WO2020044499A1

WO2020044499A1 - Euglena aquaculture plant

Info

Publication number: WO2020044499A1
Application number: PCT/JP2018/032144
Authority: WO
Inventors: 嗣光松井
Original assignee: 嗣光松井
Priority date: 2018-08-30
Filing date: 2018-08-30
Publication date: 2020-03-05
Also published as: JPWO2020044499A1; JP7178008B2

Abstract

[Problem] To apply a magnetic field that exerts a good effect on the biological functions of Euglena and to promote Euglena photosynthesis by applying oxygen and carbon dioxide gas as well as to increase the dissolved oxygen to inhibit the generation of anaerobic microorganisms and thereby improve the Euglena growth environment. [Solution] A magnetizing device 10 composed of a rotating body 1 equipped with an electromagnetic inducer comprising a plurality of permanent magnets 1a and a motor 2 which rotates the rotating body 1 is mounted to create an alternating magnetic field in a water supply channel 11a to an aquaculture water tank 19 in which Euglena are present, magnetic bands 8 electrically connected to the magnetizing device 10 are placed in the aquaculture water tank 19 and the water supply water channel 11a and the aquaculture water tank 19 or the water supply channel is equipped with an aeration means, and an oxygen gas mixing means 20 for supplying oxygen and a carbon dioxide gas mixing means 21 for supplying carbon dioxide gas are provided in the aquaculture water tank 19.

Description

Euglena aquaculture plant

The present invention relates to a culture plant for Euglena, and more particularly, to a culture plant capable of efficiently increasing the production of Euglena by magnetizing and aerating storage and supply water in which Euglena exists.

Euglena is a plant classified in the Euglena plant phylum, is also an animal belonging to the Protozoan phylum, and subfamily Pteratophyta, and is a protist belonging to both animals and plants. Euglena is a unicellular eukaryote, and is classified into both plant and animal divisions in the taxonomics. In the plant division, euglena is in the euglena plant division, and in the animal division, the flagellate in the protozoan division. Belongs to. Euglena is composed of excellent animal proteins, has an amino acid balance close to milk protein casein, and contains moderate amounts of vitamins and inorganic salts, so it is reported that it is more nutritious than the alga Chlorella and Spirulina. I have.

Euglena grows under various growth conditions, and many kinds of useful substances contained in it contain highly unsaturated fatty acids that are not synthesized in the human body. . For example, using a culture flask, a culture tank, or the like, the cells are cultured by a batch culture method. Generally, only the temperature and the initial pH are controlled, and the cells are grown without controlling dissolved oxygen, light irradiation intensity, stirring, and the like. .
For this reason, productivity and quality are inadequate, and they have not been effectively used while having excellent food value, pharmaceutical materials, and potential value as fuel production materials.

For example, Patent Literature 1 discloses that during the cultivation of Euglena, aeration of high-concentration oxygen suppresses the production and secretion of waxy substances and mucous substances, and the synthesis of nutrients such as unsaturated fatty acids and vitamin E A method of cultivating Euglena for the purpose of promoting is described. In addition, Patent Document 2 discloses a method in which the carbon concentration and the nitrogen concentration are adjusted and supplied, and the Euglena cells are continuously cultured. It has been shown that 5 to 10-fold Euglena cells can be obtained, and that the Euglena cells have an intracellular protein content comparable to that of conventional Euglena cells.

Furthermore, Patent Document 3 (Japanese Patent Application Laid-Open No. 61-40785) discloses a method for culturing Euglena cells, which combines culturing under closed system darkness and culturing under open system light irradiation. Patent Document 4 discloses that a gas component necessary for culture is spouted and supplied toward the periphery of the bottom of the culture tank below the culture tank, and the culture solution is rotated radially from the rotation axis so as to rotate along the inner wall of the culture tank. The stirring is performed by a stirring rotary blade composed of a protruding radiation blade and an inclined blade having an area larger than that of the radiation blade and having an angle of 35 to 55 ° with respect to the radiation blade. A method and apparatus for culturing Euglena are shown.
And according to the said apparatus, the up and down stirring that the flow of a growth liquid rises so that the flow of a growth liquid turns on the inner wall surface of a culture tank by the stirring impeller, and falls along the circumference of a rotation axis is naturally comprised. . In addition, since the algal bodies that tend to concentrate around the bottom of the culture tank are dispersed by the jetting force of the gas, the algal bodies can be dispersed without applying mechanical stress. Then, the algal bodies dispersed by the gas jet power are swirled by the rotating blades, rise along the inner wall of the tank, and descend near the axis. It has been shown.

