WO2019221531A1 - Composition for improving vaginal health of women comprising poly-gamma-glutamic acid or salt thereof - Google Patents

Abstract

The present invention relates to a composition for improving the vaginal health of women comprising poly-gamma-glutamic acid or a salt thereof. More specifically, the present invention provides a composition for improving the vaginal health of women that inhibits the growth of Candida albicans, which is a major pathogen causing diseases related to the female urinary and reproductive systems, such as vaginitis, itching and odors, by using poly-gamma-glutamic acid. Thus, the composition for improving the vaginal health of women causes less adverse side effects compared to existing treatment methods for the female reproductive system, soaps, and body cleansers, and can used continuously due to excellent safety.

Description

Composition for improving female vaginal health containing polygamma glutamic acid or salts thereof

The present invention relates to a composition for improving female vaginal health containing polygamma glutamic acid or salts thereof, and more particularly, to poly uragera, itching, and odor, including polygamma glutamic acid, according to the present invention. By inhibiting the growth of Candida albicans , a major pathogen, it has no side effects compared to traditional methods of treating urinary system of women and soaps and body cleansers, and it can be used continuously while improving skin elasticity, skin moisture loss and skin tone. The present invention relates to a composition for improving female vaginal health while helping to improve vulva itching and odor relief.

The future development of cosmetics is emphasized the importance of functional cosmetics that have enhanced efficacy and effectiveness compared to general cosmetics that emphasize stability, and the expansion of the range of functional cosmetics in the future is becoming an issue for the future development of the cosmetics industry. In other words, the concept of cosmetics is expected to expand from existing concepts such as skin care, anti-aging and cell regeneration, whitening and UV protection to products of current quasi-drugs and medicinal cosmetics such as wound healing, anti-atopy, and body improvement. The enforcement of the Cosmetic Act and the expansion of the range of functional cosmetics are accelerating the development of R & D and manufacturing technology of cosmetics, and the patent application and approval of functional cosmetics is expected to gradually increase as a cornerstone for preoccupation of the era of functional cosmetics. There is a growing tendency to be recognized for technology through domestic and foreign patent acquisition.

Today, almost everyone uses a wide variety of types and forms of cosmetics, and the cosmetics industry is constantly focusing on researching and developing new materials. Cosmetics have a beneficial effect on the skin, such as skin cleansing, moisturizing, sun protection, wrinkle improvement, whitening and soothing, but can be a major cause of contact dermatitis. Contact dermatitis is a dermatitis caused by contact with an external substance and is classified into irritant contact dermatitis caused by an irritant and allergic contact dermatitis which occurs in a person sensitized to a specific antigen. Therefore, it is also important that functional cosmetics have safety besides having a beneficial function on the skin.

Meanwhile, three-quarters of the world's female population suffers from infections of inflammatory female diseases, including bacterial vaginosis, of which 50% are reported to relapse. In addition, more than 60% of women's yeast infections are asymptomatic infections, and antibiotics, stress, hormonal changes after menopause, and sensitivity to vaginitis increases significantly.

The environment inside the vagina is very good for the growth of bacteria because it is humid, warm and unaffected by light. Therefore, in the normal vagina, a microflora is formed in which various microorganisms are in equilibrium. Especially, the lactic acid bacteria make the vagina weakly acidic and inhibit the growth of pathogens and fungi.

However, when the antibiotics or contraceptives are taken, the device such as tampons, loops, excessive vaginal cleansing, irritation of the vaginal mucosa, etc. Proliferation of opportunistic pathogens, which are inhibited by, occurs, resulting in vaginitis.

Vaginitis is divided into candida vaginitis, trichomonas vaginitis and bacterial vaginitis. Candida vaginitis is the most common form of vaginitis caused by the fungus Candida albicans , which causes at least one vaginal and vulvar candida vaginitis more than once during approximately 75% of women's lives. It is common enough to cause a relapse two or more times. The major pathogen of bacterial vaginitis is known as Gardnerella vaginalis , which is caused by the loss of lactic acid bacteria that normally exist in the vagina.

Imidazoles such as polyene-based nyststin, amphotericinB, azole-based ketoconazole, miconazole and Chemosynthetic antibiotics such as triazoles such as itraconazole and fluconazole are commonly used and have a high treatment rate of 70%, but have been found to recur within 30 months (30%). KK, Bacterial vaginosis and women and babies, Int. J. Gynaecol. Obstet., 67 Suppl 1, S9-11 (1999)).

