WO2020088997A3 - Mikroskop und verfahren zur mikroskopie - Google Patents

Mikroskop und verfahren zur mikroskopie Download PDF

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Publication number
WO2020088997A3
WO2020088997A3 PCT/EP2019/078805 EP2019078805W WO2020088997A3 WO 2020088997 A3 WO2020088997 A3 WO 2020088997A3 EP 2019078805 W EP2019078805 W EP 2019078805W WO 2020088997 A3 WO2020088997 A3 WO 2020088997A3
Authority
WO
WIPO (PCT)
Prior art keywords
illumination
detector
sample
microscope
evaluation unit
Prior art date
Application number
PCT/EP2019/078805
Other languages
English (en)
French (fr)
Other versions
WO2020088997A2 (de
Inventor
Daniel Schwedt
Tiemo Anhut
Original Assignee
Carl Zeiss Microscopy Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carl Zeiss Microscopy Gmbh filed Critical Carl Zeiss Microscopy Gmbh
Priority to CN201980071523.6A priority Critical patent/CN113056696B/zh
Priority to US17/289,838 priority patent/US20210404964A1/en
Publication of WO2020088997A2 publication Critical patent/WO2020088997A2/de
Publication of WO2020088997A3 publication Critical patent/WO2020088997A3/de

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/004—Scanning details, e.g. scanning stages fixed arrays, e.g. switchable aperture arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/0044—Scanning details, e.g. scanning stages moving apertures, e.g. Nipkow disks, rotating lens arrays
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/0048—Scanning details, e.g. scanning stages scanning mirrors, e.g. rotating or galvanomirrors, MEMS mirrors
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0068—Optical details of the image generation arrangements using polarisation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0072—Optical details of the image generation details concerning resolution or correction, including general design of CSOM objectives
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/02—Objectives
    • G02B21/025—Objectives with variable magnification
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/0816—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light by means of one or more reflecting elements
    • G02B26/0833—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light by means of one or more reflecting elements the reflecting element being a micromechanical device, e.g. a MEMS mirror, DMD
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0938—Using specific optical elements
    • G02B27/0944—Diffractive optical elements, e.g. gratings, holograms
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/28—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising
    • G02B27/283—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising used for beam splitting or combining
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison

Abstract

Die Erfindung betrifft ein Mikroskop mit einer Lichtquelle zum Bereitstellen von Beleuchtungslicht, mit einer ansteuerbaren Manipulationseinrichtung zum variablen Erzeugen eines auszuwählenden Beleuchtungsmusters des Beleuchtungslichts, mit einem Beleuchtungsstrahlengang mit einem Mikroskopobjektiv zum Leiten des Beleuchtungsmusters auf eine zu untersuchende Probe, mit einem Detektor mit einer Vielzahl von Pixeln zum Nachweisen von von der Probe abgestrahltem Fluoreszenzlicht, mit einem Detektionsstrahlengang zum Leiten des von der Probe abgestrahlten Fluoreszenzlichts auf den Detektor, mit einem Hauptstrahlteiler zum Trennen von Beleuchtungslicht und Fluoreszenzlicht, mit einer Steuer- und Auswerteeinheit zum Ansteuern der Manipulationseinrichtung und zum Auswerten der von dem Detektor gemessenen Daten. Das erfindungsgemäße Mikroskop ist dadurch gekennzeichnet, dass die Manipulationseinrichtung im Beleuchtungsstrahlengang strahlaufwärts von dem Hauptstrahlteiler in der Nähe einer zu einer Probenebene optisch konjugierten Ebene angeordnet ist, dass die Pixel des Detektors mit der Steuer- und Auswerteeinheit einzeln und in auszuwählenden Auslesemustern aktivierbar sind und dass die Steuer- und Auswerteeinheit eingerichtet ist zum Aktivieren von Pixeln des Detektors einzeln oder in einem ausgewählten Auslesemuster abhängig von einem ausgewählten Beleuchtungsmuster. Die Erfindung betrifft außerdem ein Verfahren zur Mikroskopie.
PCT/EP2019/078805 2018-10-31 2019-10-23 Mikroskop und verfahren zur mikroskopie WO2020088997A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201980071523.6A CN113056696B (zh) 2018-10-31 2019-10-23 显微镜和显微镜检查的方法
US17/289,838 US20210404964A1 (en) 2018-10-31 2019-10-23 Microscope and method for microscopy

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102018127281.2 2018-10-31
DE102018127281.2A DE102018127281A1 (de) 2018-10-31 2018-10-31 Mikroskop und Verfahren zur Mikroskopie

Publications (2)

Publication Number Publication Date
WO2020088997A2 WO2020088997A2 (de) 2020-05-07
WO2020088997A3 true WO2020088997A3 (de) 2020-08-13

Family

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PCT/EP2019/078805 WO2020088997A2 (de) 2018-10-31 2019-10-23 Mikroskop und verfahren zur mikroskopie

Country Status (3)

Country Link
US (1) US20210404964A1 (de)
DE (1) DE102018127281A1 (de)
WO (1) WO2020088997A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220075171A1 (en) * 2019-01-25 2022-03-10 Carl Zeiss Microscopy Gmbh Light microscope with photon-counting detector elements and imaging method
DE102020113998A1 (de) 2020-05-26 2021-12-02 Abberior Instruments Gmbh Verfahren, Computerprogramm und Vorrichtung zum Bestimmen von Positionen von Molekülen in einer Probe
WO2024037711A1 (en) * 2022-08-17 2024-02-22 Ceramic Data Solutions GmbH Compact writing and reading head for hyper speed data recording on ceramic material

Citations (4)

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Publication number Priority date Publication date Assignee Title
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US6191885B1 (en) * 1998-09-22 2001-02-20 Olympus Optical Co., Ltd. Confocal microscope apparatus and photographing apparatus for confocal microscope
DE102013018672A1 (de) * 2013-11-07 2015-05-07 Carl Zeiss Microscopy Gmbh Multispot-scanning mikroskop
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ATE236412T1 (de) * 1997-10-22 2003-04-15 Max Planck Gesellschaft Programmierbares räumlich lichtmoduliertes mikroskop und mikroskopieverfahren
WO2004038461A2 (en) * 2002-10-22 2004-05-06 William Marsh Rice University Random access high-speed confocal microscope
DE102006047258A1 (de) * 2006-10-04 2008-04-10 Deutsches Zentrum für Luft- und Raumfahrt e.V. Verfahren und Vorrichtung zum Auslösen einer Aufzeichnung von Bildern
JP5852305B2 (ja) * 2009-10-28 2016-02-03 カール ツァイス マイクロスコピー ゲーエムベーハーＣａｒｌ Ｚｅｉｓｓ Ｍｉｃｒｏｓｃｏｐｙ Ｇｍｂｈ 分解能の向上した顕微鏡法および顕微鏡
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DE102015112960B3 (de) * 2015-08-06 2016-10-20 Till I.D. Gmbh Vorrichtung für die konfokale Beleuchtung einer Probe
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US6191885B1 (en) * 1998-09-22 2001-02-20 Olympus Optical Co., Ltd. Confocal microscope apparatus and photographing apparatus for confocal microscope
DE102013018672A1 (de) * 2013-11-07 2015-05-07 Carl Zeiss Microscopy Gmbh Multispot-scanning mikroskop
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Also Published As

Publication number Publication date
CN113056696A (zh) 2021-06-29
US20210404964A1 (en) 2021-12-30
DE102018127281A1 (de) 2020-04-30
WO2020088997A2 (de) 2020-05-07

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