WO2020067798A1 - Bio-cellulose sheet and preparation method thereof - Google Patents

Abstract

Provided are a bio-cellulose sheet which comprises a non-woven fabric and bio-cellulose and has a structure in which fibers of the non-woven fabric and bio-cellulose fibers are tangled, a preparation method thereof, and a cosmetic mask comprising the sheet.

Description

Biocellulose sheet and manufacturing method thereof

Cross-citation with relevant application (s)

This application claims the benefit of priority based on Korean Patent Application No. 10-2018-0115318 on September 27, 2018 and Korean Patent Application No. 10-2019-0119146 on September 26, 2019. All content disclosed in the literature is incorporated as part of this specification.

The present invention relates to a biocellulose sheet comprising a nonwoven fabric and biocellulose and having a structure in which fibers of the nonwoven fabric and biocellulose fibers are entangled, a method for manufacturing the same, and a cosmetic pack or medical patch comprising the sheet.

A sheet used in the cosmetic and medical field refers to a fabric that can be attached to the skin by supporting a cosmetic or medicinal component in the form of a liquid, cream or emulsion. Such a sheet can support a variety of efficacy ingredients, and when attached to the skin, the efficacy ingredients penetrate the skin and exhibit cosmetic or therapeutic effects. Therefore, such a sheet may be used as a cosmetic pack for moisturizing, whitening or nourishing the skin, or may be used as a medical patch such as for treatment of burns or wounds.

Sheets have been manufactured using a variety of materials. The sheet, which was initially developed, is a non-woven material, and a non-woven fabric blended with rayon and polypropylene is coated with cosmetics for the purpose of whitening, anti-wrinkle, and nutrition. Non-woven fabrics were easy to secure materials and cheap, so they were distributed in large quantities at a low price on the market. Subsequently, a cotton material was developed to replace the non-woven fabric, and instead of the synthetic blend rayon, natural cotton, pure cotton, was used to minimize skin irritation and improve absorbency and adhesion. In addition, a sheet of hydrogel material has been developed, which improves the disadvantages of the sheet of the existing nonwoven or cotton material in which the essence flows down when used, and delivers nutrients or active ingredients deep into the skin, thereby accelerating the growth of cosmetic packs. However, the hydrogel sheet has a problem that may cause skin problems due to some synthetic cross-linking agents.

Accordingly, the sheet that has recently been spotlighted is a biocellulose material, which uses natural biocellulose obtained by microbial culture. Virocellulose sheet is a natural material that has no side effects on the body, is eco-friendly, has excellent adhesion and fit, and has a moisture content of more than 10 times that of a conventional non-woven fabric or cotton material. There are advantages.

However, biocellulose is easily damaged (teared) due to its softer and softer texture than vegetable cellulose, and has a problem in handling. However, in order to reduce the breakage of the bio-cellulose sheet, when the bio-cellulose is thickly produced, it takes a long time, and due to the thick thickness, there is a limit to sufficiently supplying useful nutrients to the skin, and there is a problem that it cannot sufficiently cover the curved portion of the skin. .

Thus, in the case of the currently commercialized biocellulose sheet, in order to prevent tearing of the sheet and to facilitate handling, in order to fix the shape after manufacturing the biocellulose sheet, a support film is laminated or attached to one or both sides of the sheet. Is being provided. The supporting film includes a nonwoven fabric film, a reticulated fiber cloth film, a polymer thin film film, a waterproof film film, a polymer foam film, or a paper film, etc., and the user must remove it immediately before use.

However, when a supporting film is attached to the biocellulose sheet, an additional process of laminating the supporting film after manufacturing the biocellulose sheet is required, and the manufacturing cost increases due to the cost of the supporting film, and the user supports each time the product is used. There is a hassle of removing the film.

Accordingly, there is a need to develop an improved biocellulose sheet capable of solving the fragility of tearing and handling, while maintaining excellent adhesion, fit and absorbency, which are advantages of the biocellulose sheet.