Such chemically synthesized antibiotics may cause more problems in subsequent treatment due to resistance, and may also cause secondary complications because they kill the bacterial flora including lactic acid bacteria in the vagina, which may cause additional infection.

Vaginal cleansing agents also often contain povidone-iodine and detergents for detergency, which are known to be effective in the prevention and treatment of vacandidal vaginitis, trichomonas vaginitis and other vaginal infections. Povidone-iodine is known to cause elevated levels of inorganic iodine or total blood iodine in the vagina, leading to hypothyroidism, and mucosal damage or dermatitis due to iodine hypersensitivity may occur. In addition, it is difficult to expect a fundamental therapeutic effect in the case of simply cleaning the vaginal acidity.

Therefore, there is a need for a composition for preventing or treating vaginitis without the irritation and side effects derived from natural products that can overcome the problems of the synthetic antibiotics and cleaning agents.

On the other hand, polygamma glutamic acid (γ-PGA) is a mucus material produced by microorganisms. Poly-gamma-glutamic acid is produced from Bacillus isolates from Cheonggukjang, a traditional Korean fermented soybean meal using rice straw. Polygamma glutamic acid is an edible, water-soluble, anionic and biodegradable polymer that can be used as a raw material and functional food material for cosmetics such as moisture absorbents and moisturizers.

The present inventors produce polygamma glutamic acid using a high-molecular weight polygamma glutamic acid and a material patent for the method of using the same (Korean Patent No. 399091), a salt-tolerant strain Bacillus subtilis Chungkukjang strain producing high molecular weight poly gamma glutamic acid. Acquired a patent for a method (Korean Patent No. 500796), and a patent for anti-cancer composition containing polygamma glutamic acid, an immunoadjuvant, an immune enhancer, and a virus infection inhibition (Korean Patent No. 496606, Republic of Korea) Patent No. 517114, Republic of Korea Patent No. 475406, Republic of Korea Patent No. 0873179). In addition, by revealing the anti-cancer function through the immuno-enhancing polygamma glutamic acid [Poo, H.R. et al., Journal of Immunology, 178: 775, 2007, Poo, H.R. et al., Cancer Immunol Immunother, 59:11, 2010], and studies on the medical use of polygamma glutamic acid have been carried out to develop various uses for polygamma glutamic acid and to study various effects.

The cosmetic composition containing the conventional polygamma glutamic acid provides an application aspect as a moisturizing (Korean Patent Publication No. 2010-0101328), skin whitening (Korean Patent Publication No. 2010-0086728) or for enhancing the skin absorption of minerals (2009-0132372). However, it has not been applied to improve women's quality health.

Accordingly, the present inventors have made efforts to confirm the effects of polygamma glutamic acid on the improvement of female vaginal health, and thus successfully manufactured a cosmetic composition for improving female vaginal health containing polygamma glutamic acid or its salts, and this material causes the vaginitis. Inhibits the growth of Candida albicans , helps improve skin elasticity, skin moisture loss, skin tone, and improves women's quality health with excellent safety while improving vulva itching and odor relief. The invention was completed.

An object of the present invention is to provide a composition for improving female vaginal health containing polygamma glutamic acid or a salt thereof which is effective for improving female vaginal health.

The present invention relates to an antimicrobial composition having an antimicrobial or antifungal effect composed of polygammaglutamic acid or a salt thereof.

The composition may be an antimicrobial composition having antifungal activity against candida vaginitis. Specifically, the candida vaginitis may be vaginitis caused by Candida albicans.

The present invention relates to a deodorizing composition having a deodorizing effect composed of polygammaglutamic acid or a salt thereof.

The deodorizing effect may be to remove the odor caused by the ammonia-based material.

The salt may be selected from the group consisting of sodium salt, potassium salt, calcium salt, magnesium salt, ammonium salt and zinc salt.

In addition, the present invention relates to a cleaning composition for women comprising the composition as an active ingredient.

The antimicrobial composition may be included in an amount of 1 to 20% by weight based on the total weight of the female detergent composition.

The female detergent composition may further include any one or more additives selected from the group consisting of surfactants, moisturizers, conditioning agents, thickeners, neutralizers, pigments and flavorings.

The female detergent composition may be a detergent, a pharmaceutical, or a cosmetic for preventing or treating candida vaginitis.

