WO2020004388A1 - Dispositif de culture, unité de culture, et procédé de collecte de cible de culture - Google Patents

Dispositif de culture, unité de culture, et procédé de collecte de cible de culture Download PDF

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Publication number
WO2020004388A1
WO2020004388A1 PCT/JP2019/025158 JP2019025158W WO2020004388A1 WO 2020004388 A1 WO2020004388 A1 WO 2020004388A1 JP 2019025158 W JP2019025158 W JP 2019025158W WO 2020004388 A1 WO2020004388 A1 WO 2020004388A1
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WIPO (PCT)
Prior art keywords
carrier
culture
unit
roller
support
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PCT/JP2019/025158
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English (en)
Japanese (ja)
Inventor
幹夫 都筑
万里 岩越
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日本曹達株式会社
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Publication of WO2020004388A1 publication Critical patent/WO2020004388A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/14—Apparatus for enzymology or microbiology with means providing thin layers or with multi-level trays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/16—Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/16—Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
    • C12M1/18—Multiple fields or compartments
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Definitions

  • the present invention relates to a culture device, a culture unit, and a method for collecting a culture target.
  • a culture device a culture unit
  • a method for collecting a culture target Priority is claimed on Japanese Patent Application No. 2018-12460 filed on June 27, 2018, the content of which is incorporated herein by reference.
  • Microorganisms such as microalgae and photosynthetic bacteria, such as Chlorella, CO 2 are very promising as a resource resources available on other industrial energy can be produced not vent, utilization and efficient on a commercial level Expectations are being placed on the perfect production.
  • microalgae such as chlorella
  • it is required to produce them at the lowest possible cost.
  • microalgae such as chlorella
  • it is required to produce them at the lowest possible cost.
  • large pools and tanks must be used. I need. Therefore, there are problems such as an increase in cost due to land acquisition or large-scale equipment.
  • the culture solution is allowed to flow down to the carriers arranged in the vertical direction, the microalgae are grown on the carrier, and the microalgae are removed from the culture solution that has flowed down.
  • Culture systems for recovery have been proposed (for example, Patent Documents 1 and 2).
  • the thin water film on the carrier surface corresponds to the pool water surface of the conventional method, and photosynthesis is performed smoothly by obtaining light (artificial light), carbon dioxide, and nutrients.
  • an object of the present invention is to provide a culture device or the like in which the work efficiency for exchanging carriers is improved.
  • a culture target such as a microorganism
  • the culture apparatus includes a carrier unit for culturing a culture target, a holding unit for holding the carrier unit, and a collection unit for collecting a culture target cultured on the carrier unit,
  • the carrier portion is provided on the carrier to which the culture target is attached, a container for accommodating the carrier therein, a support for supporting the container and the carrier inside the container, and the support. And a held part held by the holding part.
  • the support may support the carrier inside the container and have a carrier support for supplying gas into the container.
  • the carrier has a first surface and a second surface provided apart from each other and opposed to each other inside the container, and the culture device is sandwiched between the first surface and the second surface of the carrier.
  • the support may support the container from the inside of the container and have a container support for supplying a culture solution to the carrier.
  • the carrier may have a curved portion that is curved and hung on the support, and the container support may supply a culture solution to the curved portion of the carrier.
  • the container may be formed in a flat plate shape in which a space for accommodating the carrier is formed. It is preferable to have a pair of rollers provided so as to sandwich the carrier, and a moving mechanism for moving the pair of rollers along the surface of the carrier.
  • a culture unit includes a carrier to which a culture target is attached, a container that contains the carrier therein, and a support that supports the container and the carrier inside the container. And a held part provided on the support and held by a holding mechanism of the culture device.
  • a carrier to which a culture target is attached a container for housing the carrier therein, a support for supporting the container and the carrier inside the container, and the support Holding a carrier portion having a held portion provided by the holding mechanism of the culture device and the holding mechanism of the culture device, Culturing a culture target on the carrier, Recovering the culture target from the carrier.
  • the work efficiency of replacing the carrier can be improved, and the temperature near the carrier can be easily maintained at a temperature suitable for culture.
  • FIG. 1 is a schematic perspective view showing a culture system according to the present embodiment. It is a schematic front view of the culture system according to the present embodiment. It is a partial enlarged view of a carrier unit.
  • FIG. 4 is a sectional view taken along the line IV-IV in FIG. 3. It is a front view of a carrier and a cover. It is sectional drawing in Vb-Vb of FIG. 5A. It is an expanded sectional view of a carrier. It is explanatory drawing of a protrusion part.
  • It is a perspective view showing a roller unit.
  • FIG. 7 is a cross-sectional view illustrating an operation of the roller unit. It is an enlarged view in VIIIb of FIG. 8A. It is a flowchart which shows operation
  • FIG. 1 is a schematic configuration diagram showing a culture system 1 according to the present embodiment. First, a schematic configuration of a culture system 1 to which the present embodiment is applied will be described with reference to FIG.
  • a culture system 1 as an example of a culture apparatus includes a carrier unit 10 for attaching and growing microorganisms to a carrier 12, a culture solution and a microorganism to be cultured from the carrier 12 by pressing the carrier unit 10.
  • a roller unit 30 for dropping the medium, a culture solution supply unit 50 for supplying the culture solution to the carrier unit 10 and collecting the culture solution together with the microorganisms from the carrier unit 10, and a gas supply unit for supplying the carrier unit 10 with a culture gas
  • It has an irradiation unit 60 for irradiating the carrier unit 10 with light, a frame 80 for detachably supporting the carrier unit 10, and a control unit 90 for controlling each component.
  • the vertical direction of the culture system 1 shown in FIG. 1 may be simply referred to as the vertical direction.
  • the width direction of the culture system 1 may be simply referred to as the width direction.
  • the direction intersecting the up-down direction and the width direction in the culture system 1 may be simply referred to as the depth direction.
  • FIG. 2 is a schematic front view of the culture system 1 according to the present embodiment. A detailed configuration of the culture system 1 to which the present embodiment is applied will be described with reference to FIGS.
  • the carrier unit 10 which is an example of the carrier unit and the culture unit, includes a carrier 12 to which microorganisms are attached, a cover 15 covering the outer periphery of the carrier 12, and a support 17 supporting the carrier 12 and the cover 15. And
  • microorganisms are cultured in the carrier unit 10 having a generally flat plate shape. Details of the carrier unit 10 will be described later.
