KR102084943B1 - The method for manufacturing biocellulose mask pack by using maturing propolis, and a mask pack manufactured by the method - Google Patents

Abstract

The present invention relates to a method for manufacturing wet and dry biocellulose mask packs containing aged propolis, which can be stored for a long time without using a chemical preservative, and has no skin irritation and improves skin adhesion by controlling the strength of biocellulose. According to the preparation method according to the present invention, after adding 5.0 parts by weight of jujube and 5.0 parts by weight of jujube leaves removed from seeds to 100 parts by weight of propolis solution, the mixture was heated and maintained at 70 ° C. for 15 hours, cooled, and then cooled to a low temperature of 5 ° C. Aging for 30 days to prepare a aging propolis; And when the thickness of the biocellulose produced while producing the biocellulose by using the culture solution is 3mm, add 100 parts by weight of 1.0 parts by weight of the biocellulose to the aged propolis and leave for 36 hours at 30 ℃, recovery of the biocellulose 5mm There is no skin irritation because there is no need to use a chemical preservative, including the step of adjusting the strength and thickness of the bio cellulose to improve the skin adhesion by adjusting the strength of the bio cellulose.

Description

Biocellulose mask pack containing aged propolis and manufacturing method thereof {THE METHOD FOR MANUFACTURING BIOCELLULOSE MASK PACK BY USING MATURING PROPOLIS, AND A MASK PACK MANUFACTURED BY THE METHOD}

The present invention relates to a wet and dry bio cellulose mask pack and a method for manufacturing the same, containing aged propolis, which can be stored for a long period of time without using a chemical preservative, which has no skin irritation and improves skin adhesion by adjusting the strength of biocellulose.

Mask pack is a product that is configured to be in contact with the skin for a long time by impregnating a liquid or gel cosmetic composition on the support sheet for skin health, such as moisturizing the skin, removing old waste, whitening, and improving skin health. it means.

Mask packs currently used include, but are not limited to, peel-off type, wash-off type, tissue-off type, sheet type, and powder type. Because of the convenience of the sheet type (Sheet) is the most commonly used.

In general, in the cosmetic field, a sheet-type pack is a product that can be moisturized and clean by attaching a sheet made in a specific form to a human body without applying by hand. Sheet-type packs have a support that can hold the fluid so that it can act on the skin for a long time as compared to a general cosmetic emulsion. The support is in the form of a cosmetic product having excellent moisturizing effect and skin cosmetic effect due to enhanced skin penetration of the active ingredient due to the occlusive effect.

Such cosmetic sheets generally use a nonwoven fabric made of plant biocellulose (Cellulose) fibers or synthetic fibers from cotton or pulp as a freshener and mask-like support of the cosmetic emulsion. At this time, it can be given a variety of feeling and efficacy depending on the material, type, raw materials of the nonwoven fabric to be applied, and in addition to the fiber nonwoven fabric, a variety of unique materials can be used as a freshener of the cosmetic emulsion.

On the other hand, biocellulose, a biocellulose produced by the Acetobacter xylinus strain, was first reported in 1886 by Brown (Brown A, 1986). It is recognized.

Biocellulose is composed of pure biocellulose only compared to plant biocellulose, has a high physical structure with ultra fine network structure, and biodegradability that is naturally decomposed compared to general biocellulose. Because of its high moisture retention, excellent physical strength and transparency, it can be used as a bio-friendly material.

Therefore, a variety of industrial research and development and application, such as food, cosmetics, medical care using biocellulose has attracted attention.

Biocellulose can be produced by microorganisms, and the resulting biocellulose is a ribbon-shaped fibrous cellulose, which is smaller than 100 nm in width and has a radius of 2 to 4 nm. It consists of fibers and has a density of 1600 kg / m ³ .

Biocellulose shows high crystallinity (84 ~ 89%) depending on physical structure, which is higher than general fine natural fiber, and elastic modulus is similar to glass fiber.