For the cleaning agent may be any one selected from the group consisting of a spray, solid, liquid, gel soap or foam type cleaner.

The cosmetics may be formulated into any one selected from the group consisting of lotion, lotion, cream, essence, powder, aerosol, oil or mist.

In the present invention, the antimicrobial composition may mean a composition having excellent antifungal activity against Candida albicans , a fungus that causes vaginitis, and a substance capable of inhibiting or inhibiting the development and life functions of bacteria and fungi. it means.

 The antimicrobial composition according to the present invention is not particularly limited in use, and may be used, for example, in women's cleaning composition for preventing or treating vaginitis. In addition, the cleaning composition for women can be used for cleaning, pharmaceutical, cosmetics, wet wipes and the like.

In addition, the cleaning agent may be formulated in a spray type, liquid type, gel type, powder, solid soap, foam type, and the like, and the cosmetics may be arbitrarily selected in the formulation, but a lotion, lotion, It may be made of cream, essence, aerosol, powder, oil or mist.

At this time, the vaginitis is Candida vaginitis, the causative agent of Candida vaginitis may be Candida albicans .

When the antimicrobial composition according to the present invention is used in a female detergent composition, it is preferable that 1 wt% to 20 wt% of the total weight of the female detergent composition is included. If the content is less than 1% by weight, there is a problem in that it is difficult to expect a substantial antibacterial and antifungal effect, and when the content exceeds 20% by weight, there is a significant value in that it is difficult to manufacture a product suitable for the formulation.

In addition, in the present invention, in addition to the polygamma glutamic acid or salts thereof, the cosmetic female cleanser composition includes various components generally used for external preparations for skin, for example, water-soluble, within the range of not deteriorating the effect of the present invention if necessary. It is possible to mix | blend a component, a powder component, an oil, surfactant, a moisturizer, a viscosity modifier, a preservative, antioxidant, a fragrance | flavor, a pigment, etc. In the present invention, the female cleanser composition for cosmetics may further include at least one additive selected from the group consisting of oil, water, surfactants, humectants, thickeners, chelating agents, pigments, preservatives, and fragrances. have.

On the other hand, the present invention, the antimicrobial composition 0.1 to 20% by weight; 0.1 to 0.2 wt% surfactant; 0.01-0.02% by weight of alkali metal benzoate; And it can provide a spray type cleaning composition for the prevention or treatment of vaginitis including the remaining purified water.

And, the present invention, the antimicrobial composition 0.1 to 20% by weight; 5-15% by weight of dimethicone copolyol; 5-15% cyclomethicone; Urea 10-30 wt%; Glycerin 10-30% by weight; 0.01 to 0.03 weight percent of triethanolamine; 0.01 to 2 wt% preservative; And it can provide a gel detergent composition for the prevention or treatment of vaginitis including the remaining purified water. At this time, the gel may be a detergent such as soap, it may be applied in an integral form of the detergent composition as a gel formulation.

In addition, the present invention, the antimicrobial composition 0.1 to 20% by weight; Sodium lauryl sulfate 1-3 wt%; Urea 0.5-1.5 wt%; L-menthol 0.2-0.7 wt%; 0.6 to 1% by weight of polyoxyethylene nonylphenyl ether; Ethanol 10-20 wt%; EDTA 0.02-0.08 weight percent; 0.01 to 2 wt% preservative; And it can provide a liquid detergent composition for the prevention or treatment of vaginitis comprising the remaining purified water. In this case, the liquid form may be a detergent such as liquid soap, and may be applied in an integral form of the detergent composition as a liquid formulation. In addition, the liquid formulation may be applied to a non-woven fabric or pulp in the form of a wet tissue.

In addition, the present invention, the antimicrobial composition 0.1 to 20% by weight; 10 to 15 weight percent wax; Glycerin, fatty acid ester 5-10% by weight; 0.1 to 1 weight percent aluminum phosphate; 0.1 to 1% by weight of ferric phosphate; 10-30 weight of sorbitol; It may be a detergent such as a soap containing a whole amount of purified water.

In addition, the antimicrobial composition according to the present invention may be used for cosmetic compositions including antibacterial and antifungal functions, skin cleansing agents having antibacterial and antifungal functions, antiseptics having antibacterial and antifungal functions, and pharmaceuticals comprising the natural extractive antimicrobial compositions. Pharmaceutical products, cosmetics, detergents, disinfectants, wet wipes and the like can be provided.

