JPWO2020044499A1 - Euglena aquaculture plant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To promote photosynthesis of Euglena by imparting a magnetic field which exerts a good effect on the function of a living body of Euglena and to impart oxygen and carbon dioxide gas, and to increase dissolved oxygen to generate anaerobic microorganisms. Inhibits and improves the growing environment of Euglena. SOLUTION: In order to create an alternating magnetic field in a water supply channel 11a to a culture water storage tank 19 in which Euglena exists, a rotating body 1 having an electromagnetic derivative composed of a plurality of permanent magnets 1a and the rotating body 1 are rotated. A magnetization device 10 composed of a motor 2 is mounted, and a magnetic guide band 8 electrically connected to the magnetization device 10 is arranged in the culture water storage tank 19 and the water passage 11a of the supply water, respectively, and the culture water storage tank 19 is provided. Alternatively, each supply water is provided with an aeration means, and an oxygen gas mixing means 20 for supplying oxygen to the culture water storage tank 19 and a carbon dioxide gas mixing means 21 for supplying carbon dioxide gas are provided.

Description

The present invention relates to a euglena aquaculture plant, and more particularly to a euglena aquaculture plant capable of efficiently increasing the production of euglena by magnetizing and aerating the stored water or supply water in which euglena is present.

Euglenida is a plant classified in the phylum Euglena, also a protozoa, an animal belonging to the suborder Flagellate, and a protist belonging to both animals and plants. Euglena is a unicellular eukaryote that is taxonomically classified into both plant and animal categories, Euglena in the plant category, Euglena algae in the plant category, and in the animal category, flagellates in the protozoa category. Belongs to. Euglena has been reported to be more nutritious than the algae chlorella and spirulina because it is composed of excellent animal proteins, has an amino acid balance close to that of the milk protein casein, and contains moderate amounts of vitamins and inorganic salts. There is.

Euglena grows under various growth conditions, and various useful substances contained in its components contain polyunsaturated fatty acids that are not synthesized in the human body. .. For example, it is cultivated by a batch culture method using a culture flask, a culture tank, etc., but is generally grown by controlling only the temperature and the initial pH, and without controlling dissolved oxygen, light irradiation intensity, stirring, etc. ..
For this reason, productivity and quality are insufficient, and while they have excellent food value, pharmaceutical materials, and potential value as fuel manufacturing materials, they have not been effectively used.

For example, Patent Document 1 states that when euglena is cultured, the production and secretion of waxy substances and mucilages are suppressed by aerating high concentrations of oxygen, and the synthesis of nutritional components such as unsaturated fatty acids and vitamin E is performed. A method of culturing Euglena for the purpose of promoting it is shown. Further, in Patent Document 2, the carbon concentration and the nitrogen concentration are adjusted and supplied, and the Euglena cells are continuously cultured, so that the same culture time is used as compared with the case where the Euglena cells are cultured by the conventional batch culture method. It has been shown that 5 to 10 times as many Euglena cells can be obtained, and that the intracellular protein content of Euglena cells is similar to that of the conventional one.

Furthermore, Patent Document 3 (Japanese Unexamined Patent Publication No. 61-40785) discloses a method for culturing Euglena cells, which is a combination of culturing under closed system darkness and culturing under open system light irradiation. In Patent Document 4, the gas component necessary for culturing is ejected and supplied from below the culturing tank toward the periphery of the bottom surface of the culturing tank, and the culture broth rotates along the inner wall of the culturing tank in the radial direction from the rotation axis. Stirring is performed by a stirring rotary blade composed of a protruding radiation blade and an inclined blade having a larger area than the radiation blade and inclined at an angle of 35 to 55 ° with respect to the radiation blade. The method of culturing Euglena and the culturing apparatus are shown.
Then, according to the device, the vertical stirring is naturally configured in which the flow of the growth liquid rises so as to swirl around the inner wall surface of the culture tank by the rotary blade for stirring and descends along the circumference of the rotation axis. .. In addition, since the algae that tend to concentrate around the bottom surface of the culture tank are dispersed by the jet output of gas, they can be dispersed without giving mechanical sure stress. Then, the algae dispersed by the gas jet output are swirled by the rotating blades, rise along the inner wall of the tank, and fall near the axis, so that the diffusion to the entire tank is smoothly performed, and the overweight consumption of nutrients is avoided. Is shown.