Patent Document 5 discloses that a medium having a pH of 1.8 to 6.5 and a dissolved oxygen concentration of 1 to 10 ppm is used, the light irradiation intensity is set to 1,000 to 10,000 lux, and the culture of Euglena cells and the protein content in the cells are efficiently performed. A method of culturing Euglena cells that can be enhanced is shown. Patent Literature 6 discloses that water containing at least no radioactive carbon isotope is contained in a water tank to which euglena (Euglena) is irradiated with light suitable for photosynthesis, and carbon dioxide containing no radioactive carbon isotope in the water is disclosed. And a method for producing an experimental euglena that does not contain carbon isotopes for growing euglena.

On the other hand, it has been practiced since ancient times to use drinking water with the magnetic field of a permanent magnet to promote health. For example, a natural magnetite lump was put into well water, left for a while, and then the drink was used to help treat disease. The structure of water is composed of conjugates (clusters) where water molecules are attracted to each other by hydrogen, and a cluster of tap water is made up of, for example, an average of 12 water molecules. Water has the property of being activated under the influence of magnetism (magnetic force). For this reason, when tap water is passed through a magnetic field in which a magnetic force is acting, for example, it is composed of, for example, an average of six water molecule clusters. There are reports that magnetized water is generated.

The present inventor has paid attention to the fact that the earth itself is a magnetic substance, and a magnetic field rolls in outer space with its rotation, and in such an environment, living things have a compass-like function of responding to geomagnetism. For example, as a magnetic sensing bacterium was discovered in 1960, a magnetic bacterium retains magnetite microparticles in the body. It has been found that magnetic bacteria that inhabit the northern hemisphere behave using the south pole as an index, and magnetic bacteria that inhabit the southern hemisphere use the north pole as an index. Then, it can be considered that the intrinsic magnetic material is involved in the biological functions of humans and animals.

装置 An apparatus for producing magnetized water by applying magnetism to water is known. For example, a magnetized water dispenser (see Patent Document 7) including a holder member having an insertion hole through which a pipe through which a fluid flows can be inserted, a plurality of magnetic field generators incorporated in the holder member, and a plurality of magnetization chambers are provided. At the same time, an inner casing forming an inner magnetized flow path is formed by connecting the plurality of magnetized chambers by a passage, and a magnetizer combining a permanent magnet and a yoke plate magnetically attracting the permanent magnet is arranged in the magnetized chamber. By covering the outside of the inner casing with the outer casing, the magnetized water flowing out of the inner casing can be implemented, and an external magnetized flow path that receives the magnetizing action again is formed in the meantime, so that the magnetized water acts on the water flowing through the conduit. A magnetizing device (see Patent Document 8), a magnetic block magnetized magnetically around a water pipe of a water pipe or a supply pipe is positioned and angled in the flow direction of water. (See Patent Document 9), in which the water passing therethrough is swirled by the magnetic force of the magnetic block into magnetized water (see Patent Document 9), and the right and left halves are pivotally connected to open and close. There has been proposed a magnetized water producing device (see Patent Document 10) provided with a connecting portion for freely connecting, and a left super strong magnet slidably provided inside the right half.

JP 60-196184 A JP-A-61-37091 JP-A-61-40785 Japanese Patent No. 2777418 Japanese Patent Publication No. 5-32020 JP 2012-44969 A JP 2005-169358 A JP 2005-40694 A JP-A-11-165278 JP 2003-24949 A JP-A-2005-195793

However, in the conventional magnetized water dispenser described above, the permanent magnet is fixed, the generated magnetic field is weak, and when a magnetic body such as an iron pipe is used as the pipe, the magnetic lines of force are smaller in the pipe having smaller magnetic resistance. The magnetic field acting on the water was weakened, and the effect of magnetizing the water was not sufficient.

Focusing not only on the physical change of water when a magnetic field is applied to water, but also on chemical changes such as active hydrogen contained in water, active hydrogen in an environment where the S and S poles of the permanent magnet are opposed to each other. Is easy to exist stably. Here, active hydrogen is an atomic hydrogen atom, which easily combines with active oxygen and turns into water, which has the effect of removing active oxygen, and as a result, it can work to promote health It has been reported.

