WO2019209105A3 - 7β-HYDROXYSTÉROÏDE DÉSHYDROGÉNASE DÉPENDANTE DE NAD+ - Google Patents

7β-HYDROXYSTÉROÏDE DÉSHYDROGÉNASE DÉPENDANTE DE NAD+ Download PDF

Info

Publication number
WO2019209105A3
WO2019209105A3 PCT/NL2019/050228 NL2019050228W WO2019209105A3 WO 2019209105 A3 WO2019209105 A3 WO 2019209105A3 NL 2019050228 W NL2019050228 W NL 2019050228W WO 2019209105 A3 WO2019209105 A3 WO 2019209105A3
Authority
WO
WIPO (PCT)
Prior art keywords
acid
nad
dependent
oxo
hydroxysteroid dehydrogenase
Prior art date
Application number
PCT/NL2019/050228
Other languages
English (en)
Other versions
WO2019209105A2 (fr
Inventor
Fabio TONIN
Isabella Wilhelmina Christina Everdina Arends
Original Assignee
Technische Universiteit Delft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technische Universiteit Delft filed Critical Technische Universiteit Delft
Publication of WO2019209105A2 publication Critical patent/WO2019209105A2/fr
Publication of WO2019209105A3 publication Critical patent/WO2019209105A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01201—7-Beta-hydroxysteroid dehydrogenase (NADP+) (1.1.1.201)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne des 7β-hydroxystéroïde déshydrogénases dépendantes de NAD+ et des procédés de fourniture d'une 7β-hydroxystéroïde déshydrogénase ayant une spécificité de co-substrat de NAD+ au lieu de NADP+. L'invention concerne en outre des procédés de conversion de l'acide cholique (CA) et/ou de l'acide chénodésoxycholique (CDCA) en acide ursocholique (UCA) et/ou de l'acide ursodésoxycholique (UDCA) respectivement, et plus spécifiquement, des procédés de conversion de l'acide 7-oxo-désoxycholique (7-oxo-DCA) et/ou de l'acide 7-oxo-lithocholique (7-oxo LCA) en acide ursocholique (UCA) et/ou en acide ursodésoxycholique (UDCA) respectivement, en utilisant une 7β-hydroxystéroïde déshydrogénase dépendante de NAD+.
PCT/NL2019/050228 2018-04-25 2019-04-18 7β-HYDROXYSTÉROÏDE DÉSHYDROGÉNASE DÉPENDANTE DE NAD+ WO2019209105A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL2020823 2018-04-25
NL2020823A NL2020823B1 (en) 2018-04-25 2018-04-25 NAD+ dependent 7ß-hydroxysteroid dehydrogenase

Publications (2)

Publication Number Publication Date
WO2019209105A2 WO2019209105A2 (fr) 2019-10-31
WO2019209105A3 true WO2019209105A3 (fr) 2019-12-05

Family

ID=62167877

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2019/050228 WO2019209105A2 (fr) 2018-04-25 2019-04-18 7β-HYDROXYSTÉROÏDE DÉSHYDROGÉNASE DÉPENDANTE DE NAD+

Country Status (2)

Country Link
NL (1) NL2020823B1 (fr)
WO (1) WO2019209105A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113025589B (zh) * 2021-04-21 2023-04-07 重庆第二师范学院 3α-羟基类固醇脱氢酶、编码基因及其在催化剂中的应用
CN113462665B (zh) * 2021-06-30 2023-03-10 中山百灵生物技术股份有限公司 一种7α-HSDH酶突变体及其编码基因和应用
CN113604446B (zh) * 2021-08-16 2023-04-07 重庆大学 7α-羟基类固醇脱氢酶St-2-2的突变体R16Q
CN114250205B (zh) * 2021-12-28 2022-11-11 宋建芳 一种具有高热稳定性的7α-羟基类固醇脱氢酶突变体及其应用
CN115521964B (zh) * 2022-09-19 2024-04-12 湖北共同生物科技有限公司 一种甾体激素药物中间体的制备方法
CN115806951B (zh) * 2022-12-05 2023-06-30 中南大学 NADH依赖型7β-羟基类固醇脱氢酶突变体、编码序列、基因工程菌及应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012080504A1 (fr) * 2010-12-16 2012-06-21 Pharmazell Gmbh NOUVEAUX MUTANTS DE 7ß-HYDROXYSTÉROÏDE DÉSHYDROGÉNASE ET PROCÉDÉ DE PRODUCTION D'ACIDE URSODÉSOXYCHOLIQUE
WO2015197698A2 (fr) * 2014-06-24 2015-12-30 Pharmazell Gmbh Nouveaux procédés pour la réduction biocatalytique sur cellule entière, de composés d'acide déhydrocholique, nouvelles formes mutantes de déshydrogénase 7ss-hydroxystéroïde et procédés biocatalytiques améliorés pour préparer de l'acide ursodésoxycholique
WO2016016213A1 (fr) * 2014-07-29 2016-02-04 Pharmazell Gmbh Mutants de la 7-bêta-hydroxystéroïde déshydrogénase et procédés de production d'acide ursodésoxycholique
CN108546691A (zh) * 2018-05-09 2018-09-18 华东理工大学 7β-羟基甾醇脱氢酶突变体及其在制备熊脱氧胆酸中的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
WO1995006722A1 (fr) 1993-09-03 1995-03-09 Japan Tobacco Inc. Procede permettant de transformer une monocotyledone avec un scutellum d'embryon immature
US6369298B1 (en) 1997-04-30 2002-04-09 Pioneer Hi-Bred International, Inc. Agrobacterium mediated transformation of sorghum
WO2007073149A1 (fr) 2005-12-22 2007-06-28 Keygene N.V. Variantes de nucleotides ameliorant les echanges de nucleotides cibles
RU2010101882A (ru) 2007-06-22 2011-07-27 Киджин Н.В. (Nl) Направленная нуклеотидная замена при применении улучшенных модифицированных олигонуклеотидов