In Patent Document 5, a medium having a pH of 1.8 to 6.5 and a dissolved oxygen concentration of 1 to 10 ppm is used, the light irradiation intensity is set to 1000 to 10000 lux, and the culture of Euglena cells and the protein content in the cells are efficiently controlled. A method of culturing Euglena cells that can be enhanced has been shown. In Patent Document 6, at least water containing no radiocarbon isotope is contained in a water tank irradiated with light suitable for photosynthesis by Euglena, and carbon dioxide containing no radiocarbon isotope is contained in the water. A method for producing experimental Euglena that does not contain carbon isotopes that grow Euglena is shown.

On the other hand, it has been practiced since ancient times to apply the magnetic field of a permanent magnet to drinking water to help improve health. For example, a mass of natural magnetite was put into well water, left for a while, and then drunk to help treat illness. The structure of water consists of bonds (clusters) in which water molecules are attracted to each other by hydrogen, and a cluster of tap water is composed of, for example, an average of 12 water molecules. Water has the property of being activated when it is affected by magnetism (magnetic force). Therefore, when tap water is passed through a magnetic field in which magnetic force is working, it consists of, for example, an average of 6 water molecule clusters. There is a report that magnetized water is generated.

The present inventor has focused on the fact that the earth itself is a magnetic material, and the magnetic field rolls in outer space along with its rotation, and the organism has a compass-like function that reacts to the geomagnetism in those environments. For example, as magnetic sensing bacteria were discovered in 1960, magnetic bacteria retain magnetite microparticles in their bodies. It has been found that the magnetic bacteria inhabiting the northern hemisphere act using the south pole as an index, and the magnetic bacteria inhabiting the southern hemisphere act using the north pole as an index. Then, it can be considered that the magnetic substances inherent in the biological functions of humans and animals are also involved.

A device for producing magnetized water by applying magnetism to water is known. For example, a magnetized water device (see Patent Document 7) including a holder member having an insertion hole through which a pipe through which a fluid flows can be inserted, and a plurality of magnetic field generators incorporated in the holder member, and a plurality of magnetizing chambers are provided. At the same time, an internal casing forming an inner magnetization flow path is formed by communicating these plurality of magnetization chambers through a passage, and a magnetizer combining a permanent magnet and a yoke plate that magnetically attracts the magnetizer is arranged in the magnetization chamber, and the inner casing is provided. By covering the outside of the outer casing with an outer casing, the magnetized water flowing out of the inner casing is fulfilled, and an outer magnetized flow path that is magnetized again is formed between them, so that the water flowing through the conduit is magnetized. Magnetized devices (see Patent Document 8), magnetic blocks magnetized with magnetic force around the water passages of water pipes and supply pipes are arranged at different positions and angles in the water flow direction, and the passing water is magnetically blocked. (See Patent Document 9), which is made into magnetized water by swirling with the magnetic force of (see Patent Document 9). On the other hand, a magnetized water maker (see Patent Document 10) equipped with a left super-strong magnet installed so as to be slidable has been proposed.

Japanese Unexamined Patent Publication No. 60-196184 Japanese Unexamined Patent Publication No. 61-37091 Japanese Unexamined Patent Publication No. 61-40785 Japanese Patent No. 2777418 Special Fair 5-3020 Japanese Unexamined Patent Publication No. 2012-44969 Japanese Unexamined Patent Publication No. 2005-169358 Japanese Unexamined Patent Publication No. 2005-40964 Japanese Unexamined Patent Publication No. 11-165278 Japanese Unexamined Patent Publication No. 2003-24949 Japanese Unexamined Patent Publication No. 2015-195793

However, in the above-mentioned conventional magnetized water treatment device, when the permanent magnet is fixed, the generated magnetic field is weak, and a magnetic material such as an iron pipe is used as the pipe, the magnetic field line is the pipe having a small magnetic resistance. The magnetic field acting on water was weakened, and the effect of magnetizing water was not sufficient.

Focusing not only on the physical changes in water when a magnetic field is applied to water, but also on the chemical changes such as active hydrogen contained in water, active hydrogen in an environment where the south and south poles of a permanent magnet are opposed to each other. Is likely to exist stably. Here, active hydrogen is an atomic hydrogen atom that easily combines with active oxygen and turns into water, which is effective in removing active oxygen, and as a result, it can work to improve health. It has been reported.

When magnetized water is used as drinking water, the absorption rate of the intestine generally increases and the viscosity increases, so the effect of reducing the activity of malignant bacteria such as bacteria, and the use of magnetized water for washing vegetables etc. has a water retention effect. It also has the effect of increasing the freshness of vegetables.