In addition, biocellulose produced by microorganisms is highly biocompatible and is used for medical materials such as artificial cartilage, artificial blood vessels, dressings, and burn treatments. Typically, Dermafill (Georgia, USA) products are widely used. . In addition, compared to the hydrogel mask pack developed in place of the nonwoven fabric sheet as a cosmetic material, it has less irritation and is composed of nano size, which has a wider surface area and higher tensile strength than the general nonwoven mask pack, and has excellent skin adhesion and 10 times wettability. It can increase skin absorption rate.

Biocellulose is produced by algae, fungi, acetobactersp, agrobacterium sp, pseudomonas sp, and rhizobium sp. It has been reported to be produced by strains of the genus Alcaligenes sp, Gluconobacter sp and the like.

As a related art, a method of producing Gluconacetobacter persimmonensis KJ145 (KCTC 10175BP) and a bacterium-derived biocellulose using the same, which is excellent in producing bacterial biocellulose, is also known. A novel strain of gluconacetobacter, which can produce cellulose in very high yields, has also been disclosed. It is resistant to ethanol and stably maintained in shear force even in agitated cultures, as well as several acetobacter xylium strains (BRC5). , BPR2001, etc.), a novel acetobacter xylinum KJ-1 strain (Acetobacter xylinum KJ-1) and a method for producing a bacterial biocellulose using the same are also known.

However, a mask pack made of biocellulose has a good environment for microorganisms to grow, and thus, a preservative is essential. Conventionally, preservatives of parabens have been used to suppress microorganisms or molds. However, since the allergens such as allergy of parabens preservatives are emerging, use of a preservative that can replace parabens preservatives is required.

In addition, biocellulose has different strengths and thicknesses depending on the culture conditions and the culture time. In order to use biocellulose as a mask pack, it is preferable to form it with appropriate intensity and thickness. Thick and hardly formed biocellulose is difficult to properly hydrate and nourish the skin because it is difficult to adhere to the curved skin when manufactured as a mask pack. Accordingly, there is a demand for manufacturing the thickness and strength of the biocellulose sheet to suit the mask pack.

1. Republic of Korea Patent Publication No. 10-0454911 2. Republic of Korea Patent Publication No. 10-2011-0100139 3. Republic of Korea Patent Publication No. 10-2003-0088596

The technical problem of the present invention is to provide a mask pack capable of controlling the strength and thickness of the biocellulose in the manufacturing process of the biocellulose, which does not use a chemical preservative with high skin irritation, and provides an antioxidant action and nutrition, and a method of manufacturing the same. will be.

In order to achieve the above technical problem, the present invention,

After adding 5.0 parts by weight of jujube and 5.0 parts by weight of jujube with seeds removed to 100 parts by weight of propolis solution, the mixture was heated at 70 ° C. for 15 hours, concentrated, cooled, and aged at 30 ° C. for 30 days. Preparing a aged propolis; And

When the biocellulose produced using the culture solution is 3mm in thickness, 100 mg by weight of biocellulose is added to 1.0 part by weight of biocellulose, and left at 30 ° C. for 36 hours. Controlling the strength and thickness of the biocellulose

It provides a method for producing a biocellulose mask pack containing the aged propolis, characterized in that it comprises a.

The method for producing a biocellulose mask pack containing the aged propolis according to the present invention as described above may further include drying the recovered biocellulose to produce a dried biocellulose.

In the method for producing a biocellulose mask pack containing the aged propolis according to the present invention as described above, the step of producing biocellulose using a culture solution is

Culturing Acetobacter xylinum to obtain acetobacter xylinum broth;

Culturing Peacha fermentans to obtain a Peacha permantans culture solution;

Spawn medium After the culture of the acetobacter xylinum solution is added, the acidity is lowered to prepare a spawn solution for mass culture by adding a culture solution of Peacha permantans;

It may include the step of culturing after the addition of the seed culture solution for mass culture in a medium for mass culture to produce biocellulose.