Specifically, the antimicrobial composition according to the present invention can be added to the female vaginal health improvement functional cosmetics. In the composition for improving female vaginal health of the present invention, the content of polygamma glutamic acid is preferably 0.005% to 10%. When the content is 0.005% or less, the female vaginal health improvement effect cannot be expected. Not only can not be expected to improve health, but it is also costly and not economical.

Cosmetics prepared from the antimicrobial composition of the present invention may be prepared in the form of general emulsion formulations and solubilized formulations. Emulsifier-type cosmetics include nutrient cosmetics, creams, essences, etc., and solubilized formulation cosmetics include soft cosmetics.

In addition, the compositions of the present invention may be prepared in adjuvant form for topical or systemic application commonly used in the field of dermatology by containing a dermatologically acceptable medium or base in addition to cosmetics.

Formulations of suitable cosmetics include, for example, emulsions, suspensions, emulsions, pastes, microemulsions, microcapsules, microgranules or ionic (liposomes) obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. , In the form of nonionic vesicle dispersants, creams, skins, lotions, powders, soaps, ointments, sprays, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations or conceal sticks Can be provided. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

“Dermatologically acceptable carriers” that can be used according to the desired formulation include purified water, oils, waxes, fatty acids, fatty alcohols, fatty acid esters, surfactants, humectants, thickening agents, antioxidants, and viscosity stabilizers. Examples include, but are not limited to, topical stabilizers, chelating agents, buffers, preservatives, lower alcohols, and the like, and their types and concentrations are varied and modifications may be made by those skilled in the art without departing from the spirit and scope of the present invention. And parts that can be changed. If necessary, a whitening agent, a moisturizer, an anti-inflammatory agent, an antibacterial agent, an antifungal agent, vitamins, sunscreens, antibiotics, anti-acne agents, perfumes, dyes may be included, and these may be included in the cosmetic composition according to the present invention in an amount commonly used in the cosmetic field. May be included. Suitable from 0.001 to 50% by weight per total weight of the composition.

Specifically, the oil may be hydrogenated vegetable oil, perm oil, cottonseed oil, olive oil, palm oil, jojoba oil, avocado oil, wax, beeswax, carnauba, candelilla, montan, ceresin, liquid paraffin Lanolin may be used. Stearic acid, linoleic acid, linolenic acid, oleic acid may be used as the fatty acid, cetyl alcohol, octyl dodecanol, oleyl alcohol, pantenol, lanolin alcohol, stearyl alcohol, hexadecanol may be used as the fatty acid. As the fatty acid ester, isopropyl myristate, isopropyl palmitate, butyl stearate and the like may be used, but is not limited thereto.

Examples of the surfactant include anionic surfactants such as sodium stearate, sodium cetyl sulfate, polyoxyethylene laurylether phosphate, sodium N-acyl glutamate; Cationic surfactants such as stearyldimethylbenzylammonium chloride and stearyltrimethylammonium chloride; Amphoteric surfactants such as alkylaminoethylglycine hydrochloride and lecithin; Glycerine monostearate, sorbitan monostearate, propylene glycol monostearate, polyoxyethylene oleyl ether, polyethylene glycol monostearate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene coconut fatty acid monoethanol arnide (monoethaolarnide ), Polyoxypropylene glycol, polyoxyethylene castor oil, nonionic surfactants such as polyoxyethylene lanolin and the like.

Glycerin, 1,3-butylene glycol, propylene glycol may be used as the moisture absorbent, and ethanol or isopropanol may be used as the lower alcohol. Examples of thickeners include sodium alginate, sodium caseate, gelatin agar, xanthan gum, starch, cellulose ethers (e.g. hydroxyethyl cellulose, methyl cellulose, carboxymethyl cellulose, hydroxy propylmethyl cellulose), polyvinyl pyrrolidone, Polyvinyl alcohol, polyethylene glycol, sodium carboxymethylcellulose, and the like, but are not limited thereto. As antioxidants, butylated hydroxytoluene, butylated hydroxyanisole, propyl gallate, citric acid, ethoxyquin can be used, and chelating agents include disodium edetate and ethane hydroxy diphosphate. Citric acid, sodium citrate, boric acid, borax, disodium hydrogen phosphate are available as buffers, and methyl parahydroxybenzoate, ethyl parahydroxybenzoate, dihydro as preservatives. Acetic acid, salicylic acid, benzoic acid are available, but are not limited to these.