Water contains hydrogen ions (H ⁺ ) and hydroxide ions (OH ⁻ ). Hydrogen ions receive electrons from hydroxide ions and easily generate atomic hydrogen, but the generated active hydrogen is It is extremely unstable because it becomes water or hydrogen ions when it comes into contact with dissolved oxygen in water and cations such as calcium, sodium, and magnesium. However, if the S pole and the S pole are opposed to each other when the atomic hydrogen is generated, it can be said that the atomic hydrogen is a magnetic atom whose direction of the magnetic moment is not fixed. In an environment where the pole and the south pole are opposed to each other, the direction of the magnetic moment of active hydrogen is not fixed and it moves flutteringly in water, but as a result, it is a stable environment for active hydrogen, and this is magnetization. It has been reported that as a result of the presence of a large amount of active hydrogen in water, the effect of promoting the health of magnetized water is exhibited.

Therefore, in view of the above-mentioned problems of the prior art, the present inventor has previously proposed a method and an apparatus for growing Euglena by storing magnetized water or using magnetized water as supply water (see Patent Document 11). .). That is, by allowing Euglena cells to transmit an alternating magnetic field (alternate magnetic flux) directly or through stored water or supply water, a magnetic field that exerts a good effect on the function of the living body of Euglena is imparted and its proliferation is planned. .. An object of the present invention is to further improve the production of Euglena by supplying oxygen and carbon dioxide gas to Euglena's culture water tank and providing alternating magnetic fields throughout the culture plant.

Therefore, the Euglena culture plant of the present invention includes a rotating body provided with an electromagnetic derivative composed of a plurality of permanent magnets in order to create an alternating magnetic field in the culture water tank in which Euglena exists or the water supply channel to the culture water tank. A magnetizing device composed of a motor for rotating the rotating body is mounted, and a magnetic guiding band electrically connected to the magnetizing device is arranged in the culture water storage tank and the water passage of the supply water, respectively. The first feature is that each of the culture water storage tank or the supply water is provided with an aeration means, and the culture water storage tank is provided with an oxygen gas mixing means for supplying oxygen gas and a carbon dioxide gas mixing means for supplying carbon dioxide gas. ..

The second feature of the magnetization device is that a plurality of permanent magnets are arranged in a flat plate shape so that the north and south poles are in the same phase or in alternating phases.

Furthermore, the third feature is that the driving energy of the magnetizing device is a commercial power source or a power source using natural energy such as solar power generation, hydroelectric power generation, and wind power generation.

Further, the fourth feature is that it is provided with an aerated water ejection nozzle equipped with a plurality of permanent magnets and a static mixer.

In addition, the fifth feature is that the aeration means of the aquaculture water tank is a water wheel.

With the above configuration, by allowing Euglena cells to transmit an alternating magnetic field (alternate magnetic field) directly or through their stored water or supply water, a magnetic field that exerts a good effect on Euglena's biological functions is imparted, and oxygen gas is also provided. And by applying carbon dioxide gas, it is possible to promote the photosynthesis of Euglena and increase the dissolved oxygen to inhibit the growth environment of anaerobic microorganisms and improve the growth environment of Euglena.

The present invention applies to any species of the genus Euglena, such as Euglena gracilis, Euglena billide, Euglena intermedia. Further, in the present invention, purely proliferated Euglena cells can be used as seed cells.

It is a partial perspective side view which shows typically the basic structure of the Euglena aquaculture plant which concerns on this invention. It is a perspective view which shows the basic structure of the magnetization device used in this invention. It is a perspective view of the magnetization device used in this invention. FIG. 3A is a plan view and FIG. 3B is a side view showing an example of arrangement of permanent magnets in the rotating body of the device of FIG. It is a side view which shows typically one Embodiment of the aquaculture water tank of the Euglena breeding plant which concerns on this invention. It is a schematic diagram which shows the structure of the water pipe and the metal conductive band of the Euglena culture plant which concerns on this invention. It is a vertical cross-sectional view which shows the intake pipe of the aeration device in a supply water water storage tank. It is a perspective view which shows the aeration water ejection nozzle used in this invention. (A) is a vertical cross-sectional view of the aerated water ejection nozzle, and (b) is a cross-sectional view.

In the present invention, an alternating magnetic field of N and S can be created and applied in raw water (for example, tap water or well water) by rotating a flat plate-shaped rotating body in which a permanent magnet having a strong magnetic force is arranged at high speed. ..