  • the roller unit 30 includes a lifting unit 31 that moves up and down along the carrier unit 10 and a driving unit 32 that drives the lifting unit 31.
  • the roller unit 30 separates the microorganisms cultivated in the carrier unit 10 from the carrier 12 and collects the microorganisms together with the culture solution by moving the elevating unit 31 by the driving unit 32. Details of the roller unit 30 will be described later.
  • the culture solution supply unit 50 which is an example of a collection unit and a storage unit, includes a first pipe 51 that supplies a culture solution to the carrier unit 10, a second pipe 53 that collects the culture solution from the carrier unit 10, and a storage medium that stores the culture solution.
  • Tank 55 a third pipe 57 for circulating a culture solution obtained by separating microorganisms from the culture solution stored in the tank 55, and a pump 59 serving as a driving source for circulating the culture solution.
  • the culture solution supply unit 50 supplies the culture solution to the carrier unit 10 by the pump 59, and collects the microorganisms cultured in the carrier unit 10 together with the culture solution.
  • the culture solution supply unit 50 collects the microorganism by separating the culture solution from the culture solution in the tank 55.
  • the culture solution containing the microorganism is separated into a precipitate containing the microorganism at a high concentration and a supernatant containing almost no microorganism.
  • Pump 59 sucks the supernatant.
  • the supply amount of the culture solution per unit time can be adjusted by controlling the pump 59.
  • the gas supply unit 60 has a gas cylinder 61 for compressing and containing carbon dioxide gas, and a gas pipe 63 for supplying carbon dioxide gas supplied from the gas cylinder 61 to the carrier unit 10.
  • the irradiation unit 70 has a first irradiation panel 71 and a second irradiation panel 73 that irradiate light toward the carrier unit 10 from both sides in the depth direction with the carrier unit 10 interposed therebetween.
  • the first irradiation panel 71 and the second irradiation panel 73 have a substantially plate-like shape that is arranged substantially parallel to and opposed to the carrier unit 10.
  • the size of the first irradiation panel 71 and the second irradiation panel 73 is preferably the same size as that of the carrier 12 so as to irradiate a substantially uniform light amount over almost the entire surface of the carrier 12. It is not limited to this as long as it can be equalized.
  • the first irradiation panel 71 and the second irradiation panel 73 include, for example, a fluorescent lamp, an organic EL, an LED, or the like as a light source, and are configured to irradiate light having a wavelength and a light amount suitable for the growth of microorganisms. I have.
  • the wavelength of the light emitted from the first irradiation panel 71 and the second irradiation panel 73 is, for example, in the range of 380 to 780 nm. It is preferable that the first irradiation panel 71 and the second irradiation panel 73 can irradiate only red light to microorganisms that can grow only with red light, for example.
  • a frame 80 which is an example of a holding unit, is a frame member that supports the carrier unit 10 and the like, and has a plurality of vertical columns and horizontal beams.
  • the frame 80 in the illustrated example has a mounting portion 80A on the upper side of the frame 80, for example, a beam portion, to which the carrier unit 10 is removably mounted.
  • the carrier unit 10 is supported by the frame 80.
  • the carrier unit 10 is detachably suspended from the frame 80.
  • the control unit 90 is configured by a computer or the like, and controls components of the culture system 1.
  • the control unit 90 controls the operation timing of the roller unit 30 and the culture solution supply unit 50.
  • the microorganism to be cultured is, for example, photosynthetic microalgae.
  • the culture system 1 may not include the irradiation unit 70.
  • the culture solution is not particularly limited as long as it is a diluent of a medium capable of increasing the concentration of microorganisms by culturing microalgae by an ordinary method.
  • a common inorganic medium such as a CHU medium, a JM medium, and an MDM medium can be used.
  • diluents of various kinds of medium such as a Gamborg B5 medium, a BG11 medium, and an HSM medium are preferable.
  • Ca (NO 3 ) 2 .4H 2 O, KNO 3 , and NH 4 Cl are used as a nitrogen source, and KH 3 PO 4 , MgSO 4 .7H 2 O, and FeSO 4 .7H are used as other main nutrients. 2 O and the like.
  • An antibiotic or the like that does not affect the growth of the microalgae may be added to the medium.
  • the pH of the medium is preferably 4 to 10. Wastewater or the like discharged from various industries may be used.
  • FIG. 3 is a partially enlarged view of the carrier unit 10.
  • FIG. 4 is a sectional view taken along line IV-IV in FIG.
  • FIG. 5A is a front view of the carrier 12 and the cover 15, and
  • FIG. 5B is a sectional view taken along line Vb-Vb of FIG. 5A.
  • the detailed configuration of the carrier unit 10 will be described with reference to FIGS. 3 to 5B.
  • the carrier unit 10 includes the carrier 12, the cover 15, and the support 17.
  • the carrier 12 is a plate-like member for culturing microorganisms.
  • the carrier 12 can carry microorganisms and can permeate and flow the supplied culture solution.
  • the illustrated carrier 12 is, for example, a cloth member, and more specifically, is preferably formed of, for example, a non-twisted pile fabric.
  • the pile fabric refers to a woven fabric in which a pile is woven on one side or both sides of a knitted fabric.
  • the material of the carrier 12 is not particularly limited, and a pile of twisted yarn, cotton, silk, wool, acrylic, polyester, or the like may be used.
  • the carrier 12 has a substantially rectangular shape when viewed from the front in a developed state.
  • the carrier 12 is bent into two substantially at the center between the opposing long sides (see FIG. 5B).
  • the size of the carrier 12 is not particularly limited, but is formed as large as possible so that microorganisms can be efficiently cultured on one carrier 12.
  • the carrier 12 has a length in the longitudinal direction of 2.5 m when unfolded, and a towel cloth having a length in the width direction of 1 m. Folded in the center.
  • the carrier 12 has a bent portion 125 (an example of a bent portion) which is a portion bent and curved into a U-shaped cross section that is convex upward, and a rectangular shape hanging down from both sides of the bent portion 125. It has a first part 121 and a second part 123. The first portion 121 and the second portion 123 each extend in the up-down direction in a state where the bent portion 125 is hung on the support 17, and their lower edges are substantially horizontal and aligned at substantially the same height. ing.
  • the cover 15 which is an example of the container and the cover will be described.
  • the cover 15 is formed by bending the center of a flexible rectangular sheet member in the longitudinal direction.