Moisturizers may also be added to the cosmetic composition. "Moisturizer" refers to a reagent that provides a moisturizing effect to the skin, such as a humectant. Moisturizers are provided in amounts that moisturize. Examples of humectants include glycerin, butylene glycol, propylene glycol, sorbitol, sodium PCA, glucam E-10, glucam E-20 and the like. The moisturizing agent is preferably provided in the range of about 0.1 to about 10% by weight relative to the total weight of the composition, more preferably about 0.1 to about 5% by weight, most preferably about 1 to about 5% by weight. Do.

The composition for improving female vaginal health according to the present invention exhibits excellent antimicrobial activity against Candida albicans , inhibits growth, reduces lesions of candida vaginitis, prevents factors that occur continuously, and skin elasticity. It helps to improve skin moisture loss and skin tone, and it also has the effect of removing vulva itch and odor. In addition, the composition for improving female vaginal health according to the present invention has no skin toxicity and is therefore harmless to the human body and has excellent stability, and thus is useful for improving female vaginal health and can be widely used as a cleaning agent for women.

1 is a graph showing the antifungal activity against Candida albicans of the composition of the present invention according to the colony area change.

Figure 2 shows the antifungal activity against Candida albicans of the composition of the present invention in a graph according to the inhibition rate.

Figure 3 shows the antifungal activity against Candida albicans of the composition of the present invention in a colony area photograph.

Figure 4 graphically shows the CoR value change for the (face) elasticity improvement of the composition of the present invention.

5 is a graph showing the CoR value improvement rate (%) of the (face) elasticity improvement of the composition of the present invention.

6 is a graph showing the change in the transdermal moisture loss amount for improving the skin moisture loss amount of the composition of the present invention.

Figure 7 is a graph showing the percutaneous moisture loss improvement rate (%) for the improvement of the skin moisture loss amount of the composition of the present invention.

8 is a graph showing the change in L * value for improving skin tone of the composition of the present invention.

9 is a graph showing the improvement rate (%) L * value of the skin tone improvement of the composition of the present invention.

Example 1 Preparation of Polygammaglutamic Acid and Its Salts

Basic medium for the production of polygamma glutamic acid (medium with 3% L-glutamic acid; glucose 3%, (NH ₄ ) ₂ SO ₄ 1%, KH ₂ PO ₄ 0.27%, Na ₂ HPO ₄ / 12H ₂ O 0.17% , NaCl 0.1%, sodium citrate 0.5%, Soypeptone 0.02%, MgSO 4 / 7H 2 O 0.7%, vitamin solution (10ml / ℓ, pH 6.8) was prepared by sterilization, 5L fermenter (Jar fermentor, The working volume was fermented by inoculating 4% of the seed culture (LB medium) of Bacillus subtilis var chungkookjang (KCTC 0697BP) in 3 L). The stirring speed was 500rpm, the air injection speed was 1.0vvm, and fermented at 37 ° C. for 48 hours to remove the cells using a filter press (containing diatomaceous earth) to obtain a polygamma glutamic acid-containing sample liquid.

The polygamma glutamic acid-containing sample solution was adjusted to pH 2.0 using 2N sulfuric acid solution and then left at 10 ° C. for 15 hours to obtain a polygamma glutamic acid precipitate. The obtained polygamma glutamic acid precipitate was washed with a sufficient amount of cold distilled water of 10 ° C. or lower (pH 3.5 or more), and then obtained polygamma glutamic acid precipitate by using a Nuche filter, followed by freeze drying to prepare a high molecular weight polygamma glutamic acid.

The polygamma glutamic acid solution was added to distilled water, and sodium hydroxide, potassium hydroxide, calcium hydroxide, and magnesium hydroxide were added to dissolve at a neutral pH to freeze-dry the polygamma glutamic acid salt solution.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

Example 2: Composition Preparation

The polygamma glutamic acid prepared in Example 1 was mixed with the following composition to prepare a composition. This is merely illustrative of the formulations of the present invention, and formulations comprising the compositions of the present invention are not limited in any way.

