography may be selected from among silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, medium-pressure liquid chromatography, thin-layer chromatography (TLC), silica gel vacuum liquid chromatography and high-performance liquid chromatography.
The strain of the present invention or the cosmetic composition containing the same has anti-pollution efficacy against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals contained in the air. Here, heavy metals are metals having a specific gravity of 4 or more, and may have a maximum specific gravity of 23, including mercury (13.5), cadmium (8.7), lead (11.3), copper (8.9) and the like.
In addition, the present invention addresses a cosmetic composition containing the Lactobacillus gasseri SKB1102, and the composition may contain 0.1 to 20 wt% of a Lactobacillus gasseri SKB1102 strain, a culture broth thereof, or an extract thereof.
The formulation of the cosmetic composition is not particularly limited, and is preferably selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milky lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream, a foundation, an essence, a nourishing essence, a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.
The cosmetic composition of the present invention may further contain a component selected from the group consisting of a water-soluble vitamin, an oil-soluble vitamin, a high-molecular-weight peptide, a high-molecular-weight polysaccharide, a sphingolipid and a seaweed extract.
As the water-soluble vitamin, any water-soluble vitamin may be used so long as it is capable of being blended in cosmetics, and preferable examples thereof include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C and vitamin H, and salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate, etc.) thereof are also included in the water-soluble vitamin that may be used in the present invention. The water-soluble vitamin may be obtained using a typical method such as a microbial transformation method, a method of purification from a microbial culture broth, an enzymatic method, or a chemical synthesis method.
As the oil-soluble vitamin, any oil-soluble vitamin may be used so long as it is capable of being blended in cosmetics, and preferable examples thereof include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (DL-α tocopherol, D-α tocopherol) and the like, and derivatives (ascorbyl palmitate, ascorbyl stearate, ascorbyl dipalmitate, DL-α tocopherol acetate, DL-α tocopherol nicotinate vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) thereof are also included in the oil-soluble vitamin that may be used in the present invention. The oil-soluble vitamin may be obtained using a typical method such as a microbial transformation method, a method of purification from a microbial culture broth, an enzymatic method, or a chemical synthesis method.
As the high-molecular-weight peptide, any high-molecular-weight peptide may be used so long as it is capable of being blended in cosmetics, and preferable examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like. The high-molecular-weight peptide may be obtained through purification using a typical method such as a method of purification from a microbial culture broth, an enzymatic method, or a chemical synthesis method, or may be used after purification from natural products such as the dermis of pigs and cows, the silk fiber of silkworms, etc.
As the high-molecular-weight polysaccharide, any high-molecular-weight polysaccharide may be used so long as it is capable of being blended in the cosmetic composition, and preferable examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or salts thereof (sodium salt, etc.) and the like. For example, chondroitin sulfate or salts thereof may be typically used after purification from mammals or fish.
As the sphingolipid, any sphingolipid may be used so long as it is capable of being blended in the cosmetic composition, and preferable examples thereof include ceramide, phytosphingosine and sphingoglycolipid. The sphingolipid may be obtained from mammals, fish, shellfish, yeast or plants through purification using a typical method or through chemical synthesis.
As the seaweed extract, any seaweed extract may be used so long as it is capable of being blended in the cosmetic composition, and preferable examples thereof include a brown algae extract, a red algae extract, a green algae extract, and the like, and also, carrageenan, alginic acid, sodium alginate, potassium alginate and the like, purified from the seaweed extract, are also included in the seaweed extract that may be used in the present invention. The seaweed extract may be obtained from seaweed through purification using a typical method.
The cosmetic composition of the present invention may also contain additional components that are blended in typical cosmetics.
Examples of the additionally blended components may include fat and oil components, a moisturizer, an emollient, a surfactant, organic and inorganic pigments, an organic powder, a UV absorber, a preservative, a disinfectant, an antioxidant, a plant extract, a pH adjuster, an alcohol, a colorant, a fragrance, a blood circulation accelerator, a cooling agent, an antiperspirant, purified water and the like.
Examples of the fat and oil components may include ester-based fats and oils, hydrocarbon-based fats and oils, silicone-based fats and oils, fluorine-based fats and oils, animal fats and oils, vegetable fats and oils, etc.
Examples of the ester-based fats and oils may include esters, such as glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diisopropyl adipate, isoalkyl neopentanoate, glyceryl tri(caprylate, caprate), trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, pentaerythritol tetra-2-ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostearyl laurate, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol di(caprylate, caprate), propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyl decyl isostearate, polyglycerin oleate ester, polyglycerin isostearate ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyl decyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl maleate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, isocetyl 12-stearoyl hydroxystearate, stearyl 12-stearoyl hydroxystearate, isostearyl 12-stearoyl hydroxystearate, and the like.
Examples of the hydrocarbon-based fats and oils may include squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, Vaseline, and the like.
Examples of the silicone-based fats and oils may include polymethylsilicone, methylphenylsilicone, methyl cyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethyl cyclosiloxane, dimethyl siloxaneㆍmethyl cetyloxysiloxane copolymer, dimethyl siloxaneㆍmethyl stearoxysiloxane copolymer, alkyl-modified silicone oil, amino-modified silicone oil, and the like.