  • the cover 15 is made of a material that transmits at least a part of light emitted from the irradiation unit 70. Preferably, the transmittance is as high as possible.
  • the cover 15 is made of, for example, a synthetic resin such as vinyl chloride, polyethylene, polyester, polypropylene, and PET.
  • the cover 15 has a bent portion 155 having a U-shaped cross section that is convex upward, and a first portion 151 and a second portion 153 that hang down from both sides of the bent portion 155.
  • the first part 151 and the second part 153 each extend in the vertical direction in a state where the bent part 155 is hooked on the support 17.
  • the cover 15 has a lower end 156 in a state of being hung on the support 17 and two side edges 157 extending substantially parallel downward from both ends of the bent portion 155 extending in the width direction.
  • the lower end portion 156 has a central portion in the width direction protruding downward in a gentle V-shape. In other words, the lower end 156 extends obliquely downward from the lower end of the side edges 157 toward the center between the side edges 157.
  • the illustrated cover 15 is formed in a flat bag shape that can accommodate the carrier 12 therein.
  • the cover 15 is formed so as to be able to seal the inside except for a part.
  • the lower end portion 156 is closed by fusion
  • the side edge portion 157 is closed by a fixing portion 158 formed of, for example, a hook-and-loop fastener so as to be openable and closable.
  • the illustrated cover 15 has a pair of first openings 159 ⁇ / b> A that are open upward and penetrate a mounted member 175 (described later) at both ends in the width direction of the bent portion 155.
  • the cover 15 has a second opening 159B that opens upward and is penetrated by a culture solution supply pipe 171E (described later) on one side in the width direction of the bent portion 155.
  • the cover 15 has a discharge port 159C at a lower end portion 156 at a distal end projecting downward in a V-shape to discharge a culture solution.
  • the outlet 159C has a tubular shape so that the second pipe 53 for leading out the culture solution can be detachably connected.
  • the support 17 includes a cover support 171 having a horizontal tubular shape that supports the cover 15, a carrier support 173 that extends horizontally and hangs and supports the carrier 12, and a cover 15. It has a mounted body 175 that projects upward through the first opening 159A and is mounted on the carrier unit 10, and an extension plate 176 fixed to the mounted body 175 and extending in the width direction.
  • the support 17 is desirably formed of a material such as aluminum, stainless steel, or plastic that does not hinder the culture of microorganisms.
  • the cover support 171 which is an example of the container support, the supply unit, and the culture solution supply unit will be described.
  • the cover support 171 includes a support rod 171A which is a columnar member extending in the width direction, a cylindrical outer body 171B provided coaxially with a gap around the outer periphery of the support rod 171A, and an outer body formed on the outer body 171B.
  • a culture solution supply hole 171C for supplying a culture solution inside the 171B, a plurality of culture solution discharge holes 171D formed in the outer peripheral body 171B and discharging the culture solution, and a culture solution connected to the culture solution supply hole 171C. It has a supply pipe 171E and support rod ends 171F provided at both ends of the support rod 171A.
  • the outer peripheral body 171B is provided coaxially with the support rod 171A, and both ends are sealed. Thereby, the culture solution can pass between the inner peripheral surface of the outer peripheral body 171B and the outer peripheral surface of the support rod 171A.
  • the outer peripheral body 171B has a culture solution supply hole 171C on one side (the right side in FIG. 3) in the width direction and facing upward.
  • a culture solution supply pipe 171E is provided in the culture solution supply hole 171C. The culture solution is guided into the outer peripheral body 171B via the culture solution supply pipe 171E and the culture solution supply hole 171C.
  • the outer peripheral body 171B has a plurality of culture solution discharge holes 171D on a surface facing downward.
  • a plurality of culture solution discharge holes 171D are arranged side by side at predetermined intervals in the width direction, and two rows of the plurality of culture solution discharge holes 171D are provided in parallel. That is, as shown in FIG. 4, two culture solution discharge holes 171D are provided side by side in the depth direction.
  • the culture solution in the outer peripheral body 171B is discharged outside through the culture solution discharge hole 171D.
  • the carrier support 173 which is an example of the carrier support and the gas supply unit will be described.
  • the carrier support 173 includes a support column 173A that is a horizontal prism-shaped member extending in the width direction along the support rod 171A, and a rectangular cylindrical outer body 173B provided on the outer periphery of the support column 173A.
  • a gas pipe 173C extending in parallel with the support pillar 173A and passing carbon dioxide gas therein; a gas supply hole 173D to which carbon dioxide gas is supplied; and a gas discharge hole 173E formed in the gas pipe 173C and discharging carbon dioxide gas.
  • the outer peripheral body 173B has a stitching hole 173F to which upper ends of both sides of the carrier 12 are sewn, and ends 173G of supporting pillars provided at both ends of the supporting pillar 173A and extending upward.
  • the outer peripheral body 173B is a hollow member having a substantially rectangular cross section.
  • a support column 173A and a gas pipe 173C are provided inside the outer peripheral body 173B.
  • the outer peripheral body 171B has a plurality of gas discharge holes 173E along the center line of the surface facing downward. In the illustrated example, a plurality of gas discharge holes 173E are arranged at predetermined intervals in the width direction.
  • the gas pipe 173C is arranged at a position facing the plurality of gas discharge holes 173E inside the outer peripheral body 173B.
  • the gas pipe 173C has a gas pipe supply hole 173H at a position corresponding to each of the gas discharge holes 173E.
  • One end of the gas pipe 173C is sealed, and the other end is open to form a gas supply hole 173D.
  • the gas pipe 63 is connected to the gas supply hole 173D.
  • the carbon dioxide gas supplied from the gas pipe 63 is guided from the gas supply hole 173D to the inside of the gas pipe 173C.
  • the carbon dioxide in the gas pipe 173C is discharged through the gas pipe supply hole 173H and the gas discharge hole 173E.
  • the mounted member 175 which is an example of the held portion will be described.
  • the mounted body 175 is, for example, a plate-shaped member, and is detachably attached to a horizontal beam of the frame 80.
  • the mounted members 175 are provided on both sides of the support rod 171A in the width direction.
  • the support rod end 171F of the cover support 171, the support column end 173G of the carrier support 173, and the extension plate 176 are fixed to the mounted body 175.
  • the respective positions such as the relative positions of the cover support 171 and the carrier support 173 are fixed via the mounted body 175.