(1) mist

Rosemary leaf water 30.32 (%), calendula flower 30.32 (%), lemon balm / leaf / stem water 30.32 (%), purified water 3.26 (%), propanediol 2.46 (%), glycerin 1.00 (%), polysorbate 20 0.80 (%), Polyglutamic Acid 0.50 (%), Ersony Extract 0.18 (%), Barberry Fruit Extract 0.18 (%), Pasqueflower Extract 0.18 (%), Capryl Glycol 0.17 (%), Orange Oil 0.06 (%), 1,2-hexanediol 0.06 (%), lactic acid 0.06 (%), lemon oil 0.04 (%), adenosine 0.04 (%), octagonal extract 0.03 (%), lime oil 0.01 (% ), Orange peel oil 0.01 (%)

(2) foam wash

Carbonated water 83.74 (%), glycerin 5.00 (%), coco-betaine 3.00 (%), potassium cocoylglycinate 3.00 (%), sodium cocoylappleamino acid 2.00 (%), sodium lauroyl glutamate 1.00 (% ), Sodium Argan Ampoacetate 1.00 (%), Sodium Benzoate 0.65 (%), Polyglutamic Acid 0.30 (%), Purified Water 0.07 (%), Citric Acid 0.06 (%), Disodium ID 0.05 (%), Tea Tree Leaf Oil 0.04 (%), Potassium OligoylPCA 0.03 (%), Propanediol 0.01 (%), Angelica Extract 0.01 (%), Cheongung Extract 0.01 (%), Green Tea Extract 0.01 (%) , Foliar Extract 0.01 (%), Turmeric Extract 0.01 (%)

Efficacy and effects were evaluated using the composition prepared by the above method.

Test Example 1: Candida albicans ( Candida albicans Antifungal evaluation

Assessment Methods

Candida albicans identification medium (Brilliance TM Candida agar, Oxoid, UK) and an image analysis program (Image J, National Institutes of Health, USA) were applied to evaluate the antimicrobial activity of the composition prepared by the present invention. Candida albicans (ATCC # 10231) was inoculated into sterile medium using sterile test instruments and incubated at 25 ° C. for 24 hours. The test subjects disinfected both palms with 70% ethanol, and the investigators spread 0.2 ml of the culture solution on both palms of the test subjects using a sterile scraper and dried for 1 minute. The right palm (control) was lightly contacted with the hand plate for 3 seconds, the hand was washed with the present composition, and then the left palm (test group) was lightly touched with the hand plate for 3 seconds. The hand plate was incubated for 24 hours at 25 ℃ to analyze the colony area of the formed bacteria to evaluate the inhibition rate against the bacteria compared to the control. Cultured strains were determined to be Candida albicans positive after identification experiment. Using the image analysis program, the colony area (discoloration area) of the bacteria formed on the hand plate of the test group and the control group was quantified, and the antimicrobial activity was evaluated by expressing the inhibition rate of the test substance as a percentage.

result

The results of evaluating the antimicrobial activity against Candida albicans of the test group and the control group are as follows (Tables 1 to 3 and FIGS. 1 to 3). Table 1 shows the Candida albicans colony area will change the (Candida albicans) (N = 20), Table 2 will showing the inhibition rate (%) for Candida albicans (Candida albicans) compared to the control group, compared to the control group Table 3 Statistical analysis of antimicrobial activity against Candida albicans .

As a result of analyzing the colony area of Candida albicans , the inhibition rate against the bacteria in the test group was 94.54%. Was also found to be excellent in antifungal activity to show a statistically significant just after use, the test substance compared to the control (p <.001) the test substance is Candida albicans (Candida albicans)

Test Example 2: (Appearance) Elasticity Improvement Evaluation

Assessment Methods

Ballistometer (Ballistometer BLS780, Dia-Stron Ltd., UK) was applied to evaluate the (surface) elasticity improvement of the composition prepared by the present invention, and the left armpit area of the subject was measured. The ballistometer calculates the skin elasticity by quantifying the vibration energy when the 2 mm diameter arm attached to the probe contacts the skin surface. The analysis was performed using MApp, a ballistometer analysis program. As a measure of skin elasticity, CoR (Coefficient of Restitution), a recovery factor, was used, which represents the ratio of impact velocity to arm rebound velocity. The average value of CoR for the first three bounces was calculated and the maximum was 1.0. As the measured value increased compared to before using the test substance, the (elastic) elasticity was improved. Instrument measurements were taken before the test substance and after 2 weeks and after 4 weeks.

result

The results of evaluating the (elastic) elasticity improvement before and after 2 weeks of use of the test substance using the ballistometer and after 4 weeks of use are as follows (Tables 4 to 6 and FIGS. 4 to 5). Table 4. CoR value change (N = 22) is shown, Table 5. CoR value improvement rate (%) is shown, Table 6. CoR value statistical analysis is shown.