In the method for producing a biocellulose mask pack containing the aged propolis according to the present invention as described above, the aged propolis penetrates into the biocellulose by immersing in a liquid to which 5 parts by weight of the aged propolis is added to 95 parts by weight of purified water for 18 hours. It may further comprise a step to make.

In addition, the present invention to achieve another technical problem,

After adding 5.0 parts by weight of jujube and 5.0 parts by weight of jujube with seeds removed to 100 parts by weight of propolis solution, the mixture was heated at 70 ° C. for 15 hours, concentrated, cooled, and aged at 30 ° C. for 30 days. Preparing a aged propolis; And

When the biocellulose produced using the culture solution is 3mm in thickness, 100 mg by weight of biocellulose is added to 1.0 part by weight of biocellulose, and left at 30 ° C. for 36 hours. Controlling the strength and thickness of the biocellulose

It provides a biocellulose mask pack containing the aged propolis prepared by a manufacturing method comprising.

The mask pack according to the present invention and a method for manufacturing the same have the effect of providing a mask pack free of skin irritation since there is no need to use a chemical preservative using aged propolis.

In addition, it has the effect of improving the skin adhesion by adjusting the strength of the biocellulose.

Hereinafter, the present invention will be described in detail with reference to the following examples.

Biocellulose manufacturing step of the following embodiment is one example for producing a biocellulose limited to this is sufficiently replaceable that the biocellulose manufacturing method well known in the art.

In addition, the present invention is divided into a wet bio cellulose and a dry bio cellulose according to the production method of the bio cellulose can be applied without being divided. That is, when the biocellulose produced in the manufacturing method of the following example is dried, it becomes a dry biocellulose, and the drying method is a method of immersing the biocellulose in a dehydrating agent such as a glycol-based compound, and adhering the biocellulose to a dry nonwoven fabric to obtain moisture. As long as it is well known in the art, such as how to remove the present invention can be used without particular limitation. Such dry biocellulose is ideal as a mask pack sheet material having a moisture content of more than 200 times the dry weight.

The core of the present invention relates to a method for producing wet and dry biocellulose mask packs containing aged propolis, which can be stored for a long time without using chemical preservatives, thereby preventing skin irritation and improving skin adhesion by controlling the strength of biocellulose.

After adding 5.0 parts by weight of jujube and 5.0 parts by weight of jujube with seeds removed to 100 parts by weight of propolis solution, the mixture was heated at 70 ° C. for 15 hours, concentrated, cooled, and aged at 30 ° C. for 30 days. Preparing a aged propolis; And

When the biocellulose produced using the culture solution is 3mm in thickness, 100 mg by weight of biocellulose is added to 1.0 part by weight of biocellulose, and left at 30 ° C. for 36 hours. It characterized in that it comprises the step of adjusting the strength and thickness of the biocellulose

In other words, the present inventors have found that the propolis that is aged in the manufacturing process of the biocellulose shows a better effect than the use of the non-aged propolis, and thus, the present invention has been completed. After putting the yellow lacquer leaves and concentrating under heat, the concentrated solution was matured at low temperature, and then used as a aging propolis.

According to the present invention as shown in the present invention, when the non-mature general propolis shows an excellent effect in antibacterial activity, skin irritation, skin adhesion (feeling).

Hereinafter, the present invention will be described in detail with reference to Example 1.

Example  1.Containing Aged Propolis Biocellulose  Manufacture of mask packs

(1) acetobacter Xylinum  Step of obtaining culture

Acetobacter xylinum (Actobacter xylinum) is incubated for 30 hours in a 30 ℃ liquid medium containing 50g / l glucose, 10g / l yeast extract and 30g / l calcium carbonate, to obtain acetobacter xylinum culture.