  • the carrier 12 is disposed on the carrier support 173, and the cover 15 covering the carrier 12 is disposed on the cover support 171.
  • the entire carrier unit 10 has a structure that can be handled alone.
  • the carrier unit 175 is detachably connected to the mounting portion 80A of the frame 80 so that the carrier unit 10 is detachably mounted to the frame 80.
  • the connection of the mounted body 175 to the mounting portion 80A can be performed by a known connection technique, and is not particularly limited.
  • the connection of the mounted body 175 to the mounting portion 80A may be configured to be hooked on the mounting portion 80A using a hook or the like, or may be connected to the mounting portion 80A using a bolt, a clip, a snap button, or a so-called latch mechanism. It may be fixed detachably.
  • the extension plate 176 is, for example, a plate-like member.
  • the extension plate 176 is provided on the mounted body 175 and is fixed in a direction to protrude to the opposite side to the cover support 171 with the mounted body 175 interposed therebetween.
  • the extension plate 176 supports the end in the width direction of the cover 15. That is, the extension plate 176 supports the cover 15 together with the cover support 171.
  • the carrier unit 10 can be removed from the frame 80 and a new carrier unit 10 can be mounted.
  • the body to be mounted 175 protruding from the cover 15 may be removed from the mounting portion 80A.
  • the cover 15 whose inside is wet with the culture solution is opened and the hands are stained. Or the culture solution can be prevented from dropping out of the cover 15.
  • an opening is formed in the cover 15 by opening the surface fastener constituting the fixing portion 158, so that the carrier 12 can be removed.
  • the first part 121 and the second part 123 of the carrier 12 are arranged substantially parallel to each other.
  • the first part inner surface 121A of the first part 121 of the carrier 12 (an example of a first surface) and the first part inner surface 123A of the second part 123 (an example of a second surface) face each other and are separated by a distance Ga.
  • the first part 151 and the second part 153 of the cover 15 are arranged substantially parallel to each other.
  • An inner surface 151A of the first portion 151 of the cover 15 and an outer surface 121B of the first portion 121 of the carrier 12 face each other and are separated by a distance Gb.
  • the second portion inner surface 153A of the second portion 153 of the cover 15 and the first portion outer surface 123B of the second portion 123 of the carrier 12 face each other and are separated by a distance Gb.
  • the carrier 12 is arranged in the cover 15 such that, for example, the distance Gb is 0 cm or more and 10 cm or less.
  • the culture solution discharge hole 171D of the cover support 171 is provided at a position facing the bent portion 125 of the carrier 12 hung on the carrier support 173.
  • the bent portion 125 of the carrier 12 is located below the culture solution discharge hole 171D of the cover support 171 in the vertical direction.
  • the culture solution discharge hole 171D of the cover support 171 and the bent portion 125 of the carrier 12 are separated by a distance Gc.
  • the culture solution discharge hole 171D of the cover support 171 discharges the culture solution toward the bent portion 125 of the carrier 12 (see arrow D1 in the figure).
  • the culture solution discharge hole 171D of the cover support 171 is separated from the carrier 12, it is possible to prevent the culture solution discharge hole 171D of the cover support 171 from being clogged with a foreign substance such as a microorganism and obstructing the discharge of the culture solution. You.
  • the gas discharge hole 173E of the carrier support 173 is provided at a position facing the space between the first part 121 and the second part 123 of the carrier 12.
  • the gas discharge hole 173E of the carrier support 173 discharges carbon dioxide from the upper side to the lower side in a space sandwiched by the inner surface 121A of the first part 121 and the inner surface 123A of the second part 123 (arrow D2 in the figure). reference).
  • the gas exhaust hole 173E of the carrier support 173 blows a mixed air containing carbon dioxide into the inside of the carrier 12 folded into two from below the carrier support 173.
  • the carbon dioxide gas discharged into the cover 15 may be prevented from flowing out of the cover 15 or may be allowed to flow out.
  • the carbon dioxide gas in the cover 15 may flow to the outside of the cover 15 via the first opening 159A, the outlet 159C, and the like.
  • a discharge hole for discharging carbon dioxide gas in the cover 15 may be provided in the cover 15 and a discharge pipe may be connected to the discharge hole.
  • the inner surface 121A of the first part 121 and the inner surface 123A of the second part 123 of the carrier 12 are separated by a distance Ga, and carbon dioxide is discharged from the gas discharge holes 173E of the support 173 toward the gap.
  • a gas supply path is formed between the first part 121 and the second part 123, in which gas flows mainly from top to bottom.
  • the first inner surface 151A of the cover 15 and the first outer surface 121B of the carrier 12 are separated by a distance Gb, and the carbon dioxide gas flows through this gap, so that the carbon dioxide gas is also easily supplied to the first outer surface 121B of the carrier 12. Become. That is, by disposing the carrier 12 housed inside the cover 15 without contacting the cover 15, it is possible to guide the carbon dioxide gas to the outer periphery of the carrier 12.
  • the culture solution discharge hole 171D of the cover support 171 is provided at a position facing the bent portion 125 of the carrier 12 hung on the carrier support 173, the culture solution supplied from the culture solution discharge hole 171D is Is supplied to the bent portion 125.
  • the culture solution supplied to the bending portion 125 substantially uniformly penetrates both the first part 121 and the second part 123. That is, the culture solution supplied to the bending portion 125 as one region branches and permeates into the two regions, the first portion 121 and the second portion 123.
  • FIG. 6A is an enlarged cross-sectional view of the carrier 12, and FIG. 6B is an explanatory diagram of a protrusion 127 formed on the surface of the carrier 12.
  • FIGS. 6A and 6B the configuration of the carrier 12 will be described with reference to FIGS. 6A and 6B.
  • the carrier 12 shown in FIG. 6A has a base 126 which is a flat plate-shaped part formed of fibers, and a fibrous member projecting from the surface of the base 126, that is, a number of protrusions 127 formed by raised portions.
  • the illustrated protrusion 127 has an annular portion (loop portion).
  • each protrusion 127 has a root 127A fixed to the base 126, and a tip 127B that is the part of the protrusion 127 that is the most distant from the base 126.
  • the protrusion 127 has a fiber diameter of 1 mm, and the amount of protrusion from the base 126 (see the length in the depth direction and the distance L1) is about 20 mm.
  • the water holding capacity per unit area of the carrier 12 is desirably 0.2 g / cm 2 or more.