Examples of the fluorine-based fats and oils may include perfluoropolyether and the like.
Examples of the animal or vegetable fats and oils may include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, safflower oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, grapeseed oil, cottonseed oil, coconut oil, kukui nut oil, wheat germ oil, rice germ oil, shea butter, evening primrose oil, macadamia　nut oil,　meadowfoam seed oil, egg yolk oil, beef tallow, horse oil, mink oil, orange roughy oil, jojoba oil, candelilla wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.
Examples of the moisturizer may include a water-soluble low-molecular-weight moisturizer, a fat-soluble low-molecular-weight moisturizer, a water-soluble high-molecular-weight moisturizer, a fat-soluble high-molecular-weight moisturizer, and the like.
Examples of the water-soluble low-molecular-weight moisturizer may include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n=2 or more), polypropylene glycol (polymerization degree n=2 or more), polyglycerin B (polymerization degree n=2 or more), lactic acid, lactate, and the like.
Examples of the fat-soluble low-molecular-weight moisturizer may include cholesterol, cholesterol ester, and the like.
Examples of the water-soluble high-molecular-weight moisturizer may include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, dextrin, and the like.
Examples of the fat-soluble high-molecular-weight moisturizer may include polyvinylpyrrolidoneㆍeicosene copolymer, polyvinylpyrrolidoneㆍhexadecene copolymer, nitrocellulose, dextrin fatty acid ester, high-molecular-weight silicone, and the like.
Examples of the emollient may include long-chain acylglutamate cholesteryl ester, cholesteryl hydroxystearate, 12-hydroxystearic acid, stearic acid, rhodinic acid, lanolin fatty acid cholesteryl ester, and the like.
Examples of the surfactant may include a nonionic surfactant, an anionic surfactant, a cationic surfactant, and an amphoteric surfactant.
Examples of the nonionic surfactant may include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitol fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POEㆍPOP (polyoxyethyleneㆍpolyoxypropylene) copolymer, POEㆍPOP alkyl ether, polyether-modified silicone, alkanolamide laurate, alkylamine oxide, hydrogenated soybean phospholipid, and the like.
Examples of the anionic surfactant may include fatty acid soap, α-acyl sulfonate, alkyl sulfonate, alkylallyl sulfonate, alkylnaphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkylamide phosphate, alkyloylalkyltaurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphoric acid ester, and the like.
Examples of the cationic surfactant may include alkyl trimethylammonium chloride, stearyl trimethylammonium chloride, stearyl trimethylammonium bromide, cetostearyl trimethylammonium chloride, distearyl dimethylammonium chloride, stearyl dimethylbenzylammonium chloride, behenyl trimethylammonium bromide, benzalkonium chloride, diethyl aminoethylamide stearate, dimethyl aminopropylamide stearate, lanolin-derived quaternary ammonium salts, and the like.
Examples of the amphoteric surfactant may include carboxybetaine-, amidobetaine-, sulfobetaine-, hydroxysulfobetaine-, amidosulfobetaine-, phosphobetaine-, aminocarboxylate-, imidazoline derivative- and amidoamine-type amphoteric surfactants.
Examples of the organic and inorganic pigments may include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, iron oxide red, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, carmine, and complexes thereof; organic pigments such as polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicone resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinyl benzeneㆍstyrene copolymer, silk powder, cellulose, CI pigment yellow and CI pigment orange; and combinations of these inorganic pigments and organic pigments.
Examples of the organic powder may include metal soap such as calcium stearate; alkylphosphoric acid metal salts such as sodium zinc cetylphosphate, zinc laurylphosphate and calcium laurylphosphate; acylamino acid polyvalent metal salts such as N-lauroyl-β-alanine calcium, N-lauroyl-β-alanine zinc and N-lauroylglycine calcium; amidosulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids such as N-e-lauroyl-L-lysine, N-e-palmitoyllysine, N-α-palmitoylornithine, N-α-lauroylarginine and N-α-hardened beef tallow fatty acid acylarginine; N-acylpolypeptides such as N-lauroylglycylglycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; polyethylene; polypropylene; nylon; polymethyl methacrylate; polystyrene; divinyl benzeneㆍstyrene copolymer; ethylene tetrafluoride, and the like.
Examples of the UV absorber may include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate, 2-ethoxyethyl para-methoxycinnamate, octyl para-methoxycinnamate, glyceryl mono-2-ethylhexanoate dipara-methoxycinnamate, isopropyl para-methoxycinnamate, diisopropyl diisopropylcinnamic acid ester mixtures, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxylmethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenonedisulfonate, dihydroxybenzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)-1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, and the like.
Examples of the disinfectant may include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc pyrithione, benzalkonium chloride, photosensitizing dye No. 301, sodium mononitroguaiacol, undecylenic acid, and the like.
Examples of the antioxidant may include butylhydroxyanisole, propyl gallate, erythorbic acid, and the like.
Examples of the pH adjuster may include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium hydrogen phosphate, and the like.
Examples of the alcohol may include higher alcohols such as cetyl alcohol and the like.
The additionally blended components are not limited thereto, and any of them may be used within a range that does not impair the purposes and effects of the present invention, but the amount thereof is preferably 0.01 to 5 wt %, and more preferably 0.01 to 3 wt%, based on the total weight of the cosmetic composition.
The cosmetic composition of the present invention may be provided in the form of a solution, an emulsion, or a viscous mixture.
The cosmetic composition of the present invention may contain, in addition to the extract as the active ingredient, components typically used in cosmetics, examples of which may include a typical adjuvant and carrier, such as a stabilizer, a dissolving agent, a vitamin, a pigment and a fragrance.
When the cosmetic composition of the present invention is formulated in the form of a paste, cream or gel, a carrier such as an animal fiber, a plant fiber, a wax, paraffin, starch, tragacanth, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used.
When the cosmetic composition of the present invention is formulated in the form of a powder or spray, a carrier such as lactose, talc, silica, aluminum hydroxide, calcium silicate or a polyamide powder may be used. In particular, when in a spray formulation, a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether may be further added.