When magnetized water is used as drinking water, the intestinal absorption rate generally increases and the viscosity increases, so that the activity of reducing the activity of malignant bacteria such as bacteria is reduced. And the effect of increasing the freshness of vegetables.

Water contains hydrogen ions (H ⁺ ) and hydroxyl ions (OH ⁻ ). Hydrogen ions receive electrons from hydroxyl ions and easily generate atomic hydrogen. It is very unstable because it comes into water and hydrogen ions when it comes into contact with dissolved oxygen in water and cations such as calcium, sodium and magnesium. However, in an environment in which the S and S poles are opposed to each other when atomic hydrogen is generated, atomic hydrogen may be called a magnetic atom whose magnetic moment direction is not determined. In an environment where the poles and the south pole are opposed to each other, the direction of the active magnetic moment of the active hydrogen is not determined, but the active hydrogen fluctuates in water. As a result, the active hydrogen is a stable environment, It has been reported that a large amount of active hydrogen is present in water, and that the effect of promoting the health of magnetized water is exhibited.

In view of the above-described problems of the related art, the present inventor has previously proposed a method and apparatus for growing Euglena by using magnetized water storage or magnetized water as supply water (see Patent Document 11). .). That is, by passing an alternating magnetic field (alternating magnetic flux) directly to the Euglena cells or to the stored water or the supply water, a magnetic field that exerts a good effect on the function of the living body of the Euglena is provided, and the proliferation is promoted. . An object of the present invention is to further increase the productivity of Euglena by supplying oxygen and carbon dioxide gas to the euglena aquaculture tank and providing an alternating magnetic field throughout the aquaculture plant.

For this reason, the euglena aquaculture plant of the present invention, a rotating body equipped with an electromagnetic derivative composed of a plurality of permanent magnets, in order to create an alternating magnetic field in the aquaculture reservoir where Euglena is present or a supply channel to the aquaculture reservoir. A magnetizing device including a motor that rotates the rotating body is mounted, and a magnetic conduction band electrically connected to the magnetizing device is arranged in the culture water tank and the water supply passage, and The first feature is that the aquaculture tank or the supply water is provided with aeration means, and the aquaculture tank is provided with an oxygen gas mixing means for supplying oxygen gas and a carbon dioxide gas mixing means for supplying carbon dioxide gas. .

{Circle around (2)} The second feature is that the magnetizing device is configured such that a plurality of permanent magnets are arranged in a flat plate such that the N pole and the S pole have the same phase or alternate phase.

Further, a third feature is that the driving energy of the magnetizing device is a commercial power source or a power source utilizing natural energy such as solar power generation, hydroelectric power generation, and wind power generation.

Further, a fourth feature is that an aeration water jet nozzle provided with a plurality of permanent magnets and a static mixer is provided.

In addition, the fifth feature is that the aeration means of the aquaculture tank is a water wheel.

With the above configuration, an alternating magnetic field (alternating magnetic flux) is transmitted directly to the Euglena cells or to the stored water or the supply water, thereby providing a magnetic field that exerts a good effect on the biological functions of the Euglena, and oxygen gas. By providing carbon dioxide gas, the photosynthesis of Euglena can be promoted and dissolved oxygen can be increased to inhibit the growth environment of anaerobic microorganisms and improve the growth environment of Euglena.

The present invention applies to any species of the genus Euglena, such as Euglena gracilis, Euglena viride, Euglena intermia, and the like. Further, in the present invention, purely proliferated Euglena cells can be used as seed cells.

It is a partial perspective side view showing typically the basic composition of the Euglena culture plant concerning the present invention. FIG. 1 is a perspective view showing a basic configuration of a magnetizing device used in the present invention. FIG. 2 is a perspective view of a magnetizing device used in the present invention. 3A is a plan view and FIG. 3B is a side view showing an example of the arrangement of permanent magnets in a rotating body of the apparatus in FIG. 3. It is a side view which shows typically one Embodiment of the culture water tank of the Euglena breeding plant which concerns on this invention. It is a schematic diagram which shows the structure of the water pipe of the Euglena aquaculture plant concerning this invention, and a metal magnetic conduction zone. It is a longitudinal cross-sectional view which shows the intake pipe of the aeration apparatus in a supply water storage tank. It is a perspective view which shows the aeration water ejection nozzle used by this invention. (A) is a longitudinal sectional view of an aeration water jet nozzle, and (b) is a transverse sectional view.

In the present invention, by rotating a flat disk-shaped rotating body on which a permanent magnet having a strong magnetic force is arranged at a high speed, N and S alternating magnetic fields can be produced and applied in raw water (for example, tap water or well water). .