As the permanent magnet, for example, it is preferable to use a neodymium magnet, a samarium magnet, or the like with a surface of 3000 gauss or more as a reference. Further, the rotating body of the magnetization device is rotated at several hundred rpm or more.

[Example]
FIG. 1 shows an example of the Euglena aquaculture plant according to the present invention. FIG. 2 shows the basic principle configuration of the magnetization device, and FIGS. 3 and 4 show specific embodiments.

FIG. 1 shows a Euglena aquaculture plant. Euglena cells are put into the aquaculture water tank 19 in which the source water W is stored. Then, water or liquid fertilizer can be supplied from the water supply water storage tank 11 to the aquaculture water storage tank 19 via the water supply pipe 11a. A pair of metal conductive bands 8 electromagnetically connected to a pair of electromagnetic derivatives (metal rods) 4 of a magnetization device 10 described later by a conducting wire 7 are formed in the middle of the water supply pipe 11a and on the left and right side surfaces of the culture water storage tank 19. It is arranged so as to sandwich the top and bottom or left and right so that an alternating magnetic field, a pulse magnetic field, and vibration-applied water can be generated. Further, an aerated water ejection nozzle 9 is attached to the tip of the water supply pipe 11a so that the magnetized water AW containing fine bubbles can be charged into the aquaculture water tank 19. In FIG. 1, 13 is an arbitrary aeration device, 14 is a motor for intake and water discharge, and 15 is an intake pipe.

The magnetizing device 10 used in the present invention includes a flat plate-shaped rotating body 1 and a motor 2 connected to the rotating body 1 via a rotating shaft 2a, and a plurality of permanent magnets are inside the rotating body 1. The magnets 1a are fixed at arbitrary intervals. Then, a pair of electromagnetic derivatives (metal rods) 4 are extended so as to sandwich the upper and lower sides of the rotating body 1, and the alternating magnetic field, the pulse magnetic field, and the wave generated from the rotating body 1 are transmitted to the object (for example, water storage). It is made possible.

The permanent magnets 1a are arranged at equal intervals along the circumference of the rotating body 1. The magnets 1a may be arranged so that the north and south poles are alternately positioned, or the same poles may be arranged so as to be adjacent to each other. The number to be arranged is also arbitrary. The rotating body 1 rotates at high speed by the rotating shaft 2a connected to the motor 2. Although the power source of the motor 2 is the commercial power source 3 in this embodiment, it goes without saying that it is beneficial to use the energy of the natural world such as solar power, hydraulic power, and wind power as the power source.

FIG. 3 shows a compact embodiment of the magnetization device 10, which includes a main body cover 5 in which a motor 2 and a rotating body 1 are housed in a cylinder main body 5b made of a punching metal plate and covered with an upper lid 5a. The magnetization device 10 is tiltably attached to the character-shaped mount 6. Then, as shown in FIGS. 4 and 6, the pair of electromagnetic derivatives (metal rods) 4 of the magnetization device 10 is connected to the water supply pipe 11a sandwiched between the pair of metal conductive bands 8 via the conducting wire 7. To obtain an alternating magnetic field, a pulse magnetic field, and wave-imparting water.

As shown in FIG. 5, in the culture water storage tank 19, the pair of metal conductive bands 8 described above are arranged along the length direction thereof to apply an alternating magnetic field to the water storage, and a plurality of stirring blades 17 are provided. The provided water wheel 16 is attached and rotated by the water supply discharged from the aerated water ejection nozzle 9 to agitate the inside of the culture water storage tank 19 to further aerate and generate running water. Further, an oxygen gas mixing pipe 20 and a carbon dioxide gas mixing pipe 21 are arranged at the bottom of the culture water storage tank 19, and oxygen gas and carbon dioxide gas are bubbled into the water storage and supplied from the respective jet holes 20a and 21a. It promotes photosynthesis of Euglena, increases the concentration of dissolved oxygen, and suppresses the growth of anaerobic microorganisms.

Here, it is preferable to use carbon dioxide gas discharged from a factory, a vehicle, or the like in terms of the natural environment. A motor may be used as the drive source of the water turbine 16. The growth water temperature of Euglena is preferably 16 ° C. to 30 ° C., and the temperature is preferably controlled by a radiator or a magnetic water heater. It is also important to appropriately adjust the light receiving rate of Euglena using a lawn cloth or the like according to the sunshine rate that fluctuates from season to season.