When the cosmetic composition of the present invention is formulated in the form of a solution or an emulsion, a carrier such as a solvent, a solvating agent, or an emulsifying agent may be used, examples of which include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester.
When the cosmetic composition of the present invention is formulated in the form of a suspension, a carrier, such as a liquid diluent, such as water, ethanol or propylene glycol, a suspending agent, such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ether or polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, may be used.
When the cosmetic composition of the present invention is formulated in the form of a surfactant-containing cleanser, a carrier such as aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkyl amidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivatives or ethoxylated glycerol fatty acid ester may be used.
According to the present invention, a Lactobacillus gasseri SKB1102 strain can exhibit complex functions, including an anti-pollution effect against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals, a skin irritation mitigation effect, a skin cell proliferation effect or a skin moisturizing effect, and thus the strain, a culture broth thereof or an extract thereof can be easily used for a cosmetic composition for a cosmeceutical product for improving the condition of the skin. Although Korean Patent Application Publication No. 10-2014-0128675 discloses the anti-wrinkling and moisturizing effects of the Lactobacillus gasseri HY7025 strain, it is not stated or implied that the above strain has the same anti-pollution effect as the strain of the present invention.
FIG. 1 is a microscope image showing the morphology of Lactobacillus gasseri SKB1102 according to the present invention; and
FIG. 2 is a phylogenetic tree showing the genetic position of Lactobacillus gasseri SKB1102 according to the present invention.
A better understanding of preferred embodiments of the present invention will be given through the following examples. However, the present invention is not limited to these examples but may be embodied in other forms. These examples are provided to thoroughly explain the invention and to sufficiently transfer the spirit of the present invention to those skilled in the art.
<Example 1. Evaluation of genetic/physiological characteristics of strain>
In order to search for lactic-acid bacteria or culture products thereof applicable to cosmetic compositions having anti-pollution efficacy, a SKB1102 strain having the greatest relative activity was selected from among strains having effects of removing a fine-dust-causing substance (removal), regenerating damaged skin (regeneration) and defending regenerated skin (defense) (the activity of this strain is disclosed in Examples 2 to 6, and the results of comparison thereof with other strains confirmed in the selection process are shown in Example 7).
In order to identify the selected SKB1102 strain, phylogenetic analysis was performed by comparing 16S rRNA gene sequences. Using a Blast similarity search program (National Institute of Biotechnology Information), the base sequence of 16S rRNA (SEQ ID NO: 1) of the SKB1102 strain was compared with that of 16S rRNA of the standard Lactobacillus gasseri strain ATCC 33323. Consequently, these two strains were found to exhibit high homology of 99%. Also, the selected SKB1102 strain was subjected to multiple sequence alignment together with the 16S rRNA gene sequences of strains of the same type registered in GenBank, and the genetic position thereof in a phylogenetic tree was determined. The 16S rRNA gene of the SKB1102 strain in the phylogenetic tree was located at the position closest to Lactobacillus gasseri
Based on the results of molecular biological identification of the SKB1102 strain, this strain was classified as Lactobacillus gasseri. Thus, the above strain, having a triple anti-pollution action, was referred to as "Lactobacillus gasseri SKB1102" and was deposited with the Korean Culture Center of Microorganisms (KCCM, http://www.kccm.or.kr/) (Accession number: KCCM12342P).
Figure PCTKR2019007585-appb-I000001
Also, the characteristics of the Lactobacillus gasseri SKB1102 are as follows. In order to confirm the morphological characteristics of the strain, culture was carried out for 1 day at 37℃ on an MRS agar plate medium, whereby it was ascertained that the cells had streptobacillus morphology and no motility. The ability of the strain to form spores was not confirmed, and it is a gram-positive strain. Furthermore, the strain colonies were formed to be translucent, grayish, circular, and smooth. As for the physiological characteristics of the strain, it may be cultured at 25 to 40℃, and the optimal growth temperature thereof is 37℃. The tolerable pH ranges from 4.0 to 6.5, and the pH for optimal growth falls in the range of 6.0 to 6.5. The optimal growth time is 18 hr, and culture may be performed for a time ranging from 16 hours to 2 days. The above strain is a facultative anaerobic strain and generates no gas.
<Example 2. Evaluation of cytotoxicity of Lactobacillus gasseri SKB1102 on human fibroblasts>
Lactobacillus gasseri SKB1102 was seeded in an MRS liquid medium (pH 4.5) and cultured at 37℃ and 150 rpm for 30 hr, after which a culture broth having an absorbance of 5.5 at 600 nm was centrifuged at 3000 rpm for 10 min, and a cell-free supernatant was isolated and used for subsequent test samples.
Human fibroblasts (ATCC) were seeded in a 24-well cell culture plate and then cultured for 1 day (37℃, 5% CO2), after which the medium was replaced with a serum-free medium. After 24 hr, the supernatant sample of the culture broth was treated at different concentrations and cultured in a 5% CO2 incubator at 37℃ for 24 hr. After completion of the culture, the cells were reacted with a medium in which a 2.5 mg/ml MTT solution was diluted 10-fold, after which the medium was removed. Next, the MTT formazan crystal thus produced was dissolved in DMSO, and the absorbance thereof was measured at 540 nm. The results are shown in Table 1 below.
Cytotoxicity of Lactobacillus gasseri SKB1102 on human fibroblasts
Sample Concentration(%, v/v) Expression rate (%) St. dev. p-value
Control 100.00 4.48 1.0000
SKB1102 0.1 99.49 0.82 0.8553
0.5 107.46 2.01 0.0580
1.0 101.98 8.53 0.7465
1.5 101.52 3.54 0.6683
2.0 99.83 2.82 0.9581
3.0 111.45 2.27 0.0168
5.0 108.11 7.65 0.1882
As shown in the results of Table 1, when human fibroblasts were treated with 0.1 (v/v)% to 5.0 (v/v)% Lactobacillus gasseri SKB1102 and the cytotoxicity thereof was evaluated, toxicity was not apparent throughout the entire concentration range, and the fibroblasts were further grown at a concentration of 3.0 (v/v)% or more, from which cell regeneration effects can be confirmed to result.