 As a result of analyzing the (elastic) elasticity improvement of the left armpit area by using the ballistometer, the CoR value indicating skin elasticity was increased by 1.62% after 2 weeks and 3.66% after 4 weeks compared with the test substance. Indicated. In addition, it was statistically significant (p <.001) after 2 weeks of use and 4 weeks of use compared to before the use of the test substance.

Test Example 3: Evaluation of Skin Moisture Loss Improvement

Assessment Methods

Vapometer (Delfin Technologies Ltd., Finland) was applied to evaluate the skin moisture loss improvement of the composition prepared by the present invention. Percutaneous moisture loss was measured by contacting the probe to the left armpit area of all subjects at the same pressure. Vapometer measures the evaporation rate by increasing the relative humidity (RH) in the chamber through the humidity sensor in the measuring chamber, and the unit of measurement is g / m2 / h. As the measured value decreased compared to before the test substance was used, the skin moisture loss amount was improved. Instrument measurements were taken before use of the test substance, after 2 weeks, and after 4 weeks of use.

result

The results of evaluating the improvement of the skin moisture loss after using the Vapometer and after 4 weeks of use of the test substance are as follows (Tables 7 to 9 and FIGS. 6 to 7). Table 7 shows the change in transdermal moisture loss (N = 22), Table 8 shows the percentage improvement of transdermal moisture loss, and Table 9 shows the statistical analysis of transdermal moisture loss. As a result of analyzing the improvement of skin moisture loss in left armpit area by using Vapometer, transdermal water loss decreased by 12.49% after 2 weeks and 23.19% after 4 weeks. In addition, it was statistically significant after 2 weeks and 4 weeks after the use of the test substance (p <.05). It was confirmed that the test substance helped to improve skin moisture loss.

Test Example 4: Evaluation of Skin Tone Improvement

Assessment Methods

SkinColorCatch (Delfin Technologies Ltd., Finland) was applied to evaluate skin tone improvement of the composition prepared by the present invention. All probes were measured by contacting the probe vertically to the left armpit area, and white LED light was irradiated at 45 ° to detect the reflected light on the skin with an RGB color sensor. SkinColorCatch measurement consists of 5 factors: L *, a *, b *, melanin index and erythema index, where L * value is brightness, a * value is redness, and b * value is Yellowness, melanin index indicates the amount of melanin present in the skin, and erythema index indicates the amount of skin erythema. As a value for measuring skin tone improvement of the test substance, L * value representing the brightness of the skin was used in the analysis, and it means that the skin tone was improved as the measured value increased compared with before using the test substance. Instrument measurements were taken before use of the test substance, after 2 weeks, and after 4 weeks of use.

result

The results of evaluating skin tone improvement before and after 2 weeks of use of the test substance using SkinColorCatch and after 4 weeks of use are as follows (Tables 10 to 13 and FIGS. 8 to 9). Table 10 shows L * value change (N = 22), Table 11 shows ΔL * value change (N = 22), Table 12.L * value improvement (%), Table 13.L * value statistical analysis It is shown.

As a result of analyzing the skin tone improvement of the left armpit area using SkinColorCatch, the L * value representing the brightness of the skin increased by 1.43% after 2 weeks of use and 2.36% after 4 weeks of use compared to before the test substance. It was. In addition, it was statistically significant (p <.001) after 2 weeks and 4 weeks after the use of the test substance, and it was confirmed that the test substance helped to improve skin tone.

Test Example 5: Evaluation of Deodorizing Effect

Assessment Methods

In order to test the deodorizing effect of the composition prepared by the present invention, the concentration reduction rate of ammonia or trimethylamine was evaluated. 20 ml of the composition sample prepared according to the invention was placed in a 5 L reactor and sealed. The concentration of test gas, ammonia or trimethylamine, was injected at an initial concentration of 50 μmol / mol, and the concentration of the test gas was measured at an initial stage (0 minutes), 30 minutes, 60 minutes, 90 minutes, and 120 minutes (test group). During the test, the temperature was maintained at (23.0 ± 5.0) 'C and the humidity at (50 ± 10)% R.H. Separately, the above experiment was repeated in the absence of the composition prepared by the present invention (control).

result

The concentration reduction rate of the test gas at each time zone was calculated by the following Equation 1.

