WO2020067648A3 - 수지상세포를 이용한 면역 소재의 면역증진 효과 평가방법 및 면역 소재 스크리닝 방법 - Google Patents

수지상세포를 이용한 면역 소재의 면역증진 효과 평가방법 및 면역 소재 스크리닝 방법 Download PDF

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WO2020067648A3
WO2020067648A3 PCT/KR2019/010689 KR2019010689W WO2020067648A3 WO 2020067648 A3 WO2020067648 A3 WO 2020067648A3 KR 2019010689 W KR2019010689 W KR 2019010689W WO 2020067648 A3 WO2020067648 A3 WO 2020067648A3
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immunogenic material
dendritic cells
immunity enhancement
immunogenic
screening
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PCT/KR2019/010689
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French (fr)
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WO2020067648A2 (ko
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권재열
김예은
선푸름
강성욱
이영하
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충남대학교 산학협력단
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Publication of WO2020067648A3 publication Critical patent/WO2020067648A3/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70546—Integrin superfamily, e.g. VLAs, leuCAM, GPIIb/GPIIIa, LPAM
    • G01N2333/70553—Integrin beta2-subunit-containing molecules, e.g. CD11, CD18
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30 CD40 or CD95

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
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  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
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  • General Physics & Mathematics (AREA)
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  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 수지상세포를 이용하여 면역소재의 면역증진 또는 면역개선 효과 평가방법 및 면역기능 소재의 스크리닝 방법에 관한 것으로서, 보다 상세하게는 (A) 수지상세포에 대상 소재 추출물을 처리한 처리구와, 블랭크를 처리한 대조구를 배양하는 단계; (B) 유세포분석법으로, 상기 처리구와 대조구에서, MHC II가 발현되지만 CD40은 발현되지 않는 그룹(A0)과, MHC II가 강하게 발현되면서 CD40도 발현되는 그룹(A1)의 세포비율을 측정하는 단계; 및 (C) 하기 식에 따라 상기 대상 소재의 면역증진 효과를 수치화하는 단계;를 포함하는 면역 소재의 면역증진 효과 평가방법에 관한 것이다.
PCT/KR2019/010689 2018-09-27 2019-08-22 수지상세포를 이용한 면역 소재의 면역증진 효과 평가방법 및 면역 소재 스크리닝 방법 WO2020067648A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2018-0114899 2018-09-27
KR1020180114899A KR101981147B1 (ko) 2018-09-27 2018-09-27 수지상세포를 이용한 면역 소재의 면역증진 효과 평가방법 및 면역 소재 스크리닝 방법

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WO2020067648A2 WO2020067648A2 (ko) 2020-04-02
WO2020067648A3 true WO2020067648A3 (ko) 2020-05-22

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WO (1) WO2020067648A2 (ko)

Families Citing this family (2)

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Publication number Priority date Publication date Assignee Title
KR101981147B1 (ko) * 2018-09-27 2019-05-22 충남대학교산학협력단 수지상세포를 이용한 면역 소재의 면역증진 효과 평가방법 및 면역 소재 스크리닝 방법
KR20220109291A (ko) 2021-01-28 2022-08-04 충남대학교산학협력단 면역기능 예측을 위한 바이오마커 및 이를 활용한 면역기능 예측방법

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KR101981147B1 (ko) 2019-05-22

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