<Example 3. Evaluation of cytotoxicity of Lactobacillus gasseri SKB1102 on human skin keratinocytes >
Normal human keratinocytes (Gibco) were seeded in a 24-well cell culture plate and cultured for 1 day (37℃, 5% CO2), after which the medium was replaced with a serum-free medium. After 24 hr, the sample (the same as in Example 2) was treated at different concentrations and cultured in a 5% CO2 incubator at 37℃ for 24 hr. After completion of the culture, the cells were reacted with a medium in which 2.5 mg/ml MTT solution was diluted 10-fold, after which the medium was removed. Next, the MTT formazan crystal thus produced was dissolved in DMSO, and the absorbance thereof was measured. The results are shown in Table 2 below.
Cytotoxicity of Lactobacillus gasseri SKB1102 on human keratinocytes
Sample Concentration(%, v/v) Expression rate (%) St. dev. p-value
Control - 100.00 0.64 1.0000
SKB1102 0.1 117.08 1.17 0.0000
0.5 117.58 5.85 0.0066
1.0 118.88 3.84 0.0011
1.5 114.79 3.19 0.0014
2.0 115.20 2.76 0.0008
3.0 112.28 1.43 0.0002
5.0 112.20 0.68 0.0000
As shown in the results of Table 2, when human keratinocytes were treated with 0.1 (v/v)% to 5.0 (v/v)% Lactobacillus gasseri SKB1102 and the cytotoxicity thereof was evaluated, toxicity was not apparent throughout the entire concentration range, and the human keratinocytes were grown in all treatment groups, from which skin regeneration effects can be confirmed to result in the keratinocytes.
<Example 4. Evaluation of effect of Lactobacillus gasseri SKB1102 on reducing sensitivity due to fine dust PM2.5>
Immortalized human keratinocytes were seeded at 6 × 105 in a 6-well plate and cultured at 37℃ for 24 hr in 5% CO2. After 24 hr, washing was performed with HBSS, the medium was replaced with a serum-free medium containing 10 μg/ml PM2.5 (a standard material such as fine dust or yellow dust having a size of about 2.5 μm collected from air), and the sample (the same as in Example 2) was treated at different concentrations (1%, 2%, and 2.5%) and cultured for 1 day. After completion of the culture, the cells were collected with TRIzol, RNA was isolated, the isolated RNA was quantified, cDNA was synthesized from 1 μg of RNA, and real-time PCR was performed using the primer sequences of Table 3 below. The results are shown in Tables 4 to 6 below.
Primer sequence for confirmation of mRNA expression of skin-sensitivity-related protein
Classification Base sequence
CYP1A1 Forward 5'- GGG ACC AGT CGG AAG GGA T-3'
Reverse 5'- CAC AGA TGG ACA GGC TGC CT-3'
IL-8 Forward 5'- CTG GCC GTG GCT CTC TTG-3'
Reverse 5'- CCT TGG CAA AAC TGC ACC TT-3'
IL-1β Forward 5'-ACG AAT CTC CGA CCA CCA CT-3'
Reverse 5'-TCC ATG GCC ACA ACA ACT GA-3'
β-Actin Forward 5'-GGC ACC CAG CAC AAT GAA G -3'
Reverse 5'-CCG ATC CAC ACG GAG TAC TTG -3'
Results of CYP1A1 mRNA expression
Sample Expression rate (%) St. dev. P-value
Control - 4.49 0.24 0.0000
PM 2.5 10 μg/ml 100.00 0.00 1.0000
PM 2.5 + Resveratrol 20 μM 67.67 0.44 0.0000
PM 2.5 + SKB1102 1.0(v/v)% 105.55 5.62 0.1624
2.0(v/v)% 99.84 2.16 0.9021
2.5(v/v)% 69.12 0.42 0.0000
Results of interleukin-8 (IL-8) mRNA expression
Sample Expression rate (%) St. dev. P-value
Control - 4.49 0.24 0.0000
PM 2.5 10 μg/ml 100.00 0.00 1.0000
PM 2.5 + Resveratrol 20 μM 67.67 0.44 0.0000
PM 2.5 + SKB1102 1.0(v/v)% 107.44 3.84 0.0285
2.0(v/v)% 99.76 3.87 0.0389
2.5(v/v)% 72.36 1.11 0.0001
Results of interleukin-1β mRNA expression
Sample Expression rate (%) St. dev. P-value
Control 46.73 2.02 0.0000
PM 2.5 10 μg/ml 100.00 0.00 1.0000
PM 2.5 + Resveratrol 20 μM 74.39 0.52 0.0000
PM 2.5 + SKB1102 1.0(v/v)% 105.29 1.82 0.0073
2.0(v/v)% 95.28 7.15 0.0674
2.5(v/v)% 80.26 12.02 0.1373
In Tables 4 to 6, CYP1A1, interleukin-8, and interleukin-1β are various factors for decreasing the effects of pollution resulting from increased sensitivity of skin cells due to irritation by, for example, fine dust, etc. With reference to the results of CYP1A1 mRNA expression of Table 4, upon treatment with 1.0 to 2.5 (v/v)% Lactobacillus gasseri SKB1102, a sensitivity reduction effect of 30.88% similar to that of a positive control was exhibited at 2.5 (v/v)%.
Also, with reference to the results of interleukin-8 mRNA expression of Table 5, upon treatment with 1.0 to 2.5 (v/v)% Lactobacillus gasseri SKB1102, a sensitivity reduction effect of 27.64% compared to a non-treated group was exhibited at 2.5 (v/v)%, and with reference to the results of interleukin-1β mRNA expression of Table 6, upon treatment with 1.0 to 2.5 (v/v)% Lactobacillus gasseri SKB1102, a sensitivity reduction effect of 19.74% compared to a non-treated group was exhibited at 2.5 (v/v)%.
Based on these results, Lactobacillus gasseri SKB1102 of the present invention can be concluded to have the skin irritation mitigation effect.
<Example 5. Evaluation of moisturizing effect of Lactobacillus gasseri SKB1102 >
Normal human keratinocytes (Gibco) were cultured in a 60 mm culture dish for 1 day (37℃, 5% CO2), after which the medium was replaced with a serum-free medium. After 24 hr, the sample (the same as in Example 2) was treated at different concentrations and cultured in a 5% CO2 incubator at 37℃ for 24 hr. After completion of the culture, the cells were collected with TRIzol, RNA was isolated in accordance with the manufacturer's protocol, the isolated RNA was quantified, cDNA was synthesized from 1 μg of RNA, and real-time PCR was performed on moisturizing factors aquaporin-3 and CD-44 using the primers of Table 7 below. The results are shown in Tables 8 and 9 below.
Primer sequence for confirmation of mRNA expression of moisturizing-related protein
Classification Base sequence
Aquaporin-3 Forward 5'- GGG ACC AGT CGG AAG GGA T-3'
Reverse 5'- CAC AGA TGG ACA GGC TGC CT-3'
CD-44 Forward 5'- GCT ATT GAA AGC CTT GCA GAG-3'
Reverse 5'- CGC AGA TCG ATT TGA ATA TAA CC-3'
β-Actin Forward 5'-GGC ACC CAG CAC AAT GAA G -3'
Reverse 5'-CCG ATC CAC ACG GAG TAC TTG -3'
Results of CD-44 mRNA expression
Sample Concentration(%, v/v) Expression rate (%) St. dev. p-value
Control 100.00 0.00 1.0000
SKB1102 1.0 104.92 7.13 0.29766
2.0 110.84 4.16 0.00117
2.5 114.86 8.25 0.00644
Retinoic acid 100 nM 138.40 12.35 0.00575
Results of Aquaporin-3 mRNA expression
Sample Concentration(%, v/v) Expression rate (%) St. dev. p-value
Control 100.00 0.00 1.0000
SKB1102 1.0 118.19 16.89 0.01587
2.0 124.76 16.93 0.01524
2.5 130.34 11.59 0.00109
Retinoic acid 100 nM 229.17 6.75 0.00005
As is apparent from the results of Table 8, the gene level involved in the expression of CD-44, which is present in keratinocytes and mediates a moisturizing effect, was increased by 14.86% upon treatment with 2.5 (v/v)% Lactobacillus gasseri SKB1102 compared to a non-treated group, thereby exhibiting superior moisturizing effects.