As shown in FIG. 7, a plurality of permanent magnets 1a are arranged around the intake ports 15a at both ends in the intake pipe 15 of the supply water storage tank 11, and the sucked air A is sent to the aeration device 13. .. The aeration device 13 takes in the air A sucked from the intake unit 5 and mixes bubbles to generate a magnetized gas-liquid mixed water AW.

In the aeration device 13, although not particularly shown, when air bubbles generated in running water pass through the tapered tube, the flow velocity increases and the pressure is reduced to expand, and then the air bubbles are pressurized and contracted as the cross-sectional area increases. However, the structure is such that the bubbles are subdivided by the shearing force generated at this time and fine bubbles are generated.

As the air intake part, a method in which air is sucked toward the negative pressure generated in the static impeller can also be used. In addition to the above embodiment, air is sucked from the hole of the cylindrical pipe along the impeller. A pipe vent and a hollow box-shaped impeller fixed to be ventilated with a gas ejection hole in the back, and a cylindrical pipe spirally attached to the pipe along the back of the impeller in the direction of rotation. Those equipped with a gas outlet can also be used.

Such a self-priming intake unit can generate a large amount of air A as ultrafine bubbles in running water. That is, a strong vacuum pressure can be generated in the running water, and the atmosphere can be sucked into the running water with a small amount of energy. Further, since the air is made extremely fine, the oxygen dissolution efficiency can be improved.

Further, as shown in FIGS. 8 to 9, a plurality of permanent magnets 1a and a static mixer 13 for creating an alternating magnetic field of N and S are attached to the supplied water, and the air is sucked from the intake hole 9a. An aerated water ejection nozzle 9 that generates fine bubbles by a cavitation action may be attached to the outside of the water storage. In this case, aeration can be performed at the same time as the magnetization of water to supplement the dissolved oxygen, which is particularly useful as a nozzle for supplying water to the aquaculture water tank. It should be noted that aeration may be performed using a propeller (not shown) instead of the static mixer 13, and the desired effect of discharging a fine bubble water-containing stream can be obtained.

The plant of the present invention is highly practical and extremely useful, especially in Euglena breeding production.

1 Rotating body 1a Permanent magnet 2 Motor 2a Motor rotating shaft 3 Commercial power supply or natural energy utilization power supply 4 Electromagnetic derivative (metal rod)
5 Body cover 5a Lid 5b Cylinder 6 Stand 7 Conductor 8 Conductor band (metal band)
9 Exposed water ejection nozzle 9a Intake hole 10 Magnetizing device 11 Supply water storage tank 11a Water supply pipe 12 Static mixer 13 Air exposure device 14 Intake and discharge motor 15 Intake pipe 15a Intake port 16 Water wheel 17 Stirring blade 18 Euglena harvest nozzle 19 Cultured water storage Tank 20 Oxygen gas mixing pipe 20a Oxygen gas injection hole 21 Carbon dioxide gas mixing pipe 21a Carbon dioxide gas injection hole W Source water (supply water)
A Air (atmosphere)
AW Aeration Magnetized Water U Euglena

Claims (5)

From a rotating body equipped with an electromagnetic derivative composed of a plurality of permanent magnets and a motor that rotates the rotating body in order to create an alternating magnetic field in the cultured water tank in which Euglena exists or the water supply channel to the cultured water tank. Along with mounting the configured magnetizing device, a magnetic guiding band electrically connected to the magnetizing device is arranged in each of the culture water storage tank and the water passage of the supply water, and aeration means is provided to the culture water storage tank or the supply water, respectively. A Euglena culture plant characterized in that an oxygen gas mixing means for supplying oxygen gas and a carbon dioxide gas mixing means for supplying carbon dioxide gas are provided in the culture water storage tank. The Euglena cultivation plant according to claim 1, wherein the magnetizing device arranges a plurality of permanent magnets in a flat plate shape so that the north and south poles are in the same phase or in alternating phases. The Euglena cultivation plant according to claim 1 or 2, wherein the driving energy of the magnetizing device is a commercial power source or a power source using natural energy such as solar power generation, hydroelectric power generation, and wind power generation. The Euglena cultivation plant according to any one of claims 1 to 3, further comprising an aerated water ejection nozzle provided with a plurality of permanent magnets and a static mixer. The Euglena aquaculture plant according to any one of claims 1 to 4, wherein the aeration means of the aquaculture water tank is a water wheel.

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