As an improved biocellulose sheet, it provides a biocellulose sheet that is resistant to tearing and easy to handle without the use of a support film, and maintains the adhesion, fit and absorbency, which are advantages of the biocellulose sheet.

In one embodiment, a non-woven fabric and bio-cellulose, and provides a bio-cellulose sheet having a structure in which the fibers of the non-woven fabric and the bio-cellulose fibers are entangled.

Impregnating the bio-cellulose culture with a nonwoven fabric; And culturing the non-woven fabric impregnated with the culture solution.

It provides a cosmetic pack comprising a bio-cellulose sheet.

Provided is a medical patch comprising a biocellulose sheet.

It is easy to adhere, fit and absorbent, and is resistant to tearing of the sheet, easy to handle, and can be used immediately without the need for a separate support film. In addition, the manufacturing process is easy, and the use amount of the microbial culture solution is reduced, thereby reducing the manufacturing time and cost.

1 is a scanning electron microscope (SEM) photograph of a biocellulose sheet according to an embodiment, No. 1 shows a surface in which biocellulose is not formed in the biocellulose sheet and only nonwoven fibers exist, and number 2 and 3 are biocellulose sheets The bio-cellulose nano-sized fiber and the non-woven micro fiber show another surface in a tangled structure.

The left photograph of FIG. 2 is a SEM photograph of a cross-section of a biocellulose sheet having a non-woven fabric film laminated on both sides of the prior art, and it can be seen that the fiber of the bio-cellulose and the fiber of the non-woven fabric are not entangled. The photo on the right is a SEM image of a cross section of a biocellulose sheet according to an embodiment of the present application, and there is a structure in which nanofibers of biocellulose and microfibers of nonwoven fabric are entangled on one surface of the sheet.

According to one aspect of the present invention, there is provided a biocellulose sheet comprising a nonwoven fabric and biocellulose, and having a structure in which fibers of the nonwoven fabric and biocellulose fibers are entangled.

In general, cellulose is a basic structural component of a cell wall of a plant, and refers to a fiber constituting the plant fiber. For example, about 98% of the cotton wool is cellulose, and the fibers of flax or hemp contain about 70% cellulose. In contrast, biocellulose is a polymer polymer produced through microbial culture, and is also called microbial cellulose or bacterial cellulose.

Production strains of biocellulose include Acetobacter genus, Gluconacetobacter genus, Agrobacterium genus, Lactobacillus genus, Rhizobium genus, Pseudomonas genus , Or Sarsina genus strains are known. When these strains are cultured in a medium containing carbon and nitrogen, biocellulose in the form of a translucent membrane is produced at the interface of the culture medium. The biocellulose thus formed has a high mechanical strength and a three-dimensional network structure composed of extremely fine and pure cellulose.

In addition, biocellulose corresponds to a polymer in which glucose is combined with β-1,4, such as cellulose derived from plants, but unlike plant-derived cellulose, it is produced in a pure state without foreign substances such as lignin, hemicellulose, or pectin. Easy separation and purification. The network-like biocellulose coating is a randomly formed ribbon-shaped fibrillated cellulose, and its width is smaller than 100 nm and consists of a myriad of fine fibrils having a radius of 2 to 4 nm.

As described above, bio-cellulose has much less fiber width than plant cellulose, has a three-dimensional network structure, and has excellent water retention properties, and has a soft texture such as a hydrogel mask and a smooth fit. It is suitable for use in cosmetics and medical fields due to its nano-structural properties, moisture retention, excellent fit and biocompatibility.

Biocellulose sheet refers to a sheet made of biocellulose in a shape that can be cut to an appropriate thickness and / or form and attached to the body. It can be attached to the body by supporting liquid, cream or latex cosmetic or pharmaceutical ingredients. have. In the present specification, unless defined otherwise, the biocellulose sheet means only the sheet itself that does not include a support film that is detachably attached or laminated thereto.