(4) As the permanent magnet, it is preferable to use, for example, a surface of 3000 gauss or more of a neodymium magnet, a samarium magnet, or the like. The rotating body of the magnetizing device is rotated at several hundred rpm or more.

[Example]
FIG. 1 shows an embodiment of an Euglena aquaculture plant according to the present invention. FIG. 2 shows the basic principle configuration of the magnetizing device, and FIGS. 3 and 4 show specific embodiments.

Figure 1 shows the Euglena aquaculture plant. Euglena cells are introduced into the culture tank 19 in which the source water W is stored. Then, water or liquid fertilizer can be supplied from the supply water storage tank 11 to the culture water storage tank 19 via the water supply pipe 11a. In the middle of the water supply pipe 11a and on the left and right side surfaces of the culture water tank 19, a pair of metal conductive bands 8 electromagnetically connected to a pair of electromagnetic derivatives (metal rods) 4 of the magnetizing device 10 by a conducting wire 7 will be described. It is arranged so as to sandwich the upper and lower sides or the left and right sides, so that an alternating magnetic field / pulse magnetic field and vibration imparting water can be generated. An aeration water jet nozzle 9 is attached to the tip of the water supply pipe 11a so that magnetized water AW containing microbubbles can be supplied to the aquaculture storage tank 19. In FIG. 1, reference numeral 13 denotes an arbitrary aerator, 14 denotes a motor for intake and water discharge, and 15 denotes an intake pipe.

The magnetizing device 10 used in the present invention includes a flat disk-shaped rotating body 1 and a motor 2 connected to the rotating body 1 via a rotating shaft 2a. The magnet 1a is fixed at an arbitrary interval. A pair of electromagnetic conductors (metal rods) 4 extend so as to sandwich the rotating body 1 from above and below, and transmit an alternating magnetic field / pulse magnetic field and a wave generated from the rotating body 1 to an object (for example, water storage). Have been able to.

The permanent magnets 1a are arranged at equal intervals along the circumference of the rotating body 1. The magnets 1a may be arranged such that N poles and S poles are alternately located, or may be arranged such that the same poles are adjacent to each other. The number of arrangements is also arbitrary. The rotating body 1 rotates at high speed by a rotating shaft 2a connected to a motor 2. In the present embodiment, the power source of the motor 2 is the commercial power source 3, but it is needless to say that it is useful to use a natural power source such as sunlight, hydraulic power, and wind power.

FIG. 3 shows a compact realization of the magnetizing device 10, in which a motor body 2 and a rotating body 1 are housed in a cylindrical body 5b made of a punched metal plate, and a main body cover 5 covered with an upper lid 5a is provided. The magnetizing device 10 is attached to the character-shaped base 6 so as to be tiltable. As shown in FIGS. 4 and 6, a pair of electromagnetic conductors (metal rods) 4 of the magnetizing device 10 are connected to a water supply pipe 11 a sandwiched by a pair of metal conductive bands 8 via a conducting wire 7. To obtain an alternating magnetic field, a pulsed magnetic field, and a wave imparting water.

As shown in FIG. 5, a pair of metal conductive bands 8 described above are arranged along the length direction of the culture water storage tank 19 to apply an alternating magnetic field to the water storage and a plurality of stirring blades 17 are provided. A water wheel 16 provided is attached, and is rotated by the water supplied from the aeration water jet nozzle 9 to agitate the culture tank 19 to further aerate and generate flowing water. Further, an oxygen gas mixing pipe 20 and a carbon dioxide gas mixing pipe 21 are provided at the bottom of the aquaculture storage tank 19, and oxygen gas and carbon dioxide gas are bubbled and supplied from the

respective fumaroles

20a and 21a into the storage water. In addition, it promotes euglena photosynthesis, increases dissolved oxygen concentration, and suppresses the growth of anaerobic microorganisms.

Here, it is preferable in terms of the natural environment to use carbon dioxide gas emitted from factories, vehicles and the like. Incidentally, a motor may be used as a drive source of the water wheel 16. The growth water temperature of Euglena is preferably 16 ° C. to 30 ° C., and the temperature is preferably controlled by a radiator or a magnetic water heater. It is also important to appropriately adjust the light receiving rate of Euglena using a cold gauze or the like according to the sunshine rate that fluctuates every four seasons.