(2) Peachia Permantans  Step of obtaining culture

Pichia fermentans were incubated for 30 hours in a liquid medium at 25 ° C. containing 50 g / l of glucose, 10 g / l of yeast extract, 5 g / l of peptone and 10 g / l of malt extract. Get

(3) for mass cultivation Spawn  Manufacturing steps

2 parts by weight of the acetobacter xyllium culture medium was added to 100 parts by weight of the seed culture medium containing 50 g / l of immature apples, 30 g / l of sugar and 0.5 g / l of molasses, and the plate was cultured at 30 ° C. for 23 hours. When it is lowered, 2 parts by weight of the culture solution of Peacha Permantans is added and incubated at 25 ° C for about 7 days. When biocellulose is about 5 mm in the upper part, the lower liquid is used as a spawn solution for mass culture.

(4) producing biocellulose

After adding 0.1 parts by weight of the seed culture solution for mass culture to 100 parts by weight of the culture medium containing 50 g / l of immature apples, 30 g / l of sugar and 0.5 g / l of molasses, the culture medium was incubated at 30 ° C. for 7 days. Cellulose can be produced.

(5) Preparation of Aged Propolis

After adding 5.0 parts by weight of jujube and 5.0 parts by weight of jujube with seeds removed to 100 parts by weight of propolis solution, the mixture was heated at 70 ° C. for 15 hours, concentrated, cooled, and aged at 30 ° C. for 30 days. Aged propolis was prepared.

(6) Biocellulose  Steps to Adjust Strength and Thickness

When the thickness of the biocellulose produced in the step of producing the biocellulose is 3mm, 100 parts by weight of 1.0 part by weight of the cultured propolis is added and left at 30 ° C. for 36 hours, and the biocellulose is recovered when 5 mm is obtained.

Biocellulose prepared in this manner has the advantage that the action of the two strains by the antimicrobial action of the aging propolis is prepared with a softer strength than when the aging propolis is not added.

If the strength of the biocellulose is hard, it is difficult to adhere completely to curved skin, resulting in poor adhesion and contact feeling. Inferior adhesion mask pack has a problem that is difficult to deliver effective moisturizing and nutrients. Through adjusting the strength and thickness of the bio cellulose, it is possible to manufacture a mask pack with improved adhesion and contact feeling.

(7) Biocellulose in aged propolis liquid Immersed  step

Immediately after incubation, the biocellulose has a strong acetic acid odor, so it is washed three or more times with purified water to remove the odor.

Next, after dehydration so that the ripened propolis solution can be absorbed well, it is immersed in 95 parts by weight of purified water for 5 hours by adding 5 parts by weight of mature propolis so that the solution containing the ripened propolis infiltrates the biocellulose. do.

Biocellulose has a good environment for microorganisms to grow, so preservatives must be used. Conventionally, parabens preservatives have been used to suppress microorganisms and mold. However, paraben preservatives are causing harmful effects such as allergies, and use of a preservative that can replace paraben preservatives is required.

Accordingly, by using a aging propolis having the function of antibacterial action, anti-inflammatory action, antioxidant action, immune action and antiviral action, it prevents the growth of microorganisms and has a positive effect on the skin.

(8) forming and packing the mask pack

The biocellulose immersed in the preservative solution containing the aged propolis is taken out and compressed to remove 80 to 90% of water. Next, after cutting the biocellulose to the desired size, the mask pack is put in a wrapping paper and injected with a cosmetic (essence) and then packed.

In the pressing process, a mask pack of various types and sizes including biocellulose may be manufactured by attaching a base agent including a nonwoven fabric, a mesh, and a film to one or both surfaces of the biocellulose.

In addition, it is possible to prepare a biocellulose mask pack in which the effect of the aging propolis is further doubled by adding the aging propolis as a component of the cosmetic.

Experimental Example  1. Aging of Propolis  To use  Microbial growth prevention effect

[Preparation of experiment]

Experimental group 1 was prepared according to the preparation method described in Example 1, experimental group 2 was prepared according to the preparation method described in Example 1, but (7) instead of the step of immersing the biocellulose in the liquid containing the aging propolis instead It was prepared by adding 0.4 parts by weight of isobutylparaben (lsobutylparaben) instead of the aged propolis as a preservative.