The biocellulose sheet provided herein includes a nonwoven fabric and biocellulose, and is characterized by having a structure in which fibers of the nonwoven fabric and biocellulose fibers are entangled. Therefore, since the nonwoven fabric is included in the configuration of the biocellulose sheet itself, and has a structure in which the nonwoven fabric and the biocellulose fibers are entangled, it is a structure that is not detachable from each other. It is different from the non-woven film.

As used herein, "entanglement", "entangled structure", or "entangled structure" refers to a state in which non-woven fibers and biocellulose fibers are entangled like noodles, in which case the two fibers are separated or separated from each other. Because of this, the nonwoven fabric and the biocellulose are not detachable from each other, and thus a single sheet can be formed as a whole. In addition, a high elasticity effect such as vertical stress or elongation viscosity is imparted by entanglement, or tensile strength is high, so that the sheet is resistant to damage and has easy handling characteristics.

Non-woven fabric is a fiber with a flat structure made by forming a sheet-shaped web that tangles various fibers such as natural fibers, chemical fibers, glass fibers, and metal fibers according to mutual characteristics, and combined them by mechanical or physical methods. It is a structure. As the raw fiber, one or more selected from the group consisting of natural fibers and synthetic fibers may be used. For example, it may be a nonwoven material containing at least one selected from viscose rayon fiber, polypropylene fiber, polyethylene fiber, polyethylene terephthalate fiber, polyester fiber, nylon fiber and cellulose fiber. There is no particular limitation on the material or manufacturing method of the nonwoven fabric herein. Non-woven fabric is applicable as long as it is commonly used, preferably chemical bonding, thermal bonding, thermal bonding, air-ray non-woven fabric (Air Ray), wet non-woven fabric (Wet Ray), needle punching non-woven fabric (Needle) Punching, spanless (water zet), spun bond, melt blown, stitch bond and electrospinning spinning Can be

The nonwoven fabric may be a spunlace type nonwoven fabric that is commonly used, but is not limited thereto. The spunlace method is a method of joining the web by spraying high-pressure water flow after cutting the short fibers and stacking the web. A variety of designs can be obtained according to the pattern of the plate installed at the bottom of the web that receives the high-pressure water flow. This is possible and is excellent in flexibility and breathability and hygienic, so it is suitable as a cosmetic pack material or a medical patch material.

The biocellulose sheet provided herein may be a sheet in which biocellulose fibers are formed on one surface of a nonwoven fabric, such that fibers of the nonwoven fabric and biocellulose fibers are entangled, and biocellulose fibers are not formed on the opposite side of the nonwoven fabric. One surface of the non-woven fabric on which the bio-cellulose fibers are formed has a structure in which the fibers of the non-woven fabric and the bio-cellulose fibers are intertwined, and may be attached to the skin of the user's body. One surface of the non-woven fabric formed with bio-cellulose may include a plurality of protrusions to increase skin adhesion and penetrate the skin deeply through the capillaries of the skin, if necessary, but has special limitations in its shape. It is not.

The fibers of the nonwoven fabric can be microfibers. Non-woven fabrics are tens of micro-diameter-sized fibers and bio-celluloses of several nano-diameters can be formed thereon.

In the present invention, the weight ratio of the fibers of the nonwoven fabric and the biocellulose fiber may have various weight ratios depending on factors such as the type, shape, thickness, or density of the nonwoven fabric used even if the biocellulose weight is the same. Therefore, a person skilled in the art considers various factors such as tearing, handling, adhesion, fit, absorbency, or productivity (e.g., manufacturing time, cost, manufacturing amount), while biocellulose is sufficiently formed on the nonwoven fabric, and the fibers and bios of the nonwoven fabric are considered. It is apparent that a biocellulose sheet can be produced by appropriately adjusting the weight ratio of cellulose fibers.