  • the tip 127B is movable with respect to the root 127A as the protrusion 127 is elastically deformed. That is, the tip 127B can swing about the root 127A (see arrow D3).
  • the tip 127B is relatively movable in a direction along the base 126 (vertical direction in the figure), and is also relatively movable in a direction of moving forward and backward with respect to the base 126 (depth direction in the figure).
  • the tip 127 ⁇ / b> B of the projection 127 is located below the root 127 ⁇ / b> A. Is possible (see distance L2). In other words, the fur of the carrier 12 is directed from the upper side to the lower side.
  • the culture solution is supplied to the carrier 12 with the tip 127B disposed below the root 127A
  • the culture fluid flows from the root 127A toward the tip 127B (see arrow D4).
  • the culture solution and the microorganisms are suppressed from staying at the root 127A.
  • decay of the culture solution and the microorganism which may occur with the retention of the culture solution and the microorganism, is suppressed.
  • the surface area of the carrier 12 is increased by providing the projections 127 on the base 126. That is, the area of the region in contact with the gas phase in the culture solution present inside the carrier 12 or on the surface of the carrier 12 increases, and the light receiving area of the carrier 12 also increases. With these, the growth of microorganisms on the carrier 12 can be promoted.
  • a porous base material having a large number of concave portions such as a porous sponge, may be used.
  • the shape of the projection 127 is not particularly limited as long as the tip 127B can move with respect to the root 127A and the base 126.
  • the projection 127 does not have to be formed in a ring shape unlike the example shown in the figure.
  • it may be a substantially columnar, substantially conical, flat tongue, or substantially prismatic member, one end of which is supported by the base 126 and the other end of which is swingable.
  • the carrier 12 may be formed by a shirring ground (a cloth obtained by cutting a loop-shaped portion of a pile cloth).
  • FIG. 7 is a schematic configuration diagram illustrating the roller unit 30.
  • the detailed configuration of the roller unit 30 will be described with reference to FIGS.
  • the roller unit 30 includes a lifting unit 31 that moves up and down along the carrier unit 10, and a driving unit 32 that drives the lifting unit 31.
  • the lifting unit 31 and the driving unit 32 will be described.
  • the elevating unit 31 includes a horizontal roller pair 33 that sandwiches both surfaces of the carrier unit 10, a support bar 35 that holds the attitude of the roller pair 33, a first drive mechanism 37 that changes the center distance of the roller pair 33, And a pair of base plates 39 to which the above components are attached.
  • the roller pair 33 has a cylindrical first roller 331 and a second roller 333 that sandwich the carrier unit 10 from both sides.
  • the first roller 331 is attached to the shaft portion 331A, the outer peripheral portion 331B rotatably mounted on the shaft portion 331A, and the outer peripheral surface of the outer peripheral portion 331B and pressed against the outer surface 151B of the carrier unit 10 (see FIG. 4).
  • Resin sheet 331C is attached to the shaft portion 331A, the outer peripheral portion 331B rotatably mounted on the shaft portion 331A, and the outer peripheral surface of the outer peripheral portion 331B and pressed against the outer surface 151B of the carrier unit 10 (see FIG. 4).
  • Resin sheet 331C is attached to the shaft portion 331A, the outer peripheral portion 331B rotatably mounted on the shaft portion 331A, and the outer peripheral surface of the outer peripheral portion 331B and pressed against the outer surface 151B of the carrier unit 10 (see FIG. 4).
  • Resin sheet 331C is
  • the illustrated first roller 331 and second roller 333 are so-called driven rolls that are not connected to a driving source for rotating each of them. If necessary, the first roller 331 and the second roller 333 may be driven to rotate in conjunction with the vertical movement, respectively, so as to weaken or increase the frictional force on the carrier unit 10.
  • the support bar 35 is a substantially columnar member, and is provided so as to sandwich the first roller 331 and the second roller 333 in the vertical direction. In the illustrated example, the support bar 35 is provided at four corners of the base plate 39 so as to surround the first roller 331 and the second roller 333. The support bar 35 suppresses the first roller 331 and the second roller 333 from bending.
  • the first drive mechanism 37 is provided at both ends of the shaft 331A of the first roller 331 and the shaft 333A of the second roller 333.
  • the first drive mechanism 37 changes the shaft 331A of the first roller 331 and the shaft 333A of the second roller 333 between a reference position and an expanded position that is wider than the reference position (arrows in the figure). D5).
  • the first drive mechanism 37 can be configured by a known drive device such as a solenoid, an air cylinder, and a gear mechanism using a motor.
  • the state where the first roller 331 and the second roller 333 are at the reference position may be referred to as the closed state of the roller pair 33.
  • the roller pair 33 in the closed state sandwiches and presses the carrier unit 10 in the thickness direction.
  • the state where the first roller 331 and the second roller 333 are in the expanded position may be referred to as the open state of the roller pair 33.
  • the roller pair 33 in the open state is separated from the carrier unit 10.
  • the reference position is an example of the approach position, and the spread position is an example of the separation position.
  • both ends of the shaft portion 331A of the first roller 331 and the shaft portion 333A of the second roller 333 are respectively urged using an elastic member such as a spring so that the roller pair 33 is closed.
  • the first drive mechanism 37 applies a force opposing the urging force to the shaft 331A of the first roller 331 and the shaft 333A of the second roller 333 to open the roller pair 33.
  • the roller pair 33 is closed by the urging force.
  • the biasing direction can be reversed, the roller pair 33 can be closed by the driving force of the first drive mechanism 37, and when stopped, the roller pair 33 can be opened by the biasing force, or the first pair can be opened without using the biasing force. It is also possible to open and close with the driving force of the driving mechanism 37.
  • the base plate 39 is provided at both ends of the shaft 331A of the first roller 331 and the shaft 333A of the second roller 333.
  • the illustrated base plate 39 is a plate-shaped member, and is formed of, for example, stainless steel.
  • the drive unit 32 includes a guide member 371 that guides the elevating unit 31 along the vertical direction, a wire 373 having one end connected to the elevating unit 31, and an elevating unit connected to another end of the wire 373.
  • the second drive mechanism 375 and the first drive mechanism 37 are examples of a moving unit.
  • the guide member 371 is, for example, a prism-shaped member provided along the vertical direction.
  • the shaft 331A of the first roller 331 and the shaft 333A of the second roller 333 are provided at both ends.