In addition, Experimental Group 3 was prepared according to the preparation method described in Example 1, but (7) instead of immersing the biocellulose in the liquid containing the aging propolis without the addition of the aging propolis of the same weight unmatured concentrated propolis The experimental group 4 was prepared according to the preparation method described in Example 1, but was prepared by immersing in a liquid containing only purified water instead of immersing the biocellulose in a liquid containing the aged propolis (7). .

[Experimental]

At this time, the fungus Pytirosporum Ovalae (ATCC 12078) was used, and the antimicrobial activity was measured by the disk diffusion method.

Specifically, the test cells were incubated after placing the biocellulose sheet prepared with the solid medium inoculated with the test bacteria. After incubation, the relative antimicrobial activity was measured by measuring the size of the clear zone in which the growth of bacteria was suppressed. The experimental results are shown in Table 1 below as average values of the results of three measurements.

division
Inhibitor size (mm)
Experimental group 1
(ferment
Propolis)
Experiment group 2
(Isobutyl
Parabens)
Experiment group 3
(Unmatured
Propolis)
Experimental Group 4
(Purified water)
Suppression ring size
2.8
3.1
3.7
6.7

In Table 1, the experimental group 1 according to the present invention showed a better antimicrobial effect than the experimental group 2 using the chemical preservatives parabens, the experimental group 3 using the propolis not subjected to the aging process according to the present invention antibacterial effect Although the effect was less than the experimental group 1, the experimental group 4 used only purified water showed little antibacterial effect.

In other words, when using other aged propolis-containing liquid in the present invention it can be seen that the use of conventional parabens can suppress the growth of microorganisms better, even when manufacturing a mask pack having a long shelf life According to the invention there is no need to use chemical preservatives

Experimental Example  2: skin irritation evaluation test

In order to evaluate skin irritation using the sheets of Experimental Groups 1 to 3, a primary stimulation test was performed on a human body through a human patch test in the following manner.

CTFA Guidelines for 30 Healthy Adult Women [Reference: The Cosmetic, Toility and Fragrance Association. Inc. Washington, D.C., 20036.19941].

Fix the mask pack sheet on the back of the human body, which is the test site. It was then patched for 24 hours and then removed and the skin reaction at the test site was determined according to the following criteria and the results are shown in Table 2 below.

<Judgment criteria>

-: No erythema, no special phenomenon

+/-: slightly reddish

+: Significantly redder than the surroundings

++: reddens and swells more heavily than the surroundings

Stimulus = [{(+/-) number x 1} + {(+) number x 2} + {(++) number x 3}] / number of subjects

sample Number of test subjects Judgment result Irritation ++ + +/- - Experimental group 1 30 0 0 One 29 0.03 Experiment group 2 30 0 One 4 25 0.2 Experiment group 3 30 0 0 3 27 0.1

As shown in Table 2, the experimental group 1 using the aging propolis significantly reduced the skin irritation than the experimental group 2 using the chemical preservative parabens, and showed that the skin irritation was significantly reduced than the experimental group 3 using the non-aging propolis. Could.

Therefore, according to the present invention, not only the skin irritation-reducing effect of not using a chemical preservative but also the minute skin irritation that may be exhibited by propolis by aging can be significantly reduced.

Experimental Example  3. Feeling  And satisfaction assessment

[Preparation of experiment]

Experimental group 1 was prepared according to the preparation method described in Example 1, experimental group 2 was prepared according to the preparation method described in Example 1, but (6) non-aging instead of aged propolis in the step of adjusting the strength and thickness of the biocellulose Flopolis was used, and in the case of Experiment 3, (6) there was a difference in not using propolis itself in adjusting the strength and thickness of biocellulose.