In another embodiment, the present invention has a tangle strength greater than 0 N / m, specifically 0.1 N / m or higher, 0.2 N / m or higher, 0.3 N / m or higher, 0.4 N / m or higher, 0.5 N / m or higher, Provided is a biocellulose sheet having at least 0.6 N / m, at least 0.7 N / m, at least 0.8 N / m, at least 0.9 N / m, or at least 1.0 N / m. In addition, even if the entanglement strength is measured high, it is not a problem in terms of physical properties, but a person skilled in the art is capable of tearing, handling, adhesion, fit, absorbency, or productivity (e.g., manufacturing time, cost) while biocellulose is sufficiently formed on the nonwoven fabric. , Manufacturing amount), and the like, the entanglement strength can be adjusted within a desired range, for example, 100 N / m or less, 90 N / m or less, 80 N / m or less, 70 N / m or less, 60 N / m or less, 50 N / m or less, 40 N / m or less, 30 N / m or less, 20 N / m or less, or 10 N / m or less, but the present invention is not limited thereto.

According to one embodiment of the present application, the fiber entanglement strength in the nonwoven film detachably attached to the biocellulose sheet is almost 0, whereas the entanglement strength of the biocellulose contained in the sheet and the fiber of the nonwoven fabric is higher than that. By showing the numerical value, it was confirmed that a sheet having a structure in which fibers of the nonwoven fabric and biocellulose fibers were entangled was produced. Furthermore, by appropriately changing specific culture conditions (eg, culture period, inoculation bacteria concentration, amount of culture medium, etc.) in the production of biocellulose sheet, while maintaining the structure in which the fibers of the nonwoven fabric and the biocellulose fibers are entangled, as necessary It was confirmed that the mechanical strength of the biocellulose sheet can be appropriately adjusted or the biocellulose production amount can be easily adjusted.

In another embodiment, the present invention, the present invention has a tensile strength of more than 0 Mpa, specifically 0.1 Mpa or more, 0.2 Mpa or more, 0.3 Mpa or more, 0.4 Mpa or more, 0.5 Mpa or more, 0.6 Mpa or more, 0.7 Mpa or more, It provides a biocellulose sheet of 0.8 Mpa or more, 0.9 Mpa or more, or 1.0 Mpa or more. In addition, although the tensile strength is measured high, it does not pose a significant problem in terms of physical properties, but those skilled in the art are capable of tearing, handling, adhesion, fit, absorbency, or productivity (e.g., manufacturing time, cost, while biocellulose is sufficiently formed on the nonwoven fabric) , Manufacturing amount), the tensile strength can be adjusted within a desired range, for example, 100 Mpa or less, 90 Mpa or less, 80 Mpa or less, 70 Mpa or less, 60 Mpa or less, 50 Mpa or less, 40 It may be adjusted to Mpa or less, 30 Mpa or less, 20 Mpa or less, or 10 Mpa or less, but the present invention is not limited thereto.

According to one embodiment of the present application, the fiber tensile strength of the nonwoven fabric film detachably attached to the biocellulose sheet is almost zero, whereas the tensile strength of the sheet of the present application is higher than that of the nonwoven fabric. It was confirmed that a sheet having a structure in which biocellulose fibers were entangled was produced. Furthermore, by appropriately changing specific culture conditions (eg, culture period, inoculation bacteria concentration, amount of culture medium, etc.) in the production of biocellulose sheet, while maintaining the structure in which the fibers of the nonwoven fabric and the biocellulose fibers are entangled, as necessary It was confirmed that the mechanical strength of the biocellulose sheet can be appropriately adjusted or the biocellulose production amount can be easily adjusted.

Impregnating the non-woven fabric in the bio-cellulose culture according to another aspect of the present invention; And culturing the non-woven fabric impregnated with the culture solution.

The biocellulose culture medium contains nutrients necessary for the growth and development of microorganisms that produce biocellulose. It is possible to use a conventional culture medium used in the art to produce biocellulose. The culture medium may contain various carbon sources, nitrogen sources and / or trace element components. For example, a culture solution containing alcohol, protein, and yeast can be used.