As shown in FIG. 7, a plurality of permanent magnets 1a are arranged around intake ports 15a at both ends of the intake pipe 15 of the supply water tank 11, and the sucked atmosphere A is sent to the aeration device 13. . The aeration device 13 takes in the air A sucked from the suction part 5 and generates gas-liquid mixed water AW in which bubbles are mixed and magnetized.

In the aeration device 13, although not shown, bubbles generated in the flowing water increase in flow velocity when passing through the tapered pipe, are decompressed and expanded, and thereafter are compressed and contracted as the cross-sectional area increases. However, the bubbles are fragmented by the shearing force generated at this time, and fine bubbles are generated.

In addition, as the air suction portion, a system in which air is sucked toward a negative pressure generated in a static impeller can be used. In addition to the above-described embodiment, air is sucked from a hole of a cylindrical pipe along the impeller. The gas vent hole is drilled in the back of a hollow box-shaped impeller fixed to the pipe vent and the ventilator, and the pipe is spirally attached to the cylindrical pipe along the back in the rotation direction of the impeller attached spirally to the pipe. A device equipped with a gas outlet can also be used.

自 Such a self-priming suction unit can generate a large amount of air A as extremely fine bubbles in flowing water. That is, a strong vacuum pressure is generated in the flowing water, and the air can be sucked into the flowing water with small energy. Further, since the air is miniaturized, the dissolution efficiency of oxygen can be increased.

Further, as shown in FIGS. 8 and 9, a plurality of permanent magnets 1a and a static mixer 13 for creating an alternating magnetic field of N and S are attached to the supply water, and the air is sucked from the air inlet 9a. An aeration water jet nozzle 9 for generating fine bubbles by cavitation action may be attached to the outside of the reservoir. In this case, the dissolved oxygen can be supplemented by aeration at the same time as the magnetization of the water, and it is particularly useful as a nozzle for supplying water to the aquaculture storage tank. The aeration may be performed by using a propeller (not shown) instead of the static mixer 13, and an intended effect of discharging a water flow containing fine bubbles can be obtained.

プラント The plant of the present invention is highly practical and extremely useful especially in Euglena breeding production.

DESCRIPTION OF SYMBOLS 1 Rotating body 1a Permanent magnet 2 Motor 2a Motor rotating shaft 3 Commercial power supply or natural energy use power supply 4 Electromagnetic derivative (metal rod)
Reference Signs List 5 Body cover 5a Lid 5b Cylindrical body 6 Stand 7 Conductor wire 8 Magnetic conduction band (metallic band)
Reference Signs List 9 aeration water jet nozzle 9a intake hole 10 magnetizing device 11 supply water reservoir 11a water supply pipe 12 static mixer 13 aeration device 14 motor for intake and water discharge 15 intake pipe 15a intake port 16 water wheel 17 stirring blade 18 euglena harvest nozzle 19 culture water Vessel 20 Oxygen gas mixing tube 20a Oxygen gas blowing hole 21 Carbon dioxide gas mixing tube 21a Carbon dioxide gas blowing hole W Source water (supply water)
A Air (atmosphere)
AW Aerated magnetized water U Euglena

Claims

In order to create an alternating magnetic field in the aquaculture water tank in which Euglena is present or a supply channel to the aquaculture water tank, a rotating body provided with an electromagnetic derivative composed of a plurality of permanent magnets, and a motor that rotates the rotating body Along with mounting the configured magnetizing device, a magnetic guiding zone electrically connected to the magnetizing device is disposed in the culture water tank and the water supply passage, and aeration means is provided for the culture water tank and the supply water, respectively. A cultivation plant for Euglena, comprising: an oxygen gas mixing means for supplying oxygen gas to the aquaculture storage tank; and a carbon dioxide gas mixing means for supplying carbon dioxide gas.
2. The euglena aquaculture plant according to claim 1, wherein the magnetizing device comprises a plurality of permanent magnets arranged in a flat plate such that the N pole and the S pole have the same phase or alternate phase.
The euglena aquaculture plant according to claim 1 or 2, wherein the driving energy of the magnetizing device is a commercial power source or a power source utilizing natural energy such as solar power generation, hydroelectric power generation, and wind power generation.
The Euglena aquaculture plant according to any one of claims 1 to 3, further comprising an aeration water jet nozzle provided with a plurality of permanent magnets and a static mixer.
The euglena aquaculture plant according to any one of claims 1 to 4, wherein the aeration means of the aquaculture tank is a water wheel.