  • the guide member 371 is a guide member of the elevating unit 31 and slidably supports the base plate 39 of the elevating unit 31.
  • the wire 373 is a linear member made of metal. In the illustrated example, the wire 373 suspends and supports the elevating unit 31.
  • the second drive mechanism 375 has a winding unit 375A that winds the wire 373 and a motor 375B that drives the winding unit 375A.
  • the winding unit 375A that has received the driving force from the motor 375B winds the wire 373, so that the elevating unit 31 moves vertically (see the arrow D6 in the figure).
  • FIG. 8A is a schematic configuration diagram showing the operation of the roller unit 30, and FIG. 8B is an enlarged view in VIIIb of FIG. 8A.
  • first roller 331 and second roller 333 are arranged in the closed position and clamp carrier unit 10 in the thickness direction.
  • the operation of the roller unit 30 will be described with reference to FIGS. 1, 2, 7, and 8.
  • the roller unit 30 in the present embodiment extracts microorganisms together with the culture solution by squeezing out the culture solution contained in the carrier 12 of the carrier unit 10.
  • a method of squeezing the culture solution contained in the carrier 12 for example, it is possible to remove the carrier unit 10 from the frame 80, take out the carrier 12 from the removed cover 15, and squeeze the carrier 12.
  • the carrier 12 is squeezed together with the cover 15 using the roller unit 30 while the carrier unit 10 is mounted on the frame 80. By mounting the carrier unit 10 on the frame 80, the operation of culturing microorganisms again using the squeezed carrier 12 can be performed quickly.
  • the roller unit 30 is used to compress the carrier unit 10 in the depth direction, that is, in the thickness direction of the carrier unit 10.
  • the operation of the roller unit 30 for compressing the carrier unit 10 will be specifically described.
  • FIG. 1 it is assumed that the first roller 331 and the second roller 333 face each other with the carrier unit 10 mounted on the frame 80 interposed therebetween.
  • the first roller 331 and the second roller 333 are arranged at the open position, and are separated from the carrier unit 10, respectively.
  • the first roller 331 and the second roller 333 are lower regions of the carrier unit 10 and face, for example, a region lower than the carrier 12 (see FIG. 1).
  • the first roller 331 and the second roller 333 are arranged at the closed position. That is, the first roller 331 and the second roller 333 are arranged at the reference position.
  • the gap between the first roller 331 and the second roller 333 arranged at the reference position is smaller than the thickness of the carrier unit 10, and the first roller 331 and the second roller 333 sandwich and press the carrier unit 10.
  • the carrier 12 is made of a pile, and the cover 15 is made of a flexible sheet member. Therefore, the carrier unit 10 is deformed as the first roller 331 and the second roller 333 press.
  • the inner surface 121A of the first portion 121 and a part of the inner surface 123A of the second portion 123 come into contact with each other. Even after the pressing by the first roller 331 and the second roller 333 is released, the state where the first part inner surface 121A and the second part inner surface 123A are partially in contact may be maintained due to the surface tension of the culture solution. .
  • the carbon dioxide gas is discharged from the gas discharge holes 173E of the carrier support 173, the space between the first part 121 and the second part 123 of the carrier 12 is expanded, and the contact state is released. .
  • the second drive mechanism 375 (see FIG. 2) is driven while the first roller 331 and the second roller 333 sandwich the carrier unit 10 to wind up the wire 373.
  • the first roller 331 and the second roller 333 move up (see arrow D11).
  • the outer peripheral portion 331B of the first roller 331 and the outer peripheral portion 333B of the second roller 333 are driven to rotate (see arrow D13).
  • the two rollers 333 smoothly rise along the carrier unit 10.
  • the operation of the first roller 331 and the second roller 333 will be described while focusing on the relationship with the protrusion 127 of the carrier 12.
  • the first roller 331 (see FIG. 8A) and the second roller 333 rise while compressing the carrier unit 10. That is, the first roller 331 and the second roller 333 move against the fur of the carrier 12. Therefore, as shown in FIG. 8B, the protrusion 127 pressed from the first roller 331 and the second roller 333 via the cover 15 is deformed, and the tip 127B of the protrusion 127 swings.
  • the movement of the tip 127B of the protrusion 127 makes it easier for the microorganisms adhering to the protrusion 127 to separate from the protrusion 127 together with the culture solution.
  • the first roller 331 and the second roller 333 move up while compressing the carrier unit 10 (see the arrow D11 in the figure), and the gap between the first portion inner surface 151A and the second portion inner surface 153A and the base 126 of the carrier 12 (see FIG.
  • the culture solution can be stored in a circle R1 in the figure). This makes it possible to separate the microorganisms adhering to the protrusion 127 from the protrusion 127 by using the accumulated culture solution (see the symbol Lq in the figure), so that the protrusion 127 can be rinsed.
  • the upper surfaces of the first roller 331 and the second roller 333 move toward the base 126 of the carrier 12 (arrows in the figure). D13).
  • the upper surfaces of the first roller 331 and the second roller 333 have a function of bringing the culture solution to the base 126 side. Therefore, more culture solution can be collected between the inner surface 151A of the first part and the inner surface 153A of the second part and the base 126 of the carrier 12. If necessary, the first roller 331 and the second roller 333 can be lowered while compressing the carrier unit 10.
  • the first roller 331 and the second roller 333 press together with the cover 15 containing the carrier 12. Therefore, the carrier unit 10 can prevent the microorganisms and the culture solution from adhering to the first roller 331 and the second roller 333 and from scattering around. This can reduce the loss of the culture solution and increase the collection efficiency of the microorganism.
  • the cover 15 When the microorganisms are cultured in the carrier unit 10, water droplets may adhere to the inner surface (the inner surface 151A of the first part and the inner surface 153A of the second part) of the cover 15, and the cover 15 may become cloudy. When the cover 15 becomes cloudy in this manner, light that is supposed to reach the carrier 12 from the irradiation unit 70 may be blocked by water droplets, and the amount of light received by the carrier 12 may decrease.
  • the first roller 331 and the second roller 333 press the carrier 12 together with the cover 15 as described above, so that the first inner surface 151A and the second inner surface 153A of the cover 15 Is pressed against.