In specific embodiments, the carbon source includes carbohydrates such as glucose, lactose, sucrose, fructose, maltose, starch and cellulose; Fats such as soybean oil, sunflower oil, castor oil and coconut oil; Fatty acids such as palmitic acid, stearic acid and linoleic acid; Alcohols such as glycerol and ethanol, and organic acids such as acetic acid. These carbon sources can be used alone or in combination.

Sources of nitrogen include organic nitrogen sources and urea (such as peptone, yeast extract, gravy, malt extract, corn steep liquor (CSL) and soy flour). urea), ammonium sulfate, ammonium chloride, ammonium phosphate, ammonium carbonate, and inorganic nitrogen sources such as ammonium nitrate. These nitrogen sources may be used alone or in combination.

If necessary, the culture medium may additionally include potassium dihydrogen phosphate, potassium dihydrogen phosphate, and corresponding sodium-containing salts as a phosphate source. In addition, it may include a metal such as magnesium sulfate (magnesium sulfate) or iron sulfate (iron sulfate). In addition, amino acids, vitamins and suitable precursors can be added.

In addition, the culture medium may contain plant extracts containing glucose to increase the production efficiency of biocellulose, for example, plant materials such as coconut, sugarcane, bokbunja, citrus fruits, pear, apple, grape, watermelon, corn It may include. In addition, any culture medium capable of producing biocellulose by fermenting the strain can be used without particular limitation.

The biocellulose sheet may be prepared by impregnating a non-woven fabric into a bio-cellulose culture solution inoculated with a strain, and then removing or culturing in an impregnated state to produce bio-cellulose on the non-woven fabric. As a strain to be inoculated into the culture medium, as a biosynthetic strain, Acetobacter genus, Gluconacetobacter genus, Agrobacterium genus, Lactobacillus genus, Rhizobium genus, Pseudomonas (Pseudomonas), Sarsina (Sarcina), and other microorganism strains may be used, but are not limited thereto. For example, Acetobacter xylinum, Acetobacter xylinus, Acetobacter pasteurinanus, Acetobacter pasteurinanus, A. hansenii, or gluconacetobacter xylinum (Gluconacetobacter xylinus) and the like can be used. A single strain can be cultivated, or multiple strains can be cultivated for higher production efficiency. For example, acetobacter xylium having excellent production yield and lactic acid bacteria producing bacteriocin to prevent contaminating bacteria may be statically mixed or stirred mixed.

When the strain is cultured, the pH range of 3 to 7 is appropriate, but is not limited thereto, and may be appropriately adjusted according to the inoculated bacteria. In addition, the culture temperature can be cultured at a temperature in the range of 20 ° C to 40 ° C, more specifically 20 to 30 ° C, and the culture time can be cultured for 1 day to 10 days, but the specific culture temperature or time is appropriately adjusted. It is possible to maintain optimum growth conditions of microorganisms producing biocellulose. Through such cultivation, microorganisms decompose organic substances using their own enzymes to produce biocellulose, which is called fermentation.

In addition, those skilled in the art will understand that the amount of medium added and the culture time may vary depending on the thickness of the biocellulose to be prepared. If necessary, additional medium may be added to prevent the nonwoven fabric from drying out during the culture period.

As the strain culture method, any culture method commonly used in the field to which the present invention pertains, such as a static cultivation method or an agitated cultivation method, may be used, but a static culture method may be used.

The static culture method is a method in which bacteria are first transplanted into a medium, and then cultured while being placed on a shelf for several hours to several days in a flask. For example, acetobacter xylium is an aerobic fungus that produces a thin, elastic film on the surface of the medium. The formed cellulose biofilm can be obtained by removing excess bacteria with an aqueous sodium hydroxide solution and at the same time obtaining pure cellulose. Thereafter, biocellulose may be obtained by repeatedly washing with distilled water.

Another method, the shaking culture method, is a method of culturing while continuously stirring at a constant rate in a shaking incubator through a liquid medium. The biocellulose thus formed becomes smaller in size than the crystallinity of the biocellulose cultured.