Also, as is apparent from the results of Table 9, the gene level involved in the expression of AQP-3, which is a protein associated with water molecule migration as a membrane pore protein, was increased by 30.34% upon treatment with 2.5 (v/v)% Lactobacillus gasseri SKB1102 compared to a non-treated group, thereby exhibiting outstanding moisturizing effects.
<Example 6. Evaluation of elasticity effect of Lactobacillus gasseri SKB1102 >
Human fibroblasts (ATCC) were cultured in a 60 mm culture dish for 1 day (37℃, 5% CO2), after which the medium was replaced with a serum-free medium. After 24 hr, the sample (the same as in Example 2) was treated at different concentrations and cultured in a 5% CO2 incubator at 37℃ for 24 hr. After completion of the culture, the cells were collected with TRIzol, RNA was isolated, the isolated RNA was quantified, cDNA was synthesized from 1 μg of RNA, and real-time PCR was performed on a skin-elasticity-improving or anti-wrinkling factor collagen-I using the primers of Table 10 below. The results are shown in Table 11 below.
Primer sequence for confirmation of mRNA expression of Collagen-I protein
Classification Base sequence
Collagen type I Forward 5'- AGC AAG AAC CCC AAG GAC AA-3'
Reverse 5'- CGA ACT GGA ATC CAT CGG TC-3'
β-Actin Forward 5'-GGC ACC CAG CAC AAT GAA G -3'
Reverse 5'-CCG ATC CAC ACG GAG TAC TTG -3'
Results of Collagen-I mRNA expression
Sample Concentration(%, v/v) Expression rate (%) St. dev. p-value
Control 100.00 0.00 1.0000
Vitamin C 100 μg/ml 123.11 5.42 0.0018
SKB1102 1.0 120.91 2.42 0.0000
2.0 128.95 3.08 0.0000
3.0 135.41 0.16 0.0001
As is apparent from Table 11, upon treatment with 3.0 (v/v)% Lactobacillus gasseri SKB1102, the mRNA expression rate of skin-elasticity-related collagen-I protein was confirmed to be significantly increased.
<Example 7. Efficacy of comparative strain confirmed during selection of Lactobacillus gasseri SKB1102>
Based on the results of Examples 2 to 6, the efficacy of each of lactic-acid bacteria compared and measured during the selection of Lactobacillus gasseri SKB1102 strain is shown in Table 12 below. Here, the results of Table 12 were represented as values relative to the efficacy of the Lactobacillus gasseri SKB1102 strain, which was set to 100%.
Strain 3-in-1 anti-pollution cascade effects
Strain Sensitivity mitigation effect(Relative activity, %) Moisturizing effect(Relative activity, %) Elasticity effect(Relative activity, %)
SKB1093 55 64 60
SKB1094 <50 53 <50
SKB1211 58 67 76
SKB1233 70 <50 66
SKB1192 64 58 82
SKB1234 55 77 76
SKB1281 78 <50 69
SKB1212 82 78 80
SKB2031 67 72 82
SKB1121 59 <50 54
SKB1081 78 66 <50
SKB4031 69 67 77
SKB1213 <50 58 65
SKB3041 66 78 66
SKB3031 82 <50 88
SKB1261 76 69 58
SKB1021 69 80 <50
SKB3021 <50 82 78
SKB1291 79 54 72
SKB1262 67 72 89
SKB3032 61 <50 56
SKB1101 82 72 78
SKB1235 89 64 <50
SKB1236 75 53 64
SKB1022 <50 67 78
SKB3022 58 82 76
SKB1263 64 <50 89
SKB5031 66 82 78
SKB4032 78 89 <50
SKB1082 <50 73 66
SKB4033 71 <50 82
SKB1083 <50 66 68
SKB1102 100 100 100
SKB5041 <50 62 <50
SKB1023 62 <50 88
SKB1095 88 77 75
SKB1237 85 82 80
SKB2032 74 89 80
SKB3033 <50 65 59
SKB5042 <50 <50 <50
<Cosmetic Formulation Example 1. Preparation of skin softener>
A skin softener (skin, 100 g) containing a culture broth of Lactobacillus gasseri SKB1102 (sterilized after removing cells) was prepared through a typical method using ingredients in the amounts shown in Table 13 below.
Ingredient Amount (g)
Culture broth of Lactobacillus gasseri SKB1102 3.0
Glycerin 3.0
Butylene glycol 2.0
Propylene glycol 2.0
Polyoxyethylene (60) Hardened castor oil 1.0
Ethanol 10.0
Triethanolamine 0.1
Preservative Trace
Colorant Trace
Fragrance Trace
Purified water Remainder
<Cosmetic Formulation Example 2. Preparation of nourishing lotion>
A nourishing lotion (100 g) containing a concentrate obtained by extracting the cells of Lactobacillus gasseri SKB1102 using a 70 (v/v)% ethanol aqueous solution and then removing the solvent was prepared through a typical method using ingredients in the amounts shown in Table 14 below.
Ingredient Amount (g)
Concentrate of Lactobacillus gasseri SKB1102 cells 1.0
Sitosterol 1.7
Polyglyceryl 2-oleate 1.5
Ceteareth 1.2
Cholesterol 1.5
Dicetyl phosphate 0.4
Concentrated glycerin 5.0
Sunflower oil 10.0
Carboxyvinyl polymer 0.2
Xanthan gum 0.3
Preservative Trace
Fragrance Trace
Purified water Remainder
<Cosmetic Formulation Example 3. Preparation of nourishing cream>
A nourishing cream (100 g) containing a culture broth of Lactobacillus gasseri SKB1102 (sterilized after removing cells) was prepared through a typical method using ingredients in the amounts shown in Table 15 below.
Ingredient Amount (g)
Culture broth of Lactobacillus gasseri SKB1102 5.0
Sitosterol 4.0
Polyglyceryl 2-oleate 3.0
Ceramide 0.7
Ceteareth-4 2.0
Cholesterol 3.0
Dicetyl phosphate 0.4
Concentrated glycerin 5.0
Sunflower oil 22.0
Carboxyvinyl polymer 0.5
Triethanolamine 0.5
Preservative Trace
Fragrance Trace
Purified water Remainder
<Cosmetic Formulation Example 4. Preparation of essence>
An essence (100 g) containing a culture broth of Lactobacillus gasseri SKB1102 (sterilized after removing cells) was prepared through a typical method using ingredients in the amounts shown in Table 16 below.
Ingredient Amount (g)
Culture broth of Lactobacillus gasseri SKB1102 1.0
Sitosterol 1.7
Polyglyceryl 2-oleate 1.5
Ceteareth-4 2.0
Cholesterol 3.0
Dicetyl phosphate 0.4
Concentrated glycerin 5.0
Sunflower oil 22.0
Carboxyvinyl polymer 0.5
Triethanolamine 0.5
Preservative Trace
Fragrance Trace
Purified water Remainder
<Cosmetic Formulation Example 5. Preparation of foundation>
A foundation (100 g) containing a culture broth of Lactobacillus gasseri SKB1102 (sterilized after removing cells) was prepared through a typical method using ingredients in the amounts shown in Table 17 below.
Ingredient Amount (g)
Culture broth of Lactobacillus gasseri SKB1102 1.0
Beeswax 2.0
Cyclomethicone 2.0
Liquid paraffin 5.0
Squalane 5.0
Stearic acid 2.0
Lipophilic glycerin monostearate 3.0
Caprylic/capric triglyceride 4.0
Glycerin 4.0
Propylene glycol 3.0
Butylene glycol 3.0
Triethanolamine 1.0
Aluminum magnesium silicate 0.5
Pigment 12.0
Preservative Trace
Fragrance Trace
Purified water Remainder
<Cosmetic Formulation Example 6. Preparation of hair shampoo>
A hair shampoo (100 g) containing a culture broth of Lactobacillus gasseri SKB1102 (sterilized after removing cells) was prepared through a typical method using ingredients in the amounts shown in Table 18 below.
Ingredient Amount (g)
Culture broth of Lactobacillus gasseri SKB1102 3.0
Arachidyl glucoside 4.5
Ethanol 2.0
Butylene glycol 2.0
Citric acid 0.1
Phenoxyethanol 0.02
Purified water Remainder
[Depositary Authority]
Name of Depositary Authority: Korean Culture Center of Microorganisms
Accession number : KCCM12342P
Accession date: 20181016
Figure PCTKR2019007585-appb-I000002

