WO2018067068A8 - Method of engineering microorganisms for the overproduction of short branched-chain fatty acids - Google Patents

Method of engineering microorganisms for the overproduction of short branched-chain fatty acids Download PDF

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Publication number
WO2018067068A8
WO2018067068A8 PCT/SG2017/050490 SG2017050490W WO2018067068A8 WO 2018067068 A8 WO2018067068 A8 WO 2018067068A8 SG 2017050490 W SG2017050490 W SG 2017050490W WO 2018067068 A8 WO2018067068 A8 WO 2018067068A8
Authority
WO
WIPO (PCT)
Prior art keywords
fatty acids
chain fatty
overproduction
short branched
methods
Prior art date
Application number
PCT/SG2017/050490
Other languages
French (fr)
Other versions
WO2018067068A1 (en
Inventor
Matthew Wook Chang
Ai-qun YU
Nina Kurniasih PRATOMO JUWONO
Original Assignee
National University Of Singapore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Of Singapore filed Critical National University Of Singapore
Publication of WO2018067068A1 publication Critical patent/WO2018067068A1/en
Publication of WO2018067068A8 publication Critical patent/WO2018067068A8/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids

Abstract

The present invention relates to methods of metabolic engineering to increase the levels of short branched-chain fatty acids (SBCFAs) produced by microorganisms such as yeast. More particularly, the invention provides methods to engineer Saccharomyces cerevisiae to overexpress Ehrlich pathway genes, inhibit at least one alcohol dehydrogenase and enhance secretion of the produced SBCFAs, and also provides recombinant cells produced by such methods.
PCT/SG2017/050490 2016-10-07 2017-09-29 Method of engineering microorganisms for the overproduction of short branched-chain fatty acids WO2018067068A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662405502P 2016-10-07 2016-10-07
US62/405,502 2016-10-07

Publications (2)

Publication Number Publication Date
WO2018067068A1 WO2018067068A1 (en) 2018-04-12
WO2018067068A8 true WO2018067068A8 (en) 2018-05-24

Family

ID=61831844

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SG2017/050490 WO2018067068A1 (en) 2016-10-07 2017-09-29 Method of engineering microorganisms for the overproduction of short branched-chain fatty acids

Country Status (1)

Country Link
WO (1) WO2018067068A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109234303A (en) * 2018-10-12 2019-01-18 天津科技大学 The construction method of phenylpyruvate decarboxylase Aro10 is expressed in a kind of brewer's yeast
FR3122436A1 (en) 2021-04-30 2022-11-04 IFP Energies Nouvelles Multicopy insertion of a gene of interest into the genome of a fungus

Also Published As

Publication number Publication date
WO2018067068A1 (en) 2018-04-12

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