The biocellulose sheet is obtained by immediately dipping a nonwoven fabric cut into a shape suitable for the body part to be attached to immediately obtain a sheet having a desired shape, or by first preparing a sheet and then cutting it into a shape suitable for the body to be attached. Can get

In order for the biocellulose to be used as a cosmetic pack sheet, the microbial cells of the injected microorganisms for fermentation must be killed and fermented and the culture medium is removed, but the present invention is not limited thereto, and the microbial cells are killed and the culture medium is removed by repeating the hot water sterilization process. can do.

The prepared biocellulose sheet may be provided by slicing to an appropriate thickness. Although this thickness range is not particularly limited, when the thickness of the biocellulose becomes thinner, the skin adhesion is excellent, but the amount of fluid is reduced and dries quickly on the skin, and when the thickness is thick, the moisturizing power can be maintained for a long time on the skin. Falls. Therefore, the sheet can be usually provided in a thickness of 0.1mm to 5.0mm, a thickness of 0.5mm to 3.5mm, or a thickness of 0.05mm to 1mm, which is used as a cosmetic sheet by injecting a cosmetic or by injecting a pharmaceutical ingredient. In the case, it is a range suitable for including high water content while being easily attached to the body.

Conventional bio-cellulose sheet, after preparing a bio-cellulose sheet from a bio-cellulose culture medium inoculated with microorganisms, non-woven film, reticular fibrous film, polymer thin film, waterproof film, polymer foam film on one or both sides of the produced biocellulose sheet, Alternatively, a support such as a paper film was laminated to maintain the appearance of the sheet, which had to be removed immediately before the user used the product.

On the other hand, the bio-cellulose sheet provided herein is impregnated with a non-woven fabric in a bio-cellulose culture medium, so that bio-cellulose is formed on the non-woven fabric, so that the fibers of the non-woven fabric and the fibers of the bio-cellulose are entangled with each other. Accordingly, the non-woven fabric and bio-cellulose are not detachable from each other and constitute a sheet integrally, and are different from the bio-cellulose sheet to which the non-woven fabric film conventionally attached is attached.

The prepared biocellulose sheet can be dehydrated or dried to make it a constant water retention state. When the makeup fluid or the like is deposited after complete drying, the water retention of the biocellulose is poor and the swelling of the sheet takes too long or the swelling is insufficient, so the moisture content is maintained at a weight of 1 to 50 times the dry weight of the biocellulose sheet by the dehydration process. After impregnation with the impregnating liquid, a cosmetic emulsion or the like may be added, and more specifically, moisture in a weight range of 10 to 20 times the dry weight of the sheet may be maintained.

The prepared biocellulose sheet may be used as a cosmetic pack by injecting a cosmetic having a special purpose such as desired whitening, anti-wrinkle, and nutrition, or may be used as a medical patch such as for treatment of burns or wounds. For example, a mask pack covering the entire face, or a cosmetic pack applied to a specific area such as under the eyes, eyes, nose, mouth, cheeks, neck, or chin, anti-inflammatory patch for bruises, long-term patch for arthritis patients, and itching for atopic patients It may be used as a cooling agent, a wound protectant that can be used for cosmetic surgery, and a botox patch, but is not limited thereto.

Accordingly, according to one aspect of the present invention, a cosmetic pack including a biocellulose sheet is provided. In addition, a cosmetic pack in which a cosmetic ingredient is supported on a sheet is provided. According to another aspect of the invention, a medical patch comprising a biocellulose sheet is provided.