Claims (12)

  1. A Lactobacillus gasseri SKB1102 (Accession number: KCCM12342P) strain having an anti-pollution function.
  2. The Lactobacillus gasseri SKB1102 strain of claim 1, wherein the strain has an anti-pollution function against at least one environmentally harmful factor selected from the group consisting of fine dust, yellow dust, vehicle exhaust gas, dioxin, benzopyrene and heavy metals contained in air.
  3. The Lactobacillus gasseri SKB1102 strain of claim 1, wherein the strain has a skin irritation mitigation effect.
  4. The Lactobacillus gasseri SKB1102 strain of claim 1, wherein the strain has an effect of inhibiting expression of a gene selected from the group consisting of CYP1A1 (Cytochrome P450, family 1, member A1), interleukin-8 and interleukin-1β, increased by an environmentally harmful factor.
  5. The Lactobacillus gasseri SKB1102 strain of claim 1, wherein the strain has an effect of proliferation of at least one skin cell selected from among a human fibroblast and a human keratinocyte.
  6. The Lactobacillus gasseri SKB1102 strain of claim 1, wherein the strain has a skin moisturizing effect.
  7. The Lactobacillus gasseri SKB1102 strain of claim 1, wherein the strain increases expression of a moisturizing factor selected from among aquaporin-3 and CD-44.
  8. The Lactobacillus gasseri SKB1102 strain of claim 1, wherein the strain has a skin elasticity improvement effect.
  9. The Lactobacillus gasseri SKB1102 strain of claim 1, wherein the strain has a function of increasing production of collagen type I, which is a skin-elasticity-related factor.
  10. A cosmetic composition having an anti-pollution function, comprising a cell of the strain of claim 1, a culture broth thereof, or an extract thereof.
  11. The cosmetic composition of claim 10, wherein the composition has a skin irritation mitigation effect, a skin cell proliferation effect or a skin moisturizing effect.
  12. The cosmetic composition of claim 10 or 11, wherein the composition has a formulation selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milky lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream, a foundation, an essence, a nourishing essence, a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.
PCT/KR2019/007585 2018-11-09 2019-06-24 Novel lactobacillus gasseri skb1102 strain or cosmetic composition containing same having anti-pollution function WO2020096156A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2018-0137136 2018-11-09
KR1020180137136A KR101953072B1 (en) 2018-11-09 2018-11-09 Novel lactic acid bacteria Lactobacillus gasseri SKB1102, or Cosmetic composition comprising the same for anti-pollution