[Experimental]

Mask packs of Experimental Groups 1 to 3 were provided, and each pack was attached and used three times for 20 minutes once every two days, followed by evaluation. The results are shown in Table 3.

30 women in their 20s and 40s participated in the sensory evaluation, and the 7-point scale was used for the evaluation. (1 point, very good; 2 points, little good; 3 points, bad; 4 points, normal; 5 points, a little good; 6 points, good; 7 points, very good).

[Experiment result]

Test Items Tightness Soft Moisturizing Overall satisfaction Experimental group 1 6.51 ± 0.15 6.51 ± 0.12 6.52 ± 0.15 6.51 ± 0.15 Experiment group 2 6.07 ± 0.57 6.07 ± 0.15 6.06 ± 0.12 6.07 ± 0.68 Experiment group 3 4.02 ± 0.56 4.03 ± 0.40 4.02 ± 1.01 4.02 ± 1.03

Looking at Table 3, it can be seen that the experimental group 1 received a good evaluation in all items, including overall satisfaction. Satisfaction group 2 was also higher than the experimental group 3, but less than the experimental group 1, it can be seen that the experimental group 3 received a poor evaluation compared to the experimental groups 1 to 2 in all items, including the overall satisfaction.

In other words, it is known that the biocellulose mask pack is superior in the use and moisturizing feeling than the mask pack using the nonwoven fabric. It was found to be superior to biocellulose and showed even better feeling of use and moisturizing especially when using aged propolis according to the present invention.

Therefore, the mask pack according to the present invention and a method for manufacturing the same have the effect of providing a mask pack free of skin irritation by using a aged propolis, without using a chemical preservative. In addition, it has the effect of improving the skin adhesion by adjusting the strength of the biocellulose.

Claims (5)

After adding 5.0 parts by weight of jujube and 5.0 parts by weight of jujube with seeds removed to 100 parts of propolis solution, the mixture was heated for 15 hours at 70 ° C, concentrated, cooled, and aged for 30 days at a low temperature of 5 ° C. Preparing a aged propolis;
When the biocellulose produced using the culture solution is 3mm in thickness, 100 mg by weight of biocellulose is added to 1.0 part by weight of biocellulose, and left at 30 ° C. for 36 hours. Adjusting the strength and thickness of the biocellulose; And
Dehydrating the recovered biocellulose and immersing it in a solution in which 5 parts by weight of the aged propolis was added to 95 parts by weight of purified water for 18 hours to allow the matured propolis to penetrate the biocellulose.
Biocellulose mask pack manufacturing method containing the aged propolis, characterized in that it comprises a.
The method of claim 1,
The step of producing biocellulose using the culture
Culturing Acetobacter xylinum to obtain acetobacter xylinum broth;
Culturing Peacha fermentans to obtain a Peacha permantans culture solution;
Spawn medium After the culture of the acetobacter xylinum solution is added, the acidity is lowered to prepare a spawn solution for mass culture by adding a culture solution of Peacha permantans;
The method for producing a biocellulose mask pack containing aging propolis, comprising the step of culturing the biomass after adding the seed culture solution for mass culture to a mass culture medium.
After adding 5.0 parts by weight of jujube and 5.0 parts by weight of jujube with seeds removed to 100 parts of propolis solution, the mixture was heated for 15 hours at 70 ° C, concentrated, cooled, and aged for 30 days at a low temperature of 5 ° C. Preparing a aged propolis;
When the biocellulose produced using the culture solution is 3mm in thickness, 100 mg by weight of biocellulose is added to 1.0 part by weight of biocellulose, and left at 30 ° C. for 36 hours. Adjusting the strength and thickness of the biocellulose; And
Dehydrating the recovered biocellulose and immersing it in a solution in which 5 parts by weight of the aged propolis was added to 95 parts by weight of purified water for 18 hours to allow the matured propolis to penetrate the biocellulose.
Biocellulose mask pack containing aged propolis prepared by a manufacturing method comprising delete delete