Cosmetics may include various functional ingredients according to purposes such as whitening, anti-wrinkle, skin elasticity improvement, skin immunity enhancement, moisturizing, nutrition supply, blemish removal, skin trouble relief, skin anti-inflammatory, and antioxidant. For example, glycerin, probiotic extract, trehalose, glucan, chitosan, vitamins, ceramide, collagen, hyaluronic acid, nicotinamide, adenosine, retinol, allantoin, arbutin, retinol, antioxidants, aroma oil, epithelial cell growth factor, platelets Derived growth factors, insulin growth factors, tea tree, polyphenols, intocyanidins, isoflavones, lutein, carotene, carotenoids, natural extracts (aloe extract, lily extract, seaweed extract rose extract, persimmon extract, jasmine extract, insensibility extract) , Mushroom extract, bamboo extract, apricot seed extract, cucumber extract, green tea extract, ginseng extract, red ginseng extract, mung bean extract, wormwood extract, etc.), or a mixture of at least two. These potent ingredients can be impregnated into the sheet in the form of an emulsion, more specifically an oil-in-water emulsion.

Hereinafter, the present invention will be described in more detail by the following examples. However, these are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Example 1. Preparation of biocellulose sheet

As an example, as a biocellulose culture, a culture medium containing 25 g of mannitol, 5 g of peptone, and 3 g of yeast was used as the culture medium, and gluconacetobacter xylinus (provided by the Korea Microbiological Conservation Center) 5 ml was inoculated. Specifically, 1/10 of G. xylinus bacteria cultured in a 90 mm Petri dish solid medium was collected and released in 50 ml of a culture medium having the composition described above. The miracloth nonwoven fabric (Sigma-Aldrich) was immersed in a culture medium to which bacteria were added, and then pulled out and spread on an empty petri dish. The normal medium without the addition of bacteria was added to the extent that the nonwoven fabric was submerged. After closing and sealing the petri dish lid, it was incubated for more than 3 days in a 26 ° C incubator.

As a comparative example, a biocellulose sheet was prepared by statically culturing a biocellulose culture having the same composition as above.

As a result of observation with a scanning electron microscope (SEM), in the case of the biocellulose sheet of the embodiment, biocellulose was not formed on one side of the sheet and only nonwoven fibers were observed, and on the other side of the sheet, the nanofiber size entangled with the microfiber of the nonwoven fabric Biocellulose was observed (Fig. 1).

Example 2. Measurement of tangle strength of biocellulose sheet

The 180 ° Peel test was performed with UTM equipment (Universal testing machine LRX plus model of Lloyd Instruments), and the entanglement strength of the biocellulose sheet of Example and the biocellulose sheet of Comparative Example was measured. The measurement conditions and results are as follows:

Specimen size: 25 * 100mm

Load cell: 10N

Propagation speed: 25mm / min

As a result of measuring the entanglement strength with a Peel test through repeated experiments, the entanglement strength of the biocellulose sheet of the example was 27.30 ± 2.23 N / m , And the entanglement strength of the biocellulose sheet of the comparative example was 0.30 ± 0.13 N / m As, the sheet of the example showed a strong entanglement strength of about 90 times.

In fact, in the case of the comparative example, the force should be 0 because it is not tangled, but it is considered that some force is measured due to the tension of the lotion.

Next, prepared in the same manner as in Example 1, for the biocellulose sheet prepared by reducing the culture period to 2 days, the entanglement strength was measured by the Peel test under the same measurement conditions as described above. As a result, the entanglement strength was 18.45 ± 3.81 N / m, and the entanglement strength of the comparative example was about 60 times stronger than the entanglement strength of 0.30 ± 0.13 N / m.

In addition, prepared in the same manner as in Example 1, for the biocellulose sheet produced by increasing the culture period to 10 days, as a result of measuring the entanglement strength under the same measurement conditions as described above, the entanglement strength is 33.18 ± 5.42 N / m As shown, the entanglement strength was about 110 times stronger than the comparative example.

As described above, the sheet of the structure in which the fibers of the nonwoven fabric and the biocellulose fibers are entangled can be produced by the method of the present application, and further, even if the manufacturing method of the biocellulose sheet such as the culture period is appropriately changed, the biocellulose sheet still has excellent tangle strength. It can be confirmed that can be produced. Therefore, a person skilled in the art can maintain the structure in which the fibers of the nonwoven fabric and the biocellulose fibers are entangled by appropriately changing specific culture conditions and the like in the production of the biocellulose sheet, while considering the mechanical strength in consideration of the adhesion, fit, or absorbency of the biocellulose sheet. It can be appropriately controlled or the biocellulose production amount can be easily adjusted.