Publications (1)

Publication Number Publication Date
WO2020096156A1 true WO2020096156A1 (en) 2020-05-14

Family

ID=65560780

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2019/007585 WO2020096156A1 (en) 2018-11-09 2019-06-24 Novel lactobacillus gasseri skb1102 strain or cosmetic composition containing same having anti-pollution function

Country Status (2)

Country Link
KR (1) KR101953072B1 (en)
WO (1) WO2020096156A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111826312A (en) * 2020-07-10 2020-10-27 江南大学 Lactobacillus rhamnosus capable of relieving benzopyrene exposure and application thereof
CN114250167A (en) * 2020-09-24 2022-03-29 统欣生物科技股份有限公司 Lactobacillus gasseri strain, composition and application thereof
TWI779358B (en) * 2020-09-24 2022-10-01 統欣生物科技股份有限公司 Lactobacillus gasseri strain, composition and use thereof

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101953072B1 (en) * 2018-11-09 2019-02-27 에스케이바이오랜드 주식회사 Novel lactic acid bacteria Lactobacillus gasseri SKB1102, or Cosmetic composition comprising the same for anti-pollution
TWI815042B (en) * 2019-09-24 2023-09-11 大江生醫股份有限公司 Use of leuconostoc mesenteroides tci007 metabolite for improving allergy
KR102169163B1 (en) * 2019-10-24 2020-10-22 대한민국 Cosmetic composition comprising extract of Cyperus flavidus
KR102157970B1 (en) 2019-11-14 2020-09-21 에스케이바이오랜드 주식회사 Cosmetic composition containing lactic acid bacteria fermented extract of germinated sprout for skin barrier reinforcement
KR102486626B1 (en) 2020-10-07 2023-01-10 동의대학교 산학협력단 Skin improvement composition containing novel organic compounds as active ingredients and uses thereof
KR102499330B1 (en) 2022-04-26 2023-02-10 주식회사 현대바이오랜드 Cosmetic composition containing culture with complex strains separated from kombucha for skin barrier reinforcement
KR102552982B1 (en) 2022-08-22 2023-07-06 주식회사 현대바이오랜드 Cosmetic composition containing fermented extract of Perilla frutescens grown using a red LED light source for skin elasticity enhancement or inhibiting photoaging