  • the carrier 12 By pressing the first part inner surface 151A and the second part inner surface 153A against the carrier 12, droplets on the first part inner surface 151A and the second part inner surface 153A are removed. As a result, the amount of light reaching the carrier 12 increases. In other words, as compared with the configuration in which the droplets are not removed, even if the light amount of the light irradiated by the irradiation unit 70 is reduced, the light amount of the light reaching the carrier 12 can be secured. Therefore, in the illustrated configuration, the power consumed by the irradiation unit 70 can be reduced. The visibility of the carrier 12 through the cover 15 is improved by removing the droplets on the inner surface 151A of the first part and the inner surface 153A of the second part and removing the fogging.
  • FIG. 9A is a flowchart showing the operation of the culture system 1
  • FIG. 9B is a flowchart showing the recovery operation of the culture system 1.
  • the operation of the culture system 1 will be described with reference to FIG. 9A.
  • the carrier unit 10 is formed by disposing the carrier 12 inside the cover 15 and the carrier unit 10 is held by the frame 80.
  • the microorganisms are attached to the carrier 12 with the fixing portion 158 opened (step 901). After closing the fixing portion 158, the microorganism is cultured on the carrier 12 while supplying the culture solution and carbon dioxide (Step 902). While driving the roller unit 30, the culture solution and the microorganisms held on the carrier 12 are squeezed out of the carrier 12 and collected using the tank 55 (Step 903).
  • the roller pair 33 is arranged at a position facing the lower end 156 of the carrier unit 10 in the up-down direction and is in an open state.
  • the first drive mechanism 37 is driven, and the roller pair 33 is closed (step 911).
  • the supply amount of the culture solution supplied from the culture solution supply unit 50 to the carrier unit 10 is reduced (step 912).
  • the second drive mechanism 375 is driven, and the roller pair 33 is raised (step 913). As the roller pair 33 rises, the microorganisms and the culture solution held on the carrier 12 are squeezed out of the carrier 12. When the roller pair 33 rises to a position facing the carrier support 173 in the up-down direction, the second drive mechanism 375 stops, and the roller pair 33 stops (step 914).
  • the control unit 90 determines whether a supply time, which is a time for supplying a predetermined amount of the culture solution to the carrier 12, has elapsed since the roller pair 33 was stopped (step 915).
  • a supply time which is a time for supplying a predetermined amount of the culture solution to the carrier 12
  • the second drive mechanism 375 is driven, and the roller pair 33 is lowered by its own weight (step 916).
  • the roller pair 33 descends, the microorganisms and the culture solution held on the carrier 12 are squeezed out of the carrier 12.
  • the second drive mechanism 375 stops, and the roller pair 33 stops (step 917).
  • control unit 90 determines whether the roller pair 33 has risen twice (step 918). If the roller pair 33 has not risen twice (NO in step 918), the second drive mechanism 375 is driven, and the roller pair 33 rises (step 913). On the other hand, when the roller pair 33 has risen twice (YES in step 918), the roller pair 33 is opened (step 919), and the collection operation of the culture system 1 ends.
  • the amount of microorganisms to be collected can be increased by raising the roller pair 33 a plurality of times. After the roller pair 33 is raised, the supply time is waited (step 915), so that the carrier 12 holds a large amount of the culture solution and adheres to the carrier 12 using the culture solution held by the carrier 12. Microorganisms can be rinsed.
  • the culture target cultured by the culture system 1 as described above is not only non-motile or poorly photosynthetic microorganisms such as Chlorella, Synechocystis, and Spirulina, but also planktonic Euglena, Chlamydomonas, and Pleurochrysis which move in water with flagella. Is also included.
  • the microorganisms to be cultured in the culture system 1 are extremely diverse. Examples of the main group of microorganisms to be cultured in the culture system 1 include the following A, B, and C types.
  • Eubacteria include non-oxygen-generating photosynthetic bacteria, cyanobacteria that produce oxygen-generating photosynthesis, facultative anaerobic fermentative and non-fermentative bacteria that use organic substances, as well as vegetative, actinomycete, and coryneform bacteria. And spore-forming bacteria.
  • Photosynthetic bacteria include Rhodobacter, rhodospirillum, chlorobium, chloroflexus.
  • Cyanobacteria include Synechococcus, Synechocystis, Spirulina, Arthrospira, Nostock, Anavena, Osylatorria, Ringbia, Ischurage, Watermelon.
  • Escherichia coli and lactic acid bacteria are mentioned as facultative anaerobic fermentative bacteria.
  • Pseudomonas is mentioned as a non-fermentable bacterium.
  • Hydrogen bacteria are mentioned as the inorganic nutrient bacteria. Streptomyces is an actinomycete, and Bacillus subtilis is a spore bacterium.
  • Archaea include thermophiles and highly halophilic bacteria.
  • Thermococcus is a thermophilic bacterium
  • Halobacterium is a highly halophilic bacterium.
  • Other examples include glutamic acid-producing bacteria, lysine-producing bacteria, and cellulose-producing bacteria.
  • microalgae which are eukaryotic photosynthetic microorganisms.
  • Microalgae include green algae, treboxia algae, red algae, diatoms, haptoalgae, macroscopic algae, euglena, and zooxanthellae.
  • Green algae include chlorella, scenedesmus, chlamydomonas, botryococcus, haematococcus, nannochloris, and pseudocolicitis
  • treboxia algae include parachlorella and cocomixa.
  • red algae examples include cyanidiosizon, cyanidium, gardieria, and porphyridium, and examples of diatoms include nichea, pheodactylum, keitokeros, taraciosila, skeletonema, and fituriera.
  • Haptoalgae include Pleurochrysis, Gefilocapsa, Emiliania, Isochrysis, Pavlova.
  • Nannochloropsis is an example of a true alga
  • Euglena is an example of an euglena.
  • zooxanthellae which is a coral symbiotic algae, there is cimbiodinium.
  • examples of C include fungi that are non-photosynthetic eukaryotes.
  • Fungi include yeast and Aspergillus.
  • Mycelium culture of basidiomycetes is a culture target.
  • moss which is a green plant
  • Lichens which are symbiotic organisms, can also be cultured.
  • Microalgae can be considered as containing cyanobacteria.
  • FIG. 10A to FIG. 10D are diagrams illustrating a modification of the present embodiment. Modifications of the present embodiment will be described with reference to FIGS. 10A to 10D. In the following description, the same portions as those of the configuration described in the above embodiment are denoted by the same reference numerals, and detailed description thereof will be omitted.