Example 3. Measurement of tensile strength of biocellulose sheet

In order to support the properties of the biocellulose sheet according to the present application, which are resistant to damage and easy to handle, tensile strength of the biocellulose sheet of Example and the biocellulose sheet of Comparative Example was measured. Tensile strength was measured under the following conditions using UTM equipment (model 5982 from INSTRON):

Specimen size: 25 * 100mm

Load cell: 5KN

Propagation speed: 15mm / min

As a result of measuring the tensile strength, the tensile strength of the biocellulose sheet of the Example is 2.75 ± 0.44 Mpa , And the tensile strength of the biocellulose sheet of the comparative example was 0.085 ± 0.005 Mpa, and the sheet of the example showed about 32 times stronger tensile strength.

Next, prepared in the same manner as in Example 1, for the bio-cellulose sheet prepared by reducing the culture period to 2 days, as a result of measuring the tensile strength under the same measurement conditions as described above, the tensile strength is 1.23 ± 0.28 N / As m, the tensile strength was about 14 times stronger than the comparative example.

In addition, it was prepared in the same manner as in Example 1, for the bio-cellulose sheet prepared by increasing the culture period to 10 days, as a result of measuring the tensile strength under the same measurement conditions as described above, the tensile strength is 7.48 ± 0.57 N / m It showed a tensile strength of about 88 times stronger than the comparative example.

As described above, the sheet of the structure in which the fibers of the nonwoven fabric and the biocellulose fibers are entangled can be manufactured by the method of the present application, and further, even if the manufacturing method of the biocellulose sheet such as the culture period is appropriately changed, the biocellulose sheet still having excellent tensile strength It can be confirmed that can be produced. Therefore, a person skilled in the art can maintain the structure in which the fibers of the nonwoven fabric and the biocellulose fibers are entangled by appropriately changing specific culture conditions and the like in the production of the biocellulose sheet, while considering the mechanical strength in consideration of the adhesion, fit, or absorbency of the biocellulose sheet. It can be appropriately controlled or the biocellulose production amount can be easily adjusted.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing its technical spirit or essential features. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the following claims rather than the detailed description and equivalent concepts thereof.

Claims (12)

1. Non-woven fabric and bio-cellulose,

   It has a structure in which the fibers of the nonwoven fabric and the biocellulose fibers are entangled,

   Biocellulose sheet.
2. The biocellulose sheet according to claim 1, wherein a structure in which fibers of the nonwoven fabric and biocellulose fibers are entangled is formed on one surface of the nonwoven fabric, and biocellulose fibers are not formed on the opposite side of the nonwoven fabric.
3. The biocellulose sheet according to claim 1 or 2, wherein the sheet has a tensile strength of 1.0 Mpa or more.
4. The biocellulose sheet according to any one of claims 1 to 3, wherein the sheet has an entanglement strength of 1.0 N / m or more.
5. The biocellulose sheet according to any one of claims 1 to 4, wherein the fibers of the nonwoven fabric are microfibers.
6. The biocellulose sheet according to any one of claims 1 to 5, wherein the nonwoven fabric comprises at least one selected from the group consisting of natural fibers and synthetic fibers.
7. Impregnating the bio-cellulose culture with a nonwoven fabric; And

   Comprising the step of culturing the non-woven fabric impregnated with the culture solution,

   The manufacturing method of the biocellulose sheet of any one of Claims 1-6.
8. The method according to claim 7, wherein the culture medium contains alcohol, protein, yeast, and microorganisms.
9. The production method according to claim 7, wherein the culture is stationary culture.
10. A cosmetic pack comprising the biocellulose sheet of claim 1.
11. The cosmetic pack according to claim 10, wherein the cosmetic ingredient is supported on a sheet.
12. A medical patch comprising the biocellulose sheet of claim 1.

Images