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012080504A1 (fr) * 2010-12-16 2012-06-21 Pharmazell Gmbh NOUVEAUX MUTANTS DE 7ß-HYDROXYSTÉROÏDE DÉSHYDROGÉNASE ET PROCÉDÉ DE PRODUCTION D'ACIDE URSODÉSOXYCHOLIQUE
WO2015197698A2 (fr) * 2014-06-24 2015-12-30 Pharmazell Gmbh Nouveaux procédés pour la réduction biocatalytique sur cellule entière, de composés d'acide déhydrocholique, nouvelles formes mutantes de déshydrogénase 7ss-hydroxystéroïde et procédés biocatalytiques améliorés pour préparer de l'acide ursodésoxycholique
WO2016016213A1 (fr) * 2014-07-29 2016-02-04 Pharmazell Gmbh Mutants de la 7-bêta-hydroxystéroïde déshydrogénase et procédés de production d'acide ursodésoxycholique
CN108546691A (zh) * 2018-05-09 2018-09-18 华东理工大学 7β-羟基甾醇脱氢酶突变体及其在制备熊脱氧胆酸中的应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 24 April 2015 (2015-04-24), ZHENG J., GANEZLE M.: "Lactobacillus spicheri short chain dehydrogenase", XP002782871, Database accession no. KJW13206 *
DATABASE UniProt [online] 24 June 2015 (2015-06-24), ZHENG J., GANEZLE M.: "Short-chain dehydrogenase", XP002782870, Database accession no. A0A0F3RT87 *
SIMONE SAVINO ET AL: "Structural and biochemical insights into 7[beta]-hydroxysteroid dehydrogenase stereoselectivity : Stereoselective Steroid Dehydrogenation", PROTEINS: STRUCTURE, FUNCTION, AND BIOINFORMATICS, vol. 84, no. 6, 6 April 2016 (2016-04-06), US, pages 859 - 865, XP055491010, ISSN: 0887-3585, DOI: 10.1002/prot.25036 *

Also Published As

Publication number Publication date
WO2019209105A2 (fr) 2019-10-31
NL2020823B1 (en) 2019-11-05

Similar Documents

Publication Publication Date Title
WO2019209105A3 (fr) 7β-HYDROXYSTÉROÏDE DÉSHYDROGÉNASE DÉPENDANTE DE NAD+
WO2017220486A3 (fr) Biotransformation autonome couplée de l'acide chénodésoxycholique en acide ursodésoxycholique, et nouvelles enzymes mutées applicables dans ledit procédé
Donova et al. Microbial steroid transformations: current state and prospects
NZ603816A (en) Novel 7alpha-hydroxysteroid dehydrogenase knockout mutants and use thereof
RU2014136162A (ru) Способ ферментной регенерации окислительно-восстановительных кофакторов
Martin Microbial cleavage of sterol side chains
Doden et al. Metabolism of oxo-bile acids and characterization of recombinant 12α-hydroxysteroid dehydrogenases from bile acid 7α-dehydroxylating human gut bacteria
AU2011343222B2 (en) Novel 7B-hydroxysteroid dehydrogenase mutants and process for the preparation of ursodeoxycholic acid
Zhao et al. Mycolicibacterium cell factory for the production of steroid-based drug intermediates
ES2389383T3 (es) Procedimiento para la preparación de derivados de esteroides mediante la reducción de compuestos oxoesteroides o mediante la oxidación de compuestos hidroxiesteroides usando una hidroxiesteroide deshidrogenasa
Kollerov et al. Hydroxylation of lithocholic acid by selected actinobacteria and filamentous fungi
RS52444B (en) Yeast strains that produce cholestrol and their uses
Tawthep et al. Isolation of six novel 7-oxo-or urso-type secondary bile acid-producing bacteria from rat cecal contents
Zhang et al. Effective multi-step functional biotransformations of steroids by a newly isolated Fusarium oxysporum SC1301
WO2015197698A3 (fr) Nouveaux procédés pour la réduction biocatalytique sur cellule entière, de composés d'acide déhydrocholique, nouvelles formes mutantes de déshydrogénase 7ss-hydroxystéroïde et procédés biocatalytiques améliorés pour préparer de l'acide ursodésoxycholique
Wang et al. An oxygenase-independent cholesterol catabolic pathway operates under oxic conditions
MY187073A (en) Use of a trichothecene-transforming alcohol dehydrogenase, method for transforming trichothecenes and trichotecene-transforming additive
US20210317502A1 (en) Genetically-Modified Bacteria And Uses Thereof
WO2022039983A3 (fr) Procédés de fabrication de dérivés d'acide cholique et leurs matières premières
Guevara et al. Metabolic engineering of Rhodococcus ruber Chol-4: a cell factory for testosterone production
Tekucheva et al. Cascade biotransformation of phytosterol to testosterone by Mycolicibacterium neoaurum VKM Ас-1815D and Nocardioides simplex VKM Ас-2033D Strains
Sukhodolskaya et al. Bioconversion of 6-(N-methyl-N-phenyl) aminomethyl androstane steroids by Nocardioides simplex
Zhang et al. Progress of 3-ketosteroid Δ1-dehydrogenases for steroid production
Scully et al. Branched-chain alcohol formation from branched-chain amino acids by Thermoanaerobacter brockii and Thermoanaerobacter yonseiensis
JP2023530667A (ja) ステロイド誘導体生産用遺伝子組換え生物

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 19744884

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 19744884

Country of ref document: EP

Kind code of ref document: A2