  • the carrier unit 10 is provided with the cover 15, but the present invention is not limited to this.
  • a configuration in which the cover 15 is not used as shown in FIG. 10A may be used.
  • the first roller 331 (see FIG. 7) and the second roller 333 directly press the carrier 12.
  • the first roller 331 and the second roller 333 are configured to directly press the carrier 12, and the carrier 12, the first roller 331, and the second roller 333 are configured to be covered by the cover 15. You may.
  • spacers may be provided on the inner surface 151A of the first part and the inner surface 153A of the second part of the cover 15 so as to form an interval of 10 cm or less between the cover 12 and the carrier 12.
  • the spacer may be a number of protrusions or ribs attached to the first inner surface 151A and the second inner surface 153A of the cover 15, or integrally formed with the cover 15. This suppresses the first part inner surface 151A and the second part inner surface 153A from being in contact with and adhered to the carrier 12 over a large area.
  • the cover 15 is described as having flexibility. However, the cover 15 is not limited to this as long as the pressing force from the first roller 331 and the second roller 333 can be transmitted to the carrier 12.
  • the cover 15 may be formed of a plate member such as a resin plate that covers the carrier 12 and is more difficult to deform than a sheet member.
  • a single carrier 1200 may be suspended from a flat plate.
  • the carrier 12 may be formed in a shape other than a flat plate such as a cylindrical shape or a rectangular tube shape.
  • the number of carriers 12 is not limited to one, and two or more carriers 12 may be provided.
  • a part of the first part 121 and a part of the second part 123 may be sewn together.
  • three sides other than the bent portion 125 of the carrier 12 folded in two are closed by stitching, leaving a part of the support 17 into which the carrier support 173 is inserted, to form a space inside the carrier 12. May be.
  • Good air permeability is provided between the first portion 121 and the second portion 123 of the carrier 12, that is, between the first portion inner surface 121A of the first portion 121 and the second portion inner surface 123A of the second portion 123 of the carrier 12.
  • a core material may be provided. Thereby, contact between the first part inner surface 121A and the second part inner surface 123A is suppressed.
  • the cover support 171 supports the cover 15 and the carrier support 173 supports the carrier 12, but the support 17 supports both the cover 15 and the carrier 12.
  • the cover support 1710 supports the cover 15 and the carrier 1200 may be employed.
  • the cover support 171 supplies the culture solution to the bent portion 125, but the invention is not limited to this as long as the culture solution can be supplied to the carrier 12.
  • a mode in which the cover support 1710 supplies a culture solution to the upper end 1201 of the carrier 1200 may be used.
  • the carrier support 173 supplies the carbon dioxide gas inside the carrier 12, but the invention is not limited to this as long as the carbon dioxide gas is supplied to the carrier 12.
  • the gas supply body 1730 may have a configuration in which carbon dioxide gas D20 is sprayed on both surfaces of the carrier 1200. More specifically, a configuration for holding the carrier 12 and a configuration for supplying carbon dioxide may be provided separately. Although not shown, a configuration for holding the cover 15 and a configuration for supplying the culture solution may be provided separately.
  • any member that can separate the culture solution and the microorganism from the carrier 12 may be used instead of the roller pair 33.
  • a configuration may be employed in which the carrier 12 is slid while being pressed by a pressing member 3311 (squeegee) having a curved portion 3310 protruding toward the carrier 12.
  • the shapes of the first roller 331 and the second roller 333 are not particularly limited.
  • the portion of the roller pair 3320 having the largest outer diameter, that is, the portion that contacts the carrier 12 may be formed discontinuously in the width direction.
  • the direction of the roller pairs 33 is not particularly limited.
  • a configuration in which the roller pairs 33 are arranged in the vertical direction and moved in the width direction may be employed.
  • the present invention it is possible to improve the work efficiency of exchanging the carrier, and to easily maintain the temperature near the carrier at a temperature suitable for culture. Can be. Therefore, industrial use is possible.

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Abstract

La présente invention concerne un dispositif de culture (1) ayant : une unité formant support (10) pour cultiver une cible de culture ; un cadre (80) qui maintient l'unité formant support (10) ; et une unité d'alimentation de liquide de culture (50) pour recueillir la cible de culture cultivée. L'unité formant support (10) est munie : d'un support (12) pour fixer la cible de culture ; d'un couvercle (15) qui reçoit le support (12) à l'intérieur ; d'un support (17) qui soutient le couvercle (15) et le support (12) depuis l'intérieur du couvercle (15) ; et d'un corps (175) à monter qui est fourni au support (17) et maintenu par le cadre (80).
PCT/JP2019/025158 2018-06-27 2019-06-25 Dispositif de culture, unité de culture, et procédé de collecte de cible de culture WO2020004388A1 (fr)

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JP7345194B2 (ja) * 2018-06-27 2023-09-15 株式会社BrainGild 培養装置、培養対象回収方法、および担体押圧ローラ
JP6530112B1 (ja) * 2018-06-27 2019-06-12 日本曹達株式会社 培養装置、培養ユニット、および培養対象回収方法
JP7440910B2 (ja) 2020-10-30 2024-02-29 株式会社BrainGild 培養装置および培養対象の製造方法

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JP2013021976A (ja) * 2011-07-21 2013-02-04 Ihi Corp 藻類培養装置及び方法
CN204265740U (zh) * 2014-11-27 2015-04-15 福州大学 一种基于毛细管仿生结构的微藻固定化培养装置
JP2016005439A (ja) * 2014-06-20 2016-01-14 学校法人東京薬科大学 微細藻類の培養装置、及び培養方法
JP2019010037A (ja) * 2017-06-29 2019-01-24 日本曹達株式会社 微生物の培養及び回収方法
JP6530112B1 (ja) * 2018-06-27 2019-06-12 日本曹達株式会社 培養装置、培養ユニット、および培養対象回収方法

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JP2013021976A (ja) * 2011-07-21 2013-02-04 Ihi Corp 藻類培養装置及び方法
JP2016005439A (ja) * 2014-06-20 2016-01-14 学校法人東京薬科大学 微細藻類の培養装置、及び培養方法
CN204265740U (zh) * 2014-11-27 2015-04-15 福州大学 一种基于毛细管仿生结构的微藻固定化培养装置
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JP6530112B1 (ja) * 2018-06-27 2019-06-12 日本曹達株式会社 培養装置、培養ユニット、および培養対象回収方法

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