Table 14 shows the deodorant test evaluation for ammonia, and Table 15 shows the deodorant test evaluation for trimethylamine. As described above, the composition prepared according to the present invention was found to reduce the concentration of ammonia and trimethylamine, and was effective in deodorization.

Test Example 6: Evaluation of skin primary irritation by skin patch test

Assessment Methods

In order to evaluate the skin primary irritation by the skin patch test of the composition prepared according to the present invention, a skin patch test was conducted on 31 subjects using the Finn Chamber. After wiping the back part of the subject with 70% ethanol and drying, the filter paper disc was placed in a Finn Chamber having a diameter of 8 mm, and 20 μL of the test substance was dropped and fixed by attaching to the test part. The patch was attached for 24 hours, and after removal, the test site was marked with a skin marker pen (DeRoyal, Inc., USA).

Determination was performed 30 minutes, 24 hours, 48 hours after removal of the patch by a dermatologist to observe the degree of skin irritation according to the criteria of the International Contact Dermatitis Research Group (ICDRG) of Table 1. The degree of stimulation of the test substance was finally determined.

result

Table 16 shows the skin patch test results. Test substance was not observed irritation 30 minutes, 24 hours, 48 hours after patch removal. The average skin reactivity was 0.00, which was confirmed to be irritant according to the criterion.

According to the present invention, polygamma glutamic acid or salts thereof can be used to prevent and improve / relieve the effect of female genitourinary tract diseases, and the female cleaning composition comprising polygamma glutamic acid or salts thereof according to the present invention has vaginitis, itching By effectively inhibiting the growth and proliferation of Candida albicans , a female urinary tract disease-causing bacterium causing female urogenital system diseases such as odor, it is possible to prevent and improve / relieve female genitourinary diseases. In addition, polygamma glutamic acid is a mucus material produced by microorganisms, and according to the present invention, there is no side effect compared to the existing method of treating the female genitourinary system, and it is possible to obtain a composition for female cleanliness with excellent safety and continuous use.

As described above in detail specific parts of the present invention, it will be apparent to those skilled in the art that these specific descriptions are merely preferred embodiments, and thus the scope of the present invention is not limited thereto. will be. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

According to the present invention, by suppressing the growth of Candida albicans , a major pathogen causing diseases of the female genitourinary system such as vaginitis, itching and odor using polygamma glutamic acid, conventional methods for treating female genitourinary system and soaps It has no side effects compared to body cleansers, and it can be used continuously, but it helps to improve skin elasticity, skin moisture loss, skin tone, and improves female vaginal health improvement while improving vulva itch and odor. Availability will be appreciated.

Claims (12)

1. An antimicrobial composition having an antimicrobial or antifungal effect comprising polygamma glutamic acid or a salt thereof.
2. The antimicrobial composition according to claim 1, wherein the composition has antifungal activity against candida vaginitis.
3. According to claim 1, wherein the candida vaginitis is an antimicrobial composition, characterized in that vaginitis caused by Candida albicans (Candida albicans).
4. Deodorizing composition which has a deodorizing effect containing polygamma glutamic acid or its salt.
5. 5. The deodorizing composition according to claim 4, wherein the deodorizing effect removes odor caused by ammonia-based material.
6. The composition of claim 1 or 4, wherein the salt is selected from the group consisting of sodium salts, potassium salts, calcium salts, magnesium salts, ammonium salts and zinc salts.
7. A female detergent composition comprising the composition of claim 1 as an active ingredient.
8. The method of claim 7, wherein the composition is a female detergent composition, characterized in that contained in 1 to 20% by weight based on the total weight of the female detergent composition.
9. According to claim 7, wherein the female detergent composition further comprises at least one additive selected from the group consisting of surfactants, moisturizers, conditioning agents, thickeners, neutralizers, pigments and flavorings.
10. The method of claim 7, wherein the female detergent composition for the prevention or treatment of candida vaginitis for cleaning, pharmaceutical or cosmetic composition for women, characterized in that for women.
11. The method of claim 10, wherein the cleaning agent is a female detergent composition, characterized in that any one selected from the group consisting of a spray, solid, liquid, gel soap or foam type cleaner.
12. The method of claim 10, wherein the cosmetic composition is a female detergent composition, characterized in that formulated in any one selected from the group consisting of lotion, lotion, cream, essence, powder, aerosol, oil, foam wash or mist.

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