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003253262A (en) * 2002-02-28 2003-09-10 Snow Brand Milk Prod Co Ltd Antioxidant
KR20130020737A (en) * 2005-03-03 2013-02-27 가부시키가이샤 메이지 Immune function modulating agents
KR20140128675A (en) * 2013-04-29 2014-11-06 주식회사한국야쿠르트 Probiotics of Lactobacillus gasseri HY7025 for skin wrinkle inhibitory and moisturizing effects and use of thereof as skin anti-wrinkle or moisturizing products
CN104188183A (en) * 2014-08-28 2014-12-10 胡安然 Mask for defending haze and preventing and treating sphagitis
KR20170049216A (en) * 2015-10-28 2017-05-10 고려대학교 산학협력단 Novel Lactobacillus gasseri and Uses Thereof
KR101953072B1 (en) * 2018-11-09 2019-02-27 에스케이바이오랜드 주식회사 Novel lactic acid bacteria Lactobacillus gasseri SKB1102, or Cosmetic composition comprising the same for anti-pollution

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100054428A (en) 2008-11-14 2010-05-25 한국식품연구원 Extracts for improving glucose homeostasis fermented using lactobacillus gasseri kctc 3163 from ginseng or ginseng extract
KR101614141B1 (en) 2014-01-14 2016-04-20 한국식품연구원 Method for increasing the yield of fermented ginseng seed oil
US10676708B2 (en) 2014-09-30 2020-06-09 Rna Inc. Method for preparing microbial preparation and microbial preparation produced by the same

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003253262A (en) * 2002-02-28 2003-09-10 Snow Brand Milk Prod Co Ltd Antioxidant
KR20130020737A (en) * 2005-03-03 2013-02-27 가부시키가이샤 메이지 Immune function modulating agents
KR20140128675A (en) * 2013-04-29 2014-11-06 주식회사한국야쿠르트 Probiotics of Lactobacillus gasseri HY7025 for skin wrinkle inhibitory and moisturizing effects and use of thereof as skin anti-wrinkle or moisturizing products
CN104188183A (en) * 2014-08-28 2014-12-10 胡安然 Mask for defending haze and preventing and treating sphagitis
KR20170049216A (en) * 2015-10-28 2017-05-10 고려대학교 산학협력단 Novel Lactobacillus gasseri and Uses Thereof
KR101953072B1 (en) * 2018-11-09 2019-02-27 에스케이바이오랜드 주식회사 Novel lactic acid bacteria Lactobacillus gasseri SKB1102, or Cosmetic composition comprising the same for anti-pollution

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111826312A (en) * 2020-07-10 2020-10-27 江南大学 Lactobacillus rhamnosus capable of relieving benzopyrene exposure and application thereof
CN114250167A (en) * 2020-09-24 2022-03-29 统欣生物科技股份有限公司 Lactobacillus gasseri strain, composition and application thereof
TWI779358B (en) * 2020-09-24 2022-10-01 統欣生物科技股份有限公司 Lactobacillus gasseri strain, composition and use thereof

Also Published As

Publication number Publication date
KR101953072B1 (en) 2019-02-27

Similar Documents

Publication Publication Date Title
WO2020096156A1 (en) Novel lactobacillus gasseri skb1102 strain or cosmetic composition containing same having anti-pollution function
US20220143112A1 (en) Novel bifidobacterium longum strain or cosmetic composition comprising same
WO2019004673A2 (en) Anti-aging composition comprising lactic acid bacteria-derived extracellular vesicle
KR102157970B1 (en) Cosmetic composition containing lactic acid bacteria fermented extract of germinated sprout for skin barrier reinforcement
WO2021006549A1 (en) Method for preparing dermobiotics block composition having skin moisturizing and skin regeneration functions
KR101881954B1 (en) Cosmetic composition containing gynostemma pentaphyllum leaf extract
WO2014069874A1 (en) Composition for removing keratinous skin material comprising green tea lactobacillus
WO2018062751A1 (en) Composition containing lysate of lactobacillus plantarum for hair or scalp
KR100773856B1 (en) Composition comprising an extract of siraitia grosvenori or triterpene compound isolated therefrom showing anti-wrinkle activity
KR20090043491A (en) Anti-wrinkle agent
WO2021125488A1 (en) Lipolysis composition using surface-modified gas-generating nanoparticles
WO2023027274A1 (en) Cosmetic composition containing iris germanica root-derived exosomes as active ingredient
KR20160111888A (en) Wheat seed extract showing potent inhibiting effect on skin aging, the preparation thereof and the compostion comprising the same
KR101764822B1 (en) Cosmetic composition for whitening skin color
KR102397879B1 (en) Cosmetic composition comprising extracts of burdock seed and Torreya grandis aril for improving wrinkles, elasticity or moisturizing the skin
WO2019035514A1 (en) Horse oil purification method and cosmetic composition using horse oil purified thereby
KR101191724B1 (en) Composition comprising a leaf extract of Isatis indigotica Fort showing skin whitening and anti-wrinkle activity
WO2019172554A1 (en) Skin-whitening cosmetic composition containing lactobacillus culture mixture solution
KR101849885B1 (en) Cosmetics comprising whey obtained by fermentation with Korean type lactic acid bacteria complex for improving elasticity or moisturizing
JP2002265377A (en) Physiologically active composition derived from rice bran
KR102284899B1 (en) Cosmetic composition comprising mature peptides of Astragalus membranaceus for improving skin vitality
KR102335297B1 (en) Cosmetic composition comprising Ultra-high pressure treated artichoke leaf, camellia leaf and caper fruit complex extract having effect relief itching caused by external stimulation or skin soothing effect
KR102091923B1 (en) Cosmetic composition comprising extract of Spirodela polyrhiza for improvement of skin damage or skin-protection
KR101912097B1 (en) Cosmetic composition comprising extract from curcuma and oenothera laciniata hill
KR100714172B1 (en) Cosmetic composition having the anti-wrinkle activity

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 19882925

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 19882925

Country of ref document: EP

Kind code of ref document: A1